Next Article in Journal
Progress in Fermented Unconventional Feed Application in Monogastric Animal Production in China
Next Article in Special Issue
Oxidative Phosphorylation for Aerobic Survival, but Not for Growth: The Peculiar ‘Make-Accumulate-Consume’ Strategy in Zymomonas mobilis
Previous Article in Journal
Improvement of Laccase Activity in Co-Culture of Panus lecomtei and Sporidiobolus pararoseus and Its Application as an Enzymatic Additive in Biomass Hydrolysis and Dye Decolorization
Previous Article in Special Issue
Bioacetoin Production by Bacillus subtilis subsp. subtilis Using Enzymatic Hydrolysate of Lignocellulosic Biomass
 
 
Article
Peer-Review Record

Effect of Blue LED Light on Bioemulsifier Production in Bioreactor by Aureobasidium pullulans LB83 in Solid State Fermentation

Fermentation 2023, 9(11), 946; https://doi.org/10.3390/fermentation9110946
by Daylin Rubio-Ribeaux 1, Rogger Alessandro Mata da Costa 1, Renan Murbach Pereira 1, Paulo Ricardo Franco Marcelino 1, Fernanda Perpétua Casciatori 2, Júlio César dos Santos 1 and Silvio Silvério da Silva 1,*
Reviewer 1:
Reviewer 2:
Fermentation 2023, 9(11), 946; https://doi.org/10.3390/fermentation9110946
Submission received: 9 October 2023 / Revised: 27 October 2023 / Accepted: 27 October 2023 / Published: 31 October 2023

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This manuscript demonstrated the effect of blue LED light on bioemulsifier production in bioreactor by Aureobasidium pullulans LB83 in SSF. It is a well presented. Minor revision is recommended. Authors should explain the reason of the superiorty of blue LED light to other studied LED lights. Maybe physology of Aureobasidium pullulans and some biochemical pathways could be discussed in more detail.

Author Response

Please see the attachment.

Comments from Reviewer 1

Comments 1: This manuscript demonstrated the effect of blue LED light on bioemulsifler production in bioreactor by Aureobasidium pullulans LB83 in SSF. It is a well presented. Minor revision is recommended. Authors should explain the reason of the superiorty of blue LED light to other studied LED lights. Maybe physology of Aureobasidium pullulans and some biochemical pathways could be discussed in more detail.

Response 1: Dear Reviewer, thank you for pointing this out. We agree with this comment. In the article, a general mechanism has been proposed to shed light on the activation of metabolic pathways involved in the synthesis of both bioemulsifier and pigments. This proposed pathway is derived from the elucidation of genes that are common to the production of both bioemulsifier and melanin. However, it is noteworthy that, despite our rigorous examination, specific information elucidating the reason behind the prevalence of blue light as an activator could not be found. For this reason, we emphasize the imperative need for further studies.

Author Response File: Author Response.docx

Reviewer 2 Report

Comments and Suggestions for Authors

This study has found that the blue light is better to promote bioemulsifier production than other wavelengths of light. Overall, I think the study is interesting and the experimental data is sufficient to support the conclusion. My questions and suggestions are as follows.

1. Whats the mechanism by which the blue light can promote the growth of A. pullulans? I suggest the authors discuss this question in the introduction or discussion.

2. Does EI24% have the same meaning with IE24%? In line 362 and 363, why does IE24% appear?

3. All the figures should be beautified and clarified.

In Fig 1, the blue curves should be drawn more regularly. The brown part should be redrawn.

In Fig 2, the Y-axis title is wrong, E24% should be changed to EI24%. The kerosene column shouldn’t be filled with black so that the error bars cannot be seen.

The ordinate title of Fig 3 has the same problem as Fig 2. Besides, it’d better change change the position of the legend in the upper right corner to make sure the figure is clear and beautiful.

In Fig 4, the unit in the coordinate title is wrong, it should be changed to IUg-1.

In Fig 5, it’s recommended to mark the meaning of where the arrow points. 

Comments on the Quality of English Language

There are some formatting and spelling errors, please check the whole manuscript carefully.

Author Response

Please see the attachment.

Comments 1: What’s the mechanism by which the blue light can promote the growth of A. pullulans? I suggest the authors discuss this question in the introduction or discussion.

Response 1: Dear reviewer, thank you for pointing this out. In the Results and Discussion section, we discussed a general mechanism aimed at elucidating the activation of metabolic pathways related to the synthesis of both the bioemulsifier and the pigment under the influence of light. However, it is relevant to highlight that, despite our thorough analysis, we did not find specific information that would clarify the underlying cause of the preference for blue light as the activating factor. Given this gap, we emphasize the need for further studies. The proposed approach was based on the identification of genes that are shared in the production of both metabolites, namely, the bioemulsifier and melanin. 




Comment 2: Does EI24% have the same meaning with IE24%? In line 362 and 363, why does IE24% appear?

Response 2: Dear reviewer, thank you for pointing this out. Accordingly, we have revised the term IE24% (which should be EI24%). Yes, they have the same meaning; therefore, we have already changed from IE24% to EI24% in lines 361 and 362. 


Comment 3: All the figures should be beautified and clarified. In Fig 1, the blue curves should be drawn more regularly. The brown part should be redrawn.

Response 3: Dear reviewer, thank you for pointing this out. We have redesigned the blue curves of the drawing in a more regular way, which is now green. We also redesigned the brown part, which symbolized the substrate (pretreated sugarcane bagasse) inside the bioreactor.


Comment 4: All the figures should be beautified and clarified. In Fig 2, the Y-axis title is wrong, E24% should be changed to EI24%. The kerosene column shouldn’t be filled with black so that the error bars cannot be seen.

Response 4: Dear reviewer, thank you for pointing this out. We changed the title of the Y axis from E24% to EI24% and the kerosene column drawn with another color so that the error bars can be seen in more detail. This change can be found on page 7, line 265.


Comment 5: All the figures should be beautified and clarified. The ordinate title of Fig 3 has the same problem as Fig 2. Besides, it’d better change change the position of the legend in the upper right corner to make sure the figure is clear and beautiful.


Response 5: Dear reviewer, thank you for pointing this out. We have, accordingly, revised the Y-axis title from E24% to EI24%. In addition, we have already changed the graph points to another color so that the error bars can be seen and the graph legends have been adjusted in the upper right corner, as requested by the reviewer. This change can be found on page 7, line 265. 


Response 5: Dear reviewer, thank you for pointing this out. We changed the Y axis title from E24% to EI24%. Additionally, we changed the graph points to another color so that the error bars can be better visualized and the graph legends were adjusted in the upper right corner, as requested by the reviewer. This change can be found on page 7, line 265.


Comment 6: All the figures should be beautified and clarified. In Fig 4, the unit in the coordinate title is wrong, it should be changed to IUg-1.


Response 6: Dear reviewer, thank you for pointing this out. We revised the unit of measurement in the coordinates title in Figure 4 and changed it from IUg- to IUg-1. We also changed the colors of endoglucanase and exoglucanase so that the error bars can be better visualized on the graph. This change can be found on page 11, line 398.


Comment 7: All the figures should be beautified and clarified. In Fig 5, it’s recommended to mark the meaning of where the arrow points. 


Response 7: Dear reviewer, thank you for pointing this out. The description of the image in Figure 5: "Scanning electron microscopy (SEM): (A) PSB at time 0 (before inoculation) (B) PSB at the end of the fermentation process (9 days) was modified in the text. The current description was as follows: "Scanning electron microscopy (SEM): (A) PSB at time 0 (before inoculation), with some micropores on the surface of the pre-treated biomass due to partial removal of hemicelluloses and lignin; (B) PSB at the end of the fermentation process (9 days) with the presence of yeast cells on the surface of the biomass". Furthermore, in the latest version, an explanation of the meaning of the arrow marked in Figure B was provided to facilitate better understanding for the readers. This change can be found on page 13, lines 462 to 465.



Response to Comments on the Quality of English Language

Point 1: There are some formatting and spelling errors, please check the whole manuscript carefully.

Response 1: As for formatting, the correct adjustment was made to all paragraphs. an indent of 4.6 centimeters to the left and 0.75 cm in the first paragraph. All subsections were also italicized, as requested by the Journal Fermentation

On page 3 line 136, it was changed from " in section" to " in the section".

On page 4 line 168, it was changed from " part for air inlet " to " part for an air ".

On page 4 line 172, a comma was placed after the word bioreactor.

On page 6 line 231, "at" was placed after the word observed and "The" before Hitachi.

On page 7 line 261, "to" was placed before the word chemical.

On page 8 line 288, it was changed from " by using LED-light assisted" to " by using an LED-light assisted".

On page 8 line 290, it was changed from " regime" to " regimes".

On page 8 line 305, it was changed from " observed increase " to " observed an increase ".

On page 12 line 404, it was changed from " highest values of emulsification index " to " highest values of the emulsification index ".

Author Response File: Author Response.pdf

Back to TopTop