Next Article in Journal
Biochar Addition Alters C: N: P Stoichiometry in Moss Crust-Soil Continuum in Gurbantünggüt Desert
Next Article in Special Issue
Physiological and Growth Responses of Potato (Solanum Tuberosum L.) to Air Temperature and Relative Humidity under Soil Water Deficits
Previous Article in Journal
Molecular Insights into Freezing Stress in Peach Based on Multi-Omics and Biotechnology: An Overview
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 11
Issue 6
10.3390/plants11060815
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling in Arabidopsis thaliana

by
Irfan Ullah Khan
1,†,
Akhtar Ali
1,2,†,
Shah Zareen
1,
Haris Ali Khan
1,
Chae Jin Lim
1,2,
Junghoon Park
1,2,
Jose M. Pardo
3 and
Dae-Jin Yun
1,*
1
Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Korea
2
Institute of Glocal Disease Control, Konkuk University, Seoul 05029, Korea
3
Institute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas, 41092 Seville, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2022, 11(6), 815; https://doi.org/10.3390/plants11060815
Submission received: 12 December 2021 / Revised: 3 March 2022 / Accepted: 14 March 2022 / Published: 18 March 2022
(This article belongs to the Special Issue Implications of Abscisic Acid in the Drought Stress Tolerance)
Browse Figures
Versions Notes

Abstract

:
The plant hormone, abscisic acid (ABA), is not only important for promoting abiotic stress responses but also plays a versatile and crucial role in plant immunity. The pathogen infection-induced dynamic accumulation of ABA mediates the degradation of non-expresser of PR genes 1 (NPR1) through the CUL3NPR3NPR4 proteasome pathway. However, the functional significance of NPR1 degradation by other E3 ligases in response to ABA remains unclear. Here, we report that NPR1 is induced transcriptionally by ABA and that npr1-1 mutation results in ABA insensitivity during seed germination and seedling growth. Mutants lacking NPR1 downregulate the expression of ABA-responsive transcription factors ABA INSENSITIVE4 (ABI4) and ABA INSENSITIVE5 (ABI5), and that of their downstream targets EM6, RAB18, RD26, and RD29B. The npr1-1 mutation also affects the transcriptional activity of WRKY18, which activates WRKY60 in the presence of ABA. Furthermore, NPR1 directly interacts with and is degraded by HOS15, a substrate receptor for the DDB1-CUL4 ubiquitin E3 ligase complex. Collectively, our findings demonstrate that NPR1 acts as a positive regulator of ABA-responsive genes, whereas HOS15 promotes NPR1 degradation in a proteasome-dependent manner.

1. Introduction

Plants are constantly exposed to various biotic and abiotic stresses and have evolved sophisticated mechanisms to cope with harsh environmental conditions [1]. Understanding the mechanisms underlying such adaptations is critical for securing the yield of crop plants in agriculture [2]. Phytohormones such as abscisic acid (ABA), ethylene, salicylic acid (SA), and jasmonic acid are key regulators of plant responses to adverse environmental conditions. Among these, ABA is the most extensively involved in plant abiotic stress responses, including high salinity, drought, heat, and osmotic stress [3,4,5]. ABA regulates plant growth and developmental processes, including seed dormancy, seed germination, and stomatal movement. Recent studies have shown that the crosstalk of multiple signaling pathways is regulated by phytohormones either antagonistically or synergistically between biotic and abiotic interactions [5,6]. During vegetative growth, plant responses to various environmental stresses, including drought, salinity, low temperature, oxidative stress, mechanical wounding, and pathogen attack, can be divided into ABA-dependent and ABA-independent pathways [3,4,7,8,9,10,11,12]. Previous studies have shown that there are two main types of ABA-dependent pathways in plants. The first is mediated by the basic leucine zipper/ABA-responsive element (bZIP/ABRE) system [13]. These include ABA INSENSITIVE5 (ABI5)/ABF transcription factors (ABA-responsive element binding factors) that upregulate the expression of ABA-induced genes, such as RD29A and RD29B, under stress conditions [14,15,16]. ABA also prompts MYC/MYB transcription factors (MYC2 and MYB2) to induce drought responsive RD22 expression in Arabidopsis [7,17]. Several downstream components important for ABA signaling have been characterized in the recent past. For instance, ABI1 and ABI2 are group A type-2C protein phosphatases that negatively regulate SNF1-related protein kinases 2 (SnRK2s), which phosphorylate and activate downstream AREB/ABF targets [18].
The transcriptional co-activator NPR1 regulates local and systemic acquired resistance (SAR) in response to SA. In Arabidopsis thaliana, cellular activities of NPR1 are regulated by intricate mechanisms. A defense-associated increase in SA levels induces NPR1 transcriptional activation [19]. Next, SA induces cellular changes in the redox state that promote NPR1 monomerization and nuclear localization [20]. Moreover, NPR1 protein levels are constantly regulated by their proteasome-dependent turnover [21]. Furthermore, the direct binding of SA induces conformational changes in NPR1, leading to the release of the NPR1 transactivation domain from its autoinhibitory domain [22,23,24]. Finally, SA-induced NPR1 phosphorylation on Ser11 and Ser15 promotes NPR1 transcriptional coactivator activity, its recruitment to the CUL3 NPR3NPR4-based E3-ligase complex, and subsequent protein turnover [21]. In line with this, phosphorylation of NPR1 by SnRK2.8 at S589 and T373 is required for its nuclear translocation [25]. In previous studies, two NPR1 paralogs, NPR3 and NPR4, acting as SA receptors with differential NPR1 binding affinities, were identified as adapter proteins for the CUL3-E3 ligase complex, promoting NPR1 degradation at different SA concentrations. Under normal conditions, NPR1–NPR4 interaction constantly removes NPR1 protein. Therefore, a basal level of SA is required to disrupt the NPR1–NPR4 protein interaction and maintain the basal NPR1 protein level in the cell [26].
Recent studies indicate significant crosstalk between SA and ABA during abiotic stress responses [3,27]. During a pathogen attack, the phytohormone ABA positively regulates the immune response downstream of SA by closing stomata, which provide a passive pathogen entrance passage [28,29]. Notably, in most cases, ABA levels markedly increase after SA induction during the plant–pathogen interplay [30,31]. However, if a pathogen successfully invades the apoplast, ABA acts as a negative regulator of the immune response by repressing SA biosynthesis, immune signaling, and resistance protein activity [6,32,33].
NPR1 reportedly regulates the expression of pathogenesis-related (PR) genes by binding to TGA transcription factors [34,35]. Moreover, WRKY family transcription factors are transcriptionally induced during pathogen infections, and under cold, drought, and high salinity conditions [36,37]. Genes induced during biotic and abiotic stresses often contain WRKY transcription factor binding sites (W-boxes) in their promoter regions [38]. For instance, SA biosynthesis genes contain W-boxes in their promoter regions, and NPR1 itself is under the transcriptional control of WKRY genes [19,38]. Eight WRKY genes have been identified as direct NPR1 targets during biotic and abiotic stress responses [39]. Among proteins, WRKY18, WRKY40, and WRKY60 physically interact with each other through leucine-zipper motifs at their N-termini [40]. WRKY18 overexpression enhances PR expression and resistance to invading pathogens [41]. Interestingly, WRKY18 and WRKY60 are also shown to positively regulate ABA signaling [42]. However, the exact mechanism by which NPR1 affects ABA signaling is yet to be identified.
Here, we report that NPR1 positively regulates ABA signaling via WRKY18 transcriptional activation. We observed that loss-of-function npr1-1 mutant seedlings were ABA insensitive during germination and cotyledon greening. Furthermore, compared to wild-type (Col-0), ABA-responsive genes were downregulated in npr1-1 plants. More interestingly, HOS15, a substrate receptor for the DDB1-CUL4 E3 ligase complex, interacted with and regulated NPR1 stability in an ABA-dependent manner. Taken together, our findings reveal a molecular mechanism underlying the posttranslational modification of NPR1 through HOS15 and highlight the critical role of NPR1 in regulating ABA signaling.

2. Materials and Methods

2.1. Plant Materials and Growth Condition

In the present study, Arabidopsis thaliana ecotype Columbia (Col-0) was used as the wild-type (WT). Seeds of hos15-2, npr1-1 mutants, 35S::NPR1-GFP/npr1-1/hos15-2 and 3535S::NPR1-GFP/npr1-1 were reported by Shen et al. (2020) [43]. Seeds of mutant abi2-2 (SALK_015166C) were obtained from ABRC. Seeds were surface-sterilized and germinated on 1/2 MS medium supplemented with 0.25% phytagel (horizontal MS plates) or 1.2% agar (vertical MS plates) and 2% sucrose. Plants were grown at 23 °C under long-day conditions (16 h light/8 h dark photoperiod; energy fluence rate of 80–100 µmol m−2 s−1) in a controlled culture room.

2.2. Gene Expression Analysis

We used 2 µg of total RNA extracted from seedlings using the RNeasy Plant Mini Kit (Qiagen, MD, USA), treated with DNase (SIGMA Chemical Co., St. Louis, MO, USA), for the synthesis of the first-strand cDNA using the ThermoscriptTM RT-PCR System (Invitrogen, Paisley, UK). We performed PCR amplification using e-Taq DNA polymerase (Solgent, Daejeon, Korea). The primers used in the RT-PCR or real-time PCR experiments are listed in Supplementary Table S1. The conditions of real-time PCR were as follows: 95 °C for 5 min, 45 cycles of 95 °C for 10 s and 60 °C for 30 s, followed by 95 °C for 10 s, 65 °C for 5 s, and 95 °C for 5 s.

2.3. Immunoblot Analysis and Immunoprecipitation

We used 10-day-old Arabidopsis plants, either treated or untreated with ABA or MG132, for the Western blot assays. Total proteins were extracted and immunoblot analysis was carried out using α-GFP and α-HOS15, for GFP-tagged line and HOS15 detection, respectively. Each immunoblot was incubated with the appropriate primary antibody (α-HOS15 antibody, 1:5000; α-GFP antibody, 1:3000) for 2 h at room temperature or overnight at 4 °C. The membranes were developed using a peroxidase-conjugated secondary antibody for α-rabbit antibody (GE, Little Chalfont, Buckinghamshire, UK) at a dilution of 1:4000. We carried out the co-immunoprecipitation studies in the tobacco transient assay by co-infiltration of tobacco (Nicotiana benthamiana) leaves with HOS15-GFP and NPR1-HA. The protein samples were extracted three days after the infiltration. For the co-immunoprecipitation studies, we extracted the total protein samples from N. benthamiana leaves and performed the pull-down assay using α-GFP, followed by immunoblotting with α-HA. Each immunoblot was incubated with the appropriate primary antibody, α-HA or α-GFP antibody, diluted at 1:2000 or 1:2000, for 2 h at room temperature or overnight at 4 °C, respectively. The membranes were developed using α-rat IgG (Sigma, St. Louis, MO, USA) and α-rabbit (GE, Little Chalfont, Buckinghamshire, UK) peroxidase-conjugated secondary antibodies, diluted at 1:1000 and 1:2000, respectively.

2.4. Yeast Two-Hybrid Analysis

The full-length NPR1 and HOS15 coding sequences were cloned into the gateway entry vector, pDONRTM/Zeo and then sub-cloned into the destination vectors, pDEST22 or pDEST32, containing activation and binding domains, respectively. The primers used for cloning are listed in Table S1. The plasmids were transformed into the yeast strain PJ694A. Three independent transformants were tested for the interactions. Empty vectors were used as negative controls.

3. Results

3.1. NRP1-1 Mutant Shows ABA Insensitive Phenotype

Mutant npr1-1 was isolated as a non-expresser of PR gene 1 in SA signaling, lacking the expression of PR1, PR5, and BGL2 genes in response to SA, 2,6-dichloroisonicotinic acid (INA), and avirulent bacterial pathogens [44]. To determine the possible role of NPR1 in abiotic stresses, we tested if NPR1 affected ABA signaling. Seeds of wild-type (Col-0), npr1-1, and 35S::NPR1-GFP/npr1-1 plants, along with the ABA-hypersensitive abi2-2 mutant as experimental control for the ABA response, were grown in the presence of exogenous ABA for one week. The npr1-1 mutant displayed an ABA-insensitive phenotype during germination (Figure 1A). The 35S::NPR1-GFP/npr1-1 plants showed sensitivity to ABA that was intermediate between the wild-type and abi2-2 controls (Figure 1A). The emerging radicles were counted, which showed that npr1-1 germination resulted in the highest number of radicles among all tested lines (Figure 1B). As shown in Figure 1C,D, the ABA-insensitivity was maintained in the npr1-1 plants after an additional growth period of 2 weeks, while Col-0 and, in a marginal way, abi2-2, started greening. The 35S::NPR1-GFP/npr1-1 plants were still hypersensitive and showed fewer green cotyledons (Figure 1C,D). Taken together, these results suggest that NPR1 positively regulates ABA signaling.

3.2. NPR1 Positively Regulates the Expression of ABA-Responsive Genes

Since npr1-1 showed ABA insensitivity both at the early stage and during the prolonged treatment period (Figure 1), we aimed at investigating whether NPR1 could also regulate downstream ABA-responsive genes, as NPR1 has already been shown to function as a transcriptional coactivator [45]. To test our hypothesis, 7-day-old Col-0, npr1-1, and 35S::NPR1-GFP/npr1-1 seedlings were treated with 100 µM ABA, and the transcript abundance of ABI4, ABI5, EM6, RAB18, RD26, and RD29B was analyzed. The expression of all the above-mentioned genes was downregulated in the npr1-1 mutant and upregulated in the 35S::NPR1-GFP/npr1-1 plants compared to the wild-type (Col-0) control (Figure 2), except for RAB18, which was downregulated in the npr1-1 mutant but showed no difference between Col-0 and 35S::NPR1-GFP/npr1-1 plants.
To inspect the role of NPR1 more comprehensively, we directly germinated Col-0 and npr1-1 seeds on ABA-containing MS plates and allowed them to grow for 7 days. The transcript levels of ABA-responsive genes ABI5, EM6, RAB18, RD26, and RD29B were less upregulated by ABA in npr1-1 mutant than in Col-0 plants (Figure S1). These results demonstrated that the loss of NPR1 function leads to ABA signaling suppression, resulting in ABA-insensitive phenotypes.

3.3. HOS15 Interacts with NPR1 and Promotes Its Degradation

Recently, we have shown that HOS15, a WD40-repeat-containing protein, negatively regulates ABA signaling through OST1/SNRK2.6 degradation [46]. HOS15 reportedly functions as a substrate receptor for the DDB1-CUL4 E3 ligase complex [43,47,48]. Furthermore, we described that HOS15 interacts with and regulates NPR1 protein abundance, thereby negatively regulating PR1 expression [43]. To test whether HOS15 regulates NPR1 protein abundance in response to ABA, we first confirmed HOS15 and NPR1 interaction using yeast two-hybrid and co-immunoprecipitation assays (Figure 3A,B). Next, we investigated NPR1 protein levels in transgenic plants of genotype 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1/hos15-2. As shown in Figure 3C, the NPR1 protein abundance slightly increased with ABA treatment after 4 h and then returned to control levels after 8 h. In contrast, NPR1 was highly accumulated in hos15-2 knockout plants in an ABA-independent manner (Figure 3C). Next, we tested NPR1 levels by treating 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1/hos15-2 seedlings with ABA for 4 h, subsequently washed ABA, and treated them with cycloheximide (CHX) for 4 h to inhibit de novo protein synthesis and investigate NPR1 protein stability. When treated with CHX, the NPR1 abundance declined very rapidly in 35S::NPRI-GFP/npr1-1 plants (Figure 3D). However, the NPR1 protein level remained more abundant in the hos15-2 background compared to that in the 35S::NPRI-GFP/npr1-1 plants (Figure 3D), indicating that HOS15 promotes NPR1 degradation. It must be noted that the NPR1 protein levels also dropped in hos15-2 plants after 4 h of CHX, suggesting the action of other E3 ligases that regulate NPR1 level]. Ubiquitination of NPR1 by CUL3 NPR3/NPR4 E3 ligase complex has previously been reported [49]. To test whether HOS15 also regulate NPR1 level through ubiquitination, we performed ubiquitination assay. As shown in Figure S2, NPR1 was highly ubiquitinated in WT; however, we found that in the presence of ABA the ubiquitination of NPR1 was partially reduced (Figure S2). In contrast, the ubiquitination of NPR1 was partially reduced in hos15-2 plants, suggesting the involvement of HOS15 in the ubiquitination of NPR1 (Figure S2).

3.4. NPR1 Functions Downstream of HOS15 in Response to ABA

As described in Figure 1, npr1-1 plants show ABA-insensitivity, whereas hos15-2 plants reportedly exhibit an ABA-sensitive phenotype [46]. Therefore, we aimed at investigating the epistatic effects of these two mutations. We thus crossed npr1-1 plants with hos15-2 plants and germinated their seeds on ABA-containing MS media. As expected, the npr1-1 plants were insensitive, whereas the hos15-2 plants were sensitive to ABA (Figure 4A,B). Interestingly, the npr1-1/hos15-2 double mutant plants were less sensitive to ABA than the hos15-2 single mutant (Figure 4A,B). Moreover, NPR1-overexpressing plants showed ABA hypersensitivity and a lower quantity of green cotyledons than the wild-type plants (Figure 4A,B). Taken together, these results demonstrate that NPR1, which functions downstream of HOS15, positively regulates the ABA-mediated seed germination.

3.5. NPR1 Regulates the Transcription of WRKY Genes

Previous studies have shown that WRKY18 positively regulates ABA signaling by activating the transcription of WRKY60 through competition with WRKY40, a reported negative regulator of ABA signaling [42]. It has also been reported that NPR1 is directly associated with the WRKY18 gene promoter [39]. Therefore, we next investigated the transcript abundance of these WRKY genes in npr1-1 plants. As expected, upon treatment with ABA, WRKY18 expression was downregulated in npr1-1 plants compared to wild-type plants (Figure 5). WRKY60, a direct target of WRKY18, was also downregulated in npr1-1 plants (Figure 5). In contrast, we could not find any change in the transcriptional abundance of WRKY40 (Figure 5). Taken together, these results demonstrate that NPR1 is involved in the ABA signaling regulation through the positive regulation of WRKY18 transcription. However, we could not completely exclude further possible roles of NPR1 in ABA biosynthesis and/or signaling at this stage.

4. Discussion

4.1. NPR1 Positively Regulates ABA Response

The Arabidopsis npr1-1 mutant has been isolated as a carrier strain of a recessive point mutation, which abolished SAR-responsive PR gene expression [44]. Plants carrying the npr1-1 point mutation lacked the expression of SA-, INA- (synthetic SA-analog) and chimeric pathogen-responsive reporter genes. Earlier reports have also shown that the npr1-1 point mutation results in SA-, INA- and pathogen-insensitive phenotypes during SAR induction, indicating that these inducers potentially share common signaling pathways [44]. However, the role and effector mechanism of NPR1 function in other signaling pathways, such as the ABA signaling cascade, remain elusive. We observed that the npr1-1 mutant showed an ABA-insensitive phenotype during early germination and post-germination seedling growth (Figure 1). Furthermore, ABA-responsive gene expression was also downregulated in the npr1-1 mutant, while it was upregulated in the overexpressing line compared with the wild-type (Figure 2). Taken together, these results indicate that NPR1 positively regulates the ABA signaling pathway.

4.2. NPR1 Is a Target of the CUL4-DDB1-HOS15 E3 Ubiquitin Ligase Complex

Previous reports have suggested that Arabidopsis HOS15 functions as a repressor protein in abiotic stress-related gene expression regulation through chromatin modification [47]. We have recently shown that HOS15 contains a WD40-repeat domain that functions as a substrate receptor for the CUL4-DDB1 E3 ligase complex [43,47]. CUL4 itself is reportedly a negative regulator of ABA signaling, and it is involved in the proteasomal degradation of ABI5, involving ABD1, DWA1, and DWA2 (WD40 repeat proteins) (summarized by Ali et al., 2020) [50], and the cul4cs mutant line displays an ABA-sensitive phenotype [51]. As shown in Figure 3, NPR1 interacts with HOS15, a part of the CUL4-DDB1 complex, suggesting that NPR1 is a target of the CUL4-DDB1 E3 ligase complex that may be involved in NPR1 protein degradation. Ubiquitination is a common mechanism to promote target protein degradation. Plant growth and development are largely affected by the ubiquitin-mediated degradation of target protein stability, whereas the recognition and target specificity of the ubiquitination pathway is mainly controlled by the substrate recruitment of E3 ubiquitin ligases [52,53]. Previous studies have shown that NPR1 is degraded by CUL3-E3 ligase in the presence of ABA [49]. However, NPR1 degradation was not completely blocked in the absence of CUL3, raising the possibility that CUL3 might be not the only E3 ligase that promotes NPR1 degradation. Therefore, we investigated the CUL4-DDB1 complex-dependent NPR1 protein stability through HOS15, as NPR1 interacts with this complex (Figure 3A,B) [43]. The NPR1 protein level slightly increased by the 4-h ABA treatment, then quickly reduced after 8 h in WT plants (Figure 3C). In contrast, NPR1 was continuously stable in hos15-2 knockout plants, suggesting that HOS15 promotes NPR1 degradation (Figure 3C). During the cycloheximide (CHX)-mediated blocking of the protein synthesis, the NPR1 protein was degraded markedly faster in WT compared to hos15-2 (Figure 3D). These results strongly suggest that the CUL4-DDB1-HOS15 ubiquitin E3-ligase complex mediates NPR1 proteasomal degradation.

4.3. NPR1 Acts Downstream of HOS15 and Regulates WRKY Gene Expression

The cul4cs mutants reportedly display a hypersensitive phenotype to exogenously applied ABA and negatively regulate the ABA signaling pathway [51]. Interestingly, the hos15-2 loss-of-function mutant, lacking the substrate receptor for the CUL4-DDB1 E3 ligase complex, also shows an ABA-hypersensitive phenotype [47], indicating that HOS15 and CUL4 function together as negative regulators of ABA signaling. Furthermore, we recently reported that NPR1 is a target of the CUL4-DDB1-HOS15 E3 ligase complex in the context of NPR1-mediated activation of plant immunity [43]. Since npr1-1 shows ABA insensitivity, while hos15-2 shows an ABA-sensitive phenotype, we tested the phenotype of the npr1-1/hos15-2 double mutant plants (Figure 1). Interestingly, the NPR1 mutation (npr1-1) suppressed the ABA sensitivity of hos15-2 in the double mutant (Figure 4), indicating that NPR1 acts downstream of HOS15 in ABA signaling.
NPR1 can directly associate with various WRKY gene promoters [39]. Among these, WRKY18, which is the direct target of NPR1, has been shown to interact with WRKY40 through a leucine-zipper motif at their N-terminal regions [40]. WRKY18 overexpression results in the hyper-induction of the PR gene and confers resistance to invading pathogens [41]. Interestingly, WRKY18 and WRKY60 are also reportedly involved in the positive regulation of ABA signaling and the regulation of ABA-responsive genes by directly regulating ABI4 and ABI5 promoters [42]. The expression of WRKY18 was downregulated in npr1-1, whereas that of WRKY60 was not induced at all in npr1-1 in response to ABA (Figure 6). These results indicate that NPR1 activates WRKY18 expression in the very early stage of ABA stress, which in turn activates WRKY60 to regulate the ABA response.
In addition to dehydration responses, plants also close their stomatal pores as part of their innate immunological response to keep bacteria out [28,29]. The role of NPR1 as one of the major regulators of plant immunity has already been well documented [21,22,23,43,45]. The identification of NPR1 as a positive regulator of ABA signaling now highlights its involvement in ABA-dependent pathogen response, which represents a major goal for future studies.
Plants 11 00815 g006 550
Figure 6. Hypothetical model. During ABA stress, NPR1 regulates ABA-responsive stress-related gene expression and activates WRKY18, which in turn competes with WRKY40 to regulate WRKY60 and activate the downstream ABA response. However, NPR1 interacts with HOS15, a substrate receptor for the CUL4-DDB1 E3-ligase ubiquitin complex, and gradually, after the activation of the WRKY genes and ABA response, NPR1 is degraded in an ABA-dependent manner. NPR1 is also degraded by CUL3 NPR3/NPR4 E3 ligase complex in an ABA dependent manner [Modified from, [49]].
Figure 6. Hypothetical model. During ABA stress, NPR1 regulates ABA-responsive stress-related gene expression and activates WRKY18, which in turn competes with WRKY40 to regulate WRKY60 and activate the downstream ABA response. However, NPR1 interacts with HOS15, a substrate receptor for the CUL4-DDB1 E3-ligase ubiquitin complex, and gradually, after the activation of the WRKY genes and ABA response, NPR1 is degraded in an ABA-dependent manner. NPR1 is also degraded by CUL3 NPR3/NPR4 E3 ligase complex in an ABA dependent manner [Modified from, [49]].
Plants 11 00815 g006

4.4. Working Model for the Role of NPR1 in the ABA Pathway

ABA is a major phytohormone that plays a crucial role in biotic and abiotic stress responses [3,4,54]. The ABA signaling pathway is well characterized and recent research efforts have focused primarily on exploring more regulatory components of this widely studied signaling cascade. Our findings suggest that NPR1 positively regulates ABA signaling by activating the transcription of WRKY18, which regulates WRKY60 and the ABA response (Figure 6). However, NPR1 interacts with HOS15, a substrate receptor for the CUL4-DDB1 E3-ligase complex, which promotes NPR1 degradation upon ABA.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants11060815/s1, Figure S1: Expression analysis of downstream ABA-responsive genes in Col-0 and npr1-1; Figure S2: HOS15 promotes NPR1 ubiquitination. NPR1 is ubiquitinated in by HOS15; Table S1: List of qRT-PCR primers.

Author Contributions

I.U.K., A.A. and D.-J.Y. conceived and designed the research. I.U.K., A.A., S.Z., H.A.K., C.J.L. and J.P. performed the experiments. I.U.K., A.A., J.M.P. and D.-J.Y. analyzed the data and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by Konkuk University in 2021.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All the data which is presented in this paper are available in manuscript main text and Supplementary Materials.

Acknowledgments

We would like to thank all the authors, who participates throughout in this project.

Conflicts of Interest

The authors declare no competing interests.

References

  1. Atkinson, N.J.; Urwin, P.E. The interaction of plant biotic and abiotic stresses: From genes to the field. J. Exp. Bot. 2012, 63, 3523–3543. [Google Scholar] [CrossRef] [PubMed]
  2. Munns, R.; James, R.A.; Xu, B.; Athman, A.; Conn, S.J.; Jordans, C.; Byrt, C.S.; Hare, R.A.; Tyerman, S.D.; Tester, M.; et al. Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene. Nat. Biotechnol. 2012, 30, 360–364. [Google Scholar] [CrossRef] [PubMed]
  3. Cao, F.Y.; Yoshioka, K.; Desveaux, D. The roles of ABA in plant–pathogen interactions. J. Plant Res. 2011, 124, 489–499. [Google Scholar] [CrossRef] [PubMed]
  4. Finkelstein, R. Abscisic acid synthesis and response. Arab. Book 2013, 11, e0166. [Google Scholar] [CrossRef]
  5. Pozo, M.J.; López-Ráez, J.A.; Azcón, C.; García-Garrido, J.M. Phytohormones as integrators of environmental signals in the regulation of mycorrhizal symbioses. New Phytol. 2015, 205, 1431–1436. [Google Scholar] [CrossRef]
  6. Mang, H.G.; Qian, W.; Zhu, Y.; Qian, J.; Kang, H.G.; Klessig, D.F.; Hua, J. Abscisic acid deficiency antagonizes high-temperature inhibition of disease resistance through enhancing nuclear accumulation of resistance proteins SNC1 and RPS4 in Arabidopsis. Plant Cell 2012, 24, 1271–1284. [Google Scholar] [CrossRef]
  7. Shinozaki, K.; Yamaguchi-Shinozaki, K. Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Curr. Opin. Plant Biol. 2000, 3, 217–223. [Google Scholar] [CrossRef]
  8. Finkelstein, R.R.; Gampala, S.S.; Rock, C.D. Abscisic acid signaling in seeds and seedlings. Plant Cell 2002, 14 (Suppl. 1), S15–S45. [Google Scholar] [CrossRef]
  9. Xiong, L.; Schumaker, K.S.; Zhu, J.K. Cell signaling during cold, drought, and salt stress. Plant Cell 2002, 14 (Suppl. 1), S165–S183. [Google Scholar] [CrossRef]
  10. Zhu, J.K. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol. 2002, 53, 247–273. [Google Scholar] [CrossRef]
  11. Himmelbach, A.; Yang, Y.; Grill, E. Relay and control of abscisic acid signaling. Curr. Opin. Plant Biol. 2003, 6, 470–479. [Google Scholar] [CrossRef]
  12. Adie, B.A.; Pérez-Pérez, J.; Pérez-Pérez, M.M.; Godoy, M.; Sánchez-Serrano, J.J.; Schmelz, E.A.; Solano, R. ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis. Plant Cell. 2007, 19, 1665–1681. [Google Scholar] [CrossRef] [PubMed]
  13. Uno, Y.; Furihata, T.; Abe, H.; Yoshida, R.; Shinozaki, K.; Yamaguchi-Shinozaki, K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc. Natl. Acad. Sci. USA 2000, 97, 11632–11637. [Google Scholar] [CrossRef] [PubMed]
  14. Furihata, T.; Maruyama, K.; Fujita, Y.; Umezawa, T.; Yoshida, R.; Shinozaki, K.; Yamaguchi-Shinozaki, K. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc. Natl. Acad. Sci. USA 2006, 103, 1988–1993. [Google Scholar] [CrossRef]
  15. Fujii, H.; Chinnusamy, V.; Rodrigues, A.; Rubio, S.; Antoni, R.; Park, S.Y.; Cutler, S.R.; Sheen, J.; Rodriguez, P.L.; Zhu, J.K. In vitro reconstitution of an abscisic acid signalling pathway. Nature 2009, 462, 660–664. [Google Scholar] [CrossRef]
  16. Yoshida, T.; Fujita, Y.; Sayama, H.; Kidokoro, S.; Maruyama, K.; Mizoi, J.; Shinozaki, K.; Yamaguchi-Shinozaki, K. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J. 2010, 61, 672–685. [Google Scholar] [CrossRef]
  17. Abe, H.; Yamaguchi-Shinozaki, K.; Urao, T.; Iwasaki, T.; Hosokawa, D.; Shinozaki, K. Role of Arabidopsis MYC and MYB homologs in drought- and abscisic-acid-regulated gene expression. Plant Cell 1997, 9, 1859–1868. [Google Scholar]
  18. Park, S.Y.; Fung, P.; Nishimura, N.; Jensen, D.R.; Fujii, H.; Zhao, Y.; Lumba, S.; Santiago, J.; Rodrigues, A.; Chow, T.-F.F.; et al. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 2009, 324, 1068–1071. [Google Scholar] [CrossRef]
  19. Yu, D.; Chen, C.; Chen, Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell 2001, 13, 1527–1539. [Google Scholar] [CrossRef]
  20. Mou, Z.; Fan, W.; Dong, X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 2003, 113, 935–944. [Google Scholar] [CrossRef]
  21. Spoel, S.H.; Mou, Z.; Tada, Y.; Spivey, N.W.; Genschik, P.; Dong, X. Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity. Cell 2009, 137, 860–872. [Google Scholar] [CrossRef] [PubMed]
  22. Rochon, A.; Boyle, P.; Wignes, T.; Fobert, P.R.; Despres, C. The coactivator function of Arabidopsis NPR1 requires the core of its BTB/ POZ domain and the oxidation of C–terminal cysteines. Plant Cell 2006, 18, 3670–3685. [Google Scholar] [CrossRef] [PubMed]
  23. Wu, Y.; Zhang, D.; Chu, J.Y.; Boyle, P.; Wang, Y.; Brindle, I.D.; De Luca, V.; Despres, C. The Arabidopsis NPR1 protein is a receptor for the plant defense hormone salicylic acid. Cell Rep. 2012, 1, 639–647. [Google Scholar] [CrossRef] [PubMed]
  24. Manohar, M.; Tian, M.; Moreau, M.; Park, S.-W.; Choi, H.W.; Fei, Z.; Friso, G.; Asif, M.; Manosalva, P.; Von Dahl, C.C.; et al. Identification of multiple salicylic acid-binding proteins using two high throughput screens. Front. Plant Sci. 2015, 5, 777. [Google Scholar] [CrossRef]
  25. Lee, H.J.; Park, Y.J.; Seo, P.J.; Kim, J.H.; Sim, H.J.; Kim, S.G.; Park, C.M. Systemic immunity requires SnRK2.8-mediated nuclear import of NPR1 in Arabidopsis. Plant Cell 2015, 27, 3425–3438. [Google Scholar] [CrossRef]
  26. Fu, Z.Q.; Yan, S.; Saleh, A.; Wang, W.; Ruble, J.; Oka, N.; Mohan, R.; Spoel, S.; Tada, Y.; Zheng, N.; et al. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Nature 2012, 486, 228–232. [Google Scholar] [CrossRef]
  27. Ton, J.; Flors, V.; Mauch-Mani, B. The multifaceted role of ABA in disease resistance. Trends Plant Sci. 2009, 14, 310–317. [Google Scholar] [CrossRef]
  28. Melotto, M.; Underwood, W.; Koczan, J.; Nomura, K.; He, S.Y. Plant stomata function in innate immunity against bacterial invasion. Cell 2006, 126, 969–980. [Google Scholar] [CrossRef] [PubMed]
  29. Zeng, W.; He, S.Y. A prominent role of the flagellin receptor FLAGELLIN-SENSING2 in mediating stomatal response to Pseudomonas syringae pv tomato DC3000 in Arabidopsis. Plant Physiol. 2010, 153, 1188–1198. [Google Scholar] [CrossRef]
  30. Fan, J.; Hill, L.; Crooks, C.; Doerner, P.; Lamb, C. Abscisic acid has a key role in modulating diverse plant–pathogen interactions. Plant Physiol. 2009, 150, 1750–1761. [Google Scholar] [CrossRef]
  31. Gruner, K.; Griebel, T.; Navarova, H.; Attaran, E.; Zeier, J. Reprogramming of plants during systemic acquired resistance. Front. Plant Sci. 2013, 4, 00252. [Google Scholar] [CrossRef] [PubMed]
  32. Yasuda, M.; Ishikawa, A.; Jikumaru, Y.; Seki, M.; Umezawa, T.; Asami, T.; Maruyama-Nakashita, A.; Kudo, T.; Shinozaki, K.; Yoshida, S.; et al. Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis. Plant Cell 2008, 20, 1678–1692. [Google Scholar] [CrossRef] [PubMed]
  33. De Torres-Zabala, M.; Bennett, M.H.; Truman, W.H.; Grant, M.R. Antagonism between salicylic and abscisic acid reflects early host–pathogen conflict and moulds plant defence responses. Plant J. 2009, 59, 375–386. [Google Scholar] [CrossRef] [PubMed]
  34. Yoda, H.; Ogawa, M.; Yamaguchi, Y.; Koizumi, N.; Kusano, T.; Sano, H. Identification of early-responsive genes associated with the hypersensitive response to tobacco mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants. Mol. Genet. Genom. 2002, 267, 154–161. [Google Scholar] [CrossRef]
  35. Dong, J.; Chen, C.; Chen, Z. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol. 2003, 51, 21–37. [Google Scholar] [CrossRef]
  36. Pnueli, L.; Hallak-Herr, E.; Rozenberg, M.; Cohen, M.; Goloubinoff, P.; Kaplan, A.; Mittler, R. Molecular and biochemical mechanisms associated with dormancy and drought tolerance in the desert legume Retama raetam. Plant J. 2002, 31, 319–330. [Google Scholar] [CrossRef]
  37. Seki, M.; Narusaka, M.; Ishida, J.; Nanjo, T.; Fujita, M.; Oono, Y.; Kamiya, A.; Nakajima, M.; Enju, A.; Sakurai, T.; et al. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 2002, 31, 279–292. [Google Scholar] [CrossRef]
  38. Wildermuth, M.C.; Dewdney, J.; Wu, G.; Ausubel, F.M. Isochorismate synthase is required to synthesize salicylic acid for plant defence. Nature 2001, 414, 562–565. [Google Scholar] [CrossRef]
  39. Wang, D.; Amornsiripanitch, N.; Dong, X. A Genomic Approach to Identify Regulatory Nodes in the Transcriptional Network of Systemic Acquired Resistance in Plants. PLoS Pathog. 2006, 2, e123. [Google Scholar] [CrossRef]
  40. Xu, X.; Chen, C.; Fan, B.; Chen, Z. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell 2006, 18, 1310–1326. [Google Scholar] [CrossRef]
  41. Chen, C.; Chen, Z. Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol. 2002, 129, 706–716. [Google Scholar] [CrossRef]
  42. Chen, H.; Lai, Z.; Shi, J.; Xiao, Y.; Chen, Z.; Xu, X. Roles of Arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol. 2010, 10, 281. [Google Scholar] [CrossRef] [PubMed]
  43. Shen, M.; Lim, C.J.; Park, J.; Kim, J.E.; Baek, D.; Nam, J.; Lee, S.Y.; Pardo, J.M.; Kim, W.Y.; Machey, D.; et al. HOS15 is a Transcriptional Corepressor of NPR1-mediated Gene Activation of Plant Immunity. Proc. Natl. Acad. Sci. USA 2020, 117, 30805–30815. [Google Scholar] [CrossRef] [PubMed]
  44. Cao, H.; Bowling, S.A.; Gordon, A.S.; Dong, X. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell 1994, 6, 1583–1592. [Google Scholar] [CrossRef] [PubMed]
  45. Pape, S.; Thurow, C.; Gatz, C. The Arabidopsis PR-1 Promoter contains multiple integration sites for the coactivator NPR1 and SNI1. Plant Physiol. 2010, 154, 1805–1818. [Google Scholar] [CrossRef] [PubMed]
  46. Ali, A.; Kim, J.K.; Jan, M.; Khan, H.A.; Khan, I.U.; Shen, M.; Park, J.; Lim, C.J.; Hussain, S.; Baek, D.; et al. Rheostatic Control of ABA Signaling through HOS15-Mediated OST1 Degradation. Mol. Plant 2019, 12, 1447–1462. [Google Scholar] [CrossRef]
  47. Park, J.; Lim, C.J.; Shen, M.; Park, H.J.; Cha, J.Y.; Iniesto, E.; Rubio, V.; Mengistee, T.; Zhu, J.K.; Bressan, R.A.; et al. Epigenetic switch from repressive to permissive chromatin in response to cold stress. Proc. Natl. Acad. Sci. USA 2018, 115, E5400–E5409. [Google Scholar] [CrossRef]
  48. Lee, J.H.; Terzaghi, W.; Gusmaroli, G.; Charron, J.F.; Yoon, H.; Chen, H.; He, Y.J.; Xiong, Y.; Deng, X.W. Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases. Plant Cell 2008, 20, 152–167. [Google Scholar] [CrossRef]
  49. Ding, Y.; Dommel, M.; Mou, Z. Abscisic acid promotes proteasome mediated degradation of the transcription coactivator NPR1 in Arabidopsis thaliana. Plant J. 2016, 86, 20–34. [Google Scholar] [CrossRef]
  50. Ali, A.; Pardo, J.M.; Yun, D.J. Desensitization of ABA-signaling: The swing from activation to degradation. Front. Plant Sci. 2020, 11, 379. [Google Scholar] [CrossRef]
  51. Lee, J.H.; Yoon, H.J.; Terzanghi, W.; Martinez, C.; Dai, M.; Li, J.; Byun, M.O.; Deng, X.W. DWA1 and DWA2, two Arabidopsis DWD proteins components of CUL4-based E3 ligases, acts as a negative regulator in ABA signal transduction. Plant Cell 2010, 22, 1716–1732. [Google Scholar] [CrossRef]
  52. Sharma, B.; Joshi, D.; Yadav, P.K.; Gupta, A.K.; Bhatt, T.K. Role of Ubiquitin-Mediated Degradation System in Plant Biology. Front. Plant Sci. 2016, 7, 806. [Google Scholar] [CrossRef] [PubMed]
  53. Shu, K.; Yang, W. E3 ubiquitin ligases: Ubiquitous actors in plant development and abiotic stress responses. Plant Cell Physiol. 2017, 58, 1461–1476. [Google Scholar] [CrossRef] [PubMed]
  54. Berens, M.L.; Wolinska, K.W.; Spaepen, S.; Ziegler, J.; Nobori, T.; Nair, A.; Krüler, V.; Winkelmüller, T.M.; Wang, Y.; Mine, A.; et al. Balancing trade-offs between biotic and abiotic stress responses through leaf age-dependent variation in stress hormone cross-talk. Proc. Natl. Acad. Sci. USA 2019, 116, 2364–2373. [Google Scholar] [CrossRef] [PubMed]
Plants 11 00815 g001 550
Figure 1. npr1-1 mutant exhibits an ABA-insensitive phenotype. (A) npr1-1 plants show ABA insensitivity. Seeds of Col-0, abi2-2, npr1-1, and 35S::NPR1-GFP/npr1-1 grown on ½ MS media with indicated concentration of ABA. Photographs were taken 5 days after germination. (B) Radicle emergence of indicated genotypes grown on ½ MS media with ABA. Error bars represent SE. Significant difference was determined by a student’s t-test with a p-value < 0.05 (*). (C) ABA insensitivity of npr1-1 plants becomes more evident as the treatment time was extended to 2 weeks. Seeds of Col-0, abi2-2, npr1-1, and 35S::NPR1-GFP/npr1-1 were germinated on 1/2 MS medium supplemented with 0, 0.75 μM and 1 μM ABA. Photographs were acquired 2 weeks after germination. (D) The number of green cotyledons from each line was counted after 10 days of treatment with 0.75 μM and 1 μM ABA. The error bars represent the standard error (SE; n = 3, independent experiments performed in triplicate). Significant difference was determined by a student’s t-test with a p-value of <0.05 (*) or <0.01 (**).
Figure 1. npr1-1 mutant exhibits an ABA-insensitive phenotype. (A) npr1-1 plants show ABA insensitivity. Seeds of Col-0, abi2-2, npr1-1, and 35S::NPR1-GFP/npr1-1 grown on ½ MS media with indicated concentration of ABA. Photographs were taken 5 days after germination. (B) Radicle emergence of indicated genotypes grown on ½ MS media with ABA. Error bars represent SE. Significant difference was determined by a student’s t-test with a p-value < 0.05 (*). (C) ABA insensitivity of npr1-1 plants becomes more evident as the treatment time was extended to 2 weeks. Seeds of Col-0, abi2-2, npr1-1, and 35S::NPR1-GFP/npr1-1 were germinated on 1/2 MS medium supplemented with 0, 0.75 μM and 1 μM ABA. Photographs were acquired 2 weeks after germination. (D) The number of green cotyledons from each line was counted after 10 days of treatment with 0.75 μM and 1 μM ABA. The error bars represent the standard error (SE; n = 3, independent experiments performed in triplicate). Significant difference was determined by a student’s t-test with a p-value of <0.05 (*) or <0.01 (**).
Plants 11 00815 g001
Plants 11 00815 g002 550
Figure 2. Expression analysis of downstream ABA-responsive genes in npr1-1 and 35S:NPR1-GFP/npr1-1. Relative mRNA level of ABA-responsive genes in the presence and absence of ABA in Col-0, npr1-1, and 35S::NPR1-GFP/npr1-1 determined by qRT-PCR using total RNA extracted from 7-day-old seedlings treated without (Control) or with 100 µM ABA for 6 h in MS liquid media. Ubiquitin was used as a control for normalization. The bars represent the mean ± standard error of three biological samples with technical replicates. Significant difference was determined by a student’s t-test with a p-value of <0.05 (*) or <0.01 (**).
Figure 2. Expression analysis of downstream ABA-responsive genes in npr1-1 and 35S:NPR1-GFP/npr1-1. Relative mRNA level of ABA-responsive genes in the presence and absence of ABA in Col-0, npr1-1, and 35S::NPR1-GFP/npr1-1 determined by qRT-PCR using total RNA extracted from 7-day-old seedlings treated without (Control) or with 100 µM ABA for 6 h in MS liquid media. Ubiquitin was used as a control for normalization. The bars represent the mean ± standard error of three biological samples with technical replicates. Significant difference was determined by a student’s t-test with a p-value of <0.05 (*) or <0.01 (**).
Plants 11 00815 g002
Plants 11 00815 g003 550
Figure 3. HOS15 interacts with and regulates NPR1 protein abundance. (A) HOS15 interacts with NPR1 in yeast. The co-transformed yeast strains were plated onto the control −TL selective −TLH media. The combinations with the empty plasmids were used as negative controls. BD, pDEST32 (bait plasmid); AD, pDEST22 (prey plasmid). (B) Co-immunoprecipitation assay between HOS15 and NPR1. Protein extracts obtained from N. benthamiana leaves infiltrated with Agrobacterium tumefaciens harboring 35S::HOS15-GFP and 35S::NPR1-HA were analyzed using anti-GFP and anti-HA antibodies, respectively. The protein extracts (input) were immunoprecipitated using anti-GFP antibody. The immunoblots were analyzed using anti-GFP and anti-HA antibodies to detect the interaction between HOS15 and NPR1. (C) The NPR1 protein degradation was determined using 10-day-old seedlings of 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1 hos15-2 treated with 100 μM ABA. The samples were collected at the indicated time point, and NPR1 protein level was measured using anti-GFP antibody. (D) Ten-day-old seedlings of 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1 hos15-2 were pretreated with 100 μM ABA for 4 h and then ABA was washed out, and the seedlings were retreated with 200 μM cycloheximide (CHX) for the next 4 h. Samples were collected and analyzed with Western blotting using anti-GFP and anti-HOS15 antibodies.
Figure 3. HOS15 interacts with and regulates NPR1 protein abundance. (A) HOS15 interacts with NPR1 in yeast. The co-transformed yeast strains were plated onto the control −TL selective −TLH media. The combinations with the empty plasmids were used as negative controls. BD, pDEST32 (bait plasmid); AD, pDEST22 (prey plasmid). (B) Co-immunoprecipitation assay between HOS15 and NPR1. Protein extracts obtained from N. benthamiana leaves infiltrated with Agrobacterium tumefaciens harboring 35S::HOS15-GFP and 35S::NPR1-HA were analyzed using anti-GFP and anti-HA antibodies, respectively. The protein extracts (input) were immunoprecipitated using anti-GFP antibody. The immunoblots were analyzed using anti-GFP and anti-HA antibodies to detect the interaction between HOS15 and NPR1. (C) The NPR1 protein degradation was determined using 10-day-old seedlings of 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1 hos15-2 treated with 100 μM ABA. The samples were collected at the indicated time point, and NPR1 protein level was measured using anti-GFP antibody. (D) Ten-day-old seedlings of 35S::NPRI-GFP/npr1-1 and 35S::NPRI-GFP/npr1-1 hos15-2 were pretreated with 100 μM ABA for 4 h and then ABA was washed out, and the seedlings were retreated with 200 μM cycloheximide (CHX) for the next 4 h. Samples were collected and analyzed with Western blotting using anti-GFP and anti-HOS15 antibodies.
Plants 11 00815 g003
Plants 11 00815 g004 550
Figure 4. npr1-1 mutation is epistatic to hos15-2. (A) npr1-1 mutation suppresses the ABA sensitive phenotype of hos15-2. Seeds of Col-0, abi2-2, hos15-2, npr1-1, npr1-1/hos15-2 double mutant, and 35S::NPR1-GFP/npr1-1 strains were germinated on 1/2 MS medium with the indicated supplement of ABA. Photographs were acquired 2 weeks after germination. (B) The green cotyledons in (A) were counted 2 weeks later, with error bars representing the SE (n = 3 independent experiments performed in triplicate). Significant difference was determined by a student’s t-test with a p-value < 0.05 (*).
Figure 4. npr1-1 mutation is epistatic to hos15-2. (A) npr1-1 mutation suppresses the ABA sensitive phenotype of hos15-2. Seeds of Col-0, abi2-2, hos15-2, npr1-1, npr1-1/hos15-2 double mutant, and 35S::NPR1-GFP/npr1-1 strains were germinated on 1/2 MS medium with the indicated supplement of ABA. Photographs were acquired 2 weeks after germination. (B) The green cotyledons in (A) were counted 2 weeks later, with error bars representing the SE (n = 3 independent experiments performed in triplicate). Significant difference was determined by a student’s t-test with a p-value < 0.05 (*).
Plants 11 00815 g004
Plants 11 00815 g005 550
Figure 5. NPR1 regulates WRKY gene expression in an ABA-dependent manner. The abundance of WRKY transcripts in npr1-1 plants under ABA stress defines their ABA-insensitive phenotype. The expression of WRKY genes in Col-0 and npr1-1 strains. Seeds of wild-type (Col-0) and npr1-1 plants were cultured on 1/2 MS medium for 2 weeks and then treated with 100 μM ABA for the indicated durations (0, 30 min, 2 h, and 4 h). Total RNA was extracted from the seedlings and analyzed using RT-qPCR. TUB8 was used as an internal control. The error bars indicate SD.
Figure 5. NPR1 regulates WRKY gene expression in an ABA-dependent manner. The abundance of WRKY transcripts in npr1-1 plants under ABA stress defines their ABA-insensitive phenotype. The expression of WRKY genes in Col-0 and npr1-1 strains. Seeds of wild-type (Col-0) and npr1-1 plants were cultured on 1/2 MS medium for 2 weeks and then treated with 100 μM ABA for the indicated durations (0, 30 min, 2 h, and 4 h). Total RNA was extracted from the seedlings and analyzed using RT-qPCR. TUB8 was used as an internal control. The error bars indicate SD.
Plants 11 00815 g005
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Khan, I.U.; Ali, A.; Zareen, S.; Khan, H.A.; Lim, C.J.; Park, J.; Pardo, J.M.; Yun, D.-J. Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling in Arabidopsis thaliana. Plants 2022, 11, 815. https://doi.org/10.3390/plants11060815

AMA Style

Khan IU, Ali A, Zareen S, Khan HA, Lim CJ, Park J, Pardo JM, Yun D-J. Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling in Arabidopsis thaliana. Plants. 2022; 11(6):815. https://doi.org/10.3390/plants11060815

Chicago/Turabian Style

Khan, Irfan Ullah, Akhtar Ali, Shah Zareen, Haris Ali Khan, Chae Jin Lim, Junghoon Park, Jose M. Pardo, and Dae-Jin Yun. 2022. "Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling in Arabidopsis thaliana" Plants 11, no. 6: 815. https://doi.org/10.3390/plants11060815

APA Style

Khan, I. U., Ali, A., Zareen, S., Khan, H. A., Lim, C. J., Park, J., Pardo, J. M., & Yun, D. -J. (2022). Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling in Arabidopsis thaliana. Plants, 11(6), 815. https://doi.org/10.3390/plants11060815

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop