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Article

Klebsiella pneumonia in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence

by
Einas A. Osman
1,
Maho Yokoyama
2,
Hisham N. Altayb
3,
Daire Cantillon
4,
Julia Wille
5,6,
Harald Seifert
5,6,
Paul G. Higgins
5,6,7 and
Leena Al-Hassan
2,*
1
Bioscience Research Institute, Ibn Sina University, Khartoum 11111, Sudan
2
Department of Global Health and Infection, Brighton & Sussex Medical School, Brighton BN1 9PX, UK
3
Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia
4
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
5
Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50935 Cologne, Germany
6
German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, 50935 Cologne, Germany
7
Center for Molecular Medicine Cologne, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany
*
Author to whom correspondence should be addressed.
Antibiotics 2023, 12(2), 233; https://doi.org/10.3390/antibiotics12020233
Submission received: 26 December 2022 / Revised: 13 January 2023 / Accepted: 18 January 2023 / Published: 21 January 2023
(This article belongs to the Special Issue Molecular Characterization of Gram-Negative Bacteria: Antimicrobial Resistance, Virulence and Epidemiology)
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Abstract

:
The emergence and global expansion of hyper-virulent and multidrug resistant (MDR) Klebsiella pneumoniae is an increasing healthcare threat worldwide. The epidemiology of MDR K. pneumoniae is under-characterized in many parts of the world, particularly Africa. In this study, K. pneumoniae isolates from hospitals in Khartoum, Sudan, have been whole-genome sequenced to investigate their molecular epidemiology, virulence, and resistome profiles. Eighty-six K. pneumoniae were recovered from patients in five hospitals in Khartoum between 2016 and 2020. Antimicrobial susceptibility was performed by disk-diffusion and broth microdilution. All isolates underwent whole genome sequencing using Illumina MiSeq; cgMLST was determined using Ridom SeqSphere+, and 7-loci MLST virulence genes and resistomes were identified. MDR was observed at 80%, with 35 isolates (41%) confirmed carbapenem-resistant. Thirty-seven sequence types were identified, and 14 transmission clusters (TC). Five of these TCs involved more than one hospital. Ybt9 was the most common virulence gene detected, in addition to some isolates harbouring iuc and rmp1. There is a diverse population of K. pneumoniae in Khartoum hospitals, harbouring multiple resistance genes, including genes coding for ESBLs, carbapenemases, and aminoglycoside-modifying enzymes, across multiple ST’s. The majority of isolates were singletons and transmissions were rare.

1. Introduction

Klebsiella pneumoniae is an important global pathogen causing a variety of infections in community and healthcare-associated infections, such as pneumonia, urinary tract infections (UTI), and bloodstream infections. It poses a serious threat to human health and is one of the six highly virulent and antibiotic resistant bacterial pathogens: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE), requiring urgent global attention [1]. Multidrug resistance and carbapenem resistance in K. pneumoniae (MDR-Kp and CR-Kp) is of particular concern as treatment options are limited.
The population structure of K. pneumoniae appears to be diverse yet highly structured, with distinct clonal groups (CG) subdivided into MDR- and hypervirulent (Hv)-clones [2]. A subset of these clones contribute to global diseases and outbreaks and are referred to as ‘global problem clones’, involving the transfer and spread of antibiotic resistance genes and endemic plasmids in highly disseminating successful clones worldwide [3].
Antimicrobial resistance (AMR) is a global health issue, with low- and middle-income countries (LMICs) carrying the largest burden of infection and AMR, both in the community and healthcare settings [4,5]. Global efforts such as the World Health Assembly’s Global Action Plan on AMR (GAP-AMR), which was followed by the adoption of National Action Plans (NAP)s by member countries, and the World Health Organisation (WHO) Global Antimicrobial Resistance and Use Surveillance System (GLASS) are all strategies to contain AMR [6]. However, lack of systematic surveillance and scarcity of data on the burden of infectious diseases and AMR is largely due to limited infrastructure and financial support.
Sudan is a lower middle-income country in Africa, with a population of approximately 44 million (in 2021) covering an area of 1.8 million km2. Median age is 19.7, and life expectancy at birth is 64.1 years [7]. Sudan’s health care system is fragile, hospital-centric, and fragmented in both the public and private sectors, and facing several challenges. The WHO data show that health-associated out-of-pocket expenditure paid by households are ~74%, which cover curative care, medicines, and medical consumables [7]. There is disparity in the distribution of health care personnel between the public and private sectors and between urban and rural areas. Moreover, the high turnover and migration of health professionals continue to threaten the capacity to respond to the increased demand for health services [7]. An analysis of the AMR situation by the Sudanese NAP revealed high rates of resistance, and an urgent need to address the situation by improving awareness, surveillance, hygiene, and infection control, and the optimization of antimicrobial medicines (https://www.who.int/publications/m/item/sudan-national-action-plan-on-antimicrobial-resistance; last accessed on 7 January 2023). However, the implementation of antimicrobial stewardship and improving the access to antimicrobials is difficult due to the lack of supervisory systems to ensure rational prescribing, combined with poor accessibility of essential antibiotics in rural areas.
MDR K. pneumoniae is on the WHO global priority pathogen list [8], but despite global efforts there are significant gaps in robust epidemiological data, particularly in LMICs. Recognising the significance of MDR- and CR-Kp globally and the need for accurate epidemiological typing of the organism, it is important to gain insight into circulating clones in order to contextualise data on national and international lineages, and to inform locally-relevant infection prevention and control based on local data. In Sudan, K. pneumoniae is highly prevalent in hospitals and has been reported in numerous studies as the most common Gram-negative organism identified [9,10,11]. K. pneumoniae was the second most frequent organism contributing to urinary tract infection (UTI) in diabetic patients [12]. In a study on the presence of pathogenic bacteria isolated from bank notes in Khartoum, K. pneumoniae was the most prevalent organism identified [13]. Despite this prevalence, few studies have included molecular methods for isolate identification, confirmation, or resistance surveillance. We have previously conducted a study on the local epidemiology of CR-Kp in Khartoum by using MLST [14]. In this study we used whole genome sequencing (WGS) to generate more robust data on the molecular epidemiology, transmission, resistome, and virulence profiles of MDR K. pneumoniae from multiple hospitals in Khartoum, Sudan.

2. Results

A total of 86 isolates were included in this study from five different hospitals in the Sudanese capital of Khartoum: 2 isolates from 2016, 24 from 2018–2019, and 60 from March–September 2020 (detailed sampling procedure in the Methods). Eighty-four K. pneumoniae isolates were confirmed by MALDI-TOF and Kleborate, while 2 isolates (LH_F129 and LH_F72) were identified as Klebsiella quasipneumoniae subsp. quasipneumoniae and Klebsiella variicola subsp. variicola, respectively. The organisms came from a variety of samples: blood (n = 25, 29.4%), pus (n = 2, 2.4%), sputum (n = 9, 10.6%), urine (n = 37, 43.5%), and wound swabs (n = 12, 14.1%) (Figure 1; Table 1).
Most isolates came from Fedail Hospital, followed by Ribat University Hospital, both of which are two of the largest hospitals in Khartoum. Urine and blood samples were most common across all isolates.
Sixty-eight isolates (80%) were MDR (resistant to >3 antibiotic classes), 35 of which (51%) were carbapenem resistant (imipenem andor meropenem MIC ≥ 4 mgL). The remaining 16 isolates were susceptible to all tested antibiotics. All isolates were colistin susceptible (MIC < 2 mgL). Multiple resistance mechanisms were identified (Table 2 and Supplementary Table S1), contributing to the observed MDR phenotypes. Aminoglycoside resistance was present in 49 isolates (57%), mediated by multiple aminoglycoside-modifying enzymes. β-lactam resistance was detected in 58 isolates and was mediated by acquired β-lactamases: OXA-1 (n = 19), OXA-9 (n = 4, two of which were co-harbouring OXA-1 and TEM-1), CMY (n = 3, one of which was co-harbouring OXA-1), DHA-1 (n = 3, two of which were co-harbouring TEM-1), and SCO-1 (n = 3, one of which was co-harbouring TEM-1); TEM-1 was the most prevalent β-lactamase, identified in 40 isolates. The acquired extended-spectrum β-lactamase (ESBL) CTX-M-15 was present in 64 isolates, and CTX-M-14 was present in four isolates, three of which co-harboured CTX-M-15. Seventeen isolates did not harbour any ESBLs. SHV-variants were present in all K. pneumoniae isolates.
Carbapenem resistance was mediated by NDM-1 (n = 23), NDM-4 (n = 1), and NDM-5 (n = 11, three of which co-harboured OXA-48); one isolate harboured OXA-48 on its own, and one isolate harboured OXA-232 (OXA-48-like enzyme). Three isolates displayed phenotypic carbapenem resistance, but no acquired carbapenemases were detected, and the observed carbapenem resistance was associated with a combination of an ESBL and modifications in the OmpK35OmpK36 (Table 2 and Table S1). LH_F164 possessed a truncated blaNDM-1 gene but also harboured OXA-1, CTX-M-15, and a nucleotide substitution (G690A) in the OmpK35 encoding gene, leading to a premature stop codon. LH_R313, on the other hand, lacked an ompK35 gene, and carbapenem resistance was mediated by a modification of OmpK36 containing an LSP insertion in the amino acid sequence at position 184.
A total of 37 different sequence types (STs) were identified in the study, highlighting the epidemiological diversity of the isolates. We observed 14 small transmission clusters (TC) by cgMLST, with isolates differing by 0–5 alleles (Figure 2). TC-1 (ST17) comprises three isolates collected from Al-Saha Hospital (ASH) in 2019; however, they differed in their respective resistomes. Metadata relating to the location of patients or date of isolation are not available for these isolates. LH_HishK11 and LH_HishK13 are both carbapenem- susceptible, while LH_HishK12 is MDR and harbours multiple aminoglycoside-modifying enzymes (aac(3)-IIa-like;aac(6’)-Ib-cr-like;aadA5) and β-lactamases: blaCTX-M-15, blaOXA-1, and blaNDM-1. All other TCs comprise isolates with identical resistomes (Figure 2 and Table S1).
The most frequently isolated ST in the study was ST147 (n = 9), followed by ST20 (n = 7), ST437 (n = 7), ST101 (n = 6), ST15 (n = 6), ST307 (n = 6), ST383 (n = 5), and ST17 (n = 4), all of which are high-risk global clones (GC), defined as epidemic high-risk clones over-represented globally [2], with the remaining 29 singleton STs. Isolates within the same ST exhibited some similarities at the genomic level, but as seen in Table 2 and Table S1, some had different resistance phenotypes and genes. For example, ST147 isolates came from multiple hospitals, with isolates from FH all collected within a 1-month timeframe (July–August 2020), but they differed by ≥21 alleles and transmission was ruled out. Moreover, differences were seen in their virulence and resistome profiles, with LH_F134 harbouring no recognised virulence factors, while LH_F149, LH_F18, and LH_F97 all harboured ybt9 on a ICEKp3 structure. LH_F134, LH_F149, and LH_F18 were carbapenem resistant, mediated by blaNDM-1; however, LH_F97 was carbapenem susceptible. Similar data are observed in ST-147 isolates from RUH which had identical resistomes, except R344, which harboured blaNDM-5 and not blaNDM-1 and differed by >50 allelic differences per the cgMLST and was therefore not part of a single transmission cluster. The only TC in this group were two identical isolates from Al Baraha Hospital, which also had identical resistomes.
ST20 was also found in multiple hospitals (SUH, ASH, ABH, and RUH), and comprised one TC from two hospitals (TC-2; Figure S1), but there were differences in their resistome. All ST437 were MDR and contained the hypervirulence gene ybt9 on an ICEKp3 structure. This ST comprised four identical isolates forming a TC (TC-2) with two other isolates, and a singleton. Key resistome differences were observed in aminoglycoside-modifying enzymes and carbapenemases: LH_K4 (collected in 2018 from ABH) had aac(3)-IId-like;aac(6’)-Ib’.v1;aadA;aph(3’)-VI and blaNDM-1, whereas the remaining isolates (collected from FH in 2020) all had rmtB and blaNDM-5. The imipenem MIC was also notably less for LH_K4, harbouring blaNDM-1 at 64 mgL vs. >128 mgL for the remaining NDM-5 isolates.
ST101 was only present in isolates in 20182019 (SUH, BH; Supplementary Figure S1) and comprised five identical isolates (TC-11) and a singleton. All ST-101 isolates were MDR, but only two were carbapenem-resistant: LH_HishK5 and LH_HishK7, mediated by NDM-1 (LH_HishK5) and modification in ompK35 (G947A, leading to a premature stop codon at position 316), combined with blaOXA-1 and blaCTX-M-15 (LH_HishK7).
All ST15 isolates were MDR (and comprised two, TC-12 and TC-13, and two singletons), but were carbapenem-susceptible, except for LH_F164, which was carbapenem-resistant (imipenem MIC 16 mgL), associated with a G690A substitution in the ompK35 gene (leading to a premature stop codon) combined with blaOXA-1. LH_F164 was the only strain which was also positive for ybt9 (on ICEK3p) within this ST. Isolates within ST307 came from two different hospitals (RUH and FH), with 56 isolates from RUH. Three of these isolates form a transmission cluster. Interestingly, one isolate, collected 4 months later in July 2020 (LH_R275) from RUH appears to have lost the MDR and CR pheno- and geno-types.
The ST-383 isolates were all MDR, CR, and were comprised of three singletons from Fedail Hospital, and one TC from Soba University Hospital (TC-8). Of these, 35 (LH_HishK8, LH_HishK9, and LH_F190) isolates co-harboured multiple resistance genes: blaCTX-M-14 and blaCTX-M-15, and blaNDM-5 and blaOXA-48. The remaining two isolates (LH-F15 and LH-F35) harboured either blaCTX-M-14 and blaOXA-48 or blaCTX-M-15 and blaNDM-5, respectively. Of the three ST17 isolates, only one was MDRCR and harboured blaNDM-1.
Of the remaining 29 singleton STs, 11 of them were MDR and CR, whereas the remaining 18 were susceptible to all tested antibiotics.
Thirty-six isolates harboured one or more virulence genes, namely ybt9, ybt10, ybt4, ybt14, iuc, rmp1, KpVP-1, and rmpA2 (Table 2 and Table S2). Yersiniabactin ybt9 located on ICEKp3 was the most common structure detected (n = 21), found in ST147, ST101, ST15, and ST437 (all of which are GCs), as well as ST11, and was present in multiple isolates and hospitals in our collection. Seven isolates harboured ybt10 on ICEKp4, associated with ST530, ST307 (GC), ST3161, and ST45. Aerobactin iuc1 was associated with ST383 (n = 4, of which two co-harboured rmp1), and 1 ST231 co-harboured ybt14 and iuc.
Forty different K-loci were identified, as detailed in Supplementary Table S2, along with seven variants of O-loci, most common of which were O1 (n = 30) and O2 (n = 21). The O-locus variant could not be identified in 10 isolates.

3. Discussion

The aim of this study was to conduct a snapshot analysis of the molecular epidemiology of the K. pneumoniae population from hospitals in Khartoum, Sudan. We have included isolates from five hospitals through several sampling timeframes over the years. K. pneumoniae was implicated in multiple infections, including blood, skin and soft tissue (SST), and urine. The majority of isolates (81%) were MDR, of which more than half were also carbapenem resistant, mediated mainly by NDM-1-, NDM-5-, and OXA-48-like enzymes. Interestingly, KPC was absent from the isolate collection, despite its global prevalence in CR-Kp, particularly in ST258. Carbapenem resistance was also mediated by the presence of an ESBL and modifications in OmpK35 and OmpK36 in three isolates (Table 2). Modifications in outer-membrane porins which restricts antibiotic entry is an important carbapenem resistance mechanism [15]. OmpK35 is usually truncated due to a mutation encoding a frame shift that results in a premature stop codon [16,17]. OmpK36 is more heterogenous, rarely truncated, and resistance mutations are common leading either to abundance of OmpK36 in the outer membrane or constriction of the pore size [15,18]. Carbapenem MIC for LH_R290 showed intermediate resistance (IMI 4 mgL) without an acquired carbapenamase; however, the isolate harbours SCO-1, a plasmid-encoded ESBL able to hydrolyse not only penicillins but also, to a lesser degree, cephalosporins and carbapenems. Since its discovery in 2007, the blaSCO-1 gene (GenBank accession no. EF063111) has been identified in Acinetobacter baumannii, Escherichia coli, Serratia marcescens, Klebsiella aerogenes, Salmonella enterica, and only four K. pneumoniae isolates [19].
The epidemiological analysis of K. pneumoniae in Sudan revealed a large diversity of 37 different STs, with 13 transmission clusters (Figure 1). Five of these clusters have been collected from different hospitals, thereby indicating intra-hospital circulation of clones. Global problem high-risk MDR clones (GC), identified by Wyres et al., 2020, have been found in this study; however, we noted that not all were MDR. We have identified multiple losses of resistance within these GCs over time. For example, isolates in ST307 at RUH collected in FebruaryMarch 2020 were carbapenem-resistant; however, isolate LH_R275 collected in July 2020 was neither MDR nor CR. Similarly, isolates within ST147: LH_F134, LH_F149, LH_F18, were CR harbouring blaNDM-1; however, LH_F97 (isolated from the same hospital) is carbapenem-susceptible, but had acquired the ybt9 virulence gene on ICEKp3. ST437 isolates appear to have lost blaNDM-1 (from 2018) and acquired blaNDM-5 in the isolates collected in 2020. However, we did not specifically look for plasmids in this study, and further detailed investigations of possible plasmid acquisition and loss events must be conducted to confirm the observed results. The differences in the resistomevirulomes of isolates within the same ST and transmission cluster is important to note for outbreak and epidemiological studies. The data provided by genomic analysis are an important tool where molecular epidemiology is combined with patient clinical data and the resistomes to obtain accurate information on transmission of resistant and virulent pathogens.
Yersinibectin (ybt9 and ybt10) were the most identified virulence genes in the study and were mostly associated with GCs: ST101, ST147, and ST307, in addition to their occurrence in singleton non-GCs, such as ST39, ST437, and ST882 (Table 2). ybt is usually present in 30–40% of K. pneumoniae human HAI isolates and up to 13% of community-acquired isolates [20]. Other virulence genes also identified in the study included aerobactin, iuc1, and hypermucoidy-associated rmpA genes. We have noted AMR-hypervirulence convergence events in 24 isolates, which harboured a virulence gene and were MDR: ST530, ST11, ST147, ST101, ST231, ST307, ST15, ST383, ST437, and ST45. It is important to note that we have not confirmed the virulence phenotypes of the isolates, and more research into the clinical significance of hypervirulence is required.
ST383 isolates were particularly unique in their resistomes and virulomes. The isolates co-harboured multiple ESBLs and carbapenemases simultaneously, CTX-M-14 and CTX-M-15, in addition to NDM-5 and OXA-48 in the same isolates and iuc-1, rmp1 on KpVP-1, and rmpA2. ST383 is a prevalent MDR clone in China, Australia, the UK, and Germany [21]. In a study from Egypt, ST383 was identified in a single isolate simultaneously encoding CTX-M-14 (on an IncLM plasmid) and CTX-M-15 (encoded on a hybrid IncHI1BIncFIB plasmid) in addition to both NDM-5 and OXA-48 [22]. Results by PlasmidFinder show the same plasmids are present in our ST383 isolates (incFIBIncHI1B and incL).This clearly indicates the endemicity of this particular clone in the region, as the data presented in the Egyptian isolate is identical to that in our ST383 cluster.
In our previous study on K. pneumonia isolated in Khartoum, 2015–2016, we performed MLST on 117 isolates. All were MDR, and 42.8% were CR-Kp [14]. A similar diversity was observed in the current study, with 52 different STs, with the most common being ST383 (n = 8), ST101 (n = 5), and ST48 (n = 2), in addition to assignment of 15 novel STs. NDM was the most common carbapenemase, in addition to VIM, which has not been detected in the current study. A study conducted in Sudan by Adam et al. from 2015–2016 [23] revealed that metallo-β-lactamases (VIM and IMP) were prevalent in K. pneumoniae (1520 isolates), and found in combination with NDM in 520 isolates, in contrast to our study where VIM and IMP were not detected and NDM was the most prevalent MBL. Comparing the data from these studies indicates the maintenance of ST101, ST383, ST219, and ST437 lineages in Khartoum hospitals over the years, and the presence of novel lineages, as reported in this study.
In comparison to the present study, we also investigated A. baumannii from several different hospitals in Khartoum during the same time period and found that the A. baumannii isolates were mostly transmissions and very few were singletons [24]. This highlights that even in an area that obviously has a high incidence of hospital-acquired infections and patient-to-patient transmissions, including between hospitals, K. pneumoniae is not easily transmitted. While most K. pneumoniae in this study were not transmissions, they do harbour similar carbapenemases, which may indicate transmission of resistance genesmobile genetic elements, which will be the focus of a follow-up study.
When compared to other African studies, the K. pneumoniae population in Southern Nigeria was found to contain four dominant lineages: ST307, ST524, ST15, and ST25, while CR remained low at 8%, with no isolate carrying a combination of carbapenemases, in contrast to our study, where carbapenem-resistance was much higher (41.6% of 84 isolates) and three strains co-harboured NDM and OXA-48 [25]. A multi-centre pilot study in Egypt revealed that ST11, ST147, ST231, ST383, and ST101 were prevalent, which is similar to our study [26]. The observed similarities and difference with the local, regional, and international studies highlights the diversity and dynamic nature of the representative local population of K. pneumoniae as well as the potential for regional dissemination of clones in different countries, and the need to support local capacity in robust epidemiological surveillance. The first WGS study on a single ST14 K. pneumoniae from Sudan was conducted in 2019 [27]. To the best of our knowledge, this is the first WGS-based study of a large collection of K. pneumoniae from Sudan.
Through this study, we aimed to generate robust epidemiological data on K. pneumoniae; albeit acknowledging some limitations. We have tried to collect as many K. pneumoniae isolates within the indicated timeframes. However, we cannot exclude sampling bias (such as missed samples or misidentification), as the study team was not directly involved in the sampling procedures. Routine sampling of infected patients is not always performed in local hospitals in Sudan, and sampling is frequently sporadic and based on individual doctors’ rather than hospital policiesguidelines due to poor microbiological infrastructure in hospitals. The fragmented health service, and the large out-of-pocket contribution by individuals and households, as presented in the introduction, lead to some hospitals also outsourcing microbiology to private laboratories. This study, however, focused on data from hospital-based laboratories only. Furthermore, the study was interrupted during COVID-19 lockdowns, so isolates between April and July 2020 were not collected. Some associated demographic data were also lacking for some isolates collected in 2018–2019. The sampling strategy therefore cannot exclude missed transmissions. Nevertheless, this study provides a starting point for the understanding of the population structure and diversity of K. pneumoniae in Khartoum, Sudan. It furthermore supports national and global efforts in providing robust epidemiological information on important HAI. This study highlights the importance of using WGS in AMR surveillance. Despite the vast possibilities for implementation, major challenges relating to capacity and training, and particularly analysis, of WGS still exist in many LMICs [28].

4. Materials and Methods

A total of 86 non-repetitive isolates were collected within these timeframes: Twenty-four isolates in 2018–2019 from Al-Baraha Hospital (ABH), Al-Saha Hospital (ASH), and Soba University Hospital (SUH); then 60 isolates in March–September 2020 from Fedail Hospital (FH), Ribat University Hospital (RUH), and SUH. Two additional isolates (from 2016) were included from RUH. Descriptive statistics was used to summarise the details of each isolate, including date of isolation, sample type, and hospital (Figure 1 and Table 1). The clinical microbiology laboratories in the named hospitals identify clinical specimens to the genus levels by conventional phenotypic and biochemical methods (growth and colony morphology on various media, and biochemically: indole negative, MR negative, VP positive, citrate positive, oxidase negative, and catalase positive). No specific selection criteria were implemented, as we aimed to collect and characterise any K. pneumoniae isolates identified in the hospitals during the study periods. Upon identification of K. pneumoniae by the clinical microbiology laboratories, the isolates were stored at 4 °C and collected by the study team within 48 h. It is important to note that the study team cannot guarantee any misidentification or loss of samples that were not submitted to the study.
All acquired isolates were subsequently confirmed phenotypically by conventional culture methods to exclude any contamination, then genotypically by amplification of 16S-23S rDNA internal transcribed spacer (ITS) of K. pneumoniae, as described in our preceding study [29]. Further identification confirmation was performed by MALDI-TOF prior to WGS.
Antimicrobial susceptibility (AST) was initially performed by disk-diffusion at the clinical microbiology laboratories and interpreted according to CLSI guidelines [30]. Upon confirmation of species, as described above, the minimum inhibitory concentration (MIC) was determined by MICRONAUT-GN, (Merlin Diagnostika, Germany). This system allows the determination of the MIC for a panel of Gram-negative specific antibiotics: meropenem (MER), gentamicin (GEN), amikacin (AMK), trimethoprim-sulfamethoxazole (SXT), ciprofloxacin (CIP), colistin (COL), Amoxicillin (AMX), AmoxicillunClavulanate (AMC), Cefotaxime (CTX), Ceftazidime (CAZ), Cefuroxim (CXM), Etrapenem (ERT), and Temocillin (TMO), with two concentrations (mgL) based on the EUCAST MIC breakpoints for sensitivity (S) or resistance (R) in a single microtiter plate. Additionally, MIC for imipenem (IMI) was performed by broth microdilution according to the EUCAST guidelines V2, 2020 [31]. Quality control strains E. coli ATCC 13846, ATCC 12241, and P. aeruginosa ATCC12903 were used in all AST experiments. Multidrug resistance was defined as resistance to 3 or more antimicrobial classes.
Total DNA was extracted from the bacterial isolates using a MagAttract HMW DNA Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. Sequencing libraries were prepared using the Nextera XT library prep kit (Illumina GmbH, Munich, Germany) for a 250-bp paired-end sequencing run on an Illumina MiSeq platform. De novo assembly was constructed using Velvet v1.1.04. Molecular epidemiology of the isolates was investigated by core-genome MLST (cgMLST) using Ridom® SeqSphere+ version 8.5.1 [32].
Kleborate v2.0.4 was used to screen genome assemblies for Sequence Types (STs), capsular type, and virulence loci [33]. The resistome for the assembled genomes was identified using Kleborate v2.0.4 and the Resfinder database v3.2 https://cge.cbs.dtu.dkservicesResFinder (accessed on 10 February 2021) [34]. Sequence alignment and visualisation was performed by Geneious Prime.
All assembled genome sequences were submitted to Genbank under BioPproject ID PRJNA912084.

5. Conclusions

This study aims to provide a population snapshot of K. pneumoniae in Sudan. ST101, ST147, and ST437 are epidemic and predominantly present in multiple hospitals. MDR and CR-Kp were also prevalent (80% and 50%, respectively), which is alarming. The detection of multiple virulence genes and the potential emergence of AMR-hypervirulence convergence events must also be considered for local surveillance. We conclude that a diverse population of K. pneumoniae is present in hospitals in Khartoum, Sudan. Further genomic investigations, and inclusion of more data from national and regional hospitals, would enable delivery of value on a local, national, regional, and global level in understanding the pathogen dynamics.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/antibiotics12020233/s1. Figure S1: Minimum spanning tree (MST) of 84 K. pneumoniae by hospital information. Ridom SeqSphere+ MST for 84 samples based on 2358 columns, pairwise ignoring missing values, logarithmic scale. Cluster distance threshold: 15. Isolates grouped by colour indicate the different hospitals. Samples were collected from five different hospitals, and 37 different STs were identified, in addition to 14 transmission clusters, represented by shaded nodes and arrows. Numbers between the nodes indicate the number of allelic differences; Table S1: Sequence types, antimicrobial susceptibility and associated resistome; Table S2: Details of ST with virulence genes, K and O loci.

Author Contributions

Conceptualization, E.A.O., H.N.A. and L.A.-H.; methodology, E.A.O., H.N.A. and L.A.-H.; validation, P.G.H., J.W. and L.A.-H.; formal analysis, M.Y. and L.A.-H.; investigation, E.A.O., D.C., J.W. and L.A.-H.; resources, E.A.O., L.A.-H., D.C. and P.G.H.; data curation, M.Y.; writing—original draft preparation, E.A.O. and L.A.-H.; writing—review and editing, E.A.O., M.Y., H.N.A., D.C., J.W., H.S., P.G.H. and L.A.-H.; visualization, M.Y. and P.G.H.; supervision, L.A.-H. and H.N.A.; project administration, E.A.O. and L.A.-H.; funding acquisition, L.A.-H. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the University of Sussex: International Development Challenges Fund, grant number IDCF1-007.

Institutional Review Board Statement

Formal permission was obtained from all hospitals included in this study, and from the Research Ethics Committee, Ministry of Health, Sudan.

Informed Consent Statement

Patient consent was waived by the Sudanese Research Ethics Committee. All patients consent that any microbiological samples collected in the hospital as part of routine clinical work may be used for research purposes. No patient identifying details were collected for this study.

Data Availability Statement

The genome assemblies presented in this study are openly available in NCBI GenBank under BioProject ID PRJNA912084.

Acknowledgments

We would like to acknowledge the medical and technical staff of the microbiology laboratories in the participating hospitals for their help in isolate and data collection. We also want to acknowledge Alaa M. Albasha, Maram M. Elnosh, and Esraa H. Osman for their contribution to part of the isolate collection.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Boucher, H.W.; Talbot, G.H.; Bradley, J.S.; Edwards, J.E.; Gilbert, D.; Rice, L.B.; Scheld, M.; Spellberg, B.; Bartlett, J. Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America. Clin. Infect. Dis. 2009, 48, 1–12. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Wyres, K.L.; Lam, M.M.C.; Holt, K.E. Population Genomics of Klebsiella Pneumoniae. Nat. Rev. Microbiol. 2020, 18, 344–359. [Google Scholar] [CrossRef] [PubMed]
  3. Navon-Venezia, S.; Kondratyeva, K.; Carattoli, A. Klebsiella Pneumoniae: A Major Worldwide Source and Shuttle for Antibiotic Resistance. FEMS Microbiol. Rev. 2017, 41, 252–275. [Google Scholar] [CrossRef] [PubMed]
  4. Silvester, R.; Madhavan, A.; Kokkat, A.; Parolla, A.; Adarsh, B.M.; Harikrishnan, M.; Abdulla, M.H. Global Surveillance of Antimicrobial Resistance and Hypervirulence in Klebsiella Pneumoniae from LMICs: An in-Silico Approach. Sci. Total Environ. 2022, 802, 149859. [Google Scholar] [CrossRef] [PubMed]
  5. Murray, C.J.; Ikuta, K.S.; Sharara, F.; Swetschinski, L.; Robles Aguilar, G.; Gray, A.; Han, C.; Bisignano, C.; Rao, P.; Wool, E.; et al. Global Burden of Bacterial Antimicrobial Resistance in 2019: A Systematic Analysis. Lancet 2022, 399, 629–655. [Google Scholar] [CrossRef] [PubMed]
  6. WHO. Geneva Global Action Plan on Antimicrobial Resistance; World Health Organization: Geneva, Switzerland, 2017; pp. 1–28. [Google Scholar]
  7. WHO. Country Cooperation Strategy for WHO and Sudan; World Health Organization: Geneva, Switzerland, 2022; ISBN 9789290229698. [Google Scholar]
  8. WHO. Global Priority List of Antibiotic-Resistant Batceria to Guide Research, Discovery, and Development of New Antibiotics; WHO: Geneva, Switzerland, 2017; p. 7. [Google Scholar]
  9. Alsammani, M.A.; Ahmed, M.I.; Abdelatif, N.F. Bacterial Uropathogens Isolates and Antibiograms in Children under 5 Years of Age. Med. Arch. 2014, 68, 239–243. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  10. Albasha, A.M.; Osman, E.H.; Abd-Alhalim, S.; Alshaib, E.F.; Al-Hassan, L.; Altayb, H.N. Detection of Several Carbapenems Resistant and Virulence Genes in Classical and Hyper-Virulent Strains of Klebsiella Pneumoniae Isolated from Hospitalized Neonates and Adults in Khartoum. BMC Res. Notes 2020, 13, 312. [Google Scholar] [CrossRef]
  11. Elbadawi, H.S.; Elhag, K.M.; Mahgoub, E.; Altayb, H.N.; Abdel Hamid, M.M. Antimicrobial Resistance Surveillance among Gram-Negative Bacterial Isolates from Patients in Hospitals in Khartoum State, Sudan [Version 1; Peer Review: Awaiting Peer Review]. F1000Research 2019, 8, 156. [Google Scholar] [CrossRef] [Green Version]
  12. Hamdan, H.Z.; Kubbara, E.; Adam, A.M.; Hassan, O.S.; Suliman, S.O.; Adam, I. Urinary Tract Infections and Antimicrobial Sensitivity among Diabetic Patients at Khartoum, Sudan. Ann. Clin. Microbiol. Antimicrob. 2015, 14, 26. [Google Scholar] [CrossRef] [Green Version]
  13. Abd Alfadil, N.A.; Suliman Mohamed, M.; Ali, M.M.; El Nima, E.A.I. Characterization of Pathogenic Bacteria Isolated from Sudanese Banknotes and Determination of Their Resistance Profile. Int. J. Microbiol. 2018, 2018, 4375164. [Google Scholar] [CrossRef]
  14. Osman, E.A.; El-Amin, N.E.; Al-Hassan, L.L.; Mukhtar, M. Multiclonal Spread of Klebsiella Pneumoniae across Hospitals in Khartoum, Sudan. J. Glob. Antimicrob. Resist. 2021, 24, 241–245. [Google Scholar] [CrossRef] [PubMed]
  15. David, S.; Wong, J.L.C.; Sanchez-Garrido, J.; Kwong, H.S.; Low, W.W.; Morecchiato, F.; Giani, T.; Rossolini, G.M.; Brett, S.J.; Clements, A.; et al. Widespread Emergence of OmpK36 Loop 3 Insertions among Multidrug-Resistant Clones of Klebsiella Pneumoniae. PLoS Pathog. 2022, 18, e1010334. [Google Scholar] [CrossRef] [PubMed]
  16. Wassef, M.; Abdelhaleim, M.; AbdulRahman, E.; Ghaith, D. The Role of OmpK35, OmpK36 Porins, and Production of β-Lactamases on Imipenem Susceptibility in Klebsiella Pneumoniae Clinical Isolates, Cairo, Egypt. Microb. Drug Resist. 2015, 21, 577–580. [Google Scholar] [CrossRef]
  17. Rodrigues, C.; Desai, S.; Passet, V.; Gajjar, D.; Brisse, S. Genomic Evolution of the Globally Disseminated Multidrug-Resistant Klebsiella Pneumoniae Clonal Group 147. Microb. Genomics 2022, 8, 000737. [Google Scholar] [CrossRef]
  18. Wong, J.L.C.; Romano, M.; Kerry, L.E.; Kwong, H.S.; Low, W.W.; Brett, S.J.; Clements, A.; Beis, K.; Frankel, G. OmpK36-Mediated Carbapenem Resistance Attenuates ST258 Klebsiella Pneumoniae in Vivo. Nat. Commun. 2019, 10, 3957. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Papagiannitsis, C.C.; Loli, A.; Tzouvelekis, L.S.; Tzelepi, E.; Arlet, G.; Miriagou, V. SCO-1, a Novel Plasmid-Mediated Class A β-Lactamase with Carbenicillinase Characteristics from Escherichia Coli. Antimicrob. Agents Chemother. 2007, 51, 2185–2188. [Google Scholar] [CrossRef] [Green Version]
  20. Wyres, K.L.; Wick, R.R.; Judd, L.M.; Froumine, R.; Tokolyi, A.; Gorrie, C.L.; Lam, M.M.; Duchene, S.; Jenney, A.; Holt, E. Distinct Evolutionary Dynamics of Horizontal Gene Transfer in Drug Resistant and Virulent Clones of Klebsiella Pneumoniae. PLoS Genet. 2019, 15, e100811. [Google Scholar] [CrossRef] [Green Version]
  21. Turton, J.F.; Payne, Z.; Coward, A.; Hopkins, K.L.; Turton, J.A.; Doumith, M.; Woodford, N. Virulence Genes in Isolates of Klebsiella Pneumoniae from the UK during 2016, Including among Carbapenemase Gene-Positive Hypervirulent K1-St23 and ‘Non-Hypervirulent’ Types ST147, ST15 and ST383. J. Med. Microbiol. 2018, 67, 118–128. [Google Scholar] [CrossRef]
  22. Edward, E.A.; Mohamed, N.M.; Zakaria, A.S. Whole Genome Characterization of the High-Risk Clone ST383 Klebsiella Pneumoniae with a Simultaneous Carriage of BlaCTX-M-14 on IncLM Plasmid and BlaCTX-M-15 on Convergent IncHI1BIncFIB Plasmid from Egypt. Microorganisms 2022, 10, 1097. [Google Scholar] [CrossRef]
  23. Adam, M.A.; Elhag, W.I. Prevalence of Metallo-β-Lactamase Acquired Genes among Carbapenems Susceptible and Resistant Gram-Negative Clinical Isolates Using Multiplex PCR, Khartoum Hospitals, Khartoum Sudan. BMC Infect. Dis. 2018, 18, 4–9. [Google Scholar] [CrossRef]
  24. Al-Hassan, L.; Elbadawi, H.; Osman, E.; Ali, S.; Elhag, K.; Cantillon, D.; Wille, J.; Seifert, H.; Higgins, P.G. Molecular Epidemiology of Carbapenem-Resistant Acinetobacter Baumannii From Khartoum State, Sudan. Front. Microbiol. 2021, 12, 628736. [Google Scholar] [CrossRef]
  25. Afolayan, A.O.; Oaikhena, A.O.; Aboderin, A.O.; Olabisi, O.F.; Amupitan, A.A.; Abiri, O.V.; Ogunleye, V.O.; Odih, E.E.; Adeyemo, A.T.; Adeyemo, A.T.; et al. Clones and Clusters of Antimicrobial-Resistant Klebsiella from Southwestern Nigeria. Clin. Infect. Dis. 2021, 73, S308–S315. [Google Scholar] [CrossRef] [PubMed]
  26. Sherif, M.; Palmieri, M.; Mirande, C.; El-Mahallawy, H.; Rashed, H.G.; Abd-El-Reheem, F.; El-Manakhly, A.R.; Abdel-latif, R.A.R.; Aboulela, A.G.; Saeed, L.Y.; et al. Whole-Genome Sequencing of Egyptian Multidrug-Resistant Klebsiella Pneumoniae Isolates: A Multi-Center Pilot Study. Eur. J. Clin. Microbiol. Infect. Dis. 2021, 40, 1451–1460. [Google Scholar] [CrossRef] [PubMed]
  27. Mohamed, S.B.; Kambal, S.; Munir, A.; Abdalla, N.; Hassan, M.; Hamad, A.; Mohammed, S.; Ahmed, F.; Hamid, O.; Ismail, A.; et al. First Whole-Genome Sequence of a Highly Resistant Klebsiella Pneumoniae Sequence Type 14 Strain Isolated from Sudan. Microbiol. Resour. Announc. 2019, 8, 14–15. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  28. Osman, E.A.; El-Amin, N.; Adrees, E.A.E.; Al-Hassan, L.; Mukhtar, M. Comparing Conventional, Biochemical and Genotypic Methods for Accurate Identification of Klebsiella Pneumoniae in Sudan. Access Microbiol. 2020, 2, e000096. [Google Scholar] [CrossRef] [PubMed]
  29. Higgins, P.G.; Prior, K.; Harmsen, D.; Seifert, H. Development and Evaluation of a Core Genome Multilocus Typing Scheme for Whole-Genome Sequence-Based Typing of Acinetobacter Baumannii. PLoS ONE 2017, 12, e0179228. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  30. Lam, M.M.C.; Wick, R.R.; Watts, S.C.; Cerdeira, L.T.; Wyres, K.L.; Holt, K.E. A Genomic Surveillance Framework and Genotyping Tool for Klebsiella pneumoniae and Its Related Species Complex. Nat. Commun. 2021, 12, 4188. [Google Scholar] [CrossRef]
  31. Zankari, E.; Hasman, H.; Cosentino, S.; Vestergaard, M.; Rasmussen, S.; Lund, O.; Aarestrup, F.M.; Larsen, M.V. Identification of Acquired Antimicrobial Resistance Genes. J. Antimicrob. Chemother. 2012, 67, 2640–2644. [Google Scholar] [CrossRef]
  32. Performance Standards for Antimicrobial Susceptibility Testing An Informational Supplement for Global Application Developed through the Clinical and Laboratory Standards Institute, 26th ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2016; Volume M100S, ISBN 610.688.0700.
  33. EUCAST. EUCAST Reading Guide for Broth Microdilution; EUCAST: Växjö, Sweden, 2020. [Google Scholar]
  34. Aanensen, D.M.; Carlos, C.C.; Donado-Godoy, P.; Okeke, I.N.; Ravikumar, K.L.; Abudahab, K.; Abrudan, M.; Argimón, S.; Harste, H.; Kekre, M.; et al. Implementing Whole-Genome Sequencing for Ongoing Surveillance of Antimicrobial Resistance: Exemplifying Insights into Klebsiella Pneumoniae. Clin. Infect. Dis. 2021, 73, S255–S257. [Google Scholar] [CrossRef]
Antibiotics 12 00233 g001 550
Figure 1. Details of isolate sources and hospitals.
Figure 1. Details of isolate sources and hospitals.
Antibiotics 12 00233 g001
Antibiotics 12 00233 g002 550
Figure 2. Ridom SeqSphere+ minimum spanning tree (MST) for 84 samples based on 2358 columns, pairwise ignoring missing values, logarithmic scale, K. pneumoniae MLST Pasteur (7). Cluster distance threshold: 15. Isolates are grouped by colour, indicating the different STs. Thirty-seven different STs were identified, in addition to 14 transmission clusters, represented by shaded nodes and arrows. Numbers between the nodes indicate the number of allelic differences.
Figure 2. Ridom SeqSphere+ minimum spanning tree (MST) for 84 samples based on 2358 columns, pairwise ignoring missing values, logarithmic scale, K. pneumoniae MLST Pasteur (7). Cluster distance threshold: 15. Isolates are grouped by colour, indicating the different STs. Thirty-seven different STs were identified, in addition to 14 transmission clusters, represented by shaded nodes and arrows. Numbers between the nodes indicate the number of allelic differences.
Antibiotics 12 00233 g002
Table
Table 1. Isolate details: date and type of sample, and hospital.
Table 1. Isolate details: date and type of sample, and hospital.
Sample NameCollection DateHospitalIsolation SourceAdditional Information
HishK12016RUHUrine
HishK22016RUHBloodNICU
HishK102018SUHBloodNICU
HishK32018SUHUrine
HishK42018SUHUrine
HishK52018SUHBloodNICU
HishK72018SUHBloodNICU
HishK82018SUHBloodNICU
HishK92018SUHWound swab
K102018ABHWound swab
HishK112019ASHBlood
HishK122019ASHBlood
HishK132019ASHBlood
HishK142019ASHBlood
K112019ABHUrine
K122019ABHUrine
K132019ABHWound swab
K152019ABHUrine
K22019ABHUrine
K32019ABHBloodNICU
K42019ABHUrine
K52019ABHUrine
K62019ABHBlood
K82019ABHUrine
K92019ABHUrine
LH_R14608 February 2020RUHUrine
LH_S2523 February 2020SUHBlood
LH_R10026 February 2020RUHSputumICU
LH_R9228 February 2020RUHUrine
LH_R10701 March 2020RUHUrineNICU
LH_R12003 March 2020RUHUrine
LH_R15411 March 2020RUHUrineOutpatient
LH_R16718 March 2020RUHBloodNICU
LH_R18219 March 2020RUHBloodInpatient
LH_R19522 March 2020RUHBloodICU
LH_R16423 March 2020RUHSputumInpatient
LH_R17423 March 2020RUHWound swabInpatient
LH_R16223 March 2020RUHUrineOutpatient
LH_R20826 March 2020RUHUrineOutpatient
LH_R22328 March 2020RUHWound swabOutpatient
LH_R21928 March 2020RUHUrineOutpatient
LH_R27512 July 2020RUHUrineInpatient
LH_R28918 July 2020RUHBloodOutpatient
LH_R29027 July 2020RUHUrineUrology unit
LH_R31430 July 2020RUHBlood
LH_R31306 August 2020RUHUrine
LH_R32308 August 2020RUHUrine
LH_R34412 August 2020RUHUrine
LH_F216 August 2020FHUrine
LH_F1516 August 2020FHSputum
LH_F1817 August 2020FHSputum
LH_F2517 August 2020FHBlood
LH_F50_118 August 2020FHUrine
LH_F3518 August 2020FHUrine
LH_F6820 August 2020FHWound swab
LH_F6420 August 2020FHWound swab
LH_F6620 August 2020FHUrine
LH_F8221 August 2020FHPus
LH_F8621 August 2020FHUrine
LH_F9722 August 2020FHUrine
LH_F10122 August 2020FHUrine
LH_R38422 August 2020RUHUrine
LH_F10423 August 2020FHUrine
LH_F10223 August 2020FHBlood
LH_F12225 August 2020FHBlood
LH_F12625 August 2020FHWound swab
LH_F13926 August 2020FHWound swab
LH_F13426 August 2020FHBlood
LH_F13727 August 2020FHSputum
LH_R38727 August 2020RUHUrine
LH_F14327 August 2020FHBlood
LH_F14628 August 2020FHUrine
LH_F14929 August 2020FHPus
LH_F16401 September 2020FHWound swab
LH_F15801 September 2020FHBlood
LH_F15901 September 2020FHBlood
LH_F16902 September 2020FHBlood
LH_F17402 September 2020FHWound swab
LH_F17503 September 2020FHWound swab
LH_F17603 September 2020FHSputum
LH_F19005 September 2020FHSputum
LH_F19205 September 2020FHSputum
LH_F28120 September 2020FHUrine
RUH: Ribat University Hospital, SUH: Soba University Hospital, ASH: Al-Saha Hospital, FH: Fedail Hospital, ABH: Al-Baraha Hospital, NICU: neonatal intensive care unit.
Table
Table 2. Resistome and Virulence details of STs.
Table 2. Resistome and Virulence details of STs.
STESBL
bla		Carb R
bla		Intrinsic β-Lactamase
bla		Virulence
101 (6)CTX-M-15 (6)NDM-1 (1); ompK substitution (1)SHV-1ybt9 (6)
11 (1)CTX-M-15NDM-4SHV-11ybt9
1198 (1)--SHV-11
13 (1)CTX-M-15-SHV-1
1147 (1)--SHV-27
147 (9)CTX-M-15 (9)NDM-1 (5); NDM-5 (1)SHV-11ybt9 (6)
ST15 (6)CTX-M-15 (5)OmpK35 substitution (1)SHV-28ybt9 (1)
ST17 (4)CTX-M-15 (2)NDM-1SHV-11
ST20 (7)CTX-M-15 (7)NDM-1 (5)SHV-187
ST218-3LV--SHV-93
ST219 (2)CTX-M-15 (2)-SHV-1
ST231 (2)CTX-M-15 (1)OXA-232 (1)SHV-1ybt14 (1); iuc (1)
ST237 (2)--SHV-11
ST24-1LV--SHV-11
ST2459 (1)CTX-M-15-SHV-1
ST2674 (1)CTX-M-15NDM-1SHV-11
ST2735 (1)--SHV-11
ST29-1LV (1)CTX-M-15--
ST292 (1)CTX-M-15-SHV-11
ST307 (6)CTX-M-15 (6)NDM-1 (4)SHV-28ybt10 (3)
ST3161 (1)--SHV-11
ST3430 (1)CTX-M-15-SHV-77
ST38 (1)CTX-M-15NDM-1SHV-11
ST383 (5)CTX-M-14 (4);CTX-M-15 (4)NDM-5 (4); OXA-48 (4)SHV-1iuc1 (4); rmp1 (2)
ST39 (1)--SHV-1ybt4
ST437 (7)CTX-M-15 (7)NDM-1 (1); NDM-5 (6)SHV-11ybt9 (7)
ST45 (1)CTX-M-15OmpK36 variantSHV-1ybt10
ST469 (1)CTX-M-15-SHV-11
ST474 (1)CTX-M-15-SHV-11
ST501 (1)CTX-M-15-SHV-11
ST514 (1)CTX-M-15-SHV-63
ST530 (2)CTX-M-15 (2)NDM-1 (2)-ybt10 (2)
ST664 (2)CTM-X-15 (2)NDM-1 (2)SHV-11
ST882 (2)---ybt (2)
ST901 (1)CTX-M-15-SHV-1
ST: sequence type; ESBL: extended-spectrum β lactamase; Carb: carbapanemase; OmpK: outer-membrane porin substitutions contributing to carbapenem resistance; ybt: Yersiniabectin; Numbers in brackets indicate the number of isolates. Table S1 contains details of each isolate.
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Osman, E.A.; Yokoyama, M.; Altayb, H.N.; Cantillon, D.; Wille, J.; Seifert, H.; Higgins, P.G.; Al-Hassan, L. Klebsiella pneumonia in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence. Antibiotics 2023, 12, 233. https://doi.org/10.3390/antibiotics12020233

AMA Style

Osman EA, Yokoyama M, Altayb HN, Cantillon D, Wille J, Seifert H, Higgins PG, Al-Hassan L. Klebsiella pneumonia in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence. Antibiotics. 2023; 12(2):233. https://doi.org/10.3390/antibiotics12020233

Chicago/Turabian Style

Osman, Einas A., Maho Yokoyama, Hisham N. Altayb, Daire Cantillon, Julia Wille, Harald Seifert, Paul G. Higgins, and Leena Al-Hassan. 2023. "Klebsiella pneumonia in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence" Antibiotics 12, no. 2: 233. https://doi.org/10.3390/antibiotics12020233

APA Style

Osman, E. A., Yokoyama, M., Altayb, H. N., Cantillon, D., Wille, J., Seifert, H., Higgins, P. G., & Al-Hassan, L. (2023). Klebsiella pneumonia in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence. Antibiotics, 12(2), 233. https://doi.org/10.3390/antibiotics12020233

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