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Article

An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand

by
Supakorn Nundaeng
1,2,
Nakarin Suwannarach
2,3,
Savitree Limtong
4,5,
Surapong Khuna
2,3,
Jaturong Kumla
2,3,* and
Saisamorn Lumyong
2,3,5,*
1
Master of Science Program in Applied Microbiology (International Program), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
2
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
3
Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand
4
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
5
Academy of Science, The Royal Society of Thailand, Bangkok 10300, Thailand
*
Authors to whom correspondence should be addressed.
J. Fungi 2021, 7(11), 957; https://doi.org/10.3390/jof7110957
Submission received: 14 October 2021 / Revised: 7 November 2021 / Accepted: 8 November 2021 / Published: 11 November 2021
(This article belongs to the Special Issue Biodiversity, Distribution and Conservation of Plants and Fungi; Effects of Global Warming and Environmental Stress)
Browse Figures
Versions Notes

Abstract

:
Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae) are isolated from various habitats and distributed throughout the world. Prior to this study, 35 species had been validly published and accepted into this genus. Beneficially, Wickerhamomyces species have been used in a number of biotechnologically applications of environment, food, beverage industries, biofuel, medicine and agriculture. However, in some studies, Wickerhamomyces species have been identified as an opportunistic human pathogen. Through an overview of diversity, taxonomy and recently published literature, we have updated a brief review of Wickerhamomyces. Moreover, two new Wickerhamomyces species were isolated from the soil samples of Assam tea (Camellia sinensis var. assamica) that were collected from plantations in northern Thailand. Herein, we have identified these species as W. lannaensis and W. nanensis. The identification of these species was based on phenotypic (morphological, biochemical and physiological characteristics) and molecular analyses. Phylogenetic analyses of a combination of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) of ribosomal DNA genes support that W. lannaensis and W. nanensis are distinct from other species within the genus Wickerhamomyces. A full description, illustrations and a phylogenetic tree showing the position of both new species have been provided. Accordingly, a new combination species, W. myanmarensis has been proposed based on the phylogenetic results. A new key for species identification is provided.

1. Introduction

The genus Wickerhamomyces was first proposed by Kurtzman et al. [1] in 2008 with W. canadensis (basionym Hansenula canadensis) as the type species. This genus belongs to the family Wickerhamomycetaceae of the order Saccharomycetales [1]. Wickerhamomyces species can reproduce both asexually and sexually. Through asexual reproduction, the species reproduce by budding and some species produce pseudohyphae and/or true hyphae. Alternatively, in sexual reproduction they produce hat-shaped or spherical ascospores with an equatorial ledge for sexual reproduction [1,2]. Most of the known Wickerhamomyces species can utilize various carbon sources, but not methanol or hexadecane. Nitrate utilization was observed in some species, while the diazonium blue B reaction was negative for all species. The predominant ubiquinone in the Wickerhamomyces species is CoQ-7 [1,3].
Most species of the genus Wickerhamomyces have been transferred from the genera Candida, Hansenula, Pichia and Williopsis based on phylogenetic analyses [1,2,4,5,6]. Currently, a total of 35 species have been accepted and recorded in the Index Fungorum [7]. A phylogenetic study of Arastehfar et al. [8] has suggested that Pichai myanmarensis [9] should be transferred to the genus Wickerhamomyces, but W. myanmarensis has remained invalidly published. Therefore, in this study, we have proposed the validation of this name for a new combination species. In this case, 35 type species of Wickerhamomyces and P. myanmarensis have been reported since 1891. It has been revealed that the highest number of the Wickerhamomyces species were discovered during the period of 2011–2020, followed by the period of 1951–1960 (6 species), the period of 1971–1980 (4 species) and the period of 2001–2010 (3 species) (Figure 1). An increasing trend with regard to the discovery of new species of Wickerhamomyces is expected to continue in the future. Wickerhamomyces species are widely distributed in tropical, subtropical, temperate and subpolar areas throughout the world (Figure 2). It has been reported that the highest number of Wickerhamomyces species were found in Asia (18 species), followed by Europe (12 species), South America (8 species), Africa (7 species), North America (3 species), Oceania (1 species) and Antartica (1 species) [7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82] (Table 1). Both W. anomalus and W. onychis are known to be from Asia, Africa, Europe and South America [13−35,54−59]. Moreover, W. anomalus and W. rabaulenis have been discovered in Antarctica (King George Island) [17] and Oceania (Papua New Guinea) [67], respectively. Recently, W. psychrolipolyticus has been discovered from Japan [65]. Consequently, Wickerhamomyces species have been successfully isolated from various habitats, as has been summarized in Table 1.
Many species of Wickerhamomyces have been used in a variety of industries including the medicinal, agricultural, biofuel, food and beverage industries, and a number of others [64]. Most previous studies have focused on different strains of W. anomalus for biotechnological applications. For example, the W. anomalus strains CBS261, HN006 and HN010 are capable of excessively producing ethyl acetate. As a result, this species has been used in the brewing of Baijiu (Chinese liquor) and in winemaking to improve the aroma and quality of the finished product [83,84,85].
Wickerhamomyces anomalus strains BS91 and DMKU-RP04 could effectively inhibit plant pathogenic fungi and have been used as a biological control agent in agriculture [86,87,88]. Notably, W. anomalus strains SDBR-CMU-S1-06 and Wa-32 have exhibited plant growth promotion potential by solubilizing insoluble minerals, producing indole-3-acitic acid (IAA) and siderophores, and by secreting various extracellular enzymes [16,89]. Moreover, most strains of W. anomalus are known to produce killer toxins that possess antimicrobial and larvicidal activities [90,91]. Wickerhamomyces bovis and W. silvicola have been observed to produce mycocin, which exhibited fungicidal activity [92,93]. In addition, W. lynferdii and W. sydowiorum have been recognized as relevant yeast species for the improvement of the fermentation processes for coffee cherries and cocoa, respectively [79,94]. Furthermore, W. subpelliculosus has been used as an alternative to baker’s yeast [95], while W. chambardii could produce amylase and cellulase enzymes that could be used to produce bioethanol from corn straw [96,97]. Previous studies have found that the biosurfactants produced by W. anomalus and W. edaphicus [47,98,99], the saturated fatty acids produced by W. siamensis [100], xylitol produced by W. rabaulensis [101], cellulase enzymes produced by W. psychrolipolyticus [65] and the extracellular polysaccharide produced by W. mucosus [102] could be applied in the bioremediation, biotechnological and cosmetic industries. Furthermore, these substances could also be employed in the production of detergents, food and various pharmaceuticals, as well as in the process of oil recovery enhancement.
On the other hand, some Wickerhamomyces species (e.g., W. anomalus and W. lynferdii) have been responsible for the spoilage of beer and bakery products [103,104,105,106]. Some cases of human infection caused by W. anomalus, W. myanmarensis and W. onychis have also been reported, but only with patients with serious illness [8,107,108,109,110,111]. Based on this evidence, W. anomalus has been labeled a biosafety level 1 organism by the European Food Safety Authority [112] and is considered safe for consumption by healthy individuals.
Currently, only eight Wickerhamomyces species, namely W. anomalus, W. ciferrii, W. edaphicus, W. rabaulensis, W. siamensis, W. sydowiorum, W. tratensis and W. xylosicus, have been reported in Thailand [4,8,16,17,19,20,46,70,78]. Accordingly, Thailand has been identified as a hotspot for unexpected novel species and the newly recorded discovery of many microorganisms [113,114]. In our previous investigation on yeasts in soil samples collected from Assam tea (Camellia sinensis var. assamica) plantations in northern Thailand [16], we obtained five yeast strains belonged to the genus Wickerhamomyces that represent potentially new species. In our present study, we have described them into two novel species. These two novel species are introduced based on their phenotypic (morphological, biochemical and physiological data) and molecular characteristics. To confirm their taxonomic status, phylogenetic relationship was determined by analysis of the combined sequence dataset of the D1/D2 domains of LSU and ITS sequences.

2. Materials and Methods

2.1. Yeast Strain

Five yeasts strains (SDBR-CMU-S2-02, SDBR-CMU-S2-06, SDBR-CMU-S2-14, SDBR-CMU-S2-17 and CMU-S3-15) isolated from soils of Assam tea (C. sinensis var. assamica) plantations in Thep Sadej, Doi Saket District, Chiang Mai Province and Sri Na Pan, Muang District, Nan Province, northern Thailand [16] were selected for this present study. All strains were deposited in the culture collection of the Sustainable Development of Biological Resources, Faculty of Science, Chiang Mai University (SDBR-CMU), Chiang Mai Province and Thailand Bioresource Research Center (TBRC), Pathum Thani Province, Thailand.

2.2. Yeast Identification

2.2.1. Morphological Study

The morphological characteristics of yeast strains were determined according to established methods by Kurtzman et al. [2], Yarrow [3] and Limtong et al. [10]. Colony characters were observed on yeast extract-malt extract agar (YMA) after two days of incubation in darkness at 30 °C. Ascospore formation was investigated on YMA, 5% malt extract agar (MEA), potato dextrose agar (PDA) and V8 agar after incubation at 25 °C in the dark for four weeks. Micromorphological characteristics were examined under a light microscope (Nikon Eclipse Ni U, Tokyo, Japan). Size data of the anatomical structure (e.g., cells, pseudohyphae, asci and ascospores) were based on at least 50 measurements of each structure using the Tarosoft (R) Image Frame Work program.

2.2.2. Biochemical and Physiological Studies

Biochemical and physiological characterizations of yeast strains was followed the previous studies [2,3,115]. Fermentation of carbohydrates including glucose, galactose, maltose, sucrose, trehalose, melibiose, lactose, raffinose, and xylose were performed. Additionally, assimilation tests for carbon (D-glucose, D-galactose, L-sorbose, N-acetyl glucosamine, D-ribose, D-xylose, L-arabinose, D-arabinose, rhamnose, sucrose, maltose, α,α-trehalose, α-methyl-D-glucoside, cellobiose, salicin, melibiose, lactose, raffinose, melezitose, inulin, soluble starch, glycerol, erythritol, ribitol, D-glucitol, D-mannitol, galactitol, myo-inositol, D-glucono-1,5-lactone, 2-ketogluconic acid, 5-ketogluconic acid, D-gluconate, D-glucuronate, D-galacturonic acid, DL-lactate, succinate, citrate, methanol, ethanol, and xylitol) and nitrogen compounds (ammonium sulfate, potassium nitrate, sodium nitrite, ethylamine hydrochloride, L-lysine, cadaverine, and creatine) were determined. Moreover, the effects of temperature on growth were examined by cultivation on YMA at temperature ranging from 15–45 °C and diazonium blue B reactions were tested [116].

2.2.3. Molecular Study

Each yeast strain was grown in 5 mL of yeast extract-malt extract broth in 18 × 180 mm test tubes with shaking at 150 rpm on an orbital shaker in the dark for two days. Yeast cells were harvested by centrifugation at 11,000 rpm and washed three times with sterile distilled water. Genomic DNA was extracted from yeast cells using DNA Extraction Mini Kit (FAVORGEN, Taiwan) following the manufacturer’s protocol. The ITS region and D1/D2 domains of LSU gene were amplified by polymerase chain reactions (PCR) using ITS1/ITS4 primers [117] and NL1/NL4 primers [118], respectively. The amplification of both D1/D2 domains and ITS region process consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 52 °C for 45 s, an extension at 72 °C for 1 min and 72 °C for 10 min on a peqSTAR thermal cycler (PEQLAB Ltd., UK). PCR products were checked and purified by a PCR clean up Gel Extraction NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Germany). Final PCR products were sent to 1st Base Company Co., Ltd., (Kembangan, Malaysia) for sequencing. The obtained sequences were used to query GenBank via BLAST (http://blast.ddbj.nig.ac.jp/top-e.html, accessed on 25 August 2021).
Phylogenetic analysis was carried out based on the combined dataset of ITS and D1/D2 domains of LSU sequences. Sequences from this study along with those obtained from previous studies and the GenBank database were selected and provided in Table 2. Multiple sequence alignment was performed using MUSCLE [119]. A combination of D1/D2 domains of LSU and ITS alignment was deposited in TreeBASE under the study ID number 28785. A phylogenetic tree was constructed under maximum likelihood (ML) and Bayesian inference (BI) methods. The ML analysis was carried out using RAxML-HPC2 on XSEDE (8.2.10) in CIPRES Science Gateway V. 3.3 [120] using GTRCAT model with 25 categories and 1000 bootstrap (BS) replications. The optimum nucleotide substitution model was obtained using jModeltest v.2.3 [121] under the Akaike information criterion (AIC) method. The BI analysis was performed using MrBayes 3.2.6 software for Windows [122]. The selected optimal model of each gene is similar as GTR + I + G model. Six simultaneous Markov chains were run with one million generations and starting from random trees and keeping one tree every 100th generation until the average standard deviation of split frequencies was below 0.01. The value of burn-in was set to discard 25% of trees when calculating the posterior probabilities. Bayesian posterior probabilities (PP) were obtained from the 50% majority rule consensus of the trees kept. The tree topologies were visualized in FigTree v1.4.0 [123].

3. Results

3.1. Phylogenetic Results

The sequences of five yeast strains were deposited in the GenBank database (Table 2). The alignment of a combination of ITS and D1/D2 domains of the LSU genes contained 1544 characters including gaps (ITS: 1−823 and D1/D2 domains of LSU: 824−1544). RAxML analysis of the combined dataset yielded a best scoring tree with a final ML optimization likelihood value of −12,120.4323. The matrix contained 776 distinct alignment patterns with 42.33% undetermined characters or gaps. Estimated base frequencies were recorded as follows: A = 0.2730, C = 0.1821, G = 0.2603, T = 0.2844; substitution rates AC = 1.0574, AG = 2.0209, AT = 1.4684, CG = 0.6712, CT = 4.4165, GT = 1.0000. The gamma distribution shape parameter alpha was equal to 0.2698 and the Tree-Length was equal to 4.4075. In addition, the final average standard deviation of the split frequencies at the end of the total MCMC generations was calculated as 0.00638 through BI analysis. Phylograms of the ML and BI analyses were similar in terms of topology (data not shown). Therefore, the phylogram obtained from the ML analysis was selected and presented for this study. The phylogram was comprised of 67 sequences of Wickerhamomyces strains (including 37 type strains obtained from either previous studies or the present study) and two sequences (Saccharomyces cerevisiae NRRL 12632 and Spathaspora allomyrinae CBS 13924) of the outgroup (Figure 3). Our phylogenetic analysis separated Wickerhamomyces by different species based on different topologies. Our analysis confirmed that W. myanmarensis (previously known as P. myanmarensis) belonged to the genus Wickerhamomyces according to the phylogenetic results of Arastehfar et al. [8] and Shimizu et al. [65]. Moreover, a phylogram clearly separated our yeast strains into two monophyletic clades with high support values (BS = 100% and PP = 1.0). The results indicated that our two yeast strains, SDBR-CMU-S2-17 and SDBR-CMU-S2-14 (introduced as W. nanensis), were clearly distinguished from the previously known species of Wickerhamomyces. Moreover, three yeast strains in this study, SDBR-CMU-S2-02, SDBR-CMU-S2-15, and CMU-S3-06 (described here as W. lannaensis) formed a sister clade to W. ochangensis with high support (BS = 100% and PP = 1.0).

3.2. Taxonomic Description of New Species

3.2.1. Wickerhamomyces lannaensis S. Nundaeng, J. Kumla, N. Suwannarach and S. Lumyong, sp. nov. (Figure 4)

Mycobank No.: 841356
Etymology: “lannaensis” refers to Lanna kingdom the historic name of northern Thailand, the collection locality of the type strain of the species.
Holotype: Thailand, Chiang Mai Province, Thep Sadej, Doi Saket District, in soil from Assam tea (C. sinensis var. assamica) plantation, May 2017, J. Kumla and N. Suwannarach, (holotype SDBR-CMU-S3-15T, culture ex-type TBRC 15533)
Description: The streak culture on YMA after two days at 30 °C is circular from (1–2 mm in diameter), white to cream color, smooth surface, dull-shining, entire margin, and raised elevation. After growth on YMA at 30 °C for two days, the cells are spheroidal to short ovoidal (3.6–3.8 × 2.4–2.6 µm), occur singly or in budding pairs. Pseudohyphae and true hyphae were absent. Ascospores were not obtained for individual strains and strain pairs on YMA, 5% MEA, PDA and V8 agar after incubation at 30 °C for one month. Urea hydrolysis and diazonium blue B reactions are negative. Fermentation tests, glucose is delayed positive, but galactose, maltose, sucrose, trehalose, melibiose, lactose, raffinose, and xylose are negative. D-glucose, D-xylose, rhamnose, cellobiose, salicin, inulin (weak), glycerol, D-glucitol, D-mannitol, D-glucono-1,5-lactone, D-gluconate, DL-lactate (weak), succinate, and ethanol are assimilated. No growth was observed in L-sorbose, N-acetyl glucosamine, D-ribose, L-arabinose, D-arabinose, sucrose, maltose, α,α-trehalose, α-methyl-D-glucoside, melibiose, lactose, raffinose, melezitose, soluble starch, erythritol, ribitol, galactitol, myo-inositol, 2-ketogluconic acid, 5-ketogluconic acid, D-glucuronate, D-galacturonic acid, citrate, methanol, and xylitol. For the assimilation of nitrogen compounds, growth on ammonium sulfate, potassium nitrate, sodium nitrite, ethylamine HCl, cadaverine, and creatine (weak) are positive and on L-lysine is latent positive.
Growth in the vitamin-free medium is weak positive. Growth was observed at 15 °C and 30 °C, but not at 35, 37, 40, 42 and 45 °C. Growth in the presence of 50% glucose is positive, but growth in the presence of 0.01% cycloheximide, 0.1% cycloheximide, 60% glucose, 10% NaCl with 5% glucose and 15% NaCl with 5% glucose are negative. Acid formation is negative.
Additional strains examined: Thailand, Nan Province, Muang District, Sri Na Pan, in soil from Assam tea (C. sinensis var. assamica) plantation, September 2016, J. Kumla and N. Suwannarach, SDBR-CMU-S2-02, SDBR-CMU-S2-06.
GenBank accession numbers: holotype SDBR-CMU-S3-15 (D1/D2: MT639220, ITS: OK135750); additional strains SDBR-CMU-S2-02 (D1/D2: MT623569, ITS: OK135752) and SDBR-CMU-S2-06 (D1/D2: MT613722, ITS: OK135753).
Note: Based on phylogenetic analyses, W. lannaensis formed a monophyletic clade in a well-supported clade and was found to be closely related to W. ochangensis (Figure 3). Wickerhamomyces lannaensis can be distinguished from W. ochangensis by its ability to assimilate inulin and creatine and its growth in 50% glucose medium [11]. Additionally, W. ochangensis was able to grow at a temperature of 37 °C, while W. lannaensis could not grow at 37 °C [11].

3.2.2. Wickerhamomyces nanensis J. Kumla, S. Nundaeng, N. Suwannarach and S. Lumyong, sp. nov. (Figure 5)

Mycobank No.: 841357
Etymology: “nanensis” refers to Nan Province of Thailand, the collection locality of the type strain of the species.
Holotype: Thailand, Nan Province, Muang District, Sri Na Pan, in soil from Assam tea (C. sinensis var. assamica) plantation, September 2016, J. Kumla, N. Suwannarach and S. Khuna, (holotype SDBR-CMU-S2-17T, culture ex-type TBRC 15534)
Description: The streak culture on YMA after two days at 30 °C is circular from (1–2 mm in diameter), white to cream color, smooth surface, dull-shining, entire margin, and raised elevation. After growth on YMA at 30 °C for two days, the cells are spheroidal to short ovoidal (3.8–4.0 × 2.4–2.5 µm), occur singly or in budding pairs. Pseudohyphae (4.8–6.9 × 2.2–2.9 µm) were produced in Dalmau plate culture on 5% MEA and PDA after 7 days at 25 °C, but true hyphae are not obtained. Ascospores were not observed for individual strains and strain pairs on YMA, 5% MEA, PDA and V8 agar after incubation at 30 °C for one month. Urea hydrolysis and diazonium blue B reactions are negative. Fermentation test, glucose is delayed positive, but galactose, maltose, sucrose, trehalose, melibiose, lactose, raffinose, and xylose are not positive. D-glucose, D-galactose, cellobiose, salicin, glycerol, D-mannitol, D-glucono-1,5-lactone, DL-lactate (weak), succinate, citrate, and ethanol are assimilated. No growth was observed in L-sorbose, N-acetyl glucosamine, D-ribose, D-xylose, L-arabinose, D-arabinose, rhamnose, sucrose, maltose, α,α-trehalose, α-methyl-D-glucoside, melibiose, lactose, raffinose, melezitose, inulin, soluble starch, erythritol, ribitol, D-glucitol, galactitol, myo-inositol, 2-ketogluconic acid, 5-ketogluconic acid, D-gluconate, D-glucuronate, D-galacturonic acid, methanol, and xylitol. For the assimilation of nitrogen compounds, growth on ammonium sulfate, potassium nitrate (weak), sodium nitrite (weak), ethylamine HCl, l-lysine, and creatine (slow) are positive, but cadaverine is not. Growth in the vitamin-free medium is weak. Growth was observed at 15 °C and 30 °C, but not at 35, 37, 40, 42 and 45 °C. Growth in the presence of 50% glucose and acid formation are positive, but growth in the presence of 0.01% cycloheximide, 0.1% cycloheximide, 60% glucose, 10% NaCl with 5% glucose, and 15% NaCl with 5% glucose are negative.
Additional strain examined: Thailand, Nan Province, Muang District, Sri Na Pan, in soil from Assam tea (C. sinensis var. assamica) plantation, September 2016, J. Kumla and N. Suwannarach, SDBR-CMU-S2-14.
GenBank accession numbers: holotype SDBR-CMU-S2-17 (D1/D2: MT613875, ITS: OK143510); additional strain SDBR-CMU-S2-14 (D1/D2: MT623569, ITS: OK143511).
Note: Several morphological and biochemical characteristics of W. nanensis were similar to W. chambardii. However, W. chambardii differed from W. nanensis by its ascospore formation and could not assimilate D-mannitol [2]. Phylogenetic analyses clearly separated W. nanensis and W. chambardii as different species. Moreover, W. nanensis formed a monophyletic clade in a well-supported clade and was separated from other Wickerhamomyces species (Figure 3).

3.3. New Combination

Wickerhamomyces myanmarensis (Nagats., H. Kawas. and T. Seki) J. Kumla, N. Suwannarach and S. Lumyong, comb. nov.
Mycobank No.: 841356
Basionym: Pichia myanmarensis Nagats., H. Kawas. and T. Seki, Int. J. Syst. Evol. Microbiol. 55: 1381, 2005.
Note: The combined ITS and D1/D2 phylogenetic analyses indicated that the type species, P. myanmarensis, belongs to the genus Wickerhamomyces and has a close phylogenetic relationship with W. anomalus (Figure 3). Accordingly, the phylogenetic results of Arastehfar et al. [8] and Shimizu et al. [65] found that P. myanmarensis was placed within the genus Wickerhamomyces.

3.4. Key to Species of Wickerhamomyces

A key to the identification of the Wickerhamomyces species introduced in the present study was derived from the key described by Kurtzman et al. [2]. Key characteristics are shown in Table 3.
1.
a. Melibiose is assimilated………………...……………………..…..…..…………………2
   
b. Melibiose is not assimilated………..……………………………..…..….…….….….…7
2.
(1) a. Raffinose is assimilated………….…….…..…….….….….….……..………………3
      
b. Raffinose is not assimilated………………….…………………...….W. kurtzmanii
3.
(2) a. Citrate is assimilated………………………….……….………………….…………4
      
b. Citrate is not assimilated…………………….…….……………......….W. orientalis
4.
(3) a. Ribitol is assimilated………………...………….……………………………………5
      
b. Ribitol is not assimilated………………...………………………..…W. spegazzinii
5.
(4) a. Growth at 37 °C……………………………………………….….…….W. edaphicus
      
b. Growth is absent at 37 °C…………………………...…………..…….……………6
6.
(5) a. Ascospores observed on 5% MEA……………………………....…W. sydowiorum
      
b. Ascospores not observed on 5% MEA…………….………..…….…W. arborarius
7.
(1) a. Raffinose is assimilated…………………………….….……………………………8
      
b. Raffinose is not assimilated………………………………..………..…………….19
8.
(7) a. Nitrate is assimilated………………….……………………….………...………….9
      
b. Nitrate is not assimilated…………………………………………………….……15
9.
(8) a. L-Rhamnose is assimilated……………………………………………….……….10
         
b. L-Rhamnose is not assimilated………………………….………………………..12
10.
(9) a. L-Arabinose is assimilated…………………………..………………...….W. ciferrii
          
b. L-Arabinose is not assimilated…………………………..………………….……11
11.
(10) a. Sucrose is assimilated……………………………...……….W. psychrolipolyticus
          
b. Sucrose is not assimilated…………………………….……...…...W. xylosivorus
12.
(9) a. Growth in vitamin-free medium…………………………………………………13
        
b. Growth is absent in vitamin-free medium……….…..………..W. subpelliculosus
13.
(12) a. Soluble starch is assimilated…………………...……………………………….14
       
b. Soluble starch is not assimilated………………………….………....W. lynferdii
14.
(13) a. D-Arabinose is assimilated……………..……..….……………W. myanmarensis
       
b. D-Arabinose is not assimilated…………………..…………………W. anomalus
15.
(8) a. Ribitol is assimilated…………………………………………………………….16
       
b. Ribitol is not assimilated…………………………………….…………….……17
16.
(15) a. Galactose is assimilated……………………….....…………….W. strasburgensis
       
b. Galactose is not assimilated……………………..………..………W. rabaulensis
17.
(15) a. Growth in vitamin-free medium……….………....…………..…W. patagonicus
       
b. Growth is absent in vitamin-free medium…………………….……………...18
18.
(17) a. Citrate is assimilated……………………….…………...….….………W. onychis
       
b. Citrate is not assimilated………………………..…………..………W. siamensis
19.
(7) a. 2-Keto-D-gluconate is assimilated……………………………………….……….20
     
b. 2-Keto-D-gluconate is not assimilated……………………………...……………21
20.
(19) a. D-Glucitol is assimilated…………………………………….……….W. mucosus
       
b. D-Glucitol is not assimilated……………………….……………….W. xylosicus
21.
(19) a. D-Arabinose is assimilated………………………………..………………………22
       
b. D-Arabinose is not assimilated…………………………..…………………….23
22.
(21) a. Growth at 37 °C……….………………...………………………...…….W. sylviae
       
b. Growth is absent at 37 °C……………….…………….………………….W. mori
23.
(21) a. Galactose is assimilated……………………………………..…………...……..24
       
b. Galactose is not assimilated………………...…………………....…………….27
24.
(23) a. L-Arabinose is assimilated……………….……………………..…….W. silvicola
       
b. L-Arabinose is not assimilated………………...…………………....…...…….25
25.
(24) a. Sucrose is assimilated…………….………………….………….W. scolytoplatypi
       
b. Sucrose is not assimilated…………………….………………..……….………26
26.
(24) a. D-Mannitol is assimilated……………………..………….…….……W. nanensis
       
b. D-Mannitol is not assimilated………………..….……….…....….W. chambardii
27.
(23) a. L-Sorbose is assimilated………………….……...…..…………..……...W. pijperi
       
b. L- Sorbose is not assimilated……………………….………….….……………28
28.
(27) a. D-Xylose is assimilated……………………………………………...………….29
       
b. D-Xylose is not assimilated……………….…………..….………….W. tratensis
29.
(28) a. Sucrose is assimilated……………………………...……………………………30
       
b. Sucrose is not assimilated……………………………...……………….………36
30.
(29) a. Cellobiose is assimilated……………………………………..………………....31
       
b. Cellobiose is not assimilated…………..…………………………….W. queroliae
31.
(30) a. D-Glucitol is assimilated…………………………………………….………….32
       
b. D-Glucitol is not assimilated…………………….…………….W. chaumierensis
32.
(31) a. Growth at 37 °C………………………………….……………………………....33
       
b. Growth is absent at 37 °C………………………………...…………….………34
33.
(32) a. L-Arabinose is assimilated……………………….....………..………….W. bovis
       
b. L-Arabinose is not assimilated……………..…………….……….W. canadensis
34.
(32) a. Nitrate is assimilated……………………………………………...…………….35
       
b. Nitrate is not assimilated…………………………..……...…...W. hampshirensis
35.
(34) a. True hyphae are formed……………..……………………………….W. bisporus
       
b. True hyphae are not formed…………………………………...…………W. alni
36.
(29) a. Citrate is assimilated……………………………...……..………..W. menglaensis
       
b. Citrate is not assimilated……………………………….………………………37
37.
(36) a. Growth at 37 °C……………………..………..……..…..…………W. ochangensis
       
b. Growth is absent at 37 °C………………..………...…..…….…….W. lannaensis

4. Discussion

Traditional methods of identification and characterization for the Wickerhamomyces species are based primarily on phenotypical characteristics. These are further recognized as relevant morphological, biochemical, and physiological characteristics [41,128]. However, identification can be difficult because some species have similar appearances, and some biochemical characteristics are consistent across a number of species. In accordance with this evidence, previous species of Wickerhamomyces were originally classified into various yeast genera [1,2,4,5,50,70,75]. In 2008, the genus Wickerhamomyces was proposed by Kurtzman et al. [1], wherein this genus was clearly separated from other yeast genera based on phylogenetic evidence. Subsequently, some previously identified species were then transferred from the genera Candida, Hansenula, Pichia, and Williopsis [1,4,5,50,54,70]. Therefore, molecular phylogenetic analysis is necessary to concretely identify the Wickerhamomyces species. Species of the genus Wickerhamomyces are known to be widely distributed throughout the world and have been isolated from various habitats as shown in Table 1. Prior to conducting our study, Wickerhamomyces consisted of 35 accepted and published species according to molecular phylogenetic analysis. Our phylogenetic results were similar to those of Arastehfar et al. [8] and Shimizu et al. [65] who indicated that P. myanmarensis should be placed in the genus Wickerhamomyces. Consequently, we have proposed that this yeast species be named W. myanmarensis.
Yeast diversity has been investigated in various habitats throughout different regions of Thailand [5,10,15,16,18,19,46,70,78]. Wickerhamomyces anomalus was first species reported in Thailand in 2002 [20]. In 2009, the first new species, W. edaphicus has been discovered in Thailand [10]. Until now, a total of eight Wickerhamomyces species have been found [5,10,20,46,68,70,78,82]. However, W. siamensis, W. tratensis, and W. xylosicus were only known to be from Thailand [5,70,82]. In this study, two new Wickerhamomyces species, namely W. lannaensis and W. nanensis, that were isolated from soil collected from Assam tea plantations in northern Thailand were proposed based on identification through molecular phylogenetic and phenotypic (morphological, biochemical, and physiological characteristics) analyses. Therefore, effective identification of the Wickerhamomyces species has increased the number of species found in Thailand to 10 species and has led to 38 global species. This present discovery has increased the number of species of yeast known to be from Thailand and is considered important in terms of stimulating deeper investigations of yeast varieties in Thailand. Ultimately, these findings will help researchers gain a better understanding of the distribution and ecology of Wickerhamomyces.
Many species of the genus Wickerhamomyces have been investigated, and some strains have been used in a variety of biotechnology, food, and beverage industries, as well as in medical and agricultural fields [83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102]. Despite the fact that many Wickerhamomyces species can survive in a variety of environments, climate change has had an impact on both the terrestrial biome and the aquatic environment. These environments are known to serve as habitats for a number of microorganisms [130,131,132,133,134] and may have an impact on the global diversity and distribution of Wickerhamomyces. Therefore, in addition to studying the diversity and distribution of newly identified species, future research should focus on the effects of climate change on Wickerhamomyces.

Author Contributions

Conceptualization, S.N., J.K., N.S. and S.L. (Saisamorn Lumyong); methodology, S.N., J.K., N.S. and S.L. (Savitree Limtong); software, N.S. and J.K.; validation, N.S., J.K., S.L. (Savitree Limtong) and S.L. (Saisamorn Lumyong); formal analysis, S.N., J.K., N.S.; investigation, S.N., J.K. and N.S.; resources, J.K., N.S. and S.K.; data curation, N.S., J.K. and N.S.; writing—original draft, S.N., J.K. and N.S.; writing—review and editing, J.K., S.N., N.S., S.L. (Savitree Limtong) and S.L. (Saisamorn Lumyong); supervision, S.L. (Saisamorn Lumyong). All authors have read and agreed to the published version of the manuscript.

Funding

The authors gratefully acknowledge the financial support provided from Chiang Mai University, Thailand.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The DNA sequence data obtained from this study have been deposited in GenBank under accession numbers; D1/D2 domains (MT639220, MT623569, MT613722, MT613875 and MT623569) and ITS (OK135750, OK135752, OK135753, OK143510 and OK143511).

Acknowledgments

The authors are grateful to Russell Kirk Hollis for kind help in the English correction.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The discovering of Wickerhamomyces type species since 1891 till the present time.
Figure 1. The discovering of Wickerhamomyces type species since 1891 till the present time.
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Figure 2. Global distribution of Wickerhamomyces species. The countries where Wickerhamomyces species have been discovered are indicated in orange color.
Figure 2. Global distribution of Wickerhamomyces species. The countries where Wickerhamomyces species have been discovered are indicated in orange color.
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Figure 3. Phylogram derived from maximum likelihood analysis of 69 sequences of the combined ITS and D1/D2 sequences. Saccharomyces cerevisiae NRRL 12632 and Spathaspora allomyrinae CBS 13924 were used as the outgroup. The numbers above branches represent bootstrap percentages (left) and Bayesian posterior probabilities (right). Bootstrap values ≥ 75% and Bayesian posterior probabilities ≥ 0.90 are shown. Sequences obtained in this study are in red. Superscription “T” means the type strains.
Figure 3. Phylogram derived from maximum likelihood analysis of 69 sequences of the combined ITS and D1/D2 sequences. Saccharomyces cerevisiae NRRL 12632 and Spathaspora allomyrinae CBS 13924 were used as the outgroup. The numbers above branches represent bootstrap percentages (left) and Bayesian posterior probabilities (right). Bootstrap values ≥ 75% and Bayesian posterior probabilities ≥ 0.90 are shown. Sequences obtained in this study are in red. Superscription “T” means the type strains.
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Figure 4. Wickerhamomyces lannaensis (holotype SDBR-CMU-S3-15). Culture (a), single colony (b) and budding cells (c) on YMA after two days at 30 °C. Scale bar a and b = 10 cm, c = 10 µm.
Figure 4. Wickerhamomyces lannaensis (holotype SDBR-CMU-S3-15). Culture (a), single colony (b) and budding cells (c) on YMA after two days at 30 °C. Scale bar a and b = 10 cm, c = 10 µm.
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Figure 5. Wickerhamomyces nanensis (holotype SDBR-CMU-S3-15). Culture (a), single colony (b) and budding cells (c) on YMA after two days at 30 °C. Pseudohypahae (d) on 5% MAE agar after 7 days at 25 °C. Scale bar a and b = 10 cm, c and d = 10 µm.
Figure 5. Wickerhamomyces nanensis (holotype SDBR-CMU-S3-15). Culture (a), single colony (b) and budding cells (c) on YMA after two days at 30 °C. Pseudohypahae (d) on 5% MAE agar after 7 days at 25 °C. Scale bar a and b = 10 cm, c and d = 10 µm.
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Table
Table 1. Global distribution and isolation sources of Wickerhamomyces species.
Table 1. Global distribution and isolation sources of Wickerhamomyces species.
No.SpicesKnown DistributionIsolation SourceReference
1Wickerhamomyces alni (Phaff, M.W. Mill. and M. Miranda) Kurtzman, Robnett and Bas.-PowersCanadaExudate of Alnus rubra[12]
2Wickerhamomyces anomalus (E.C. Hansen) Kurtzman, Robnett and Bas.-PowersAlgeria, Brazil,
China, Colombia, Ethiopia, India, Iraq, King George Island, Lao, Russia, Slovakia Sweden and Thailand		Process of beer and wine, phylloplane, soil, water, coral reefs, Thai traditional alcoholic starter, mangrove forest, fermented food, flowers, fruits, fermented grains, coffee processing, wastewater treatment plant, Colombian fermented beans and Brazilian spirit [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35]
3Wickerhamomyces arborarius S.A. James, E.J. Carvajal, Barahona, T.C. Harr., C.F. Lee, C.J. Bond and I.N. RobertsEcuadorFlower[36]
4Wickerhamomyces bisporus (O. Beck) Kurtzman, Robnett and Bas.-PowersFinland, France and USAPlatypus compositus, phoretic mites on Ips typographus and bark beetles (Dendroctonus)[37,38,39]
5Wickerhamomyces bovis (Uden and Carmo Souza) Kurtzman, Robnett and Bas.-PowersPortugalCaecum of feral cattle (Bos taurus)[40]
6Wickerhamomyces canadensis (Wick.) Kurtzman, Robnett and Bas.-PowersCanadaBeetle frass from Pinus resinosa[41]
7Wickerhamomyces chambardii (C. Ramírez and Boidin) Kurtzman, Robnett and Bas.-PowersFranceChestnut[42]
8Wickerhamomyces chaumierensis M. Groenew., V. Robert and M.T. Sm.GuyanaSurface of flower[43]
9Wickerhamomyces ciferrii (Lodder) Kurtzman, Robnett and Bas.-PowersDominican Republic, USA and ThailandFruit of Dipteryx odorata and male olive fruit fly (Bactrocera oleae)[44,45,46]
10Wickerhamomyces edaphicus Limtong, Yongman., H. Kawas. and FujiyamaIndia and ThailandForest and mangrove soils[4,47]
11Wickerhamomyces hampshirensis (Kurtzman) Kurtzman, Robnett and Bas.-PowersUSAFrass of cut and dead of Quercus and beetle (Xyloterinus politus)[48,49]
12Wickerhamomyces kurtzmanii A.H. Li, Y.G. Zhou and Q.M. WangChinaCrater lake water[6]
13Wickerhamomyces lynferdii (Van der Walt and Johannsen) Kurtzman, Robnett and Bas.-PowersSouth AfricaSoil[50]
14Wickerhamomyces menglaensis F.L. Hui and L.N. HuangChinaRotting wood[51]
15Wickerhamomyces mori F.L. Hui, Liang Chen, X.Y. Chu, Niu and T. KeChinaGut of larvae of wood-boring insect on trunk of Morus alba[52]
16Wickerhamomyces mucosus (Wick. and Kurtzman) Kurtzman, Robnett and Bas.-PowersUSASoil[53]
17Wickerhamomyces myanmarensis (Nagats., H. Kawas. and T. Seki) J. Kumla, N. Suwannarach and S. Lumyong Iran and MyanmarPalm sugar in rum distiller, and blood and central venous catheter of patients[8,9]
18Wickerhamomyces ochangensis K.S. ShinSouth KoreaSoil of potato field[11]
19Wickerhamomyces onychis (Yarrow) Kurtzman, Robnett and Bas.-PowersBrazil, Ethiopia, Iraq, Malaysia, Netherlands, Poland and TunisiaNail infection of Homo sapiens, fermented food, cocoa beans, grape and tomato during spontaneous fermentation, and soil[23,54,55,56,57,58,59]
20Wickerhamomyces orientalis Sipiczki, S. Nasr, H.D.T. Nguyen and SoudiIran and Sri LankaFruits and rhizosphere soil[27,60]
21Wickerhamomyces patagonicus V. de García, Brizzio, C.A. Rosa, Libkind and Van BroockArgentinaSap exudate on cut branches of Nothofagus dombeyi and glacier meltwater river[61]
22Wickerhamomyces pijperi (Van der Walt & Tscheuschner) Kurtzman, Robnett and Bas.-PowersEgypt, Ghana and South AfricaButtermilk, cocoa fermentation and orange juice[62,63,64]
23Wickerhamomyces psychrolipolyticus Y. Shimizu and K. KonnoJapanSoil[65]
24Wickerhamomyces queroliae C.A. Rosa, P.B. Morais, Lachance and PimentaBrazilLarva of Anastrepha mucronata from fruit of Peritassa campestris[66]
25Wickerhamomyces rabaulensis (Soneda and S. Uchida) Kurtzman, Robnett and Bas.-PowersEthiopia, Papua New Guinea and ThailandExcreta of snail, soils, decaying agricultural residues, decaying leaves and tree bark, and
fermented food		[23,67,68]
26Wickerhamomyces scolytoplatypi NinomiyaJapanGallery of beetles (Scolytoplatypus shogun) in Fagus crenata[69]
27Wickerhamomyces siamensis Kaewwich., Yongman., H. Kawas. and LimtongThailandPhylloplane of Saccharum officinarum[70]
28Wickerhamomyces silvicola (Wick.) Kurtzman, Robnett and Bas.-Powers Germany, South
Korea and USA		Flowers, gum of Prunus serotina and Prunus wood[41,71,72]
29Wickerhamomyces spegazzinii Masiulionis and PagnoccaArgentinaThe fungus garden of an attine ant nest (Acromyrmex lundii)[73]
30Wickerhamomyces strasburgensis (C. Ramírez and Boidin) Kurtzman, Robnett and Bas.-PowersFranceOn leather tanned by vegetable means[74]
31Wickerhamomyces subpelliculosus (Kurtzman) Kurtzman, Robnett and Bas.-PowersEgypt and USAFermenting cucumber brines, gut of honey bee and molasses[75,76]
32Wickerhamomyces sydowiorum (D.B. Scott and Van der Walt) Kurtzman, Robnett and Bas.-PowersBrazil, Ivory Coast, South Africa and
Thailand		Frass of Sinoxylon ruficorne in dead Combretum apiculatum, decayed plant leaf, fermented cocoa, honey, sand and water[59,77,78,79,80]
33Wickerhamomyces sylviae Moschetti and J.P. Samp.ItalyCloaca of migratory birds
(Sylvia communis)		[81]
34Wickerhamomyces tratensis Nakase, Jindam., Am-In, Ninomiya and H. Kawas.ThailandFlower of mangrove apple
(Sonneratia caseolaris)		[82]
35Wickerhamomyces xylosicus Limtong, Nitiyon, Kaewwich., Jindam., Am-In and YongmanThailandSoil[5]
36Wickerhamomyces xylosivorus R. Kobay., A. Kanti and H. Kawas.IndonesiaDecayed wood[4]
Table
Table 2. DNA sequences used in the molecular phylogenetic analysis. Type strains indicated by “T”.
Table 2. DNA sequences used in the molecular phylogenetic analysis. Type strains indicated by “T”.
Yeast SpeciesStrainGenBank Acession NumberReference
ITSD1/D2
Wickerhamomyces alniNRRL Y-11625T‒EF550294[1]
CBS 6986NR154966KY110065[124]
Wickerhamomyces anomalusNRRL Y-366T‒EF550341[1]
H1WhJQ857021JQ856997[17]
Wickerhamomyces arborariusBq 164TNR_55000FN908198[36]
Wickerhamomyces bisporusNRRL Y-1482T‒EF550296[1]
Wickerhamomyces bovisNRRL YB-4184T‒EF550298[1]
CBS 2616NR154968KY110109[124]
Wickerhamomyces canadensisNRRL Y-1888T‒EF550300[1]
GoToruMP327EF093299EF016107[125]
Wickerhamomyces chambardiiNRRL Y-2378T‒EF550344[1]
CBS 1900NR154969KY110114[124]
Wickerhamomyces chaumierensisCBS 8565THM156503HM156533[43]
Wickerhamomyces ciferriiNRRL Y-1031T‒EF550339[1]
UCDFST 83-22MH153583MH130275[126]
Wickerhamomyces edaphicusS-29TAB436771AB436763[10]
CBS 10408KY105904KY110120[124]
Wickerhamomyces hampshirensisNRRL YB-4128T‒EF550334[1]
Wickerhamomyces kurtzmaniiTF5-16-2TMK573939MK573939[6]
Wickerhamomyces lannaensisSDBR-CMU-S3-15TOK135750MT639220This study, [16]
SDBR-CMU-S2-02OK135752MT623569This study, [16]
SDBR-CMU-S2-06OK135753MT613722This study, [16]
Wickerhamomyces lynferdiiNRRL Y-7723TEF550342EF550342[1]
BCRC 22676NR111798‒[127]
Wickerhamomyces menglaensisNYNU 1673TKY213818KY213812[51]
Wickerhamomyces moriNYNU 1216TJX204288JX204287[52]
NYNU 1204JX292100JX292099[52]
Wickerhamomyces mucosusNRRL YB-1344T‒EF550337[1]
CBS 6341Z93877KY110124[124]
Wickerhamomyces myanmarensisCBS 9786T‒AB126678[9]
SU-263MH236221MH236219[8]
Wickerhamomyces nanensisSDBR-CMU-S2-17TOK143510MT613875This study, [16]
SDBR-CMU-S2-14OK143511MT623571This study, [16]
Wickerhamomyces ochangensisN7a-Y2TNR154971HM485464[11]
CBS 11843KY105909‒[124]
Wickerhamomyces onychisNRRL Y-7123T‒EF550279[1]
CBS 5587KY105910KY110125[124]
Wickerhamomyces orientalisKH-D1TKF938677KF938676[60]
12-101KU253704KU253703[60]
Wickerhamomyces patagonicusCRUB 1724TFJ793131FJ666399[61]
CBS 11398NG057185KY110126[124]
Wickerhamomyces pijperiNRRL YB-4309T‒EF550335[1]
CBS 2887HM156502KY110127[124]
Wickerhamomyces psychrolipolyticusY08-202-2T‒LC333101[65]
Y08-202-2‒LC333102[65]
Wickerhamomyces queroliaeUFMG-T05-200TEU580140EU580140[66]
Wickerhamomyces rabaulensisNRRL Y-7945T‒EF550303[1]
CBS 6797KY105914KY110128[124]
Wickerhamomyces scolytoplatypiNBRC 11029T‒AB534166[69]
CBS 12186KY105915KY110130[124]
Wickerhamomyces siamensisDMKU-RK359TNR111029AB714248[70]
CBS 12570KY105916KY110131[124]
Wickerhamomyces silvicolaNRRL Y-1678T‒EF550302[1]
GLMC 1708MT156140MT156324[71]
Wickerhamomyces spegazziniiJLU025TKJ832072KJ832071 [73]
Wickerhamomyces strasburgensisNRRL Y-2383T‒EF550333[1]
Wickerhamomyces subpelliculosusNRRL Y-1683TNR111336EF550340[1,127]
Wickerhamomyces sydowiorumNRRL Y-7130TNR138219EF550343[1,36]
NRRL Y-10996FR690145FR690073[36]
Wickerhamomyces sylviaePYCC6345T‒KF240728[81]
U92A1‒KF240729[81]
Wickerhamomyces tratensisNBRC 107799TAB607029AB607028[82]
CBS 12176KY105935KY110150[124]
Wickerhamomyces xylosicaCBS 12320TNR160310AB557867[5]
NT31AB704715NG064304[5]
Wickerhamomyces xylosivorusNBRC 111553TNR155013LC202858[4]
14Y125‒NG057186[4]
Saccharomyces cerevisiaeNRRL Y-12632TAY046146JQ689017[128]
Spathaspora allomyrinaeCBS 13924TKP054268KP054267[129]
Note: sepecies obtained in this study are in bold.
Table
Table 3. Key characteristics of species assigned to the genus Wickerhamomyces.
Table 3. Key characteristics of species assigned to the genus Wickerhamomyces.
SpeciesGrowth in/at *Ascospores
on 5% MEA 		True Hyphae
GaSorDXyLArDArRhSuCelMlbRafStRblDGluManGlt2-ketCitNO3‒V37 °C
W. alni‒‒+‒‒+++‒‒‒v++‒‒++‒‒+‒
W. anomalusv‒vv‒‒++‒++v++‒‒+++v+‒
W. arborarius+l/‒+vvl/++v++++++‒n++n‒‒‒
W. bisporus‒‒+w/‒‒+++‒‒‒vw/+v‒‒++‒‒n+
W. bovis‒‒++‒v++‒‒v‒++‒‒+‒‒++‒
W. canadensis‒‒+‒‒w/+++‒‒‒v+w/+‒‒+v‒++v
W. chambardii+‒‒‒‒‒‒+‒‒‒‒‒‒‒‒w‒‒‒+‒
W. chaumierensis‒‒+‒n‒++‒‒nn‒‒n‒n‒n‒n‒
W. ciferrii+‒w/+w/+‒w/++w/+‒+++++‒‒+++w/‒+v
W. edaphicus+‒+w/‒‒+++++++++l/‒‒+++wn‒
W. hampshirensis‒‒+‒‒s++‒‒‒w/++v‒‒s‒‒‒+‒
W. kurtzmanii‒‒‒‒‒‒+‒w‒‒‒‒w‒n‒+‒‒‒‒
W. lynferdii+‒‒‒‒‒++‒+‒+++‒‒+++‒+‒
W. menglaensis‒‒ww‒w‒+‒‒w‒++‒‒+++n‒‒
W. mori‒+‒‒w‒+‒‒‒‒‒n+‒‒w‒+‒‒‒
W. mucosus‒++‒v‒++‒‒+‒+w/+‒+‒‒‒‒+‒
W. myanmarensis+‒sws‒+s‒s++++‒n+++++‒
W. ochangensis‒‒+‒‒+‒+‒‒‒‒++‒‒‒+n+n‒
W. onychis‒‒+vv‒++‒+‒‒++‒‒+‒‒++‒
W. orientalis+nwwww/‒++ww‒wnwnn‒‒‒+‒+
W. patagonicusw‒+‒‒+nw‒ww‒w‒‒n‒‒+‒n ‒
W. pijperi‒++‒‒‒‒+‒‒‒‒++‒‒v‒‒‒+‒
W. psychrolipolyticus‒‒+‒++++‒++‒++nn++n‒‒‒
W. queroliae‒‒++‒++‒‒‒‒+++‒‒w/s+‒+‒‒
W. rabaulensis‒‒++‒v++‒+‒+++‒‒+‒‒++‒
W. scolytoplatypi+‒s‒‒s++‒‒+s++‒‒‒+‒‒+‒
W. siamensiss‒s‒‒‒+w‒ww‒ww‒‒‒‒‒++-
W. silvicola+v++‒+v+‒‒‒++v‒‒v+‒v+v
W. spegazzinii+‒+‒‒+++++s‒++‒‒w++++‒
W. strasburgensis+‒++‒+++‒+‒+++‒‒+‒‒v+‒
W. subpelliculosusv‒vvv‒+v‒+vv++‒‒++‒v+v
W. sydowiorum+‒v+‒+++++v+++‒‒+++‒+‒
W. sylviaev‒s/‒++ +,‒w/‒s/‒‒‒w/+s/‒‒‒‒‒‒v++‒‒
W. tratensis‒‒‒‒‒‒‒v‒‒‒‒vv‒‒‒nn+n‒
W. xylosicus‒++‒‒‒++‒‒‒‒‒+‒w‒‒n‒+‒
W. xylosivorusw/‒‒+‒‒+‒+‒w‒‒+‒n‒‒++n‒‒
W. lannaensis‒‒+‒‒+‒+‒‒‒‒++‒‒‒+w‒‒‒
W. nanensis+‒‒‒‒‒‒+‒‒‒‒‒+‒‒+ww‒‒‒
* Ga = Galactose, Sor = L-Sorbose, DXy = D-Xylose, LAr = L-Arabinose, DAr = D-Arabinose, Rh = L-Rhamnose, Su = Sucrose, Cel = Cellobiose, Mlb = Melibiose, Raf = Raffinose, St = Soluble starch, Rbl = Ribitol, DGlu = D-glucitol, Man = D-Mannitol, Glt = Galactitol, 2-ket = 2-ketogluconic acid, Cit = Citrate, NO3 = Potassium nitrate, ‒V = vitamin-free medium, 37 °C = Growth at 37 °C. “+” = strong growth or produce, “‒“ = absence of growth or not produce, “w” = weak growth, “v” = strain variable response, “l” = latent positive, “s” = slow positive, and “n” = no data.
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Nundaeng, S.; Suwannarach, N.; Limtong, S.; Khuna, S.; Kumla, J.; Lumyong, S. An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand. J. Fungi 2021, 7, 957. https://doi.org/10.3390/jof7110957

AMA Style

Nundaeng S, Suwannarach N, Limtong S, Khuna S, Kumla J, Lumyong S. An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand. Journal of Fungi. 2021; 7(11):957. https://doi.org/10.3390/jof7110957

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Nundaeng, Supakorn, Nakarin Suwannarach, Savitree Limtong, Surapong Khuna, Jaturong Kumla, and Saisamorn Lumyong. 2021. "An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand" Journal of Fungi 7, no. 11: 957. https://doi.org/10.3390/jof7110957

APA Style

Nundaeng, S., Suwannarach, N., Limtong, S., Khuna, S., Kumla, J., & Lumyong, S. (2021). An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand. Journal of Fungi, 7(11), 957. https://doi.org/10.3390/jof7110957

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