Applied Sciences

2022

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14 pages, 2736 KiB

Open AccessArticle

Ultrasound-Assisted Extraction of β-Asarone from Sweet Flag (Acorus calamus) Rhizome

by Noridayu Omer, Yeun-Mun Choo, Muthupandian Ashokkumar and Nor Saadah Mohd Yusof

Appl. Sci. 2022, 12(21), 11007; https://doi.org/10.3390/app122111007 - 30 Oct 2022

Cited by 4 | Viewed by 2267

Abstract

In this study, the extraction efficiency of β-asarone from Malaysian Acorus calamus from Acoraceae family using conventional solvent extraction and ultrasound-assisted extraction techniques was compared. The results showed that the ultrasound-assisted extraction technique significantly improves the extraction yields and process feasibility without changing [...] Read more.

In this study, the extraction efficiency of β-asarone from Malaysian Acorus calamus from Acoraceae family using conventional solvent extraction and ultrasound-assisted extraction techniques was compared. The results showed that the ultrasound-assisted extraction technique significantly improves the extraction yields and process feasibility without changing the structure of the active compound, i.e., β-asarone. The extraction yield increment was found to be ~2.5-fold and ~1.6-fold at 1:100 and 1:50 solid-to-solvent ratio, at 30% applied sonication power. The positive impact of sonication can also be observed for both mechanistic stages of extraction, i.e., the washing and diffusion stages, due to the favorable physical effect of acoustic cavitations. The observation was supported by the SEM images of the plant residue. The characterization of the extract was carried out using HPLC, NMR, UV and IR techniques. In conclusion, ultrasound assistance increases the extraction efficiency by ~2.5-fold even at only 30% applied ultrasonic power at a 1:100 solid-to-solvent ratio. The present study also provides an efficient and simple method for accurate direct dosing of Acorus calamus extracts to an application. Full article

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16 pages, 312 KiB

Open AccessArticle

Influence of High-Power Ultrasound on Yield of Proteins and Specialized Plant Metabolites from Sugar Beet Leaves (Beta vulgaris subsp. vulgaris var. altissima)

by Josipa Dukić, Matea Hunić, Marinela Nutrizio and Anet Režek Jambrak

Appl. Sci. 2022, 12(18), 8949; https://doi.org/10.3390/app12188949 - 6 Sep 2022

Cited by 12 | Viewed by 2171

Abstract

Ultrasound with water as a green solvent is an effective strategy for reducing losses and increasing the utilization of by-products. The extraction of proteins and specialized plant metabolites from sugar beet leaves (Beta vulgaris subsp. vulgaris var. altissima) promotes sustainability in [...] Read more.

Ultrasound with water as a green solvent is an effective strategy for reducing losses and increasing the utilization of by-products. The extraction of proteins and specialized plant metabolites from sugar beet leaves (Beta vulgaris subsp. vulgaris var. altissima) promotes sustainability in the agro-food chain. Guided by sustainability, samples treated with ultrasound showed lower energy consumption and lower CO₂ emissions. Furthermore, the spectrophotometric determination revealed higher protein and phenol yields in ultrasonically treated samples compared to thermally treated ones. The highest yield of total proteins, 147.91 ± 4.58 mg (g_d.m.)⁻¹, was observed during ultrasound treatment (amplitude 100%, treatment time 9 min). Under the same extraction conditions, the same trend was observed in the yield of total phenols 17.89 ± 0.38 mg (g_d.m.)⁻¹. High-power ultrasound, compared to the thermal extraction method, has increased the yield of proteins and specialized plant metabolites with significantly lower energy consumption and CO₂ emissions. The obtained results are in accordance with the foundations of sustainable development. From an economic and environmental point of view, ultrasound with the use of green solvents would be an excellent replacement for conventional extraction methods. Full article

10 pages, 1305 KiB

Open AccessArticle

Effect of Ultrasonic Pulses on the Functional Properties of Stickwater

by Francisco Cadena-Cadena, Dulce Alondra Cuevas-Acuña, Felipe de Jesús Reynaga-Franco, Gilberto Rodríguez-Felix, María del Socorro Núñez-Ruiz, Odilia Azucena Higuera-Barraza, Alba Rocio Ochoa-Meza and Joe Luis Arias-Moscoso

Appl. Sci. 2022, 12(3), 1351; https://doi.org/10.3390/app12031351 - 27 Jan 2022

Cited by 1 | Viewed by 2107

Abstract

Large volumes of waste are generated in the processing operations of the fishing industry. These effluents contain potentially useful proteins. However, it is necessary to concentrate them for utilization. The stickwater (SW) resulting from this operation was subjected to a protein-fractionation step, pH [...] Read more.

Large volumes of waste are generated in the processing operations of the fishing industry. These effluents contain potentially useful proteins. However, it is necessary to concentrate them for utilization. The stickwater (SW) resulting from this operation was subjected to a protein-fractionation step, pH adjustment (acid + alkaline) and ultrasonic pulsing in order to aid in hydrolysis and evaluate its functional and nutritional properties. The protein fractions, as well as the protein hydrolysates present in the tail water, had a chemical composition of 54.85 ± 4.21 and 74.81 ± 3.89 protein (%), 0.8 ± 0.1 and 0.2 ± 0.015 fat (%), 7.21 ± 0.67% ash (%), respectively. The increase in low-molecular-weight peptides results in an increase in free-radical scavenging activity. However, the increase in ferric-reducing antioxidant power may be due to the HCl treatment performed by the company. An increase in the functional properties of the samples treated with ultrasonic pulses was observed. Therefore, the chemical, nutritional and functional characteristics of stickwater suggest its potential use as a food additive. Full article

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2021

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19 pages, 4472 KiB

Open AccessArticle

Sea Bass Side Streams Valorization Assisted by Ultrasound. LC-MS/MS-IT Determination of Mycotoxins and Evaluation of Protein Yield, Molecular Size Distribution and Antioxidant Recovery

by Fadila Al Khawli, Noelia Pallarés, Francisco J. Martí-Quijal, Emilia Ferrer and Francisco J. Barba

Appl. Sci. 2021, 11(5), 2160; https://doi.org/10.3390/app11052160 - 1 Mar 2021

Cited by 8 | Viewed by 2689

Abstract

Sea bass side streams obtained from the fish industry can be a good source of nutrients such as high-quality protein, lipids, and antioxidants. In this context, it is interesting to develop innovative approaches to extract the added-value compounds from fish side streams. In [...] Read more.

Sea bass side streams obtained from the fish industry can be a good source of nutrients such as high-quality protein, lipids, and antioxidants. In this context, it is interesting to develop innovative approaches to extract the added-value compounds from fish side streams. In this study, a strategy to obtain valuable compounds and to minimize the presence of toxins from fish side streams assisted by ultrasound technology is presented. For this purpose, ultrasound-assisted extraction (UAE) conditions have been optimized based on a response surface methodology (RSM) with the dependent variables: time (0.5–30 min), pH (5.5–8.5), and temperature (20–50 °C). After the treatment, protein extraction and antioxidant activity were evaluated in the extracts obtained from sea bass side streams using some spectrophotometric and fluorometric methods. Furthermore, mycotoxin presence was evaluated by LC-MS/MS-QTRAP. The results obtained revealed a high recovery percentage of proteins and antioxidant activity in the UAE extracts, especially those obtained from viscera, when the time and temperature increased to 30 min and 50 °C. Furthermore, none of the analyzed mycotoxins were detected in the sea bass side streams extracts under the studied variables. The experimental values obtained were close to the expected values, confirming the validity of the model employed to establish the optimal UAE conditions. Full article

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17 pages, 4275 KiB

Open AccessFeature PaperArticle

Ultrasound Extraction Mediated Recovery of Nutrients and Antioxidant Bioactive Compounds from Phaeodactylum tricornutum Microalgae

by Fadila Al Khawli, Francisco J. Martí-Quijal, Noelia Pallarés, Francisco J. Barba and Emilia Ferrer

Appl. Sci. 2021, 11(4), 1701; https://doi.org/10.3390/app11041701 - 14 Feb 2021

Cited by 34 | Viewed by 4374

Abstract

In recent years, a growing interest has been shown in the use of microalgae due to their interesting nutritional and bioactive profiles. Green innovative processing technologies such as ultrasound-assisted extraction (UAE) avoid the use of toxic solvents and high temperatures, being a sustainable [...] Read more.

In recent years, a growing interest has been shown in the use of microalgae due to their interesting nutritional and bioactive profiles. Green innovative processing technologies such as ultrasound-assisted extraction (UAE) avoid the use of toxic solvents and high temperatures, being a sustainable alternative in comparison with traditional extraction methods. The present study aims to evaluate the recovery of high added-value compounds from Phaedoactylum tricornutum assisted by ultrasound. To optimize the UAE of proteins, carbohydrates, pigments and antioxidant compounds, a response surface methodology was used. Carbohydrate extraction was positively affected by the temperature. However, for the extraction of carotenoids, the most influential factor was the extraction time. The total polyphenols were only significantly affected by the extraction time. Finally, the antioxidant capacity, measured by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was strongly modulated by the extraction time, while for the oxygen radical antioxidant capacity (ORAC) assay, the most important parameter was the temperature, followed by the extraction time. The optimal conditions for the maximum extraction of nutrients, bioactive compounds and antioxidant capacity were 30 min, 50 ºC and a pH of 8.5. Finally, it has been seen that with these conditions, the extraction of fucoxanthin is allowed, although no differences were found between an ultrasound-assisted extraction and a shaking extraction (control). Full article

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2020

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12 pages, 3163 KiB

Open AccessArticle

Modification of Olive Leaves’ Surface by Ultrasound Cavitation. Correlation with Polyphenol Extraction Enhancement

by Natacha Rombaut, Tony Chave, Sergey I. Nikitenko, Mohamed EI Maâtaoui, Anne Sylvie Fabiano-Tixier and Farid Chemat

Appl. Sci. 2021, 11(1), 232; https://doi.org/10.3390/app11010232 - 29 Dec 2020

Cited by 13 | Viewed by 2803

Abstract

We investigated the impact of ultrasound at 20 kHz on olive leaves to understand how acoustic cavitation could increase polyphenol extraction. Application of ultrasound to whole leaf from 5 to 60 min enabled us to increase extraction from 6.96 to 48.75 µg eq. [...] Read more.

We investigated the impact of ultrasound at 20 kHz on olive leaves to understand how acoustic cavitation could increase polyphenol extraction. Application of ultrasound to whole leaf from 5 to 60 min enabled us to increase extraction from 6.96 to 48.75 µg eq. oleuropein/mL of extract. These results were correlated with Environmental Scanning Electron Microscopy, allowing for leaf surface observation and optical microscopy of treated leaf cross sections to understand histochemical modifications. Our observations suggest that the effectiveness of ultrasound applied to extraction is highly dependent on plant structure and on how this material will react when subjected to acoustic cavitation. Ultrasound seems to impact the leaves by two mechanisms: cuticle erosion, and fragmentation of olive leaf surface protrusions (hairs), which are both polyphenol-rich structures. Full article

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16 pages, 3432 KiB

Open AccessArticle

A One-Pot Ultrasound-Assisted Almond Skin Separation/Polyphenols Extraction and its Effects on Structure, Polyphenols, Lipids, and Proteins Quality

by Malak Tabib, Yang Tao, Christian Ginies, Isabelle Bornard, Njara Rakotomanomana, Adnane Remmal and Farid Chemat

Appl. Sci. 2020, 10(10), 3628; https://doi.org/10.3390/app10103628 - 24 May 2020

Cited by 15 | Viewed by 4370

Abstract

Almond skin is an important by-product in the almond processing industry, rich in potentially health-promoting phenolic compounds. The objective of this present study is to separate the skin from the almond and extract its polyphenol contents using Ultrasound-Assisted Extraction (UAE) at room temperature. [...] Read more.

Almond skin is an important by-product in the almond processing industry, rich in potentially health-promoting phenolic compounds. The objective of this present study is to separate the skin from the almond and extract its polyphenol contents using Ultrasound-Assisted Extraction (UAE) at room temperature. Optimization was performed according to a two-variable central composite design (CCD), and the optimum combination of ultrasonic intensity and extraction temperature was obtained through multi-response optimization: ultrasonic intensity (UI), 9.47 W.cm⁻²; and temperature, 20 °C for an extraction time of 20 min. Under the above-mentioned conditions, total phenolic content was 258% higher with UAE than silent experiment. Mathematic modelling and microscopic investigations were achieved to enable understanding physical and structural effects of ultrasound on almond skins and comprehension of the mechanism behind the enhancement of mass transfer phenomena. Scanning Electron Microscopy (SEM) showed different acoustic cavitation impacts including fragmentation, sonoporation, and erosion. Extracts were analyzed by ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), identifying a combination of flavanols, flavanones and non-flavonoids. UAE shows no negative effect on almond proteins and lipids when compared to natural almonds (NS). Full article

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11 pages, 1006 KiB

Open AccessArticle

High-Intensity Ultrasound Pulses Effect on Physicochemical and Antioxidant Properties of Tilapia (Oreochromis niloticus) Skin Gelatin

by Dulce Alondra Cuevas-Acuña, Joe Luis Arias-Moscoso, Wilfrido Torres-Arreola, Francisco Cadena-Cadena, Ramón Gertrudis Valdez-Melchor, Sarai Chaparro-Hernandez, Hisila del Carmen Santacruz-Ortega and Saúl Ruiz-Cruz

Appl. Sci. 2020, 10(3), 1004; https://doi.org/10.3390/app10031004 - 3 Feb 2020

Cited by 8 | Viewed by 2800

Abstract

Ultrasonic pulses are considered green technology for the improvement of the functional properties of proteins. In this study, four high-intensity ultrasound pulse treatments (ultrasound-pulsed gelatin (UPG)-42, UPG-52, UPG-71, UPG-84, and non-pulsed control gelatin (CG)) were applied to tilapia (Oreochromis niloticus) skin [...] Read more.

Ultrasonic pulses are considered green technology for the improvement of the functional properties of proteins. In this study, four high-intensity ultrasound pulse treatments (ultrasound-pulsed gelatin (UPG)-42, UPG-52, UPG-71, UPG-84, and non-pulsed control gelatin (CG)) were applied to tilapia (Oreochromis niloticus) skin gelatin in order to study their effect on its physicochemical and antioxidant properties; a non-treated gelatin was used as a control. UPGs showed a significant increase in soluble protein and surface hydrophobicity compared to the control gelatin, and no significant difference was found in the electrophoretic profiles. The effects on the secondary structure were studied by circular dichroism and infrared spectra, and these showed that the random coil conformation was the main component in all treatments and the ultrasonic treatments only affected the α-helix and β-sheet proportion. Finally, the ABTS ((2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) and FRAP (ferric reducing ability) assays demonstrated that ultrasound treatments could improve the antioxidant activity of gelatins as free radical scavengers and electron donors. These results suggest that high-intensity ultrasound pulse technology is useful to improve fish gelatin antioxidant properties, which could be associated with secondary structure disruption. Full article

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13 pages, 1607 KiB

Open AccessArticle

Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

by Gregorio Iván Peredo Pozos, Mario Alberto Ruiz-López, Juan Francisco Zamora Nátera, Carlos Álvarez Moya, Lucia Barrientos Ramírez, Mónica Reynoso Silva, Ramón Rodríguez Macías, Pedro Macedonio García-López, Ricardo González Cruz, Eduardo Salcedo Pérez and J. Jesús Vargas Radillo

Appl. Sci. 2020, 10(2), 560; https://doi.org/10.3390/app10020560 - 12 Jan 2020

Cited by 40 | Viewed by 7600

Abstract

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols [...] Read more.

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes. Full article

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2019

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21 pages, 774 KiB

Open AccessReview

Cavitation Technology—The Future of Greener Extraction Method: A Review on the Extraction of Natural Products and Process Intensification Mechanism and Perspectives

by Debabrata Panda and Sivakumar Manickam

Appl. Sci. 2019, 9(4), 766; https://doi.org/10.3390/app9040766 - 22 Feb 2019

Cited by 136 | Viewed by 14236

Abstract

With growing consumer demand for natural products, greener extraction techniques are found to be potential alternatives especially for pharmaceutical, nutraceutical, and cosmetic manufacturing industries. Cavitation-based technology has drawn immense attention as a greener extraction method, following its rapid and effective extraction of numerous [...] Read more.

With growing consumer demand for natural products, greener extraction techniques are found to be potential alternatives especially for pharmaceutical, nutraceutical, and cosmetic manufacturing industries. Cavitation-based technology has drawn immense attention as a greener extraction method, following its rapid and effective extraction of numerous natural products compared to conventional techniques. The advantages of cavitation-based extraction (CE) are to eliminate the application of toxic solvents, reduction of extraction time and to achieve better extraction yield, as well as purity. The cavitational phenomena enhance the extraction efficiency via increased mass transfer rate between the substrate and solvent, following the cell wall rupture, due to the intense implosion of bubbles. This review includes a detailed overview of the ultrasound-assisted extraction (UAE), negative pressure cavitation (NPC) extraction, hydrodynamic cavitation extraction (HCE) and combined extractions techniques which have been implemented for the extraction of high-value-added compounds. A list of essential parameters necessary for the maximum possible extraction yield has been discussed. The optimization of parameters, such as ultrasonic power density, frequency, inlet pressure of HC, extraction temperature and the reactor configuration denote their significance for better efficiency. Furthermore, the advantages and drawbacks associated with extraction and future research directions have also been pointed out. Full article

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2018

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12 pages, 2300 KiB

Open AccessArticle

Enhancement of the Neuroprotective Effect of Fermented Spirulina maxima Associated with Antioxidant Activities by Ultrasonic Extraction

by Woon Yong Choi, Do Hyung Kang, Soo-Jin Heo and Hyeon Yong Lee

Appl. Sci. 2018, 8(12), 2469; https://doi.org/10.3390/app8122469 - 3 Dec 2018

Cited by 15 | Viewed by 3586

Abstract

This study is the first demonstration of the neuroprotective activity of the marine alga, Spirulina maxima, fermented by Lactobacillus plantarum HY-08, which was freshly isolated from fermented vegetables. The combined process of the fermentation with ultrasonic extraction at 40 kHz for 4 [...] Read more.

This study is the first demonstration of the neuroprotective activity of the marine alga, Spirulina maxima, fermented by Lactobacillus plantarum HY-08, which was freshly isolated from fermented vegetables. The combined process of the fermentation with ultrasonic extraction at 40 kHz for 4 hr greatly increased the amounts of β-carotene in the extract up to 1.62 mg/g, along with a high extraction yield of 18.26%, compared to 1.03 mg/g and 12.8% from ultrasonic extraction itself, and even much higher than 0.81 mg/g and 10.6% from conventional water extraction. It was also proved that the neuroprotective activities of the extract were enhanced possibly by higher contents of β-carotene in the extract, compared to those from water extraction or ultrasonic extraction itself. This extract was found to have strong antioxidant properties, with 49.01% 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, which resulted in 90.30% neuroprotective activity. Specifically, the fermented extract markedly enhanced the brain-derived neurotrophic factor (BDNF)/p-CREB signaling pathways, which can effectively prevent memory impairment in mice caused by oxidative stress. β-Carotene also increased the expression of p-CREB and BDNF, and a mechanism of their upregulation by β-carotene is first proposed in this study. It can be concluded that the strong neuroprotective effect of the extract was markedly influenced by the antioxidant activities, due to the synergistic effects of high amounts of β-carotene and other biologically active substances in the extract that can be obtained by lactic acid fermentation, combined with ultrasonic processing. Full article

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10 pages, 4791 KiB

Open AccessArticle

Enhanced Harnessing of the Graviola Bioactive Components Using a Neoteric Sonication Cum Microwave Coadjuvant Extraction Protocol

by Se Chul Chun, Shang Xiaomin, Vimala Anthonydhason, Hyejin Jung, Shimels Tilahun Belachew, Judy Gopal and Diby Paul

Appl. Sci. 2018, 8(2), 232; https://doi.org/10.3390/app8020232 - 2 Feb 2018

Cited by 3 | Viewed by 5694

Abstract

Graviola is one of the most accomplished natural anticancer therapists gaining popularity in recent times. Harnessing the full benefit from tapping all of its rich bioactive reservoirs is absolutely worthy and mandatory. It is in this regard that a well optimized extraction methodology [...] Read more.

Graviola is one of the most accomplished natural anticancer therapists gaining popularity in recent times. Harnessing the full benefit from tapping all of its rich bioactive reservoirs is absolutely worthy and mandatory. It is in this regard that a well optimized extraction methodology gains paramount importance. In case of Graviola, no sophistication in terms of extraction methods is reported. A neoteric sonication cum microwave combined extraction technology was introduced that maximized the extraction process and minimized (7 min) the extraction time. The extraction efficiency was validated based on the significant enrichment of bioactive ingredients in Graviola extracts following the sonication cum microwave combined protocol. Full article

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2017

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1045 KiB

Open AccessArticle

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

by Woon Yong Choi and Hyeon Yong Lee

Appl. Sci. 2018, 8(1), 26; https://doi.org/10.3390/app8010026 - 25 Dec 2017

Cited by 18 | Viewed by 5381

Abstract

Under the optimal ultrasonification extraction conditions of 20.52 kHz for the frequency, 32.59 °C for the temperature, and 4.91 h for the process time, 17.98 mg/g of chlorophyll a was obtained. It was much higher than 13.81 mg/g from conventional 70% ethanol extraction [...] Read more.

Under the optimal ultrasonification extraction conditions of 20.52 kHz for the frequency, 32.59 °C for the temperature, and 4.91 h for the process time, 17.98 mg/g of chlorophyll a was obtained. It was much higher than 13.81 mg/g from conventional 70% ethanol extraction and even higher than other data from Spirulina. This yield was close to the predicted value of 18.21 mg/g from the second-order polynomial model with a regression coefficient of 0.969. This model showed the greatest significance with the ultrasonic frequency and process time and the least significance with the temperature. The extracts also showed high α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activities as 69.38%, compared to 58.25% for the extracts from the 70% ethanol extraction. It was first shown that the optimal extraction was effective at enhancing the neuroprotective activities possibly due to the synergistic effects of higher amounts of chlorophyll a and other bioactive substances in the extract, revealing a 90% protection of the growth of mouse neuronal cells and a great reduction in Reactive Oxygen Species (ROS) production. Full article

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719 KiB

Open AccessArticle

Enhancement of Skin Anti-Inflammatory Activities of Eclipta prostrata L. from the Ultrasonic Extraction Process

by Hyeon Yong Lee

Appl. Sci. 2017, 7(12), 1227; https://doi.org/10.3390/app7121227 - 28 Nov 2017

Cited by 4 | Viewed by 5008

Abstract

This work first showed that the skin anti-inflammatory activities of Eclipta prostrata L. (E. prostrata L.)n be increased through an ultrasonic extraction process at a frequency of 120 kHz with 70% ethanol at 40 °C for 6 h. The extract from the [...] Read more.

This work first showed that the skin anti-inflammatory activities of Eclipta prostrata L. (E. prostrata L.)n be increased through an ultrasonic extraction process at a frequency of 120 kHz with 70% ethanol at 40 °C for 6 h. The extract from the ultrasound extraction (UE) contained 378.6 mg/100 g of wedelolactone (a major bioactive substance in E. prostrata L.), compared with the 172.9 mg/100 g from a conventional extraction process with 70% ethanol at 80 °C for 12 h (EE). The UE showed less than 10% of low cytotoxicity against normal fibroblast cells, and also exhibited much higher antioxidant activities than that from the EE when treating with 0.3 mg/mL of the extracts. It was proved that the high antioxidant activities of the UE were closely correlated with the enhancement of skin anti-inflammatory activities by effectively suppressing the production of tumor necrosis factor-alpha and interleukin-6 from macrophages. The production of prostaglandin E₂ from human skin cells was also greatly reduced, compared with the EE for all ranges of the treatments. These results strongly indicate that the various biological activities of E. prostrata L. can be improved by a simple ultrasound extraction process because of the high elution of bioactive substances even at a low temperature. Full article

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