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Communication

Functional Genomic Insights into Probiotic Bacillus siamensis Strain B28 from Traditional Korean Fermented Kimchi

by
Sojeong Heo
1,
Jong-Hoon Kim
2,
Mi-Sun Kwak
2,
Do-Won Jeong
1,* and
Moon-Hee Sung
2,3,*
1
Department of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Korea
2
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Korea
3
KookminBio Corporation, Seoul 02826, Korea
*
Authors to whom correspondence should be addressed.
Foods 2021, 10(8), 1906; https://doi.org/10.3390/foods10081906
Submission received: 22 June 2021 / Revised: 11 August 2021 / Accepted: 13 August 2021 / Published: 17 August 2021
(This article belongs to the Special Issue Advanced Studies of Traditional Fermented Vegetable Food Products - Kimchi and Microorganisms)
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Abstract

:
Bacillus siamensis strain B28 was previously isolated from traditional Korean fermented kimchi and inhibited expression of the microphthalmia-associated transcription factor and β-catenin in human embryonic kidney 293 cells. Here, we determined the complete genome sequence of strain B28 and compared it with other strains to elucidate its potential probiotic properties. Strain B28 does not contain antibiotic resistance-, hemolysin- or enterotoxin-encoding genes. The genome includes genes related to survival in extreme conditions, adhesion in the gut, and synthesis of the bacteriocin. Considering the potential for enhancement of human health, the strain B28 genome encodes genes related to production of eight essential amino acids, γ-aminobutyric acid, branched-chain fatty acids, γ-glutamyltransferase, and subtilisin. There are genes for the synthesis of uracil, lipoteichoic acid, glutathione, and several reactive oxygen species-scavenging enzymes. Experimentally, strain B28 exhibited sensitivity to eight antibiotics and antibacterial activity against seven foodborne pathogens. B. siamensis B28 is a safe strain with potential for development as a probiotic.

1. Introduction

The Bacillus genus comprises spore-forming bacteria found in various niches, such as oil, agricultural crops, fermented foods, and the human gastrointestinal tract [1,2,3,4,5,6]. Bacillus sp. can also be detected in kimchi using culture-dependent and -independent methods [7,8]. Bacillus spp. play important roles in the enhancement of the sensory properties of fermented foods via their amylolytic, lipolytic, and proteolytic activities [9,10,11,12,13]. Bacillus spp. have been used in the preparation of diverse fermented soybean products in northeast Asia for many centuries [14]. Furthermore, some Bacillus strains exhibit antibacterial activity against food pathogenic bacteria [15,16], anticancer effects on human colon cancer cells [17], and probiotic properties in humans and animals [18].
A B. polyfermenticus strain named B28 isolated from kimchi inhibited the expression of microphthalmia-associated transcription factor and β-catenin by human embryonic kidney 293 cells [19]. However, B. polyfermenticus has not yet been placed in the taxonomy database of the National Center for Biotechnology Information at June 2021 (NCBI; http://ncbi.nlm.nih.gov/Taxonomy) or in the List of Prokaryotic Names with Standing in Nomenclature [20]. Recently, several B. polyfermenticus strains have been reclassified: B. polyfermenticus GF423 was renamed B. velezensis variant polyfermenticus GF423 based on genome sequencing [21], and B. polyfermenticus KMU01 was reclassified as B. velezensis KMU01 [15]. Therefore, in this study, we examined the classification of strain B28 based on its whole genome sequence. We also undertook comparative genomic analysis to understand the functionality and safety of strain B28 and to assess its probiotic properties.

2. Materials and Methods

2.1. Bacterial Strains and Culture Conditions

Strain B28 was grown in tryptic soy broth (TSB; Difco, Detroit, MI, USA) to maintain its traits [15].

2.2. Genomic DNA Preparation and Genome Sequencing

Genomic DNA was prepared using a Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). Complete genome sequencing was performed using a combination of the Illumina MiSeq system (Illumina, San Diego, CA, USA) and the Single-Molecule Real-Time (SMRT) sequencing system (20 kbp; PacBio, Menlo Park, CA, USA) at ChunLab (Seoul, Korea). The generated SMRT sequencing reads (106,141 reads, 343.12-fold coverage) and MiSeq sequencing reads (6,396,282 reads, 401.906-fold depth) were assembled into three contigs by the MaSurca algorithm (version 3.3.9) [22] and plasmidSPAdes (version 3.14.1) [23]. Gene prediction was performed by Glimmer 3 and NCBI Prokaryotic Genome Annotation Pipeline (version 4.6) [24,25], and functional analysis of the annotated genes was performed using the Clusters of Orthologous Groups (COG) database [26] and SEED database (https://rast.nmpdr.org/rast.cgi accessed on June 2021).

2.3. Comparative Genomics

Genome information for B. siamensis SCSIO 05746 (GenBank accession no. GCA_002850535.1), B. siamensis KCTC 13613T (GenBank accession no. GCA_000262045.1), B. amyloliquefaciens FS1092 (GCA_004421045.1), B. amyloliquefaciens RD7-7 (GCA_001705195.1), B. velezensis JJ-D34 (GCA_000987825.1), and B. velezensis KMU01 (GCA_015277495.1) was retrieved from the NCBI database for comparative genomic analysis. The similarity of the core genome was tested via average nucleotide identity (ANI) [27]. To find orthologous genes, the EDGAR (Efficient Database framework for comparative Genome Analyses using BLASTP score Ratios) platform (EDGAR 3.0) was used [28]. Predictions of amino acid metabolic pathways were performed using the RAST (Rapid Annotation using Subsystem Technology) server [29] and iPath (Interactive Pathways Explorer; version 3.0) software [30].

2.4. Multilocus Sequence Typing

Multilocus sequence typing (MLST) developed for Bacillus was used to identify strain B28 at the species level [31]. The internal regions of eight housekeeping genes, adk, ccpA, glpF, gmk, ilvD, pur, spo0A, and tpi, were combined in the order presented; then phylogenetic trees were constructed using MEGA 7 software based on the maximum likelihood method with 1000 bootstrapping values [32].

2.5. Disc Diffusion Analysis

Antibiotic resistance was determined by the agar disk-diffusion method [33]. In brief, strain B28 was spread onto Muller-Hinton agar with eight antibiotic disks containing clindamycin (10 μg), gentamicin (10 μg), erythromycin (15 μg), lincomycin (15 μg), chloramphenicol (30 μg), tetracycline (30 μg), vancomycin (30 μg), and streptomycin (300 μg) [15]. The plate was incubated at 30 °C for 24 h before being checked for halo formation.

2.6. Hemolytic Activity Tests

β-Hemolytic activity was determined by halo formation around colonies on tryptic soy agar (Difco) supplemented with 5% (v/v) sheep blood (MBcell, Seoul, Korea). Clinically isolated Staphylococcus aureus USA300-P23 was used as a positive control [34]. The experiments were performed in triplicate.

2.7. Enterotoxin Gene Amplification

Seven enterotoxin genes were amplified from genomic DNA using specific primer sets [35]. The PCR reactions were performed using Inclone Taq polymerase (Inclone Biotech, Daejeon, Korea) according to the manufacturer’s recommended methods. The amplicons were checked on 1.2% agarose gel. Bacillus cereus KCCM 11341 was used as a positive control.

2.8. Determination of Antibacterial Activity

To check the antibacterial activity of strain B28, eight pathogenic bacteria, B. cereus KCCM 11341, Listeria monocytogenes ATCC 19111, S. aureus ATCC 12692, Alcaligenes xylosoxidans KCCM 40240, Flavobacterium sp. KCCM 11374, Escherichia coli O157:H7 EDL 933, Vibrio parahemolyticus KCTC 2729, and Salmonella enterica KCCM 11862, were used as indicator strains. Pathogens were incubated to an OD600 nm of 1.0, then spread onto TSA with a sterilized paper disk. Strain B28 was cultured in TSB to an OD600 nm of 1.0, and the supernatant was obtained after centrifugation at 5000× g for 5 min at 4 °C. After filtration of the supernatant using a 0.22-μm filter, a 15 μL aliquot was dropped onto the disk. The antibacterial activity was determined by the formation of a clear halo around the disk.

2.9. Database Accession Numbers

The complete genome sequence of B. siamensis B28 was deposited in the DDBJ/ENA/GenBank with accession numbers CP066219–CP066221. The strain was deposited in the Korean Collection for Type Cultures with accession number KCTC 13179BP.

3. Results and Discussion

3.1. Species Classification of Strain B28

Strain B28 was isolated from kimchi and identified as B. polyfermenticus in a previous investigation [19]. However, currently, the 16S rRNA gene sequence of strain B28 had 99.9% identity with those of B. amyloliquefaciens MT45 and B. siamensis SCSIO 05746 (Figure 1A). By MLST, which is used for discrimination among Bacillus species [31], B28 clustered with B. siamensis and separated from B. amyloliquefaciens (Figure 1B). In phylogenetic analysis of the eight housekeeping genes used for MLST, B28 grouped with B. siamensis (Figure S1). ANI values of the B28 genome sequence were 98.61%, 97.73%, 94.28%, and 94.06% with B. siamensis KCTC 13613T, B. siamensis SCSIO 05746, B. velezensis Y2, and B. amyloliquefaciens DSM7T, respectively. Thus, strain B28 was reclassified as Bacillus siamensis.

3.2. General Genome Characteristics of Strain B28

The complete genome of B. siamensis strain B28 included a circular chromosome (3,946,178 bp) and two circular plasmids (Table 1). The G+C mol% of the genome of strain B28 was 45.85%. The genome contained 86 tRNA genes and 27 rRNA genes. The genome of strain B28 was bigger than that of the strain type KCTC 13613T and smaller than that of strain SCSIO 05746, which was the only other complete genome of a B. siamensis strain available by 15 March 2021 (Table 1).
Analysis using COG functional categorization and SEED subsystem categorization, respectively, predicted 3573 and 1663 coding sequences (CDSs) in the genome of strain B28. COG analysis was revealed that amino acid transport and metabolism (291 genes, 8.14%) was the most abundant category, followed by transcription (266 genes, 7.44%) and carbohydrate transport and metabolism (214 genes, 5.99%) (Figure S2A). The order of abundance was similar in strain KCTC 13613T and SCSIO 05746 (Figure S2A).
In data from the SEED subsystem, 297 genes in the strain B28 genome (17.86%) were assigned to amino acid biosynthesis (Figure S2B). The next most abundant subsystem category was protein metabolism (211 genes, 12.69%), followed by carbohydrates (191 genes, 11.49%). These patterns were similar in the genome of strain KCTC 13613T and SCSIO 05746.
Strain B28 possessed the two circular plasmids pB2801 (6.1 kb) and pB2802 (5.4 kb). Two replication protein genes (B28_04055 in p2801 and B28_04064 in pB2802), which are related to plasmid replication, were detected. However, most of the genes were annotated as hypothetical protein genes.
To identify the unique genes of strain B28, we analyzed the shared genes between the genomes of B28 and SCSIO 05746, but not KCTC 13613T as the genome is incomplete. The number of genes shared by the genomes of B. siamensis strains B28 and SCSIO 05746 is illustrated in a Venn diagram (Figure S3). The two strains share 3487 CDSs in their core genome, corresponding to approximately 91.2% and 84.0% of the CDSs in strains B28 and SCSIO 05746, respectively. The majority of strain-specific genes are associated with hypothetical proteins (Table S1). However, unique assigned CDSs of strain B28 included a relaxase, transposase, α-galactosidase, and triacylglycerol lipase. The α-galactosidase (EC 3.2.1.22; gene locus: JD965_RS14670) and triacylglycerol lipase (EC 3.1.1.3; JD965_RS01465) genes were located on the chromosome. α-Galactosidase is an exoglycosidase, which breaks terminal α-1,6 galactosidase bonds of melibiose, raffinose and polymeric galactomannans [36]. This enzyme contributes to the mitigation of intestinal discomfort in humans by improving the digestibility of carbohydrates such as melibiose and stachyose, which are contained in soybean foods [37]. Triacylglycerol lipase catalyzes the hydrolysis of triacylglycerol and generates free fatty acids, monoacylglycerol, diacylglycerol, and glycerol. This enzyme contributes to the enhancement of flavor during food fermentation [38]. Therefore, these genes in strain B28 may contribute to the enhancement of human health and the sensory properties of foods if the bacterium were used as a probiotic or fermentation starter, respectively.

3.3. Insights into Virulence of B. siamensis Strain B28

The food pathogen B. cereus produces several enterotoxins: three hemolytic enterotoxins, (NblA, NblC, and NblD), three nonhemolytic enterotoxins (NheA, NheB, and NheC), and one enterotoxin T (BcET) [35,39]. Although B. siamensis is a member of the same genus as notorious pathogenic B. cereus, B. siamensis is nonpathogenic. The absence of enterotoxin-encoding genes in B. siamensis strain B28 was verified by PCR (Figure S4). Moreover, no toxin-related genes were identified in the strain B28 genome.
We also checked the antibiotic susceptibility of, and hemolysis by, strain B28. The strain was sensitive to chloramphenicol, clindamycin, erythromycin, gentamycin, lincomycin, streptomycin, tetracycline, and vancomycin, and did not exhibit β-hemolytic activity (Figure S4).
The genome of strain B28 does encode a hemolysin-III like protein gene (hlyIII; JD965_RS10690). A homologous gene was also identified in B. siamensis strain SCSIO 05746, and in the closely related species B. amyloliquefaciens and B. velezensis (Table 2). Although hemolysis was verified for HlyIII from Vibrio vulnificus on overexpression in E. coli [40], its homolog in Bacteriodes fragilis was not linked to hemolytic activity [41]. Moreover, in our previous studies, the hlyIII gene was detected regardless of phenotypic hemolysis [42,43,44,45]. Therefore, we suggest that the hlyIII gene in B. siamensis strain B28 is not related to hemolysis.
Putative efflux pumps or transporter genes for bicyclomycin, lincomycin, multiple drugs, and tetracycline were identified on the chromosomes of B. siamensis strains B28 and SCSIO 05746 (Table 2). However, strain B28 did not exhibit resistance to lincomycin or tetracycline (Figure S4). These genes have previously been identified in the genomes of several strains that did not show resistance to the respective antibiotics [15,42], and the genes were detected on the chromosomes of B. amyloliquefaciens and B. velezensis, not on plasmids. These results imply that these were not acquired antibiotic resistance genes (Table 2). Therefore, we suggest that the annotated chromosomally encoded antibiotic resistance genes in the B28 genome are not strain-specific and may not actually contribute to antibiotic resistance.
Overall, phenotypic and genomic analyses suggest that B. siamensis strain B28 is a safe bacterium for use in food and medicine.

3.4. Probiotic Properties of B. siamensis Strain B28

The live microorganisms in probiotics have health benefits for humans and animals [46]. Generally, the requirements for probiotics are acid tolerance for passage through the gastric tract (to reach the intestine); the ability to survive in harsh conditions, including in manufacturing processes and the human/animal gut; adherence in the gut; antimicrobial activity against pathogenic bacteria; carbohydrate, lipid, and protein use; and health enhancement, such as enhancement of immunogenicity.

3.4.1. Survivability of Strain B28

The genome of B. siamensis strain B28 suggests that spore-forming ability, biofilm formation, and cholylglycine hydrolase (EC 3.5.1.24; a bile salt hydrolase) might aid survival of this strain in extreme conditions (Table S2). The B28 genome encodes spore-forming genes including spore wall synthetic proteins, as well as proteins for spore germination. Species that can form spores can withstand the harsh conditions used by the food processing industry, such as high temperatures. Biofilm-producing bacteria can also persist in extreme environments, and strain B28 possesses genes related to biofilm formation. These results indicate that B. siamensis strain B28 might reach the intestinal tract via biofilm formation, spore formation, and/or the deconjugation of bile salts.

3.4.2. Adhesive Ability of Strain B28

Successful probiotics should be able to adhere in the gut [47]. Biofilms, exopolysaccharide (EPS), fibronectin, and flagella are factors related to adhesion [48,49,50,51]. The B28 genome suggests that EPS can be produced during biofilm formation (Table S2). It also contains genes related to fibronectin biosynthesis and flagellum formation.

3.4.3. Antibacterial Activity

The antibacterial activity of B. siamensis strain B28 against eight foodborne pathogens was evaluated via an agar plate diffusion method. The strain inhibited the growth of the Gram-positive strains B. cereus and S. aureus and the Gram-negative strains Alcaligenes xylosoxidans, E. coli, Flavobacterium, Salmonella enterica, and Vibrio parahaemolyticus (Figure 2).
The B28 genome includes two bacteriocin-related operons—the bacitracin operon and the mesentericin operon (Figure 2). Bacitracin is a branched cyclic dodecylpeptide antibiotic synthesized nonribosomaly [52] that interferes with cell wall synthesis [53]. The bacitracin operon of strain B28 includes genes encoding a small membrane protein (liaI), phage shock protein (liaH), antibacterial peptide (liaG), transporter (liaF), sensor kinase (liaS), and response regulator (liaR) (Figure 2). Mesentericin is a small non-lantibiotic bacteriocin that shows bactericidal activity [54]. These gene sets might confer the phenotypical antibacterial activities of strain B28. Bacteriocin-producing bacteria have an advantage in the gut. These results suggest that strain B28 is a potential probiotic with antimicrobial activity.

3.4.4. Potential for Health Enhancement by Strain B28

The strain B28 genome indicates the ability to supply the essential amino acids, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine, in the intestine via synthesis from glucose (Table S3 and Figure 3). Strain B28 also possesses genes for the production of γ-aminobutyric acid (GABA) from glutamate and branched chain fatty acids (BCFAs) from isoleucine, leucine, and valine (Figure 3). GABA and BCFAs are bioactive compounds that are important for human health; for example, they show anti-proliferative activity [55,56]. Genomic analysis also revealed that strain B28 encodes two gamma-glutamyltransferase (GGT) genes (EC 2.3.2.2; JD965_RS09945 and JD965_RS17695) involved in the production of γ-glutamyl peptide from free amino acids; γ-glutamyl peptide has beneficial effects in human tissue, such as antioxidation and anticancer activities [57]. The B. siamensis B28 genome possesses a subtilisin-encoding gene (EC 3.4.21.62; nattokinase; JD965_RS05730); subtilisin shows antithrombotic and antihypertensive effects [58]. These analyses suggest that strain B28 might supply essential amino acids and bioactive compounds to a human host through the gut.
Previously, B. siamensis strain B28 was found to inhibit the expression of microphthalmia-associated transcription factor and β-catenin in human embryonic kidney 293 cells. β-catenin is important for carcinogenesis and tumor progression. We assumed that the effect on microphthalmia-associated transcription factor contributed to the whitening effects of strain B28 [19]. To find the genetic basis for these effects, we searched the strain B28 genome for tyrosinase inhibitors (for the whitening effect) and regulators of β-catenin expression. Tyrosinase plays a key role in melanogenesis in mammals, and bacteria produce tyrosinase inhibitors during fermentation [59]. The B28 genome possesses genes for uracil, lipoteichoic acid, and glutathione synthesis; these compounds are tyrosinase inhibitors found in microorganisms [59,60] (Figure 4 and Table S4). Moreover, glutathione is involved in the scavenging of reactive oxygen species, which induces β-catenin expression [61]; the B28 genome also encodes several enzymes (catalase, peroxidase, and superoxidase dismutase) that scavenge reactive oxygen species (Table S4). Additionally, glycogen synthase kinase-3 (EC 2.7.11.1) activity could affect β-catenin expression, and the B28 genome contains eight genes for the production of glycogen synthase kinase-3 [62] (Table S4).
Bacillus siamensis was registered in 2010 [63] and the predominant species in fermented foods [64,65]. Bacillus siamensis showed the enzymatic activities which conferred the enhancement of sensory properties [64,65]. Therefore, B. siamensis was suggested as a starter candidate species for food fermentation. Our current experimental results provide genomic insights into B. siamensis strain B28 and suggest that it lacks acquired antibiotic resistance, hemolysin, and enterotoxin genes. The genome indicates that strain B28 possesses genes for survival in harsh conditions, adhesion, antibacterial activity against foodborne pathogens, essential amino acid synthesis, and the enhancement of human health. These results suggested the possibility of using B. siamensis strain B28 as a safe probiotic strain. B. siamensis strain B28 could be developed for use as a valuable food by the functional food, feed, and medical industries.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/foods10081906/s1, Figure S1: Phylogenetic trees based on the entire gene sequences of adk, ccpA, glpF, gmk, ilvD, pur, spo0A, and tpi showing the relationships between strain B28 and other Bacillus spp. The phylogenetic trees were constructed using the maximum likelihood method. Branches with bootstrap values <50% were collapsed. The distance scales are shown under the trees. Figure S2: Comparison of functional categories of genes in three Bacillus siamensis genomes based on Clusters of Orthologous Groups (COG) (A) and SEED (B) analyses. Genome sequences of B. siamensis strains B28 and SCSIO 05746 were uploaded to the COG and SEED viewer servers independently. Functional roles of annotated genes were assigned and grouped by subsystem feature categories. Colored bars indicate the number of genes assigned to each category. Figure S3: Venn diagram comparing the two B. siamensis genomes. The Venn diagram enumerates the pan-genome of strains B28 and SCSIO 05746 generated using EDGAR (the Efficient Database framework for comparative Genome Analyses using BLASTP score Ratios). The overlapping region represents common coding sequences (CDSs) shared between the B. siamensis genomes. The numbers outside the overlapping regions indicate the numbers of CDSs in each genome without homologs in the other genome. Figure S4: Test of the antibiotic susceptibility of B. siamensis strain B28 (A); hemolysis by strain B28 (B); and the presence of enterotoxin genes in strain B28 (C). Table S1: List of singletons generated by comparing the genomes of two B. siamensis strains Table S2: List of genes in strain B28 related to probiotic properties Table S3: List of genes in strain B28 related to amino acid synthesis Table S4: List of genes in strain B28 related to whitening and scavenging of reactive oxygen species.

Author Contributions

Conceptualization, D.-W.J. and M.-H.S.; methodology, S.H., J.-H.K. and M.-S.K.; data curation, S.H., J.-H.K., M.-S.K. and D.-W.J.; investigation, S.H., J.-H.K., M.-S.K. and D.-W.J.; funding acquisition, S.H., D.-W.J. and M.-H.S.; project administration, D.-W.J. and M.-H.S.; supervision: D.-W.J. and M.-H.S.; writing-original draft, S.H., D.-W.J. and M.-H.S.; and writing-review and editing: S.H., D.-W.J. and M.-H.S. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by an Innopolis Korea Foundation 2020 grant funded by the Government of Korea (MEST) (No. 2020-JB-RD-0218), the National Research Foundation of Korea (NRF) (NRF-2019R1A2C10036391), and a Korea Environmental Industry and Technology Institute (KEITI) grant funded by the Ministry of Environment of Korea.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in the article.

Acknowledgments

We thank Jochen Blom at Justus-Liebig University for the EDGAR analysis. We thank James Allen, DPhil for editing a draft of this manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Adimpong, D.B.; Sorensen, K.I.; Thorsen, L.; Stuer-Lauridsen, B.; Abdelgadir, W.S.; Nielsen, D.S.; Derkx, P.M.; Jespersen, L. Antimicrobial susceptibility of Bacillus strains isolated from primary starters for African traditional bread production and characterization of the bacitracin operon and bacitracin biosynthesis. Appl. Environ. Microbiol. 2012, 78, 7903–7914. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Ahaotu, I.; Anyogu, A.; Njoku, O.H.; Odu, N.N.; Sutherland, J.P.; Ouoba, L.I. Molecular identification and safety of Bacillus species involved in the fermentation of African oil beans (Pentaclethra macrophylla Benth) for production of Ugba. Int. J. Food Microbiol. 2013, 162, 95–104. [Google Scholar] [CrossRef] [PubMed]
  3. Eom, J.S.; Seo, B.Y.; Choi, H.S. Biogenic mine degradation by Bacillus species isolated from traditional fermented soybean food and detection of decarboxylase-related genes. J. Microbiol. Biotechnol. 2015, 25, 1519–1527. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Jeong, D.W.; Jeong, M.; Lee, J.H. Antibiotic susceptibilities and characteristics of Bacillus licheniformis isolates from traditional Korean fermented soybean foods. LWT Food Sci. Technol. 2017, 75, 565–568. [Google Scholar] [CrossRef]
  5. Lee, B.; Lee, H.; Jeong, D.W.; Lee, J.H. A rapid isolation method for Bacillus coagulans from Rice Straw. Microbiol. Biotechnol. Lett. 2015, 43, 401–404. [Google Scholar] [CrossRef]
  6. Hong, H.A.; Khaneja, R.; Tam, N.M.K.; Cazzato, A. Bacillus subtilis isolated from the human gastrointestinal tract. Res. Microbiol. 2008, 160, 134–143. [Google Scholar] [CrossRef]
  7. An, D.; Kim, H.R.; Jeong, D.W.; Caldwell, J.M.; Lee, J.H. Bacterial community monitoring of commercial kimchi produced in Korea and China with evidence of bacilli spore formation during fermentation. Microbiol. Biotechnol. Lett. 2014, 42, 121–130. [Google Scholar] [CrossRef] [Green Version]
  8. Cho, M.S.; Jin, Y.J.; Kang, B.K.; Park, Y.K.; Kim, C.; Park, D.S. Understanding the ontogeny and succession of Bacillus velezensis and B. subtilis subsp. subtilis by focusing on kimchi fermentation. Sci. Rep. 2018, 8, 7045. [Google Scholar]
  9. Jeong, D.W.; Lee, H.; Jeong, K.; Kim, C.T.; Shim, S.T.; Lee, J.H. Effects of starter candidates and NaCl on the production of volatile compounds during soybean fermentation. J. Microbiol. Biotechnol. 2019, 29, 191–199. [Google Scholar] [CrossRef] [PubMed]
  10. Lee, J.H.; Jeong, D.W.; Kim, M.K. Influence of bacterial starter cultures on the sensory characteristics of doenjang, a fermented soybean paste, and their impact on consumer hedonic perception. J. Sens. Stud. 2019, 34, e12508. [Google Scholar] [CrossRef]
  11. Sharma, R.; Garg, P.; Kumar, P.; Bhatia, S.K.; Kulshrestha, S. Microbial fermentation and its role in quality improvement of fermented foods. Fermentation 2020, 6, 106. [Google Scholar] [CrossRef]
  12. Anisha, A.H.; Anandham, R.; Kwon, S.W.; Gandhi, P.I.; Gopal, N.O. Evaluation of Bacillus spp. as dough starters for Adhirasam—A traditional rice based fermented food of Southern India. Braz. J. Microbiol. 2015, 46, 1183–1191. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Jeong, D.W.; Heo, S.; Lee, B.; Lee, H.; Jeong, K.; Her, J.Y.; Lee, K.G.; Lee, J.H. Effects of the predominant bacteria from meju and doenjang on the production of volatile compounds during soybean fermentation. Int. J. Food Microbiol. 2017, 262, 8–13. [Google Scholar] [CrossRef] [PubMed]
  14. Shin, D.; Jeong, D. Korean traditional fermented soybean products: Jang. J. Ethn. Foods 2015, 2, 2–7. [Google Scholar] [CrossRef] [Green Version]
  15. Heo, S.; Kim, J.H.; Kwak, M.S.; Sung, M.H.; Jeong, D.W. Functional annotation genome unravels potential probiotic Bacillus velezensis strain KMU01 from traditional Korean fermented Kimchi. Foods 2021, 10, 563. [Google Scholar] [CrossRef] [PubMed]
  16. Kim, Y.S.; Lee, J.; Heo, S.; Lee, J.H.; Jeong, D.W. Technology and safety evaluation of Bacillus coagulans exhibiting antimicrobial activity for starter development. LWT Food Sci. Technol. 2021, 137, 110464. [Google Scholar] [CrossRef]
  17. Ma, E.L.; Choi, Y.J.; Choi, J.; Pothoulakis, C.; Rhee, S.-J.H.; Im, E. The anticancer effect of probiotic Bacillus polyfermenticus on human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition. Int. J. Cancer 2010, 127, 780–790. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  18. Hong, H.A.; Duc, L.H.; Cutting, S.M. The use of bacterial spore formers as probiotics. FEMS Microbiol. Rev. 2005, 29, 813–835. [Google Scholar] [CrossRef] [Green Version]
  19. Oh, S.; Sung, M.H. A Cosmetic Composition Comprising Bacillus polyfermenticus Extract. Korean Patent 10-1843926, 26 March 2018. [Google Scholar]
  20. Parte, A.C.; Sarda Carbasse, J.; Meier-Kolthoff, J.P.; Reimer, L.C.; Goker, M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Int. J. Syst. Evol. Microbiol. 2020, 70, 5607–5612. [Google Scholar] [CrossRef]
  21. Jeong, H.; Kim, J.; Choi, S.K.; Pan, J.G. Genome sequence of the probiotic strain Bacillus velezensis variant polyfermenticus GF423. Microbiol. Res. Announc. 2018, 7, e01000-18. [Google Scholar] [CrossRef] [Green Version]
  22. Zimin, A.V.; Marcais, G.; Puiu, D.; Roberts, M.; Salzberg, S.L.; Yorke, J.A. The MaSuRCA genome assembler. Bioinformatics 2013, 29, 2669–2677. [Google Scholar] [CrossRef] [Green Version]
  23. Antipov, D.; Hartwick, N.; Shen, M.; Raiko, M.; Lapidus, A.; Pevzner, P.A. plasmidSPAdes: Assembling plasmids from whole genome sequencing data. Bioinformatics 2016, 32, 3380–3387. [Google Scholar] [CrossRef] [Green Version]
  24. Kelley, D.R.; Liu, B.; Delcher, A.L.; Pop, M.; Salzberg, S.L. Gene prediction with Glimmer for metagenomic sequences augmented by classification and clustering. Nucleic. Acids Res. 2012, 40, e9. [Google Scholar] [CrossRef] [PubMed]
  25. Tatusova, T.; DiCuccio, M.; Badretdin, A.; Chetvernin, V.; Nawrocki, E.P.; Zaslavsky, L.; Lomsadze, A.; Pruitt, K.D.; Borodovsky, M.; Ostell, J. NCBI prokaryotic genome annotation pipeline. Nucleic. Acids Res. 2016, 44, 6614–6624. [Google Scholar] [CrossRef] [PubMed]
  26. Tatusov, R.L.; Koonin, E.V.; Lipman, D.J. A genomic perspective on protein families. Science 1997, 278, 631–637. [Google Scholar] [CrossRef] [Green Version]
  27. Goris, J.; Konstantinidis, K.T.; Klappenbach, J.A.; Coenye, T.; Vandamme, P.; Tiedje, J.M. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int. J. Syst. Evol. Microbiol. 2007, 57, 81–91. [Google Scholar] [CrossRef] [Green Version]
  28. Blom, J.; Kreis, J.; Spanig, S.; Juhre, T.; Bertelli, C.; Ernst, C.; Goesmann, A. EDGAR 2.0: An enhanced software platform for comparative gene content analyses. Nucleic. Acids Res. 2016, 44, W22–W28. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Aziz, R.K.; Bartels, D.; Best, A.A.; DeJongh, M.; Disz, T.; Edwards, R.A.; Formsma, K.; Gerdes, S.; Glass, E.M.; Kubal, M.; et al. The RAST Server: Rapid annotations using subsystems technology. BMC Genom. 2008, 9, 75. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  30. Darzi, Y.; Letunic, I.; Bork, P.; Yamada, T. iPath3.0: Interactive pathways explorer v3. Nucleic Acids Res. 2018, 46, W510–W513. [Google Scholar] [CrossRef] [Green Version]
  31. Jeong, D.W.; Lee, B.; Lee, H.; Jeong, K.; Jang, M.; Lee, J.H. Urease characteristics and phylogenetic status of Bacillus paralicheniformis. J. Microbiol. Biotechnol. 2018, 28, 1992–1998. [Google Scholar] [CrossRef] [Green Version]
  32. Kumar, S.; Stecher, G.; Tamura, K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 2016, 33, 1870–1874. [Google Scholar] [CrossRef] [Green Version]
  33. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement; CLSI: Wayne, PA, USA, 2007. [Google Scholar]
  34. Jeong, D.W.; Cho, H.; Lee, H.; Li, C.; Garza, J.; Fried, M.; Bae, T. Identification of the P3 promoter and distinct roles of the two promoters of the SaeRS two-component system in Staphylococcus aureus. J. Bacteriol. 2011, 193, 4672–4684. [Google Scholar] [CrossRef] [Green Version]
  35. Abbas, B.A.; Khudor, M.H.; Saeed, B.M.S. Detection of hbl, nhe and bceT toxin genes in Bacillus cereus isolates by multiplex PCR. Int. J. Curr. Microbiol. App. Sci. 2014, 3, 1009–1016. [Google Scholar]
  36. Liu, X.; Champagne, C.P.; Lee, B.H.; Boye, J.I.; Casgrain, M. Thermostability of probiotics and their alpha-galactosidases and the potential for bean products. Biotechnol. Res. Int. 2014, 2014, 472723. [Google Scholar] [CrossRef]
  37. Di Stefano, M.; Miceli, E.; Gotti, S.; Missanelli, A.; Mazzocchi, S.; Corazza, G.R. The effect of oral alpha-galactosidase on intestinal gas production and gas-related symptoms. Dig. Dis. Sci. 2007, 52, 78–83. [Google Scholar] [CrossRef] [PubMed]
  38. Seitz, E.W. Microbial and enzyme-induced flavors in dairy foods. J. Dairy Sci. 1990, 73, 3664–3691. [Google Scholar] [CrossRef]
  39. Senesi, S.; Ghelardi, E. Production, secretion and biological activity of Bacillus cereus enterotoxins. Toxins 2010, 2, 1690–1703. [Google Scholar] [CrossRef] [PubMed]
  40. Chen, Y.C.; Chang, M.C.; Chuang, Y.C.; Jeang, C.L. Characterization and virulence of hemolysin III from Vibrio vulnificus. Curr. Microbiol. 2004, 49, 175–179. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  41. Robertson, K.P.; Smith, C.J.; Gough, A.M.; Rocha, E.R. Characterization of Bacteroides fragilis hemolysins and regulation and synergistic interactions of HlyA and HlyB. Infect. Immun. 2006, 74, 2304–2316. [Google Scholar] [CrossRef] [Green Version]
  42. Jeong, D.W.; Heo, S.; Ryu, S.; Blom, J.; Lee, J.H. Genomic insights into the virulence and salt tolerance of Staphylococcus equorum. Sci. Rep. 2017, 7, 5383. [Google Scholar] [CrossRef] [Green Version]
  43. Heo, S.; Lee, J.H.; Jeong, D.W. Complete genome sequence of Staphylococcus xylosus strain DMSX03 from fermented soybean, meju. Korean J. Microbiol. 2021, 57, 52–54. [Google Scholar]
  44. Jeong, D.W.; Lee, B.; Heo, S.; Jang, M.; Lee, J.H. Complete genome sequence of Bacillus licheniformis strain 0DA23-1, a potential starter culture candidate for soybean fermentation. Korean J. Microbiol. 2018, 54, 453–455. [Google Scholar]
  45. Lee, J.; Heo, S.; Kim, Y.-S.; Lee, J.H.; Jeong, D.W. Complete genome sequence of Bacillus coagulans strain ASRS217, a potential food fermentation starter culture. Korean J. Microbiol. 2020, 56, 321–323. [Google Scholar]
  46. Binda, S.; Hill, C.; Johansen, E.; Obis, D.; Pot, B.; Sanders, M.E.; Tremblay, A.; Ouwehand, A.C. Criteria to qualify microorganisms as “Probiotic” in foods and dietary supplements. Front. Microbiol. 2020, 11, 1662. [Google Scholar] [CrossRef]
  47. Granato, D.; Bergonzelli, G.E.; Pridmore, R.D.; Marvin, L.; Rouvet, M.; Corthesy-Theulaz, I.E. Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins. Infect. Immun. 2004, 72, 2160–2169. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  48. Henderson, B.; Nair, S.; Pallas, J.; Williams, M.A. Fibronectin: A multidomain host adhesin targeted by bacterial fibronectin-binding proteins. FEMS Microbiol. Rev. 2011, 35, 147–200. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Salas-Jara, M.J.; Ilabaca, A.; Vega, M.; Garcia, A. Biofilm Forming Lactobacillus: New challenges for the development of probiotics. Microorganisms 2016, 4, 35. [Google Scholar] [CrossRef] [PubMed]
  50. Haiko, J.; Westerlund-Wikstrom, B. The role of the bacterial flagellum in adhesion and virulence. Biology 2013, 2, 1242–1267. [Google Scholar] [CrossRef] [Green Version]
  51. Dons, L.; Eriksson, E.; Jin, Y.; Rottenberg, M.E.; Kristensson, K.; Larsen, C.N.; Bresciani, J.; Olsen, J.E. Role of flagellin and the two-component CheA/CheY system of Listeria monocytogenes in host cell invasion and virulence. Infect. Immun. 2004, 72, 3237–3244. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Neumuller, A.M.; Konz, D.; Marahiel, M.A. The two-component regulatory system BacRS is associated with bacitracin “self-resistance” of Bacillus licheniformis ATCC 10716. Eur. J. Biochem. 2001, 268, 3180–3189. [Google Scholar] [CrossRef]
  53. Ward, J.B. The synthesis of peptidoglycan in an autolysin-deficient mutant of Bacillus licheniformis N.C.T.C. 6346 and the effect of beta-lactam antibiotics, bacitracin and vancomycin. Biochem. J. 1974, 141, 227–241. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Fremaux, C.; Hechard, Y.; Cenatiempo, Y. Mesentericin Y105 gene clusters in Leuconostoc mesenteroides Y105. Microbiology 1995, 141, 1637–1645. [Google Scholar] [CrossRef] [Green Version]
  55. Nikmaram, N.; Dar, B.N.; Roohinejad, S.; Koubaa, M.; Barba, F.J.; Greiner, R.; Johnson, S.K. Recent advances in gamma-aminobutyric acid (GABA) properties in pulses: An overview. J. Sci. Food Agric. 2017, 97, 2681–2689. [Google Scholar] [CrossRef] [Green Version]
  56. Taormina, V.M.; Unger, A.L.; Schiksnis, M.R.; Torres-Gonzalez, M.; Kraft, J. Branched-chain fatty acids—An underexplored class of dairy-derived fatty acids. Nutrients 2020, 12, 2875. [Google Scholar] [CrossRef]
  57. Lee, N.R.; Go, T.H.; Lee, S.M.; Jeong, S.Y.; Park, G.T.; Hong, C.O.; Son, H.J. In vitro evaluation of new functional properties of poly-gamma-glutamic acid produced by Bacillus subtilis D7. Saudi. J. Biol. Sci. 2014, 21, 153–158. [Google Scholar] [CrossRef] [Green Version]
  58. Fujita, M.; Ohnishi, K.; Takaoka, S.; Ogasawara, K.; Fukuyama, R.; Nakamuta, H. Antihypertensive effects of continuous oral administration of nattokinase and its fragments in spontaneously hypertensive rats. Biol. Pharm. Bull. 2011, 34, 1696–1701. [Google Scholar] [CrossRef] [Green Version]
  59. Fernandes, M.S.; Kerkar, S. Microorganisms as a source of tyrosinase inhibitors: A review. Ann. Microbiol. 2017, 67, 343–358. [Google Scholar] [CrossRef]
  60. Sonthalia, S.; Daulatabad, D.; Sarkar, R. Glutathione as a skin whitening agent: Facts, myths, evidence and controversies. Indian J. Dermatol. Venereol. Leprol. 2016, 82, 262–272. [Google Scholar] [CrossRef] [PubMed]
  61. Liao, J.; Liu, P.P.; Hou, G.; Shao, J.; Yang, J.; Liu, K.; Lu, W.; Wen, S.; Hu, Y.; Huang, P. Regulation of stem-like cancer cells by glutamine through beta-catenin pathway mediated by redox signaling. Mol. Cancer 2017, 16, 51. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Hongisto, V.; Vainio, J.C.; Thompson, R.; Courtney, M.J.; Coffey, E.T. The Wnt pool of glycogen synthase kinase 3β is critical for trophic-deprivation-induced neuronal death. Mol. Cell Biol. 2008, 28, 1515–1527. [Google Scholar] [CrossRef] [Green Version]
  63. Sumpavapol, P.; Tongyonk, L.; Tanasupawat, S.; Chokesajjawatee, N.; Luxananil, P.; Visessanguan, W. Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand. Int. J. Syst. Evol. Microbiol. 2010, 60, 2364–2370. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Jeong, D.W.; Kim, H.R.; Jung, G.; Han, S.; Kim, C.T.; Lee, J.H. Bacterial community migration in the ripening of doenjang, a traditional Korean fermented soybean food. J. Microbiol. Biotechnol. 2014, 24, 648–660. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Zheng, L.; Li, D.; Li, Z.L.; Kang, L.N.; Jiang, Y.Y.; Liu, X.Y.; Chi, Y.P.; Li, Y.Q.; Wang, J.H. Effects of Bacillus fermentation on the protein microstructure and anti-nutritional factors of soybean meal. Lett. Appl. Microbiol. 2017, 65, 520–526. [Google Scholar] [CrossRef] [PubMed]
Foods 10 01906 g001 550
Figure 1. Phylogenetic analysis of 16S rRNA genes (A) and multilocus sequence typing (B) of strain B28 based on the maximum likelihood method with 1000 bootstrap values. Branches with bootstrap values lower than 50% have been collapsed. The scale of the diagram is the pairwise distance expressed as the percentage dissimilarity.
Figure 1. Phylogenetic analysis of 16S rRNA genes (A) and multilocus sequence typing (B) of strain B28 based on the maximum likelihood method with 1000 bootstrap values. Branches with bootstrap values lower than 50% have been collapsed. The scale of the diagram is the pairwise distance expressed as the percentage dissimilarity.
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Foods 10 01906 g002 550
Figure 2. Antibacterial activity (A) and annotated bacteriocin-related genes (B) of B. siamensis strain B28.
Figure 2. Antibacterial activity (A) and annotated bacteriocin-related genes (B) of B. siamensis strain B28.
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Foods 10 01906 g003 550
Figure 3. Predicted amino acid synthesis pathways in B. siamensis strain B28. Enzyme-encoding genes are displayed in green. Essential amino acids, non-essential amino acids, and other metabolites are green, orange, and blue-boxed, respectively. The black arrows indicate potential enzymatic reactions.
Figure 3. Predicted amino acid synthesis pathways in B. siamensis strain B28. Enzyme-encoding genes are displayed in green. Essential amino acids, non-essential amino acids, and other metabolites are green, orange, and blue-boxed, respectively. The black arrows indicate potential enzymatic reactions.
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Foods 10 01906 g004 550
Figure 4. Synthesis pathways of uracil (A) and glutathione (B), and operon for lipoteichoic acid synthesis (C) in B. siamensis strain B28. Potential enzyme-encoding genes are displayed in green letters. The black arrows indicate potential enzymatic reactions in the B28 genome.
Figure 4. Synthesis pathways of uracil (A) and glutathione (B), and operon for lipoteichoic acid synthesis (C) in B. siamensis strain B28. Potential enzyme-encoding genes are displayed in green letters. The black arrows indicate potential enzymatic reactions in the B28 genome.
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Table
Table 1. Genome features of Bacillus siamensis strains.
Table 1. Genome features of Bacillus siamensis strains.
FeatureB28SCSIO 05746KCTC 13613T
Size (bp)3,957,728 4,280,711 3,779,696
Chromosome size (bp)3,946,178 4,268,316 -
G+C content (mol%)45.8545.9946.30
No. of plasmids2 a1 b-
Open reading frames403443753839
CDSs assigned by COG c357337063451
CDSs assigned by SEED d166317241617
No. of rRNAs27273
No. of tRNAs868678
Contigs3251
Scaffolds0051
OriginKimchiSea mudSalted crab
ReferencesCompleteCompleteDraft
a Plasmids in strain B28: pB2801, 6.1 kb, and pB2802, 5.4 kb; b Plasmids in strain SCSIO 05746: pSCSIO05746, 12.4 kb; c COG results were retrieved from EZBioCloud data: https://www.ezbiocloud.net/ accessed on June 2021; d SEED results were retrieved from RAST data: https://rast.nmpdr.org/rast.cgi accessed on June 2021. CDSs, coding sequences; COG, Clusters of Orthologous Groups.
Table
Table 2. Potential virulence determinants identified in Bacillus siamensis and genomes of related species.
Table 2. Potential virulence determinants identified in Bacillus siamensis and genomes of related species.
Gene LocusProductGene aKEEG bCOGPresence of Gene in Bacillus Genomes
B. siamensisB. amyloliquefaciensB. velezensis
SCSIO 05746FS1092RD7-7JJ-D34KMU01
Hemolysis-related
JD965_RS10690Hemolysin-3 like proteinhlyIIIK11068S●●●●●
Antibiotic resistance
JD965_RS01450Lincomycin resistance protein lmrBK18926P●●●●●
JD965_RS01610Multidrug resistance protein K18935P●●●●●
JD965_RS02265Small multidrug resistance pumpsmrK03297P●●-●●
JD965_RS05435ATP-binding cassette, subfamily B, multidrug efflux pumpmdlA, smdAK18889V●●●●●
JD965_RS05440ATP-binding cassette, subfamily B, multidrug efflux pumpmdlB, smdAK18890V●●●●●
JD965_RS05865MFS transporterbltK08153G●●●●●
JD965_RS07140Paired small multidrug resistance pumpykkCK18924P●●●●●
JD965_RS07145Paired small multidrug resistance pumpykkDK18925P●●●●●
JD965_RS09245Small multidrug resistance pumpsmrK03297P●●●●●
JD965_RS09240Multidrug resistance protein K11815P●●●●●
JD965_RS09920Probable multidrug resistance protein K03327V●●●●●
JD965_RS10260Multidrug resistance protein, MATE familynorMK03327V●●●●●
JD965_RS12840Tetracycline resistance proteintetBK08168G●●-●●
JD965_RS18410Bicyclomycin resistance proteinbcr, tcaBK07552P●●●●●
a Gene (BlastKOALA); b KEGG (The Kyoto Encyclopedia of Genes and Genomes number is from the KEGG orthology database (http://www.genome.jp accessed on June 2021). - indicates that a gene was not identified in the strain.
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Heo, S.; Kim, J.-H.; Kwak, M.-S.; Jeong, D.-W.; Sung, M.-H. Functional Genomic Insights into Probiotic Bacillus siamensis Strain B28 from Traditional Korean Fermented Kimchi. Foods 2021, 10, 1906. https://doi.org/10.3390/foods10081906

AMA Style

Heo S, Kim J-H, Kwak M-S, Jeong D-W, Sung M-H. Functional Genomic Insights into Probiotic Bacillus siamensis Strain B28 from Traditional Korean Fermented Kimchi. Foods. 2021; 10(8):1906. https://doi.org/10.3390/foods10081906

Chicago/Turabian Style

Heo, Sojeong, Jong-Hoon Kim, Mi-Sun Kwak, Do-Won Jeong, and Moon-Hee Sung. 2021. "Functional Genomic Insights into Probiotic Bacillus siamensis Strain B28 from Traditional Korean Fermented Kimchi" Foods 10, no. 8: 1906. https://doi.org/10.3390/foods10081906

APA Style

Heo, S., Kim, J. -H., Kwak, M. -S., Jeong, D. -W., & Sung, M. -H. (2021). Functional Genomic Insights into Probiotic Bacillus siamensis Strain B28 from Traditional Korean Fermented Kimchi. Foods, 10(8), 1906. https://doi.org/10.3390/foods10081906

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