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Article

An Efficient Synthesis of Novel Dispirooxindole Derivatives via One-Pot Three-Component 1,3-Dipolar Cycloaddition Reactions

by
Zhibin Huang
1,†,
Qian Zhao
2,†,
Gang Chen
3,
Huiyuan Wang
1,
Wei Lin
1,
Lexing Xu
4,
Hongtao Liu
4,
Juxian Wang
4,
Daqing Shi
1,* and
Yucheng Wang
4,*
1
Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
2
Tianjin ShuiGe Hospital, Tianjin 300120, China
3
Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory, Xinchang 312500, China
4
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2012, 17(11), 12704-12717; https://doi.org/10.3390/molecules171112704
Submission received: 4 September 2012 / Revised: 16 October 2012 / Accepted: 18 October 2012 / Published: 26 October 2012
(This article belongs to the Section Organic Chemistry)
Browse Figures
Versions Notes

Abstract

:
A series of novel dispirooxindoles have been synthesized through three-component 1,3-dipolar cycloaddition of azomethine ylides generated in situ by the decarboxylative condensation of isatin and an α-amino acid with the dipolarophile 5-benzylidene-1,3-dimethylpyrimidine-2,4,6-trione. This method has the advantages of mild reaction conditions, high atom economy, excellent yields, and high regio- and stereo-selectivity.

Graphical Abstract

1. Introduction

In recent decades, multicomponent reactions (MCRs) have emerged as a powerful synthetic strategy due to their efficiency, atom economy, high selectivity and convenience in the construction of multiple new bonds, which permit a rapid access to combinatorial libraries of complex organic molecules for efficient lead structure identification and optimization in drug discovery [1,2,3,4]. According to this method, the products are formed in a single step and diversity can be achieved simply by varying the reacting components.
1,3-Dipolar cycloaddition of azomethine ylides with olefinic and acetylenic dipolarophiles has gained significance as it proceeds with high regiochemical and stereochemical selectivity yielding pyrroline and pyrrolidine derivatives [5,6,7], which are prevalent in a variety of biologically active compounds [8] and are also inhibitors of many diseases such as diabetes [9], cancer [10] and viral infections [11]. Because of their remarkable biological activities, significant efforts have been devoted to the synthesis of their novel derivatives.
Among the various nitrogen-containing heterocycles, functionalized pyrrolidine, pyrrolizidine and oxindole alkaloids have become important synthetic targets as they constitute classes of compounds with significant biological activity [12]. The synthesis of spiro compounds has drawn considerable attention of chemists as have their highly pronounced biological properties [13,14]. The spirooxindole system as the core structure of many pharmacological agents and natural alkaloids [15,16,17,18], and has potent nonpeptide p53-MDM2 inhibitory activity [19]. Elacomine, spirotryprostatins A and B are some of the alkaloids containing spiropyrrolidinyloxindole ring systems. Some spiropyrrolidines are potential antileukaemic and anticonvulsant agents [20] and possess antiviral and local anaesthetic activities [21].
Barbituric acid has widely been used in the manufacture of plastics [22], textiles [23], polymers [24] and pharmaceuticals [25,26,27,28]. Barbiturates (derivatives of barbituric acid) like pentobarbital and phenobarbital were long used as anxiolytics and hypnotics. Spirobarbiturates are a class of compounds with interesting pharmacological and physiological activity [29,30,31]. We have recently reported the regio- and stereoselective synthesis of novel dispirooxindole derivatives via multicomponent reactions [32,33,34,35,36]. To expand our research program which aims to synthesize new spiro compounds and nitrogen heterocycles with biological activities, we report herein, the efficient synthesis of a series of novel dispirooxindole derivatives in excellent yields by the three-component 1,3-dipolar cycloaddition reaction of nonstabilized azomethine ylides generated in situ by the decarboxylative condensation of isatin and α-amino acids with 5-benzylidene-1,3-dimethylpyrimidine-2,4,6-trione using ethanol under reflux conditions.

2. Results and Discussion

In an effort to optimize this process, the three-component reaction of isatin (1), sarcosine (2), and the dipolarophile 5-(4-bromobenzylidene)-1,3-dimethylpyrimidine-2,4,6-trione (3a) was carried out in various solvents under reflux conditions as a simple model reaction in order to determine the best reaction solvent (Scheme 1). The results are summarized in Table 1. As can be seen from the data, the reaction could be efficiently carried out in solvents such as ethanol, methanol, acetonitrile, THF and 1,4-dioxane. In particular, the reaction using ethanol as the solvent resulted in higher yields and shorter reaction times than those using methanol, acetonitrile, THF and 1,4-dioxane. Thus, ethanol, which is a low cost bio-renewable product with low toxicity to human health and is relatively non-hazardous to the environment was chosen as the solvent for all further reactions (Table 1, entry 1) [37].
Molecules 17 12704 g003 550
Scheme 1. The model reaction.
Scheme 1. The model reaction.
Molecules 17 12704 g003
Table
Table 1. Optimization of solvent effect on the model reaction a.
Table 1. Optimization of solvent effect on the model reaction a.
EntrySolventTime (h)Yield b (%)
1Ethanol284
2Methanol256
3Acetonitrile375
4Tetrahydrofuran (THF)680
51,4-Dioxane860
a Reaction conditions: isatin (0.5 mmol), sarcosine (0.5 mmol) and 5-(4-bromobenzylidene)-1,3-dimethylpyrimidine-2,4,6-trione (0.5 mmol) in solvent (10 mL) at reflux temperature; b Yields of isolated products.
Using the optimized reaction conditions, various structurally diverse 5-benzylidene-1,3-dimethyl-pyrimidine-2,4,6-triones were investigated (Table 2). It was found that the aromatic rings bearing either electron-withdrawing or electron-donating functional groups were suitable for the reaction, while the cycloaddition reactions with dipolarophiles carrying electron-donating substituents required a longer times and the yield decreased (Table 2, entry 2).
Table
Table 2. Synthesis of dispirooxindole derivatives 4 via three-component reaction. Molecules 17 12704 i001
Table 2. Synthesis of dispirooxindole derivatives 4 via three-component reaction. Molecules 17 12704 i001
EntryProductArTime (h)Yield (%)
14a4-BrC6H4284
24b4-CH3C6H42.575
34c4-NO2C6H41.590
44d4-ClC6H4288
In order to establish the scope of this cycloaddition reaction, we extended the same protocol using istain (1), L-thioproline (5) and dipolarophiles 3 under the same reaction conditions to give a series of cycloadducts 6 in excellent yields (Table 3).
Table
Table 3. Synthesis of dispirooxindole derivatives 6 via three-component reactions. Molecules 17 12704 i002
Table 3. Synthesis of dispirooxindole derivatives 6 via three-component reactions. Molecules 17 12704 i002
EntryProductArTime (h)Yield (%)
16a4-BrC6H4184
26b4-CH3C6H4281
36c4-NO2C6H4187
46d4-ClC6H4183
56eC6H5182
66f2-NO2C6H41.582
76g3,4-Cl2C6H31.588
86hThiophen-2-yl386
With the use of Discrete Fourier Transformation (DFT) and the B3LYP/6-31G computer programme [38], a geometrical optimization of product 4a was obtained and is shown in Figure 1. From Figure 1, we found that the geometry A (trans) was more stable than geometry B (cis) (∆E = 10.98 kJ/mol).
Molecules 17 12704 g001 550
Figure 1. Optimized geometry of 4a.
Figure 1. Optimized geometry of 4a.
Molecules 17 12704 g001
To expand the scope of the current method, acenaphthenequinone (7) was examined as a replacement for isatin (1). The desired products 8 were obtained under the optimized conditions. The results are summarized in Table 4.
Table
Table 4. Synthesis of dispirooxindole derivatives 8 via three-component reaction. Molecules 17 12704 i003
Table 4. Synthesis of dispirooxindole derivatives 8 via three-component reaction. Molecules 17 12704 i003
EntryProductArTime (h)Yield (%)
18aC6H5182
28b3,4-Cl3C6H31.580
38c3,4-OCH2OC6H3378
The structures of the products were identified by IR, 1H-NMR, 13C-NMR and HRMS spectra. The structure and regiochemistry of the products were assigned on the basis of their spectroscopic analysis. For example, in the 1H-NMR spectrum of compound 4c, a sharp singlet at δ 2.13 due to the N-methyl protons was seen. The benzylic proton exhibited a doublet of doublets at δ 3.71 (J = 10.4 Hz and 8.0 Hz). The off resonance decoupled 13C-NMR spectrum added conclusive support. The 13C-NMR spectrum of 5c showed peaks at δ 81.17 and δ 67.26 for the two spirocarbons, respectively. The mass spectrum of 4c showed a molecular ion peak at m/z 486.1392 (M+Na). The X-ray crystallographic study of single of 8b (Figure 2) not only confirmed the structure deduced from NMR spectroscopic studies, but also determined the stereochemical outcome of the cycloaddition.
Molecules 17 12704 g002 550
Figure 2. X-Ray crystal structure of compound 8b.
Figure 2. X-Ray crystal structure of compound 8b.
Molecules 17 12704 g002
Although the detailed mechanism of the above reaction has not been elucidated yet, the formation of 4 can be explained by the mechanism proposed in Scheme 2. The reaction proceeds through the generation of azomethine ylide (dipole 7) via the condensation of isatin (1) with sarcosine (2) and decarboxylation. The dipolarophile 3 regioselectively reacts with azomethine ylides (dipole 7) in ethanol to give the desired products dispiro compounds 4 (Scheme 2, path A). The cycloaddition proceeds via an endo transition state [39,40,41]. The regioselectivity in the product formation can be explained by considering the secondary orbital interaction (SOI) [42,43] of the orbital of the carbonyl group of dipolarophile 3 with those of the ylide 7 as shown in Scheme 2. In this transition state, these orbital interactions are typically orthogonal in nature and occur between the oxygen atom of the carbonyl of the isatin and the carbon atom of the carbonyl of the dipolarophile 3. Accordingly, the observed regioisomer 4 via path A is more favorable because of the SOI which is not possible in path B.
Molecules 17 12704 g004 550
Scheme 2. Proposed reaction mechanism for the formation of compound 4.
Scheme 2. Proposed reaction mechanism for the formation of compound 4.
Molecules 17 12704 g004

3. Experimental

3.1. General

All reagents were purchased from commercial sources and used without further purification. Melting points are uncorrected. IR spectra were recorded on a Nicolet 6700 spectrometer in KBr with absorptions in cm−1. 1H-NMR spectra were determined on a Varian Inova-300/400 MHz spectrometer in DMSO-d6 solution. J values are in Hz. Chemical shifts are expressed in ppm downfield from internal standard TMS. HRMS data were obtained using Bruker micrOTOF-Q instrument or TOF-MS instrument. The starting compounds 3 were prepared according to the previously reported procedures [44,45].

3.2. General Procedure for the Synthesis of Dispirooxindoles 4, 6 and 8

A dry 50 mL flask was charged with isatin (1) or acenaphthenequinone (7) (0.5 mmol), sarcosine (2) or L-thioproline (5) (0.5 mmol), dipolarophile 3 (0.5 mmol) and ethanol (10 mL). The mixture was stirred at reflux temperature for 1–3 h. After completion of the reaction (monitored by TLC), the solvent was removed under vacuum. The solid was recrystallized from ethanol, and then dried at 80 °C for 4h under vacuum to give compounds 4, 6 or 8.
2,7,9-Trimethyl-4-(4-bromophenyl)-1-(spiro-3'-indolino)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (4a). White solid; m.p. 180–182 °C; IR (KBr, cm−1): 3313, 2939, 1735, 1679, 1618, 1468, 1420, 1374, 1070, 753; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.12 (s, 3H, CH3), 2.88–2.89 (m, 6H, 2 × CH3), 3.60 (m, 1H, CH2), 3.89 (t, J = 8.0 Hz, 1H, CH2), 5.18 (t, J = 8.8 Hz, 1H, CH), 6.78 (d, J = 7.6 Hz, 1H, ArH), 6.82 (d, J = 7.2 Hz, 1H, ArH), 6.96 (t, J = 6.4 Hz, 1H, ArH), 7.13–7.15 (m, 2H, ArH), 7.26 (t, J = 6.8 Hz, 1H, ArH), 7.41 (d, J = 8.4 Hz, 2H, ArH), 10.51 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 28.52, 29.67, 35.92, 41.98, 56.60, 67.26, 81.17, 110.42, 112.85, 120.15, 122.12, 123.59, 125.16, 131.02, 131.56, 137.96, 143.70, 150.33, 164.76, 166.92, 175.70; HRMS: calculated for C23H2179BrN4O4Na [M+Na]+: 519.0638, found: 519.0621.
2,7,9-Trimethyl-4-(4-methylphenyl)-1-(spiro-3'-indolino)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (4b). White solid; m.p. 190–191 °C; IR (KBr, cm−1): 3321, 2949, 1735, 1689, 1672, 1515, 1471, 1373, 752; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.13 (s, 3H, CH3), 2.23 (s, 3H, CH3), 2.90 (s, 6H, 2 × CH3), 3.56–3.60 (m, 1H, CH2), 3.91 (t, J = 9.2 Hz, 1H, CH2), 5.19 (t, J =9.2 Hz, 1H, CH), 6.76–6.78 (m, 1H, ArH), 6.82–6.84 (m, 1H, ArH), 6.96 (t, J = 7.2 Hz, 1H, ArH), 7.04 (s, 4H, ArH), 7.26 (t, J = 7.6 Hz, 1H, ArH), 10.49 (s, 1H, NH); 13C-NMR (100 MHz, DMSO-d6): δ (ppm) 21.17, 28.47, 29.61, 35.91, 42.26, 56.84, 67.33, 81.08, 110.36, 122.09, 123.73, 125.09, 128.43, 129.37, 131.63, 135.38, 135.93, 143.66, 150.33, 164.77, 167.02, 175.77; HRMS: calculated for C24H24N4O4[M]+: 432.1792, found: 432.1800.
2,7,9-Trimethyl-4-(4-nitrophenyl)-1-(spiro-3'-indolino)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (4c). White solid; m.p. 188–190 °C; IR (KBr, cm−1): 3350, 2926, 1730, 1684, 1619, 1599, 1520, 1469, 1379, 1348, 754; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.13 (s, 3H, CH3), 2.90 (s, 6H, 2 × CH3), 3.71 (dd, J1 = 8.0 Hz, J2 = 10.4 Hz, 1H, CH2), 3.93 (t, J = 8.0 Hz, 1H, CH2), 5.30 (t, J = 9.2 Hz, 1H, CH), 6.78–6.84 (m, 2H, ArH), 6.97 (t, J = 7.6 Hz, 1H, ArH), 7.28 (t, J = 8.0 Hz, 1H, ArH), 7.44 (d, J = 8.8 Hz, 2H, ArH), 8.08 (d, J = 8.8 Hz, 2H, ArH), 10.57 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 30.51, 31.670, 37.85, 44.31, 58.50, 69.16, 83.07, 112.41, 124.11, 125.34, 125.67, 127.20, 131.77, 133.77, 145.70, 148.45, 148.98, 152.31, 166.77, 168.80, 177.57; HRMS: calculated for C23H21N5O6Na [M+Na]+: 486.1384, found: 486.1392.
2,7,9-Trimethyl-4-(4-chlorophenyl)-1-(spiro-3'-indolino)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (4d). White solid; m.p. 240–242 °C; IR (KBr, cm−1): 3317, 2926, 1736, 1718, 1679, 1620, 1570, 1468, 1379, 758; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.89 (s, 3H, CH3), 2.90 (s, 3H, CH3), 3.08 (s, 3H, CH3), 3.61 (dd, J1 = 8.4 Hz, J2 = 10.0 Hz, 1H, CH2), 3.89 (t, J = 8.0 Hz, 1H, CH2), 5.20 (t, J = 9.2 Hz, 1H, CH), 6.78 (d, J = 8.0 Hz, 1H, ArH), 6.82–6.84 (m, 1H, ArH), 6.96 (t, J = 7.6 Hz, 1H, ArH), 7.19–7.21 (m, 1H, ArH), 7.29 (d, J = 8.4 Hz, 3H, ArH), 7.33–7.35 (m, 1H, ArH), 10.52 (s, 1H, NH); 13C-NMR (100 MHz, DMSO-d6): δ (ppm) 28.53, 29.67, 35.93, 41.97, 56.69, 67.34, 81.18, 110.42, 122.13, 123.62, 125.17, 128.66, 130.63, 131.63, 131.72, 137.55, 143.72, 150.53, 164.78, 166.94, 175.70; HRMS: calculated for C23H2135ClN4O4 [M]+: 452.1251, found: 452.1260.
1,3-Dimethyl-5'-(4-bromophenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6a). White solid; m.p. 194–196 °C; IR (KBr, cm−1): 3236, 2926, 1741, 1719, 1679, 1615, 1469, 1376, 749; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.94 (s, 3H, CH3), 3.05–3.08 (m, 1H, CH2), 3.28–3.33 (m, 2H, CH2), 3.40–3.42 (m, 4H, CH3 and CH), 3.79 (d, J = 10.4 Hz, CH), 4.29 (d, J = 10.0 Hz, 1H, CH2), 4.96–5.00 (m, 1H, CH2), 6.82 (d, J = 7.2 Hz, 1H, ArH), 6.98–7.02 (m, 1H, ArH), 7.29 (t, J = 7.2 Hz, 1H, ArH), 7.39–7.41 (m, 2H, ArH), 7.46–7.48 (m, 2H, ArH), 7.61 (d, J = 7.2 Hz, 1H, ArH), 10.84 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 33.93, 34.09, 42.24, 55.22, 58.79, 76.38, 76.57, 85.45, 115.22, 125.77, 126.48, 127.08, 135.10, 136.20, 136.30, 137.28, 140.40, 147.07, 155.71, 169.89, 171.56, 180.61; HRMS: calculated for C24H2279BrN4O4S [M+H]+: 541.0540, found: 541.0559.
1,3-Dimethyl-5'-(4-methylphenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6b). White solid; m.p. 188–190 °C; IR (KBr, cm−1): 3213, 2921, 1740, 1684, 1620, 1472, 1369, 752; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.24 (s, 3H, CH3), 2.52 (s, 3H, CH3), 2.95 (s, 3H, CH3), 3.02 (dd, J1 = 3.2 Hz, J2 = 11.2 Hz, 1H, CH2), 3.31–3.33 (m, 1H, CH2), 3.38–3.40 (m, 1H, CH), 3.78–3.81 (m, 1H, CH), 4.28–4.31 (m, 1H, CH2), 4.98–5.03 (m, 1H, CH2), 6.82 (d, J = 7.6 Hz, 1H, ArH), 7.00 (t, J = 7.6 Hz, 1H, ArH), 7.07–7.09 (m, 2H, ArH), 7.27–7.32 (m, 3H, ArH), 7.61 (d, J = 7.6 Hz, 1H, ArH), 10.76 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 25.95, 33.90, 34.10, 42.45, 55.63, 58.78, 76.57, 76.63, 85.40, 115.26, 126.45, 127.13, 134.11, 134.72, 135.12, 136.13, 137.83, 141.50, 147.05, 155.68, 169.81, 171.56, 180.65; HRMS: calculated for C25H25N4 O4S [M]+: 477.1591, found: 477.1607.
1,3-Dimethyl-5'-(4-nitrophenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6c). White solid; m.p. 186–188 °C; IR (KBr, cm−1): 3205, 3086, 2952, 1741, 1683, 1522, 1419, 1348, 749; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.94 (s, 3H, CH3), 3.14–3.17 (m, 2H, CH2), 3.41 (s, 4H, CH3 and CH), 3.79 (d, J = 10.0 Hz, 1H, CH), 4.46–4.48 (m, 1H, CH2), 5.02 (s, 1H, CH2), 6.82–6.84 (m, 1H, ArH), 7.00–7.02 (m, 1H, ArH), 7.28–7.30 (m, 1H, ArH), 7.61–7.62 (m, 1H, ArH), 7.68–7.70 (m, 2H, ArH), 8.11–8.13 (m, 2H, ArH), 10.86 (s, 1H, NH); 13C-NMR (100 MHz, DMSO-d6): δ (ppm) 29.21, 29.39, 37.49, 50.57, 53.77, 71.59, 72.13, 80.60, 121.79, 122.27, 123.58, 130.29, 131.38, 131.53, 142.33, 144.55, 146.96, 150.95, 165.20, 166.83, 175.71; HRMS: calculated for C24H22N5O6S [M+H]+: 508.1285, found: 508.1290.
1,3-Dimethyl-5'-(4-chlorophenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6d). White solid; m.p. 162–164 °C; IR (KBr, cm−1): 3289, 3062, 2908, 1751, 1733, 1680, 1496, 1376, 755; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.87 (s, 3H, CH3), 2.89 (s, 3H, CH3), 3.18 (t, J = 9.6 Hz, 1H, CH2), 3.25–3.29 (m, 1H, CH2), 3.42 (d, J = 7.6 Hz, 1H, CH), 3.72 (d, J = 7.6 Hz, 1H, CH), 4.70–4.74 (m, 1H, CH2), 4.83–4.85 (m, 1H, CH2), 6.77–6.79 (m, 1H, ArH), 6.94–7.01 (m, 2H, ArH), 7.25–7.29 (m, 1H, ArH), 7.32–7.34 (m, 4H, ArH), 10.71 (s, 1H, NH); HRMS: calculated for C24H2135ClN4O4SNa [M+Na]+: 519.0864, found: 519.0871.
1,3-Dimethyl-5'-phenyl-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6e). White solid; m.p. 183–184 °C; IR (KBr, cm−1): 3212, 3082, 2953, 1740, 1680, 1615, 1472, 1367, 754; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.53 (s, 3H, CH3), 2.96 (s, 3H, CH3), 3.07 (dd, J1 = 3.6 Hz, J2 = 11.2 Hz, 1H, CH2), 3.33–3.35 (m, 1H, CH2), 3.39 (d, J = 10.0 Hz, 1H, CH), 3.80 (d, J = 10.4 Hz, 1H, CH), 4.36 (d, J = 10.0 Hz, 1H, CH2), 4.99–5.04 (m, 1H, CH2), 6.83 (d, J = 7.6 Hz, 1H, ArH), 7.01 (t, J = 7.6 Hz, 1H, ArH), 7.19–7.23 (m, 1H, ArH), 7.26–7.31 (m, 3H, ArH), 7.40–7.42 (m, 2H, ArH), 7.61 (d, J = 7.6 Hz, 1H, ArH), 10.77 (s, 1H, NH); 13C-NMR (100 MHz, DMSO-d6): δ (ppm) 34.27, 34.46, 42.90, 56.16, 59.00, 76.97, 85.73, 115.54, 126.83, 127.51, 132.64, 133.88, 135.04, 135.50, 136.49, 141.47, 147.38, 156.06, 170.16, 171.94, 180.97; HRMS: calculated for C24H23N4O4S [M+H]+: 463.1435, found: 463.1443.
1,3-Dimethyl-5'-(2-nitrophenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6f). White solid; m.p. 184–186 °C; IR (KBr, cm−1): 3220, 2947, 1743, 1685, 1616, 1536, 1471, 1370, 782, 763; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.34 (s, 3H, CH3), 2.97 (s, 3H, CH3), 3.00 (s, 1H, CH2), 3.04 (s, 1H, CH2), 3.10–3.14 (m, 1H, CH), 3.82 (d, J =10.8 Hz, 1H, CH), 4.88–4.90 (m, 1H, CH2), 5.07–5.11 (m, 1H, CH2), 6.81–6.83 (m, 1H, ArH), 7.01 (t, J = 7.6 Hz, 1H, ArH), 7.29 (t, J =7.6 Hz, 1H, ArH), 7.50 (t, J =8.0 Hz, 1H, ArH), 7.59 (d, J = 7.6 Hz, 1H, ArH), 7.68 (t, J = 8.0 Hz, 1H, ArH), 7.78 (d, J = 7.6 Hz, 1H, ArH), 8.36 (d, J = 8.0 Hz, 1H, ArH), 10.84 (s, 1H, NH); HRMS: calculated for C24H22N5O6S [M+H]+: 508.1285, found: 508.1296.
1,3-Dimethyl-5'-(3,4-dichlorophenyl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6g). White solid; m.p. 192–194 °C; IR (KBr, cm−1): 3074, 2957, 1757, 1721, 1688, 1614, 1469, 1367, 748; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.46 (s, 3H, CH3), 2.94 (s, 3H, CH3), 3.03 (dd, J1 = 2.4 Hz, J2 = 11.2 Hz, 1H, CH2), 3.23–3.28 (m, 1H, CH2), 3.40 (s, 1H, CH), 3.78 (d, J = 10.8 Hz, 1H, CH), 4.27 (d, J = 10.0 Hz, 1H, CH2), 4.99–5.04 (m, 1H, CH2), 6.83 (d, J = 7.6 Hz, 1H, ArH), 7.00 (t, J = 7.6 Hz, 1H, ArH), 7.30 (t, J = 7.6 Hz, 1H, ArH), 7.49–7.56 (m, 2H, ArH), 7.63 (d, J = 7.6 Hz, 1H, ArH), 7.71 (s, 1H, ArH), 10.84 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 28.69, 35.78, 43.32, 57.18, 67.90, 84.56, 120.35, 122.04, 122.14, 127.39, 128.96, 129.47, 130.19, 130.47, 130.92, 131.70, 132.97, 133.13, 137.67, 142.05, 149.92, 164.77, 167.07, 203.16; HRMS: calculated for C24H2135Cl2N4O4S [M+H]+: 531.0655, found: 531.0674.
1,3-Dimethyl-5'-(thiophen-2-yl)-7'-(spiro-3''-indolino)tetrahydro-1H,1'H-spiro[pyrimidine-5,6'-pyrrolo[1,2-c]thiazole]-2,4,6-trione (6h). White solid; m.p. 164–166 °C; IR (KBr, cm−1): 3243, 3078, 2956, 1738, 1679, 1568, 1439, 1382; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.88 (s, 3H, CH3), 2.92 (s, 3H, CH3), 3.12–3.17 (m, 2H, CH2), 3.44 (d, J = 7.6 Hz, 1H, CH), 3.70 (d, J = 7.2 Hz, 1H, CH), 4.72–4.76 (m, 1H, CH2), 5.05 (d, J = 8.4 Hz, 1H, CH2), 6.79 (d, J = 7.6 Hz, 1H, ArH), 6.92–7.00 (m, 4H, ArH), 7.25–7.29 (m, 1H, ArH), 7.37–7.38 (m, 1H, ArH), 10.74 (s, 1H, NH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 28.68, 29.70, 36.56, 43.83, 51.33, 73.06, 74.23, 79.44, 110.74, 122.54, 124.32, 125.06, 126.02, 127.49, 127.69, 132.03, 138.48, 142.86, 150.04, 164.75, 165.76, 176.46; HRMS: calculated for C22H21N4O4S2 [M+H]+: 469.0999, found: 469.0980.
2,7,9-Trimethyl-4-phenyl-1-(spiro-2'-acenaphthylenone)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (8a). White solid; m.p. 168–170 °C; IR (KBr, cm−1): 3063, 2920, 1748, 1723, 1685, 1442, 1371, 833, 783, 751; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.19 (s, 6H, 2 × CH3), 2.91 (s, 3H, CH3), 3.83 (t, J = 8.0 Hz, 1H, CH2), 4.08 (t, J = 8.0 Hz, 1H, CH2), 5.18 (t, J = 8.0 Hz, 1H, CH), 7.16 (s, 3H, ArH), 7.25 (s, 2H, ArH), 7.30 (d, J = 8.0 Hz, 1H, ArH), 7.72 (s, 1H, ArH), 7.82–7.88 (m, 2H, ArH), 8.04–8.06 (m, 1H, ArH), 8.29–8.31 (m, 1H, ArH); 13C-NMR (100 MHz, DMSO-d6): δ (ppm) 33.40, 33.45, 40.51, 48.79, 62.13, 72.64, 89.12, 126.78, 126.80, 131.85, 132.07, 133.12, 133.68, 133.70, 134.19, 135.06, 135.22, 137.60, 138.01, 143.03, 146.74, 154.67, 169.55, 171.95, 207.93; HRMS: calculated for C27H23N3O4Na [M+Na]+: 476.1581, found: 476.1582.
2,7,9-Trimethyl-4-(3,4-dichlorophenyl)-1-(spiro-2'-acenaphthylenone)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (8b). White solid; m.p. 192–194 °C; IR (KBr, cm−1): 2965, 1721, 1684, 1640, 1372, 783, 750; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.12 (s, 3H, CH3), 2.17 (s, 3H, CH3), 2.89 (s, 3H, CH3), 3.79 (t, J = 8.0 Hz, 1H, CH2), 4.05 (t, J = 8.0 Hz, 1H, CH2), 5.15 (t, J = 7.6 Hz, 1H, CH), 7.17–7.19 (m, 1H, ArH), 7.28–7.30 (m, 1H, ArH), 7.46–7.51 (m, 2H, ArH), 7.71–7.75 (m, 1H, ArH), 7.83–7.90 (m, 2H, ArH), 8.07 (d, J = 8.0 Hz, 1H, ArH), 8.32 (d, J = 7.2 Hz, 1H, ArH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 28.66, 35.73, 42.82, 56.93, 56.95, 68.00, 84.70, 120.77, 122.08, 122.15, 127.42, 128.89, 129.44, 129.99, 130.07, 130.42, 130.70, 131.09, 131.22, 131.39, 133.01, 139.12, 142.07, 149.89, 164.73, 166.92, 203.09; HRMS: calculated for C27H21Cl2N3O4Na [M+Na]+: 509.1113, found: 509.1338.
2,7,9-Trimethyl-4-(3,4-methylenedioxylphenyl)-1-(spiro-2'-acenaphthylenone)-2,7,9-triazaspiro[4.5]decane-6,8,10-trione (8c). White solid; m.p. 186–188 °C; IR (KBr, cm−1): 2939, 2897, 1730, 1692, 1500, 1364, 1233, 832, 783; 1H-NMR (400 MHz, DMSO-d6): δ (ppm) 2.16 (s, 6H, 2 × CH3), 2.91 (s, 3H, CH3), 3.73 (s, 1H, CH2), 4.02 (s, 1H, CH2), 5.10 (s, 1H, CH), 5.96 (s, 2H, CH2), 6.64 (s, 1H, ArH), 6.77–6.82 (m, 2H, ArH), 7.30 (s, 1H, ArH), 7.72–7.87 (m, 3H, ArH), 8.05 (s, 1H, ArH), 8.30 (s, 1H, ArH); 13C-NMR (75 MHz, DMSO-d6): δ (ppm) 27.39, 34.47, 42.72, 56.19, 67.02, 83.22, 100.23, 107.34, 108.13, 120.73, 120.89, 126.02, 127.68, 128.15, 129.06, 129.20, 130.11, 131.55, 132.06, 140.67, 145.25, 146.47, 148.62, 163.47, 165.86, 201.87; HRMS: calculated for C28H23N3O6 [M]+: 497.1587, found: 497.1585.

3.3. X-ray Crystallography [46]

The single-crystals of compound 8b were obtained by slow evaporation from ethanol. Intensity data were collected on a Bruker P4 diffractometer with graphite monochromated Mo Kα radiation (λ = 0.71073 Å) using the ω scan mode with 1.34º < θ < 25.02º; 4188 unique reflections were measured and 3254 reflections with I > 2σ(I) were used in the Fourier techniques. The final refinement was converged to R = 0.0428 and wR = 0.1266. Crystal data for 8b: empirical formula C27H21Cl2N3O4, crystal dimension 0.42 × 0.40 × 0.37 mm, triclinic, space group P-1, a = 8.0847(7) Å, b = 10.0554(10) Å, c = 15.5500(13) Å, α = 76.8210(10)º, β = 86.626(2)º, γ = 76.2960(10)º, V = 1195.79(19)Å3, Mr = 522.37, Z = 2, Dc = 1.451 Mg/m3, μ(Mo Kα) = 0.312 mm−1, F(000) = 540, S = 1.079.

4. Conclusions

In summary, we have successfully developed a 1,3-dipolar cycloaddition of azomethine ylides, and a series of novel dispiro cycloadducts were obtained. This method has the advantages of convenient operation, mild reaction conditions, short reaction time, and high efficiency.

Acknowledgments

We gratefully acknowledge the Natural Science Foundation of China (No. 81072577, 81102376, 21072144), the Major Basic Research Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions (No. 10KJA150049), the Priority Academic Project Development of Jiangsu Higher Education Institutions and Key Laboratory of Organic Synthesis of Jiangsu Province (No. KJS0812) and the National S&T Major Special Project on Major New Drug Innovation (No. 2012ZX093101002-001-017) for support of this research.

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  46. Crystallographic data for 8b have been deposited at the Cambridge Crystallographic Data Centre with the deposition number CCDC906792. Copies of these data can be obtained free of charge via www.ccdc.cam.ac.uk/conts/retrieving.html (or from the Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge, CB 2 1EZ, UK; Fax: +44-1223-336-033; or Email: [email protected]).
  • Sample Availability: Samples of the compounds 4 and 6 are available from the authors.

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Huang, Z.; Zhao, Q.; Chen, G.; Wang, H.; Lin, W.; Xu, L.; Liu, H.; Wang, J.; Shi, D.; Wang, Y. An Efficient Synthesis of Novel Dispirooxindole Derivatives via One-Pot Three-Component 1,3-Dipolar Cycloaddition Reactions. Molecules 2012, 17, 12704-12717. https://doi.org/10.3390/molecules171112704

AMA Style

Huang Z, Zhao Q, Chen G, Wang H, Lin W, Xu L, Liu H, Wang J, Shi D, Wang Y. An Efficient Synthesis of Novel Dispirooxindole Derivatives via One-Pot Three-Component 1,3-Dipolar Cycloaddition Reactions. Molecules. 2012; 17(11):12704-12717. https://doi.org/10.3390/molecules171112704

Chicago/Turabian Style

Huang, Zhibin, Qian Zhao, Gang Chen, Huiyuan Wang, Wei Lin, Lexing Xu, Hongtao Liu, Juxian Wang, Daqing Shi, and Yucheng Wang. 2012. "An Efficient Synthesis of Novel Dispirooxindole Derivatives via One-Pot Three-Component 1,3-Dipolar Cycloaddition Reactions" Molecules 17, no. 11: 12704-12717. https://doi.org/10.3390/molecules171112704

APA Style

Huang, Z., Zhao, Q., Chen, G., Wang, H., Lin, W., Xu, L., Liu, H., Wang, J., Shi, D., & Wang, Y. (2012). An Efficient Synthesis of Novel Dispirooxindole Derivatives via One-Pot Three-Component 1,3-Dipolar Cycloaddition Reactions. Molecules, 17(11), 12704-12717. https://doi.org/10.3390/molecules171112704

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