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Journal of Mind and Medical Sciences is published by MDPI from Volume 12 Issue 1 (2025). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Valparaiso University (ValpoScholar).
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Article

Comparative Cytotoxicity Study of Nicotine and Cotinine on MRC-5 Cell Line

by
Ana-Maria Vlasceanu
1,
Daniela L. Baconi
1,*,
Bianca Galateanu
2,
Miriana Stan
1 and
Cristian Balalau
3
1
Department of Toxicology, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
2
Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania
3
St. Pantelimon Hospital, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
*
Author to whom correspondence should be addressed.
J. Mind Med. Sci. 2018, 5(1), 117-122; https://doi.org/10.22543/7674.51.P117122
Submission received: 25 January 2018 / Revised: 25 February 2018 / Accepted: 25 March 2018 / Published: 31 March 2018

Highlights

  • At concentrations similar to the levels observed in the plasma of smokers, nicotine and cotinine were not cytotoxic on MRC-5 cell line.
  • Higher concentrations (2mM) of both nicotine and cotinine decreased the viability of MRC-5 cells.
  • Nicotine proved to be more cytotoxic than cotinine. Unlike nicotine, the proliferative capacity of MRC-5 cells is maintained after 48 hours of cotinine treatment.

Abstract

Nicotine has several health hazards regarding carcinogenic potential. It also imparts increased risk for respiratory, cardiovascular, and gastrointestinal disorders. Several mechanisms have been proposed for the carcinogenic potential, including effects on cell proliferation, inducing oxidative stress, DNA mutation, or inhibition of apoptosis. The cotinine metabolite is generally thought to have effects similar to nicotine in some experimental systems. The purpose of this study was to assess the nicotine and cotinine cytotoxicity on MRC-5 lung fibroblasts. The pulmonary fibroblasts were treated with various concentrations of nicotine or cotinine (in the range 1 µM – 2 mM) for 24 or 48 h and analyzed for cell viability by MTT test. The results indicated that high nicotine concentrations (2 mM) induced marked cell death (about 50%) in MRC-5 cell line. Cotinine showed lower toxicity than nicotine on the MRC-5 cells. In contrast to nicotine treatment, cells treated with cotinine continued to proliferate after the 48h incubation period.
Keywords: nicotine; cotinine; cytotoxicity; MRC-5; MTT-assay nicotine; cotinine; cytotoxicity; MRC-5; MTT-assay

Share and Cite

MDPI and ACS Style

Vlasceanu, A.-M.; Baconi, D.L.; Galateanu, B.; Stan, M.; Balalau, C. Comparative Cytotoxicity Study of Nicotine and Cotinine on MRC-5 Cell Line. J. Mind Med. Sci. 2018, 5, 117-122. https://doi.org/10.22543/7674.51.P117122

AMA Style

Vlasceanu A-M, Baconi DL, Galateanu B, Stan M, Balalau C. Comparative Cytotoxicity Study of Nicotine and Cotinine on MRC-5 Cell Line. Journal of Mind and Medical Sciences. 2018; 5(1):117-122. https://doi.org/10.22543/7674.51.P117122

Chicago/Turabian Style

Vlasceanu, Ana-Maria, Daniela L. Baconi, Bianca Galateanu, Miriana Stan, and Cristian Balalau. 2018. "Comparative Cytotoxicity Study of Nicotine and Cotinine on MRC-5 Cell Line" Journal of Mind and Medical Sciences 5, no. 1: 117-122. https://doi.org/10.22543/7674.51.P117122

APA Style

Vlasceanu, A.-M., Baconi, D. L., Galateanu, B., Stan, M., & Balalau, C. (2018). Comparative Cytotoxicity Study of Nicotine and Cotinine on MRC-5 Cell Line. Journal of Mind and Medical Sciences, 5(1), 117-122. https://doi.org/10.22543/7674.51.P117122

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