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Article

The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response

by
Linnet Bischof
†,
Franziska Schweitzer
†,
Carolin C. Sterk
and
Jürgen J. Heinisch
*
Department of Genetics, Faculty of Biology/Chemistry, University of Osnabrueck, Barbarastrasse 11, D-49076 Osnabrueck, Germany
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(14), 7896; https://doi.org/10.3390/ijms23147896
Submission received: 20 June 2022 / Revised: 14 July 2022 / Accepted: 16 July 2022 / Published: 18 July 2022
(This article belongs to the Special Issue Small GTPases 2022)
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Abstract

:
Rho5, the yeast homolog of human Rac1, is a small GTPase which regulates the cell response to nutrient and oxidative stress by inducing mitophagy and apoptosis. It is activated by a dimeric GEF composed of the subunits Dck1 and Lmo1. Upon stress, all three proteins rapidly translocate from the cell surface (Rho5) and a diffuse cytosolic distribution (Dck1 and Lmo1) to mitochondria, with translocation of the GTPase depending on both GEF subunits. We here show that the latter associate with mitochondria independent from each other and from Rho5. The trapping of Dck1-GFP or GFP-Lmo1 to the mitochondrial surface by a specific nanobody fused to the transmembrane domain (TMD) of Fis1 results in a loss of function, mimicking the phenotypes of the respective gene deletions, dck1 or lmo1. Direct fusion of Rho5 to Fis1TMD, i.e., permanent attachment to the mitochondria, also mimics the phenotypes of an rho5 deletion. Together, these data suggest that the GTPase needs to be activated at the plasma membrane prior to its translocation in order to fulfill its function in the oxidative stress response. This notion is substantiated by the observation that strains carrying fusions of Rho5 to the cell wall integrity sensor Mid2, confining the GTPase to the plasma membrane, retained their function. We propose a model in which Rho5 activated at the plasma membrane represses the oxidative stress response under standard growth conditions. This repression is relieved upon its GEF-mediated translocation to mitochondria, thus triggering mitophagy and apoptosis.

1. Introduction

Yeast cells have evolved to respond to drastic changes in their environment by appropriately changing their metabolism and/or gene expression. For this purpose, different signalling cascades have been installed which frequently involve the use of small GTPases as molecular switches. A subfamily of the latter is comprised by the Rho-type GTPases (for “Ras homology”), six of which have been described in S. cerevisiae, namely Rho1 to Rho5, in addition to Cdc42 [1,2]. They mediate diverse physiological adaptations, ranging from cell wall integrity (CWI) signalling (Rho1 [3]) to cell polarity establishment in budding and cell fusion during mating (Cdc42 [4]). The switch is turned on by binding of a GTP molecule, and off by its hydrolysis to GDP [5,6,7]. The interconversion between these states is aided by the action of guanine nucleotide exchange factors (GEFs), which facilitate the exit of GDP and its substitution by GTP due to the higher intracellular concentration of the latter, and GTPase activating proteins (GAPs), which stimulate the intrinsic hydrolytic activity, respectively [8,9]. After their lipid modification at a C-terminally conserved cysteine residue (CAAX-box), the GTPases associate with cellular membranes, where they exert their physiological functions [10]. Cytosolic trafficking in the inactive state can be aided by masking the lipid anchor through association with a GDP-dissociation inhibitor protein (GDI, Ref. [11]).
Rho5 was the last member of the subfamily described in S. cerevisiae, then as a negative regulator of CWI signalling [12]. Later studies implicated its function in a variety of other signalling processes, suggesting that it may be a central hub in their coordination (reviewed in [13]). Briefly, the synthetic lethality of a hyperactive RHO5 allele with a ste50 deletion provided evidence that the GTPase works as a negative regulator in the high osmolarity glycerol (HOG) pathway, which could be viewed as having an opposite function to CWI signalling, as it responds to high rather than low medium osmolarity [14]. Moreover, the observed synthetic lethalities of rho5 ras2 and rho5 sch9 deletions suggest a repressing function of the GTPase in nutrient starvation [15]. In addition to its proposed role as a repressor of these signalling pathways, Rho5 was also found to regulate the response to oxidative stress, as judged from the hyper-resistance of its deletion mutants to hydrogen peroxide [16]. In fact, the GTPase was found to rapidly translocate from the plasma membrane to mitochondria upon exposure of the cells to this agent, a process dependent on the presence of its dimeric GEF (guanine nucleotide exchange factor), which is composed of two subunits encoded by DCK1 and LMO1 (Figure 1; ref. [17]).
In that work, a lack of either Rho5 or one of the GEF subunits was shown to drastically reduce mitophagy and apoptosis in S. cerevisiae, indicating that active Rho5 triggers these processes upon oxidative stress [17]. As the downstream MAP kinases in both the CWI and the HOG signalling pathway were shown to be required for mitophagy [18,19], it seems plausible that Rho5 at least partially acts by modulating their activity. A more direct role in mitophagy was also suggested from high-throughput screens, which identified Atg21 and Msp1, components of the mitophagy pathway and the outer mitochondrial membrane, respectively, as interaction partners of Rho5 [20].
Studies on the domain structure of Rho5 showed an unusual extension of 98 amino acids in the C-terminal half, which precedes the polybasic region (PBR) and the CAAX-box common in Rho-type GTPases [21]. All three regions were required for proper stress-induced translocation of yeast Rho5 [22]. Moreover, the trapping of GFP-tagged Rho5 to the mitochondrial surface in vivo by a specific nanobody rendered it non-functional with regard to oxidative stress, with phenotypes similar to the rho5 deletion. It was thus proposed that the GTPase needs to be activated at the plasma membrane in order to fulfill its role in mitophagy and apoptosis [22].
Translocation of homologs of Rho5 and the two GEF subunits to mitochondria was also observed under oxidative and nutrient stress in the more respiratory yeast K. lactis, in which Klrho5 deletions displayed pronounced morphological defects, in contrast to S. cerevisiae [23]. This is reminiscent of its human homolog Rac1, which, amongst a variety of cellular processes, regulates the dynamics of the actin cytoskeleton [24]. Like Rho5, Rac1 can be activated by the GEF DOCK180 in a complex with the ELMO protein, which are homologs of yeast Dck1 and Lmo1, respectively [25,26]. Malfunctions of Rac1 have been associated with serious diseases, including cancer and diabetes [27,28,29,30]. Consequently, yeast expression systems may provide excellent tools to study the molecular functions of the associated RAC1 alleles [23].
How mitochondrial translocation of yeast Rho5 is achieved, and, more importantly, how the physiological functions of Rho5 are related to its subcellular distribution, remains to be elucidated. In this work, we investigated whether the GEF subunits depend on each other and on Rho5 to translocate under oxidative stress. To gain some insight into the in vivo importance of this trafficking, the three proteins were confined to different subcellular microdomains, i.e., the plasma membrane and the mitochondrial surface. Phenotypic analyses suggest that Rho5 exerts important functions in oxidative stress response when still associated with the plasma membrane, rather than exclusively after its translocation to mitochondria.

2. Results

2.1. The GEF Subunits Dck1 and Lmo1 Translocate to Mitochondria Independent from Each Other or Rho5

While previous work revealed that oxidative stress-induced translocation of GFP-Rho5 to mitochondria depends on both subunits of the dimeric GEF Dck1/Lmo1 [17], whether the GEF subunits depend on the GTPase or on each other was not addressed. We thus checked the intracellular distribution of Dck1-GFP and Lmo1-GFP fusion proteins before and after the addition of hydrogen peroxide in different mutant backgrounds. Dck1- and Lmo1-GFP both displayed wild-type distributions when tested in a rho5 deletion background under standard growth conditions and upon oxidative stress (Figure 2a).
Interestingly, they were recruited to mitochondria independent from each other, as deletion of the gene encoding the other subunit (LMO1 or DCK1, respectively) had no effect on stress-induced translocation (Figure 2b). Thus, stress signals presumably act on both Dck1 and Lmo1 to provoke their rapid translocation to mitochondria, for which neither the formation of the dimeric GEF, nor that of a trimeric complex with Rho5 is required.

2.2. Trapping of Dck1 or Lmo1 to Mitochondria Impedes Rho5 Function in the Oxidative Stress Response

Given their independent translocation, we wondered whether the GEF subunits activate Rho5 at the mitochondrial surface to trigger the cell’s response to oxidative stress. To investigate this hypothesis, Dck1 and Lmo1 were trapped to the mitochondria, as previously exercised with GFP-Rho5, by using a “GFP binder” [22]. Therefore, the genes encoding the GEF subunits were tagged with GFP at their native loci, and combined by crossing and tetrad analyses with a strain carrying a specific GFP nanobody fused to the transmembrane domain of the mitochondrial outer membrane Fis1 protein. Since Lmo1-GFP constructs employed so far proved to be non-functional in subsequent physiological tests, i.e., they displayed a similar hyper-resistance towards hydrogen peroxide as the complete lmo1 deletion (data not shown), the C-terminal tag was substituted by GFP attached to the N-terminal end. Neither Dck1-GFP nor GFP-Lmo1 alone or in combination with the GFP-binder affected growth of the respective strains under standard conditions (Figure 3a).
Importantly, strains carrying only Dck1-GFP or GFP-Lmo1 were as sensitive to hydrogen peroxide as the wild-type, showing that both were fully functional. However, if efficiently trapped to the mitochondrial surface by the GFP-binder, as demonstrated by fluorescence microscopy (Figure 3b), a marked hyper-resistance of the respective strains was observed, mimicking the complete deletion in the case of GFP-Lmo1, and approaching the growth of the deletion strain for Dck1-GFP (Figure 3a). Consistent with the previous data on the trapping of the GFP-tagged GTPase itself, this suggested that in order to fulfill its physiological role in oxidative stress response, Rho5 has to be activated prior to reaching the mitochondria.

2.3. Attachment of Rho5 or its GEF Subunits to the Plasma Membrane Does Not Impair Its Repressor Function in Oxidative Stress Response

In light of the results described above, we attempted to trap the GTPase in a similar approach by appropriate constructs with the GFP nanobody attached to different plasma membrane proteins. However, this did not yield satisfactory results, as fluorescence microscopy showed that a substantial amount of GFP-Rho5 still translocated to mitochondria upon application of oxidative stress (unpublished results from our laboratory). Therefore, the RHO5 coding sequence was directly fused to the 3′end of the gene encoding the cell wall integrity sensor Mid2 at its native locus. The sensor was previously shown to reside in the plasma membrane in specific microdomains with a fairly uniform distribution, its C-terminus is exposed to the cytosol, and it is not subject to rapid endocytosis [31,32]. Strains carrying the MID2-RHO5 fusion in conjunction with a rho5 deletion grew like wild-type under normal conditions and in the presence of hydrogen peroxide (Figure 4).
This suggested that Rho5 at the plasma membrane suffices to trigger the appropriate response to oxidative stress under these conditions, i.e., that translocation of Rho5 and its GEF to mitochondria, is not required. In contrast to previous findings on the pronounced the hyper-sensitivity of strains with an activated RHO5G12V allele towards oxidative stress [22], a pronounced difference as compared to the wild-type was found when the allele was fused with the MID2 coding sequence. Vice versa, a direct fusion of Rho5 with the transmembrane domain of Fis1, which confines the GTPase to the outer mitochondrial membrane, rendered the strains as hyper-resistant towards hydrogen peroxide as a rho5 deletion (Figure 4). This indicates a lack of GTPase function in the fusion protein, in accordance with previous data from trapping of Rho5 to mitochondria via a GFP nanobody [22].

3. Discussion

Rho5 in S. cerevisiae has been implicated, amongst other functions, in linking the oxidative stress response to mitochondrial turnover and apoptosis [16]. This notion is consistent with the rapid translocation of Rho5 and that of its dimeric GEF Dck1/Lmo1 to mitochondria upon exposure to hydrogen peroxide [17]. We here provided evidence that the translocation occurs for each individual GEF subunit, independent from each other and Rho5. Previous models proposed that the trimeric complex between the GTPase and its GEF could move as an entity from the plasma membrane to the mitochondrial surface [17,22], analogous to the complex suggested to be formed by the human homolog Rac1 with the DOCK180/ELMO [25]. The independent translocation of the GEF subunits observed here points to an alternative mechanism in which the GEF dimer could first be assembled at the mitochondrial surface and only then recruit Rho5. Such a sequence has been suggested for the Rab-GTPase Ypt7 involved in vesicle transport, which is recruited to and activated by its GEF associated with the target membrane [33]. In this context, human Rac1 also associates with various subcellular compartments, frequently mediated by its interaction with a plethora of specific GEFs (reviewed in [34]). It would therefore be interesting to determine the exact timing of the appearance of Dck1, Lmo1, and Rho5 at yeast mitochondria upon exposure to oxidative stress, or if they indeed travel together as a trimeric complex. Given that the translocation occurs within a matter of seconds [17], this may be challenging.
More importantly, we here addressed the physiological function of the intracellular distribution of the dimeric GEF by trapping either subunit to the mitochondrial surface through a GFP-tag and the corresponding nanobody. This rendered the strains hyper-resistant to oxidative stress, mimicking the phenotype of dck1 and lmo1 deletions. Similarly, confining Rho5 to the outer mitochondrial membrane by fusion with the transmembrane domain of Fis1 left the GTPase non-functional, showing the same hyper-resistance as a complete rho5 deletion. These findings are consistent with previous data obtained from the nanobody-mediated trapping of GFP-Rho5 to mitochondria and support the notion that the GTPase needs to be activated at the plasma membrane prior to its translocation [22]. In contrast, the fusion of Rho5 to the plasma membrane sensor Mid2 did not affect the cell response to oxidative stress under the conditions tested herein. It should be noted that Mid2 is known to accumulate in membrane microdomains, which would cause an increased local concentration of the fused GTPase [31], in analogy to its human homolog, where local clustering of Rac1 was proposed as a mechanism of activation [35].
How is the localization and activity of Rho5 related to the oxidative stress response? The activated plasma membrane-bound Rho5 is believed to repress various signalling pathways, including CWI [12] and HOG [16]. In this context, upon exposure to hydrogen peroxide the CWI pathway was shown to transmit a signal generated by its sensors to the downstream MAP kinase Slt2 [36]. The kinase phosphorylates Cnc1, a cyclin regulating the cyclin-dependent kinase Cdk8, which triggers the nuclear exit of Cnc1. The latter apparently serves two functions: (i) it represses stress responsive genes in the nucleus in association with the mediator complex; and (ii) upon its expulsion into the cytosol, it associates with mitochondria and leads to their fission, mitophagy, and cell death [37]. However, it should be noted that the latter effect was observed under conditions of nitrogen starvation rather than with oxidative stress. Nevertheless, a lack of active Rho5 at the plasma membrane would be expected to increase CWI signalling, resulting in phosphorylation and nuclear exit of Cnc1, which could trigger mitophagy and apoptosis. How this could be related to a recent report on the involvement of the CWI sensor Mtl1 in the induction of autophagy and mitophagy upon glucose starvation during diauxic shift [38] also remains to be investigated.
In addition to these rather indirect actions of Rho5, we believe that its direct association with mitochondria also triggers oxidative stress-induced mitophagy and apoptosis. This requires activation of the GTPase prior to its translocation, as indicated by the null phenotypes of trapping either of the GEF subunits or Rho5 itself to the mitochondrial surface. On the other hand, a similar trapping of the activated GFP-Rho5G12V variant restored sensitivity towards hydrogen peroxide to wild-type levels, whereas the untagged hyperactive GTPase was hyper-sensitive [22]. The fact that confining the hyper-active variant to the plasma membrane with the Mid2-Rho5G12V fusion did not markedly increase sensitivity to oxidative stress as compared to the wild-type suggests that the intracellular trafficking of Rho5 also plays an important role.

4. Materials and Methods

4.1. Strains and Growth Conditions

Yeast strains employed and their genotypes are listed in Table 1. All strains derived from the HD56-5A and its isogenic diploid DHD5, which are closely related to the CEN.PK background [39,40].
The yeast cell culture and genetic techniques followed standard procedures [41]. Rich medium (YEPD) contained 1% yeast extract, 2% Bacto peptone (Difco Laboratories Inc., Detroit, MI, USA), and 2% glucose. Synthetic media were prepared as described in [41], with the omission of amino acids or bases as required for selection of plasmids or deletion markers and 2% glucose (SCD). For selection of the kanMX marker, 100 mg/L of G418 were added to the medium after sterilization.
Growth curves were obtained in 100 µL cultures in 96 well plates, in SCD with or without hydrogen peroxide as indicated, and recorded in a Varioscan Lux plate reader (Thermo Scientific, Bremen, Germany) as described previously [22].
For tetrad analyses, diploid strains were grown to stationary phase in liquid YEPD, collected by centrifugation and dropped onto 1% potassium acetate plates for sporulation at 30 °C. After two to three days and microscopic inspection for ascus formation, a sample of each culture was resuspended in 100 µL of sterile water and 4 µL of Zymolyase 100T (10 mg/mL) was added, followed by 7–10 min incubation at room temperature. 15 µL of the suspension was streaked out onto a YEPD plate and spores were segregated using a Singer MSM400 micromanipulator (Singer Instruments, Somerset, UK). Plates were incubated for 4 days at 30 °C, and colony formation was documented by scanning. Scanned images were adjusted for brightness and contrast using Corel Photo Paint with the same settings for the entire plate prior to compilation of sections into the final figures.
For manipulations in E. coli, strain DH5α was employed with standard media as described previously [32].

4.2. Construction of Plasmids, Deletion Mutants and Gene Tagging

Wild-type genes from S. cerevisiae were obtained by PCR using appropriate oligonucleotides with restriction sites and genomic DNA of strain DHD5 or its derivatives as templates. Deletion strains and gene fusions with specific tags were obtained by one-step gene replacement techniques [42] by adding 45-50 bp of flanking sequences homologous to the target gene with appropriate oligonucleotides used for PCR amplification. For selection of in vivo recombinants, either the kanMX or the SkHIS3 cassette from the Longtine collection [43], SpHIS5 from pUG27, or KlLEU2 from pUG73 were employed [44]. GFP tags were amplified from pJJH1619 (GFP-kanMX) or pJJH1620 (GFP-SkHIS3); mCherry tags from pJJH1524 (mCherry-SkHIS3), described in [23]. As a mitochondrial marker for fluorescence microscopy, either a genomic IDP1-mCherry fusion [22] was introduced by crossing with the appropriate strains and tetrad analysis, or plasmid pJJH1408 [17] was employed, which encodes a fusion of the Cox4-mitochondrial signal sequence with mCherry.
For integration at the leu2-3,112 locus, constructs were subcloned into the vector YIplac128 [45], and the resulting plasmids were linearized by digestion with BstEII prior to transformation and selection for leucine prototrophy. Proper integration was confirmed by PCR. Specifically, pLAO12 (PFK2p-GB-FIS1TMD) was used to integrate the GFP-nanobody construct, and pJJH3024 (RHO5-FIS1TMD) and pJJH3096 (RHO5G12V-FIS1TMD) for integration of the respective RHO5 alleles fused to the mitochondrial transmembrane domain coding sequence.
All PCR-generated fragments were verified by Sanger sequencing (Seqlab, Göttingen, Germany). Maps and sequences of all plasmids and modifications of genomic loci are available upon request.

4.3. Fluorescence Microscopy

The setup used for the fluorescence microscopy consisted of a Zeiss Axioplan 2 (Carl Zeiss, Jena, Germany) equipped with a 100× alpha-Plan Fluor objective (NA 1.45) and differential-interference contrast. Sample handling and image processing have been described in detail in [17].

5. Conclusions

In conclusion, we assume that the GTPase has a dual role with regard to the oxidative stress response: while at the plasma membrane it represses the expression of stress-responsive nuclear genes through the CWI/Cnc1 relay under standard growth conditions. When stressed by hydrogen peroxide, Rho5 dissociates from the plasma membrane and thus relieves repression, priming the cells for mitophagy and apoptosis. The association of Rho5 with its GEF at the mitochondrial surface would then further promote the path to death. Further experiments to test the fusions obtained herein for their effect on mitophagy and apoptosis under strong oxidative stress are therefore required and currently in progress.

Author Contributions

Conceptualization, J.J.H.; methodology, L.B., F.S. and C.C.S.; software, L.B., F.S. and C.C.S.; validation, L.B., F.S. and C.C.S.; formal analysis, L.B., F.S., C.C.S. and J.J.H.; investigation, L.B., F.S. and C.C.S.; resources, J.J.H.; data curation, L.B., F.S. and C.C.S.; writing—original draft preparation, J.J.H.; writing—review and editing, L.B., F.S., C.C.S. and J.J.H.; visualization, L.B., F.S. and C.C.S.; supervision, J.J.H.; project administration, J.J.H.; funding acquisition, J.J.H. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by a grant from the Deutsche Forschungsgemeinschaft (DFG; HE 1880/6-1).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We are grateful to Hans-Peter Schmitz for invaluable advice in fluorescence microscopy, statistical analyses, and reading of the manuscript. We also thank Andrea Murra for excellent technical assistance and Rosaura Rodicio for critical reading of the manuscript. We also acknowledge support by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publishing Fund of Osnabrück University.

Conflicts of Interest

The authors declare that they have no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Abbreviations

CEN/ARS, centromere and autonomously replicating sequence of yeast plasmids with low copy numbers; CWI, Cell Wall Integrity pathway; DIC, Differential Interference Phase Contrast; GAP, GTPase Activating Protein; GDI, GDP Dissociation Inhibitor; GEF, Guanine nucleotide Exchange Factor; GFP-nb, GFP nanobody (also GB for GFP binder); HOG, High Osmolarity Glycerol pathway; PBR, Poly-Basic Region; RHO5G12V, allele of the yeast RHO5 gene encoding an activated variant of the GTPase; SCD, synthetic complete medium with glucose; YEPD, rich medium with glucose; TMD, Trans-Membrane Domain.

References

  1. Hall, A. Rho family GTPases. Biochem. Soc. Trans. 2012, 40, 1378–1382. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Perez, P.; Rincon, S.A. Rho GTPases: Regulation of cell polarity and growth in yeasts. Biochem. J. 2010, 426, 243–253. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Sekiya-Kawasaki, M.; Abe, M.; Saka, A.; Watanabe, D.; Kono, K.; Minemura-Asakawa, M.; Ishihara, S.; Watanabe, T.; Ohya, Y. Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae. Genetics 2002, 162, 663–676. [Google Scholar] [CrossRef]
  4. Smith, J.A.; Hall, A.E.; Rose, M.D. Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion. J. Cell Biol. 2017, 216, 3971–3980. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Bishop, A.L.; Hall, A. Rho GTPases and their effector proteins. Biochem. J. 2000, 348 Pt 2, 241–255. [Google Scholar] [CrossRef]
  6. Bos, J.L.; Rehmann, H.; Wittinghofer, A. GEFs and GAPs: Critical elements in the control of small G proteins. Cell 2007, 129, 865–877. [Google Scholar] [CrossRef] [Green Version]
  7. Takai, Y.; Sasaki, T.; Matozaki, T. Small GTP-binding proteins. Physiol. Rev. 2001, 81, 153–208. [Google Scholar] [CrossRef]
  8. Hodge, R.G.; Ridley, A.J. Regulating Rho GTPases and their regulators. Nat. Rev. Mol. Cell. Biol. 2016, 17, 496–510. [Google Scholar] [CrossRef]
  9. Wennerberg, K.; Der, C.J. Rho-family GTPases: It’s not only Rac and Rho (and I like it). J. Cell Sci. 2004, 117, 1301–1312. [Google Scholar] [CrossRef] [Green Version]
  10. Roberts, P.J.; Mitin, N.; Keller, P.J.; Chenette, E.J.; Madigan, J.P.; Currin, R.O.; Cox, A.D.; Wilson, O.; Kirschmeier, P.; Der, C.J. Rho Family GTPase modification and dependence on CAAX motif-signaled posttranslational modification. J. Biol. Chem. 2008, 283, 25150–25163. [Google Scholar] [CrossRef] [Green Version]
  11. Tiedje, C.; Sakwa, I.; Just, U.; Hofken, T. The Rho GDI Rdi1 regulates Rho GTPases by distinct mechanisms. Mol. Biol. Cell. 2008, 19, 2885–2896. [Google Scholar] [CrossRef] [Green Version]
  12. Schmitz, H.P.; Huppert, S.; Lorberg, A.; Heinisch, J.J. Rho5p downregulates the yeast cell integrity pathway. J. Cell Sci. 2002, 115, 3139–3148. [Google Scholar] [CrossRef] [PubMed]
  13. Hühn, J.; Musielak, M.; Schmitz, H.P.; Heinisch, J.J. Fungal homologues of human Rac1 as emerging players in signal transduction and morphogenesis. Int. Microbiol. 2020, 23, 43–53. [Google Scholar] [CrossRef] [PubMed]
  14. Annan, R.B.; Wu, C.; Waller, D.D.; Whiteway, M.; Thomas, D.Y. Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination. Eukaryot. Cell. 2008, 7, 1441–1449. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Schmitz, H.P.; Jendretzki, A.; Sterk, C.; Heinisch, J.J. The small yeast GTPase Rho5 and its dimeric GEF Dck1/Lmo1 respond to glucose starvation. Int. J. Mol. Sci. 2018, 19, 2186. [Google Scholar] [CrossRef] [Green Version]
  16. Singh, K.; Kang, P.J.; Park, H.O. The Rho5 GTPase is necessary for oxidant-induced cell death in budding yeast. Proc. Natl. Acad. Sci. USA 2008, 105, 1522–1527. [Google Scholar] [CrossRef] [Green Version]
  17. Schmitz, H.P.; Jendretzki, A.; Wittland, J.; Wiechert, J.; Heinisch, J.J. Identification of Dck1 and Lmo1 as upstream regulators of the small GTPase Rho5 in Saccharomyces cerevisiae. Mol. Microbiol. 2015, 96, 306–324. [Google Scholar] [CrossRef] [Green Version]
  18. Mao, K.; Klionsky, D.J. MAPKs regulate mitophagy in Saccharomyces cerevisiae. Autophagy 2011, 7, 1564–1565. [Google Scholar] [CrossRef] [Green Version]
  19. Mao, K.; Wang, K.; Zhao, M.; Xu, T.; Klionsky, D.J. Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae. J. Cell Biol. 2011, 193, 755–767. [Google Scholar] [CrossRef] [Green Version]
  20. Singh, K.; Lee, M.E.; Entezari, M.; Jung, C.H.; Kim, Y.; Park, Y.; Fioretti, J.D.; Huh, W.K.; Park, H.O.; Kang, P.J. Genome-wide studies of Rho5-interacting proteins that are involved in oxidant-induced cell death in budding yeast. G3 Genes Genomes Genet. 2019, 9, 921–931. [Google Scholar] [CrossRef] [Green Version]
  21. Lam, B.D.; Hordijk, P.L. The Rac1 hypervariable region in targeting and signaling: A tail of many stories. Small GTPases 2013, 4, 78–89. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Sterk, C.; Graber, L.; Schmitz, H.P.; Heinisch, J.J. Analysis of functional domains in Rho5, the yeast homolog of human Rac1 GTPase, in oxidative stress response. Int. J. Mol. Sci. 2019, 20, 5550. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Musielak, M.; Sterk, C.C.; Schubert, F.; Meyer, C.; Paululat, A.; Heinisch, J.J. The small GTPase KlRho5 responds to oxidative stress and affects cytokinesis. J. Cell Sci. 2021, 134, jcs258301. [Google Scholar] [CrossRef] [PubMed]
  24. Grimsley, C.M.; Kinchen, J.M.; Tosello-Trampont, A.C.; Brugnera, E.; Haney, L.B.; Lu, M.; Chen, Q.; Klingele, D.; Hengartner, M.O.; Ravichandran, K.S. Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migration. J. Biol. Chem. 2004, 279, 6087–6097. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Brugnera, E.; Haney, L.; Grimsley, C.; Lu, M.; Walk, S.F.; Tosello-Trampont, A.C.; Macara, I.G.; Madhani, H.; Fink, G.R.; Ravichandran, K.S. Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat. Cell Biol. 2002, 4, 574–582. [Google Scholar] [CrossRef]
  26. Cote, J.F.; Vuori, K. Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J. Cell Sci. 2002, 115, 4901–4913. [Google Scholar] [CrossRef] [Green Version]
  27. Gadea, G.; Blangy, A. Dock-family exchange factors in cell migration and disease. Eur. J. Cell Biol. 2014. [Google Scholar] [CrossRef]
  28. Marei, H.; Malliri, A. Rac1 in human diseases: The therapeutic potential of targeting Rac1 signaling regulatory mechanisms. Small GTPases 2017, 8, 139–163. [Google Scholar] [CrossRef] [Green Version]
  29. Olson, M.F. Rho GTPases, their post-translational modifications, disease-associated mutations and pharmacological inhibitors. Small GTPases. 2018, 9, 203–215. [Google Scholar] [CrossRef]
  30. Takenaka, N.; Nihata, Y.; Satoh, T. Rac1 activation caused by membrane translocation of a guanine nucleotide exchange factor in Akt2-mediated insulin signaling in mouse skeletal muscle. PLoS ONE 2016, 11, e0155292. [Google Scholar] [CrossRef] [Green Version]
  31. Kock, C.; Arlt, H.; Ungermann, C.; Heinisch, J.J. Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling. Cell. Microbiol. 2016, 18, 1251–1267. [Google Scholar] [CrossRef] [Green Version]
  32. Straede, A.; Heinisch, J.J. Functional analyses of the extra- and intracellular domains of the yeast cell wall integrity sensors Mid2 and Wsc1. FEBS Lett. 2007, 581, 4495–4500. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Borchers, A.C.; Langemeyer, L.; Ungermann, C. Who’s in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond. J. Cell Biol. 2021, 220, e202105120. [Google Scholar] [CrossRef] [PubMed]
  34. Payapilly, A.; Malliri, A. Compartmentalisation of RAC1 signalling. Curr. Opin. Cell Biol. 2018, 54, 50–56. [Google Scholar] [CrossRef] [Green Version]
  35. Remorino, A.; De Beco, S.; Cayrac, F.; Di Federico, F.; Cornilleau, G.; Gautreau, A.; Parrini, M.C.; Masson, J.B.; Dahan, M.; Coppey, M. Gradients of Rac1 nanoclusters support spatial patterns of Rac1 signaling. Cell Rep. 2017, 21, 1922–1935. [Google Scholar] [CrossRef] [Green Version]
  36. Jin, C.; Strich, R.; Cooper, K.F. Slt2p phosphorylation induces cyclin C nuclear-to-cytoplasmic translocation in response to oxidative stress. Mol. Biol. Cell. 2014, 25, 1396–1407. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Willis, S.D.; Hanley, S.E.; Beishke, T.; Tati, P.D.; Cooper, K.F. Ubiquitin-proteasome-mediated cyclin C degradation promotes cell survival following nitrogen starvation. Mol. Biol. Cell. 2020, 31, 1015–1031. [Google Scholar] [CrossRef] [PubMed]
  38. Montella-Manuel, S.; Pujol-Carrion, N.; de la Torre-Ruiz, M.A. The cell wall integrity receptor Mtl1 contributes to articulate autophagic responses when glucose availability is compromised. J. Fungi 2021, 7, 903. [Google Scholar] [CrossRef]
  39. Kirchrath, L.; Lorberg, A.; Schmitz, H.P.; Gengenbacher, U.; Heinisch, J.J. Comparative genetic and physiological studies of the MAP kinase Mpk1p from Kluyveromyces lactis and Saccharomyces cerevisiae. J. Mol. Biol. 2000, 300, 743–758. [Google Scholar] [CrossRef]
  40. Schacherer, J.; Ruderfer, D.M.; Gresham, D.; Dolinski, K.; Botstein, D.; Kruglyak, L. Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains. PLoS ONE 2007, 2, e322. [Google Scholar] [CrossRef] [Green Version]
  41. Rose, M.D.; Winston, F.; Hieter, P. Methods in Yeast Genetics; Cold Spring Harbor Laboratory Press: New York, NY, USA, 1990. [Google Scholar]
  42. Rothstein, R. Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast. Methods Enzymol. 1991, 194, 281–301. [Google Scholar] [CrossRef] [PubMed]
  43. Longtine, M.S.; McKenzie, A., 3rd; Demarini, D.J.; Shah, N.G.; Wach, A.; Brachat, A.; Philippsen, P.; Pringle, J.R. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 1998, 14, 953–961. [Google Scholar] [CrossRef]
  44. Gueldener, U.; Heinisch, J.; Koehler, G.J.; Voss, D.; Hegemann, J.H. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res. 2002, 30, e23. [Google Scholar] [CrossRef] [Green Version]
  45. Gietz, R.D.; Sugino, A. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 1988, 74, 527–534. [Google Scholar] [CrossRef]
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Figure 1. Proposed role of the intracellular distribution of Rho5 and its dimeric GEF Dck1/Lmo1 in the oxidative stress response. Rho5 is inactive in its GDP-bound and active in its GTP-bound state, which are interconverted by the help of a GTPase activating protein (GAP, Rgd2) and the dimeric GDP/GTP exchange factor (GEF, Dck1/Lmo1). Negative regulation (lines with bars) of the cell wall integrity pathway (CWI) and the high osmolarity glycerol pathway (HOG) is indicated for the active Rho5 associated with the plasma membrane by its lipid anchor (wavy line). A possible indirect effect of the CWI pathway on mitophagy and apoptosis (see discussion section) is symbolized by the dashed arrow. Dotted arrows show proposed routes of intracellular trafficking of the GTPase and its GEF subunits under different physiological conditions. Fusion constructs to confine Rho5 to either the plasma membrane through the CWI sensor Mid2, or to the mitochondrial outer membrane through the transmembrane domain (TMD) of Fis1, either directly or fused to a GFP nanobody (GFP-nb), constructed in this work are also indicated. Phenotypes regarding oxidative stress response are highlighted in blue print.
Figure 1. Proposed role of the intracellular distribution of Rho5 and its dimeric GEF Dck1/Lmo1 in the oxidative stress response. Rho5 is inactive in its GDP-bound and active in its GTP-bound state, which are interconverted by the help of a GTPase activating protein (GAP, Rgd2) and the dimeric GDP/GTP exchange factor (GEF, Dck1/Lmo1). Negative regulation (lines with bars) of the cell wall integrity pathway (CWI) and the high osmolarity glycerol pathway (HOG) is indicated for the active Rho5 associated with the plasma membrane by its lipid anchor (wavy line). A possible indirect effect of the CWI pathway on mitophagy and apoptosis (see discussion section) is symbolized by the dashed arrow. Dotted arrows show proposed routes of intracellular trafficking of the GTPase and its GEF subunits under different physiological conditions. Fusion constructs to confine Rho5 to either the plasma membrane through the CWI sensor Mid2, or to the mitochondrial outer membrane through the transmembrane domain (TMD) of Fis1, either directly or fused to a GFP nanobody (GFP-nb), constructed in this work are also indicated. Phenotypes regarding oxidative stress response are highlighted in blue print.
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Figure 2. Requirements for translocation of Dck1 and Lmo1 to mitochondria under oxidative stress. (a) Dck1 and Lmo1 translocate independently from the presence of Rho5. (b) Dck1 and Lmo1 also translocate independent from each other. Strains expressing the GFP fusions of the indicated proteins from their native genomic loci were used to introduce a mitochondrial marker tagged with mCherry on a CEN/ARS plasmid (pJJH1408). Transformants were grown in selective minimal media. Representative images for each strain and condition are shown. When oxidative stress was applied by the addition of 4.4 mM hydrogen peroxide (+H2O2), fluorescence images were taken within less than 15 min with the indicated channels (GFP/mCherry), or using differential interference phase contrast (DIC). The percentage of cells displaying colocalization of the two fluorescence markers at mitochondria (mit) is given together with the number of total cells inspected (n). The size bars in the DIC images correspond to 5 µm, which is applicable to all images in the same panel. The strains employed were Dck1-GFP rho5Δ = HCSO20; Lmo1-GFP rho5Δ = HCSO25; Dck1-GFP lmo1Δ = HCSO26; and Lmo1-GFP dck1Δ = HCSO33. It should be noted that the C-terminal Lmo1-GFP fusion did not complement the phenotypic defects of a lmo1 deletion, indicating that the tagged protein is not functional in vivo. However, a functional N-terminal GFP-Lmo1 fusion employed for the physiological studies in subsequent experiments was checked in the dck1 and rho5 deletion strains and confirmed the independent translocation.
Figure 2. Requirements for translocation of Dck1 and Lmo1 to mitochondria under oxidative stress. (a) Dck1 and Lmo1 translocate independently from the presence of Rho5. (b) Dck1 and Lmo1 also translocate independent from each other. Strains expressing the GFP fusions of the indicated proteins from their native genomic loci were used to introduce a mitochondrial marker tagged with mCherry on a CEN/ARS plasmid (pJJH1408). Transformants were grown in selective minimal media. Representative images for each strain and condition are shown. When oxidative stress was applied by the addition of 4.4 mM hydrogen peroxide (+H2O2), fluorescence images were taken within less than 15 min with the indicated channels (GFP/mCherry), or using differential interference phase contrast (DIC). The percentage of cells displaying colocalization of the two fluorescence markers at mitochondria (mit) is given together with the number of total cells inspected (n). The size bars in the DIC images correspond to 5 µm, which is applicable to all images in the same panel. The strains employed were Dck1-GFP rho5Δ = HCSO20; Lmo1-GFP rho5Δ = HCSO25; Dck1-GFP lmo1Δ = HCSO26; and Lmo1-GFP dck1Δ = HCSO33. It should be noted that the C-terminal Lmo1-GFP fusion did not complement the phenotypic defects of a lmo1 deletion, indicating that the tagged protein is not functional in vivo. However, a functional N-terminal GFP-Lmo1 fusion employed for the physiological studies in subsequent experiments was checked in the dck1 and rho5 deletion strains and confirmed the independent translocation.
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Figure 3. Trapping of GFP-tagged Dck1 and Lmo1 to mitochondria results in hyper-resistance towards oxidative stress. (a) Growth of strains producing Dck1-GFP (upper panel) or GFP-Lmo1 (lower panel) fusion proteins encoded at their native genomic loci in combination with a GFP binding nanobody attached to the mitochondrial surface (GB-FIS1TMD) in the absence (left) or presence (right) of hydrogen peroxide. Error bars give the standard deviations from growth recorded in duplicate for two independent strains, each, with the exception of the wild-type strain, for whom only one was measured in duplicate. Strains employed in the upper panel were: wild-type = HLBO20-4B; dck1Δ = HD56-5A/dck1ΔH1 and HD56-5A/dck1ΔKL6; DCK1-GFP = HLBO22-2A and HLBO22-4D; GB-FIS1 = HLBO22-2D and HLBO22-9D; DCK1-GFP + GB-FIS1 = HLBO22-3B and HLBO22-5B; and in the lower panel: wild-type = HLBO20-4B; lmo1Δ = LBO81 and HOD464-7B; LMO1-GFP = HCLO01-3B and HLBO19-3A; GB-FIS1 = HCLO01-8B and HCLO01-27D; LMO1-GFP + GB-FIS1 = HCLO01-15C and HCLO01-23D. (b) Dck1-GFP and GFP-Lmo1 are efficiently recruited to the mitochondrial surface by a nanobody fused to the transmembrane domain of Fis1. Fluorescence microscopy images were taken for cells grown in synthetic medium with 2% glucose as explained in the legend of Figure 2. An Idp1-mCherry fusion encoded at the native IDP1 locus was used as a mitochondrial marker. Percentages of cells showing a colocalization of the tagged GEF subunits with mitochondria (mit) are given, calculated from the total number of cells inspected (n). Size bars correspond to 5 µm. The strains employed were DCK1-GFP = HLBO21-2A and HLBO21-10A; DCK1-GFP + GB-FIS1 = HLBO21-3D and HLBO21-6A; GFP-LMO1 = HLBO19-3B and HLBO19-5A; GFP-LMO1 + GB-FIS1 = HLBO19-1B and HLBO19-8C.
Figure 3. Trapping of GFP-tagged Dck1 and Lmo1 to mitochondria results in hyper-resistance towards oxidative stress. (a) Growth of strains producing Dck1-GFP (upper panel) or GFP-Lmo1 (lower panel) fusion proteins encoded at their native genomic loci in combination with a GFP binding nanobody attached to the mitochondrial surface (GB-FIS1TMD) in the absence (left) or presence (right) of hydrogen peroxide. Error bars give the standard deviations from growth recorded in duplicate for two independent strains, each, with the exception of the wild-type strain, for whom only one was measured in duplicate. Strains employed in the upper panel were: wild-type = HLBO20-4B; dck1Δ = HD56-5A/dck1ΔH1 and HD56-5A/dck1ΔKL6; DCK1-GFP = HLBO22-2A and HLBO22-4D; GB-FIS1 = HLBO22-2D and HLBO22-9D; DCK1-GFP + GB-FIS1 = HLBO22-3B and HLBO22-5B; and in the lower panel: wild-type = HLBO20-4B; lmo1Δ = LBO81 and HOD464-7B; LMO1-GFP = HCLO01-3B and HLBO19-3A; GB-FIS1 = HCLO01-8B and HCLO01-27D; LMO1-GFP + GB-FIS1 = HCLO01-15C and HCLO01-23D. (b) Dck1-GFP and GFP-Lmo1 are efficiently recruited to the mitochondrial surface by a nanobody fused to the transmembrane domain of Fis1. Fluorescence microscopy images were taken for cells grown in synthetic medium with 2% glucose as explained in the legend of Figure 2. An Idp1-mCherry fusion encoded at the native IDP1 locus was used as a mitochondrial marker. Percentages of cells showing a colocalization of the tagged GEF subunits with mitochondria (mit) are given, calculated from the total number of cells inspected (n). Size bars correspond to 5 µm. The strains employed were DCK1-GFP = HLBO21-2A and HLBO21-10A; DCK1-GFP + GB-FIS1 = HLBO21-3D and HLBO21-6A; GFP-LMO1 = HLBO19-3B and HLBO19-5A; GFP-LMO1 + GB-FIS1 = HLBO19-1B and HLBO19-8C.
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Ijms 23 07896 g004 550
Figure 4. A fusion of Rho5 to the plasma membrane sensor Mid2 still functions in the oxidative stress response. (a) Growth of strains carrying the indicated fusions with RHO5 either at the MID2 locus (MID2-RHO5, MID2-RHO5G12V) or integrated with plasmid pJJH3024 at the leu2-3,112 locus (RHO5-FIS1TMD) was followed under standard conditions (left) and under oxidative stress (right). Wild-type and rho5 deletion strains were included as controls. Error bars give the standard deviations from growth recorded in duplicate for two independent strains, each, with the exception of MID2-RHO5G12V, for which only one strain was constructed. Strains employed were: wild type = FSO4-3A and FSO4-8A, rho5 = FSO4-3D and FSO4-8B, MID2-RHO5 = HOD500-10A and HOD500-11B, MID2-RHO5G12V = HFSO83, RHO5-FIS1TMD = LBO130 and LBO132, RHO5G12V-FIS1TMD = HOD529-2B and HOD529-2B. (b) Mid2-GFP-Rho5 fusions are confined to the plasma membrane with and without oxidative stress. Strain HOD512-3A was subjected to life cell fluorescence microscopy and representative bright-field (DIC, left panels) and fluorescence images (GFP channel; right panels) are shown. Percentages of cells with localization of the fusion protein at the plasma membrane (PM) were calculated from the total number of cells (n) observed.
Figure 4. A fusion of Rho5 to the plasma membrane sensor Mid2 still functions in the oxidative stress response. (a) Growth of strains carrying the indicated fusions with RHO5 either at the MID2 locus (MID2-RHO5, MID2-RHO5G12V) or integrated with plasmid pJJH3024 at the leu2-3,112 locus (RHO5-FIS1TMD) was followed under standard conditions (left) and under oxidative stress (right). Wild-type and rho5 deletion strains were included as controls. Error bars give the standard deviations from growth recorded in duplicate for two independent strains, each, with the exception of MID2-RHO5G12V, for which only one strain was constructed. Strains employed were: wild type = FSO4-3A and FSO4-8A, rho5 = FSO4-3D and FSO4-8B, MID2-RHO5 = HOD500-10A and HOD500-11B, MID2-RHO5G12V = HFSO83, RHO5-FIS1TMD = LBO130 and LBO132, RHO5G12V-FIS1TMD = HOD529-2B and HOD529-2B. (b) Mid2-GFP-Rho5 fusions are confined to the plasma membrane with and without oxidative stress. Strain HOD512-3A was subjected to life cell fluorescence microscopy and representative bright-field (DIC, left panels) and fluorescence images (GFP channel; right panels) are shown. Percentages of cells with localization of the fusion protein at the plasma membrane (PM) were calculated from the total number of cells (n) observed.
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Table
Table 1. Strains constructed in this work.
Table 1. Strains constructed in this work.
Strain NameGenotype 1
FSO4-3AMATalpha ura3-52 his3-11,15 leu2-3,112
FSO4-3DMATalpha ura3-52 his3-11,15 leu2-3,112 rho5::kanMX
FSO4-8AMATa ura3-52 his3-11,15 leu2-3,112
FSO4-8BMATa ura3-52 his3-11,15 leu2-3,112 rho5::kanMX
HCLO01-3BMATa ura3-52 his3-11,15 leu2-3,112 GFP-LMO1-KlLEU2
HCLO01-8BMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
HCLO01-15CMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
GFP-LMO1-KlLEU2
HCLO01-23DMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD] GFP-LMO1-KlLEU2
HCLO01-27DMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
HCSO20MATalpha ura3-52 his3-11,5 leu2-3,112 rho5::kanMX
DCK1-3GFP-SkHIS3
HCSO25MATa ura3-52 his3-11,5 leu2-3,112 rho5::kanMX LMO1-3GFP-SkHIS3
HCSO26MATalpha ura3-52 his3-11,5 leu2-3,112 lmo1::kanMX
DCK1-3GFP-SkHIS3
HCSO33MATalpha ura3-52 his3-11,5 leu2-3,112 dck1::kanMX
LMO1-3GFP-SkHIS3
HD56-5A/ dck1ΔH1MATalpha ura3-52 his3-11,15 leu2-3,112 dck1::SpHIS5
HD56-5A/ dck1ΔKL6MATalpha ura3-52 his3-11,15 leu2-3,112 dck1::KlLEU2
HFSO83MATalpha ura3-52 his3-11,15 leu2-3,112 rho5:kanMX MID2-RHO5G12V::SkHIS3
HLBO19-1BMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
GFP-LMO1-KlLEU2 IDP1-mCherry-kanMX
HLBO19-3AMATalpha ura3-52 his3-11,5 leu2-3,112 GFP-LMO1-KlLEU2
HLBO19-3BMATa ura3-52 his3-11,5 leu2-3,112 GFP-LMO1-KlLEU2
IDP1-mCherry-kanMX
HLBO19-5AMATalpha ura3-52 his3-11,5 leu2-3,112 GFP-LMO1-KlLEU2
IDP1-mCherry-kanMX
HLBO19-8CMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
GFP-LMO1-KlLEU2 IDP1-mCherry-kanMX
HLBO20-4BMATa ura3-52 his3-11,15 leu2-3,112
HLBO21-2AMATa ura3-52 his3-11,15 leu2-3,112 DCK1-3GFP-SpHIS5
IDP1-mCherry-kanMX
HLBO21-3DMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
DCK1-3GFP-SpHIS5IDP1-mCherry-kanMX
HLBO21-6AMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
DCK1-3GFP-SpHIS5IDP1-mCherry-kanMX
HLBO21-10AMATalpha ura3-52 his3-11,15 leu2-3,112 DCK1-3GFP-SpHIS5
IDP1-mCherry-kanMX
HLBO22-2AMATa ura3-52 his3-11,15 leu2-3,112 DCK1-3GFP-SpHIS5
HLBO22-2DMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
HLBO22-3BMATalpha ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
DCK1-3GFP-SpHIS5
HLBO22-4DMATalpha ura3-52 his3-11,15 leu2-3,112 DCK1-3GFP-SpHIS5
HLBO22-5BMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
DCK1-3GFP-SpHIS5
HLBO22-9DMATa ura3-52 his3-11,15 leu2-3,112::pLA012 [GB-FIS1TMD]
HOD464-7BMATa ura3-52 his3-11,15 leu2-3,112 lmo1::kanMX
HOD500-10AMATa ura3-52 leu2-3,113 his3-11,15 rho5::kanMX MID2-RHO5-SkHIS3
HOD500-11BMATalpha ura3-52 leu2-3,113 his3-11,15 rho5::kanMX
MID2-RHO5-SkHIS3
HOD512-3AMATa ura3-52 his3-11,15 leu2-3,112 MID2-GFP-RHO5-SkHIS3
HOD529-2BMATa ura3-52 his3-11,15 leu2-3,112::pJJH3096 [LEU2-RHO5G12V-FIS1TMD] rho5::kanMX
HOD529-5CMATalpha ura3-52 his3-11,15 leu2-3,112::pJJH3096 [LEU2-RHO5G12V-FIS1TMD] rho5::kanMX
LBO81MATa ura3-52 his3-11,15 leu2-3,112 lmo1::KlURA3
LBO130MATalpha ura3-52 his3-11,15 leu2-3,112::pJJH3024 [RHO5-FIS1TMD] rho5::kanMX
LBO132MATa ura3-52 his3-11,15 leu2-3,112::pJJH3024 [RHO5-FIS1TMD] rho5::kanMX
1 All strains employed are segregants from isogenic crosses derived from DHD5 [39].
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Bischof, L.; Schweitzer, F.; Sterk, C.C.; Heinisch, J.J. The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response. Int. J. Mol. Sci. 2022, 23, 7896. https://doi.org/10.3390/ijms23147896

AMA Style

Bischof L, Schweitzer F, Sterk CC, Heinisch JJ. The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response. International Journal of Molecular Sciences. 2022; 23(14):7896. https://doi.org/10.3390/ijms23147896

Chicago/Turabian Style

Bischof, Linnet, Franziska Schweitzer, Carolin C. Sterk, and Jürgen J. Heinisch. 2022. "The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response" International Journal of Molecular Sciences 23, no. 14: 7896. https://doi.org/10.3390/ijms23147896

APA Style

Bischof, L., Schweitzer, F., Sterk, C. C., & Heinisch, J. J. (2022). The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response. International Journal of Molecular Sciences, 23(14), 7896. https://doi.org/10.3390/ijms23147896

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