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Article
Peer-Review Record

Cyclic Nigerosyl-Nigerose as Oxygen Nanocarrier to Protect Cellular Models from Hypoxia/Reoxygenation Injury: Implications from an In Vitro Model

Int. J. Mol. Sci. 2021, 22(8), 4208; https://doi.org/10.3390/ijms22084208
by Claudia Penna 1, Saveria Femminò 1,2, Fabrizio Caldera 3, Alberto Rubin Pedrazzo 3, Claudio Cecone 3, Edoardo Alfì 1, Stefano Comità 1, Takanobu Higashiyama 4, Francesco Trotta 3,*, Pasquale Pagliaro 1,* and Roberta Cavalli 5,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Reviewer 3:
Int. J. Mol. Sci. 2021, 22(8), 4208; https://doi.org/10.3390/ijms22084208
Submission received: 10 March 2021 / Revised: 30 March 2021 / Accepted: 14 April 2021 / Published: 19 April 2021
(This article belongs to the Special Issue Nano-Materials and Methods 3.0)

Round 1

Reviewer 1 Report

Penna et al., have addressed the concerns highlighted in their previous version of the manuscript. 

 Major concern:

Figure 8: The information presented in the Western blots are difficult to interpret. Please add a densitometric analysis alongside. 

Minor concerns: 

Methods. Section 4.8. Western blot analysis                                                 

i) Line 348: Figure 9, in place of 99.                                                                 

ii) Please mention the sources of the antibodies individually, including their dilution;  the relevant secondary antibodies, and their source(s) as well.

 

 

Author Response

We thanks the referee for the vakuable suggestions.  We have modified as requested the Western Blot and corrected the minor concerns.

Reviewer 2 Report

Thanks for addressing all the comments

Author Response

The authors thank the referee for well considering the revision.

Reviewer 3 Report

All mistakes were corrected by authors

Author Response

The authors thank the reviewer for the positive response.

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