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Article

Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora

by
Mila Marinovíc
1,†,
Marcos Di Falco
2,†,
Maria Victoria Aguilar Pontes
3,‡,
András Gorzsás
4,
Adrian Tsang
2,
Ronald P. de Vries
3,
Miia R. Mäkelä
1 and
Kristiina Hildén
1,*
1
Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00790 Helsinki, Finland
2
Centre for Structural and Functional Genomics, Concordia University, Montréal, QC H4B 1R6, Canada
3
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
4
Department of Chemistry, Umeå University, 901 87 Umeå, Sweden
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
Current address: Departamento de Genética, Universidad de Córdoba, Campus de Rabanales Córdoba, C5, 14071 Villanueva del Rey, Spain.
Biomolecules 2022, 12(8), 1017; https://doi.org/10.3390/biom12081017
Submission received: 31 May 2022 / Revised: 8 July 2022 / Accepted: 20 July 2022 / Published: 22 July 2022
(This article belongs to the Special Issue Regulation and Mechanisms of Plant Biomass Degrading Enzymes from Fungi)
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Abstract

:
The unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed the proteomes of two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, during eight-week cultivation on solid spruce wood. Plant cell wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% of the total proteins in both species. A core set of orthologous plant cell wall degrading CAZymes was shared between these species on spruce suggesting a conserved plant biomass degradation approach in this clade of basidiomycete fungi. However, differences in time-dependent production of plant cell wall degrading enzymes may be due to differences among initial growth rates of these species on solid spruce wood. The obtained results provide insight into specific enzymes and enzyme sets that are produced during the degradation of solid spruce wood in these fungi. These findings expand the knowledge on enzyme production in nature-mimicking conditions and may contribute to the exploitation of white rot fungi and their enzymes for biotechnological applications.

1. Introduction

Wood degradation by fungi is a significant process in terrestrial environments with respect to the cycling of carbon and other nutrients [1,2]. The wood cell walls are composed of the three main polysaccharide components: cellulose, hemicellulose, and pectin, as well as the aromatic polymer lignin. While many fungi can degrade wood cell wall polysaccharides, only basidiomycetous white rot fungi can efficiently degrade lignin [3]. This is due to their unique ability to produce extracellular lignin modifying peroxidases, which catalyze unspecific oxidative reactions to degrade the heterogeneous lignin structures. Moreover, white rot fungal genomes possess several hydrogen peroxide-producing enzymes needed for the activity of the lignin modifying peroxidases, in addition to a wide array of carbohydrate active enzymes (CAZymes) targeted to lignocellulosic polysaccharides (www.cazy.org) that also is produced by a broader range of fungi [4].
Two types of wood decay patterns have been suggested among white rot fungi. In the so-called simultaneous degradation, lignin and (hemi)cellulose are depolymerized at the same time, as exemplified by the action of the well-studied white rot fungus Phanerochaete chrysosporium [5]. In contrast, some white rot species, such as Gelatoporia (Ceriporiopsis) subvermispora and Obba rivulosa, have been suggested to cause selective lignin degradation by preferential removal of lignin before wood polysaccharides [6,7,8]. Selective lignin degradation is typically observed in the early stages of fungal growth, but physical and chemical conditions of the substrate, such as temperature, moisture, and nitrogen and oxygen content, also affect the selectivity of lignin degradation [9,10]. While definitive enzymatic mechanisms behind the selective degradation remain uncertain, increased oxidoreductase and diminished cellulolytic potential have been suggested to differentiate selective delignifiers from species that simultaneously degrade all lignocellulose polymers [11].
Gelatoporia subvermispora and O. rivulosa are closely related species from the Gelatoporia clade, which is an evolutionarily distinctive group within the order Polyporales, comprising a small group of white rot wood-inhabiting fungi [12,13]. Genome content in terms of putative plant cell wall degrading enzyme (PCWDE) encoding genes suggests that G. subvermispora and O. rivulosa have similar potential for lignocellulose decay [11,14,15].
Orthologs are homologous genes or proteins that are shared among related species. They are derived from a common ancestral gene that has diverged after the speciation event but with retention of similar biological function [16]. Ortholog analysis is a useful tool in comparative proteomic studies because it can highlight genes shared among species that are important for conserved biological processes. It also can reveal which genes are unique for a particular subset of fungi, such as fungi with a specific lifestyle. As an example, closely related ascomycete species from the genus Aspergillus with highly similar genomic potential have been shown to markedly differ in their enzymatic approaches for plant biomass degradation by producing nonorthologous CAZymes [17].
In this study, we wanted to test whether this also is true for closely related wood degrading white rot fungi. For this, we explored the proteomes of the genome-sequenced monokaryotic strains of G. subvermispora RP-B and O. rivulosa 3A-2 [11,14] during an 8-week cultivation on solid spruce wood sticks, thus mimicking the environmental conditions these fungi encounter in their natural habitat. Temporal production of plant cell wall targeted CAZymes was detected by LC-MS/MS and a comparative analysis of CAZyme orthologs in O. rivulosa and G. subvermispora was performed to evaluate how similar enzymatic machineries these related white rot fungi employ for spruce wood degradation.

2. Materials and Methods

2.1. Fungal Strains and Cultivation Conditions

Obba rivulosa monokaryon 3A-2 (FBCC1032), derived from the dikaryotic O. rivulosa strain T241i (FBCC939), was obtained from the HAMBI Fungal Biotechnology Culture Collection, University of Helsinki, Finland ([email protected]). Gelatoporia (Ceriporiopsis) subvermispora monokaryon FP-105752 (RP-B), derived from the parental dikaryotic strain 105752 [18], was a kind gift from Dr. Dan Cullen, Forest Products Laboratory, Madison, WI, USA.
The fungi were maintained on 2% malt extract agar plates (MEA; 2% (w/v) malt extract, 2% (w/v) agar). Pre-cultivations contained 100 mL low nitrogen–asparagine–succinate (LN-AS) medium, pH 4.5 [19], supplemented with 0.05% glycerol in 250 mL Erlenmeyer flasks, and inoculated with five mycelium-covered agar plugs (Ø 7 mm) for 7 days at 28 °C. Pre-cultivations were homogenized (Waring Blender, Torrington, CT, USA) [20] and solid spruce wood cultivations containing 2 g (dry weight) of Norway spruce (Picea abies) sapwood wood sticks (2 × 0.2 × 0.3 cm) on 1% (w/v) water agar in Erlenmeyer flasks were inoculated with 4 mL of mycelial suspension (Supplementary Figure S1). Moisture content of the cultivation was adjusted to 60%. Cultivations were performed as duplicates and incubated in dark for 2, 4, and 8 weeks at 28 °C.

2.2. Protein Extraction and LC-MS/MS Identification

For proteome analysis, duplicate fungal spruce wood cultures incubated for 2, 4, and 8 weeks were ground (A11 Basic analytical mill, IKA, Merck, Darmstadt, Germany) under liquid nitrogen. The milled cultures were extracted with 40 mL 25 mM potassium phosphate buffer, pH 7.0, under agitation (150 rpm) at 4 °C for 2 h. The extract was filtered (GF/C glass fiber filters, Whatman) and proteins in the liquid fraction were precipitated with 20% trichloroacetic acid (Sigma-Aldrich, St. Louis, MI, USA), 20 mM dithiothreitol (DTT, Sigma Aldrich), and 80% acetone (Sigma-Aldrich) solution, resuspended and reprecipitated with 20 mM DTT and 80% acetone solution as described previously [21].
Ten micrograms of protein were resuspended in 0.25% Anionic Acid Labile Surfactant 26 I (AALS I, ProgentaTM, Protea Biosciences, Inc., Morgantown, WV, USA) detergent prepared in 200 mM ammonium bicarbonate buffer, pH 7.8, loaded and separated on 12% SDS-PAGE gels for subsequent in-gel trypsin digestion as previously described [22]. Protein separation was stopped as soon as the 250 kDa marker could be seen entering the separating gel. The complete lane, delimited by the bromophenol blue front at the bottom and the start of the separating gel at the top (about 1.5 cm), was cut and processed for in-gel digestion as a whole.
In-gel digested peptide extracts from two biological replicate cultures were analyzed on an LTQ-Velos-Orbitrap mass spectrometer (Thermo-Fisher, San Jose, CA, USA), and the acquired MS/MS data were searched against databases at JGI Mycocosm for the O. rivulosa (https://mycocosm.jgi.doe.gov/Obbri1/Obbri1.home.html, accessed 10 July 2015) and G. subvermispora (https://mycocosm.jgi.doe.gov/Cersu1/Cersu1.home.html, accessed 10 July 2015) containing 13,206 and 12,125 predicted protein sequences, respectively, for peptide/protein identification as previously reported [23]. Protein area values were calculated using the top five most abundant identified peptides for each protein determined using the precursor ion quantitation workflow from Proteome Discoverer 2.4 (Thermo-Fisher) and were used to quantify relative levels of total proteins. Protein area values were normalized using the determined value for the spiked trypsin-digested bovine serum albumin internal standard. For each protein, mean abundance was calculated from two biological replicates and expressed as ion profile (IP) value. Proteins with IP value < 5 were filtered out from the analysis. The proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD030609 and 10.6019/PDX030609. Principal component analysis (PCA) of the protein abundances on duplicate samples of the three-time points in two fungal species was performed with FactoMineR v1.7 package from R commander v.2.1-7 program (J. Fox, CRAN mirror in Utrecht, The Netherlands) in R statistical language and environment 3.1.2 [24]. Orthologous groups (OGs) were identified by utilizing OrthoMCL method [25] with parameters set as follows: E-value 1 × 10−5, inflation level 1.5, and sequence coverage 60%.

2.3. FTIR Analysis of Spruce Wood

The pooled samples from duplicate cultivations were analyzed by diffuse reflectance FTIR spectroscopy using a Bruker IFS 66V/S instrument (Bruker Optik GmbH, Ettlingen, Germany) under vacuum (4 mbar) conditions, according to the protocol by Gorzsás and Sundberg (2014) [26]: dried samples (1–10 mg) were mixed with infrared spectroscopy grade potassium bromide (KBr, ca. 390 mg) and manually ground using an agate mortar and pestle to obtain a homogeneous mixture. Spectra were recorded in the range of 400-4000 cm−1 at 4 cm−1 spectral resolution with 128 scans co-added, using pure KBr as background with the same parameters. Spectra were processed using the free, open source, Matlab-based script available from the Vibrational Spectroscopy Core Facility at Umeå University (www.umu.se/en/research/infrastructure/visp/downloads/, accessed on 11 April 2019) [27], using the following steps with the listed parameters: (1) Spectral region cut to 750–1850 cm−1 (fingerprint region); (2) baseline correction using asymmetrical least squares (lambda = 10,000,000, p = 0.001 (minimum); (3) total area normalisation over the cut spectral range; and (4) Savitzky–Golay smoothing (first order polynomial with a frame of three). No derivatisation or any other processing was applied after these steps.

3. Results and Discussion

3.1. Temporal Protein Profiles of O. rivulosa and G. subvermispora during Cultivation on Spruce

Proteomics analysis using LC-MS/MS was performed to investigate the lignocellulolytic protein profiles of O. rivulosa and G. subvermispora during growth on spruce wood sticks for 2, 4, and 8 weeks. The total number of detected proteins from the cultivations of O. rivulosa and G. subvermispora was 947 and 845, respectively (Supplementary Table S1c,f). Signal peptide was predicted for 14% of the proteins in both species, which is in line with the secreted proteins (15%) detected in white rot species Phlebia radiata cultivated on solid spruce wood [28]. Protein abundances from the biological replicate cultivations showed high coherence according to PCA analysis (Supplementary Figure S2). The genomes of both species used in this study harbor a broad set of predicted CAZymes targeted to degrade all polymeric components of wood cell walls. Their gene counts for CAZy family AA2 lignin modifying peroxidases and hydrogen peroxide producing family AA5_1 enzymes are typical for white rot species in Polyporales, while low numbers of laccase family AA1 and lytic polysaccharide monooxygenase (LPMO) family AA9 encoding genes resembled brown rot species in a sister clade Antrodia [29]. Of the detected proteins, 46 and 49 represented putative plant cell wall degrading CAZymes accounting for approximately 5% of the total proteome for both O. rivulosa and G. subvermispora, respectively (Supplementary Table S1a,d). Slightly higher proportion of plant cell wall degrading CAZymes (7%) has been detected in the total proteome of the white rot species Phlebia radiata cultivated on spruce wood [28]. Plant cell wall targeted CAZymes were classified into functional categories based on the substrate(s) on which they (putatively) are active.
Orthologous plant cell wall degrading CAZymes among O. rivulosa and G. subvermispora were identified and pairwise orthology relationship between them was inferred. In total, 64 common orthologous groups, a cluster of homologous sequences that diverged from the same speciation event, were found (Supplementary Table S1i,j). The analysis revealed the presence of orthologs in both proteomes for the majority of PCWDEs, indicating that O. rivulosa and G. subvermispora share a core set of orthologous PCWDEs during wood decay. In contrast, comparison of white and brown rot basidiomycete transcriptomes from various lignocellulose substrates has shown that only a few CAZyme orthologous gene groups are consistently upregulated that indicates diversity in their approaches to degrade plant biomass [30]. In O. rivulosa proteome, 80% of the plant cell wall degrading orthologs also were detected in G. subvermispora, while of the CAZy proteins produced by G. subvermispora, 15% were unique, and their orthologs were not produced by O. rivulosa (Supplementary Table S1i,j). Highly produced orthologs, which were not detected in G. subvermispora, were O. rivulosa LAC (GH35) and EGL (GH131) found at all time points, as well as GH27 (α-galactosidase, AGL), GH51 (α-arabinofuranosidase, ABF) and GH53 (β-endogalactanase, GAL), which were produced in 2-week cultivation of O. rivulosa (Supplementary Table S1i). O. rivulosa GH6 (CBHII) was produced in 2- and 4-week cultivations, whereas no CBHII was detected in G. subvermispora cultivations. In G. subvermispora, two H2O2-generating oxidases in CAZy families AA3_2 (aryl alcohol oxidase, AAO) and AA3_3 (alcohol oxidase, AOX) GH131 (EGL), a xyloglucan-acting GH31 (α-xylosidase, AXL) and CE1 (acetyl xylan esterase, AXE) were produced, but corresponding orthologs were not found in O. rivulosa (Supplementary Table S1j).
A decrease in the number of PCWDEs, from 43 to 32, was detected between the 2- and 8-week spruce cultivations of O. rivulosa (Figure 1a). A similar trend was detected in the transcriptome of O. rivulosa when it was cultivated on spruce wood for 2, 4, and 8 weeks [31]. The highest number of PCWDEs (43) was detected in the 2-week cultures of G. subvermispora, with a clear decreasing trend after 4 and 8 weeks to 38 and 33 CAZymes, respectively (Figure 1b).
More than half of the total number of detected plant cell wall degrading CAZymes, 27 and 30, were produced in all studied time points in the cultivations of O. rivulosa and G. subvermispora, respectively (Figure 2, Supplementary Table S1g,h). In both species, most of these common proteins were cellulases and hemicellulases from diverse GH families. Lignin modification-related enzymes were common to all time points comprised of AA3 H2O2-supplying enzymes in both species, as well as AA2 manganese peroxidases (MnPs) in G. subvermispora. After 2-week cultivation, 11 and 6 CAZymes were exclusive for O. rivulosa and G. subvermispora cultures, respectively (Figure 2). The number of the unique proteins strongly decreased in the later time points of both fungi. None were detected in 4-week cultivations, and only one protein was present in 8-week cultivations of O. rivulosa. In G. subvermispora cultivation, one and four unique proteins were detected in 4- and 8-week cultivations, respectively (Figure 2b). In addition, one O. rivulosa and G. subvermispora PCWDE was shared between 4- and 8-week cultivations. Only O. rivulosa had PCWDEs that were shared between 2- and 8-week cultivations (Figure 2a).

3.2. Early Production of MnPs Suggests Their Important Role during the Beginning of Wood Decay

The number of lignin acting enzymes produced by O. rivulosa decreased during the cultivation, whereas in G. subvermispora the number of detected proteins were constant in all the time points during the 8-week cultivations (Figure 1). Three MnPs (Protein IDs: 727100, 891614, and 438941) out of 11 MnP encoding genes of O. rivulosa (https://genome.jgi.doe.gov/pages/search-for-genes.jsf?organism = Obbri1, accessed 5 April 2022) were produced in 2-week cultivations (Figure 2), which correlates well with our previous transcriptomic study where two MnPs (Prot. IDs: 727100 and 891614) were among the highest expressed PCWDE encoding genes during O. rivulosa cultivation on spruce [31]. O. rivulosa MnP 891614 was the only MnP among the most abundant PCWDEs produced (Table 1). Its production was the highest in 2-week cultivations, and only low amounts were produced at the later stages of cultivation. In G. subvermispora cultivations, MnPs were the most abundant lignin modifying enzymes produced with five detected isoenzymes (Protein IDs: 105539, 94398, 50686, 49863, and 117436) out of 13 MnP encoding genes (Table 2) [11]. Three highly produced MnPs showed a decreasing trend over time, similarly with a majority of the ten detected MnPs in the secretome of G. subvermispora (strain RP-B) grown in the ball-milled aspen containing liquid cultures, thus highlighting the importance of these enzymes in the early stages of wood degradation [32]. The most highly produced was MnP7 (Protein ID: 105539), which was detected throughout the cultivation, with an exceptionally high abundance in 8-week cultivations (Table 2). The amount of MnP10 (Protein ID: 117436) slightly increased during cultivation. Previously, MnP10 had been reported to be the most strongly upregulated MnP in ball-milled aspen containing liquid cultivations of G. subvermispora during seven days of cultivation [32]. As significant lignin loss in solid spruce wood has been detected after three days of G. subvermispora cultivation, selective lignin degradation is pronounced in very early stages of wood decay [33].
This is supported by the spruce wood composition analyses after fungal growth by FTIR (Figure 3). Relative lignin/carbohydrate ratio was determined as the intensities of 1510 cm−1/1060 cm−1 [34] indicated loss in lignin in the early stages of G. subvermispora and O. rivulosa cultivation on spruce. G. subvermispora is more efficient than O. rivulosa in lignin degradation after two weeks of cultivation, yet the ratio is similar for both species after eight weeks. Similarly, degradation of lignin and hemicelluloses by G. subvermispora has been detected on oak wood during the first five weeks of cultivation [35].
In addition to MnPs, O. rivulosa and G. subvermispora encode one and two lignin peroxidase-type enzymes, respectively [14,18]. However, none were produced during 8-week cultivation on spruce. Detection of MnPs as the only lignin modifying peroxidases in O. rivulosa and G. subvermispora cultivations emphasized their importance in degradation of solid spruce wood. This is consistent with the previous studies on the production of MnPs as the main lignin modifying enzymes of O. rivulosa when grown on spruce wood chips [36,37]. Likewise, G. subvermispora (strain SS-3) has been shown to produce MnP as its main lignin modifying enzyme on loblolly pine and eucalyptus wood chips [38,39].
Besides lignin modifying peroxidases, laccases also are implicated in lignin degradation. Oxidation of wood lignin structures has been detected by fungal laccases either alone or in the presence of small molecular weight redox mediators [40,41,42]. Eight laccases are present in the genome of O. rivulosa [14], while G. subvermispora has seven predicted laccase encoding genes [11]. Unlike multiple MnP isoenzymes detected in the studied spruce stick cultures, only a single laccase isoenzyme was produced by O. rivulosa (Protein ID: 452017) after 2 and 4 weeks, and the corresponding laccase ortholog of G. subvermispora (Protein ID: 118801) was produced after 2 weeks. In addition, this laccase isoenzyme was the only lignin modifying enzyme produced by G. subvermispora after 2-week cultivation (Figure 2b). Low abundances and secretion limited to the early cultivation stage indicate that laccases may have restricted influence on lignin degradation in O. rivulosa and G. subvermispora, but they may have a role in initial wood colonization in natural conditions. Correspondingly, we have detected low and early expression of laccase-encoding genes in O. rivulosa spruce stick cultivations [31].
In the lignin degradation process by white rot fungi, an array of redox enzymes, such as family AA3 glucose–methanol–choline (GMC) oxidoreductases and AA5 copper radical oxidases (CROs), supplies H2O2 to class II peroxidases [43] as well as to LPMOs [44]. H2O2-supplying enzymes represented a small proportion of the plant cell wall degrading CAZymes in O. rivulosa (4%), whereas in G. subvermispora they constituted a notably higher fraction making 10% of detected PCWDEs. H2O2-generating family AA3_3 AOX enzymes were the most highly produced PCWDEs in O. rivulosa and G. subvermispora spruce cultures, thus indicating a very potent ligninolytic system in both species studied (Table 1 and Table 2). The highly produced G. subvermispora AOX (Protein ID: 80773) also has been abundantly produced in solid-state fermentation (SSF) of Jerusalem artichoke, together with an AA3_2 aryl alcohol oxidase (AAO) (Protein ID: 117387) [45]. Interestingly, this AAO has been the main H2O2-supplying enzyme in the secretome of G. subvermispora liquid ball-milled aspen cultures [32], but it was not detected in our study.

3.3. O. rivulosa and G. subvermispora Showed Constant Production of Cellulolytic Enzymes

Cellulose depolymerizing enzymes comprised the largest proportion of plant cell wall degrading CAZymes produced by O. rivulosa (23%) (Supplementary Table S1a). The number of cellulolytic enzymes stayed constant in both O. rivulosa and G. subvermispora cultures during 8-week cultivation (Figure 1).
A full enzymatic repertoire necessary for cellulose hydrolysis was produced by both species studied. The most highly produced cellulolytic enzymes in both species were GH7 cellobiohydrolase I (CBHI) targeted toward degradation of crystalline cellulose (Table 1 and Table 2). Interestingly, O. rivulosa and G. subvermispora produced the highest levels of CBHIs and other cellulases after 2 weeks, similar to lignin modifying enzymes, disputing the hypothesized selective lignin degradation approach in these conditions. Previously, minor depolymerization of cellulose had been detected in G. subvermispora (strain SS-3) eucalyptus wood chip cultures already 15 days of cultivation [46], which supports our finding of an active cellulolytic capacity. A repertoire of cellulose hydrolyzing enzymes was complemented by EGLs and BGLs, as well as oxidative LPMOs cleaving cellulose and other plant cell wall polysaccharides, and a cellobiose dehydrogenase (CDH) that serves as an external electron donor in LPMO-catalyzed reactions [47,48] (Table 1), which were present in both fungal proteomes grown on spruce wood. Both O. rivulosa and G. subvermispora with eight and nine putative AA9 LPMO encoding genes, respectively, indicate oxidative capacity for the degradation of plant polysaccharides. Two O. rivulosa LPMOs were among the most abundantly produced proteins in the spruce cultures, which is in line with the high transcript levels of their corresponding genes under similar cultivations [31]. All three G. subvermispora LPMOs detected from spruce cultivations in this study also have been upregulated at the transcript level on liquid ball-milled aspen medium; but surprisingly, none of those have been detected as proteins from liquid ball-milled aspen, Avicel or glucose cultivations [11]. Levels and production trends of CDH were in accordance with those of the LPMOs in both studied species. This indicates a certain discrepancy between LPMO expression and production levels. Inconsistencies between transcriptome and proteome data in fungal cultures have been reported, and they have been suggested to result from differential transcription and translation rate, mRNA, and protein stability as well as biochemical properties of proteins [21,49,50].

3.4. Early Production of Hemicellulases Coincided with Lignin Modifying Enzymes

Xylanases accounted for the majority (28%) of the detected PCWDEs in G. subvermispora spruce cultivations (Figure 1b), while a smaller portion was observed in O. rivulosa cultivations (18%). Family GH5_7 mannan hydrolyzing β-1,4-endomannanase (MAN) (Protein ID: 641261) was the most abundant hemicellulase in the O. rivulosa spruce cultivations, indicating that this fungus has a good ability to convert spruce that has mannan as the main hemicellulose polymer [51]. We previously showed that GH5_7 MAN and GH10 β–1,4-endoxylanase (XLN) encoding genes were expressed at comparably high levels in the O. rivulosa spruce cultures after two weeks [31]. Although xylan is present in lower amounts in spruce (12.4 mol%) compared to mannan (16.7 mol%) [51], GH10 XLNs, which hydrolyzes β-1,4-bonds in the xylan backbone, were the main hydrolytic enzymes produced by G. subvermispora with all its six XLN isoenzymes detected. The two most highly produced XLNs on spruce wood (Protein IDs: 59733, 97858) also were detected from the ball-milled aspen liquid cultivations of G. subvermispora [32], whereas two different isoenzymes (Protein IDs: 67561, 116326) were identified in the exo-proteome of G. subvermispora from Jerusalem artichoke SSF cultivations [45], implying induction of different isoenzymes by different plant biomass substrates.
A relatively low presence of xyloglucanases, representing approximately 5% of the detected PCWDEs, was detected in both species. This reflects the low content of xyloglucan in softwood (10% (w/w) in the primary cell walls) [52]. Most of the hemicellulases in both species showed decreasing levels over the cultivation time, similarly as lignin modifying enzymes, thus supporting a simultaneous lignin degrading approach rather than a predicted selective lignin removal. The exceptions were two detected GH2 MNDs and two intracellular GH5_22 BXLs in O. rivulosa cultivations, and two GH10 XLNs and one CE15 glucuronoyl esterase (GE) in G. subvermispora cultivations, all of which were most abundant after 8 weeks (Supplementary Table S1d).
Diverse activities comprising enzymes, such as GH51 ABFs, CE16 hemicellulose acetyl esterases (HAEs), GH35 β-galactosidases (LACs), and AA9 LPMOs, putatively acting on several substrates, constituted 22% of all detected CAZymes in O. rivulosa and 18% in G. subvermispora (Supplementary Table S1b,e). The number of CAZymes with diverse activities decreased in the cultures of O. rivulosa, while in G. subvermispora the number stayed constant during the whole cultivation period (Figure 1). Interestingly, HAE was one of the most abundantly produced enzymes in O. rivulosa, while in G. subvermispora LPMOs were the most abundant diverse activities comprising enzymes.
GH51 ABFs act on side-chains of different arabinose-containing polysaccharides, such as xyloglucans, arabinoxylans, and pectin [53]. Although arabinose is only a minor constituent in wood accounting for 2.9 mol% in spruce [51], interestingly, one ABF isoenzyme was detected in the spruce cultivations of both species. The same G. subvermispora ABF (Protein ID: 162360) has been detected from SSF cultivation on Jerusalem artichoke that has higher arabinose content (5%) [45] as well as from the submerged ball-milled aspen cultures at the initial cultivation stage [32]. ABF has been speculated to indirectly contribute to lignin degradation; [32] although, there is no evidence of the existence of ferulic acid-linked arabinose in wood, which could be cross-linked to lignin.

4. Conclusions

In conclusion, both O. rivulosa and G. subvermispora produced all the necessary lignocellulolytic enzymes for the white rot type of decay of solid spruce wood, which involved an array of secreted cellulases, hemicellulases, lignin modifying enzymes, and auxiliary oxidoreductases. A core set of orthologous PCWDEs was shared suggesting similar approach for plant biomass degradation. Dynamics of the detected protein levels during the 8-week cultivation revealed that several lignin modifying class II peroxidases together with other oxidoreductases were abundantly produced after 2-week cultivation, suggesting an oxidative attack on lignin during the early growth stage on wood. Similar trends in abundances of class II peroxidases have previously been noticed for O. rivulosa [36], G. subvermispora [45], and other white rot species grown on solid lignocellulose substrates, such as Phlebia radiata [28].
Although selective lignin degradation has been postulated for O. rivulosa and G. subvermispora, in the conditions used in this study the obtained results did not corroborate these monokaryotic strains as selective lignin degraders. While enzymatic mechanisms behind selective lignin degradation remain still largely unsolved, it is known that this strategy can be affected by multiple parameters. One of the most prominent factors is temporal dependency, which limits lignin removal to an early decay stage, whereas hemicellulose and cellulose degradation appear later [8,54]. In addition, just like various strains of a species are known to show considerable variations in lignin selectivity, there are differences in degradation pattern between dikaryotic and monokaryotic strains of the same species [55,56,57]. Higher mycelial growth rate, enzyme activity levels, and broader induction of CAZyme encoding genes of dikaryotic strains compared to the monokaryotic strains, indicate an overall better ability to grow on and utilize plant biomass. Monokaryotic O. rivulosa and G. subvermispora showed that even though the majority of CAZymes that they produced during growth on spruce were orthologs, their diversity with respect to time-dependent production of plant cell wall degrading enzymes suggests a different strategy for spruce degradation for these taxonomically related species.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/biom12081017/s1, Figure S1: Fungal growth on spruce wood sticks for 2, 4, and 8 weeks. (a) O. rivulosa monokaryon 3A-2 and (b) G. subvermispora monokaryon RP-B; Figure S2: Principal component analysis (PCA) of the proteomes of (a) O. rivulosa and (b) G. subvermispora. Table S1: Plant cell wall degrading CAZymes and their respective orthologs detected in the proteome of O. rivulosa and G. subvermispora.

Author Contributions

Conceptualization, K.H., M.R.M. and R.P.d.V.; methodology and validation, M.M., M.D.F. and A.G.; formal analysis and investigation, M.M., M.D.F., M.V.A.P. and A.G.; resources, A.T., A.G. and K.H.; data curation, M.D.F.; writing—original draft preparation, M.M., M.D.F. and A.G.; writing—review and editing, K.H., M.R.M. and R.P.d.V.; visualization, M.M. and K.H.; supervision, K.H., M.R.M. and R.P.d.V.; project administration and funding acquisition, K.H. All authors have read and agreed to the published version of the manuscript.

Funding

M.M. was supported by the European Commission, Marie Curie ITN network SuBiCat FP7 Grant agreement no: 607044 and the Academy of Finland research grant no: 297847. The Academy of Finland grant no: 308284 to MRM is acknowledged. M.V.A.P. was supported by a grant of the Dutch Technology Foundation STW, Applied Science division of NWO; and the Technology Program of the Dutch Ministry of Economic Affairs 016.130.609 to RPdV. Mass spectrometry analysis was performed at the Centre for Biological Applications of Mass Spectrometry (CBAMS) at Concordia University, Montreal, Canada.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (www.ebi.ac.uk/pride/) and are accessible with the data set identifier PXD030609 and 10.6019/PDX030609.

Acknowledgments

Dan Cullen (Forest Products Laboratory, Madison, WI, USA) is acknowledged by providing us with G. subvermispora monokaryon FP-105752 (RP-B).

Conflicts of Interest

The authors declare no conflict of interest.

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Biomolecules 12 01017 g001 550
Figure 1. Functional distribution of detected predicted plant cell wall degrading CAZymes in spruce wood cultures of (a) O. rivulosa and (b) G. subvermispora on week 2, 4, and 8. Numbers in the center of the circles show the sum of detected plant cell wall degrading CAZymes in the specific time point. Numbers outside the circles display detected PCWDEs acting on their (putative) substrates at each time point.
Figure 1. Functional distribution of detected predicted plant cell wall degrading CAZymes in spruce wood cultures of (a) O. rivulosa and (b) G. subvermispora on week 2, 4, and 8. Numbers in the center of the circles show the sum of detected plant cell wall degrading CAZymes in the specific time point. Numbers outside the circles display detected PCWDEs acting on their (putative) substrates at each time point.
Biomolecules 12 01017 g001
Biomolecules 12 01017 g002 550
Figure 2. Venn diagrams showing the unique and common plant cell wall acting CAZymes in 2-, 4-, and 8-week solid spruce wood cultivations of (a) O. rivulosa and (b) G. subvermispora. CAZyme abbreviations (see Supplementary Table S1a,d) followed by JGI Protein IDs are depicted, and their font color is according to the substrate they are putatively acting on.
Figure 2. Venn diagrams showing the unique and common plant cell wall acting CAZymes in 2-, 4-, and 8-week solid spruce wood cultivations of (a) O. rivulosa and (b) G. subvermispora. CAZyme abbreviations (see Supplementary Table S1a,d) followed by JGI Protein IDs are depicted, and their font color is according to the substrate they are putatively acting on.
Biomolecules 12 01017 g002
Biomolecules 12 01017 g003 550
Figure 3. Diffuse reflectance FTIR spectra of Norway spruce wood samples after 2, 4, and 8 weeks of fungal cultivation: (a) FTIR spectra after O. rivulosa cultivation, (b) FTIR spectra after G. subvermispora cultivation, and (c) lignin (1510 cm−1)/polysaccharide (1060 cm−1) ratio.
Figure 3. Diffuse reflectance FTIR spectra of Norway spruce wood samples after 2, 4, and 8 weeks of fungal cultivation: (a) FTIR spectra after O. rivulosa cultivation, (b) FTIR spectra after G. subvermispora cultivation, and (c) lignin (1510 cm−1)/polysaccharide (1060 cm−1) ratio.
Biomolecules 12 01017 g003
Table
Table 1. The 25 most abundant plant cell wall degrading CAZymes produced by O. rivulosa during growth on solid spruce wood. IP values = ion profile values. Protein IDs assigned by JGI MycoCosm (https://mycocosm.jgi.doe.gov/Obbri1/Obbri1.home.html, accessed 5 April 2022). Enzyme abbreviations are presented in Supplementary Table S1a.
Table 1. The 25 most abundant plant cell wall degrading CAZymes produced by O. rivulosa during growth on solid spruce wood. IP values = ion profile values. Protein IDs assigned by JGI MycoCosm (https://mycocosm.jgi.doe.gov/Obbri1/Obbri1.home.html, accessed 5 April 2022). Enzyme abbreviations are presented in Supplementary Table S1a.
Protein Abundance (IP Values)
Time Point (Weeks)
Protein IDCAZyEnzymeSubstrate/
Function		248
790443AA3_3AOXH2O2-supply408132144
641261CBM1-GH5_7MANgalacto(gluco)
mannan		2146870
838746CBM1-GH10XLNxylan1662614
724015CE16HAEdiverse1105032
731121GH7-CBM1CBHIcellulose832911
891614AA2MnP *lignin59--5
833133AA9-CBM1LPMOdiverse502227
821398AA9LPMOdiverse4615--
812963GH131EGLcellulose431615
851185CBM1-GH10XLNxylan43----
789567GH35LACdiverse372952
476379CBM1-GH6CBHIIcellulose376--
719765GH5_7MANgalacto(gluco)
mannan		361719
885712AA3_2GOXH2O2-supply301010
788967CBM1-GH5_5EGLcellulose281324
849432GH27AGLgalacto(gluco)
mannan		261819
753990GH2MNDgalacto(gluco)
mannan		251830
749512CBM1-CE16HAEdiverse22----
819531GH5_22BXLxylan213248
813927GH2MNDgalacto(gluco)
mannan		211429
664751AA8-AA3_1CDHcellulose2167
816606CE16HAEdiverse17----
762191CE15GExylan161312
784936GH3BGLcellulose151313
627233GH3BGLcellulose151022
* Hakala et al. (2006) [36].
Table
Table 2. The 25 most abundant plant cell wall degrading CAZymes produced by G. subvermispora during growth on solid spruce wood. IP values = ion profile values. Protein IDs assigned by JGI MycoCosm (https://mycocosm.jgi.doe.gov/Cersu1/Cersu1.home.html, accessed 5 April 2022). Enzyme abbreviations are presented in Supplementary Table S1d.
Table 2. The 25 most abundant plant cell wall degrading CAZymes produced by G. subvermispora during growth on solid spruce wood. IP values = ion profile values. Protein IDs assigned by JGI MycoCosm (https://mycocosm.jgi.doe.gov/Cersu1/Cersu1.home.html, accessed 5 April 2022). Enzyme abbreviations are presented in Supplementary Table S1d.
Protein Abundance (IP Values)
Time Point (Weeks)
Protein_IDCAZyEnzymeSubstrate/
Function		248
80773AA3_3AOXH2O2-supply739163266
105539AA2MnPlignin2632421218
59733CBM1-GH10XLNxylan1766019
49863AA2MnPlignin1334012
94795CBM1-GH5_7MANgalacto(gluco)
mannan		110619
46564AA9LPMOdiverse925931
136606GH7-CBM1CBHIcellulose85685
115721GH5_22BXLxylan774036
66688AA9-CBM1LPMOdiverse73728
50686AA2MnPlignin5614616
80500GH2MNDgalacto(gluco)
mannan		462118
97858CBM1-GH10XLNxylan4316--
84557AA3_2AAOH2O2-supply423847
84792AA8-AA3_1CDHcellulose392612
162360GH51ABFdiverse34159
94398AA2MnPlignin264--
116159AA3_2AAOH2O2-supply256--
118322CE15GExylan241839
147790GH5_7MANgalacto(gluco)
mannan		231514
118801AA1_1LCClignin23----
85281GH27AGLgalacto(gluco)
mannan		221316
117120GH3BGLcellulose22108
117436AA2MnPlignin212336
67561CBM1-GH10XLNxylan205--
79557CBM1-GH5_5EGLcellulose18166
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Marinovíc, M.; Di Falco, M.; Aguilar Pontes, M.V.; Gorzsás, A.; Tsang, A.; de Vries, R.P.; Mäkelä, M.R.; Hildén, K. Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora. Biomolecules 2022, 12, 1017. https://doi.org/10.3390/biom12081017

AMA Style

Marinovíc M, Di Falco M, Aguilar Pontes MV, Gorzsás A, Tsang A, de Vries RP, Mäkelä MR, Hildén K. Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora. Biomolecules. 2022; 12(8):1017. https://doi.org/10.3390/biom12081017

Chicago/Turabian Style

Marinovíc, Mila, Marcos Di Falco, Maria Victoria Aguilar Pontes, András Gorzsás, Adrian Tsang, Ronald P. de Vries, Miia R. Mäkelä, and Kristiina Hildén. 2022. "Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora" Biomolecules 12, no. 8: 1017. https://doi.org/10.3390/biom12081017

APA Style

Marinovíc, M., Di Falco, M., Aguilar Pontes, M. V., Gorzsás, A., Tsang, A., de Vries, R. P., Mäkelä, M. R., & Hildén, K. (2022). Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora. Biomolecules, 12(8), 1017. https://doi.org/10.3390/biom12081017

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