Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Feces of the Small Indian Mongooses (Urva auropunctata) on Saint Kitts and Nevis, West Indies
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Sampling, Isolation and Identification
4.2. Sequencing and Sequence Analysis
4.3. Ethics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Butaye, P.; Argudín, M.A.; Smith, T.C. Livestock-Associated MRSA and Its Current Evolution. Curr. Clin. Microbiol. Rep. 2016, 3, 19–31. [Google Scholar] [CrossRef] [Green Version]
- Abdullahi, I.N.; Fernández-Fernández, R.; Juárez-Fernández, G.; Martínez-Álvarez, S.; Eguizábal, P.; Zarazaga, M.; Lozano, C.; Torres, C. Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern. Antibiotics 2021, 10, 1556. [Google Scholar] [CrossRef] [PubMed]
- Guardabassi, L.; Moodley, A.; Williams, A.; Stegger, M.; Damborg, P.P.; Halliday-Simmonds, I.; Butaye, P. High Prevalence of USA300 among Clinical Isolates of Methicillin-Resistant Staphylococcus aureus on St. Kitts and Nevis, West Indies. Front. Microbiol. 2019, 10, 1123. [Google Scholar] [CrossRef] [PubMed]
- Hoefer, A.; Boyen, F.; Beierschmitt, A.; Moodley, A.; Roberts, M.; Butaye, P. Methicillin-Resistant and Methicillin-Susceptible Staphylococcus from Vervet Monkeys (Chlorocebus sabaeus) in Saint Kitts. Antibiotics 2021, 10, 290. [Google Scholar] [CrossRef] [PubMed]
- Becker, A.A.M.J.; Hill, K.C.; Butaye, P. Unravelling the gut microbiome of the invasive small Indian mongoose (Urva au-ropunctata) in the Caribbean. Microorganisms 2021, 9, 465. [Google Scholar] [CrossRef] [PubMed]
- Bortolami, A.; Verin, R.; Chantrey, J.; Corrò, M.; Ashpole, I.; Lopez, J.; Timofte, D. Characterization of Livestock-Associated Methicillin-Resistant Staphylococcus aureus CC398 and mecC-positive CC130 from zoo animals in the United Kingdom. Microb Drug Resist. 2017, 23, 908–914. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Boyce, J.M.; Havill, N.L.; Maria, B. Frequency and Possible Infection Control Implications of Gastrointestinal Colonization with Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 2005, 43, 5992–5995. [Google Scholar] [CrossRef] [Green Version]
- Bhalla, A.; Aron, D.C.; Donskey, C.J. Staphylococcus aureus intestinal colonization is associated with increased frequency of S. aureuson skin of hospitalized patients. BMC Infect. Dis. 2007, 7, 105. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Monecke, S.; Gavier-Widén, D.; Hotzel, H.; Peters, M.; Guenther, S.; Lazaris, A.; Loncaric, I.; Müller, E.; Reissig, A.; Rup-pelt-Lorz, A.; et al. Diversity of Staphylococcus aureus isolates in European wildlife. PLoS ONE 2016, 11, e0168433. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hau, S.J.; Allué-Guardia, A.; Rusconi, B.; Haan, J.S.; Davies, P.R.; Frana, T.S.; Eppinger, M.; Nicholson, T.L. Single Nucleotide Polymorphism Analysis indicates genetic distinction and reduced diversity of swine-associated Methicillin Resistant Staph-ylococcus aureus (MRSA) ST5 isolates compared to clinical MRSA ST5 isolates. Front. Microbiol. 2018, 9, 2078. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Stefani, S.; Chung, D.R.; Lindsay, J.A.; Friedrich, A.W.; Kearns, A.M.; Westh, H.; Mackenzie, F.M. Meticillin-Resistant Staphy-lococcus aureus (MRSA): Global epidemiology and harmonisation of typing methods. Int. J. Antimicrob. Agents 2012, 39, 273–282. [Google Scholar] [CrossRef] [PubMed]
- Chen, C.-J.; Huang, Y.-C. New epidemiology of Staphylococcus aureus infection in Asia. Clin. Microbiol. Infect. 2014, 20, 605–623. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lowder, B.V.; Guinane, C.M.; Ben Zakour, N.L.; Weinert, L.A.; Conway-Morri, A.; Cartwright, R.A.; Simpson, A.J.; Rambaut, A.; Nübel, U.; Fitzgerald, J.R. Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus. Proc. Natl. Acad. Sci. USA 2009, 106, 19545–19550. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Strain * | MLST | Spa | SCCmec | Resistance Genes | Virulence Genes | Plasmid Replicon Types |
---|---|---|---|---|---|---|
M2-WF | 7434 | t0019 | blaZ | hlgA, hlgB, hlgC, seg, sei, sem sen, seo, seu tst, aur, splE | 16, 5a | |
M21-FB | 7434 | t0019 | blaZ | hlgA, hlgB, hlgC, seg, sei, sem sen, seo, seu tst, aur, splE | 16, 5a | |
M26a-PH | 5 | t002 | Iva(2B) | blaZ-mecA-mph©-msr(A)-aph(3’)-III-aac(6’)-aph(2’’) | sak, scn hlgA, hlgB, hlgC, lukD, lukE seg, sei, sej, sem sen, seo, sep, ser seu aur, splA, splB | 5a, US70, 13, 21 |
M27a-PH | 5 | t002 | IVa(2B) | blaZ-mecA-©(C)-msr(A)-aph(3’)-III-aac(6’)-aph(2’’) | sak, scn hlgA, hlgB, hlgC, lukD, lukE seg, sei, sej, sem sen, seo, sep, ser seu aur, splA, splB | 5a, US70, 13, 21 |
M30-PB | 5 | t002 | blaZ | sak, scn hlgA, hlgB, hlgC, lukD, luKE sec3, sed, seg, sei, sej, sel | 20 |
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Hoefer, A.; Becker, A.A.M.J.; Moodley, A.; Boyen, F.; Butaye, P. Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Feces of the Small Indian Mongooses (Urva auropunctata) on Saint Kitts and Nevis, West Indies. Antibiotics 2022, 11, 990. https://doi.org/10.3390/antibiotics11080990
Hoefer A, Becker AAMJ, Moodley A, Boyen F, Butaye P. Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Feces of the Small Indian Mongooses (Urva auropunctata) on Saint Kitts and Nevis, West Indies. Antibiotics. 2022; 11(8):990. https://doi.org/10.3390/antibiotics11080990
Chicago/Turabian StyleHoefer, Andreas, Anne A. M. J. Becker, Arshnee Moodley, Filip Boyen, and Patrick Butaye. 2022. "Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Feces of the Small Indian Mongooses (Urva auropunctata) on Saint Kitts and Nevis, West Indies" Antibiotics 11, no. 8: 990. https://doi.org/10.3390/antibiotics11080990
APA StyleHoefer, A., Becker, A. A. M. J., Moodley, A., Boyen, F., & Butaye, P. (2022). Presence of Methicillin Resistant Staphylococcus aureus (MRSA) in Feces of the Small Indian Mongooses (Urva auropunctata) on Saint Kitts and Nevis, West Indies. Antibiotics, 11(8), 990. https://doi.org/10.3390/antibiotics11080990