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Article

Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile

by
Mario Quezada-Aguiluz
1,2,3,4,
Alejandro Aguayo-Reyes
1,2,5,6,
Cinthia Carrasco
1,
Daniela Mejías
1,
Pamela Saavedra
1,
Sergio Mella-Montecinos
2,5,6,
Andrés Opazo-Capurro
1,3,*,
Helia Bello-Toledo
1,3,
José M. Munita
3,7,
Juan C. Hormazábal
8 and
Gerardo González-Rocha
1,3,*
1
Laboratorio de Investigación en Agentes Antibacterianos, Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4070386, Chile
2
Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción 4070386, Chile
3
Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile
4
Centro Regional de Telemedicina y Telesalud del Biobío (CRT Biobío), Concepción 4030000, Chile
5
Unidad de Medicina Interna, Hospital Traumatológico de Concepción, Concepción 4030000, Chile
6
Unidad de Infectología, Hospital Regional “Dr. Guillermo Grant B.”, Concepción 4030000, Chile
7
Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina, Clínica Alemana Universidad del Desarrollo, Santiago 7550000, Chile
8
Subdepartamento de Enfermedades Infecciosas, Instituto de Salud Pública de Chile, Santiago 7780050, Chile
*
Authors to whom correspondence should be addressed.
Antibiotics 2022, 11(8), 1000; https://doi.org/10.3390/antibiotics11081000
Submission received: 21 June 2022 / Revised: 13 July 2022 / Accepted: 20 July 2022 / Published: 25 July 2022
(This article belongs to the Special Issue Epidemiology, Virulence Factors and Antimicrobial Resistance in Staphylococcus aureus)
Review Reports Versions Notes

Abstract

:
Macrolides, lincosamides, and type B streptogramins (MLSB) are important therapeutic options to treat methicillin-resistant Staphylococcus aureus (MRSA) infections; however, resistance to these antibiotics has been emerging. In Chile, data on the MLSB resistance phenotypes are scarce in both community-(CA) and hospital-acquired (HA) MRSA isolates. Antimicrobial susceptibility to MLSB was determined for sixty-eight non-repetitive isolates of each HA-(32) and CA-MRSA (36). Detection of SCCmec elements, ermA, ermB, ermC, and msrA genes was performed by PCR. The predominant clones were SCCmec I-ST5 (HA-MRSA) and type IVc-ST8 (CA-MRSA). Most of the HA-MRSA isolates (97%) showed resistance to clindamycin, erythromycin, azithromycin, and clarithromycin. Among CA-MRSA isolates, 28% were resistant to erythromycin, azithromycin, and 25% to clarithromycin. All isolates were susceptible to linezolid, vancomycin, daptomycin and trimethoprim/sulfamethoxazole, and over 97% to rifampicin. The ermA gene was amplified in 88% of HA-MRSA and 17% of CA-MRSA isolates (p < 0.001). The ermC gene was detected in 6% of HA-SARM and none of CA-SARM isolates, whereas the msrA gene was only amplified in 22% of CA-MRSA (p < 0.005). Our results demonstrate the prevalence of the cMLSB resistance phenotype in all HA-MRSA isolates in Chile, with the ermA being the predominant gene identified among these isolates.

1. Introduction

Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen involved in both human and animal infections [1,2]. Although MRSA was initially described as producing healthcare-associated infections (HA-MRSA), the appearance of community-associated MRSA infections (CA-MRSA) has been documented since the 1990s [3]. MRSA has shown a remarkable ability to develop resistance to a myriad of antibiotics, as well as to different disinfectants and heavy metals [4]. Vancomycin (VAN), a member of the glycopeptides, has been used as an important option to treat MRSA infections [5]. However, the risk of dissemination of vancomycin-resistant or non-fully susceptible strains suggests that this antibiotic should be used sparingly [6]. For this reason, macrolides (erythromycin [ERY]), lincosamides (clindamycin [CLI]), and streptogramins B (MLSB) have emerged as important therapeutic options to tackle CA-MRSA infections [7,8]. However, the increased use of these antimicrobials has favored the emergence of resistance to these drugs [9,10,11]. To date, there are three main MLSB resistance mechanisms described: i) changes in the ribosomal target site, which confers cross-resistance to the entire MLSB group [12]. This mechanism is conferred by ribosomal mutations or methylation of the 23S rRNA target site, which are mediated by the erm genes (mainly ermA, ermB, and ermC) [13,14]. Another mechanism corresponds to ii) an efflux-pump encoded by msrA, which can drive out 14- and 15-membered macrolides and streptogramin B, producing the MSB phenotype [15]. Finally, another mechanism iii) relies on drug inactivation and it only confers resistance to lincosamides due to an enzyme encoded by the lnu gene [11].
Significantly, the MLSB phenotype can be either constitutive (cMLSB) or inducible (iMLSB) [9]. Specifically, CLI, which is the MLSB agent used for the treatment of S. aureus infections, is a weak MLSB-resistance inducer and may lead to treatment failure due to false susceptibility results displayed in in vitro antimicrobial susceptibility tests [16]. Therefore, it is necessary to perform the CLI susceptibility test in the presence of a strong inducer, such as ERY [12]. Another key point is that antibiotic resistance genes that mediate the MLSB-resistance phenotype are found in mobile-genetic elements (MGEs) and, in consequence, may be horizontally transferred to susceptible strains [17]. In Latin America, the resistance rates to MLSB antibiotics have been reported to be 74% and 81% to ERY and CLI, respectively, among HA-MRSA isolates [10].
In Chile, S. aureus is one of the main etiological agents in health care-associated infections (HAIs) [18]. Specifically, it is the main cause of surgical wound infections (27%), and the second cause of pneumonia associated to invasive mechanical ventilation (21%). Likewise, it is involved in bloodstream infections (18%) and infections of the central nervous system (18%) [18]. Despite these data, the MLSB-resistance phenotype among HA- and CA-MRSA is still unknown among Chilean isolates. Therefore, the aim of our study was to detect and characterize the MLSB- and MSB-resistance phenotypes among HA-MRSA and CA-MRSA isolates collected between 2007 and 2017 from the S. aureus surveillance program of the National Institute of Public Health of Chile (ISP).

2. Results

2.1. Molecular Characterization of MRSA Isolates

All HA-MRSA (32) and CA-MRSA (36) isolates were resistant to FOX and mecA positive. For HA-MRSA, the Staphylococcal Cassette Chromosome mec (SSCmec) analysis revealed the presence of the Type I and Type II elements in 27 (84.4%) and 5 (15.6%) isolates, respectively. In addition, in all isolates classified as HA-MRSA, the absence of the pvl gene was confirmed. On the other hand, in all CA-MRSA (36), the pvl gene and the type IV SSCmec cassette were detected. Of these, 24 (66.7%) harbored the cassette subtype SSCmec IVc, whereas 11 (30.5%), and 1 (2.8%) amplified for the subtypes IVa and IVb, respectively; therefore, they were confirmed as CA-MRSA.
The MLST analyses of HA-MRSA showed that 27 (84.4%) isolates belonged to ST5 and 5 (15.6%) to ST105, whereas most CA-MRSA isolates belonged to the ST8 (27/36) (Table 1).

2.2. Antimicrobial Susceptibility Testing

The antibiotic resistance profiles were determined for both HA-MRSA and CA-MRSA isolates (Table 2). All isolates (32) of HA-MRSA were resistant to macrolides and to CLI. Moreover, 2 isolates (2/32) (6.3%) were also resistant to CHL and 1 isolate (1/32) (3.1%) to RIF. In the case of CA-MRSA, 9 isolates (9/36) (25%) were resistant to ERY, AZM and CLR, and one isolate was resistant to ERY and AZT (2.8%), but all were susceptible to CLI, CHL, and RIF (Table 2). All HA-MRSA, and CA-MRSA isolates were susceptible to LZD, VAN, DAP, and SXT (Table 3). Furthermore, the iMLS mechanism was detected in none of the two groups of MRSA isolates.
The HA-MRSA group showed more extended resistance profiles than CA-MRSA. Among the HA-MRSA, the most prevalent resistance profile was CLI-ERY-AZM-CLR, with 90.6% of isolates. On the other hand, in the CA-MRSA group, the most prevalent antibiotic resistance profile was ERY, AZM, and CLR, with 25% of isolates.

2.3. Prevalence of msrA and erm Genes

The ermA gene was amplified in 28 (87.5%) HA-MRSA isolates compared with 6 (16.7%) in CA-MRSA (p < 0.001). Additionally, the ermC gene was found in 2 (6.3%) of HA-MRSA and in none of CA-MRSA isolates (p > 0.05), and the ermB gene was detected in none of the isolates. On the other hand, msrA was detected in 11 (30.6%) of the CA-MRSA isolates, but in none of the HA-MRSA (p < 0.005) (Table 4).

3. Discussion

In recent years, we have observed an increased resistance to antibiotics, especially in those used for the treatment of serious infections associated with health care. MLSB group are antibiotics commonly used to treat skin and soft tissue infections caused by CA-MRSA [11]. The present study reports percentages of resistance to antibiotics in the MLSB group ≥90% in HA-MRSA. This finding agrees with the results of previous studies carried out with strains collected in Chile [10,19]. Besides, 20% of strains of CA-MRSA were resistant to MLSB group. These results show lower rates of resistance to these antibiotics in comparison to the official reports of the National Institute of Public Health of Chile (20% v/s 29%, respectively). On the other hand, our results showed higher values than previous reports that included strains isolated in Latin America, among both HA-MRSA (81% for ERY and 74% for CLI) and CA-MRSA [9,10,20,21,22,23,24].
Among the isolates included in this work, the predominant phenotype was the cMLSB phenotype. Molecular characterization of 68 MLSB-resistant MRSA revealed that among HA-MRSA, 87.5% were positive for ermA. However, in the CA-MRSA strains, 16.7% were positive for ermA, 6.3% for ermC, and 30.6% for msrA. The main mechanism of resistance to macrolides in CA-MRSA is mediated by the presence of the msrA gene, which results agree with previously published data [25].
Our results are in agreement with previous reports about the predominance of SCCmec type I-ST5 in HA-MRSA in Chile with classic resistance profiles of the Chilean/Cordobes clone that has a marked presence in hospitals of our country [10,26], and isolates of type IV-ST8 in CA-MRSA in Latin America, related to the USA-300 clone [10,19]. On the other hand, the dichotomy regarding the presence of MLSB or MSB resistance among HA-MRSA isolates highlights compared with CA-MRSA (97% vs approximately 25%, reaching statistical significance, p < 0.005). However, it is important to emphasize that these findings, which are consistent with the classic concept that hospital isolates of MRSA are multi-resistant and the community-based multi-susceptible and only resistant to β-lactams, should be monitored, since 20% of the isolates of CA-MRSA were resistant to antibiotics in this group, that is, 1 over 5 isolates were not widely susceptible. Accordingly, it is important to perform the proper laboratory detection of these phenotypes to analyze these isolates, since if the criterion of resistance to methicillin and broad susceptibility is the method of choice, other families, including those of the MLSB group, could obtain biased results.
All the strains analyzed are susceptible to VAN, LZD, DAP, and SXT, keeping these antibiotics as an alternative treatment within the therapeutic arsenal available in Chile, which is consistent with previous reports [10,18].
In summary, despite the higher frequency of the cMLSB phenotype than iMLSB in this study, we recommend performing the D test to identify clindamycin-induced resistance and guide therapeutic procedures in both HA-MRSA and CA-MRSA. Likewise, it is not recommended ruling out the submission of suspected CA-MRSA strains in surveillance programs based exclusively on the criterion of resistance only to β-lactams.

4. Materials and Methods

4.1. MRSA Isolates

Thirty-two non-repetitive HA-MRSA isolates recovered from eight Chilean cities between 2007 and 2017 (Table 5), and thirty-six CA-MRSA isolates collected in ten Chilean cities between 2012 and 2017 (Table 6) were included in this study. All isolates were selected from the biorepository maintained by the National Institute of Public Health of Chile (ISP), Santiago, Chile. All isolates were cryo-preserved at −80 °C in glycerol (50% v/v) and trypticase broth (2:1). The ISP criteria were used to define HA-MRSA and CA-MRSA [20].

4.2. Antimicrobial Susceptibility Testing

The cefoxitin test (FOX, 30 µg) for methicillin resistance detection, D-test, iMLSB, cMLSB, and MS phenotypes detection and antibiotics susceptibility determination, were performed by disk diffusion method on Mueller–Hinton agar following the CLSI recommendations and suggested breakpoints (2018) [27,28,29]. The antibiotics tested were erythromycin (ERY, 15 µg), clarithromycin (CLR, 15 µg), azithromycin (AZM, 15 µg), clindamycin (CLI, 2 µg), chloramphenicol (CHL, 30 µg), rifampicin (RIF, 5 µg), and trimethoprim/sulfamethoxazole (SXT, 25 µg).
The minimal inhibitory concentrations (MICs) of linezolid (LZD), vancomycin (VAN), and daptomycin (DAP) were determined using the broth microdilution method, according to CLSI guidelines and recommended breakpoints [28,29].

4.3. Characterization of MRSA Isolates

The presence of mecA, pvl in MRSA isolates, and the detection and characterization of the SCCmec element were performed by PCR-based protocols, as previously described [30,31,32]. Sequence types (ST) were obtained according to Opazo-Capurro et al. (2019), using the Pasteur’s scheme STs employing the bioinformatic tools available at the Center for Genomic Epidemiology (CGE) server (http://www.genomicepidemiology.org/, accessed on 13 March 2022) [33].

4.4. Molecular Detection of Antibiotic Resistance Genes

The detection of genes involved in the MLSB (ermA, ermB and ermC) and MSB (msrA) phenotypes were screened by conventional PCR according to protocols and primers previously described [34] (Supplementary Materials, Table S1).

4.5. Statistical Analyses

Pearson’s chi-squared test was used to determine associations between antibiotic resistance profiles, MLSB resistance genes, and MRSA types (CA or HA-MRSA). This was achieved utilizing the IBM SPSS Statistics version 23.0 software (SPSS Inc, Chicago, IL, USA), establishing statistical significance at p < 0.05 [35].

5. Conclusions

In Chile, in isolates of HA-MRSA, there is an evident predominance of ST5-SCCmec I, a Chilean/Cordobes clone, characteristically multiresistant, which includes resistance to antibiotics from the MLSB group; and susceptible to SXT and RIF. On the other hand, at the community level (CA-MRSA), there is an emergency of ST8-SCCmec IV, related to clone USA 300. Thus, microbiological surveillance of these isolates at the nosocomial level is required to verify whether the Chilean/Cordobes clone will be replaced by this community clone in Chile, and to monitor whether the latter will continue to increase its resistance to non-beta-lactam antibiotics, such as those of the MLSB group.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/antibiotics11081000/s1. Table S1: Primers used in this study.

Author Contributions

Conceptualization: M.Q.-A., A.A.-R., S.M.-M., A.O.-C., H.B.-T., G.G.-R.; methodology; software: M.Q.-A., A.A.-R., C.C., D.M., P.S.; validation: formal analysis: M.Q.-A.; data curation, M.Q.-A., A.A.-R., A.O.-C.; writing—original draft preparation: M.Q.-A.; writing—review and editing: M.Q.-A., A.A.-R., A.O.-C., S.M.-M., H.B.-T., J.M.M., J.C.H., G.G.-R.; visualization: M.Q.-A., A.O.-C., G.G.-R.; supervision: G.G.-R.; project administration: A.A.-R., G.G.-R. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by Universidad de Concepción, Grant VRID N° 218.085.040-1.0IN (A.A.-R.; G.G.-R.; H.B.-T.; S.M.-M.), the National Agency for Research and Development (ANID)/Scholarship Program/DOCTORADO NACIONAL/2017 21171278 (M.Q.-A.) and by FONDECYT 1171805, the National Fund for Scientific and Technological Development (FONDECYT) of Chile (J.M.M.).

Acknowledgments

We want to thank the microbiologists of the Chilean Hospitals and the National Institute of Public Health of Chile (ISP), who kindly provided the isolates for this study.

Conflicts of Interest

The authors declare that there are no conflict of interest.

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Table
Table 1. Sequence types (ST) of methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Table 1. Sequence types (ST) of methicillin-resistant Staphylococcus aureus strains isolated in Chile.
ST 5ST 8ST 30ST 105ST 868ST 923ST 2802Total
HA-MRSA2700500032
CA-MRSA1284011136
Table
Table 2. Antibiotic resistance profiles among methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Table 2. Antibiotic resistance profiles among methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Resistance ProfilesHA-MRSA *CA-MRSA *
CLIERYAZMCLRCHL2 (6.3)0
CLIERYAZMCLR 29 (90.6)0
CLIERYAZMCLRRIF1 (3.1)0
ERYAZMCLR 09 (25.0)
ERYAZM 01 (2.8)
All susceptible026 (72.2)
* No. of isolates (percentage), CLI: clindamycin, ERY: erythromycin, AZM: azithromycin, CLR: clarithromycin, CHL: chloramphenicol, RIF: rifampicin; HA-MRSA: Hospital-acquired methicillin-resistant Staphylococcus aureus; CA-MRSA: Community-acquired methicillin-resistant Staphylococcus aureus.
Table
Table 3. Minimum-inhibitory concentration (μg/mL) of some antimicrobials against methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Table 3. Minimum-inhibitory concentration (μg/mL) of some antimicrobials against methicillin-resistant Staphylococcus aureus strains isolated in Chile.
AntimicrobialsMIC50MIC90
Linezolid22
Vancomycin11
Daptomycin0.250.25
Table
Table 4. Antibiotic resistance, and presence of resistance genes in methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Table 4. Antibiotic resistance, and presence of resistance genes in methicillin-resistant Staphylococcus aureus strains isolated in Chile.
Percentage of Resistant Isolates to:Percentage of Resistance Genes:
CLIERYAZMCLRCHLRIFermAermBermCmsrA
HA-MRSA1001001001006.33.187.5000
CA-MRSA027.827.8250016.706.330.6
CLI: clindamycin, ERY: erythromycin, AZM: azithromycin, CLR: clarithromycin, CHL: chloramphenicol, RIF: rifampicin; HA-MRSA: Hospital-acquired methicillin-resistant Staphylococcus aureus; CA-MRSA: Community-acquired methicillin-resistant Staphylococcus aureus.
Table
Table 5. Hospital-acquired methicillin-resistant Staphylococcus aureus isolates from different Chilean cities.
Table 5. Hospital-acquired methicillin-resistant Staphylococcus aureus isolates from different Chilean cities.
CityNumber of Isolates
Santiago15
Rancagua2
Talca1
Concepción2
Los Ángeles1
Temuco3
Osorno1
Puerto Montt7
Total32
Table
Table 6. Community-acquired methicillin-resistant Staphylococcus aureus isolates from various Chilean cities.
Table 6. Community-acquired methicillin-resistant Staphylococcus aureus isolates from various Chilean cities.
CityNumber of Isolates
Valparaíso1
Viña del Mar1
Santiago14
Rancagua2
Talca1
Concepción5
Osorno1
Los Ángeles1
Temuco3
Puerto Montt7
Total36
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Quezada-Aguiluz, M.; Aguayo-Reyes, A.; Carrasco, C.; Mejías, D.; Saavedra, P.; Mella-Montecinos, S.; Opazo-Capurro, A.; Bello-Toledo, H.; Munita, J.M.; Hormazábal, J.C.; et al. Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile. Antibiotics 2022, 11, 1000. https://doi.org/10.3390/antibiotics11081000

AMA Style

Quezada-Aguiluz M, Aguayo-Reyes A, Carrasco C, Mejías D, Saavedra P, Mella-Montecinos S, Opazo-Capurro A, Bello-Toledo H, Munita JM, Hormazábal JC, et al. Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile. Antibiotics. 2022; 11(8):1000. https://doi.org/10.3390/antibiotics11081000

Chicago/Turabian Style

Quezada-Aguiluz, Mario, Alejandro Aguayo-Reyes, Cinthia Carrasco, Daniela Mejías, Pamela Saavedra, Sergio Mella-Montecinos, Andrés Opazo-Capurro, Helia Bello-Toledo, José M. Munita, Juan C. Hormazábal, and et al. 2022. "Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile" Antibiotics 11, no. 8: 1000. https://doi.org/10.3390/antibiotics11081000

APA Style

Quezada-Aguiluz, M., Aguayo-Reyes, A., Carrasco, C., Mejías, D., Saavedra, P., Mella-Montecinos, S., Opazo-Capurro, A., Bello-Toledo, H., Munita, J. M., Hormazábal, J. C., & González-Rocha, G. (2022). Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile. Antibiotics, 11(8), 1000. https://doi.org/10.3390/antibiotics11081000

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