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Article

Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants

by
Janusz Malarz
1,
Agnieszka Galanty
2 and
Anna Stojakowska
1,*
1
Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna Street 12, 31-343 Kraków, Poland
2
Department of Pharmacognosy, Jagiellonian University Medical College, Medyczna Street 9, 30-688 Kraków, Poland
*
Author to whom correspondence should be addressed.
Plants 2022, 11(21), 2815; https://doi.org/10.3390/plants11212815
Submission received: 20 September 2022 / Revised: 16 October 2022 / Accepted: 20 October 2022 / Published: 23 October 2022
(This article belongs to the Special Issue Plant Extracts and Their Cytotoxic Activities)
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Abstract

:
Carpesium divaricatum Sieb. & Zucc. is a plant species rich in terpenoids of anti-inflammatory and cytotoxic activity, especially germacranolides of potential medicinal value. The present study describes in vitro multiplication of C. divaricatum, analysis of active constituents in the multiple shoots, and assessment of cytotoxic activities of extracts prepared from in vitro- and field-grown plants. The plant extracts were evaluated for cytotoxicity using two melanoma cell lines (HTB140 and A375); human keratinocytes (HaCaT); two colon cancer cell lines (Caco2 and HT29); human hepatocellular carcinoma cells (HepG2); two lines of prostate cancer cells (DU145 and PC3) and prostate epithelial cells (PNT2). Chemical compositions of the assayed extracts were analyzed by HPLC/DAD, in reference to isolated compounds. Maximum of 4.07 ± 1.61 shoots regenerated from a nodal explant of C. divaricatum, cultivated in a liquid MS medium supplemented with thidiazuron (1 μM). In vitro grown shoots and plantlets of C. divaricatum accumulated terpenoids that are known as active constituents of the intact plant. Cytotoxic activity of the extracts prepared from the in vitro cultured plants was like that demonstrated by the extracts prepared from field-grown plants and seemed to be more selective than cytotoxicities of the individual germacranolides.

1. Introduction

Except for the widespread C. cernuum L. and C. abrotanoides L., the genus Carpesium L. (Asteraceae, Inuleae-Inulinae) comprises ca. 20 Asian species, including six endemics in China [1,2]. Plants of Carpesium spp. are rich in sesquiterpene lactones and monoterpenoid thymol derivatives of well documented anti-inflammatory and cytotoxic activity [3,4]. Germacranolides isolated from the plants were frequently studied in respect of their cytotoxic activity against human cancer cells in vitro [5,6,7,8], but only a few studies dealt with the molecular mechanism of action of the compounds [9,10,11]. Recently, a paper reporting on poor selectivity of the cytotoxic effect exerted by Carpesium germacranolides has been published [12]. This may raise a question about the safety of internal use of the plant and its preparations. Despite a long-lasting traditional use of C. divaricatum Sieb. & Zucc. plants as both medicines and food, correlations between the chemical composition of the plant extracts and their biological activity are poorly investigated.
Though plant tissue culture offers a good experimental system for studying biosynthesis of biologically active plant metabolites and mechanisms of its regulation, there are no data on the production of biologically active terpenoids by in vitro cultures of Carpesium. The only study on in vitro culture of Carpesium spp. [13] described an effect of light quality on growth and selected physiological parameters (photosynthetic pigments, antioxidant enzymes, radical scavenging activity) of C. triste Maxim. plants cultivated in vitro and did not contain any data on biologically active mono- and sesquiterpenoids. Multiple shoots of Telekia speciosa (Schreb.) Baumg. (Asteraceae, Inuleae-Inulinae), a species closely taxonomically related to C. divaricatum [14,15] produced both thymol derivatives and sesquiterpene lactones characteristic of the parent plant [16,17].
The present study was aimed at assessing productivity of in vitro cultured shoots and/or plantlets of C. divaricatum with particular interest in sesquiterpene lactones and thymol derivatives of known biological activity. Cytotoxic activities of extracts from in vitro cultured plant material and from the leaves and roots of the field-grown plants were investigated to compare the effects exerted by the extracts on different cancer and normal cell lines.

2. Results

2.1. In Vitro Culture

Shoot culture of C. divaricatum was initiated from nodal explants excised from aseptic seedlings. Two liquid and three solidified nutrient media of different composition were used for in vitro proliferation of shoots. Two illumination regimes were also applied (see Table 1). After 8 weeks of culture, the number of shoots regenerated from each nodal explant and dry weight (DW) of shoots/plantlets developed from each explant was assessed. Up to the 7th week of culture, the multiple shoots obtained from nodal explants did not develop roots. At the end of the 8-week culture period, spontaneous rooting occurred at a low frequency. The maximum number of shoots (4.07 ± 1.61), as well as the maximum accumulation of the biomass (ca. 0.27 g DW), were achieved in liquid MS medium [18], supplemented with 1 µM TDZ (thidiazuron). The culture was aerated by shaking (100 r.p.m.) and maintained at 28 ± 3 °C, in a photoperiod of 16/8 (light/dark). Comparable results were obtained with the liquid medium containing a diminished concentration of TDZ.
Cultures grown on the media solidified with agar grew slower (up to 0.13 g DW after 8 weeks). Moreover, though significant differences in the number of shoots regenerated from one explant were not observed, the cultures on solidified media tended to produce fewer shoots. There was no visible difference in the leaf color of shoots grown in different illumination regimes.

2.2. Phytochemical Analysis

From the shoots grown on solidified MS medium supplemented with 0.5 µM TDZ five sesquiterpene lactones (see Figure 1), known constituents of C. divaricatum, were purified: 4β,8α-dihydroxy-5β,9β-diangeloyloxy-3-oxo-germacran-6α,12-olide (cardivarolide F), 4β,8α-dihydroxy-5β-isobutyryloxy-9β-angeloyloxy-3-oxo-germacran-6α,12-olide (cardivarolide H), 4β,8α-dihydroxy-5β-isobutyryloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide, 2α,5-epoxy-5,10-dihydroxy-6α-isobutyryloxy-9β-(3-methylbutyryloxy)-germacran-8α,12-olide, and 2α,5-epoxy-5,10-dihydroxy-6α-angeloyloxy-9β-isobutyryloxy-germacran-8α,12-olide (isocardivarolide C). The compounds were identified based on their 1H NMR spectra in a reference to previously published data [5,8,19,20,21,22]. Moreover, 1H NMR spectrum of one from the isolated subfractions allowed for identification of 4β,8α-dihydroxy-5β-angeloyloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide (cardivarolide G) [22] as another secondary metabolite produced by the shoots (Figure 1).
Chromatographic analysis of the extracts from in vitro cultured plants revealed the presence of 10-isobutyryloxy-8,9-epoxythymyl isobutyrate, the monoterpenoid formerly found in the root cultures of Inula spp. and multiple shoots of Telekia speciosa [16,17,23]. The compound (for the structure, see Figure 1) is a known metabolite of C. divaricatum [24].
To assess the contents of the identified terpenoid metabolites in the acetone extracts prepared from the plant material under study, the RP-HPLC-DAD method previously used for quantification of sesquiterpene lactones and 10-isobutyryloxy-8,9-epoxythymyl isobutyrate in Telekia speciosa [25] was applied (Table 2, Figure 2). The gradient profile was modified (see Section 4.4.2) to improve the resolution. The results of the analysis are shown in Table 2.
In the field-grown plants of C. divaricatum, germacranolides were major terpenoid constituents of aerial parts, whereas roots accumulated mainly 10-isobutyryloxy-8,9-epoxythymyl isobutyrate (Figure 2c,e). Extracts from the leaves collected in 2020 and in 2022 differed in their composition (Figure 2c,d). Extracts from the freshly collected leaves demonstrated higher contents of the compounds C and E, whereas extracts from the leaves collected in 2020 contained compound F (absent from the leaves collected in 2022). The cause of the difference may be disputable, but the effect of the storage period on the quality of the raw material is worth further studies. The extracts from roots of C. divaricatum collected in 2020 did not contain 10-isobutyryloxy-8,9-epoxythymyl isobutyrate (data not shown), probably due to the degradation of the compound.
C. divaricatum plantlets cultivated in vitro, in a liquid medium containing 0.5 µM TDZ (Figure 2a), accumulated lower amounts of germacranolides than the leaves of field-grown plants (Figure 2c,d) and the plantlets cultivated in vitro on solidified media supplemented with BA and NAA (Figure 2b), but contained a substantial amount of 10-isobutyryloxy-8,9-epoxythymyl isobutyrate. The plantlets grown on solidified media with addition of BA and NAA demonstrated a germacranolide accumulation pattern like that in leaves of the field-grown plants (Figure 2b–d).
Chromatographically analyzed extracts: 05TDZL, 20B01NS, CdN2020, CdN2022, and CdK2022 and the extracts used for cytotoxicity assays were prepared simultaneously, from the same batch of plant material and in the same manner.

2.3. Cytotoxic Activity of Extracts from In Vitro- and Field-Grown C. divaricatum

The assayed extracts, irrespectively of their chemical composition, exerted a cytotoxic effect on the cell lines used in the experiment (see Table 3). Moreover, the observed effect was dose- and time-dependent. The most susceptible to the action of extracts were Caco2 cells (IC50: 14.62–32.32 µg/mL, after 48 h) followed by HTB140 cells (IC50: 19.87–33.89 µg/mL, after 48 h). The most resistant were human keratinocytes (HaCaT; IC50 ≥ 75.64 µg/mL, after 48 h) and hepatoma cells (HepG2; IC50 ≥ 74.93 µg/mL, after 48 h). Some differences in the cytotoxic action of the examined extracts were observed. The extract from freshly collected leaves of plants grown in the open field was active against all cell lines used in the study (IC50: 27.43–98.41 µg/mL, after 24 h and IC50: 21.92–75.64 µg/mL, after 48 h) whereas the extract from leaves harvested in 2020 showed some selectivity of action on the cells. The difference may be connected with a distinct composition of the extracts (Figure 2c,d). Leaves of the field-grown plants contained no detectable amount of 10-isobutyryloxy-8,9-epoxythymyl isobutyrate. The compound was a major terpenoid constituent of freshly collected C. divaricatum roots. Despite the various composition (Figure 2c,e), both root and leaf extract were active in the assay, although their activities towards the individual cell lines differed. The extract from plantlets cultivated on a solidified nutrient medium, supplemented with BA and NAA, was especially active against PC3 and Caco2 cells (IC50 = 14.07 µg/mL and 14.62 µg/mL, respectively, after 48 h treatment). The extract contained both sesquiterpene lactones and the monoterpenoid thymol derivative (compound G).

3. Discussion

C. divaricatum is a species native to East Asia. Plants of the species cultivated in Central Europe due to late flowering fail to produce seeds. In vitro multiplication of shoots may provide plantlets for further cultivation in the open field or may be a good starting point for developing an in vitro culture system to produce biologically active plant metabolites. The multiple shoots cultivated in vitro may be also helpful in elucidation of the mechanisms implicated in the regulation of secondary metabolism of the plant.
Literature data on micropropagation of plants from the Inuleae tribe are sparse. The only study on in vitro culture of Carpesium [13] described changes in several physiological parameters of C. triste plantlets, induced by the color of light used to illuminate the cultures. The plantlets were grown on MS nutrient medium deprived of growth regulators and the data on shoot proliferation were not given. Nodal explants of Inula royleana DC., cultivated for 6 weeks on a solidified MS medium containing 5.0 μM kinetin and 0.1 μM NAA, regenerated 5.1 ± 1.9 shoots per explant [26]. Shoot tip explants of Inula germanica L., after 4 weeks of culture on MS medium supplemented with 4.44 μM BA and 0.54 μM NAA, produced from 3.4 ± 0.7 to 4.9 ± 0.9 shoots per explant. The number of regenerated shoots varied depending on the number of subcultures [27]. Similar multiplication rate (ca. 4 shoots/explant), after 4-week culture, was achieved for T. speciosa, a species closely related to C. divaricatum, using the same medium composition [16]. By extending the culture period to 8 weeks, the multiplication rate of T. speciosa had been increased over two times (up to 10.9 ± 3.1). The nutrient medium containing 4.44 μM BA and 0.54 μM NAA was less effective when applied to C. divaricatum shoot regeneration from nodal explants (multiplication rate after 8-week culture: 3.49 ± 1.22, see Table 1). Replacement of BA and NAA by TDZ (0.5 μM) did not increase the multiplication rate, but significantly increased the dry weight of plantlets regenerated from one explant (from 0.081 ± 0.017 to 0.131 ± 0.023). Liquid media supplemented with TDZ (0.5 or 1.0 μM) were more advantageous for both multiplication of shoots (although the effect was not statistically significant) and the dry weight increment. As the achieved multiplication rates were low, further optimization of the shoot multiplication procedure was needed.
In vitro cultivated shoots of the Asteraceae plants are usually a reliable source of those sesquiterpene lactones, which are accumulated by aerial parts of intact plants [28,29,30,31]. Multiple shoots and plantlets of C. divaricatum cultivated on solidified MS media containing BA and NAA and leaves of the field-grown plants accumulated comparable amounts of germacranolides (Figure 2, Table 2). Differences in terpenoid content observed in the in vitro cultivated plantlets (Table 2) may be caused by either different media composition or by the difference in the light regime. Biosynthesis of a pharmacologically active sesquiterpene lactone, artemisinin, by Artemisia annua L. tissue cultures was proven to be regulated by light [32,33]. In plant tissue cultures, fast growth is often accompanied with limited secondary metabolite production. The fast-growing C. divaricatum shoots, cultivated in liquid medium containing TDZ, produced less sesquiterpene lactones (as % DW) than the shoots grown on a solidified medium of the same composition.
Several dozen of sesquiterpene lactones were isolated from C. divaricatum plants, and germacranolides were the most frequently found metabolites of the group. Due to the co-occurrence of many structurally related compounds in the plant extract, development of the analytical method for quantification of Carpesium terpenoids in the raw extract is difficult. For a semi-quantitative estimation of terpenoid contents in the assayed plant extracts, the analytical method formerly used for quantification of sesquiterpene lactones and 10-isobutyryloxy-8,9-epoxythymyl isobutyrate in T. speciosa extracts [25] was adapted. The peak resolution was affected by the composition of the plant material under study and, to some extent, was improved by dilution of the sample.
The cell lines used for cytotoxicity evaluation were grouped in three panels, namely prostate, skin and gastrointestinal panels. Each panel comprised two cancer cell lines, differing in their metastatic potential, accompanied by non-neoplastic cell line of similar origin. The use of a non-neoplastic cells in the experiments gives the information on the selectivity of the tested samples. In the case of the gastrointestinal panel, hepatoma cell line (HepG2) was used instead of the appropriate non-neoplastic cells. This cell line is widely used in hepatotoxicity studies, as it reveals several phenotypic characteristics and many functional properties of normal liver cells [34,35].
The most susceptible cell line among all used in the study was colon adenocarcinoma Caco2, while the other colon adenocarcinoma cells (HT29) were more resistant. IC50 values estimated for the two cell lines were two- or threefold different. What is important, the HepG2 cells were almost unaffected, what indicates the selectivity of the extracts. As far as the prostate panel is concerned, the tested extracts were generally more active towards highly metastatic PC3 prostate carcinoma, compared to less metastatic DU145 prostate carcinoma. However, only low selectivity of the extracts was noted, as in most cases, non-neoplastic PNT2 prostate epithelial cells were also strongly affected. In the case of skin panel, the tested extracts were much more selective and revealed varied cytotoxic impact to two melanoma cell lines, while normal keratinocytes were affected to a lesser extent (IC50 > 75.6 µg/mL). Interestingly, highly metastatic HTB140 cells were more susceptible to the tested extracts compared to the other melanoma cell line (A375) used in the study that had less metastatic potential.
Sesquiterpene lactones are regarded as the biologically active metabolites, responsible for cytotoxic and anti-inflammatory activity of Carpesium plants [3]. Cytotoxic activity of the germacranolides isolated from C. divaricatum towards the human cancer cells in vitro was confirmed in several assays [3,8,12,21,22,36,37]. The compounds are accumulated in aerial parts of the plant and the plant roots seem to be almost deprived of them (Figure 2c–e). The plantlets cultivated in vitro, in MS liquid medium (containing 0.5 μM TDZ), were also poor producers of sesquiterpene lactones (Figure 2a). However, as it was shown in the present study, cytotoxic activity of the plant extracts and the sesquiterpene lactone content are not closely linked. Interestingly, traditional medicinal systems used whole herb of C. divaricatum as a remedy for various ailments [3]. The results of RP-HPLC-DAD analysis (Figure 2e) showed that 10-isobutyryloxy-8,9-epoxy-thymyl isobutyrate is a major terpenoid constituent of the plant roots. The compound has been recently described as an inhibitor of aberrant proliferative signaling in melanoma cells [38] and antileishmanial agent [39] and is known as a secondary metabolite synthesized by numerous species from the Asteraceae [40].
Acetone extracts from both in vitro- and field-grown plants of C. divaricatum are complex mixtures of active constituents that demonstrate diverse profiles of activity. Detailed knowledge of biochemistry of the plant, stability of the active metabolites and activity of their degradation products, new data on molecular mechanisms of action of isolated compounds combined with a development of a dedicated analytical method may lead to the better understanding of traditional use of C. divaricatum as a medicine and to potential future applications.

4. Materials and Methods

4.1. General Experimental Procedures

1NMR spectra were recorded in MeOD on a Bruker AVANCE III HD 400 (resonance frequency 400.17 MHz) spectrometer (Bruker Corp., Billerica, MA, USA). RP-HPLC separations were performed using an Agilent 1200 Series HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA) equipped with a diode array detector. Analytical chromatographic separations were conducted on a Kinetex XB-C18 column (4.6 × 250 mm, 5 μm total particle size; Phenomenex Inc., Torrance, CA, USA). Semipreparative RP-HPLC was conducted on a Vertex Plus Eurospher II 100-5 C18 column (8 × 250 mm; Knauer GmbH, Berlin, Germany), with an isocratic elution, using methanol–water (MeOH-H2O) mixtures of different polarity. Conventional column chromatography (CC) was conducted on Silica gel 60 (0.063–0.2 mm, Merck KGaA, Darmstadt, Germany). Thin-layer chromatography (TLC) separations were performed using precoated plates (Silica gel 60 without fluorescence indicator, Art. No 5553, Merck, Darmstadt, Germany). Solvents of analytical grade were purchased from Avantor Performance Materials S.A. (Gliwice, Poland). Water was purified by a Mili-Q system (Milipore Corp., Bedford, MA, USA). MeOH and MeCN of HPLC grade were bought from Merck (Darmstadt, Germany).

4.2. Plant Material

4.2.1. Field-Grown Plants

C. divaricatum plants were grown in the Garden of Medicinal Plants, Institute of Pharmacology, Polish Academy of Sciences, Kraków from seeds that were provided by the Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation (Tsukuba, Japan). Aerial parts of the plants were collected in the beginning of flowering period (August/September) and dried under shade at room temperature. A voucher specimen (3/15) was deposited in the collection kept at the Garden of Medicinal Plants, Institute of Pharmacology, Kraków, Poland.

4.2.2. In Vitro Culture

Seeds of C. divaricatum were surface sterilized and aseptically germinated on a half-strength MS (Murashige and Skoog) medium [18], solidified with 0.8% agar. Nodal explants (ca. 0.08 g fresh weight, FW, each), excised from aseptic seedlings, were inoculated onto the three variants of solidified MS medium containing different combinations of plant growth regulators: (1) 0.5 μM TDZ (thidiazuron); (2) 2.0 μM BA (6-benzyladenine) and 0.1 μM NAA (1-naphtaleneacetic acid) or (3) 4.44 μM BA and 0.54 NAA. Alternatively, the nodal explants were inoculated in liquid MS medium supplemented with either 0.5 μM or 1 μM of TDZ. All nutrient media used in the experiments contained 3% sucrose and their pH was adjusted to 5.8, before autoclaving. The explants were kept at 28 °C, those on solidified media were maintained under continuous illumination (40 μmol m−2 s−1, cool white fluorescent tubes) and those in liquid media were cultivated under the 16/8 (light/dark) photoperiod (20 μmol m−2 s−1, cool white fluorescent tubes) with shaking (100 r.p.m.), to induce multiplication of shoots. After 12 weeks of culture, secondary explants (i.e., nodal explants excised from the regenerated shoots) were transferred to the fresh culture medium of the same composition as the induction medium, for further growth. Temperature and illumination conditions remained unaltered. The regenerated shoots were subcultured every eight weeks by inoculating nodal explants (ca. 0.1 g fresh weight) into the fresh nutrient medium. The biomass, containing regenerated shoots and plantlets, was collected at the end of the growth cycle and dried at room temperature prior to the phytochemical examination.

4.3. Extraction and Isolation of Sesquiterpene Lactones

Shoots and plantlets, collected from the culture on solidified MS medium containing 0.5 μM TDZ, were dried and powdered. The dry plant material (25.7 g) was extracted with CHCl3 (5 × 0.3 L). The organic extracts were combined and concentrated in vacuo to provide 1.07 g of an oily residue. The residue was fractionated by conventional column chromatography (CC) over silica gel (80 g) using an n-hexane–EtOAc gradient solvent system (up to 100% EtOAc). Collected fractions (50 mL each) were combined as shown by TLC.
Fractions 144–147 and 148–151, eluted with n-hexane–EtOAc 7:3 (v/v), were subjected to semipreparative RP-HPLC on a Vertex Plus Eurospher II 100-5 C18 column (eluent: MeOH–H2O mixture, 3:2, v/v, isocratic mode, flow rate: 2 mL/min) to yield cardivarolide F (23.8 mg; tR—68 min) and a mixture containing cardivarolide G (20.9 mg; tR—85 min). Fractions 148–151 gave additionally cardivarolide H (3 mg; tR—54 min).
Fractions 152–154 (eluted with n-hexane-EtOAc; 7:3, v/v), after purification by semipreparative RP-HPLC (as described above), provided 4β,8α-dihydroxy-5β-isobutyryloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide (1.2 mg, tR—28 min). Fractions 166–172 and 173–179, eluted with n-hexane-EtOAc 3:2 (v/v), were submitted to chromatographic separation by RP-HPLC (eluent: MeOH-H2O mixture, 3:2, v/v, isocratic mode, flow rate: 1.5 mL/min) and yielded 2α,5-epoxy-5,10-dihydroxy-6α-isobutyryloxy-9β-(3-methylbutyryloxy)-germacran-8α,12-olide (1 mg; tR—53 min) and isocardivarolide C (3.2 mg; tR—40 min), respectively.
Isolated compounds were identified based on their 1H NMR spectra and comparison of the experimentally obtained data with those found in the literature [5,8,19,20,21,22].

4.4. RP-HPLC-DAD Analysis of Terpenoids in Plant Extracts

4.4.1. Sample Preparation

The dry and pulverized plant material (0.1 g) was extracted twice with 10 mL of acetone at room temperature for 3 h on a rotary shaker (100 r.p.m.). The extracts from the two consecutive extractions were combined and evaporated to dryness under reduced pressure to give a residue that was redissolved in 1 mL of MeOH and centrifuged (11,340× g, 5 min) prior to HPLC analysis.

4.4.2. Semi-Quantitative Assessment of 10-Isobutyryloxy-8,9-epoxythymyl Isobutyrate and Sesquiterpene Lactone Content

Isocardivarolide C (A); 2α,5-epoxy-5,10-dihydroxy-6α-isobutyryloxy-9β-(3-methylbutyryloxy)-germacran-8α,12-olide (B); cardivarolide H (C); 4β,8α-dihydroxy-5β-isobutyryloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide (D); cardivarolide F (E); cardivarolide G (F) and 10-isobutyryloxy-8,9-epoxythymyl isobutyrate (G) were identified in the examined plant extracts based on their retention times, UV spectra and co-chromatography with the formerly isolated C. divaricatum germacranolides [E1] and the thymol derivative isolated from T. speciosa. Their content in the investigated plant material was estimated using the analytical method described previously [25] with 2,3-dihydroaromaticin and 10-isobutyryloxy-8.9-epoxythymyl isobutyrate as external standards. Peak areas were measured at 205 nm. To improve resolution of the signals, the gradient timetable was slightly modified, and some samples had to be diluted (2× or 4×). Components of the mobile phase: water (A) and MeCN (B) remained unaltered but the linear gradient from 40% B to 75% B in 20 min, and 98% B in another 5 min was applied (stop time: 35 min, post time: 15 min). The remaining parameters of the method remained unchanged.

4.5. Cell Culture and Cytotoxicity Assessment

4.5.1. Preparation of Plant Extracts

The dry and pulverized plant material (1 g) was extracted two times (3 h) with 100 mL of acetone, at room temperature, with shaking. The combined extracts were concentrated in vacuo to remove the solvent. From the dry residues, the stock solutions in MeOH (10 mg/mL) were prepared. The working concentrations of the extracts (from 5 to 100 μg/mL) were obtained by dilution of the stock solutions with the culture media.

4.5.2. Assessment of Cytotoxic Activity

The experiment was performed on several human cancer and normal cell lines, grouped as follows: skin panel (melanoma HTB140, derived from metastatic site: lymph node, ATCC Hs 294T; malignant melanoma A375, ATCC CRL-1619; skin keratinocytes HaCaT), prostate panel (prostate carcinoma DU145, derived from metastatic site: brain, ATCC HTB-81; grade IV prostate carcinoma PC3, derived from metastatic site: bone, ATCC CRL- 1435; prostate epithelial cells PNT2, ECACC 95012613), and gastrointestinal panel (colorectal adenocarcinomas Caco-2, ATCC HTB-37 and HT-29, ATCC HTB- 38; hepatocellular carcinoma HepG2, ATCC HB-8065). The cells were cultured in humidified atmosphere in CO2 incubator, at 37 °C, on MEM low glucose (DU145), MEM high glucose (HTB140, A375, HaCaT), DMEM/F12 (HT29, PC3, PNT2, HepG2), and MEM with NEAA (Caco2), with 10% of bovine serum and antibiotics, as described previously [41]. Cells were seeded in 96-well plates for 24 h (1.5 × 104 cells/well), and then a fresh medium containing different concentrations of the tested extracts (5–100 µg/mL) was added. The incubation lasted 24 and 48 h. Cell viability was measured by MTT assay, as previously described [42]. The absorbance at 490 nm was measured using Synergy II Biotek (BioTek Instruments, Winooski, VT, USA) microplate reader. Cytotoxic activity was assessed based on cell viability expressed as percent of living cells. Results were means of three independent measurements (±SD). Doxorubicin (Ebewe Pharma GmbH., Unterach, Austria) was used as a reference cytostatic drug. The IC50 values were determined by plotting the percentage viability of the cells versus concentration and the adequate calculations made using either Excel or AAT Bioquest website program (https://www.aatbio.com/tools/ic50-calculator accessed on 4 September 2022).

4.6. Statistical Analysis

Data were analyzed using the Statistica software (v.13.3). The analysis of variance (one-way ANOVA) and post hoc Tukey test were used to show statistical significance with assumed p < 0.05.

5. Conclusions

In vitro cultivated shoots and plantlets of C. divaricatum produced terpenoids characteristic of the intact plant and the extracts from the in vitro cultured plant material demonstrated cytotoxic activity comparable to that of extracts from the field-grown plant. Taking into consideration the results of RP-HPLC-DAD analysis of the assayed extracts and results of the cytotoxicity assessment, the activity of extracts from both in vitro- and field-grown plants can not be unambiguously assigned to the presence of sesquiterpene lactones. Extracts of different origin demonstrated various specificity towards the cancer cells used in the study. The complicated correlation of the extract composition and cytotoxic activity should be a subject of further research.

Author Contributions

Conceptualization, A.S.; methodology, A.G., A.S. and J.M.; investigation, A.G., A.S. and J.M.; resources, A.G. and A.S.; data curation, A.G. and A.S.; writing—original draft preparation, A.S.; writing—review and editing, A.G., J.M. and A.S.; visualization, J.M.; supervision, A.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Data Availability Statement

The raw data from the study will be made available by the authors on request from any qualified researcher.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. WFO. Carpesium L. Published on the Internet. 2022. Available online: http://www.worldfloraonline.org/taxon/wfo-4000006771 (accessed on 13 September 2022).
  2. Flora of China @ eFloras 2008. Carpesium Linnaeus. Published on the Internet. Available online: http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=105695 (accessed on 13 September 2022).
  3. Zhang, J.-P.; Wang, G.-W.; Tian, X.-H.; Yang, Y.-X.; Liu, Q.-X.; Chen, L.-P.; Li, H.-L.; Zhang, W.-D. The genus Carpesium: A review of its ethnopharmacology, phytochemistry and pharmacology. J. Ethnopharmacol. 2015, 163, 173–191. [Google Scholar] [CrossRef] [PubMed]
  4. Shi, N.-N.; Hou, C.-C.; Liu, Y.; Li, K.-Y.; Mi, S.-D.; Tong, B.-L.; Zhang, M.-L. Chemical constituents of plants from the genus Carpesium. Heterocycl. Comm. 2022, 28, 95–123. [Google Scholar] [CrossRef]
  5. Gao, X.; Lin, C.-J.; Jia, Z.-J. Cytotoxic germacranolides and acyclic diterpenoids from the seeds of Carpesium triste. J. Nat. Prod. 2007, 70, 830–834. [Google Scholar] [CrossRef]
  6. Xu, D.-D.; Yan, Y.; Jiang, C.-X.; Liang, J.-J.; Li, H.-F.; Wu, Q.-X.; Zhu, Y. Sesquiterpenes and diterpenes with cytotoxic activities from the aerial parts of Carpesium humile. Fitoterapia 2018, 128, 50–56. [Google Scholar] [CrossRef] [PubMed]
  7. Zhu, N.-L.; Tang, C.; Xu, C.; Ke, C.-Q.; Lin, G.; Jenis, J.; Yao, S.; Liu, H.; Ye, Y. Cytotoxic germacrane-type sesquiterpene lactones from the whole plant of Carpesium lipskyi. J. Nat. Prod. 2019, 82, 919–927. [Google Scholar] [CrossRef]
  8. Zhang, T.; Zhang, Q.-B.; Fu, L.; Li, L.-Y.; Ma, L.-Y.; Si, J.-G.; Zhang, H.-W.; Wei, J.-H.; Yu, S.-S.; Zou, Z.-M. New antiproliferative germacranolides from Carpesium divaricatum. RSC Adv. 2019, 9, 11493–11502. [Google Scholar] [CrossRef]
  9. Chen, X.; Song, J.; Yuan, D.; Rao, Q.; Jiang, K.; Feng, S.; Zhu, G.; Yan, C.; Li, Y.; Zhu, J. Incaspitolide A extracted from Carpesium cernuum induces apoptosis in vitro via the PI3K/AKT pathway in benign prostatic hyperplasia. Biosci. Rep. 2021, 41, BSR20210477. [Google Scholar] [CrossRef]
  10. Huang, Y.; Mao, J.; Zhang, L.; Guo, H.; Yan, C.; Chen, M. Antiprostate cancer activity of ineupatolide isolated from Carpesium cernuum L. Biomed Res. Int. 2021, 5515961. [Google Scholar] [CrossRef]
  11. Huang, Y.; Mao, J.; Zhang, L.; Guo, H.; Yan, C.; Chen, M. Incaspitolide A isolated from Carpesium cernuum L. inhibits the growth of prostate cancer cells and induces apoptosis via regulation of the PI3K/Akt/xIAP pathway. Oncol. Lett. 2021, 21, 477. [Google Scholar] [CrossRef]
  12. Kłeczek, N.; Malarz, J.; Gierlikowska, B.; Skalniak, Ł.; Galanty, A.; Kiss, A.K.; Stojakowska, A. Germacranolides from Carpesium divaricatum: Some New Data on Cytotoxic and Anti-Inflammatory Activity. Molecules 2021, 26, 4644. [Google Scholar] [CrossRef]
  13. Zhao, J.; Thi, L.T.; Park, Y.G.; Jeong, B.R. Light quality affects growth and physiology of Carpesium triste Maxim. cultured in vitro. Agriculture 2020, 10, 258. [Google Scholar] [CrossRef]
  14. Anderberg, A.A.; Eldenäs, P.; Bayer, R.J.; Englund, M. Evolutionary relationships in the Asteraceae tribe Inuleae (incl. Plucheeae) evidenced by DNA sequences of ndhF; with notes on the systematic positions of some aberrant genera. Org. Divers. Evol. 2005, 5, 135–146. [Google Scholar] [CrossRef] [Green Version]
  15. Englund, M.; Pornpongrungrueng, P.; Gustafsson, M.H.G.; Anderberg, A.A. Phylogenetic relationships and generic delimitation in Inuleae subtribe Inulinae (Asteraceae) based on ITS and cpDNA sequence data. Cladistics 2009, 25, 319–352. [Google Scholar] [CrossRef] [PubMed]
  16. Stojakowska, A.; Malarz, J.; Kisiel, W. Terpenoids from a multiple shoot culture of Telekia speciosa. Acta Soc. Bot. Pol. 2011, 80, 253–256. [Google Scholar] [CrossRef] [Green Version]
  17. Wajs-Bonikowska, A.; Stojakowska, A.; Kalemba, D. Chemical Composition of Essential Oils from a Multiple Shoot Culture of Telekia speciosa and Different Plant Organs. Nat. Prod. Commun. 2012, 7, 625–628. [Google Scholar] [CrossRef] [Green Version]
  18. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962, 15, 473–497. [Google Scholar] [CrossRef]
  19. Kim, D.K.; Lee, K.R.; Zee, O.P. Sesquiterpene lactones from Carpesium divaricatum. Phytochemistry 1997, 46, 1245–1247. [Google Scholar] [CrossRef]
  20. Goswami, A.C.; Baruah, R.N.; Sharma, R.P.; Baruah, J.N.; Kulanthaivel, P.; Herz, W. Germacranolides from Inula cappa. Phytochemistry 1984, 23, 367–372. [Google Scholar] [CrossRef]
  21. Zhang, T.; Si, J.-G.; Zhang, Q.-B.; Chen, J.-H.; Ding, G.; Zhang, H.-W.; Jia, H.-M.; Zou, Z.-M. Three new highly oxygenated germacranolides from Carpesium divaricatum and their cytotoxic activity. Molecules 2018, 23, 1078. [Google Scholar] [CrossRef] [Green Version]
  22. Zhang, T.; Chen, J.-H.; Si, J.-G.; Ding, G.; Zhang, Q.-B.; Zhang, H.-W.; Jia, H.-M.; Zou, Z.-M. Isolation, structure elucidation, and absolute configuration of germecrane isomers from Carpesium divaricatum. Sci. Rep. 2018, 8, 12418. [Google Scholar] [CrossRef]
  23. Stojakowska, A.; Michalska, K.; Malarz, J. Simultaneous quantification of eudesmanolides and thymol derivatives from tissues of Inula helenium and I. royleana by reversed-phase high-performance liquid chromatography. Phytochem. Anal. 2006, 17, 157–161. [Google Scholar] [CrossRef] [PubMed]
  24. Zee, O.P.; Kim, D.K.; Lee, K.R. Thymol derivatives from Carpesium divaricatum. Arch. Pharm. Res. 1998, 21, 618–620. [Google Scholar] [CrossRef] [PubMed]
  25. Stojakowska, A.; Malarz, J.; Kisiel, W. Quantitative analysis of sesquiterpene lactones and thymol derivatives in extracts from Telekia speciosa. Phytochem. Lett. 2015, 11, 378–383. [Google Scholar] [CrossRef]
  26. Stojakowska, A.; Malarz, J. In vitro propagation of Inula royleana DC. Acta Soc. Bot. Pol. 2004, 73, 5–8. [Google Scholar] [CrossRef] [Green Version]
  27. Trejgell, A.; Kamińska, M.; Lisowska, K.; Tretyn, A. Micropropagation of Inula germanica L. from the seedlings explants. Not. Bot. Horti Agrobot. Cluj Napoca 2018, 46, 52–57. [Google Scholar] [CrossRef] [Green Version]
  28. Woerdenbag, H.J.; Lüers, J.F.J.; van Uden, W.; Pras, N.; Malingré, T.M.; Alfermann, A.W. Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L. Plant Cell Tissue Organ Cult. 1993, 32, 247–257. [Google Scholar] [CrossRef]
  29. Stojakowska, A.; Kisiel, W. Production of parthenolide in organ cultures of feverfew. Plant Cell Tissue Organ Cult. 1997, 47, 159–162. [Google Scholar] [CrossRef]
  30. Brown, A.M.G.; Lowe, K.C.; Davey, M.R.; Power, J.B. Feverfew (Tanacetum parthenium L.): Tissue culture and parthenolide synthesis. Plant Sci. 1996, 116, 223–232. [Google Scholar] [CrossRef]
  31. Malarz, J.; Stojakowska, A.; Dohnal, B.; Kisiel, W. Helenalin acetate in in vitro propagated plants of Arnica montana. Planta Med. 1993, 59, 51–53. [Google Scholar] [CrossRef]
  32. Kayani, W.K.; Kiani, B.H.; Dilshad, E.; Mirza, B. Biotechnological approaches for artemisinin production in Artemisia. World J. Microbiol. Biotechnol. 2018, 34, 54. [Google Scholar] [CrossRef]
  33. Poulson, M.E.; Thai, T. Effect of high light intensity on photoinhibition, oxyradicals and artemisinin content in Artemisia annua L. Photosynthetica 2015, 53, 403–409. [Google Scholar] [CrossRef]
  34. Štampar, M.; Breznik, B.; Filipič, M.; Žegura, B. Characterization of in vitro 3D cell model developed from human hepatocellular carcinoma (HepG2) Cell Line. Cells 2020, 9, 2557. [Google Scholar] [CrossRef] [PubMed]
  35. Nikolaou, N.; Green, C.J.; Gunn, P.J.; Hodson, L.; Tomlinson, J.W. Optimizing human hepatocyte models for metabolic phenotype and function: Effects of treatment with dimethyl sulfoxide (DMSO). Physiol. Rep. 2016, 4, e12944. [Google Scholar] [CrossRef] [PubMed]
  36. Kim, D.K.; Baek, N.I.; Choi, S.U.; Lee, C.O.; Lee, K.R.; Zee, O.P. Four new cytotoxic germacranolides from Carpesium divaricatum. J. Nat. Prod. 1997, 60, 1199–1202. [Google Scholar] [CrossRef]
  37. Zhang, T.; Si, J.-G.; Zhang, Q.-B.; Ding, G.; Zou, Z.-M. New highly oxygenated germacranolides from Carpesium divaricatum and their cytotoxic activity. Sci. Rep. 2016, 6, 27237. [Google Scholar] [CrossRef]
  38. Dürr, L.; Hell, T.; Dobrzyński, M.; Mattei, A.; John, A.; Augsburger, N.; Bradanini, G.; Reinhardt, J.K.; Rossberg, F.; Drobnjakovic, M.; et al. High-content screening pipeline for natural products targeting oncogenic signaling in melanoma. J. Nat. Prod. 2022, 85, 1006–1017. [Google Scholar] [CrossRef]
  39. Fadel, H.; Sifaoui, I.; Lόpez-Arencibia, A.; Reyes-Batlle, M.; Jiménez, I.A.; Lorenzo-Morales, J.; Ghedadba, N.; Benayache, S.; Piñero, J.E.; Bazzocchi, I.L. Antioxidant and leishmanicidal evaluation of Pulicaria inuloides root extracts: A bioguided fractionation. Pathogens 2019, 8, 201. [Google Scholar] [CrossRef] [Green Version]
  40. Talavera-Alemán, A.; Rodríguez-García, G.; López, Y.; García-Gutiérrez, H.A.; Torres-Valencia, J.; Del Río, R.E.; Cerda-García-Rojas, C.M.; Joseph-Nathan, P.; Gómez-Hurtado, M.A. Systematic evaluation of thymol derivatives possessing stereogenic or prostereogenic centers. Phytochem. Rev. 2015, 15, 251–277. [Google Scholar] [CrossRef]
  41. Sołtys, A.; Galanty, A.; Zagrodzki, P.; Grabowska, K.; Malarz, J.; Podolak, I. Sorbus intermedia (EHRH.) PERS. fruits as a novel source of biologically active triterpenoids–Comparative studies of ursolic acid derivatives with cytotoxic potential. Biomed. Pharmacother. 2022, 154, 113592. [Google Scholar] [CrossRef]
  42. Grabowska, K.; Amanowicz, K.; Paśko, P.; Podolak, I.; Galanty, A. Optimization of the extraction procedure for the phenolic-rich Glechoma hederacea L. herb and evaluation of its cytotoxic and antioxidant potential. Plants 2022, 11, 2217. [Google Scholar] [CrossRef]
Plants 11 02815 g001 550
Figure 1. Chemical structures of the terpenoids identified in C. divaricatum extracts: isocardivarolide C (A); 2α,5-epoxy-5,10-dihydroxy-6α-isobutyryloxy-9β-(3-methylbutyryloxy)-germacran-8α,12-olide (B); cardivarolide H (C); 4β,8α-dihydroxy-5β-isobutyryloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide (D); cardivarolide F (E); cardivarolide G (F) and 10-isobutyryloxy-8,9-epoxythymyl isobutyrate (G).
Figure 1. Chemical structures of the terpenoids identified in C. divaricatum extracts: isocardivarolide C (A); 2α,5-epoxy-5,10-dihydroxy-6α-isobutyryloxy-9β-(3-methylbutyryloxy)-germacran-8α,12-olide (B); cardivarolide H (C); 4β,8α-dihydroxy-5β-isobutyryloxy-9β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide (D); cardivarolide F (E); cardivarolide G (F) and 10-isobutyryloxy-8,9-epoxythymyl isobutyrate (G).
Plants 11 02815 g001
Plants 11 02815 g002a 550Plants 11 02815 g002b 550
Figure 2. Chromatographic separation (RP-HPLC-DAD; λ = 205 nm) of C. divaricatum extracts (100 mg/mL): (a) plants from a liquid MS medium containing TDZ; (b) plants grown on a solidified MS medium supplemented with 2.0 μM BA and 0.1 μM NAA; (c) leaves of field-grown plants collected in 2022; (d) leaves of the plants collected in 2020; (e) roots of a field-grown plant collected in 2022.
Figure 2. Chromatographic separation (RP-HPLC-DAD; λ = 205 nm) of C. divaricatum extracts (100 mg/mL): (a) plants from a liquid MS medium containing TDZ; (b) plants grown on a solidified MS medium supplemented with 2.0 μM BA and 0.1 μM NAA; (c) leaves of field-grown plants collected in 2022; (d) leaves of the plants collected in 2020; (e) roots of a field-grown plant collected in 2022.
Plants 11 02815 g002aPlants 11 02815 g002b
Table
Table 1. Effect of the nutrient medium and light conditions on in vitro multiplication of shoots from the nodal explants of Carpesium divaricatum after 8 weeks of culture.
Table 1. Effect of the nutrient medium and light conditions on in vitro multiplication of shoots from the nodal explants of Carpesium divaricatum after 8 weeks of culture.
Culture MediumLight ConditionsRegeneration of Shoots (%)Number of Shoots per ExplantFinal Dry Weight per Explant (g) 3
MS + 0.5 μM TDZ; solidified medium 1continuous illumination; cool white light; 40 μmol m−2 s−11003.36 ± 1.50 1,a0.131 ± 0.023 b
MS + 2.0 μM BA + 0.1 μM NAA; solidified medium 1continuous illumination; cool white light; 40 μmol m−2 s−196.83.73 ± 1.43 1,a0.067 ± 0.014 a
MS + 4.44 μM BA + 0.54 μM NAA; solidified medium 1continuous illumination; cool white light; 40 μmol m−2 s−194.53.49 ± 1.22 1,a0.081 ± 0.017 a
MS + 0.5 μM TDZ; liquid medium 2photoperiod 16/8 (light/dark);
cool white light; 20 μmol m−2 s−1		1003.78 ± 1.37 2,a0.216 ± 0.042 c
MS + 1.0 μM TDZ; liquid medium 2photoperiod 16/8 (light/dark);
cool white light; 20 μmol m−2 s−1		1004.07 ± 1.61 2,a0.269 ± 0.028 c
1 Data from seven consecutive passages (n = 61), mean ± SD; 2 Data from five consecutive passages (n = 27), mean ± SD; 3 Mean of four independent measurements ± SD. Results denoted with the same letter were not statistically different (p > 0.05; one-way ANOVA).
Table
Table 2. Contents of the identified sesquiterpene lactones (A–F) and thymol derivative (G) semi-quantitatively estimated in the plant material from in vitro culture and in the organs of field-grown plants.
Table 2. Contents of the identified sesquiterpene lactones (A–F) and thymol derivative (G) semi-quantitatively estimated in the plant material from in vitro culture and in the organs of field-grown plants.
Plant ExtractContents of the Terpenoids (% DW) 1
ABCDEFG
05TDZL 20.016 ± 0.002 a0.026 ± 0.002 a0.036 ± 0.004 a0.021 ± 0.003 a0.027 ± 0.004 a0.018 ± 0.003 a0.064 ± 0.008 a
05TDZS0.034 ± 0.003 a,b0.049 ± 0.006 b0.018 ± 0.001 b0.083 ± 0.004 b0.040 ± 0.005 a0.094 ± 0.016 b0.040 ± 0.001 b
20B01NS0.051 ± 0.004 b,c0.114 ± 0.002 c0.138 ± 0.001 c0.081 ± 0.002 b0.104 ± 0.002 b0.034 ± 0.001 a0.043 ± 0.004 b
44B05NS0.077 ± 0.010 d0.039 ± 0.001 b0.023 ± 0.001 b0.139 ± 0.019 c0.079 ± 0.002 c0.214 ± 0.021 c0.028 ± 0.004 b
CdN20200.047 ± 0.001 b0.079 ± 0.006 d0.031 ± 0.001 a0.070 ± 0.002 b0.049 ± 0.001 a0.179 ± 0.007 cnd
CdN20220.060 ± 0.007 c,d0.089 ± 0.006 d0.171 ± 0.004 d0.018 ± 0.001 a0.201 ± 0.013 dndnd
CdK2022ndndndndndnd0.118 ± 0.008 c
1 Values are means of three measurements (±SD); the means denoted by the same letter did not differ significantly (p > 0.05). Statistical analysis was done with one-way ANOVA with Tukey’s post hoc test; nd—not detected. 2 05TDZL—liquid MS medium containing 0.5 μM TDZ; 05TDZS—solidified MS medium containing 0.5 μM TDZ; 20B01NS—solidified MS medium containing 2.0 μM BA and 0.1 μM NAA; 44B05NS—solidified MS medium containing 4.44 μM BA and 0.54 μM NAA; CdN2020—leaves of the field-grown plants collected in 2020; CdN2022—leaves of the field-grown plants collected in 2022; CdK2022—roots of the field-grown plants collected in 2022.
Table
Table 3. Cytotoxic activities of extracts from in vitro- and field-grown Carpesium divaricatum, after 24 and 48 h. Doxorubicin was used as a reference cytostatic; 05TDZL—plants cultivated in a liquid MS medium containing 0.5 μM TDZ; 20B01NS—plants cultivated on a solidified MS medium containing 2.0 μM BA and 0.1 μM NAA; CdN2020—leaves of the field-grown plants collected in 2020; CdN2022—leaves of the field-grown plants collected in 2022; CdK2022—roots of the field-grown plants collected in 2022.
Table 3. Cytotoxic activities of extracts from in vitro- and field-grown Carpesium divaricatum, after 24 and 48 h. Doxorubicin was used as a reference cytostatic; 05TDZL—plants cultivated in a liquid MS medium containing 0.5 μM TDZ; 20B01NS—plants cultivated on a solidified MS medium containing 2.0 μM BA and 0.1 μM NAA; CdN2020—leaves of the field-grown plants collected in 2020; CdN2022—leaves of the field-grown plants collected in 2022; CdK2022—roots of the field-grown plants collected in 2022.
Plant Extract and Time of Exposure (h)IC50 (μg/mL) 1
Prostate PanelSkin PanelGastrointestinal Panel
PNT2Du145PC3HaCaTA375HTB140HT29Caco2HepG2
05TDZL 2427.48 ± 1.12 a79.18 ± 2.96 b44.94 ± 1.08 c>10034.96 ± 1.02 a37.94 ± 1.24 b46.53 ± 1.51 a28.51 ± 0.98 b>100
05TDZL 4821.42 ± 1.24 a54.59 ± 2.06 b32.63 ± 1.41 c>10030.40 ± 0.80 a33.89 ± 1.06 b42.11 ± 1.03 a25.64 ± 0.74 b>100
20B01NS 2443.17 ± 1.77 a76.24 ± 2.79 b26.50 ± 0.77 c>10042.29 ± 1.33 a33.97 ± 1.41 b64.78 ± 1.81 a26.13 ± 1.04 b>100
20B01NS 4830.23 ± 1.79 a42.48 ± 1.33 b14.07 ± 0.76 c94.15 ± 3.38 a32.07 ± 1.11 b19.87 ± 0.72 c52.76 ± 1.92 a14.62 ± 1.41 b>100
CdN2020 2447.04 ± 1.84 a45.59 ± 1.77 a29.00 ± 1.41 b>10068.04 ± 2.59 a39.06 ± 1.12 b47.56 ± 1.00 a24.64 ± 1.57 b>100
CdN2020 4832.89 ± 1.30 a24.19 ± 0.95 b21.18 ± 1.33 b>10042.91 ± 1.50 a26.12 ± 1.20 b21.18 ± 1.33 a16.21 ± 1.42 b98.27 ± 2.95
CdN2022 2434.88 ± 1.10 a74.36 ± 1.91 b64.48 ± 1.59 c95.05 ± 3.85 a49.91 ± 1.58 b27.43 ± 1.09 c58.45 ± 1.87 a37.42 ± 2.29 b98.41 ± 3.49 c
CdN2022 4829.69 ± 1.28 a53.56 ± 1.54 b54.04 ± 1.97 b75.64 ± 3.06 a39.79 ± 2.39 b21.92 ± 1.04 c49.06 ± 1.79 a26.36 ± 2.04 b74.93 ± 2.12 c
CdK2022 2450.62 ± 1.86 a80.61 ± 3.34 b43.61 ± 1.98 c>10074.14 ± 2.27 a46.13 ± 1.29 b75.00 ± 2.52 a53.93 ± 1.97 b>100
CdK2022 4833.81 ± 1.47 a61.28 ± 1.58 b34.56 ± 2.28 a>10068.02 ± 2.32 a28.17 ± 1.80 b64.84 ± 1.64 a32.32 ± 1.47 b>100
Doxorubicin 24 1.383.18>504.680.595.711.533.441.03
1 Mean of three independent measurements ± SD. Results denoted by the same letter were not statistically different (p > 0.05; one-way ANOVA). Comparisons were made separately for each extract, treatment duration, and each panel of the cells.
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Malarz, J.; Galanty, A.; Stojakowska, A. Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants. Plants 2022, 11, 2815. https://doi.org/10.3390/plants11212815

AMA Style

Malarz J, Galanty A, Stojakowska A. Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants. Plants. 2022; 11(21):2815. https://doi.org/10.3390/plants11212815

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Malarz, Janusz, Agnieszka Galanty, and Anna Stojakowska. 2022. "Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants" Plants 11, no. 21: 2815. https://doi.org/10.3390/plants11212815

APA Style

Malarz, J., Galanty, A., & Stojakowska, A. (2022). Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum: In Vitro- versus Field-Grown Plants. Plants, 11(21), 2815. https://doi.org/10.3390/plants11212815

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