Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato

Mancini, Lorenzo; Farinon, Barbara; Fumelli, Ludovica; Picarella, Maurizio Enea; Mazzucato, Andrea; Olivieri, Fabrizio

doi:10.3390/horticulturae11020129

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Open AccessFeature PaperArticle

Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato

by

Lorenzo Mancini

^†,

Barbara Farinon

^†

,

Ludovica Fumelli

,

Maurizio Enea Picarella

,

Andrea Mazzucato

^*

and

Fabrizio Olivieri

^*

Department of Agriculture and Forestry Science (DAFNE), Tuscia University, Via S Camillo de Lellis Snc, 01100 Viterbo, Italy

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Horticulturae 2025, 11(2), 129; https://doi.org/10.3390/horticulturae11020129

Submission received: 20 December 2024 / Revised: 17 January 2025 / Accepted: 22 January 2025 / Published: 25 January 2025

(This article belongs to the Special Issue Genomics and Genetic Diversity in Vegetable Crops)

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Abstract

The genetic diversity of tomato in Italy and the growing interest in high-quality food products highlight the importance of establishing varietal distinctiveness through molecular strategies to ensure agrifood product quality and traceability. In this study, four Italian potato-like leaf (PL) landraces were analyzed: “Spagnoletta di Formia e di Gaeta” (SPA) from southern Lazio, “Giagiù” (GIA) and “Patanara” (PTN) from Campania, and “Pomodoro di Mola” (MOL) from Apulia. These landraces were genotyped for the potato leaf gene (C), with two PL American genotypes and a non-allelic PL mutant line included as outgroups. Nagcarlang served as control. An allelism test confirmed C as determinant gene. The SCAR marker for C revealed that the Italian landraces presented determinants other than the most representative one responsible for PL. Whole-genome sequencing of SPA identified a private novel nonsense SNP variant allele, confirmed through dCAPS marker analysis. Additionally, two novel PL alleles responsible for missense variations were identified in GIA/PTN and MOL. In silico protein analysis suggested that novel C alleles could be functional determinants for the protein activity. Overall, PL mutations identified for the first time could serve as molecular tools for agrifood chain traceability, enabling early differentiation and recognition of genotypically similar varieties.

Keywords: Solanum lycopersicum L.; molecular markers; sequencing; qualitative traits; landraces; agri-food traceability

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MDPI and ACS Style

Mancini, L.; Farinon, B.; Fumelli, L.; Picarella, M.E.; Mazzucato, A.; Olivieri, F. Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato. Horticulturae 2025, 11, 129. https://doi.org/10.3390/horticulturae11020129

AMA Style

Mancini L, Farinon B, Fumelli L, Picarella ME, Mazzucato A, Olivieri F. Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato. Horticulturae. 2025; 11(2):129. https://doi.org/10.3390/horticulturae11020129

Chicago/Turabian Style

Mancini, Lorenzo, Barbara Farinon, Ludovica Fumelli, Maurizio Enea Picarella, Andrea Mazzucato, and Fabrizio Olivieri. 2025. "Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato" Horticulturae 11, no. 2: 129. https://doi.org/10.3390/horticulturae11020129

APA Style

Mancini, L., Farinon, B., Fumelli, L., Picarella, M. E., Mazzucato, A., & Olivieri, F. (2025). Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato. Horticulturae, 11(2), 129. https://doi.org/10.3390/horticulturae11020129

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Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato

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