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Volume 77
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10.3797/scipharm.0908-03
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Article

An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.)

by
Christoph WAWROSCH
*,
Eva BENDA
and
Brigitte KOPP
Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090, Vienna, Austria
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2009, 77(4), 827-836; https://doi.org/10.3797/scipharm.0908-03
Submission received: 4 August 2009 / Accepted: 21 September 2009 / Published: 21 September 2009
Versions Notes

Abstract

An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with a 5-weeks main culture without plant growth regulators (step 2: shoot elongation). Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up.
Keywords: Endangered; Medicinal Plant; Liquid Medium; Micropropagation Endangered; Medicinal Plant; Liquid Medium; Micropropagation

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MDPI and ACS Style

WAWROSCH, C.; BENDA, E.; KOPP, B. An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.). Sci. Pharm. 2009, 77, 827-836. https://doi.org/10.3797/scipharm.0908-03

AMA Style

WAWROSCH C, BENDA E, KOPP B. An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.). Scientia Pharmaceutica. 2009; 77(4):827-836. https://doi.org/10.3797/scipharm.0908-03

Chicago/Turabian Style

WAWROSCH, Christoph, Eva BENDA, and Brigitte KOPP. 2009. "An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.)" Scientia Pharmaceutica 77, no. 4: 827-836. https://doi.org/10.3797/scipharm.0908-03

APA Style

WAWROSCH, C., BENDA, E., & KOPP, B. (2009). An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.). Scientia Pharmaceutica, 77(4), 827-836. https://doi.org/10.3797/scipharm.0908-03

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