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Volume 20
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10.3390/ijms20010219
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Article
Peer-Review Record

Acute Myeloid Leukemia Affects Mouse Sperm Parameters, Spontaneous Acrosome Reaction, and Fertility Capacity

Int. J. Mol. Sci. 2019, 20(1), 219; https://doi.org/10.3390/ijms20010219
by Yulia Michailov 1,2, Eitan Lunenfeld 3, Joseph Kapilushnik 4,5, Shevach Friedler 2,5,6, Eckart Meese 7 and Mahmoud Huleihel 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2019, 20(1), 219; https://doi.org/10.3390/ijms20010219
Submission received: 6 December 2018 / Revised: 29 December 2018 / Accepted: 1 January 2019 / Published: 8 January 2019
(This article belongs to the Special Issue Molecular Biology of Spermatozoa)

Round  1

Reviewer 1 Report

¿Why the sperm viability was not assessed? Assessment of plasma membrane integrity could have increased the impact of this study.

 ¿What type of lectin‐binding it was used to assess the acrosome reaction, fluorescein conjugated peanut agglutinin (FITC-PNA) or Pisum sativum agglutinin (PSA)? 

 ¿Why were used 105 cells/100 of Murine C1498 (TIB-49) AML cells and 100 µl (3 mg/kg) of  Cytarabine in mice?

Author Response

Our answers to the comments and suggestions of the reviewer (in red color):

1. Why the sperm viability was not assessed? Assessment of plasma membrane integrity could have increased the impact of this study.

We thank the reviewer for this valuable comment. We will consider this comment in our future experiments.

 2. What type of lectin‐binding it was used to assess the acrosome reaction, fluorescein conjugated peanut agglutinin (FITC-PNA) or Pisum sativum agglutinin (PSA)? 

The type of lectin-binding was fluorescein conjugated peanut agglutinin (FITC-PNA). It was added in the text of the “MATERIALS AND METHODS” section, page # 9.

 3. Why were used 105 cells/100 of Murine C1498 (TIB-49) AML cells and 100 µl (3 mg/kg) of  Cytarabine in mice?

The experiment was performed according to Jian Min Lin et al., 2008. With some adaptation to our system. This statement was added in the “MATERIALS AND METHODS” section, page # 9. The reference was added into the “REFERENCE” section page 16 (reference # 42).

Reviewer 2 Report

The authors describe the detrimental effect of induced leukemia and chemotherapy on mice sperm concentration, motility and morphology as well as overall fertility. Although the results are not very surprising, with this work the authors have established and characterized a valuable animal model for further investigation of molecular mechanisms underlying fertility decrease in leukemic disease. Methods are correctly chosen and well described. The article is written clearly and concisely and may be accepted in the current form

Author Response

The reviewer had no comments.

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