Dianthiamides A–E, Proline-Containing Orbitides from Dianthus chinensis

Abstract

Orbitides are plant-derived small cyclic peptides with a wide range of biological activities. Phytochemical investigation of the whole plants of Dianthus chinensis was performed with the aim to discover new bioactive orbitides. Five undescribed proline-containing orbitides, dianthiamides A–E (1–5), were isolated from a methanolic extract of Dianthus chinensis. Their structures were elucidated by extensive analysis of 1D and 2D NMR and HRESI–TOF–MS as well as ESI–MS/MS fragmentation data. The absolute configuration of the amino acid residues of compounds 1–5 was determined by Marfey’s method. All compounds were tested for their cytotoxic activity, and dianthiamide A (1) exhibited weak activity against A549 cell line with IC₅₀ value of 47.9 μM.

1. Introduction

Dianthus chinensis L. is a perennial herbaceous plant belonging to the Caryophyllaceae family, and is distributed widely in Europe and Eastern Asia. The whole plant of D. chinensis is commonly used as a traditional medicine in Korea for treating diuretic, carcinoma, urethritis, and carbuncles [1,2,3,4,5,6]. Previous investigation on the phytochemical constituents of the genus Dianthus led to the isolation of cyclopeptides [4,5,6,7,8,9], dianthramides [5,10,11], triterpenoidal saponins [1,2,3,12,13], anthocyanins [14], and pyran-type glycosides [15]. Orbitides, formerly known as Caryophyllaceae-type cyclic peptides, are N-to-C cyclized plant peptides lacking disulfide bonds, which possess 5 to 12 amino acid residues. Orbitides are ribosomally synthesized and post-translationally modified cyclic peptides, which have been discovered in many plants of the families such as Annonaceae, Asteraceae, Caryophyllaceae, Euphorbiaceae, Lamiaceae, Linaceae, Phytolaccaceae, Rutaceae, Schizandraceae, and Verbenaceae. Recently, orbitides have gained increasing attention owed to a wide range of biological activities including cytotoxic, antimalarial, immunomodulatory, and antiproliferative activities [16,17,18,19]. The Dianthus genus is a rich source of proline-containing orbitides, some of which showed cytotoxic activity against several cancer cell lines [5,6,18,20]. Therefore, we have embarked on a research program for the isolation of new bioactive orbitides from medicinal plant, and five undescribed orbitides, dianthiamides A–E (1–5) (Figure 1), were isolated from a MeOH extract of the whole plants of the D. chinensis. Herein, the isolation and structure determination as well as their cytotoxic activity against A549 cell line are described.

2. Results and Discussion

Dianthiamide A (1) was obtained as a yellow amorphous powder. Its molecular formula of C₃₇H₅₄N₈O₈ was determined from the HRESI–TOF–MS data (m/z 739.4144 [M + H]⁺; calcd for 739.4137). The ¹H and ¹³C NMR in conjunction with HSQC data of 1 displayed the presence of 37 carbon signals assigned to eight amide carbonyl carbons (δ_C 168.8, 169.8, 170.8, 170.9, 171.1, 171.4, 172.3, and 173.4), seven α-amino acid carbons [δ_C 44.4 (CH₂), 48.6 (CH), 49.6 (CH), 56.0 (CH), 59.6 (CH), 60.8 (CH), and 61.6 (CH)], six aromatic carbons [δ_C 126.8, 128.8, (2C), 129.1 (2C), and 138.4], two methines, ten methylenes, and four methyls (

Table 1. ¹H and ¹³C NMR data for dianthiamide A (1) (DMSO-d₆, 700 MHz, d in ppm, J in Hz).

Position	δC	δH (J in Hz)	Position	δC	δH (J in Hz)
Gly1			cis-Prob5
C=O	169.8, C		C=O	170.9, C
NH		8.70 (t, 4.2)	α	60.8, CH	4.55 (d, 7.0)
α	44.4, CH2	3.55 (dd, 16.8, 4.2)	β	31.1, CH2	2.33 (dd, 11.9, 7.0)
		3.40 (dd, 16.8, 5.6)			1.92 (m)
Phe2			γ	22.1, CH2	1.85 (m)
C=O	171.1, C				1.46 (m)
NH		7.69 (d, 9.1)	δ	46.4, CH2	3.46 (m)
α	56.0, CH	4.38 (m)			3.25 (t, 9.8)
β	37.0, CH2	3.01 (m)	Ile6
		2.97 (m)	C=O	171.4, C
Phe-1′	138.4, C		NH		8.49 (d, 7.7)
2′,6′	129.1, CH	7.15–7.27 (m)	α	61.6, CH	3.66 (dd, 9.8, 7.7)
3′,5′	128.8, CH	7.15–7.27 (m)	β	35.3, CH	2.05 (m)
4′	126.8, CH	7.15–7.27 (m)	γ-CH3	16.0, CH3	0.90 (t, 7.7)
Leu3
C=O	168.8, C		γ-CH2	25.9, CH2	1.50 (m)
NH		7.28 (m)			1.15 (m)
α	48.6, CH	4.48 (m)	δ-CH3	10.9, CH3	0.82 (d, 7.7)
β	41.0, CH2	1.68 (m)	Asn7
		1.19 (m)	C=O	172.3, C
γ-CH	24.6, CH	1.51 (m)	NH		7.26 (m)
δ-CH3	23.6, CH3	0.85 (d, 6.3)	α	49.6, CH	4.18 (m)
δ-CH3	22.6, CH3	0.88 (d, 6.3)	β	35.8, CH2	3.15 (m)
trans-Proa4					3.05 (m)
C=O	170.8, C		C=O	173.4, C
α	59.6, CH	4.45 (t, 7.0)	NH2		7.97 (s)
β	28.5, CH2	2.27 (m)			7.54 (s)
		1.64 (m)
γ	25.3, CH2	1.96 (m)
		1.83 (m)
δ	47.1, CH2	3.44 (m)

), suggesting that 1 is a heptapeptide. Furthermore, HSQC, HMBC, and COSY spectra showed the identification of seven amino acid residues including phenylalanine (F), glycine (G), isoleucine (I), asparagine (N), leucine (L), and two prolines (P_a and P_b).

In the HMBC and ROESY experiments, the cyclic feature and amino acid sequence of 1 were elucidated by the correlations observed between the amino acid Hα and continuous amide group (CONH). Therefore, the linear sequence of 1 was identified as G-F-L-P_a-P_b-I-N. Also, the HMBC correlation from Gly-Hα (δ_H 3.40 and 3.55) to Asn-C=O (δ_C 172.3) as well as the ROESY correlation between Gly-NH (δ_H 8.70) and Asn-Hα (δ_H 4.18) established the cyclic heptapeptide as cyclo-G-F-L-P_a-P_b-I-N (Figure 2). The amino acid sequence of 1 was further confirmed by analysis of the ESI–MS/MS fragment ions. Presumably, though there were several ring-opening sites, it occurred at two preferred positions at Pro_a⁴-Pro_b⁵ and Ile⁶-Asn⁷, respectively. Each the linear sequences, Pro_b⁵-Ile⁶-Asn⁷-Gly¹-Phe²-Leu³-Pro_a⁴ (b₇P_bP_a) and Asn⁷-Gly¹-Phe²-Leu³-Pro_a⁴-Pro_b⁵-Ile⁶ (b₇NI) was certified by acylium ions (b_nP_bP_a and b_nNI) and after loss of CO (a_nNI) at m/z 642 (b₆P_bP_a), 626 (b₆NI), 529 (b₅P_bP_a and b₅NI), 404 (a₄NI), 364 (b₄P_bP_a-H₂O), 319 (b₃NI), and 211 (b₂P_bP_a), corresponding to the successive loss of amino acid residues (Figure S9).

The geometry of proline residues was assigned on the basis of the Δδ_Cβ-Cγ values and the presence of a ROE correlations between the proline Hα or Hδ and the Hα of previous amino acid. The Δδ_Cβ-Cγ value (3.2 ppm) of the Pro_a⁴ and the ROE correlation between the Hα (δ_H 4.48) of Leu³ and the Hδ (δ_H 3.44) of Pro_a⁴ indicated that the amide bond in the Pro_a⁴ was a trans. However, the Δδ_Cβ-Cγ value (9.0 ppm) of the Pro_b⁵ and the ROE correlation between the Hα (δ_H 4.45) of Pro_a⁴ and the Hα (δ_H 4.55) of Pro_b⁵ indicated that the geometry of Pro_b⁵ was a cis (Figure 3) [21,22,23]. The absolute configuration of amino acid residues in 1 were identified as L configuration, which was deduced by acid hydrolysis and Marfey’s derivatization, followed by HPLC analysis [24,25,26,27]. The N-α-(2,4-dinitro-5-fluorophenyl)-l-alaninamide (l-FDAA)-derivatives of 1 gave peaks at t_R (min) 15.0 (l-Asp, m/z 386), 20.8 (l-Pro, m/z 368), 29.5 (l-Leu, m/z 384), 29.7 (l-Phe, m/z 418), and 30.2 (l-Ile, m/z 384) (Figure S10). Therefore, dianthiamide A (1) was established as cyclo-(Gly¹-l-Phe²-l-Leu³-l-trans-Pro_a⁴-l-cis-Pro_b⁵-l-Ile⁶-l-Asn⁷).

Dianthiamide B (2) was isolated as a yellow amorphous powder, the HRESI–TOF–MS data were consistent with the molecular formula C₃₂H₄₄N₆O₇ (m/z 647.3161 [M + Na]⁺; calcd for 647.3163). The ¹H, ¹³C and HSQC NMR spectra of 2 showed 32 carbons, consisting of seven amide carbonyl carbons, six α-amino acid carbons, six aromatic carbons, two methines, seven methylenes, and four methyls (

Table 2. ¹H and ¹³C NMR data for dianthiamide B (2) (DMSO-d₆, 700 MHz, d in ppm, J in Hz).

Position	δC	δH (J in Hz)	Position	δC	δH (J in Hz)
Gly1			Phe4
C=O	168.7, C		C=O	170.7, C
NH		8.68 (m)	NH		8.68 (m)
α	43.3, CH2	3.65 (t, 7.0)	α	57.2, CH	3.80 (m)
Leu2			β	33.8, CH2	3.33 (m)
C=O	170.7, C				3.30 (m)
NH		6.50 (m)	Phe-1′	139.6, C
α	48.6, CH	4.51 (m)	2′,6′	129.6, CH	7.10–7.30 (m)
β	40.5, CH2	1.40 (m)	3′,5′	128.6, CH	7.10–7.30 (m)
		1.20 (m)	4′	126.6, CH	7.10–7.30 (m)
γ-CH	24.6, CH	1.62 (m)	Asp5
δ-CH3	23.8, CH3	0.91 (d, 7.0)	C=O	176.2, C
δ-CH3	21.3, CH3	0.93 (d, 7.0)	NH		7.95 (d, 8.4)
trans-Pro3			α	47.3, CH	5.23 (m)
C=O	171.7, C		β	36.5, CH2	3.22 (m)
α	61.0, CH	3.92 (t, 7.0)			2.05 (d, 3.5)
β	29.3, CH2	1.85 (m)	C=O	177.2, C
		1.67 (m)	Ile6
γ	25.4, CH2	2.01 (m)	C=O	167.0, C
		1.83 (m)	α	61.5, CH	4.19 (d, 11.9)
δ	47.5, CH2	3.68 (m)	β	30.4, CH	2.68 (m)
		3.42 (m)	γ-CH3	16.1, CH3	0.87 (t, 7.7)
			γ-CH2	24.3, CH2	1.38 (m)
					0.95 (m)
			δ-CH3	10.5, CH3	0.79 (d, 7.7)

). HSQC, HMBC, and COSY spectra demonstrated the presence of six amino acid residues including aspartic acid (D), phenylalanine (F), glycine (G), isoleucine (I), leucine (L), and proline (P). Moreover, HMBC, COSY, and ROESY spectra indicated that the sequence and connectivity of the hexapeptide was cyclo-G-L-P-F-D-I (Figure 2). The HMBC correlations between Hα (δ_H 4.19) of Ile⁶ and two carbonyls (δ_C 176.2 and 177.2) of Asp⁵ showed that dehydration of NH-Ile⁶ and COOH-Asp⁵ formed an additional five membered ring system (pyrrolidine-2,5-dione). The amino acid sequence in 2 was further supported by the fragmentation pattern of ESI–MS/MS data, in which the preferred ring-opening of 2 occurred at the amide bond between leucine and proline (Figure S19). The geometry of amide bond of Pro³ residue in 2 was assigned the trans configuration, on the basis of the difference of the ¹³C NMR chemical shift (Δδ_Cβ-Cγ = 3.9 ppm) [21,22,23] as well as the ROE correlation between the Hα (δ_H 4.51) of Leu² and the Hδ (δ_H 3.68 and 3.42) of Pro³ residue. In addition, the absolute configuration of six amino acid residues in 2 were all assigned as L, which was determined by HPLC analysis of the acid hydrolysate after Marfey’s derivatization (Figure S20). Therefore, dianthiamide B (2) was determined as cyclo-(Gly¹-l-Leu²-l-trans-Pro³-l-Phe⁴-l-Asp⁵-l-Ile⁶).

Dianthiamide C (3) was obtained as a yellow amorphous powder, showed a molecular formula of C₃₇H₅₇N₇O₈ as determined by its HRESI–TOF–MS data (m/z 750.4165 [M + Na]⁺; calcd 750.4160). The ¹³C and HSQC NMR data of 3 displayed the presence of 37 carbon signals including seven amide carbonyls, as well as seven α-amino acid carbons, suggesting a heptapeptide (

Table 3. ¹H and ¹³C NMR data for dianthiamide C (3) (DMSO-d₆, 700 MHz, d in ppm, J in Hz).

Position	δC	δH (J in Hz)	Position	δC	δH (J in Hz)
Gly1			Phe5
C=O	169.1, C		C=O	170.7, C
NH		8.91 (t, 4.9)	NH		7.43 (d, 9.8)
α	43.2, CH	4.00 (m)	α	54.6, CH	4.38 (m)
		3.33 (m)	β	38.4, CH2	3.21 (dd, 13.3, 3.5)
Leua2					2.62 (t, 13.3)
C=O	171.2, C		Phe-1′	138.4, C
NH		8.10 (d, 10.5)	2′,6′	129.4, CH	7.18–7.31 (m)
α	53.0, CH	4.52 (m)	3′,5′	128.6, CH	7.18–7.31 (m)
β	43.8, CH2	1.42 (m)	4′	126.8, CH	7.18–7.31 (m)
		1.34 (m)	Ile6
γ-CH	24.5, CH	1.55 (m)	C=O	171.6, C
δ-CH3	22.9, CH3	0.87 (d, 6.3)	NH		7.01 (d, 9.1)
δ-CH3	22.2, CH3	0.84 (d, 6.3)	α	56.4, CH	4.35 (m)
Ser3			β	37.9, CH	1.71 (m)
C=O	168.9, C		γ-CH3	15.1, CH3	0.82 (t, 7.7)
NH		8.73 (d, 7.0)	γ-CH2	24.7, CH2	1.39 (m)
α	54.2, CH	4.89 (br s)			1.00 (m)
β	61.9, CH2	4.24 (m)	δ-CH3	11.0, CH3	0.81 (d, 7.7)
		3.71 (d, 10.5)	Leub7
trans-Pro4			C=O	172.3, C
C=O	170.5, C		NH		8.71 (br s)
α	62.2, CH	3.98 (m)	α	54.2, CH	3.87 (m)
β	29.0, CH2	1.97 (m)	β	40.1, CH2	1.47 (m)
		1.24 (m)	γ-CH	24.4, CH	1.54 (m)
γ	25.8, CH2	1.79 (m)	δ-CH3	23.4, CH3	0.86 (m)
		1.68 (m)	δ-CH3	22.6, CH3	0.93 (m)
δ	47.8, CH2	3.91 (m)
		3.42 (m)

). Full assignments of ¹H and ¹³C NMR data for each amino acid residue were accomplished by combined analysis of COSY, HSQC, and HMBC spectra and suggested that 3 was composed of seven amino acid such as phenylalanine (F), glycine (G), isoleucine (I), two leucines (L_a and L_b), proline (P), and serine (S) residue (Table 3). The HMBC and ROESY spectra indicated that the amino acid sequence was cyclo-G-L_a-S-P-F-I-L_b (Figure 2), which was further confirmed by ESI–MS/MS fragmentation analysis (Figure S29). The observed Δδ_Cβ-Cγ value (3.2 ppm) of the Pro⁴ and the ROE correlation from the Hα (δ_H 4.89) of Ser³ to the Hδ (δ_H 3.91 and 3.42) of Pro⁴ revealed that the geometry of Pro⁴ of 3 was a trans configuration [21,22,23]. The absolute configuration of 3 was determined by Marfey’s method [24,25,26,27], which indicated that all the amino acids were L configuration (Figure S30). Therefore, dianthiamide C (3) was confirmed as cyclo-(Gly¹-l-Leu²-l-Ser³-l-trans-Pro⁴-l-Phe⁵-l-Ile⁶-l-Leu⁷).

Dianthiamide D (4), a yellow amorphous powder, gave the molecular formula C₃₆H₆₀N₈O₈, based on the HRESI–TOF–MS data (m/z 755.4448 [M + Na]⁺; calcd 755.4426). Detailed analyses of the 1D and 2D (COSY, HSQC, and HMBC) NMR data revealed that 4 was a octapeptide containing alanine (A), glycine (G), isoleucine (I), two prolines (P_a and P_b) and three valines (V_a, V_b, and V_c) residues (

Table 4. ¹H and ¹³C NMR data for dianthiamide D (4) (DMSO-d₆, 700 MHz, d in ppm, J in Hz).

Position	δC	δH (J in Hz)	Position	δC	δH (J in Hz)
Gly1			trans-Pro5
C=O	168.4, C		C=O	170.7, C
NH		8.97 (t, 4.9)	α	62.0, CH	4.29 (m)
α	43.2, CH	3.84 (dd, 14.7, 4.9)	β	27.6, CH2	2.11 (m)
		3.33 (dd, 14.7, 4.2)			1.91 (m)
Ala2			γ	25.0, CH2	1.89 (m)
C=O	173.1, C				1.79 (m)
NH		7.30 (d, 7.0)	δ	48.2, CH2	3.79 (m)
α	47.2, CH	4.62 (t, 7.0)			3.62 (m)
β	19.6, CH3	1.42 (d, 7.0)	Val6
Val3			C=O	171.6, C
C=O	171.4, C		NH		8.24 (d, 7.0)
NH		7.99 (d, 4.9)	α	60.0, CH	3.89 (dd, 7.0, 4.2)
α	60.8, CH	3.71 (t, 4.9)	β	29.3, CH	2.23 (m)
β	28.7, CH	2.14 (m)	γ-CH3	20.1, CH3	0.90 (m)
γ-CH3	19.9, CH3	0.92 (d, 6.3)	γ-CH3	18.4, CH3	0.85 (d, 5.6)
γ-CH3	19.0, CH3	0.93 (d, 6.3)	Val7
Ile4			C=O	169.8, C
C=O	173.0, C		NH		7.16 (d, 7.0)
NH		6.70 (d, 7.7)	α	55.6, CH	4.43 (t, 7.0)
α	54.7, CH	4.48 (d, 7.7)	β	31.1, CH	2.02 (m)
β	35.9, CH	1.81 (m)	γ-CH3	19.9, CH3	0.86 (m)
γ-CH3	15.6, CH3	0.88 (t, 5.6)	γ-CH3	18.4, CH3	0.75 (d, 5.6)
γ-CH2	24.3, CH2	1.49 (m)	trans-Pro8
		1.08 (m)	C=O	173.0, C
δ-CH3	11.0, CH3	0.83 (d, 7.0)	α	60.8, CH	4.18 (m)
			β	29.4, CH2	2.08 (m)
					1.74 (m)
			γ	25.4, CH2	1.99 (m)
					1.84 (m)
			δ	47.9, CH2	3.76 (m)
					3.53 (m)

). The amino acid sequence of 4 was established as cyclo-G-A-V_a-I-P_a-V_b-V_c-P_b by analysis of HMBC and ROESY data (Figure 2). This conclusion was also supported by the ESI–MS/MS sequence analysis (Figure S39). The observed Δδ_Cβ-Cγ values of the Pro_a⁵ (2.6 ppm) and Pro_b⁸ (4.0 ppm) and the ROE correlations from the Hα of Ile⁴ (δ_H 4.48) to the Hδ of Pro_a⁵ (δ_H 3.79 and 3.62), and from the Hα of Val_c⁷ (δ_H 4.43) to the Hδ of Pro_b⁸ (δ_H 3.76 and 3.53) indicated that the geometry of both Pro_a⁵ and Pro_b⁸ of 4 were trans configuration [21,22,23]. Moreover, the absolute configuration of 4 was assigned by Marfey’s method [24,25,26,27], which indicated that all the amino acids had L configuration (Figure S40). Therefore, dianthiamide D (4) was established as cyclo-(Gly¹-l-Ala²-l-Val_a³-l-Ile⁴-l-trans-Pro_a⁵-l-Val_b⁶-l-Val_c⁷-l-trans-Pro_b⁸).

Dianthiamide E (5), a yellow amorphous powder. Its molecular formula of C₃₆H₅₄N₈O₉ was determined from the HRESI–TOF–MS data (m/z 743.4097 [M + H]⁺; calcd for 743.4086). Analysis of 1D and 2D (COSY, HSQC, and HMBC) NMR data (

Table 5. ¹H and ¹³C NMR data for dianthiamide E (5) (DMSO-d₆, 700 MHz, d in ppm, J in Hz).

Position	δC	δH (J in Hz)	Position	δC	δH (J in Hz)
Gly1			Thr5
C=O	169.2, C		C=O	170.7, C
NH		8.01 (m)	NH		7.57 (d, 9.1)
α	43.6, CH2	4.02 (m)	α	57.0, CH	4.67 (m)
		3.31 (m)	β	68.7, CH	4.25 (m)
Asn2			γ-CH3	19.4, CH3	1.03 (d, 6.3)
C=O	171.0, C		OH		5.26 (d, 6.3)
NH		7.15	Ile6
α	48.8, CH	4.79 (dd, 14.0, 7.0)	C=O	171.1, C
β	37.2, CH2	2.75 (m)	NH		8.00 (m)
		2.57 (m)	α	59.2, CH	3.95 (t, 5.6)
C=O	172.2, C		β	35.7, CH	1.88 (m)
NH2		7.75 (br s)	γ-CH3	15.9, CH3	0.70 (d, 7.0)
		7.20 (m)	γ-CH2	23.9, CH2	0.97 (m)
trans-Pro3			δ-CH3	11.8, CH3	0.68 (d, 7.0)
C=O	171.5, C		Phe7
α	62.1, CH	4.11 (t, 7.7)	C=O	171.6, C
β	29.7, CH2	2.20 (m)	NH		7.78 (d, 2.1)
		1.71 (m)	α	54.9, CH	4.41 (m)
γ	25.3, CH2	1.92 (m)	β	37.3, CH2	3.13 (dd, 14.0, 5.6)
δ	47.5, CH2	3.73 (m)			2.84 (dd, 14.0, 9.1)
		3.56 (m)	Phe-1′	138.0, C
Leu4			2′,6′	129.4, CH	7.17–7.29 (m)
C=O	172.2, C		3′,5′	128.7, CH	7.17–7.29 (m)
NH		8.39 (br s)	4′	126.8, CH	7.17–7.29 (m)
α	52.4, CH	4.00 (m)
β	39.2, CH2	1.79 (m)
		1.63 (m)
γ	25.1, CH	1.54 (m)
δ-CH3	23.7, CH3	0.88 (d, 7.0)
δ-CH3	21.3, CH3	0.82 (d, 7.0)

and Figure 2) as well as ESI–MS/MS sequence data (Figure S49) demonstrated that the seven amino acid residues were phenylalanine (F), glycine (G), isoleucine (I), asparagine (N), leucine (L), proline (P), and threonine (T). The sequence of these amino acid residues was assigned as cyclo-G-N-P-L-T-I-F by the observed HMBC and ROESY data (Figure 2).

The small difference of the Δδ_Cβ-Cγ values of the Pro³ (4.4 ppm) and the ROE correlations between the Hα of Asn² (δ_H 4.79) and the Hδ of Pro³ (δ_H 3.73 and 3.56) revealed that the geometry of Pro³ of 5 was a trans [21,22,23]. Furthermore, Marfey’s analysis assigned L configurations to all the amino acid residues in 5 (Figure S50) [24,25,26,27]. Therefore, dianthiamide E (5) was established as cyclo-(Gly¹-l-Asn²-l-trans-Pro³-l-Leu⁴-l-Thr⁵-l-Ile⁶-l-Phe⁷).

Recently, it has been reported that cyclic peptides isolated from the genus Dianthus exhibited cytotoxic activity against several cancer cell lines [5,6,18]. Therefore, all isolates were tested for their cytotoxic activity against human non-small cell lung cancer A549 and human stomach adenocarcinoma MKN-28 cells, with docetaxel as a positive control. However, dianthiamide A (1) only showed weak activity against A549 cell line with IC₅₀ value of 47.9 μM, and docetaxel was used as a positive control (IC₅₀: 0.08 μM). The other compounds 2–5 were inactive against A549 and MKN-28 cells (IC₅₀: >200 μM).

3. Materials and Methods

3.1. Chemicals

HPLC grade acetonitrile was purchased from m Fisher Chemical (Loughborough, UK) and all other chromatographic solvents were purchased from Duksan Pure Chemicals Co., Cheongju, Korea). Paclitaxel as a positive control was obtained from LC Laboratories (Woburn, MA, USA). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA).

3.2. General Experimental Procedures

Optical rotations were measured on a JASCO DIP-1000 polarimeter. UV spectra were recorded on a JASCO UV-550 spectrophotometer. ECD spectra were obtained on a JASCO J-715 spectrometer, and IR spectra were measured on a JASCO FT-IR 4100 spectrometer (JASCO, Tokyo, Japan). NMR spectra were recorded on a Bruker AVANCE 700 MHz spectrometer (Bruker, MA, USA) using DMSO-d₆ as solvent. ESI–MS and HRESI–TOF–MS were obtained with LCQ Fleet (Thermo Fisher Scientific, San Jose, CA, USA) and maXis 4G mass spectrometers (Bruker, Bremen, Germany), respectively. Column chromatography was performed on silica gel (70–230 mesh, Merck, Darmstadt, Germany) and Lichroprep RP-18 (40–63 μm, Merck, Darmstadt, Germany). MPLC was performed on a Biotage Isolera Prime chromatography system (Biotage, Uppsala, Sweden). Preparative HPLC was performed using Waters HPLC system equipped with two Waters 515 pumps with a 2996 photodiode-array detector (Waters Corporation, Milford, MA, USA) using an YMC J’sphere ODS-H80 column (4 μm, 150 × 20 mm, i.d., Kyoto, Japan, flow rate 6 mL/min). TLC was performed using precoated silica gel 60 F₂₅₄ (0.25 mm, Merck, Darmstadt, Germany) plates, and spots were detected by a 10% vanillin-H₂SO₄ in water spray reagent.

3.3. Plant Material

The dried whole plants of Dianthus chinensis L. (Caryophyllaceae) were purchased from Kyungdong herbal market in Seoul, Korea, in June 2014. A voucher specimen (CBNU-2014-06-DC) was authenticated by B.Y.H. and deposited at the Herbarium of the College of Pharmacy, Chungbuk National University, Korea.

3.4. Isolation and Purification of Compounds 1–5

The dried and powdered whole plants of D. chinensis (3.0 kg) were extracted with MeOH (3 × 16 L) at room temperature. The extract was evaporated under reduced pressure, and the residue (470 g) was suspended in water and partitioned successively with n-hexane (2 × 1.5 L), CH₂Cl₂ (2 × 1.5 L), and EtOAc (2 × 1.5 L). The CH₂Cl₂-soluble fraction (13 g) was separated by MPLC with Lichroprep RP-18 column and eluted with MeOH-H₂O gradient system (10:90 to 100:0) to give eleven fractions (DCC1-DCC11). DCC1 (1.2 g) was separated on a silica gel column and eluted with CH₂Cl₂-MeOH gradient (from 100:0 to 0:100, 400 mL for each step) to obtain seven fractions (DCC1-1-DCC1-7) by MPLC. DCC1-4 (90 mg) was further purified by preparative HPLC (Waters system, YMC J’sphere ODS-H80, 150 × 20 mm i.d., MeCN-H₂O, 30:70 to 60:40, flow rate 6 mL/min) to yield compound 1 (t_R = 20.1 min, 15 mg). DCC7 (1.0 g) was subjected to silica gel column chromatography and eluted with CH₂Cl₂-MeOH (from 100:0 to 0:100, 400 mL for each step) to give seven fractions (DCC7-1-DCC7-7) by MPLC. DCC7-4 (90 mg) was further purified by preparative HPLC (MeCN-H₂O, 35:65 to 65:35) to yield compound 2 (t_R = 21.4 min, 17 mg). DCC9 (2.2 g) was separated on a silica gel column and eluted with CH₂Cl₂-MeOH gradient (from 100:0 to 0:100, 400 mL for each step) to obtain nine fractions (DCC9-1-DCC9-9) by MPLC. DCC9-7 (130 mg) was further purified by preparative HPLC (MeCN-H₂O, 30:70 to 60:40) to afford compounds 3 (t_R = 18.9 min, 6 mg) and 5 (t_R = 23.1 min, 4 mg). DCC9-9 (90 mg) was further purified by preparative HPLC (MeCN-H₂O, 30:70 to 60:40) to afford compound 4 (t_R = 21.9 min, 5 mg).

3.5. Characterization of Compounds 1–5

Dianthiamide A (1, cyclo-(Gly¹-l-Phe²-l-Leu³-l-trans-Pro_a⁴-l-cis-Pro_b⁵-l-Ile⁶-l-Asn⁷)), Yellow amorphous powder; [α]²⁵_D -41.2 (c 0.05, MeOH); UV (MeOH) λ_max (log ε) 203 (3.65) nm; ECD (MeOH) λ_max (Δε) 201 (−10.9), 210 (−6.5), 219 (−7.8) nm; IR υ_max (film) 3330, 2944, 1657, 1530, 1454 cm⁻¹; ¹H NMR (700 MHz, DMSO-d₆) and ¹³C NMR (175 MHz, DMSO-d₆), see Table 1; ESI–MS m/z 739 [M + H]⁺; HRESI–TOF–MS m/z 739.4144 [M + H]⁺ (calcd for C₃₇H₅₅N₈O₈, 739.4137).

Dianthiamide B (2, cyclo-(Gly¹-l-Leu²-l-trans-Pro³-l-Phe⁴-l-Asp⁵-l-Ile⁶)), Yellow amorphous powder; [α]²⁵_D -20.0 (c 0.05, MeOH); UV (MeOH) λ_max (log ε) 202 (3.70) nm; ECD (MeOH) λ_max (Δε) 201 (−3.2), 205(−1.5), 217 (−4.2) nm; IR υ_max (film) 3312, 2972, 1644, 1530, 1448 cm⁻¹; ¹H NMR (700 MHz, DMSO-d₆) and ¹³C NMR (175 MHz, DMSO-d₆), see Table 2; ESI–MS m/z 647 [M + Na]⁺; HRESI–TOF–MS m/z 647.3161 [M + Na]⁺ (calcd for C₃₂H₄₄N₆NaO₇, 647.3163).

Dianthiamide C (3, cyclo-(Gly¹-l-Leu²-l-Ser³-l-trans-Pro⁴-l-Phe⁵-l-Ile⁶-l-Leu⁷)), Yellow amorphous powder; [α]²⁵_D -45.2 (c 0.05, MeOH); UV (MeOH) λ_max (log ε) 204 (3.24) nm; ECD (MeOH) λ_max (Δε) 207 (+1.4), 226 (−8.6) nm; IR υ_max (film) 3311, 2944, 1644, 1530, 1462 cm⁻¹; ¹H NMR (700 MHz, DMSO-d₆) and ¹³C NMR (175 MHz, DMSO-d₆), see Table 3; ESI–MS m/z 750 [M + Na]⁺; HRESI–TOF–MS m/z 750.4165 [M + Na]⁺ (calcd for C₃₇H₅₇N₇NaO₈, 750.4160).

Dianthiamide D (4, cyclo-(Gly¹-l-Ala²-l-Val_a³-l-Ile⁴-l-trans-Pro_a⁵-l-Val_b⁶-l-Val_c⁷-l-trans-Pro_b⁸)), Yellow amorphous powder; [α]²⁵_D -37.8 (c 0.05, MeOH); UV (MeOH) λ_max (log ε) 210 (3.44) nm; ECD (MeOH) λ_max (Δε) 201 (+9.1), 219 (−7.1) nm; IR υ_max (film) 3312, 2943, 1644, 1548, 1454 cm⁻¹; ¹H NMR (700 MHz, DMSO-d₆) and ¹³C NMR (175 MHz, DMSO-d₆), see Table 4; ESI–MS m/z 755 [M + Na]⁺; HRESI–TOF–MS m/z 755.4448 [M + Na]⁺ (calcd for C₃₆H₆₀N₈NaO₈, 755.4426).

Dianthiamide E (5, cyclo-(Gly¹-l-Asn²-l-trans-Pro³-l-Leu⁴-l-Thr⁵-l-Ile⁶-l-Phe⁷)), Yellow amorphous powder; [α]²⁵_D -41.2 (c 0.05, MeOH); UV (MeOH) λ_max (log ε) 203 (3.47) nm; ECD (MeOH) λ_max (Δε) 201 (−5.8) nm; IR υ_max (film) 3309, 2942, 1741, 1644, 1548 cm⁻¹; ¹H NMR (700 MHz, DMSO-d₆) and ¹³C NMR (175 MHz, DMSO-d₆), see Table 5; ESI–MS m/z 743 [M + H]⁺; HRESI–TOF–MS m/z 743.4097 [M + H]⁺ (calcd for C₃₆H₅₅N₈O₉, 743.4086).

3.6. Absolute Configuration of Amino Acids in 1–5 Using Marfey’s Method

Compounds 1–5 (0.5 mg) were hydrolyzed in 1 mL of 6 N HCl at 105 °C for 12 h. After cooling to room temperature, the hydrolysate was evaporated to dryness and redissolved in 200 μL of water and 1 M NaHCO₃ (20 μL). A solution of N-α-(2,4-dinitro-5-fluorophenyl)-l-alaninamide (l-FDAA, Marfey’s reagent, Sigma, 100 μL, 1%) in acetone was added to each reaction vial. The reaction mixture was heated at 37 °C for 1 h, quenched by adding 1 N HCl (20 μL), and then dissolved in CH₃CN (800 μL). A volume of 5 μL of the FDAA derivatives were analyzed by LC/MS (YMC UltraHT Pro C₁₈, S-2 μm, 12 nm, 50 × 2.0 mm, flow rate: 0.2 mL/min) at RT, and monitored by UV absorption at 340 nm. Aqueous CH₃CN containing 0.1% TFA was used as the mobile phase in a gradient mode (10–50% CH₃CN for 0–40 min). From each standard, 50 mM aqueous solution of d- or l-amino acid (Ala, Asp, Phe, Ile, allo-Ile, Leu, Asn, Pro, Ser, Thr, allo-Thr, and Val) were taken, and 1 M NaHCO₃ (20 μL) and a solution of N-α-(2,4-dinitro-5-fluorophenyl)-l-alaninamide (l-FDAA, Marfey’s reagent, Sigma, 100 μL, 1%) in acetone was added. The reaction mixture was heated at 37 ^oC for 1 h, quenched by adding 1 N HCl (20 μL), and then dissolved in CH₃CN (800 μL). A volume of 5 μL of the FDAA derivatives were analyzed by LC/MS (YMC UltraHT Pro C₁₈, S-2 μm, 12 nm, 50 × 2.0 mm, flow rate: 0.2 mL/min) at RT and monitored by UV absorption at 340 nm. Aqueous CH₃CN containing 0.1% TFA was used as the mobile phase in a gradient mode (10–50% CH₃CN for 0–40 min). The following retention times (min) were observed for the l-FDAA derivatives of the standards, respectively: 19.5 (l-Ala) and 22.7 (d-Ala), 15.0 (l-Asp) and 18.1 (d-Asp), 29.7 (l-Phe) and 33.4 (d-Phe), 29.5 (l-Leu) and 34.4 (d-Leu), 30.3 (l-Ile), 31.0(l-allo-Ile) and 35.2 (d-Ile), 20.8 (l-Pro) and 22.1 (d-Pro), 13.6 (l-Ser) and 14.5 (d-Ser), 14.7 (l-Thr), 15.2 (l-allo-Thr) and 18.2 (d-Thr), and 25.4 (l-Val) and 29.6 (d-Val).

3.7. Cytotoxicity Assay

Human non-small cell lung cancer A549 and human stomach adenocarcinoma MKN-28 cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). A549 and MKN-28 cells were cultured as monolayers in RPMI1640 medium containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin at 37 °C in a humidified atmosphere of 5% CO₂. Growth-inhibitory effect of the isolated compounds on A549 cells and MKN-28 cells was evaluated using MTT assay [28]. Briefly, 5 × 10³ cells of A549 cells or MKN-28 cells were seeded in each well of a 96-well plate, respectively, and incubated for 24 h. A549 cells or MKN-28 cells were then treated with various concentrations of compounds 1–5. The concentration range of the compound tested for the evaluation of the cytotoxic activity was 5–200 µM. After incubation of 48 h, MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution was added to each well and the plate was incubated for 4 h. The medium in each well was replaced with dimethyl sulfoxide (DMSO) to dissolve blue formazan crystals. The absorbance at 540 nm was measured using microplate reader (Molecular devices; SpectraMax, CA, USA). All data processing and IC₅₀ values were analyzed using GraphPad Prism v.5 (GraphPad Software, La Jolla, CA, USA). Docetaxel was used as a positive control with an IC₅₀ value of 0.08 μM on A549 cells [29].

4. Conclusions

We report the isolation and structure determination of five undescribed orbitides, dianthiamides A–E, from the whole plants of D. chinensis. The previously reported orbitides isolated from the genus Dianthus tend to have five to six amino acid residues [4,5,6,7,8,9]. All orbitides in this study, however, are characteristic of the presence of six to eight amino acids, while they are featured by containing at least one proline residue. All isolates were tested for their cytotoxic activity, and dianthiamide A (1) exhibited weak activity against the A549 cell line. Furthermore, from a chemotaxonomical point of view, it is noteworthy that this finding expands the orbitides diversity in the genus Dianthus.