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Article

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

by
Mousa Alreshidi
1,
Mohammad A. Abdulhakeem
1,
Riadh Badraoui
1,2,
Giuseppe Amato
3,
Lucia Caputo
3,*,
Laura De Martino
3,
Filomena Nazzaro
4,
Florinda Fratianni
4,
Carmen Formisano
5,
Vincenzo De Feo
3,* and
Mejdi Snoussi
1,6
1
Department of Biology, College of Science, University of Ha’il, Hail 2440, Saudi Arabia
2
Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia
3
Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy
4
Institute of Food Sciences, CNR, 83100 Avellino, Italy
5
Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, 80131 Napoli, Italy
6
Laboratory of Genetics, Biodiversity and Valorization of Bio-Resources (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Haddad, BP74, Monastir 5000, Tunisia
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(21), 7439; https://doi.org/10.3390/molecules28217439
Submission received: 12 October 2023 / Revised: 31 October 2023 / Accepted: 1 November 2023 / Published: 5 November 2023
(This article belongs to the Special Issue Natural Products: Isolation, Analysis and Biological Activity)
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Abstract

:
Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of Pulicaria incisa (Lam.) DC. We also reported the molecular interaction between identified molecules and several receptors associated with antimicrobial and antibiofilm activities. A total of seventeen and thirteen compounds were identified in aqueous and methanolic extracts of P. incisa, respectively. The methanolic extract yielded a higher total content of polyphenols and flavonoids of about 84.80 ± 2.8 mg GAE/g and 28.30 ± 1.2 mg QE/g, respectively. Significant antibacterial activity was recorded for both extracts, with minimum inhibitory concentration (MIC) values ranging from 30 to 36 µg/mL, and the result was comparable to the reference antibiotic control. Antibiofilm assays revealed that both extracts were able to reduce the attachment of bacterial cells to 96-well plates, but the highest antibiofilm activity was recorded against Staphylococcus aureus. The methanolic extract also showed anti-enzymatic potency and high antioxidant activity, as demonstrated by all assays used, including DPPH, FRAP, and ABTS. These results were further validated by in silico approaches, particularly the molecular interaction of the identified compounds with the targeted receptors. These findings present P. incisa as a significant source of antibacterial, antibiofilm, antioxidant, and anti-enzymatic molecules.

1. Introduction

Pulicaria species (Asteraceae) are widely distributed in the Arabian region, including Saudi Arabia, as the plant survives in saline and arid environments [1,2]. The species have acclimatized to grow in these environments by inducing various morphological and biochemical alterations to combat undesirable conditions [3,4]. Some Pulicaria species are sometimes added to hot beverages, particularly tea, in some regions in the north of Saudi Arabia and have been comprehensively used in traditional medicine as an insect repellent and epilepsy remedy and for other economic purposes. They are habitually used as a medicine for influenza, gastrointestinal diseases, and joint inflammation [5]. The essential oil from Pulicaria species, particularly P. undulata, has been shown to have anticancer activities and substantial antioxidant properties [6].
Pulicaria incisa (Lam.) DC. has been characterized as a potential agent for treating heart diseases because it has a high quantity of unsaturated fatty acids able to reduce total triglyceride and cholesterol levels [7]. This species is characterized by its aromatic odor and essential oil content [6]. Indeed, P. incisa has been used as an ingredient of perfume, a tonic, a hypoglycemic, and an antispasmodic. It contains various biological molecules that may contribute to its ability to grow in arid environments; thus, it could be highly useful for antimicrobial activities due to the synthesis of the chemical constituents of arid plants. P. incisa was recently demonstrated to have efficient antioxidant activity, and based on this evidence, further evaluation of its potential uses as a drug agent for neurodegenerative and brain illnesses characterized by oxidative stress has been suggested [7,8]. The plant has yet to be comprehensively studied, but a few studies have reported its anti-inflammatory, antioxidant, and cytotoxic activities [9,10].
The exploration of bioactive molecules of plants with potential uses for treating human diseases, including diabetes, brain diseases, and microbial diseases, has been one of the most rigorous fields of scientific research. Therefore, natural products are increasingly used in the pharmaceutical industry as a foundation for bioactive molecules for the treatment of various human diseases. Furthermore, the investigation of herbs and plant-based products, as well as major pharmacological alternatives, remains essential. The biological activities, molecular docking, and dynamics of P. incisa have not yet been investigated, despite its assessed medicinal, biological, and environmental importance.
Therefore, this study aimed to characterize the chemical composition of P. incisa and subsequently identify potential bioactive molecules. This study also explored the antimicrobial, antibiofilm, antioxidants, and antidiabetic activities of aqueous and methanolic extracts of P. incisa using both in vitro and in silico approaches. The pharmacokinetic properties of the identified molecules in both the aqueous and methanolic extracts are described.

2. Results and Discussion

2.1. Chemical Analysis

LC-HRESIMS/MS analyses of the P. incisa extracts resulted in the separation and annotation of 17 components belonging to several classes of molecules (Table 1). Figure 1 reports a chromatogram of the two extracts. Compound 1 gave an [M + H]+ ion at m/z 294.1545, attributed to N-fructosyl (iso)leucine (C12H23O7N). The compound also yielded a major fragment at m/z 248.1492 (C11H22NO5), related to a loss of a carboxyl group, as reported by Pecio et al. [11], where the molecule was found in Iphiona mucronata (Forssk.) Asch. and Schweinf, a species belonging to the tribe Inuleae and chemotaxonomically close to Inula [11]. Another amino acid derivative was found in both the aqueous and methanolic extracts and identified as L-phenylalanine, a compound that yielded an [M + H]+ ion at m/z 166.0862 and has been frequently reported in the amino acid composition of different species of the Pulicaria genus [12,13]. Also in this case, the MS2 fragment at m/z 120.0806 was related to a loss of carboxyl group. Compound 3 gave an [M + H]+ ion at m/z 355.1019, which was attributed to an isomeric chlorogenic acid [9]. The analysis, based on the MS2 data, revealed a fragment at m/z 163.0391, related to the loss of quinic acid and also recently reported in the literature [14,15]. The compound was also found in other Pulicaria species [16].
The base peak at m/z 199.1328, observed in both chromatograms (Rt 7.52 mn), was attributed to the presence of (−)-hydroxy-dihydrobovolide. The compound showed an MS2 fragment at m/z 181.1222, corresponding to the loss of an OH group. The compound was previously found in the Asteraceae family [17]. Compounds 5 and 6 were two phenolic acids that gave base peaks at 339.0926 [M + H]+ and 369.1042 [M + H]+, respectively, corresponding to two protonated molecular forms of O-coumaroylquinic acid and feruloylquinic acid. The first acid gave an MS2 fragment with m/z 147.0438, related to a loss of quinic acid [18] (Yang et al., 2023); also, the second phenolic acid gave an MS2 fragment with m/z 177.0545, related to a loss of quinic acid. Both substances were previously identified in the same genus [18,19]. In addition, compound 7, eluting at 8.12 mn in both extracts, showed a base peak at 169.1222 [M + H]+ and was identified as 8-hydroxycarvotanacetone, previously reported in a dichloromethane extract from P. jaubertii [20]. An MS/MS spectrum showed a fragmented ion peak at m/z 151.1108 which corresponded to the loss of a water molecule and at m/z 109.0658, produced by the loss of a C3H6 group.
The first flavonoid, compound 8, eluted at 8.30 mn, gave a protonated molecular ion at m/z 465.1020, for which the molecular formula C21H20O12 was generated. The fragmentation of this compound, present only in the methanolic extract of P. incisa, was in agreement with isoquercetin, which gave an MS2 fragment at m/z 303.0496, corresponding to the protonated molecular ion of quercetin. Recently, a study reported the presence of this flavonoid in P. incisa [9].
The base peak at m/z 517.1329 [M + H]+, observed only in the methanolic extract (Rt 8.80), was attributed to the presence of an organic acid corresponding to (−)-3,5-dicaffeoylquinic acid. The fragmentation pathway revealed the presence of a peak at m/z 499.1226, corresponding to a loss of H2O, and a peak at m/z of 355.1019, corresponding to a protonated molecular ion of caffeoylquinic acid. Bakr and coworkers (2021) [9] reported the presence of this compound in the same species. Compound 10 gave an [M + H]+ ion at m/z 203.101792, attributed to calamenene, a sesquiterpene hydrocarbon usually found in plant essential oils. Moreover, the MS2 data analysis revealed a fragment at m/z 147.1165, corresponding to the losses of isopropyl and methyl groups. Recently, Askari et al. [21] reported the presence of the compound in the methanolic extract of P. undulata. The base peak at m/z 317.0652 [M + H]+, observed in both extracts (Rt 11.12), was attributed to the presence of rhamnetin. The fragment at m/z 299.0550 corresponded to the loss of H2O. The compound was previously found in the same species [19] with a similar fragmentation pattern. Compound 12 gave an [M + H]+ ion at m/z 331.0806, attributed to quercetin dimethyl ether. The fragment peaks observed in the MS spectra of the extracts showed the loss of a methyl group (15 amu), resulting in the fragmented ion at m/z 316.0573. Both quercetin 3,3′-dimethyl ether and quercetin 3,7-dimethyl ether were previously reported in P. incisa [22,23]. The base peak at m/z 287.0912 [M + H]+, observed only in the methanolic extract (Rt 11.77), was attributed to the presence of sakuranetin. The compound, previously reported in Inula viscosa L. [24], gave an MS2 fragment with m/z 167.0339, also reported in the literature [25], and the fragment corresponded to a loss of C8H6O [26].
Compound 14 gave an [M + H]+ ion at m/z 301.0705, corresponding to a protonated molecular form of chrysioerol, recently found in P. incisa by El-Sabagh and coworkers [19]. The compound produced a fragment with m/z 286.0784, related to the loss of a methyl group [27].
Compound 15 gave an [M + H]+ ion at m/z 315.0864, attributed to dihydroxy–dimethoxyflavone. In this case, the MS2 data analysis revealed a fragment at m/z 300.0733, corresponding to the loss of a methyl group. Several authors [28,29,30] have reported the presence of this compound among flavonoids found in the same genus. Compound 16 gave an [M + H]+ ion at m/z 345.0969, attributed to dihydroxy–trimethoxyflavone. The analysis revealed a fragment at m/z 330.0630, corresponding to the loss of the methyl group. The compound was reported among flavonoids found in methanolic extracts of the same species [19].
The base peak at m/z 451.1380, observed only in the methanolic extract (Rt 13.15), was attributed to the presence of 1-[9-(6-acetyl-5-hydroxy-2-benzofuranyl)-6,7,8,9-tetrahydro-2,6-dihydroxy-6-(hydroxymethyl)-3-dibenzofuranyl]-ethenone. The MS2 fragment with an m/z 402.3573 was consistent with the loss of H2O and CH2OH groups. The compound was previously reported in the Asteraceae family [31].

2.2. Antibacterial Activity

The minimum inhibitory concentration (MIC) was determined by selecting the lowest concentration of both aqueous and methanolic extracts that completely inhibited the growth of the tested pathogens in 96-well plates. The MICs of both extracts ranged from 30 to 36 µg/mL, whereas the MIC of the reference control antibiotic, tetracycline, ranged from 25 to 32 μg/mL (Table 2). The analysis revealed significant bacterial activities of both extracts against various Gram-positive (Listeria monocytogenes, Staphylococcus aureus) and Gram-negative pathogenic bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa).
A similar finding was previously observed in P. crispa extracts [32]. In fact, the same authors reported that hexane fractions from P. crispa had antibacterial activities against four bacterial pathogens (S. aureus, Klebsiella pneumoniae, E. coli, and P. aeruginosa), with MIC values ranging from 62.5 to 125 μg/mL, and were able to affect influenza A virus at various stages of its lifecycle. However, another investigation revealed that P. undulata exhibited high activity against Gram-positive bacteria in comparison with Gram-negative bacteria with mean growth inhibition zones ranging from 11.5 ± 0.2 mm against methicillin-resistant S. aureus to 21.6 ± 0.1 mm for Staphylococcus saprophyticus ATCC 43867 [2].
Furthermore, P. gnaphalodes (Vent.) Boiss. has been shown to induce substantial antibacterial activity against E. coli, Bacillus subtilis, S. aureus, and Pasteurella multocida [33]. Interestingly, both fractions in the current study had identical MIC values of 30 ± 3 µg/mL and 35 ± 3 µg/mL when tested against A. baumannii and Listeria monocytogenes, respectively, and a similar MIC value was recorded for P. aeruginosa. The methanolic extract displayed the highest antibacterial activity against E. coli, with an MIC value of 32 ± 4 µg/mL.
Overall, the MIC values of both extracts exhibited similar potency against the corresponding bacteria and were comparable to the reference control antibiotic.

2.3. Antibiofilm Activity

The effects of the aqueous and methanolic extracts against mature biofilm were evaluated at the concentrations of 10 and 20 µg/mL, using the crystal violet biofilm test. The methanolic extract at 20 µg/mL induced the highest percentage of biofilm inhibition in P. aeruginosa (55.78%), followed by S. aureus and E. coli (51.2 and 44.62%, respectively) (Table 3). The extracts showed less activity against L. monocytogenes and A. baumannii. The inhibition correlated with an increase in their concentration. A recent publication indicated that P. crispa Sch. Bip. extracts induced various potencies on bacterial detachment from polystyrene surfaces [32]. It has been suggested that the interaction of the extracts is dependent on bacterial metabolism.
The antibiofilm activity of both extracts was also assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Table 4). This test was used to evaluate the effects of the two extracts, tested at the same concentrations on the metabolism of the cells included in the biofilm niches. Both the aqueous and methanolic extracts were unable to affect the metabolism of the cells of A. baumannii, E. coli, L. monocytogenes, and P. aeruginosa included in the biofilm, at both concentrations. Conversely, the extracts were significantly active in inhibiting the metabolism of S. aureus, and the highest inhibition was observed at the highest concentration of the aqueous extract (Table 4). Finally, the methanolic extract acted against the metabolism of P. aeruginosa at the highest concentration used. The obtained results confirmed the potential antibiofilm activity of species from the Pulicaria genus; in fact, a previous study demonstrated that P. crispa extract also had different degrees of antibiofilm activity, in that case, against some K. pneumoniae strains [34].

2.4. Anti-Enzymatic Activities

Enzyme inhibitors have great physiological and medical significance. In this study, the possible anti-enzymatic activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), key enzymes involved in the termination of fast cholinergic transmission [35], and α-amylase and α-glucosidase, involved in the development of diabetes mellitus (DM) [36], were evaluated. The methanolic extract was more active on cholinesterase than the aqueous extract, although the activity of both extracts was lower than that of galantamine used as a control (Table 5). Phenolic compounds can interact with amino acid residues in the active sites of AChE and BChE [35,37]. Quercetin has been reported as a strong inhibitor of both enzymes, which are involved in the pathology of Alzheimer’s disease [38]. Here, the activity of the P. incisa extracts could have been due to the presence of chlorogenic acid. Another study reported that this compound inhibited key enzymes in rat brains in vitro [39].
No previous studies reported on the activity of AChE or BChE for P. incisa extracts. Other species of Pulicaria genus were studied for their activity against these enzymes: Zardi-Bergaoui and coworkers analyzed the anti-AchE activity of five caryophyllene sesquiterpenes isolated from P. vulgaris ethanolic extract showing IC50 values ranging from 11.1 µg/mL to 55.3 µg/mL [40], and de la Luz Cádiz-Gurrea and coworkers studied the anti-enzymatic activities of P. dysenterica aqueous and methanolic extracts, and their results showed that methanolic extract was the most potent inhibitor of both cholinesterase [41]. Moreover, P. diversifolia and P. stephanocarpa chloroformic extract showed an AChE inhibition of 41.2% and 61.4%, respectively, at 200 µg/mL [42].
To that concern, our two extracts showed no activity against α-amylase with an IC50 value > 10 mg/mL with respect to acarbose, used as a positive control, which showed an IC50 of 11 µg/mL. Instead, against α-glucosidase, the P. incisa aqueous extract was more active than the P. incisa methanolic extract with an IC50 of 393.9 µg/mL, also lower than acarbose, which was used as a standard. This study reports for the first time the possible activity of P. incisa extracts against enzymes involved in the development of diabetes.
The role of phenolic compounds against α-amylase and α-glucosidase activity remains unclear [43], and in fact, even if flavonoids can inhibit starch digestion by inhibiting α-amylase activity [44], on the other hand, phenolic acids are poor inhibitors of these enzymes [45]. However, few studies described these possible activities for other species of Pulicaria genus, and in all cases, the major activity reported for different extracts was against α-glucosidase. A P. dysenterica water extract was found to be more active against α-glucosidase than methanolic extract, as analyzed by de la Luz Cádiz-Gurrea and coworkers [41]. Pulicaria jaubertii alcoholic and n-hexane extracts were also shown to be more active against α-glucosidase than against α-amylase [46].

2.5. Total Polyphenol Content (TPC) and Total Flavonoid Content (TFC)

The total content of polyphenols and flavonoids of the two extracts is reported in Table 6. The methanolic extract (84.80 ± 2.8 mg GAE/g) had a higher amount of TPC than the aqueous extract (56.6 ± 2.1 mg GAE/g). There is limited information on P. incisa in the literature. However, Mohamed and coworkers [47] reported that the polyphenol content of a methanolic extract of P. incisa was 61.22 mg GAE/g, which is higher than that of our study. The aqueous extract presented a lower amount of total flavonoid content than the methanolic extract (11.24 ± 0.8 and 28.30 ± 1.2 mg QE/g, respectively). These results are in agreement with the literature data [16].

2.6. Antioxidant Activity

The antioxidant activity of the extracts was evaluated through the DPPH and ABTS assays, their ability to neutralize radicals, and the FRAP assay. These tests allowed for the evaluation of their antioxidant activity targeting different reactive oxygen species. The DPPH assay allows for the quantification of substances that are involved through their reducing power, both in the transfer of electrons and hydrogen. The FRAP assay, by contrast, evaluates the substances that are exclusively involved in the transfer of electrons. Lastly, the ABTS assay assesses the antioxidant activity of both hydrophilic and lipophilic molecules in a wide pH range.
The DPPH test showed that both extracts had antioxidant activity, with IC50 values lower than the literature data, particularly for the methanolic extract [16]. In fact, the available literature reports the antioxidant activity of P. crispa and P. petiolaris, as evaluated by DPPH test [48,49].
The data obtained from the FRAP and ABTS assays confirmed the DPPH assay values. The methanolic extract had higher antioxidant activity in both tests. According to the FRAP results, the methanolic extract had a higher value of mmol (Fe2+)/g than the mmol (Fe2+)/g of the aqueous extract, and also in the ABTS assay, the aqueous extract showed lower activity than the methanolic extract (Table 6). No data are available in the literature about these two assays on P. incisa, although there is information regarding other Pulicaria species. Foudah and coworkers evaluated the antioxidant activity of a methanolic extract of P. crispa by DPPH and FRAP assays [50], whereas Kozarević and coworkers analyzed P. dysenterica (L.) Gaertn. [51]. These agree with those of this study, thus highlighting the antioxidant activity of some P. incisa species. Furthermore, the antioxidant activity shown by the two extracts was closely related to the presence of polyphenols and flavonoids [52].

2.7. Computational Analysis and Interaction Assay

The computational analyses showed that P. incisa phytochemicals had various affinities to each of the targeted receptors (Table 7). Previous studies have reported that such variations are related to the chemical structures and structural geometries of ligands [53,54]. Surprisingly, all P. incisa phytochemicals possessed negative binding scores, which might have contributed to their biological activities. These binding affinities varied between −10.4 and −6.1 kcal/mol for 1JIJ, between −9.1 and −5.3 kcal/mol for 2XCT, and between −7.9 and −5.1 kcal/mol for 2UVO. The best binding scores were predicted for (−)-3,5-dicaffeoylquinic acid (compound 9), followed by calamenene (compound 10) and compounds 14, 9, or 16 for all the studied receptors, and thus the highest antimicrobial and antibiofilm activities (Table 8). (−)-3,5-Dicaffeoylquinic acid showed good molecular interactions with the targeted receptors, exhibiting 6–9 conventional H-bonds supported by a network of carbon H-bonds, hydrophobic bonds, and/or electrostatic bonds, which significantly contribute to the stability of the complexes [54,55]. These molecular interactions include several key residues: ASP40, LYS84, ARG88, VAL224, TYR36, GLN190, PRO222, ALA39, GLY193, LEU70, and VAL224 for 1JIJ; SER88, THR222, GLY34, VAL12, GLN54, ASP86, ARG51, ASP218, ARG51, and PRO120 for 2XCT; and SER43, NDG1173, NAG1174, ASN15, GLN59, PHE69, and ALA39 for 2UVO. Compound 9 was also deeply embedded in the pocket regions of 1JIJ, 2XCT, and 2UVO receptors at distances of 2.070, 1.917, and 2.176 Å, respectively (Figure 1 and Figure 2).
Overall, the calculated binding energies, molecular interactions, and deep embedding of P. incisa components confirm that its potential antibacterial and antibiofilm effects are thermodynamically possible. These effects have already been validated for other plants such as Teucrium polium, Sargassum sp., and Thymus musilii by in vitro approaches that confirmed the promising antimicrobial and antibiofilm effects of these medicinal plants and their components [53,56,57].

2.8. Bioavailability and Pharmacokinetics

The bioavailability and pharmacokinetic properties of P. incisa compounds are shown in Table 9. These properties are commonly screened to avoid drug failure in the advanced stages of drug development [53,54,55]. Our results showed that except for compounds 8, 9 and 10, all the other compounds meet the Lipinski rule of 5. Figure 3 reports that P. incisa phytochemicals are suitable for oral bioavailability, as assessed by the calculation of lipophilicity (lipo), molecular size (size), polarity (pola), insolubility (insolu), unsaturation (unsatu) and flexibility (flex). These properties mediate gastrointestinal (GI) absorption and blood–brain barrier (BBB) permeation as well. GI absorption and BBB permeation were used for the mapping of the egg model (Figure 4 and Figure 5). Good oral bioavailability was largely reported to be associated with the relevant biological activity of both synthesized and natural compounds [55]. Other than compounds 9 and 10, all other compounds were predicted as not being the substrate of P-glycoprotein (P-gp), which indicated the possible safe use of P. incisa compounds without any toxicological outcome. As cytochromes P450 (CYPs) play important roles in the interaction, metabolism, and excretion of drugs, the inhibition of five CYP enzymes was evaluated. These CYPs were 1A2, 2C19, 2C9, 2D6, and 3A4. Our results indicated that the first 10 compounds inhibited none of the assessed CYPs, which further indicated no metabolism and excretion disruptions. Log Kp values varied between −5.86 and −10.24, indicating low to moderate skin permeation. P. incisa compounds are also easy to synthesize as their synthetic accessibility values ranged between 1.46 and 5.32.

3. Materials and Methods

3.1. Plant Identity and Extraction Methods

Pulicaria incisa (Lam.) DC. (Figure 6) plant material was collected during the flowering stage from the Ha’il region of Saudi Arabia in February 2023 at the latitude 27°02′34.1″ N 42°06′53.6″ E. The species was identified by Prof. Ahmed Alghamdi, and a voucher specimen coded as PS 01 was deposited at the herbarium of the Biology Department (College of Science, University of Ha’il, Hail, Kingdom of Saudi Arabia). The plant material was air-dried at laboratory temperature (24 ± 2 °C) for several days and subsequently finely ground for further analysis. For the investigation, 40 g of fine powder was mixed with 400 mL of sterile distilled water or methanol in a 500 mL amber glass bottle and macerated for 72 h at lab temperature. Aqueous extracts were obtained after lyophilization, while the methanolic extract was obtained by removing the methanol through a rotary evaporator under vacuum. The obtained extracts were maintained in a refrigerator until use.

3.2. Chemical Analysis

Liquid chromatography high-resolution electrospray ionization mass spectrometry (LC-HRESIMS/MS) was used to analyze the extracts using an LTQ-XL Ion Trap mass spectrometer (Thermo Fisher Scientific, Rodano, Italy) equipped with an UltiMate 3000 HPLC (Agilent Technology, Cernusco sul Naviglio, Italy). A Kinetex Polar C18 column (100 × 3.0 mm, 100 Å, 2.6 µm) (Phenomenex, Torrance, CA, USA) was used for chromatographic separation. The injection volume was 0.5 mL/min, and the mobile phase was a mixture of A (0.1% formic acid in water, v/v) and B (0.1% formic acid in acetonitrile); a linear gradient was utilized, ranging from 5 to 60% B in 25 min, from 60 to 95% B in 10 min, and holding at 95% B for 5 min. In the positive mode, the HRMS and MSn spectra were recorded in data-dependent acquisition mode, causing fragmentation of the five strongest peaks for each scan. The ESI source conditions were the following: spray voltage of 4.8 kV; capillary voltage of 31 V; auxiliary gas of 15 (arbitrary units); sheath gas of 32; capillary temperature of 285 °C; normalized collision energy of 30; isolation width of 2.0; activation Q of 0.250; and activation duration of 30 ms. The measurement range was 150–1500 m/z.

3.3. Antibacterial Activity

To ensure sterility, the extracts and DMSO underwent ultrafiltration before their use in the study. The minimum inhibitory concentration (MIC) of both aqueous and methanolic extracts was determined using a modified version of the resazurin method developed by Sarker and Nahar [58]. A resazurin solution was prepared by dissolving 270 mg of resazurin in 40 mL of sterilized deionized water. In 96-well microtiter plates, the first row received 100 μL of samples in DMSO (1:10 v/v), while all other wells received 50 μL of Luria–Bertani broth or normal sterile solution. Serial dilutions of the extracts were performed in descending concentrations. To each well, 10 μL of the resazurin indicator solution was added. Furthermore, 30 μL of 3.3× sensitized broth and 10 μL of bacterial suspension (5 × 106 cfu/mL) were added to each well. The plates were sealed with parafilm to prevent dehydration. A column of the plate contained the broad-spectrum antibiotic tetracycline, which was previously suspended in DMSO and served as a positive control. The negative control consisted of Luria–Bertani broth containing resazurin and bacteria without any samples. The plates were incubated at 37 °C (35 °C for A. baumannii) for 24 h. Visual observation was used to assess any color changes. If the solution changed from dark purple to pink or colorless, it was recorded as a positive result. The MIC value was determined as the lowest concentration of extracts that could prevent the color change from dark purple to pink.

3.3.1. Antibiofilm Experiments

The inhibitory activity of the aqueous and methanolic extracts of P. incisa against the mature bacteria indicated above was evaluated using crystal violet and MTT tests [59].

3.3.2. Crystal Violet Assay

To evaluate the inhibitory activity of the extracts on mature biofilm, flat-bottomed 96-well microtiter plates were employed [60]. Bacterial cultures were adjusted to a 0.5 McFarland standard with fresh culture broth. Each well of the microtiter plate received 10 μL of the bacterial cultures and was incubated for 24 h at 37 °C (35 °C for A. baumannii). After removing planktonic cells, in each well, 10 or 20 μL/mL of the extracts were added. The final volume in each well was adjusted to 250 μL with varying amounts of Luria–Bertani broth. The plates were covered with parafilm tape to prevent evaporation and incubated at 37 °C (35 °C for A. baumannii) for another 24 h. After removing the planktonic cells, the sessile cells were washed twice with sterile PBS. Subsequently, the plates were left under a laminar flow hood for 10 min to fix the sessile cells and then removed after 15 min. The plates were allowed to dry, and the sessile cells were stained with 200 μL of a 2% w/v crystal violet solution per well for 20 min. The staining solution was discarded, and the plates were gently washed with sterile PBS. The bound dye was released by adding 200 μL of 20% w/v glacial acetic acid. The absorbance was measured at λ = 540 nm using a Cary Varian spectrophotometer (Cary Varian, Palo Alto, CA, USA). The biofilm inhibitory activity of the extracts was calculated as the percentage relative to the control (cells grown without the samples were considered to have 0% inhibition). Triplicate tests were performed, and average results were calculated for reproducibility.

3.3.3. MTT Assay

To evaluate the effect of the extracts on the metabolic activity of bacterial cells within the biofilm, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric method was employed [60]. Two concentrations of the extracts (10 and 20 μL/mL) were added after 24 h of bacterial incubation, performed as described above, after removing the planktonic cells. After another 24 h incubation, the planktonic cells were removed, and 150 μL of PBS and 30 μL of 0.3% MTT were added. The micro plates were then incubated for two hours at 37 °C (35 °C for A. baumannii). The MTT solution was removed, followed by two washing steps with 200 μL of sterile physiological solution. Finally, 200 μL of dimethyl sulfoxide (DMSO) was added to suspend the formazan crystals, and the absorbance was measured at λ = 570 nm (Cary Varian, Palo Alto, CA, USA).

3.4. Antioxidant Activity

3.4.1. DPPH Assay

The antiradical activity of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) was measured using the protocol of Xiang and coworkers [61] with slight modifications. In its radical state, DPPH possesses an absorption band at 515 nm that vanishes in the presence of antiradical chemicals. To produce final concentrations ranging from 31.25 to 1000 µg/mL, aliquots of extracts were dissolved in methanol. In a straight-sided cuvette, an aliquot of methanol solution containing different concentrations of either methanolic or aqueous extracts was added to a DPPH solution (60 μM) to a final volume of 1 mL. As a control, an identical quantity of DPPH solution was applied to the cuvette, and methanol alone was used as the blank. After 45 min, the absorbance at 515 nm was measured with a Multiskan GO spectrophotometer (Thermo Fisher Scientific, Vantaa, Finland).
The result was expressed as the IC50 value, which is the sample concentration required to lower DPPH absorbance by 50%. The standard was 6-hydroxy 2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma-Aldrich, Milan, Italy).

3.4.2. FRAP Assay

The FRAP test was carried out in accordance with the methodology of Zhang and coworkers [62,63] with the following modifications. At an acidic pH, antioxidant chemicals are tested for their capacity to decrease the complex of Fe(III)-2,4,6—tripyridyl—s—triazine (also known as [Fe (III)—(TPTZ)2]3+) to Fe (II), [Fe(II)—(TPTZ)2]2+. The resulting complex is colored (navy blue). At a wavelength of 593 nm, the reaction may be spectrophotometrically examined. The FRAP reagent was made up of 23 mM acetate buffer (pH 3.6), 10 mM tripyridyl triazine (TPTZ) in 400 mM HCl, and 20 mM FeCl3. To generate the calibration curve, several quantities of ferrous sulfate heptahydrate, FeSO4 7H2O, ranging from 27.801 mg/L to 278.010 mg/L, were made. The reaction took place in a well with a final volume of 272 µL. For 30 min, the reaction mixture was incubated in a dark environment at 37 °C. The absorbance of the FRAP alone blank was subtracted from the absorbance of the FRAP with the samples. The FeSO4 7H2O calibration curve [64] was used to calculate the FRAP values, which were reported as the mol Fe2+/g of extract. The reference standard was 6-hydroxy 2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma-Aldrich, Milan, Italy).

3.4.3. ABTS Test

The ABTS test was performed using the method described by Xiang and coworkers [61]. In ultrapure water, we made a solution of ABTS 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (7 mM) and ammonium persulfate (2.45 mM) (Sigma-Aldrich, Milan, Italy). To create the ABTS radical (ABTS•), ammonium persulfate was added to the ABTS solution until the final ammonium persulfate concentration was 2.45 mM. The sample was incubated at room temperature in the dark for 12–16 h. At 734 nm, the concentration of the ABTS radical (ABTS•) stock solution was determined to have an absorbance of 0.700 using a Multiskan GO spectrophotometer (Thermo Fisher Scientific, Vantaa, Finland). 6-hydroxy 2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma-Aldrich, Milan, Italy) was utilized as an antioxidant standard. Trolox (2.5 mM) was produced and used as a stock standard in methanol. The working standards were generated and diluted with methanol on a regular basis. In triplicate, 10 μL of the standard solution or samples and 190 μL ABTS• were added to the wells for analysis. Amounts of 10 μL of PBS and 190 μL of ultrapure water were added to the wells for the control (0 mM Trolox). The results are presented as milligrams of Trolox equivalent (TE) per gram of extract.

3.5. Analysis of Total Phenolic and Flavonoid Compounds

The total phenolic content (TPC) of the extracts was determined by spectrophotometry using the Folin–Ciocalteau technique [64]. In the cuvettes, 10 μL of diluted extract or gallic acid standard solution, 790 μL of deionized water, and 50 μL of the Folin–Ciocalteau reagent were inserted. One hundred and fifty microliters of Na2CO3 was added after 8 min. The absorbance values of the combination were measured at 765 nm after 2 h of incubation at room temperature using a Multiskan GO spectrophotometer (Thermo Fisher Scientific, Vantaa, Finland). The standard of the calibration curve was determined using gallic acid. The total phenolic content was measured in milligrams of gallic acid equivalent (mg GAE) per gram of the extract.
The total flavonoid content (TFC) was determined spectrophotometrically using the aluminum chloride colorimetric technique, as described by Baba and Malik [65], with modifications. Fifty microliters of the diluted extract or quercetin standard solution and 30 μL of 5% NaNO2 were added into a cuvette and incubated for 5 min. After the incubation, 30 μL of 10% AlCl3 was added and kept at room temperature for 5 min, then 200 μL of NaOH (1M) was supplemented to each sample and the volume was brought up to 1 mL with water; subsequently, the samples were read using a spectrophotometer at 510 nm. To make the calibration curve, quercetin was applied as a reference and the total flavonoid concentration was represented as mg quercetin equivalent (mgQE)/g of the extract.

3.6. Anti-Enzymatic Activities

3.6.1. Cholinesterase Inhibition

Cholinesterase inhibition was assessed using the method of Zheng and coworkers [66], with minor changes. In a total volume of 1 mL, 415 μL of Tris–HCl buffer 0.1 M (pH 8), 10 μL of the extract buffer solution (in 0.1% DMSO) at various concentrations (10, 5, 2.5, 1, and 0.5 mg/mL), and 25 μL of solution containing 0.28 U/mL of AChE (or BChE) were incubated for 15 min at 37 °C. The mixture was then incubated for 30 min at 37 °C with a 1.83 mM (75 μL) solution of AChI (or BChI) and 475 μL of DTNB (5,5′-dithiobis (2-nitrobenzoic acid)) and subsequently, the absorbance at 405 nm was read using a spectrophotometer (Thermo Fisher Scientific, Vantaa, Finland). Galantamine was used as a positive control.

3.6.2. α-Amylase Inhibition Assay

Jaradat’s approach, with minor modifications, was used to assess the amylase activity [67]. One hundred microliters of the extracts at various concentrations were mixed with 200 μL of 20 mM sodium phosphate buffer (pH = 6.9) and 100 μL of amylase solution (10 U/mL). For 10 min, the mixture was incubated at 37 °C. Then, 180 μL of 1% soluble starch solution was added and incubated for 20 min at 37 °C. A total of 180 μL of DNSA (3,5-dinitrosalicylic acid) solution was added to the mixture and heated for 10 min in a block heater set to 100 °C. The samples were cooled by adding 600 μL of distilled water. A UV spectrophotometer (Thermo Fisher Scientific, Vantaa, Finland) was used to measure the absorbance of the solution at 540 nm.

3.6.3. α-Glucosidase Inhibition Assay

α-Glucosidase inhibitory activity was determined as previously described [68] with minor modifications. In brief, 150 μL of 0.1 M phosphate buffer at pH 7.0 was added to each well and then 10 mg of the extracts dissolved in methanol to produce varied concentrations was added. The reaction was originated by adding 15 μL of the α-glucosidase enzyme water solution (1 U/mL) to each well in the 96-well plate and incubating at 37 °C for 5 min. Then, 75 μL of the 4-nitrophenyl-D-glucopyranoside (2.0 mM) was added and the plate was incubated for 10 min at 37 °C followed by absorbance reading at 405 nm. Acarbose and samples in phosphate buffer were used as the positive and negative controls, respectively. The results were calculated based on the equation below and the final results were presented as IC50 values.
% = [(A0 − A1)/A0] × 100
where A0 represents the absorbance of the control without the sample and A1 represents the absorbance of the sample. Sample concentration producing 50% inhibition (IC50) was gained by calculating the inhibition % against sample concentrations.

3.7. Computational Analysis and Interaction Assay

The phytochemical compounds in P. incisa that had been identified were used in the in silico approach to decipher their molecular interactions with some receptors linked with antimicrobial and antibiofilm effects. The 3D chemical structures of these compounds were either retrieved from the PubChem website or drawn using the ChemDraw software package Pro 12.0. The 3D crystal structure of TyrRS from S. aureus (1JIJ), secreted aspartic proteinase 1 from Candida albicans (2QZW), and wheat germ agglutinin (2UVO) receptors were obtained from the Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB). The studied ligands and three targeted receptors were prepared, processed for minimization, and saved in a pdbqt format [55,69]. They were subjected to a CHARMM force field as previously reported after targeting the grid box by selecting some key residues within the pocket region [53,54,55].

Bioavailability and Pharmacokinetics

Both the bioavailability and pharmacokinetic parameters of the seventeen phytochemical compounds identified in P. incisa were assessed by computational approach as previously described [54,55,70]. The analytical assessment was based on the ADMET (for absorption, distribution, metabolism, excretion and toxicity) measurements [53,55,56].

3.8. Statistical Analysis

All experiments were conducted in triplicate. Data collected from the antioxidant and anti-enzymatic assays were analyzed using SPSS 26 statistical software with one-way ANOVA followed by Tukey’s post hoc test. The differences between individual means were considered significant at p < 0.05. The data on the antibacterial activities were analyzed using GraphPad Prism 6.0 software (GraphPad Software Inc., San Diego, CA, USA) with two-way ANOVA followed by Dunnett’s multiple comparisons test.

4. Conclusions

Overall, the current study showed that P. incisa contains diverse groups of phytochemicals that display substantial antibacterial activities against several Gram-positive and Gram-negative bacteria. This potency could be a result of the capability of the bioactive compounds to target specific macromolecules within bacterial cells or external parts, such as cell walls and membranes. This investigation also demonstrated that P. incisa has great antibiofilm and antioxidant potency. The outcomes of the molecular docking analysis indicated compounds with potential antibacterial and antibiofilm activities. Moreover, the methanolic extract was shown to be active against cholinesterases and the aqueous extract, against α-glucosidase. These results comprise the first experimental data showing that P. incisa extracts could be used as adjuvants in the treatment of infections, diabetes, and neurodegenerative diseases.

Author Contributions

Conceptualization, M.A., M.S. and V.D.F.; data curation, R.B. and M.S.; formal analysis, M.A. and C.F.; investigation, M.A., M.A.A., R.B., G.A., L.C., L.D.M., F.N., F.F. and C.F.; methodology, M.A.A., L.D.M. and M.S.; supervision, M.S. and V.D.F.; validation, M.A.A., R.B., F.N. and F.F.; writing—original draft, G.A., L.C. and L.D.M.; writing—review and editing, M.A. and V.D.F. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Data Availability Statement

The data presented in this study are available upon request from the corresponding author.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Mossa, J.S.; Hifnawy, M.S.; Al-Yahya, M.A.; Al-Meshal, I.A.; Mekkawi, A.G. Aromatic Plants of Saudi Arabia-Part 8- GC/MS Analysis of Essential Oils of Pulicaria arabica and P. undulata. Int. J. Crude Drug Res. 1987, 25, 113–119. [Google Scholar] [CrossRef]
  2. Mohammed, H.A.; Al-Omar, M.S.; Khan, R.A.; Mohammed, S.A.A.; Qureshi, K.A.; Abbas, M.M.; Al Rugaie, O.; Abd-Elmoniem, E.; Ahmad, A.M.; Kandil, Y.I. Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert. Plants 2021, 10, 1811. [Google Scholar] [CrossRef]
  3. Meng, X.; Zhou, J.; Sui, N. Mechanisms of Salt Tolerance in Halophytes: Current Understanding and Recent Advances. Open Life Sci. 2018, 13, 149–154. [Google Scholar] [CrossRef]
  4. Mohammed, H.A.; Emwas, A.-H.; Khan, R.A. Salt-Tolerant Plants, Halophytes, as Renewable Natural Resources for Cancer Prevention and Treatment: Roles of Phenolics and Flavonoids in Immunomodulation and Suppression of Oxidative Stress towards Cancer Management. Int. J. Mol. Sci. 2023, 24, 5171. [Google Scholar] [CrossRef]
  5. Mohamed, E.A.A.; Muddathir, A.M.; Osman, M.A. Antimicrobial activity, phytochemical screening of crude extracts, and essential oils constituents of two Pulicaria spp. growing in Sudan. Sci. Rep. 2020, 10, 17148. [Google Scholar] [CrossRef]
  6. Mohammed, H.A.; Khan, R.A.; Abdel-Hafez, A.A.; Abdel-Aziz, M.; Ahmed, E.; Enany, S.; Mahgoub, S.; Al-Rugaie, O.; Alsharidah, M.; Aly, M.S.A.; et al. Phytochemical Profiling, In Vitro and In Silico Anti-Microbial and Anti-Cancer Activity Evaluations and Staph GyraseB and h-TOP-IIβ Receptor-Docking Studies of Major Constituents of Zygophyllum coccineum L. Aqueous-Ethanolic Extract and Its Subsequent Fractions: An Approach to Validate Traditional Phytomedicinal Knowledge. Molecules 2021, 26, 577. [Google Scholar] [CrossRef] [PubMed]
  7. Elmann, A.; Telerman, A.; Mordechay, S.; Erlank, H.; Ofir, R. Antioxidant and astroprotective effects of a Pulicaria incisa infusion. Oxidative Med. Cell. Longev. 2012, 2012, 157598. [Google Scholar] [CrossRef]
  8. Chaib, F.; Allali, H.; Bennaceur, M.; Flamini, G. Chemical Composition and Antimicrobial Activity of Essential Oils from the Aerial Parts of Asteriscus graveolens (Forssk.) Less. and Pulicaria incisa (Lam.) DC.: Two Asteraceae Herbs Growing Wild in the Hoggar. Chem. Biodivers. 2017, 14, e1700092. [Google Scholar]
  9. Bakr, R.O.; Shahat, A.; Elissawy, A.E.; Ahmed, M.; Fayez, M.; Omayma, A. Eldahshan Evaluation of the hepatoprotective activity of Pulicaria incisa subspecies candolleana and in silico screening of its isolated phenolics. J. Ethnopharmacol. 2021, 271, 113767. [Google Scholar] [CrossRef] [PubMed]
  10. Shahat, E.A.; Bakr, R.O.; Eldahshan, O.A.; Ayoub, N.A. Chemical Composition and Biological Activities of the Essential Oil from Leaves and Flowers of Pulicaria incisa sub. candolleana (Family Asteraceae). Chem. Biodivers. 2017, 14, e1600156. [Google Scholar]
  11. Pecio, Ł.; Otify, A.M.; Saber, F.R.; El-Amier, Y.A.; Shalaby, M.E.; Kozachok, S.; Elmotayam, A.K.; Świątek, Ł.; Skiba, A.; Skalicka-Woźniak, K. Iphiona mucronata (Forssk.) Asch. & Schweinf. A Comprehensive Phytochemical Study via UPLC-Q-TOF-MS in the Context of the Embryo- and Cytotoxicity Profiles. Molecules 2022, 27, 7529. [Google Scholar] [CrossRef]
  12. Erenko, E.; Mazulin, O.V.; Smoylovska, G.P.; Grechana, O.V.; Mazulin, G.V. Aminoacid composition of species of Inula L. genus of Ukraine Flora. Farmatsevtychnyi Zhurnal 2012, 3, 94–98. [Google Scholar]
  13. Mohammed, H.A.; Almahmoud, S.A.; El-Ghaly, E.-S.M.; Khan, F.A.; Emwas, A.-H.; Jaremko, M.; Almulhim, F.; Khan, R.A.; Ragab, E.A. Comparative anticancer potentials of taxifolin and quercetin methylated derivatives against HCT-116 cell lines: Effects of O-methylation on taxifolin and quercetin as preliminary natural leads. ACS Omega 2022, 7, 46629–46639. [Google Scholar] [CrossRef]
  14. Yin, J.; Li, C.; Zhang, J.; Ding, H.; Han, L.; Yang, W.; Li, F.; Song, X.; Bie, S.; Yu, H.; et al. Comprehensive multicomponent characterization and quality assessment of Shuang-Huang-Lian powder injection using ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry and ultra-high-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry. Rapid Commun. Mass Spectrom. 2023, 37, e9479. [Google Scholar]
  15. Tu, Y.; Li, L.; Wang, Z.; Yang, L. Advances in analytical techniques and quality control of traditional Chinese medicine injections. J. Pharm. Anal. 2021, 206, 114353. [Google Scholar] [CrossRef] [PubMed]
  16. El-Naggar, S.A.; Abdel-Farid, I.B.; Elgebaly, H.A.; Germoush, M.O. Metabolomic profiling, antioxidant capacity and in vitro anticancer activity of some compositae plants growing in Saudi Arabia. Afr. J. Pharm. Pharmacol. 2015, 9, 764–774. [Google Scholar]
  17. Abhimannue, A.P.; Mohan, M.C. Inhibition of tumor necrosis factor-α and interleukin-1β production in lipopolysaccharide-stimulated monocytes by methanolic extract of Elephantopus scaber linn and identification of bioactive components. Appl. Biochem. Biotechnol. 2016, 179, 427–443. [Google Scholar] [CrossRef] [PubMed]
  18. Yang, J.-H.; Bai, T.-C.; Shi, L.-L.; Hou, B.; Tang, R.; Zhang, R.-P.; Chen, X.-L. Antihyperlipidemic effect of Vaccinium dunalianum buds based on biological activity screening and LC-MS. J. Ethnopharmacol. 2023, 306, 116190. [Google Scholar] [CrossRef] [PubMed]
  19. El-Sabagh, O.A.; El-Toumy, S.A.; Mounir, R.; Farag, M.A.; Mahrous, E.A. Metabolite profiles of Pulicaria crispa and P. incisa in relation to their in-vitro/in-vivo antioxidant activity and hepatoprotective effect: A comparative mass spectrometry-based metabolomics. J. Pharm. Biomed. Anal. 2021, 194, 113804. [Google Scholar] [CrossRef]
  20. Al-Naqeb, G.; Rousová, J.; Kubátová, A.; Picklo Sr, M.J. Pulicaria jaubertii E. Gamal-Eldin reduces triacylglyceride content and modifies cellular antioxidant pathways in 3T3-L1 adipocytes. Chem. Biol. Interact 2016, 253, 48–59. [Google Scholar] [CrossRef]
  21. Askari, H.; Ghaedi, M.; Naghiha, R.; Salehi, A. In Vitro antibacterial and antifungal studies of Pulicaria undulate and Echinacea purpurea extracts in combination with nanowires (Ni: FeO (OH)) and nanoparticles (NiS). Jundishapur J. Nat. Pharm. Prod. 2020, 15, e64358. [Google Scholar] [CrossRef]
  22. Wang, Y.H.; Al-Rehaily, A.J.; Yousaf, M.; Ahmad, M.S.; Khan, I.A. Characterization and Discrimination of Different Pulicaria Species Using UHPLC-UV-MS QTOF (Quadrupole Time-of-Flight Mass Spectrometer). J. Chem. Soc. Pak. 2015, 37, 967–973. [Google Scholar]
  23. Ibraheim, Z.; Darwish, F. Further Constituents from Pulicaria Incisa. Bull. Fac. Pharm. 2002, 40, 167–173. [Google Scholar]
  24. Mohti, H.; Taviano, M.F.; Cacciola, F.; Dugo, P.; Mondello, L.; Marino, A.; Crisafi, G.; Benameur, Q.; Zaid, A.; Miceli, N. Inula viscosa (L.) Aiton leaves and flower buds: Effect of extraction solvent/technique on their antioxidant ability, antimicrobial properties and phenolic profile. Nat. Prod. Res. 2020, 34, 46–52. [Google Scholar] [CrossRef] [PubMed]
  25. Meng, J.; Zhang, Y.; Wang, G.; Ji, M.; Wang, B.; He, G.; Wang, Q.; Bai, F.; Xu, K.; Yuan, D.; et al. Conduction of a chemical structure-guided metabolic phenotype analysis method targeting phenylpropane pathway via LC-MS: Ginkgo biloba and soybean as examples. Food Chem. 2022, 390, 133155. [Google Scholar] [CrossRef] [PubMed]
  26. Zhao, H.; Zhu, M.; Wang, K.; Yang, E.; Su, J.; Wang, Q.; Cheng, N.; Xue, X.; Wu, L.; Cao, W. Identification and quantitation of bioactive components from honeycomb (Nidus Vespae). Food Chem. 2020, 314, 126052. [Google Scholar] [CrossRef]
  27. Waridel, P.; Wolfender, J.-L.; Lachavanne, J.-B.; Hostettmann, K. Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside. Phytochemistry 2004, 65, 2401–2410. [Google Scholar] [CrossRef]
  28. Ibraheim, Z.Z.; Salem, H.A. Phytochemical and pharmacological studies on Pulicaria orientalis Jaub & Sp. Bull. Pharm. Sci. 2002, 25, 189–200. [Google Scholar]
  29. Williams, C.A.; Harborne, J.B.; Greenham, J.R.; Grayer, R.J.; Kite, G.C.; Eagles, J. Variations in lipophilic and vacuolar flavonoids among European Pulicaria species. Phytochemistry 2003, 64, 275–283. [Google Scholar] [CrossRef] [PubMed]
  30. Wollenweber, E.; Christ, M.; Dunstan, R.H.; Roitman, J.N.; Stevens, J.F. Exudate flavonoids in some Gnaphalieae and Inuleae (Asteraceae). Z. Naturforschung 2005, 60, 671–678. [Google Scholar] [CrossRef]
  31. Xiao, L.; Huang, Y.; Wang, Y.; Xu, J.; He, X. Anti-neuroinflammatory benzofurans and lignans from Praxelis clematidea. Fitoterapia 2020, 140, 104440. [Google Scholar] [CrossRef] [PubMed]
  32. Abo-Elghiet, F.; Rushdi, A.; Ibrahim, M.H.; Mahmoud, S.H.; Rabeh, M.A.; Alshehri, S.A.; El Menofy, N.G. Chemical Profile, Antibacterial, Antibiofilm, and Antiviral Activities of Pulicaria crispa Most Potent Fraction: An In Vitro and In Silico Study. Molecules 2023, 28, 4184. [Google Scholar] [CrossRef]
  33. Naqvi, S.A.R.; Shah, S.M.A.; Kanwal, L.; Saeed, M.; Ul-Haq, A.; Nisar, J.; Nisar, Z.; Akram, M. Antimicrobial and Antihypercholesterolemic Activities of Pulicaria gnaphalodes. Dose-Response 2020, 18, 1–6. [Google Scholar] [CrossRef]
  34. Thinina, A.C.; Karim, H.; Alia, M.M.; Karim, A. Evaluation and quantification of the inhibition of biofilm and planktonic forms of Klebsiella pneumoniae by the polyphenolic extract of Pulicaria crispa. J. Adv. Pharm. Technol. Res. 2020, 11, 117–122. [Google Scholar] [CrossRef] [PubMed]
  35. Jabir, N.R.; Khan, F.R.; Tabrez, S. Cholinesterase targeting by polyphenols: A therapeutic approach for the treatment of Alzheimer’s disease. CNS Neurosci. Ther. 2018, 24, 753–762. [Google Scholar] [CrossRef] [PubMed]
  36. Etsassala, N.G.E.R.; Badmus, J.A.; Marnewick, J.L.; Iwuoha, E.I.; Nchu, F.; Hussein, A.A. Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities, Molecular Docking, and Antioxidant Capacities of Salvia aurita Constituents. Antioxidants 2020, 9, 1149. [Google Scholar] [CrossRef]
  37. Katalinić, M.; Rusak, G.; Barović, J.D.; Šinko, G.; Jelić, D.; Antolović, R.; Kovarik, Z. Structural aspects of flavonoids as inhibitors of human butyrylcholinesterase. Eur. J. Med. Chem. 2010, 45, 186–192. [Google Scholar] [CrossRef]
  38. Calderaro, A.; Patanè, G.T.; Tellone, E.; Barreca, D.; Ficarra, S.; Misiti, F.; Laganà, G. The Neuroprotective Potentiality of Flavonoids on Alzheimer’s Disease. Int. J. Mol. Sci. 2022, 23, 14835. [Google Scholar] [CrossRef] [PubMed]
  39. Oboh, G.; Agunloye, O.M.; Adefegha, S.A.; Akinyemi, A.J.; Ademiluyi, A.O. Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): A comparative study. J. Basic Clin. Physiol. Pharmacol. 2015, 26, 165–170. [Google Scholar] [CrossRef]
  40. Zardi-Bergaoui, A.; Znati, M.; Harzallah-Skhiri, F.; Jannet, H.B. Caryophyllene sequiterpenes from Pulicaria vulgaris Gaertn.: Isolation, Structure Determination, Bioactivity and Structure—Activity Relationship. Chem. Biodivers. 2019, 16, e1800483. [Google Scholar] [CrossRef]
  41. de la Luz Cádiz-Gurrea, M.; Zengin, G.; Kayacık, O.; Lobine, D.; Mahomoodally, M.F.; Leyva-Jiménez, F.J.; Segura-Carretero, A. Innovative perspectives on Pulicaria dysenterica extracts: Phyto-pharmaceutical properties, chemical characterization and multivariate analysis. J. Sci. Food Agric. 2019, 99, 6001–6010. [Google Scholar] [CrossRef]
  42. Bakthir, H.; Ali, N.A.; Arnold, N.; Teichert, A.; Wessjohann, L. Anticholinesterase activity of endemic plant extracts from Soqotra. Afr. J. Tradit. Complement. Altern. Med. 2011, 8, 296–299. [Google Scholar] [CrossRef] [PubMed]
  43. Giuberti, G.; Rocchetti, G.; Lucini, L. Interactions between phenolic compounds, amylolytic enzymes and starch: An updated overview. Curr. Opin. Food Sci. 2020, 31, 102–113. [Google Scholar] [CrossRef]
  44. Takahama, U.; Hirota, S. Interactions of flavonoids with α-amylase and starch slowing down its digestion. Food Funct. 2018, 9, 677–687. [Google Scholar] [CrossRef] [PubMed]
  45. Nyambe-Silavwe, H.; Williamson, G. Chlorogenic and phenolic acids are only very weak inhibitors of human salivary α-amylase and rat intestinal maltase activities. Food Res. Int. 2018, 113, 452–455. [Google Scholar] [CrossRef] [PubMed]
  46. Mohammed, H.A.; Abdelwahab, M.F.; El-Ghaly, E.S.M.; Ragab, E.A. Phytochemical Characterization, In Vitro Anti-Inflammatory, Anti-Diabetic, and Cytotoxic Activities of the Edible Aromatic Plant; Pulicaria jaubertii. Molecules 2021, 26, 203. [Google Scholar] [CrossRef]
  47. Mohamed, M.S.; Saleh, A.M.; Abdel-Farid, I.B.; El-Naggar, S.A. Growth, hydrolases and ultrastructure of Fusarium oxysporum as affected by phenolic rich extracts from several xerophytic plants. Pestic. Biochem. Physiol. 2017, 141, 57–64. [Google Scholar] [CrossRef]
  48. Marwah, R.G.; Fatope, M.O.; Al Mahrooqi, R.; Varma, G.B.; Al Abadi, H.; Al-Burtamani, S.K.S. Antioxidant capacity of some edible and wound healing plants in Oman. Food Chem. 2007, 101, 465–470. [Google Scholar] [CrossRef]
  49. Mothana, R.A.; Kriegisch, S.; Harms, M.; Wende, K.; Lindequist, U. Assessment of selected Yemeni medicinal plants for their in vitro antimicrobial, anticancer, and antioxidant activities. Pharm. Biol. 2011, 49, 200–210. [Google Scholar] [CrossRef]
  50. Foudah, A.I.; Alam, A.; Soliman, G.A.; Salkini, M.A.; Ahmed, E.I.; Yusufoglu, H.S. Pharmacognostical, antioxidant and antimicrobial studies of aerial part of Pulicaria crispa (Family: Asteraceae). Bull. Environ. Pharmacol. Life Sci. 2015, 4, 19–27. [Google Scholar]
  51. Kozarević Cilović, E.; Dautović, E.; Halilčević, D.; Softić, A.; Srabović, N.; Šarić-Kundalić, B.; Delic, N.; Kolarevic, L.; Mekić, L.; Ibišević, M.; et al. Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. Int. Res. J. Pure Appl. Chem. 2022, 23, 23–32. [Google Scholar] [CrossRef]
  52. Farhat, M.B.; Chaouch-Hamada, R.; Sotomayor, J.A.; Landoulsi, A.; Jordán, M.J. Antioxidant potential of Salvia officinalis L. residues as affected by the harvesting time. Ind. Crops Prod. 2014, 54, 78–85. [Google Scholar] [CrossRef]
  53. Rahmouni, F.; Badraoui, R.; Ben-Nasr, H.; Bardakci, F.; Elkahoui, S.; Siddiqui, A.J.; Saeed, M.; Snoussi, M.; Saoudi, M.; Rebai, T. Pharmacokinetics and therapeutic potential of Teucrium polium against liver damage associated hepatotoxicity and oxidative injury in rats: Computational, biochemical and histological studies. Life 2022, 12, 1092. [Google Scholar] [CrossRef]
  54. Mhadhbi, N.; Issaoui, N.; Hamadou, W.S.; Alam, J.M.; Elhadi, A.S.; Adnan, M.; Nahili, H.; Badraoui, R. Physico-Chemical Properties, Pharmacokinetics, Molecular Docking and In-Vitro Pharmacological Study of a Cobalt (II) Complex Based on 2-Aminopyridine. ChemistrySelect 2022, 7, e20210359. [Google Scholar] [CrossRef]
  55. Badraoui, R.; Saoudi, M.; Hamadou, W.S.; Elkahoui, S.; Siddiqui, A.J.; Alam, J.M.; Jamal, A.; Adnan, M.; Suliemen, A.M.E.; Alreshidi, M.M.; et al. Antiviral Effects of Artemisinin and Its Derivatives against SARS-CoV-2 Main Protease: Computational Evidences and Interactions with ACE2 Allelic Variants. Pharmaceuticals 2022, 15, 129. [Google Scholar] [CrossRef]
  56. Alreshidi, M.; Badraoui, R.; Adnan, M.; Patel, M.; Alotaibi, A.; Saeed, M.; Ghandourah, M.; Al-Motair, K.A.; Arif, I.A.; Albulaihed, Y.; et al. Phytochemical profiling, antibacterial, and antibiofilm activities of Sargassum sp. (brown algae) from the Red Sea: ADMET prediction and molecular docking analysis. Algal Res. 2023, 69, 102912. [Google Scholar]
  57. Noumi, E.; Ahmad, I.; Bouali, N.; Patel, H.; Ghannay, S.; ALrashidi, A.A.; Abdulhakeem, M.A.; Patel, M.; Ceylan, O.; Badraoui, R.; et al. Thymus musilii Velen. Methanolic Extract: In Vitro and In Silico Screening of Its Antimicrobial, Antioxidant, Anti-Quorum Sensing, Antibiofilm, and Anticancer Activities. Life 2023, 13, 62. [Google Scholar] [CrossRef] [PubMed]
  58. Sarker, S.D.; Nahar, L.; Kumarasamy, Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007, 42, 321–324. [Google Scholar] [CrossRef] [PubMed]
  59. Fratianni, F.; d’Acierno, A.; Ombra, M.N.; Amato, G.; De Feo, V.; Ayala-Zavala, J.F.; Coppola, R.; Nazzaro, F. Fatty Acid Composition, Antioxidant, and in vitro Anti-inflammatory Activity of Five Cold-Pressed Prunus Seed Oils, and Their Anti-biofilm Effect Against Pathogenic Bacteria. Front. Nutr. 2021, 8, 775751. [Google Scholar] [CrossRef] [PubMed]
  60. Nazzaro, F.; Polito, F.; Amato, G.; Caputo, L.; Francolino, R.; d’Acierno, A.; Fratianni, F.; Candido, V.; Coppola, R.; De Feo, V. Chemical composition of Essential Oils of bulbs and aerial parts of two cultivars of Allium sativum and their antibiofilm activity against food and nosocomial pathogens. Antibiotics 2022, 11, 724. [Google Scholar] [CrossRef]
  61. Xiang, J.; Apea-Bah, F.B.; Ndolo, V.U.; Katundu, M.C.; Beta, T. Profile of phenolic compounds and antioxidant activity of finger millet varieties. Food Chem. 2019, 275, 361–368. [Google Scholar] [CrossRef] [PubMed]
  62. Zhang, M.; Xu, Y.; Xiang, J.; Zheng, B.; Yuan, Y.; Luo, D.; Fan, J. Comparative evaluation on phenolic profiles, antioxidant properties and α-glucosidase inhibitory effects of different milling fractions of foxtail millet. J. Cereal Sci. 2021, 99, e103217. [Google Scholar] [CrossRef]
  63. Amamcharla, J.K.; Metzger, L.E. Modification of the ferric reducing antioxidant power (FRAP) assay to determine the susceptibility of raw milk to oxidation. Int. Dairy J. 2014, 34, 177–179. [Google Scholar] [CrossRef]
  64. Waterhouse, A.L. Determination of total phenolics. Curr. Protoc. Food Anal. Chem. 2002, 6, 1–8. [Google Scholar]
  65. Baba, S.A.; Malik, S.A. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. J. Taibah Univ. Sci. 2015, 9, 449–454. [Google Scholar] [CrossRef]
  66. Zheng, B.; Yuan, Y.; Xiang, J.; Jin, W.; Johnson, J.B.; Li, Z.; Wang, C.; Luo, D. Green extraction of phenolic compounds from foxtail millet bran by ultrasonic-assisted deep eutectic solvent extraction: Optimization, comparison and bioactivities. LWT 2022, 154, 112740. [Google Scholar] [CrossRef]
  67. Jaradat, N.; Al-Maharik, N.; Abdallah, S.; Shawahna, R.; Mousa, A.; Qtishat, A. Nepeta curviflora essential oil: Phytochemical composition, antioxidant, anti-proliferative and anti-migratory efficacy against cervical cancer cells, and α-glucosidase, α-amylase and porcine pancreatic lipase inhibitory activities. Ind. Crops Prod. 2020, 158, 112946. [Google Scholar] [CrossRef]
  68. Nguyen, T.K.; Tran, L.T.T.; Viet, D.H.; Thai, P.H.; Ha, T.P.; Ty, P.V.; Huu, D.T.T. Xanthine oxidase, α-glucosidase and α-amylase inhibitory activities of the essential oil from Piper lolot: In vitro and in silico studies. Heliyon 2023, 9, e19148. [Google Scholar] [CrossRef]
  69. Akacha, A.; Badraoui, R.; Rebai, T.; Zourgui, L. Effect of Opuntia Ficus Indica Extract on Methotrexate-Induced Testicular Injury: A Biochemical, Docking and Histological Study. J. Biomol. Struct. Dyn. 2020, 40, 4341–4351. [Google Scholar] [CrossRef]
  70. Badraoui, R.; Mannai, G.; Siddiqui, A.J.; Pacioglu, O.; Rudayni, H.A.; Boufahja, F.; Essid, N. How toxic is the COVID-19 drug azithromycin in the presence of Posidonia oceanica? Toxicokinetics and experimental approach of meiobenthic nematodes from a metallically pristine area. Environ. Pollut. 2023, 319, 121007. [Google Scholar] [CrossRef] [PubMed]
Molecules 28 07439 g001
Figure 1. LC-MS profiles (base peak chromatogram) in positive ion mode of Pulicaria incisa (A) methanolic and (B) aqueous extracts.
Figure 1. LC-MS profiles (base peak chromatogram) in positive ion mode of Pulicaria incisa (A) methanolic and (B) aqueous extracts.
Molecules 28 07439 g001
Molecules 28 07439 g002
Figure 2. 3D illustration of the H-bond interactions of (−)-3,5-dicaffeoylquinic acid (Compound No. 9) of P. incisa that had the best binding affinity docket to the pocket region of the different targeted receptors: (A) 1JIJ, (B) 2XCT, and (C) 2UVO.
Figure 2. 3D illustration of the H-bond interactions of (−)-3,5-dicaffeoylquinic acid (Compound No. 9) of P. incisa that had the best binding affinity docket to the pocket region of the different targeted receptors: (A) 1JIJ, (B) 2XCT, and (C) 2UVO.
Molecules 28 07439 g002
Molecules 28 07439 g003
Figure 3. 2D illustration of the diagram of interactions of (−)-3,5-dicaffeoylquinic acid (Compound No. 9) of P. incisa that had the best binding affinity docket to the different targeted receptors: (A) 1JIJ, (B) 2XCT, and (C) 2UVO.
Figure 3. 2D illustration of the diagram of interactions of (−)-3,5-dicaffeoylquinic acid (Compound No. 9) of P. incisa that had the best binding affinity docket to the different targeted receptors: (A) 1JIJ, (B) 2XCT, and (C) 2UVO.
Molecules 28 07439 g003
Molecules 28 07439 g004
Figure 4. Bioavailability hexagons of P. incisa L. identified compounds (1–17) based on their physicochemical properties.
Figure 4. Bioavailability hexagons of P. incisa L. identified compounds (1–17) based on their physicochemical properties.
Molecules 28 07439 g004
Molecules 28 07439 g005
Figure 5. Boiled egg model of P. incisa L. identified compounds (1–17) based on their GI absorption, BBB permeation, and interaction with P-gp properties.
Figure 5. Boiled egg model of P. incisa L. identified compounds (1–17) based on their GI absorption, BBB permeation, and interaction with P-gp properties.
Molecules 28 07439 g005
Molecules 28 07439 g006
Figure 6. Pulicaria incisa collected from the Ha’il region ((A): Whole plant; (B): Flower and leaves).
Figure 6. Pulicaria incisa collected from the Ha’il region ((A): Whole plant; (B): Flower and leaves).
Molecules 28 07439 g006
Table 1. Composition of methanolic (PM) and aqueous (PW) extracts of P. incisa. Compounds were listed in order of LC-MS elution. All mass peaks are [M + H]+ adducts.
Table 1. Composition of methanolic (PM) and aqueous (PW) extracts of P. incisa. Compounds were listed in order of LC-MS elution. All mass peaks are [M + H]+ adducts.
No.FamilyRetention
Time (min)		Measured
m/z		Molecular
Formula		FragmentFragment
Formula		Fragment Ion (m/z)ΔppmIdentificationPMPW
1Amino acid
derivative		1.30294.1545C12H23O7N[M-H2O + H]+
[M-2H2O + H]+
[M-CHO2 + H]+		C12H22O6N
C12H20O5N
C11H22O5N		276.1443
258.1342
248.1494		0.674
2.521
0.527		N-Fructosyl (iso)leucineXX
2Amino acid
derivative		2.10166.0862C9H11O2N[M-NH2 + H]+
[M-CHO2 + H]+		C9H9O2
C8H10N		149.0594
120.0806		−1.919
−1.798		L-PhenylalanineXX
3Phenolic acid7.10355.1019C16H18O9[M-H2O + H]+
[M-C7H12O6 + H]+
[M-C7H12O6-CO-H2O + H]+		C16H17O8
C9H7O3
C8H5O		337.0926
163.0391
117.0335		2.362
0.855
−0.097		Chlorogenic acidXX
4α-β-Unsaturated γ-lactone7.52199.1328C11H18O3[M-H2O + H]+
[M-2H2O + H]+		C11H17O2
C11H15O		181.1221
163.1104		−1.139
−8.225		(−)-HydroxydihydrobovolideXX
5Phenolic acid7.65339.0926C16H18O8[M-C7H12O6 + H]+
[M-C7H12O6-CO + H]+		C9H7O2
C8 H7O		147.0438
119.0484		−1.469
−6.144		O-Coumaroylquinic acidXX
6Phenolic acid7.82369.1042C17H20O9[M-C7H12O6 + H]+
[M-C7H12O6-CH3O + H]+		C10H9O3
C9 H5O2		177.0545
145.0285		−0.569
0.648		Feruloylquinic acidX
7Unsaturated ketone8.12169.1222C10H16O2[M-H2O + H]+
[M-H2O-C3H6 + H]+		C10H15O
C7H9O		151.1108
109.0658		−6.496
9.522		8-HydroxycarvotanacetoneXX
8Flavonoid8.30465.1020C21H20O12[M-H2O + H]+
[M-C6H12O6 + H]+		C21H19O11
C15H11O7		447.0923
303.0497		0.363
−0.723		Isoquercetin (quercetin 3-O-β-D-glucoside)X
9Phenolic acid8.80517.1329C25H24O12[M-H2O + H]+
[M-C9H7O3 + H]+
[M-C9H7O3-C7H12O6 + H]+		C25H23O11
C16H19O9
C9H7O3		499.1230
355.1027
163.0388		−1.058
0.849
−0.740		(−)-3,5-Dicaffeoylquinic acidXX
10Sesquiterpene
hydrocarbon		9.74203.1792C15H22[M-CH(CH3)2 + H]+
[M-CH(CH3)2-CH3 + H]+		C12H17
C11H15		161.1325
147.1165
0.080
−1.883		CalameneneXX
11Flavonoid11.12317.0652C16H12O7[M-H2O + H]+
[M-C6H4O2 + H]+
[M-C6H4O2-H2O + H]+		C16H11O6
C10H9O5
C10H7O4		299.0550
209.0448
191.0336		0.018
1.771
−1.493		RhamnetinXX
12Flavonoid11.35331.0806C17H14O7[M-CH3 + H]+
[M-CH3-H2O + H]+		C16H12O7
C16H11O6		316.0573
299.0568		−1.310
6.138		Quercetin dimethyl etherXX
13Flavonoid11.77287.0912C16H14O5[M-H2O + H]+
[M-C8H6O + H]+		C16H13O4
C8H7O4		269.0801
167.0338		−2.584
−0.151		SakuranetinX
14Flavonoid11.95301.0705C16H12O6[M-CH3 + H]+C15H10O6286.07841.324ChrysoeriolXX
15Flavonoid12.25315.0864C17H14O6[M-CH3 + H]+C16H12O6300.06300.501Dihydroxy–dimethoxyflavoneXX
16Flavonoid12.32345.0969C18H16O7[M-CH3 + H]+
[M-2CH3 + H]+		C17H14 O7
C16H11O7		330.0733
315.0509		−0.316
3.177		Dihydroxy–trimethoxyflavoneXX
17Benzofuran dimer13.15451.1380C25H22O8[M-CH2OH-H2O + H]+C24H18O6402.3573−1.0571-[9-(6-Acetyl-5-hydroxy-2-benzofuranyl)-6,7,8,9-tetrahydro-2,6-dihydroxy-6-(hydroxymethyl)-3-dibenzofuranyl] ethanoneX
Table 2. Minimal inhibitory concentration (µg/mL) of aqueous and methanolic extracts against various pathogenic bacteria in comparison to reference control tetracycline.
Table 2. Minimal inhibitory concentration (µg/mL) of aqueous and methanolic extracts against various pathogenic bacteria in comparison to reference control tetracycline.
A. baumanniiE. coliL. monocytogenesP. aeruginosaS. aureus
P. incisa (Ext. H2O)35 ± 4 a35 ± 3 a35 ± 3 a33 ± 2 a36 ± 2 a
P. incisa (Ext. MeOH)35 ± 2 a30 ± 3 a35 ± 4 a32 ± 4 a32 ± 2 a
Tetracycline25 ± 225 ± 232 ± 128 ± 128 ± 1
Results are reported as the means ± SD of three experiments. Different letters indicate mean values significantly different at p < 0.05, according to a one-way ANOVA followed by Tukey’s post hoc test.
Table 3. Inhibitory activity of the extracts on the mature biofilm (24 h).
Table 3. Inhibitory activity of the extracts on the mature biofilm (24 h).
Percentage of Inhibition Compared to the Control
CV (24 h)P. incisa (Ext. H2O) 10 μg/mLP. incisa (Ext. H2O) 20 μg/mLP. incisa (Ext. MeOH) 10 μg/mLP. incisa (Ext. MeOH)
20 μg/mL
A. baumannii19.13 **** ± 1.0219.37 **** ± 1.2612.35 *** ± 0.4619.53 **** ± 1.18
E. coli4.38 * ± 0.1225.5 **** ± 1.5732.87 **** ± 1.8844.62 **** ± 2.87
L. monocytogenes14.93 **** ± 1.0217.21 **** ± 1.163.32 * ± 0.1118.10 **** ± 1.04
P. aeruginosa24.36 **** ± 1.0936.68 **** ± 1.4429.32 **** ± 2.8655.78 **** ± 2.55
S. aureus8.26 ** ± 0.1132.54 **** ± 1.4331.31 **** ± 2.9751.27 **** ± 2.07
The tests were performed using 10 and 20 μg/mL of extract. All tests were performed in triplicate. Results are expressed as percentages (means ± SD) and were calculated assuming the control (untreated bacteria) = zero. *: p < 0.1; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001, compared with the control (ANOVA followed by Dunnett’s multiple comparisons test).
Table 4. Inhibitory activity of the extracts on the metabolism of the bacterial sessile cell in the mature biofilm (24 h).
Table 4. Inhibitory activity of the extracts on the metabolism of the bacterial sessile cell in the mature biofilm (24 h).
Percentage of Inhibition Compared to the Control
MTT (24 h)P. incisa (Ext. H2O)
10 μg/mL		P. incisa (Ext. H2O) 20 μg/mLP. incisa (Ext. MeOH) 10 μg/mLP. incisa (Ext. MeOH)
20 μg/mL
A. baumannii0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
E. coli0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
L. monocytogenes0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
P. aeruginosa0.00 ± 0.0019.98 **** ± 0.000.00 ± 0.000.00 ± 0.00
S. aureus49.77 **** ± 3.8877.94 **** ± 4.0612.74 **** ± 1.0230.52 **** ± 2.84
The tests were performed using 10 and 20 μg/mL of extract. All tests were performed in triplicate. Results are expressed as inhibition percentages (means ± SD) and were calculated assuming the control (untreated bacteria) = zero. ****: p < 0.0001, compared with the control (ANOVA followed by Dunnett’s multiple comparisons test).
Table 5. Inhibitory effects of Pulicaria incisa extracts on AChE, BChE, and α-amylase and α-glucosidase.
Table 5. Inhibitory effects of Pulicaria incisa extracts on AChE, BChE, and α-amylase and α-glucosidase.
IC50 (µg/mL)
AChEBChEα-Amylaseα-Glucosidase
Pulicaria incisa (aqueous extract)1621.5 ± 109.6 b1092.5 ± 6.4 bn.a.393.9 ± 39.9 a
Pulicaria incisa (methanolic extract)292.3 ± 1.5 a236.9 ± 15.7 an.a.1936.5 ± 164.7 b
Galantamine0.9 ± 0.44.6 ± 1.5––
Acarbose––11 ± 4.2963 ± 0.8
Results are reported as the means ± SD of three experiments. Different letters indicate mean values significantly different at p < 0.05, according to a one-way ANOVA followed by Tukey’s post hoc test. n.a. = not active (IC50 > 10,000 µg/mL).
Table 6. Total polyphenol content (TPC), total flavonoid content (TFC), and antioxidant activity of Pulicaria incisa extracts.
Table 6. Total polyphenol content (TPC), total flavonoid content (TFC), and antioxidant activity of Pulicaria incisa extracts.
Antioxidant Activity
TPC
mg (GAE)/g		TFC
mg (QE)/g		DPPH (IC50) μg/mLFRAP mmol (Fe2+)/gABTS TEAC mg (TE)/g
P. incisa (ext. H2O)56.6 a ± 2.111.24 a ± 0.864.27 b ± 0.580.93 a ± 0.0984.42 a ± 7.2
P. incisa
(ext. MeOH)		84.80 b ± 2.828.30 b ± 1.223.29 a ± 0.382.99 b ± 0.14201.86 b ± 7.8
Trolox––3.65 ± 0.0110.30 a ± 0.08–
Results are reported as the means ± SD of three experiments. Different letters indicate mean values significantly different at p < 0.05, according to a one-way ANOVA followed by Tukey’s post hoc test.
Table 7. Binding affinities of identified P. incisa L. components (1–17) with three targeted receptors: 1JIJ, 2QZW, and 2UVO.
Table 7. Binding affinities of identified P. incisa L. components (1–17) with three targeted receptors: 1JIJ, 2QZW, and 2UVO.
Compound No.Binding Affinity (kcal × mol−1)
1JIJ2XCT2UVO
1−8.0−7.4−6.8
2−6.3−6.1−5.4
3−8.6−7.5−7.1
4−6.9−5.7−5.4
5−8.1−7.8−7.0
6−9.0−7.0−6.6
7−6.1−5.3−5.1
8−7.7−7.8−7.3
9−10.4−9.1−7.9
10−10.1−8.4−7.8
11−9.6−8.0−6.7
12−9.1−7.8−6.8
13−8.6−7.5−6.8
14−9.7−8.0−7.2
15−8.4−7.6−6.7
16−9.7−7.8−6.9
17−7.7−7.0−6.8
Table 8. Numbers of conventional H-bonds, closest interacting residues, and distance to closest interacting residue (Å) of the major P. incisa L. active chemical components that had the best binding affinities with the three targeted receptors: 1JIJ, 2XCT, and 2UVO.
Table 8. Numbers of conventional H-bonds, closest interacting residues, and distance to closest interacting residue (Å) of the major P. incisa L. active chemical components that had the best binding affinities with the three targeted receptors: 1JIJ, 2XCT, and 2UVO.
ReceptorChemical
Compound No.		No. Conventional
H-Bonds		Closest
Interacting Residues		Closest Residue (Distance, Å)No. Closest Interacting Residues
1JIJ99Conventional H-Bond: ASP40, LYS84, LYS84, ARG88, VAL224, TYR36, GLN190, PRO222, PRO222
Carbon H-Bond: ALA39, GLY193
Hydrophobic: LEU70, VAL224		VAL224:HN (2.070)10
1010Conventional H-Bond: LYS84, LYS84, GLY193, GLN196, VAL224, THR75, TYR170, ASP177, ASP195, PRO220
Carbon H-Bond: GLY193, ASP195		VAL224:HN (1.980)9
146Conventional H-Bond: LYS84, LYS84, LYS84, ARG88, ASP177, GLN190
Carbon H-Bond: ASP40
Electrostatic: ASP80		GLN190:OE1 (2.064)6
168Conventional H-Bond: LYS84, LYS84, LYS84, ARG88, TYR36, GLN190, THR75, ASP177
Carbon H-Bond: ASP40
Electrostatic: ASP80
Hydrophobic: HIS50, LEU70		LYS84:HZ1 (2.184)10
2XCT96Conventional H-Bond: SER88, THR222, GLY34, VAL12, THR222, GLN54
Carbon H-Bond: ASP86
Electrostatic: ARG51, ASP218
Hydrophobic: ARG51, PRO120		THR222:OG1 (1.917)9
106Conventional H-Bond: ARG51, SER88, THR222, SER301, SER301, LYS49
Carbon H-Bond: GLY220
Hydrophobic: PHE58, TYR225		SER301:HG (2.023)8
116Conventional H-Bond: ARG195, ASP214, SER190, ASP191, GLU193, ALA303
Carbon H-Bond: GLN227
Hydrophobic: ILE213		ASP214:HN (1.961)8
145Conventional H-Bond: ARG192, THR221, ASP218, THR221, ASP37
Electrostatic: ASP218
Hydrophobic: ILE305, ALA133		ASP218:OD2 (2.340)6
2UVO910Conventional H-Bond: SER43, SER43, NDG1173, NAG1174, NDG1173, NAG1174, NDG1173, NAG1174, ASN15, GLN59
Hydrophobic: PHE69, ALA39		SER43:HG (2.176)7
108Conventional H-Bond: GLY113, NAG1176, ILE155, CYS153, GOL1177, NAG1176, ASP129, CYS153
Electrostatic: ASP129		NAG1176:H3 (2.057)6
810Conventional H-Bond: NAG1176, GOL1177, GOL1177, GOL1177, NAG1176, ASP129, GOL1177, GOL1177, GOL1177, ILE155
Hydrophobic: ALA125, ALA125		NAG1176:H3 (2.092)5
144Conventional H-Bond: TYR159, GOL1177, ASN101, NAG1176
Electrostatic: GOL1177, ASP129
Carbon H-Bond: GOL1177, GOL1177
Hydrophobic: ALA125, ALA125, ILE155		TYR159:HH (2.223)7
Table 9. Lipophilicity, pharmacokinetics, druglikeness and medicinal chemistry of P. incisa L. identified compounds (1–17) based on their ADMET (for absorption, distribution, metabolism, excretion, and toxicity) properties.
Table 9. Lipophilicity, pharmacokinetics, druglikeness and medicinal chemistry of P. incisa L. identified compounds (1–17) based on their ADMET (for absorption, distribution, metabolism, excretion, and toxicity) properties.
Entry1234567891011121314151617
Lipophilicity and Physicochemical Properties
TPSA139.4863.32354.3146.53144.52153.7537.30210.51211.28211.28210.36190.3675.99100.1389.1398.3698.36
Log Po/w (iLOGP)0.711.080.872.430.831.641.960.941.501.922.232.612.412.442.582.982.84
Consensus Log Po/w−1.62−0.01−0.392.24−0.08−0.001.57−0.480.830.921.632.202.252.182.472.522.43
Log S (ESOL) solubility0.12−0.08−1.62−2.13−1.75−1.84−1.51−3.04−3.35−3.36−3.36−4.25−3.70−4.06−4.20−4.35−4.16
Pharmacokinetics
GI absorptionLowHighLowHighLowLowHighLowLowLowHighHighHigh YesHighHighHighHigh
BBB permeantNoNoNoYesNoNoYesNoNoNoNoNoYes NoNoNoNo
P-gp substrateNoNoNoNoNoNoNoNoYesYesNoNoNo NoNoNoNo
CYP1A2NoNoNoNoNoNoNoNoNoNoYesYesYes Yes YesYesYes
CYP2C19NoNoNoNoNoNoNoNoNoNoNoNoYes No NoNoNo
CYP2C9NoNoNoNoNoNoNoNoNoNoNoYesNoYes YesYesYes
CYP2D6NoNoNoNoNoNoNoNoNoNoYesYesNoYes YesYesYes
CYP3A4NoNoNoNoNoNoNoNoNoNoYesYesYesYes YesYesYes
Log Kp (skin permeation)−10.24−8.39−8.76−6.01−8.41−8.62−6.55−8.88−8.37−8.37−6.90−5.99−6.02−5.93−5.86−5.97−6.17
Druglikeness and Medicinal Chemistry
LipinskiYes YesYesYesYesYesYesNoNoNoYesYesYesYesYesYesYes
GhoseNoYesNoYesNoNoYes NoNoNoYes Yes Yes Yes Yes Yes Yes
VeberYesYesNoYesNoNoYes NoNoNoYes Yes Yes Yes Yes Yes Yes
ErganNoYesNoYesNoNoYes NoNoNoYes Yes Yes Yes Yes Yes Yes
MueggeNoNoNoNoYesNoNoNoNoNoYesYesYesYesYesYesYes
Leadlikeness0.550.550.110.550.560.550.550.170.110.110.550.550.550.550.550.550.55
Synthetic accessibility3.961.464.163.434.074.273.195.324.844.813.303.403.113.063.223.313.52
TPSA: Topological polar surface area; GI: gastrointestinal; BBB: blood–brain barrier; P-gp: P-glycoprotein; CYP: cytochrome P450.
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MDPI and ACS Style

Alreshidi, M.; Abdulhakeem, M.A.; Badraoui, R.; Amato, G.; Caputo, L.; De Martino, L.; Nazzaro, F.; Fratianni, F.; Formisano, C.; De Feo, V.; et al. Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis. Molecules 2023, 28, 7439. https://doi.org/10.3390/molecules28217439

AMA Style

Alreshidi M, Abdulhakeem MA, Badraoui R, Amato G, Caputo L, De Martino L, Nazzaro F, Fratianni F, Formisano C, De Feo V, et al. Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis. Molecules. 2023; 28(21):7439. https://doi.org/10.3390/molecules28217439

Chicago/Turabian Style

Alreshidi, Mousa, Mohammad A. Abdulhakeem, Riadh Badraoui, Giuseppe Amato, Lucia Caputo, Laura De Martino, Filomena Nazzaro, Florinda Fratianni, Carmen Formisano, Vincenzo De Feo, and et al. 2023. "Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis" Molecules 28, no. 21: 7439. https://doi.org/10.3390/molecules28217439

APA Style

Alreshidi, M., Abdulhakeem, M. A., Badraoui, R., Amato, G., Caputo, L., De Martino, L., Nazzaro, F., Fratianni, F., Formisano, C., De Feo, V., & Snoussi, M. (2023). Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis. Molecules, 28(21), 7439. https://doi.org/10.3390/molecules28217439

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