Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves
Abstract
:1. Introduction
2. Results and Analysis
2.1. Optimization of Extraction Process of Flavonoids from Perilla leaves
2.1.1. Single Factor Test Results
Effect of Liquid–Solid Ratio on Extraction Rate of Flavonoids from Perilla leaves
Effect of Ethanol Volume Fraction on Extraction Yield of Total Flavonoids from Perilla leaves
Effect of Ultrasonic Temperature on Extraction of Flavonoids from Perilla leaves
Effect of Ultrasonic Time on Extraction of Flavonoids from Perilla leaves
Effect of Flash Time on Extraction of Flavonoids from Perilla leaves
2.1.2. Box-Behnken Test Results
2.2. Separation and Purification of Flavonoids from Perilla leaves
2.2.1. Static Adsorption and Resolution of Resin
2.2.2. Separation and Purification of Flavonoids from Perilla leaves
2.2.3. Optimization of the Enrichment Process of HPD450 Macroporous Resin
The Influence of Sample Amount on the Resolution Rate of HPD450 Macroporous Resin
Influence of Eluent Concentration on Resolution Rate of HPD450 Macroporous Resin
Effect of Eluate Volume on the Concentration of Flavonoids in Perilla leaves
2.3. Identification of Flavonoid Compounds in Perilla leaves
2.4. Study on Antioxidant Activity of Flavonoids from Perilla leaves
2.4.1. DPPH Free Radical Scavenging Activity of Perilla leaf Flavonoids
2.4.2. Hydroxyl Radical Scavenging Ability of Perilla leaf Flavonoids
2.4.3. ABTS Free Radical Scavenging Ability of Perilla leaf Flavonoids
2.4.4. Effect of Perilla leaf Flavonoids on Fe3+ Reducing Power
3. Materials and Methods
3.1. Materials and Instruments
3.2. Experimental Methods
3.2.1. Extraction Technology of Flavonoids from Perilla leaves
3.2.2. Optimization of Extraction Process of Flavonoids from Perilla leaves
3.2.3. Isolation and Purification of Flavonoids from Perilla leaves
Pretreatment of Resin
Resin Screening
Dynamic Curve of HPD450 Resin
Optimization of Enrichment Process for HPD450 Resin
3.2.4. Identification of Flavonoids in Perilla leaves
3.2.5. Study on Antioxidant Activity
DPPH Free Radical Scavenging Experiment
Determination of Hydroxyl Radical Scavenging Ability
ABTS+• Scavenging Effect of Free Radicals
Determination of Reducing Power
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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The No. | Liquid/Material Ratio (mL/g) | Ethanol Volume Fraction (%) | Ultrasonic Temperature (°C) | Flavonoids Extraction Yield (mg/g) |
---|---|---|---|---|
1 | 20 | 50 | 70 | 9.04 |
2 | 15 | 60 | 70 | 6.77 |
3 | 20 | 60 | 60 | 9.82 |
4 | 15 | 70 | 60 | 6.57 |
5 | 20 | 70 | 50 | 8.99 |
6 | 25 | 60 | 70 | 9.35 |
7 | 20 | 60 | 60 | 9.76 |
8 | 20 | 60 | 60 | 9.78 |
9 | 20 | 70 | 70 | 9.19 |
10 | 15 | 50 | 60 | 6.75 |
11 | 25 | 50 | 60 | 9.03 |
12 | 25 | 60 | 50 | 9.08 |
13 | 20 | 60 | 60 | 9.81 |
14 | 25 | 70 | 60 | 9.43 |
15 | 20 | 60 | 60 | 9.79 |
16 | 15 | 60 | 50 | 6.66 |
17 | 20 | 50 | 50 | 9.06 |
Sources of Variance | Sum of Squares | Degrees of Freedom | The Mean Square | The F Value | p Values | Significant |
---|---|---|---|---|---|---|
model | 23.90 | 9 | 2.66 | 1526.18 | <0.0001 | ** |
A | 12.85 | 1 | 12.85 | 7386.47 | <0.0001 | |
B | 0.011 | 1 | 0.011 | 6.47 | 0.0385 | |
C | 0.039 | 1 | 0.039 | 22.53 | 0.0021 | |
AB | 0.084 | 1 | 0.084 | 48.33 | 0.0002 | |
AC | 6.4 × 10−3 | 1 | 6.4 × 10−3 | 3.68 | 0.0966 | |
BC | 0.012 | 1 | 0.012 | 6.95 | 0.0336 | |
A2 | 9.17 | 1 | 9.17 | 5271.81 | <0.0001 | |
B2 | 0.58 | 1 | 0.58 | 333.07 | <0.0001 | |
C2 | 0.52 | 1 | 0.52 | 298.13 | <0.0001 | |
residual | 0.012 | 7 | 1.74 × 10−3 | |||
Loss of quasi | 9.9 × 10−3 | 3 | 3.3 × 10−3 | 5.79 | 0.0614 | |
Pure error | 2.28 × 10−3 | 4 | 5.7 × 10−4 | |||
The sum of the | 23.91 | 16 |
Factors | The Level of Factors | ||
---|---|---|---|
−1 | 0 | 1 | |
Liquid/material ratio (mL/g) | 15:1 | Those days | 25:1 |
Ethanol volume fraction (%) | 50 | 60 | 70 |
Ultrasonic temperature (°C) | 50 | 60 | 70 |
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Li, H.; Lin, J.; Bai, B.; Bo, T.; He, Y.; Fan, S.; Zhang, J. Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves. Molecules 2023, 28, 7273. https://doi.org/10.3390/molecules28217273
Li H, Lin J, Bai B, Bo T, He Y, Fan S, Zhang J. Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves. Molecules. 2023; 28(21):7273. https://doi.org/10.3390/molecules28217273
Chicago/Turabian StyleLi, Hui, Jiayu Lin, Baoqing Bai, Tao Bo, Yufei He, Shanhong Fan, and Jinhua Zhang. 2023. "Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves" Molecules 28, no. 21: 7273. https://doi.org/10.3390/molecules28217273
APA StyleLi, H., Lin, J., Bai, B., Bo, T., He, Y., Fan, S., & Zhang, J. (2023). Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves. Molecules, 28(21), 7273. https://doi.org/10.3390/molecules28217273