Next Article in Journal / Special Issue
The ADP-Ribosyl-Transferases Diphtheria Toxin-Like (ARTDs) Family: An Overview
Previous Article in Journal / Special Issue
ARTD10/PARP10 Induces ADP-Ribosylation of GAPDH and Recruits GAPDH into Cytosolic Membrane-Free Cell Bodies When Overexpressed in Mammalian Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Challenges
Volume 9
Issue 1
10.3390/challe9010023
challenges-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

In Vivo Level of Poly(ADP-ribose)

by
Masanao Miwa
1,*,
Chieri Ida
2,
Sachiko Yamashita
1,
Kenichi Kouyama
1,
Yasuhito Kuroda
1,
Takayuki Eguchi
1,
Narumi Ohta
1,
Teruaki Sato
1,
Masataka Tsuda
3 and
Masakazu Tanaka
4
1
Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga 526-0829, Japan
2
Department of Applied Life Sciences, College of Nagoya Women’s University, Nagoya-shi, Aichi 467-8610, Japan
3
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-0046, Japan
4
Center for Chronic Viral Diseases, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima 890-8544, Japan
*
Author to whom correspondence should be addressed.
Challenges 2018, 9(1), 23; https://doi.org/10.3390/challe9010023
Submission received: 10 April 2018 / Revised: 18 May 2018 / Accepted: 18 May 2018 / Published: 22 May 2018
(This article belongs to the Special Issue Poly ADP Ribose Polymerases (PARP) and Post-Translational Modifications)
Browse Figures
Versions Notes

Abstract

:
PolyADP-ribosylation is a post-translational modification that plays key roles in cellular physiological functions and DNA damage responses. PolyADP-ribosylation is finely and dynamically regulated by various enzymes and factors involved in the synthesis and degradation of poly(ADP-ribose) (PAR). To better understand the function of polyADP-ribosylation, it is necessary to quantify and monitor the change of the in vivo level of PAR, the product of polyADP-ribosylation, which is rapidly turning over and kept in quite low level in cells or in organs. Recent developments of potent inhibitors of polyADP-ribosylation is expected to kill BRCA1/2-mutated breast cancer cells and ovarian cancer cells (synthetic lethality). To know the efficacy of these inhibitors in vivo, it is necessary to develop highly sensitive and reproducible methods to know PAR levels within cells or organs. However there have been several difficulties in measuring the physiologically low level of PAR without artefacts. Our experiments recently clarified that the method of sample preparation is very important in addition to the sensitivity and specificity. From reviewing the literature, including ours, we would like to emphasize the importance of the procedures of sample preparation for the assay, in addition to the sensitivity by comparing the reported PAR levels in vivo.

1. Introduction

1.1. What Is PolyADP-Ribosylation?

PolyADP-ribosylation is a post-translational modification that adds a long polymer of poly(ADP-ribose) (PAR) chain to the acceptor proteins [1,2]. The enzymes, PAR polymerases (PARPs, also named ADP-ribosyltransferases-diphtheria toxin-like (ARTDs) [3]) and ADP-ribosyl transferase 2 (ART2), are responsible for synthesis of PAR attached to acceptor proteins using βNAD+ as the substrate [4,5,6,7]. On the other hand, PAR glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) can degrade the PAR polymer from acceptor proteins [8,9,10]. Finally, ADP-ribosyl protein lyase, macrodomain-containing proteins, terminal ADP-ribose protein glycohydrolase 1 (TARG 1) and ARH3 can remove the proximal ADP-ribose residue from the acceptor protein [11,12,13,14] (Figure 1). There are monoADP-ribosylation reaction and polyADP-ribosylation reaction, and they are collectively termed “ADP-ribosylation reaction”, catalyzed by enzymes widely found in animals, plants and bacteria using NAD+ [15]. However, this article is intended to emphasize the importance of measuring the actual amount of PAR found in vivo and to discuss the methods and the resulting PAR levels in vivo.

1.2. Proposed Functions of PolyADP-Ribosylation

There have been many excellent reviews, including historical ones, on polyADP-ribosylation [1,2,16,17,18,19,20,21,22]. On the biological functions of polyADP-ribosylation, various works in vitro and in vivo have been reported using inhibitors of polyADP-ribosylation, gene-knockout animals of responsible enzymes or in vitro experiments with purified PARPs or PARG [23,24,25,26,27]. They include base-excision DNA repair [28], DNA replication [29,30], transcription [20,31,32], chromatin remodeling [33,34], sister chromatid exchanges [35,36], centrosome number regulation [37], telomere regulation [38], cell signaling [39,40,41,42], cell proliferation [23], carcinogenesis [43], neurodegeneration [12,26], immunological defense [23,44], hypoxia and reperfusion damage [45], and cell death [44,46].
There are two types of functions played by polyADP-ribosylation. One is that since polyADP-ribosylation is a modification of the acceptor proteins, the function of the acceptor proteins could be changed significantly in their enzyme activities and the interaction with other proteins. The acceptor proteins in vitro reaction include PARP1, histone H1, histone H2b, RNase and TP53 [40,47,48,49,50,51] and the number of acceptor molecules is still increasing.
The other is that the attached PAR polymer has a big size with large negative charges. Chemical analysis showed that PAR has branched structure [52]. Consistent with this finding, electron micrography showed that the synthesized PAR had a branched shape [53,54]. The diameter of some of the PAR reached 200–300 nm in length. Althaus and his colleagues noticed and proposed that a free PAR chain is bound non-covalently by specified proteins including histones, and involved in the regulation of biological functions [55,56]. In fact, following modules or motifs that recognize specific structures of PAR have been found including PAR binding motif (PBM), macrodomain motif, PAR binding zinc finger (PBZ), WWE domain and OB fold domain [21,57,58,59,60,61,62]. It is likely that PAR functions as a scaffold for macromolecules to support various biological activities.

1.3. Importance to Understand the In Vivo Level of PAR that is Rapidly Turning-Over under Various Biological Conditions

As shown in Figure 1, polyADP-ribosylation is regulated by enzymes involved in the synthesis and degradation of PAR. In addition, as shown by many investigations, the activities of PARP1 or PARP2 is greatly enhanced by the presence of DNA strand breaks [19]. The interaction of PARP1 or PARP2 with other proteins could also regulate the activities of the proteins. For example, the phosphorylated ERK1/2 protein, one of the important cellular signal transduction factors, could stimulate PARP1 enzyme activity without any involvement of DNA [39]. It is also noticed that PAR is rapidly degraded by PARG. Thus, measuring the amount of the specified enzymes or their activities are not enough to understand the polyADP-ribosylation status, and the measurement of the level of PAR, rapidly turning over in physiological conditions, should be measured as a more accurate marker. As are the characteristics of most of the post translational modifications, the speed of changes of the PAR level is quite rapid, being less than 1 min (unpublished). There are about 106 molecules of PARP1, the most abundant and well-studied member of PARP family present in a cell [32]. However, the level of PAR, the reaction product of PARP1, is considerably low, suggesting the necessity of tight and efficient method of regulation of PAR in vivo. For determining the PAR levels in vivo, under unstressed conditions (i.e., without exogenous DNA damaging agents), western blot analysis is not sensitive enough. Many researchers have frequently experienced the situation that the physiological level of PAR is difficult to show by western blot. Artificial synthesis of PAR could occur presumably due to uncontrollable DNA damages occurring during the procedures of cell lysis. Therefore, in addition to developing highly sensitive and specific assay methods, it is necessary to control the lysis conditions keeping the level of DNA damages that might occur during cell lysis and fixation processes before extraction of PAR minimal.
We will review efforts by various researchers including us to quantitate physiological levels of PAR without apparent exogenous DNA damaging agents to better understand the nature of polyADP-ribosylation and for clarification of its possible biological functions thus far unknown.

1.4. Measurement of the Amount of PAR In Vivo (i.e., in Cells or Tissues)

Although PAR is clearly observable as a smear by western blot analysis after treatment of exogenous DNA damaging agents, the level of PAR in unstressed conditions is quite low and difficult to show by western blot as stated in the previous section. Considering that PARP1 could be activated in the absence of DNA [39], it seems quite interesting to know other physiological functions of polyADP-ribosylation that are unrelated to the exogenous DNA damage and repair. There have been many works suggesting the physiological functions of polyADP-ribosylation using various kinds of PARP inhibitors. However, we do not know exactly how efficient these inhibitors worked in the cell. If the inhibition was not complete, it will cause confusions on the function of PARP1.
One of the difficulties of measuring the physiologically low level of PAR is due to the fact that the substrate of PARPs, NAD+, is impermeable through cell membranes and radioactive NAD+ could not be used for efficient and sensitive labeling of the intracellular PAR. Therefor the precursors of NAD+, e.g., phosphate or adenine, have been used to prove the natural occurrence of PAR. Doly et al. injected [32P]H3PO4 into chicken and isolated liver nuclei. Then, according to the procedure of purifying PAR, already known in vitro, they isolated and purified the PAR fraction and hydrolyzed it with snake venom phosphodiesterase (pyrophosphatase) and found Ado(P)-Rib-P, which is the specific hydrolysis product of PAR (Figure 2). This is the first indication that PAR is synthesized in vivo [63]. The other researchers used radiolabeled adenine to show the natural occurrence of PAR [64]. Subsequently, there have been several reports on the natural occurrence of PAR. Interestingly, naturally occurring antibody against PAR was found in the sera of patients with systemic lupus erythematosus, an autoimmune disease [65]. However, accurate quantitation of PAR has been facing various problems.
As shown in Table 1, Smith and Stocken reported that histone F1 purified from rat liver contained adenine, ribose, phosphate, and serine as 0.89, 1.73, 1.83 and 0.31 nmol per mg protein, respectively. They suggested that ADP-ribose was bound to histone F1 via serine or serine phosphate [66].
Then Stone et al. initially used orthodox isotope dilution methods to quantitate endogenous PAR as 5.59 and 6.32 nmol/mg DNA, for adult and neonatal rat liver, respectively [67] (Table 1). However, the amount of endogenous PAR seems significantly higher than that was reported in the later publication by the same research group [68,69] (Table 1). This might be partly due to the procedure of calculating the specific activity of standard [3H]PAR. [3H]PAR was synthesized from [3H]NAD+ using the sonicated whole cell lysate of Ehrlich ascites carcinoma cells as the enzyme source that might have had already contained significant amount of unlabeled endogenous NAD+. These endogenous NAD+ in the cell lysate might dilute the specific activity of the originally used [3H]NAD+, making the specific activity of the standard [3H]PAR significantly lower than that of the originally used [3H]NAD+. This should cause overestimation of endogenous PAR level.
Kidwell and Mage made rabbit polyclonal antibody against PAR with a mean chain length of 24 after the method by Kanai et al. [70] and developed a radioimmunoassay system to determine PAR levels in HeLa cells [71] (Table 1). They lysed cultured HeLa cells in 0.1 N NaOH for 2 h at 37 °C, stored overnight at 4 °C, neutralized, treated with DNase I and micrococcal nuclease, digested with Pronase and extracted PAR with phenol to be used for the assay. To confirm the specificity of their radioimmunoassay, they showed that the binding of [14C]PAR to the antibody was inhibited with equal amount of unlabeled PAR by 50%, but 25,000-fold excess (w/w) amount of poly(A), native DNA or denatured DNA did not show any appreciable inhibition. Neither NAD+, NADP+, NADH nor NADPH appreciably inhibited the binding when present in the binding assay with a 100,000-fold excess amount of radiolabeled PAR. With ADP-ribose present at a 10,000-fold excess, the binding of labeled PAR was inhibited by 30% and, at a 100,000-fold excess, the inhibition was nearly total. They also labeled cells with [14C] ribose and the materials which bound to the antibody was digested with snake venom phosphodiesterase and separated by Dowex-formate column chromatography. Then they isolated the radioactive compound, which was identified as Ado(P)-Rib-P by Dowex-formate column chromatography as well as PEI-cellulose thin layer chromatography. Using the above specificity of the antibody, they reported the endogenous level of PAR as 3.7 pmol/106 HeLa cells (200 ng/108 cells) (Table 1). Interestingly, there was a small peak of PAR at S phase and a large peak at G2 phase. However, the amount of endogenous PAR was about 100 times higher as compared to the report on HeLa cells by Martello et al. [72] or Ida et al. [73]. The reason for this discrepancy is not clear but might partly be due to the experimental condition to prepare the samples before the assay (discussed later).
Sakura et al. measured PAR with radioimmunoassay using calf thymus nuclei [74] (Table 1). They made rabbit polyclonal antibody against PAR with a mean chain length of 28 (it was later found that this PAR contained much higher molecular weight PAR with branches). In addition, using this polyclonal antibody they showed that there was an immunoreactive principle in the extract of calf thymus nuclei and its reactivity was lost by prior incubation with PARG or snake venom phosphodiesterase (pyrophosphatase), but not with DNase I, pancreatic RNase, nuclease P1, pronase E or 0.5 N NaOH treatment for 18 h at 37 °C. This was consistent with the character of in vitro synthesized PAR, indicating that there is PAR in calf thymus. They also showed that calf thymus contained PAR with various chain length (molecular size) and that PAR with shorter chain length reacted less efficiently to the antibody, as was expected with authentic PAR with shorter chain length. However, by normalizing the immunoreactivity of PAR with shorter chain length, the total amount of PAR in calf thymus was estimated as 740 pmol (0.4 μg)/g tissue or 37 pmol (0.02 μg)/mg DNA. Similar recovery of immunoreactivity was obtained when frozen calf thymus was homogenized directly in 5% trichloroacetic acid (TCA), without isolating nuclei, and processed similarly. This result suggested that there was no significant degradation or synthesis of PAR in vitro during the step of isolation of nuclei.
In 1979, Juarez-Salinas et al. treated cultured cells with 20% TCA, extracted PAR fraction and hydrolyzed PAR fraction with snake venom phosphodiesterase and alkaline phosphatase to isolate Ado-Rib (ribosyl adenosine), which is the dephosphorylated monomer form of PAR (Figure 2). Then they reacted this fraction with chloroacetaldehyde to make adenine residue to etheno adenine residue that is highly fluorescent. They separated these products with high performance liquid chromatography (HPLC) to show the presence of fluorescence-labeled etheno Ado-Rib, confirming the presence of PAR in vivo. Although the chain length distribution pattern of PAR could not be evaluated, this method could measure total amount of ADP-ribose residues constituting PAR quantitatively. In addition, they also showed that by treating the cultured cells with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), a DNA damaging agent, the PAR level increased dramatically but rapidly decreased [75] (Table 1).
Wielkens et al. prepared rabbit polyclonal antibody against Ado(P)-Rib-P (phosphoribosyl AMP, or pseudoADP-ribose), the specific component of PAR (Figure 2). In addition, they made [3H]PAR of high specific activity from [3H]NAD+ synthesized from [3H]ATP and nicotinamide mononucleotide using Ehrlich ascites tumor cell nuclei, which did not seem to contain significant amount of ATP. By combining radioimmunoassay and tracer [3H]PAR, they extracted PAR by first freeze-clamped normal rat liver. They reported the PAR level as 72.6–128.1 pmol/g rat liver tissue [69] (Table 1). Bredehorst et al. in the same research group reported the PAR level as 32 ± 9, 10 ± 2, 39 ± 5 pmol/mg DNA of neonatal (1 day), neonatal (17 days) and adult (>150 days) rat liver, respectively. They also reported the PAR level in hepatoma cells as 61 ± 11, 25 ± 3 and 60 ± 17 pmol/mg DNA for AH130, AH 7974 and Reuber H35 hepatomas, respectively [68] (Table 1).
In 1982, Juarez-Salinas et al. using their fluorescence labeling technique [75] reported that 14, 18, and 8.0 pmol of Ado-Rib/mg DNA from the liver, the kidney and the spleen of rats, respectively (Table 1). They also reported the content of Ado-Rib-Rib (derived from branch point, Figure 2) as 0.8–1.6% of total PAR [76]. Jacobson et al. reported PAR level as 12 pmol Ado-Rib/108 normal human diploid fibroblasts (CF-3) and it increased to 200 pmol/108 cells after UV irradiation [77]. They also measured PAR level in SV40-transformed Balb/3T3 fibroblasts and reported as 0.09 or 0.14 pmol Ado-Rib/106 cells and it increased by hyperthermia at 43 °C up to 5.25 pmol/106 cells after 8 h [78] (Table 1). A review on various methods for biochemical study of PAR metabolism in vitro and in vivo was published in 1995 [79]. Malanga et al. using polyacrylamide gel electrophoresis (PAGE), silver staining and computer-aided scanning densitometry reported PAR level in human keratinocyte cell line (HaKaT) as 0.554 pmol/106 cells (6.65 pmol/1.2 × 107 cells) and it increased to 11.1 pmol/106 cells (133 pmol/1.2 × 107 cells) after MNNG treatment [80] (Table 1). The interesting point is that although the total amount of PAR (the sum of ADP-ribose residues) is 20 times larger in the MNNG-treated cells, the chain length distribution pattern of PAR in the control (intact) cells seems not so different from that of MNNG-treated cells.
In 2008, Kinders et al. reported a sandwich ELISA of protein-bound PAR in tumor biopsies of human melanoma xenografts in mice after in vivo administration of PARP inhibitors (Table 1). This was a Phase 0 clinical trial of pharmacodynamic assay of PAR, leading to the current human clinical Phase 1 and 2 studies of PARP inhibitor in combination with anti-cancer drugs [81]. To invent a non-invasive procedure to monitor the effects of PARP inhibitor as a surrogate marker of the tumor in the body, Ji et al. using the above method of Kinders et al., reported PAR level in human peripheral blood mononuclear cells (PBMC) as 0.024 pmol/106 PBMC (13.17 ag/cell) from healthy volunteer and 0.028 pmol/106 PBMC (14.92 ag/cell) from patients with cancer. The intra- and inter-individual variation over 3 weeks in healthy volunteers was 0.008–0.198 pmol/106 PBMC (4.4–107.3 ag/cell) [46] (Table 1). The reason there was relatively big variation of intra- and inter-individual variation of PAR levels in PBMC, as compared to the later report by another researcher group [72] (Table 1), is not known at present. However, it might be necessary to compare precisely the conditions of preparation of PBMC samples before analyzing the actual PAR levels.
In 2013, Martello et al. reported PAR levels using a modern technique of stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) system (Table 1). They synthesized 13C,15N-labeled PAR from 13C,15N-labeled NAD+ and added to the sample as tracer for estimating the recovery of PAR during purification. They reported PAR levels as 0.03 pmol/106 PBMC and 0.05–0.1 pmol/106 HeLa cells. It is interesting that although there was little variation of basal PAR levels in PBMC from different donors, there was considerable interdonor variation of increased PAR levels after H2O2 stress. They also reported the PAR levels of mouse tissues as 21.5 ± 11.4, 10.3 ± 3.4, 7.5 ± 1.7, 6.2 ± 2.3, 5.9 ± 1.5, 4.6 ± 1.6 and 2.7 ± 1.0 pmol/mg DNA for the heart, the kidney, the liver, the thymus, the testis, the spleen, and the lung, respectively [72].
Up until now, for measurement of physiological low level of PAR, the importance of the sensitivity and the specificity have been greatly emphasized. However there have been less attention on the artificial synthesis or degradation of PAR during the extraction procedures. We recently reported a sandwich ELISA system to detect protein free PAR level with special attention to use TCA and not ordinary cell lysis buffer to extract PAR to avoid any artificial synthesis or degradation of PAR before ELISA. We used two kinds of antibodies against PAR, namely monoclonal antibody 10H [82] as catching antibody and rabbit polyclonal antibody against PAR, as was already reported [70]. To remove cross reacting or inhibiting materials as much as possible, in addition to DNase I and RNase A, nuclease P1 was used to digest oligonucleotides to 5′-mononucleotides. We also digested proteins in the sample to avoid any interference of the assay. In addition, to ensure the recovery of PAR, we did not use any columns such as DHB-Sepharose. With this method it became possible to measure the basal PAR level using 106 cells. The PAR levels were 0.039 pmol/106 HeLa cells (20.8 ag/cell), 0.038 pmol/106 HEK293T cells (20.5 ag/cell), and 0.002 pmol/106 HepG2 cells (1.0 ag/cell). The PAR level of HeLa cells increased 160 times to 6.39 pmol/106 HeLa cells (3460 ag/cell) after MNNG treatment [73]. The amount of PAR in HeLa cells is consistent with the value reported by Martello et al. [72] (Table 1). If the chain length distribution pattern of PAR did not change significantly in physiological condition, it is understandable that both ELISA system and LC-MS/MS system give similar results on the total amount of PAR. Yamashita et al. in the same research group reported PAR level in HeLa cells as 0.025 pmol/106 cells (13.3 ag/cell) and 0.074 pmol/106 cells (39.3 ag/cell) at 37 °C; 0.023 pmol/106 cells (12.5 ag/cell) at 33.5 °C; 0.085 pmol/106 cells (45.4 ag/cell) and 0.155 pmol/106 cells (82.9 ag/cell) at 40.5 °C. The PAR level in CHO-K1 cells was 0.063 pmol/106 cells (33.7 ag/cell) at 37 °C and 0.128 pmol/106 cells (68.2 ag/cell) at 40.5 °C (Table 1). With western blot analysis, γH2AX protein band was detected when HeLa cells or CHO-K1 cells were cultured at 40.5 °C and it further increased in the presence of 7 mM 3AB, a PARP inhibitor. Immunocytochemistry also showed similar results [83]. These results suggested the occurrence of double strand DNA breaks occurring at relatively mild shifted-up temperatures such as 40.5 °C, during which we might sometimes experience a microbial infection in daily life.
As described in the “Proposed functions of polyADP-ribosylation” section of this review, analysis of the amino acid modification sites of the acceptor proteins by polyADP-ribosylation are important, since they might directly modify the function of the acceptor proteins and also change the protein-protein interaction. For this purpose Chapman et al. hydrolyzed polyADP-ribosylated PARP1 with snake venom phosphodiesterase, and determined phospho-ribosylated amino acid residues using liquid chromatography-tandem mass spectrometry [84]. The quantification of the PAR attached to each acceptor amino acids will be expected direction in the future research. Since the structure of PAR with long chain length with many branches [52,53] is not studied well, it will be necessary not only to measure the amount of PAR, but also to analyze the role of the big PAR as a scaffold to support essential cellular functions. In this context, analysis of the proteomic approach to search protein motifs, which bind non-covalently to PAR, will give valuable information [56,85,86]. The information is important for analyzing the function of PAR in molecular levels. At the present time, we think our sandwich ELISA system will be useful in every research laboratory without expensive instrumentation, and is cost-effective. The catching monoclonal antibody (10H; anti poly(ADP-ribose) IgG3 kappa producing), which is necessary to coat the wells could be easily prepared by culturing 10H hybridoma cells [82], which is available from The RIKEN BioResource Research Center (BRC), a non-profit institution funded by the Japanese Government, under the code of (Cell No. RCB0705) (http://cell.brc.riken.jp/en/creditpay).
In addition it might be mentioned here that monoADP-ribosylation reaction is as important as polyADP-ribosylation in biological reaction. However, there is no suitable method to quantify the number of ADP-ribose molecules attached to each amino acids of the acceptor proteins. It is expected to develop such methods by applying recent techniques including mass spectrometry.

1.5. Prospect: In Vivo PAR Level to Understand More Biological Functions and Its Application for Clinic

PARP1 has been known to function in base excision repair (BER). When a single-strand break of DNA occurs, PARP1 immediately binds to the sites of DNA breaks and is activated to synthesize PAR attached to PARP1 itself (automodification), serving as a scaffold for recruiting BER enzymes. However, when PARP1 is inhibited by chemical inhibitor, single strand break persists and could make double strand break when replication fork passes through during DNA replication. These double strand breaks are usually repaired by homologous recombination controlled by BRCA1/2 gene(s). Therefore BRCA1- and BRCA2-mutated human breast cancer cells are subjected to killing by PARP inhibitors. This idea and the data were presented by 2 important papers (synthetic lethality) [87,88]. Since various anticancer drugs target DNA to make strand breaks to kill cancer cells, inhibition of DNA repair system in cancer cells is utmost effective. For this reason, combination of PARP inhibitors to DNA damaging anticancer drugs have also been studied in Phase 1 and 2 clinical trials [89,90,91,92]. It is interesting that cisplatin resistant non-small cell lung carcinoma cells frequently show hyperactivation of PARP than original tumor cells and the elevated PAR levels could be more accurate marker than the expression levels of PARP protein to predict the susceptibility of the cancer cells to PARP inhibitors [93].
Besides cancer, treatment of neurodegenerative diseases including Alzheimer disease and Parkinson’s disease becomes important in developed countries. When PAR is not degraded by mutation of degradation enzyme, PARG, neurodegenerative changes occurs in the brain and caused neurological symptoms in experimental animal model [26]. Also when the enzyme, TARG1, is mutated severe neurological symptoms appeared in the patient [12]. In addition, other diseases such as inflammation and immunological diseases are also considered to be possible targets of PARP inhibitors [94].

2. Conclusions

Since various biological functions have been proposed as described in the first part of this review, if we know more about quantitative view such as that on PAR level, we will be much closer to inventing effective methods to control or prevent diseases. Precise genomic information in addition to BRCA mutation might be required for classification of heterogeneous diseases to be treated. The important thing is that we will encourage more intimate information exchanges between bench and bedside.

Author Contributions

M.M. conceived the contents of this review; M.M., C.I., S.Y., Y.K., T.E., N.O., T.S. and M.Ta. had performed the cited experiments, which motivated to write this review; M.M., K.K., M.Ts. and M.Ta. collected the references and analyzed the data of sample preparation of the cited references on the in vivo level of PAR; M.M. wrote the paper.

Funding

The funding from the Ministry of Education, Culture, Sports, Science and Technology, from the Ministry of Health, Labour and Welfare, Japan, and from Nagahama Institute of Bio-Science and Technology, Japan, are greatly acknowledged.

Acknowledgments

We thank Kazuo Kamemura of Nagahama Institute of Bio-Science and Technology for critical reading of the manuscript. We also thank many collaborative researchers for support of our work.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Sugimura, T. Poly(adenosine diphosphate ribose). Prog. Nucleic Acid Res. Mol. Biol. 1973, 13, 127–151. [Google Scholar] [PubMed]
  2. Miwa, M.; Masutani, M. PolyADP-ribosylation and cancer. Cancer Sci. 2007, 98, 1528–1535. [Google Scholar] [CrossRef] [PubMed]
  3. Hottiger, M.O.; Hassa, P.O.; Lüscher, B.; Schüler, H.; Koch-Nolte, F. Toward a unified nomenclature for mammalian ADP-ribosyltransferases. Trends Biochem. Sci. 2010, 35, 208–219. [Google Scholar] [CrossRef] [PubMed]
  4. Chambon, P.; Weill, J.; Doly, J.; Strosser, M.; Mandel, P. On the formation of a novel adenylic compound by enzymatic extracts of liver nuclei. Biochem. Biophys Res. Commun. 1966, 25, 634–643. [Google Scholar] [CrossRef]
  5. Sugimura, T.; Fujimura, S.; Hasegawa, S.; Kawamura, Y. Polymerization of the adenosine 5′-diphosphate ribose moiety of NAD by rat liver nuclear enzyme. Biochim. Biophys. Acta 1967, 138, 438–441. [Google Scholar] [CrossRef]
  6. Nishizuka, Y.; Ueda, K.; Nakazawa, K.; Hayaishi, O. Studies on the polymer of adenosine diphosphate ribose. I. Enzymic formation from nicotinamide adenine dinuclotide in mammalian nuclei. J. Biol. Chem. 1967, 242, 3164–3171. [Google Scholar] [PubMed]
  7. Morrison, A.R.; Moss, J.; Stevens, L.A.; Evans, J.E.; Farrell, C.; Merithew, E.; Lambright, D.G.; Greiner, D.L.; Mordes, J.P.; Rossini, A.A.; et al. ART2, a T cell surface mono-ADP-ribosyltransferase, generates extracellular poly(ADP-ribose). J. Biol. Chem. 2006, 281, 33363–33372. [Google Scholar] [CrossRef] [PubMed]
  8. Miwa, M.; Sugimura, T. Splitting of the ribose-ribose linkage of poly(adenosine diphosphate-robose) by a calf thymus extract. J. Biol. Chem. 1971, 246, 6362–6364. [Google Scholar] [PubMed]
  9. Ueda, K.; Oka, J.; Narumiya, S.; Miyakawa, N.; Hayaishi, O. Poly ADP-ribose glycohydrolase from rat liver nuclei, a novel enzyme degrading the polymer. Biochem. Biophys Res. Commun. 1972, 46, 516–523. [Google Scholar] [CrossRef]
  10. Oka, S.; Kato, J.; Moss, J. Identification and characterization of a mammalian 39-kDa poly(ADP-ribose) glycohydrolase. J. Biol. Chem. 2006, 281, 705–713. [Google Scholar] [CrossRef] [PubMed]
  11. Oka, J.; Ueda, K.; Hayaishi, O.; Komura, H.; Nakanishi, K. ADP-ribosyl protein lyase. Purification, properties, and identification of the product. J. Biol. Chem. 1984, 259, 986–995. [Google Scholar] [PubMed]
  12. Sharifi, R.; Morra, R.; Appel, C.D.; Tallis, M.; Chioza, B.; Jankevicius, G.; Simpson, M.A.; Matic, I.; Ozkan, E.; Golia, B.; et al. Deficiency of terminal ADP-ribose protein glycohydrolase TARG1/C6orf130 in neurodegenerative disease. EMBO J. 2013, 32, 1225–1237. [Google Scholar] [CrossRef] [PubMed]
  13. Rosenthal, F.; Feijs, K.L.; Frugier, E.; Bonalli, M.; Forst, A.H.; Imhof, R.; Winkler, H.C.; Fischer, D.; Caflisch, A.; Hassa, P.O.; et al. Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases. Nat. Struct. Mol. Biol. 2013, 20, 502–507. [Google Scholar] [CrossRef] [PubMed]
  14. Jankevicius, G.; Hassler, M.; Golia, B.; Rybin, V.; Zacharias, M.; Timinszky, G.; Ladurner, A.G. A family of macrodomain proteins reverses cellular mono-ADP-ribosylation. Nat. Struct. Mol. Biol. 2013, 20, 508–514. [Google Scholar] [CrossRef] [PubMed]
  15. Poltronieri, P.; Cerekovic, N. Roles of nicotinamide adenine dinucleotide (NAD+) in biological systems. Challenges 2018, 9, 3. [Google Scholar] [CrossRef]
  16. Ueda, K.; Hayaishi, O. ADP-ribosylation. Annu. Rev. Biochem. 1985, 54, 73–100. [Google Scholar] [CrossRef] [PubMed]
  17. Althaus, F.R.; Richter, C. ADP-ribosylation of proteins. Enzymology and biological significance. Mol. Biol. Biochem. Biophys 1987, 37, 1–237. [Google Scholar] [PubMed]
  18. Schreiber, V.; Dantzer, F.; Ame, J.C.; de Murcia, G. Poly(ADP-ribose): Novel functions for an old molecule. Nat. Rev. Mol. Cell. Biol. 2006, 7, 517–528. [Google Scholar] [CrossRef] [PubMed]
  19. Kraus, W.L. PARPs and ADP-Ribosylation: 50 Years … and Counting. Mol. Cell 2015, 58, 902–910. [Google Scholar] [CrossRef] [PubMed]
  20. Hottiger, M.O. Nuclear ADP-Ribosylation and Its Role in Chromatin Plasticity, Cell Differentiation, and Epigenetics. Annu. Rev. Biochem. 2015, 4, 227–263. [Google Scholar] [CrossRef] [PubMed]
  21. Barkauskaite, E.; Jankevicius, G.; Ahel, I. Structures and Mechanisms of Enzymes Employed in the Synthesis and Degradation of PARP-Dependent Protein ADP-Ribosylation. Mol. Cell. 2015, 58, 935–946. [Google Scholar] [CrossRef] [PubMed]
  22. Crawford, K.; Bonfiglio, J.J.; Mikoč, A.; Matic, I.; Ahel, I. Specificity of reversible ADP-ribosylation and regulation of cellular processes. Crit. Rev. Biochem. Mol. Biol. 2018, 53, 64–82. [Google Scholar] [CrossRef] [PubMed]
  23. Wang, Z.Q.; Stingl, L.; Morrison, C.; Jantsch, M.; Los, M.; Schulze-Osthoff, K.; Wagner, E.F. PARP is important for genomic stability but dispensable in apoptosis. Genes Dev. 1997, 11, 2347–2358. [Google Scholar] [CrossRef] [PubMed]
  24. De Murcia, J.M.; Niedergang, C.; Trucco, C.; Ricoul, M.; Dutrillaux, B.; Mark, M.; Oliver, F.J.; Masson, M.; Dierich, A.; LeMeur, M.; et al. Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells. Proc. Natl. Acad. Sci. USA 1997, 94, 7303–7307. [Google Scholar] [CrossRef] [PubMed]
  25. Masutani, M.; Suzuki, H.; Kamada, N.; Watanabe, M.; Ueda, O.; Nozaki, T.; Jishage, K.; Watanabe, T.; Sugimoto, T.; Nakagama, H.; et al. Poly(ADP-ribose) polymerase gene disruption conferred mice resistant to streptozotocin-induced diabetes. Proc. Natl. Acad. Sci. USA 1999, 96, 2301–2304. [Google Scholar] [CrossRef] [PubMed]
  26. Hanai, S.; Kanai, M.; Ohashi, S.; Okamoto, K.; Yamada, M.; Takahashi, H.; Miwa, M. Loss of poly(ADP-ribose) glycohydrolase causes progressive neurodegeneration in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 2004, 101, 82–86. [Google Scholar] [CrossRef] [PubMed]
  27. Koh, D.W.; Lawler, A.M.; Poitras, M.F.; Sasaki, M.; Wattler, S.; Nehls, M.C.; Stöger, T.; Poirier, G.G.; Dawson, V.L.; Dawson, T.M. Failure to degrade poly(ADP-ribose) causes increased sensitivity to cytotoxicity and early embryonic lethality. Proc. Natl. Acad. Sci. USA 2004, 101, 17699–17704. [Google Scholar] [CrossRef] [PubMed]
  28. Durkacz, B.W.; Omidiji, O.; Gray, D.A.; Shall, S. (ADP-ribose)n participates in DNA excision repair. Nature 1980, 283, 593–596. [Google Scholar] [CrossRef] [PubMed]
  29. Smulson, M.; Stark, P.; Gazzoli, M.; Roberts, J. Release of template restriction for DNA synthesis by poly ADP(ribose) polymerase during the HeLa cell cycle. Exp. Cell Res. 1975, 90, 175–182. [Google Scholar] [CrossRef]
  30. Simbulan-Rosenthal, C.M.; Rosenthal, D.S.; Smulson, M.E. Purification and characterization of poly(ADP-ribosyl)ated DNA replication/repair complexes. Methods Mol. Biol. 2011, 780, 165–190. [Google Scholar] [PubMed]
  31. Tulin, A.; Spradling, A. Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci. Science 2003, 299, 560–562. [Google Scholar] [CrossRef] [PubMed]
  32. Kraus, W.L. Transcriptional control by PARP-1: Chromatin modulation, enhancer-binding, coregulation, and insulation. Curr. Opin. Cell Biol. 2008, 20, 294–302. [Google Scholar] [CrossRef] [PubMed]
  33. Aubin, R.J.; Fréchette, A.; de Murcia, G.; Mandel, P.; Lord, A.; Grondin, G.; Poirier, G.G. Correlation between endogenous nucleosomal hyper(ADP-ribosyl)ation of histone H1 and the induction of chromatin relaxation. EMBO J. 1983, 2, 1685–1693. [Google Scholar] [PubMed]
  34. Realini, C.A.; Althaus, F.R. Histone shuttling by poly(ADP-ribosylation). J. Biol. Chem. 1992, 267, 18858–18865. [Google Scholar] [PubMed]
  35. Utakoji, T.; Hosoda, K.; Umezawa, K.; Sawamura, M.; Matsushima, T.; Miwa, M.; Sugimura, T. Induction of sister chromatid exchanges by nicotinamide in Chinese hamster lung fibroblasts and human lymphoblastoid cells. Biochem. Biophys. Res. Commun. 1979, 90, 1147–1152. [Google Scholar] [CrossRef]
  36. Oikawa, A.; Tohda, H.; Kanai, M.; Miwa, M.; Sugimura, T. Inhibitors of poly(adenosine diphosphate ribose) polymerase induce sister chromatid exchanges. Biochem. Biophys. Res. Commun. 1980, 97, 1311–1316. [Google Scholar] [CrossRef]
  37. Kanai, M.; Tong, W.M.; Sugihara, E.; Wang, Z.Q.; Fukasawa, K.; Miwa, M. Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function. Mol. Cell. Biol. 2003, 23, 2451–2462. [Google Scholar] [CrossRef] [PubMed]
  38. Smith, S.; Giriat, I.; Schmitt, A.; de Lange, T. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres. Science 1998, 282, 1484–1487. [Google Scholar] [CrossRef] [PubMed]
  39. Cohen-Armon, M.; Visochek, L.; Rozensal, D.; Kalal, A.; Geistrikh, I.; Klein, R.; Bendetz-Nezer, S.; Yao, Z.; Seger, R. DNA-independent PARP-1 activation by phosphorylated ERK2 increases Elk1 activity: A link to histone acetylation. Mol. Cell 2007, 25, 297–308. [Google Scholar] [CrossRef] [PubMed]
  40. Kanai, M.; Hanashiro, K.; Kim, S.H.; Hanai, S.; Boulares, A.H.; Miwa, M.; Fukasawa, K. Inhibition of Crm1-p53 interaction and nuclear export of p53 by poly(ADP-ribosyl)ation. Nat. Cell Biol. 2007, 9, 1175–1183. [Google Scholar] [CrossRef] [PubMed]
  41. Stilmann, M.; Hinz, M.; Arslan, S.C.; Zimmer, A.; Schreiber, V.; Scheidereit, C. A nuclear poly(ADP-ribose)-dependent signalosome confers DNA damage-induced IkappaB kinase activation. Mol. Cell 2009, 36, 365–378. [Google Scholar] [CrossRef] [PubMed]
  42. Wang, Y.; Kim, N.S.; Haince, J.F.; Kang, H.C.; David, K.K.; Andrabi, S.A.; Poirier, G.G.; Dawson, V.L.; Dawson, T.M. Poly(ADP-ribose) (PAR) binding to apoptosis-inducing factor is critical for PAR polymerase-1-dependent cell death (parthanatos). Sci. Signal. 2011, 4, ra20. [Google Scholar] [CrossRef] [PubMed]
  43. Masutani, M.; Fujimori, H. Poly(ADP-ribosyl)ation in carcinogenesis. Mol. Asp. Med. 2013, 34, 1202–1216. [Google Scholar] [CrossRef] [PubMed]
  44. Ji, Y.; Thomas, C.; Tulin, N.; Lodhi, N.; Boamah, E.; Kolenko, V.; Tulin, A.V. Charon Mediates Immune Deficiency-Driven PARP-1-Dependent Immune Responses in Drosophila. J. Immunol. 2016, 197, 2382–2389. [Google Scholar] [CrossRef] [PubMed]
  45. Szabó, C.; Dawson, V.L. Role of poly(ADP-ribose) synthetase in inflammation and ischaemia-reperfusion. Trends Pharmacol. Sci. 1998, 19, 287–298. [Google Scholar] [CrossRef]
  46. Ji, J.; Kinders, R.J.; Zhang, Y.; Rubinstein, L.; Kummar, S.; Parchment, R.E.; Tomaszewski, J.E.; Doroshow, J.H. Modeling pharmacodynamic response to the poly(ADP-Ribose) polymerase inhibitor ABT-888 in human peripheral blood mononuclear cells. PLoS ONE 2011, 6. [Google Scholar] [CrossRef] [PubMed]
  47. Yoshihara, K.; Hashida, T.; Yoshihara, H.; Tanaka, Y.; Ohgushi, H. Enzyme-bound early product of purified poly(ADP-ribose) polymerase. Biochem. Biophys. Res. Commun. 1977, 78, 1281–1288. [Google Scholar] [CrossRef]
  48. Ogata, N.; Ueda, K.; Hayaishi, O. ADP-ribosylation of histone H2B. Identification of glutamic acid residue 2 as the modification site. J. Biol. Chem. 1980, 255, 7610–7615. [Google Scholar] [PubMed]
  49. Ogata, N.; Ueda, K.; Kagamiyama, H.; Hayaishi, O. ADP-ribosylation of histone H1. Identification of glutamic acid residues 2, 14, and the COOH-terminal lysine residue as modification sites. J. Biol. Chem. 1980, 255, 7616–7620. [Google Scholar] [PubMed]
  50. Suzuki, H.; Quesada, P.; Farina, B.; Leone, E. In vitro poly(ADP-ribosyl)ation of seminal ribonuclease. J. Biol. Chem. 1986, 261, 6048–6055. [Google Scholar] [PubMed]
  51. Wesierska-Gadek, J.; Schmid, G.; Cerni, C. ADP-ribosylation of wild-type p53 in vitro: Binding of p53 protein to specific p53 consensus sequence prevents its modification. Biochem. Biophys. Res. Commun. 1996, 224, 96–102. [Google Scholar] [CrossRef] [PubMed]
  52. Miwa, M.; Saikawa, N.; Yamaizumi, Z.; Nishimura, S.; Sugimura, T. Structure of poly(adenosine diphosphate ribose): Identification of 2′-[1′′-ribosyl-2′′-(or 3′′-)(1′′′-ribosyl)]adenosine-5′,5′′,5′′′-tris(phosphate) as a branch linkage. Proc. Natl. Acad. Sci. USA 1979, 76, 595–599. [Google Scholar] [CrossRef] [PubMed]
  53. De Murcia, G.; Jongstra-Bilen, J.; Ittel, M.E.; Mandel, P.; Delain, E. Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: Electron microscopic visualization. EMBO J. 1983, 2, 543–548. [Google Scholar] [PubMed]
  54. Hayashi, K.; Tanaka, M.; Shimada, T.; Miwa, M.; Sugimura, T. Size and shape of poly(ADP-ribose): Examination by gel filtration, gel electrophoresis and electron microscopy. Biochem. Biophys. Res. Commun. 1983, 112, 102–107. [Google Scholar] [CrossRef]
  55. Panzeter, P.L.; Realini, C.A.; Althaus, F.R. Noncovalent interactions of poly(adenosine diphosphate ribose) with histones. Biochemistry 1992, 31, 1379–1385. [Google Scholar] [CrossRef] [PubMed]
  56. Malanga, M.; Althaus, F.R. Noncovalent protein interaction with poly(ADP-ribose). Methods Mol. Biol. 2011, 780, 67–82. [Google Scholar] [PubMed]
  57. Li, M.; Yu, X. Function of BRCA1 in the DNA damage response is mediated by ADP-ribosylation. Cancer Cell 2013, 23, 693–704. [Google Scholar] [CrossRef] [PubMed]
  58. Zaja, R.; Mikoč, A.; Barkauskaite, E.; Ahel, I. Molecular Insights into Poly(ADP-ribose) Recognition and Processing. Biomolecules 2012, 3, 1–17. [Google Scholar] [CrossRef] [PubMed]
  59. Barkauskaite, E.; Jankevicius, G.; Ladurner, A.G.; Ahel, I.; Timinszky, G. The recognition and removal of cellular poly(ADP-ribose) signals. FEBS J. 2013, 280, 3491–3507. [Google Scholar] [CrossRef] [PubMed]
  60. Daniels, C.M.; Ong, S.E.; Leung, A.K. The Promise of Proteomics for the Study of ADP-Ribosylation. Mol. Cell 2015, 58, 911–924. [Google Scholar] [CrossRef] [PubMed]
  61. Pleschke, J.M.; Kleczkowska, H.E.; Strohm, M.; Althaus, F.R. Poly(ADP-ribose) binds to specific domains in DNA damage checkpoint proteins. J. Biol. Chem. 2000, 275, 40974–40980. [Google Scholar] [CrossRef] [PubMed]
  62. Miwa, M.; Ida, C.; Yamashita, S.; Tanaka, M.; Fujisawa, J. Poly(ADP-ribose): Structure, Physicochemical Properties and Quantification In Vivo, with Special Reference to Poly(ADP-ribose) Binding Protein Modules. Curr. Protein Pept. Sci. 2016, 17, 683–692. [Google Scholar] [CrossRef] [PubMed]
  63. Doly, J.; Mandel, P. Mise en évidence de la biosynthèse in vivo d’un polymère composé, le polyadénosine diphosphoribose dans les noyaux de foie de poulet. C. R. Acad. Sci. 1967, 264, 2687–2690. [Google Scholar]
  64. Ueda, K.; Omachi, A.; Kawaichi, M.; Hayaishi, O. Natural occurrence of poly(ADP-ribosyl) histones in rat liver. Proc. Natl. Acad. Sci. USA 1975, 72, 205–209. [Google Scholar] [CrossRef] [PubMed]
  65. Kanai, Y.; Kawaminami, Y.; Miwa, M.; Matsushima, T.; Sugimura, T. Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus. Nature 1977, 265, 175–177. [Google Scholar] [CrossRef] [PubMed]
  66. Smith, J.A.; Stocken, L.A. Identification of poly (ADP-ribose) covalently bound to histone F1 in vivo. Biochem. Biophys. Res. Commun. 1973, 54, 297–300. [Google Scholar] [CrossRef]
  67. Stone, P.R.; Bredehorst, R.; Kittler, M.; Lengyel, H.; Hilz, H. Quantitative determination of poly(adenosine diphosphate ribose) in different hepatic tissues by an isotope dilution procedure. Hoppe Seylers Z. Physiol. Chem. 1976, 357, 51–56. [Google Scholar] [CrossRef] [PubMed]
  68. Bredehorst, R.; Wielckens, K.; Adamietz, P.; Steinhagen-Thiessen, E.; Hilz, H. Mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation of proteins in developing liver and in hepatomas: Relation of conjugate subfractions to metabolic competence and proliferation rates. Eur. J. Biochem. 1981, 120, 267–274. [Google Scholar] [CrossRef] [PubMed]
  69. Wielckens, K.; Bredehorst, R.; Adamietz, P.; Hilz, H. Protein-bound polymeric and monomeric ADP-ribose residues in hepatic tissues. Comparative analyses using a new procedure for the quantification of poly(ADP-ribose). Eur. J. Biochem. 1981, 117, 69–74. [Google Scholar] [CrossRef] [PubMed]
  70. Kanai, Y.; Miwa, M.; Matsushima, T.; Sugimura, T. Anti-poly(ADP-ribose) antibody and its specificity. J. Biochem. 1975, 77, 5–6. [Google Scholar] [CrossRef]
  71. Kidwell, W.R.; Mage, M.G. Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cells. Biochemistry 1976, 15, 1213–1217. [Google Scholar] [CrossRef] [PubMed]
  72. Martello, R.; Mangerich, A.; Sass, S.; Dedon, P.C.; Bürkle, A. Quantification of cellular poly(ADP-ribosyl)ation by stable isotope dilution mass spectrometry reveals tissue- and drug-dependent stress response dynamics. ACS Chem. Biol. 2013, 8, 1567–1575. [Google Scholar] [CrossRef] [PubMed]
  73. Ida, C.; Yamashita, S.; Tsukada, M.; Sato, T.; Eguchi, T.; Tanaka, M.; Ogata, S.; Fujii, T.; Nishi, Y.; Ikegami, S.; et al. An enzyme-linked immunosorbent assay-based system for determining the physiological level of poly(ADP-ribose) in cultured cells. Anal. Biochem. 2016, 494, 76–81. [Google Scholar] [CrossRef] [PubMed]
  74. Sakura, H.; Miwa, M.; Tanaka, M.; Kanai, Y.; Shimada, T.; Matsushima, T.; Sugimura, T. Natural occurence of a biopolymer, poly (adenosine diphosphate ribose). Nucleic Acids Res. 1977, 4, 2903–2915. [Google Scholar] [CrossRef] [PubMed]
  75. Juarez-Salinas, H.; Sims, J.L.; Jacobson, M.K. Poly(ADP-ribose) levels in carcinogen-treated cells. Nature 1979, 282, 740–741. [Google Scholar] [CrossRef] [PubMed]
  76. Juarez-Salinas, H.; Levi, V.; Jacobson, E.L.; Jacobson, M.K. Poly(ADP-ribose) has a branched structure in vivo. J. Biol. Chem. 1982, 257, 607–609. [Google Scholar] [PubMed]
  77. Jacobson, E.L.; Antol, K.M.; Juarez-Salinas, H.; Jacobson, M.K. Poly(ADP-ribose) metabolism in ultraviolet irradiated human fibroblasts. J. Biol. Chem. 1983, 258, 103–107. [Google Scholar] [PubMed]
  78. Juarez-Salinas, H.; Duran-Torres, G.; Jacobson, M.K. Alteration of poly(ADP-ribose) metabolism by hyperthermia. Biochem. Biophys. Res. Commun. 1984, 122, 1381–1388. [Google Scholar] [CrossRef]
  79. Shah, G.M.; Poirier, D.; Duchaine, C.G.; Desnoyers, S.; Lagueux, J.; Verreault, A.; Hoflack, J.C.; Kirkland, J.B.; Poirier, G.G. Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo. Anal. Biochem. 1995, 227, 1–13. [Google Scholar] [CrossRef] [PubMed]
  80. Malanga, M.; Bachmann, S.; Panzeter, P.L.; Zweifel, B.; Althaus, F.R. Poly(ADP-ribose) quantification at the femtomole level in mammalian cells. Anal. Biochem. 1995, 228, 245–251. [Google Scholar] [CrossRef] [PubMed]
  81. Kinders, R.J.; Hollingshead, M.; Khin, S.; Rubinstein, L.; Tomaszewski, J.E.; Doroshow, J.H.; Parchment, R.E. National Cancer Institute Phase 0 Clinical Trials Team. Preclinical modeling of a phase 0 clinical trial: Qualification of a pharmacodynamic assay of poly (ADP-ribose) polymerase in tumor biopsies of mouse xenografts. Clin. Cancer Res. 2008, 14, 6877–6885. [Google Scholar] [CrossRef] [PubMed]
  82. Kawamitsu, H.; Hoshino, H.; Okada, H.; Miwa, M.; Momoi, H.; Sugimura, T. Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures. Biochemistry 1984, 23, 3771–3777. [Google Scholar] [CrossRef] [PubMed]
  83. Yamashita, S.; Tanaka, M.; Sato, T.; Ida, C.; Ohta, N.; Hamada, T.; Uetsuki, T.; Nishi, Y.; Moss, J.; Miwa, M. Effect of mild temperature shift on poly(ADP-ribose) and γH2AX levels in cultured cells. Biochem. Biophys. Res. Commun. 2016, 476, 594–599. [Google Scholar] [CrossRef] [PubMed]
  84. Chapman, J.D.; Gagné, J.P.; Poirier, G.G.; Goodlett, D.R. Mapping PARP-1 auto-ADP-ribosylation sites by liquid chromatography-tandem mass spectrometry. J. Proteome Res. 2013, 12, 1868–1880. [Google Scholar] [CrossRef] [PubMed]
  85. Malanga, M.; Althaus, F.R. The role of poly(ADP-ribose) in the DNA damage signaling network. Biochem. Cell Biol. 2005, 83, 354–364. [Google Scholar] [CrossRef] [PubMed]
  86. Gagné, J.P.; Isabelle, M.; Lo, K.S.; Bourassa, S.; Hendzel, M.J.; Dawson, V.L.; Dawson, T.M.; Poirier, G.G. Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. Nucleic Acids Res. 2008, 36, 6959–6976. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  87. Bryant, H.E.; Schultz, N.; Thomas, H.D.; Parker, K.M.; Flower, D.; Lopez, E.; Kyle, S.; Meuth, M.; Curtin, N.J.; Helleday, T. Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase. Nature 2005, 434, 913–917. [Google Scholar] [CrossRef] [PubMed]
  88. Farmer, H.; McCabe, N.; Lord, C.J.; Tutt, A.N.; Johnson, D.A.; Richardson, T.B.; Santarosa, M.; Dillon, K.J.; Hickson, I.; Knights, C.; et al. Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy. Nature 2005, 434, 917–921. [Google Scholar] [CrossRef] [PubMed]
  89. Curtin, N.J.; Szabo, C. Therapeutic applications of PARP inhibitors: Anticancer therapy and beyond. Mol. Asp. Med. 2013, 34, 1217–1256. [Google Scholar] [CrossRef] [PubMed]
  90. Kummar, S.; Ji, J.; Morgan, R.; Lenz, H.J.; Puhalla, S.L.; Belani, C.P.; Gandara, D.R.; Allen, D.; Kiesel, B.; Beumer, J.H.; et al. A phase I study of veliparib in combination with metronomic cyclophosphamide in adults with refractory solid tumors and lymphomas. Clin. Cancer Res. 2012, 18, 1726–1734. [Google Scholar] [CrossRef] [PubMed]
  91. Kummar, S.; Wade, J.L.; Oza, A.M.; Sullivan, D.; Chen, A.P.; Gandara, D.R.; Ji, J.; Kinders, R.J.; Wang, L.; Allen, D.; et al. Randomized phase II trial of cyclophosphamide and the oral poly (ADP-ribose) polymerase inhibitor veliparib in patients with recurrent, advanced triple-negative breast cancer. Investig. New Drugs 2016, 34, 355–363. [Google Scholar] [CrossRef] [PubMed]
  92. Drew, Y.; Ledermann, J.; Hall, G.; Rea, D.; Glasspool, R.; Highley, M.; Jayson, G.; Sludden, J.; Murray, J.; Jamieson, D.; et al. Phase 2 multicentre trial investigating intermittent and continuous dosing schedules of the poly(ADP-ribose) polymerase inhibitor rucaparib in germline BRCA mutation carriers with advanced ovarian and breast cancer. Br. J. Cancer 2016, 114, 723–730. [Google Scholar] [CrossRef] [PubMed]
  93. Michels, J.; Vitale, I.; Galluzzi, L.; Adam, J.; Olaussen, K.A.; Kepp, O.; Senovilla, L.; Talhaoui, I.; Guegan, J.; Enot, D.P.; et al. Cisplatin resistance associated with PARP hyperactivation. Cancer Res. 2013, 73, 2271–2280. [Google Scholar] [CrossRef] [PubMed]
  94. Berger, N.A.; Besson, V.C.; Boulares, A.H.; Bürkle, A.; Chiarugi, A.; Clark, R.S.; Curtin, N.J.; Cuzzocrea, S.; Dawson, T.M.; Dawson, V.L.; et al. Opportunities for the repurposing of PARP inhibitors for the therapy of non-oncological diseases. Br. J. Pharmacol. 2018, 175, 192–222. [Google Scholar] [CrossRef] [PubMed]
Challenges 09 00023 g001 550
Figure 1. PolyADP-ribosylation reaction. ARH3: ADP-ribosylhydrolase 3; ART2: ADP-ribosyltransferase 2; Macro: macrodomain-containing proteins; Na: nicotinic acid; Nam: nicotinamide; NaMN: nicotinic acid mononucleotide; NMN: nicotinamide mononucleotide; NaAD: nicotinic acid adenine dinucleotide; PAR: poly(ADP-ribose); PARG: poly(ADP-ribose) glycohydrolase; PARPs: poly(ADP-ribose) polymerases; TARG1: Terminal ADP-ribose protein glycohydrolase 1.
Figure 1. PolyADP-ribosylation reaction. ARH3: ADP-ribosylhydrolase 3; ART2: ADP-ribosyltransferase 2; Macro: macrodomain-containing proteins; Na: nicotinic acid; Nam: nicotinamide; NaMN: nicotinic acid mononucleotide; NMN: nicotinamide mononucleotide; NaAD: nicotinic acid adenine dinucleotide; PAR: poly(ADP-ribose); PARG: poly(ADP-ribose) glycohydrolase; PARPs: poly(ADP-ribose) polymerases; TARG1: Terminal ADP-ribose protein glycohydrolase 1.
Challenges 09 00023 g001
Challenges 09 00023 g002 550
Figure 2. Specific degradation products of polyADP-ribosylation.
Figure 2. Specific degradation products of polyADP-ribosylation.
Challenges 09 00023 g002
Table
Table 1. In vivo level of PAR assayed by various methods.
Table 1. In vivo level of PAR assayed by various methods.
Name of MethodsTargetReagentsTissues or CellsFixationExtraction, PurificationSensitivity *Level of PAR *Comments by AuthorsReferences
Chemical analysisHistone F1 Rat liver nucleiHistone F1 extracted with 5% HClO4 and precipitated with 20% TCADEAE-cellulose column chromatography to separate from non-histone proteins 0.89–1.73 nmol/mg F1 histone protein Smith and Stocken (1973) [66]
Isotope dilutionAdo(P)-Rib-P[3H]PAR as indicator, prepared using sonicated Ehrlich carcinoma cell lysate as enzyme sourceRat liver (adult and neonatal), Zajdela hepatomaFreeze-clamped (rat liver)Homogenized in 0.25 N KOH, sonicated, 1 M NH2OH; venom PDE; anion exchange column chromatography; paper chromatography; phosphatase; paper chromatography 5.59 (adult), 6.32 (neonatal), 1.24 (hepatoma) nmol/mg DNA Stone et al. (1976) [67]
RIAPAR[14C]PAR, polyclonal antibody against PARHeLa cells0.1 N NaOH, 2 h, 37 °C; 4 °C overnightDNase I, micrococcal nuclease; Pronase; phenol extraction 3.7 pmol/106 HeLa cells; small peak of PAR at S phase and large peak at G2 phase Kidwell and Mage (1976) [71]
RIAPAR[14C]PAR, polyclonal antibody against PARCalf tissuesFrozen after slaughterhouseHomogenized to isolate nuclei and ethanol precipitation; or homogenized in 5% TCA without isolating nuclei; Pronase; phenol extraction; DNase I, RNase, nuclease P1; Pronase; phenol extraction; hydroxyapatite column chromatography10 pmol of PAR37 pmol/mg DNA or 740 pmol/g calf thymus Chain length distribution shown with hydroxyapatite column chromatographySakura et al. (1977) [74]
Fluorescence methodEtheno Ado-RibFluorescent labeling of Ado-RibSV40-transformed 3T3 cells20% TCA0.1 M potassium phosphate (pH 5) in 6 M guanidine hydrochloride, sonication; dihydroxyboryl-Sepharose column chromatography; alkaline phosphatase and venom PDE; chloroacetaldehyde; HPLC5 pmol of Ado-Rib0.05 pmol/106 SV40 virus-transformed 3T3 cells, increased to 7.50 pmol/106 cells after 20 min of MNNG Juarez-Salinas et al. (1979) [75]
RIA: Method AAdo(P)-Rib-P[3H]PAR as tracer prepared using Ehrlich carcinoma cell nuclei, [3H]Ado(P)-Rib-P; antibody against Ado(P)-Rib-PRat liver (adult)Freeze-clamped and frozenHomogenized in 20% TCA; dissolved in 6 M guanidine hydrochloride and morpholine buffer; 0.3 M NaOH, 1 h, 56 °C; boronate column chromatography; venom PDE; HPLC1 pmol Ado(P)-Rib-P88.6 ± 20.7 and 72.6 pmol/g tissue Wielkens et al. (1981) [69]
RIA: Method BAdo(P)-Rib-PSame as aboveSame as aboveSame as aboveHomogenized in 20% TCA; dissolved in cold water; 0.33 M NaOH, 3 h, 56 °C; alkaline phosphatase; proteinase K; heat inactivation; venom PDE; 5% TCA; anion exchange column chromatography, HPLC1 pmol Ado(P)-Rib-P89.0 ± 10.7 and 128.1 ± 7.3 pmol/g tissue Wielkens et al. (1981) [69]
RIAAdo(P)-Rib-PRIA Method A (Wielkens et al. 1981) [69]Rat liver (adult and neonatal) and hepatomaFreeze-clamped and frozen (rat liver) or washed and frozen in liquid nitrogen (hepatoma cells)RIA Method A (Wielkens et al., 1981) [69]1 pmol Ado(P)-Rib-P32 ± 9, 10 ± 2, 39 ± 5 pmol/mg DNA of neonatal (1 day), neonatal (17 days) and adult (>150 days) rat liver, respectively. 61 ± 11, 25 ± 3 and 60 ± 17 pmol/mg DNA for AH130, AH 7974 and Reuber H35 hepatomas, respectively Bredehorst et al. (1981) [68]
Fluorescence methodEtheno Ado-Rib and etheno Ado-Rib-RibFluorescent labeling of specific degradation product, Ado-Rib and Ado-Rib-RibRat liver, kidney and spleen20% TCAJuarez-Salinas et al. (1979) [75] 14, 18, and 8.0 pmol of Ado-Rib/mg DNA of liver, kidney and spleen of rat, respectively; Ado-Rib-Rib constituted 0.8-1.6% of total PAR Juarez-Salinas et al. (1982) [76]
Fluorescence methodEtheno Ado-RibFluorescent labeling of specific degradation product, Ado-RibNormal human diploid fibroblasts (CF-3)20% TCAMOPS/KOH (pH 8.8) in 6 M guanidine hydrochloride, sonication; dihydroxyboryl BioRex 70 resin; alkaline phosphatase and venom PDE; fluorescent etheno derivatization; dihydroxyboronate column chromatography; HPLC 0.12 pmol Ado-Rib/106 cells; increased to 2.0 pmol/106 cells after UV Jacobson et al. (1983) [77]
Fluorescence methodEtheno Ado-RibFluorescent labeling of Ado-RibSV40-transformed Balb/3T3 fibroblasts20% TCAJacobson et al. (1983) [77] 0.09 or 0.14 pmol Ado-Rib/106 cells: increased by hyperthermia at 43 °C up to 5.25 pmol/106 cells after 8 hNo significant DNA strand breaks upon alkaline sucrose gradient centrifugationJuarez-Salinas et al. (1984) [78]
PAGE, silver staining and computer-aided scanning densitometryPARPAR standardHuman keratinocyte cell line (HaKaT)20% TCAProteinase K (DNase I, RNase); 1 M KOH, 37 °C 2 h; dihydroxyboronate column chromatography; 20% PAGE; silver staining; scanned with computing densitometer (ImageQuant 3.15 software) 0.55 pmol/106 cells; MNNG treatment increased to 11.1 pmol/106 cellsChain length distribution shown with PAGEMalanga et al. (1995) [80]
Sandwich ELISAProtein-bound PARPAR standard; mouse monoclonal (10H) and rabbit polyclonal antibody against PARXenograft tumors of human melanoma cell lines (A375 and Colo829)Fresh frozenSonicated in lysis buffer (Biosource) with protease inhibitors; 1% SDS; boiled 5 min; supernatant 5584 units PAR (large tumors); 4146 units PAR (small tumors) [All units are pg PAR/mL per 100 mg protein]; Higher amount of protein and DNA caused interferenceProtein and DNA were not digested. Protein concentration should be diluted to 0.1–1 mg/mL before assay. Effect of dose of PARP inhibitor and time course of PAR levels in xenograft were presentedKinders et al. (2008) [81]
Sandwich ELISAProtein-bound PARPAR standard; mouse monoclonal (10H) and rabbit polyclonal antibody against PARHuman PBMCFresh frozenSuspended in cell extraction buffer (Invitrogen) with protease inhibitors; 30 min on ice; 1% SDS; boiled 5 min; supernatant 0.024 pmol/106 PBMC (healthy volunteer); 0.028 pmol/106 PBMC (patients with cancer); 0.008-0.198 pmol/106 PBMC (intra- and inter-individual variation over 3 weeks in healthy volunteers)Protein and DNA were not digestedJi et al. (2011) [46]
Stable isotope dilution LC-MS/MSPAR13C,15N-labeled PARPBMC, COPFS cells, HeLa cells, mouse tissues20% TCA0.5 M KOH, 37 °C, 45 min; 13C, 15N labelled PAR added; DNase I, RNase A; proteinase K; solid phase extraction of PAR; venom PDE, alkaline phosphatase; HPLC; mass spectrometry50 fmol of Ado-Rib0.03 pmol/106 PBMC: 0.05-0.1 pmol/106 HeLa cellsConsiderable heterogeneity in the stress-induced PARylation response in human populationMartello et al. (2013) [72]
Sandwich ELISAPARPAR standard; mouse monoclonal (10H) and rabbit polyclonal antibody against PARHeLa cells; HEK293T cells; HepG2 cells20% TCASonication; 0.1 N NaOH, 37 °C 1 h; DNase I, RNase A, Nuclease P1, Proteinase K; 15 fmol of PAR0.039 pmol/106 HeLa cells; 0.038 pmol/106 HEK293T cells; 0.002 pmol/106 HepG2 cells; increased to 6.49 pmol/106 HeLa cells after MNNG.Big difference of PAR level depending the isolation procedures and cell typesIda et al. (2016) [73]
Sandwich ELISAPARPAR standard; mouse monoclonal (10H) and rabbit polyclonal antibody against PARHeLa cells, CHO-K1 cells20% TCAIda et al. (2016) [73]15 fmol of PAR0.025 and 0.074 pmol/106 HeLa cells at 37 °C; 0.023 pmol/106 cells at 33.5 °C; 0.085 and 0.155 pmol/106 cells at 40.5 °C; 0.063 pmol/106 CHO-K1 cells at 37 °C; 0.128 pmol/106 cells at 40.5 °CPhosphorylation of histone H2AX was found at 40.5 °C and further increased by PARP inhibitor.Yamashita et al. (2016) [83]
Ado(P)-Rib-P: phosphoribosyl AMP; Ado-Rib: ribosyl adenosine; Ado-Rib-Rib: ribosyl-ribosyl adenosine; ag: attogram; amol: attomol; DEAE: diethylaminoethyl; ELISA: enzyme-linked immunosorbent assay; HPLC: high performance liquid chromatography; LC-MS/MS: liquid chromatography-tandem mass spectrometry; MNNG: N-methyl-N′-nitro-N-nitrosoguanidine; MOPS: 3-(N-morpholino)propanesulfonic acid; PAGE: polyacrylamide gel electrophoresis; PAR: poly(ADP-ribose); PARylation: polyADP-ribosylation; PBMC: peripheral blood mononuclear cells; PDE: phosphodiesterase; RIA: radioimmunoassay; SDS: sodium dodecyl sulfate; TCA: trichloroacetic acid; UV: ultraviolet. * The amount of PAR was expressed as ADP-ribose residues and calculated to moles (mol)/mg DNA or 106 cells, taking into consideration of the molecular weight of internal ADP-ribose residue as 541.

Share and Cite

MDPI and ACS Style

Miwa, M.; Ida, C.; Yamashita, S.; Kouyama, K.; Kuroda, Y.; Eguchi, T.; Ohta, N.; Sato, T.; Tsuda, M.; Tanaka, M. In Vivo Level of Poly(ADP-ribose). Challenges 2018, 9, 23. https://doi.org/10.3390/challe9010023

AMA Style

Miwa M, Ida C, Yamashita S, Kouyama K, Kuroda Y, Eguchi T, Ohta N, Sato T, Tsuda M, Tanaka M. In Vivo Level of Poly(ADP-ribose). Challenges. 2018; 9(1):23. https://doi.org/10.3390/challe9010023

Chicago/Turabian Style

Miwa, Masanao, Chieri Ida, Sachiko Yamashita, Kenichi Kouyama, Yasuhito Kuroda, Takayuki Eguchi, Narumi Ohta, Teruaki Sato, Masataka Tsuda, and Masakazu Tanaka. 2018. "In Vivo Level of Poly(ADP-ribose)" Challenges 9, no. 1: 23. https://doi.org/10.3390/challe9010023

APA Style

Miwa, M., Ida, C., Yamashita, S., Kouyama, K., Kuroda, Y., Eguchi, T., Ohta, N., Sato, T., Tsuda, M., & Tanaka, M. (2018). In Vivo Level of Poly(ADP-ribose). Challenges, 9(1), 23. https://doi.org/10.3390/challe9010023

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop