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Article

OsWRKY114 Inhibits ABA-Induced Susceptibility to Xanthomonas oryzae pv. oryzae in Rice

by
Seungmin Son
1,†,
Jong Hee Im
1,2,†,
Giha Song
1,
Suhyeon Nam
1,3 and
Sang Ryeol Park
1,*
1
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2
Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA
3
Department of Crop Science & Biotechnology, Jeonbuk National University, Jeonju 54896, Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(15), 8825; https://doi.org/10.3390/ijms23158825
Submission received: 18 July 2022 / Revised: 4 August 2022 / Accepted: 5 August 2022 / Published: 8 August 2022
(This article belongs to the Special Issue Plant Disease Resistance 2.0)
Browse Figures
Versions Notes

Abstract

:
The phytohormone abscisic acid (ABA) regulates various aspects of plant growth, development, and stress responses. ABA suppresses innate immunity to Xanthomonas oryzae pv. oryzae (Xoo) in rice (Oryza sativa), but the identity of the underlying regulator is unknown. In this study, we revealed that OsWRKY114 is involved in the ABA response during Xoo infection. ABA-induced susceptibility to Xoo was reduced in OsWRKY114-overexpressing rice plants. OsWRKY114 attenuated the negative effect of ABA on salicylic acid-dependent immunity. Furthermore, OsWRKY114 decreased the transcript levels of ABA-associated genes involved in ABA response and biosynthesis. Moreover, the endogenous ABA level was lower in OsWRKY114-overexpressing plants than in the wild-type plants after Xoo inoculation. Taken together, our results suggest that OsWRKY114 is a negative regulator of ABA that confers susceptibility to Xoo in rice.

1. Introduction

Phytohormones, such as abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), ethylene (ET), auxins, brassinosteroids, cytokinins, and gibberellins, play critical roles in various plant processes during growth, development, and stress responses [1,2,3]. ABA is a major phytohormone that regulates embryo maturation, seed dormancy, germination, flowering, and senescence [4]. Moreover, ABA is a key phytohormone that modulates various stress responses in plants [5,6]. Endogenous ABA contents increase in response to abiotic stress (e.g., drought and salt), making ABA an important factor in a plant’s ability to overcome harsh environmental conditions [7,8]. ABA is also involved in biotic stress responses: ABA inhibits pathogen entry by inducing stomatal closure and increases plant disease resistance [9,10,11]. However, ABA commonly suppresses the major biotic stress-related phytohormone SA and is thus a negative regulator of plant innate immunity [12]. Therefore, ABA can increase the susceptibility of a wide variety of pathogens [13,14,15,16,17].
Indeed, exogenous application of ABA or the accumulation of endogenous ABA causes an enhanced susceptibility to bacterial and fungal pathogens in various plants [18,19,20,21,22,23]. For example, exogenous ABA increases the susceptibility of biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000, and the bacterial effector AvrPtoB induces the expression of gene encoding 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), a key enzyme in pathogen-induced ABA biosynthesis, to suppress plant defense response in Arabidopsis (Arabidopsis thaliana) [24]. Furthermore, ABA and the overexpression of NCED genes (i.e., NCED3 and NCED5) increase the growth of the P. syringae strains in Arabidopsis [25].
The crosstalk between ABA and three major defense phytohormones, such as SA, JA, and ET, is important for plant resistance during disease development. ABA has an antagonistic interaction with SA signaling that confers resistance to biotrophic pathogens, while it can act antagonistically or synergically with JA/ET signaling involved in resistance to necrotrophic pathogens [5,26,27]. Therefore, ABA-mediated SA suppression plays a critical role in the susceptibility of biotrophic pathogens. In Arabidopsis, ABA decreases the expression of isochorismate synthase 1 (ICS1), a key enzyme in SA biosynthesis, and plays a central role in the attenuation of the SA-mediated plant defense mechanism against Pst DC3000 [28]. ABA and NCEDs increase the susceptibility of the P. syringae strains through antagonistic relationship with SA, while ABA promotes JA synthesis [25]. The pyrabactin-resistance 1/pyrabactin-resistance-like/regulatory component of the ABA receptor (PYR/PYL/RCAR), such as PYR1, compromises SA defense signaling against Pst DC3000, while it enhances ET signaling [27]. In rice (Oryza sativa), ABA also increases the susceptibility of the biotrophic bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and inhibits SA-mediated defense response [29]. However, the signaling components that regulate ABA-induced pathogen susceptibility remain largely elusive.
The plant-specific WRKY transcription factor family plays substantial roles in plant growth, development, and biotic/abiotic stress responses [30]. WRKY transcription factors comprising a large number of members are classified by one or two 60-amino acid WRKY domains (WDs), which consist highly conserved WRKY motif [WRKYGQK] and zinc finger-like motif [C2H2 or C2HC] (i.e., Group I, two WDs; Group II, one WD with C2H2 zinc finger; and Group III, one WD with C2HC zinc finger) [31]. They directly bind to W-box motif (TTGACC/T) in promoter regions of target genes and regulate the expression of them [30]. WRKYs modulate various signaling cascades as either activators or repressors [31,32,33,34,35]. In rice, there are 103 genes encoding WRKY transcription factors, including 28 Group III members [36,37]. Recent studies have indicated that several rice WRKYs are associated with ABA. For instance, OsWRKY5 increases ABA-induced leaf senescence by upregulating ABA biosynthetic genes and raising endogenous ABA level [38]. OsWRKY29 negatively regulates ABA signaling and decreases the expression of several ABA-related genes during seed dormancy [39]. OsWRKY50 inhibits ABA-dependent seed germination and seedling growth, while enhancing salt stress tolerance via an ABA-independent pathway [40]. However, whether any OsWRKYs regulate ABA signaling during biotic stress is unknown. We previously revealed that OsWRKY114 enhances innate immunity to Xoo through the direct upregulation of pathogenesis-related (PR) genes, such as OsPR1a and chitinase [41]. Moreover, most recently, it was reported that OsWRKY114 decreases the expressions of PYR/PYL/RCAR genes that improve drought tolerance through stomatal closure [42]. However, the biological function of OsWRKY114 in ABA-pathogenesis has yet to be elucidated. Here, we established a molecular mechanism for how OsWRKY114 inhibits ABA-mediated susceptibility in Xoo infection.

2. Results

2.1. OsWRKY114 Expression Is Modulated by ABA

To explore the expression patterns of OsWRKY114, we analyzed a public expression database of Genevestigator. OsWRKY114 expression is affected by various biotic/abiotic stresses and ABA (Figure 1A). Since ABA is an important phytohormone involved in various stress responses including Xoo and drought, we sought to independently confirm the effects of ABA on OsWRKY114 expression. To this end, we sprayed 4-week-old rice plants (from the japonica cultivar Ilmi) with a 100 µM ABA solution and collected samples at various times for reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. The OsWRKY114 transcript level slightly increased 2 h (hour) after exogenous ABA treatment, but it rapidly reverted to a basal level (Figure 1B). Since OsWRKY114 is involved in disease resistance to Xoo [41], we examined whether ABA affects the OsWRKY114 transcript level during Xoo infection. Accordingly, we pretreated the rice cultivar Ilmi with 100 µM ABA or mock (0.02% [v/v] Tween 20) solution, and then inoculated the plants with the compatible Xoo strain KACC10859 three days later, as previously described [29]. Interestingly, OsWRKY114 transcript level was lower in ABA-pretreated plants than in mock-pretreated plants after Xoo inoculation (Figure 1C). This result suggests that OsWRKY114 is negatively regulated by ABA during Xoo infection.

2.2. OsWRKY114 Alleviates ABA-Dependent Downregulation of Basal Defense Genes during Xoo Infection

To confirm that OsWRKY114 is associated with the ABA response during Xoo infection, we measured the transcript levels of PR genes, such as OsPR1a and chitinase, whose expression is activated directly by OsWRKY114. Rice plants pretreated with ABA exhibited lower OsPR1a and chitinase transcript levels at 12 h after compatible Xoo inoculation, compared to the mock-treated plants (Figure 2A,B). The expression levels of OsPR1a and chitinase were reduced according to the same pattern of the OsWRKY114 transcript level upon ABA treatment. However, OsPR1a and chitinase transcript levels were not significantly reduced by pretreatment with ABA in transgenic rice plants overexpressing OsWRKY114 (OsWRKY114OX) (Figure 2A,B). These results suggest that the inhibition of OsWRKY114 by ABA increases the susceptibility to Xoo.

2.3. ABA-Induced Susceptibility to Xoo Is Repressed in OsWRKY114-Overexpressing Plants

To investigate the role of OsWRKY114 in ABA-mediated susceptibility to Xoo, we carried out a disease assay in OsWRKY114OX and wild-type plants. We pretreated 4-week-old rice plants with 100 µM ABA or a mock solution, and inoculated them with Xoo 3 days later. The susceptibility of Xoo increased in all ABA-treated plants compared to their mock-treated controls (Figure 3A,B). Importantly, the length of ABA-induced lesions was almost 50% less in OsWRKY114OX plants than in the wild-type plants (Figure 3C). To further confirm the correlation between OsWRKY114 and ABA during Xoo infection, we inoculated it in 4-week-old rice plants pretreated with the ABA biosynthesis inhibitor fluridone (FLU). Although the susceptibility of Xoo was decreased significantly in FLU-treated wild-type plants compared to their mock-treated controls, it was not in OsWRKY114OX lines (Figure 3D,E). This result suggests that the Xoo-induced ABA biosynthesis is inhibited effectively in OsWRKY114OX plants. Taken together, these results suggest that OsWRKY114 is a negative regulator of ABA-induced susceptibility to Xoo.

2.4. The Negative Effect of ABA on SA Defense Mechanism Is Weaker in OsWRKY114-Overexpressing Plants

The expressions of SA marker genes were suppressed at 4 days after compatible Xoo inoculation in the ABA-treated rice, but were not suppressed in mock-treated rice [29]. We thus determined the transcript levels of OsNPR1 and OsWRKY45 at 4 days after Xoo inoculation in OsWRKY114OX and wild-type plants. ABA pretreatment lowered OsNPR1 and OsWRKY45 transcript levels sharply in the wild-type plants; notably, this ABA response was attenuated in OsWRKY114OX plants after Xoo inoculation (Figure 4A,B). However, the two genes were expressed to similar levels across all genotypes in mock-treated plants after Xoo inoculation (Figure 4A,B). To clarify the role of OsWRKY114 in SA signaling, we performed the transient protoplast transfection assay to test whether OsWRKY114 might directly regulate the expressions of OsNPR1 and OsWRKY45. We individually introduced the constructs pEarleyGate104/OsWRKY114 (overexpression) and pB7GWIWG(II)/OsWRKY114-RNAi (RNA interference [RNAi]) into rice protoplasts and collected samples after 8 h for RT-qPCR (Supplementary Figure S1A). The transient overexpression of OsWRKY114 failed to lead to a significant increase in OsNPR1 and OsWRKY45 transcript levels, while OsPR1a was upregulated as expected (Supplementary Figure S1B). Similarly, silencing of OsWRKY114 did not affect OsNPR1 and OsWRKY45 transcript levels.
To dissect the feature of OsWRKY114 in ABA and SA crosstalk, we treated the phytohormones to OsWRKY114OX and wild-type plants and measured the gene expression of OsWRKY45. The gene expression of OsWRKY45 was increased by SA but the expression was reduced by SA and ABA co-treatment (Figure 4C). However, the reduction was alleviated in OsWRKY114OX (Figure 4C). To examine the consequences of OsWRKY114 overexpression and silencing, we used a protoplast transient transfection system. SA dependently increased OsWRKY45 was reduced by ABA co-treatment, and the reduction was alleviated in OsWRKY114-expressed protoplast, such as in OsWRKY114OX (Figure 4D), but had a more reduced OsWRKY45 expression in OsWRKY114-RNAi-expressed protoplast (Figure 4D). These results suggest that OsWRKY114 does not directly regulate SA signaling, but indirectly enhances SA-dependent disease resistance by inhibiting ABA response after Xoo infection.

2.5. Various ABA-Response and ABA-Related Genes Are Downregulated in OsWRKY114-Overexpressing Plants

To explore the regulatory mechanism of OsWRKY114 in the ABA response, we analyzed the transcript levels of various genes associated with ABA by RT-qPCR analysis. We observed that the ABA-response genes OsABI5 (ABA-insensitive 5), OsVP1 (Viviparous 1), TRAB1 (Transcription factor responsible for ABA regulation 1), OsbZIP23 (Basic leucine zipper 23), and OsbZIP72 are expressed at lower levels in OsWRKY114OX plants compared to the wild-type plants (Figure 5A). Moreover, we confirmed the repression of ABA response by OsWRKY114, as evidenced by the downregulation of the ABA-related genes OsLEA3-1 (Late embryogenesis abundant 3-1), OsLEA3-2, OsLEA4, OsLEA5, and OsEm1 (Embryonic abundant protein 1) (Figure 5B). These results indicate that OsWRKY114 downregulates the expressions of ABA-associated genes.

2.6. ABA Biosynthesis Is Attenuated in OsWRKY114-Overexpressing Plants during Xoo Infection

To determine whether OsWRKY114 is involved in ABA biosynthesis, we monitored the transcript levels of ABA metabolism genes. OsNCED genes, such as OsNCED3 and OsNCED4, appeared to be downregulated in OsWRKY114OX plants relative to the wild-type plants (Figure 6A). OsNCEDs play a critical role in ABA biosynthesis [43,44], prompting us to measure ABA contents in OsWRKY114OX and wild-type plants. The endogenous ABA level was only slightly lower in OsWRKY114OX plants compared to wild-type plants (Figure 6B). However, 8 days after Xoo inoculation, the endogenous ABA level increased by approximately 50% in wild-type plants relative to mock-treated plants, but it showed no significant differences in OsWRKY114OX plants (Figure 6B). These results indicate that OsWRKY114 significantly attenuates ABA biosynthesis after Xoo infection.

3. Discussion

In previous our study, the OsWRKY114 directly activates the promoters of PR genes and enhances disease resistance to Xoo [41]. Furthermore, interestingly, drought tolerance is reduced in OsWRKY114OX plants [42]. ABA is the central phytohormone conferring plant tolerance against abiotic stress including drought, whereas it reduces disease resistance to biotrophic pathogens via an antagonistic effect of SA signaling [45]. Therefore, these results suggest that OsWRKY114 may be a negative regulator of ABA. However, the regulation of ABA by OsWRKY114 during Xoo infection is not yet established. Here, we demonstrate that OsWRKY114 inhibits ABA-dependent susceptibility during Xoo infection.
First, we analyzed the expression pattern of OsWRKY114 and PR genes (i.e., OsPR1a and chitinase), which revealed that they are downregulated by ABA after Xoo inoculation (Figure 1C and Figure 2). To better understand the connection between OsWRKY114 and ABA responses during the rice–Xoo interaction, we determined how the OsWRKY114-overexpressing transgenic plants respond to ABA and Xoo infection. Notably, the greater susceptibility to Xoo induced by ABA treatment was less pronounced in OsWRKY114OX plants, based on relative lesion length (Figure 3C). In addition, we discovered that OsWRKY114 increases SA-dependent disease resistance by inhibiting ABA response after Xoo inoculation (Figure 4).
The antagonistic relationship between ABA and SA signaling pathways increases the susceptibility of rice toward Xoo [29], but the role of OsWRKY114 in the relationship is not yet identified. The master regulator of SA signaling, OsNPR1, enhances innate immunity to Xoo [46,47,48]. OsWRKY45, a key regulator of SA signaling, also improves resistance to Xoo [49]. Consistent with the increased resistance to Xoo observed in OsWRKY114OX plants, exogenous application of ABA was accompanied with much lower OsNPR1 and OsWRKY45 transcript levels in the wild-type plants after Xoo inoculation, while this drop was greatly attenuated in OsWRKY114OX plants (Figure 4A,B). However, OsWRKY114 did not appear to directly regulate the transcription of OsNPR1 or OsWRKY45 (Figure 4). Therefore, we concluded that the higher expression levels of SA marker genes (i.e., OsNPR1 and OsWRKY45) in OsWRKY114OX plants treated with ABA reflect a suppression of ABA signaling.
Indeed, various ABA-response and ABA-related genes were downregulated in OsWRKY114OX plants compared to the wild-type plants (Figure 5A,B). Moreover, the ABA biosynthetic genes OsNCED3 and OsNCED4 were expressed at lower levels upon OsWRKY114 overexpression in stable transgenic plants (Figure 6A). NCED enzymes convert 9-cis-violaxanthin or 9-cis-neoxanthin to the ABA precursor xanthoxin [50], such that a higher expression of NCED genes leads to a greater accumulation of ABA in plants [51,52,53]. Especially, OsNCED3 and OsNCED4 were expected to be mainly involved in ABA-induced susceptibility to Xoo [29]. Therefore, the downregulation of OsNCED3 and OsNCED4 by OsWRKY114 suggests that it plays an important role in ABA biosynthesis during Xoo infection. Indeed, Xoo-induced ABA accumulation was attenuated in OsWRKY114OX plants (Figure 6B). These results suggest that OsWRKY114 negatively regulates ABA-induced susceptibility to Xoo by repressing ABA biosynthesis. Moreover, in spite of no significant differences of ABA contents between 4-week-old OsWRKY114OX and wild-type plants (Figure 6B), the expressions of various ABA-associated genes were reduced significantly in OsWRKY114OX plants compared to wild-type plants (Figure 5). These results reveal that OsWRKY114 can also inhibit ABA response.
The WRKY Group II transcription factor OsWRKY11 increase plant resistance to both Xoo and drought through regulating the expression of biotic and abiotic stress-related genes [54]. However, the WRKY Group III transcription factor OsWRKY45 enhances disease resistance to Xoo but reduces drought tolerance in rice [49,55]. Here, we demonstrate the WRKY Group III transcription factor OsWRKY114 increases resistance to Xoo with dual regulation of PR genes and ABA (Figure 7), while it reduces drought tolerance [42]. The WRKY transcription factors belonging to Group III are known to be the most highly evolved WRKYs [56] and they are involved in mainly innate immunity against various pathogens [57,58,59]. Indeed, OsWRKY45 confers broad-spectrum resistance to various pathogens [49,60,61]. Therefore, OsWRKY114 may also be associated with disease resistance to various pathogens.
In conclusion, the dual function of OsWRKY114 in regulating the expression of PR genes and the ABA signaling is important for innate immunity to Xoo (Figure 7). OsWRKY114 not only suppressed ABA responses but also reduced pathogen-induced ABA biosynthesis, leading to increased resistance to Xoo in rice. However, OsWRKY114 is a transcriptional activator, not repressor [41]. Therefore, a detailed mechanism by which OsWRKY114 negatively regulates ABA response and biosynthesis during Xoo infection should be clarified in future studies. Our findings provide valuable information concerning plant–pathogen interactions that may be applicable to plant breeding.

4. Materials and Methods

4.1. Plant Material and Growth Conditions

The Oryza sativa L. japonica rice cultivar ‘Ilmi’ was used as the wild-type plant in this study. OsWRKY114-overexpressing rice plant lines were previously generated and confirmed [41]. All seeds were surface sterilized with a 5% sodium hypochlorite solution and then rinsed thoroughly with sterilized distilled water. The seeds were germinated in sterilized distilled water for 5 days and then transferred to soil or half-strength Murashige and Skoog (MS) medium and grown under a 16 h light and 8 h dark photoperiod at 28 °C.

4.2. Gene Expression Analysis

Expression profiling analysis was performed using Genevestigator (https://genevestigator.com/, (accessed on 11 January 2021)). For RT-qPCR analysis, leaves of rice plants were harvested and frozen in liquid nitrogen and then total RNA was extracted with TRIzol reagent (Invitrogen, Waltham, MA, USA). For each sample, 2 µg total RNA was reverse-transcribed to first-strand cDNA using Superscript III reverse transcriptase (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions. RT-qPCR was performed with gene-specific primers (Supplementary Table S1) on the QuantaStudio 3 PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using SYBR Green Master Mix (Enzynomics, Daejeon, Korea) under the following conditions: 40 cycles of denaturation at 95 °C for 10 s, annealing at 58 °C for 15 s, and extension at 72 °C for 30 s. Gene expression was quantified using the comparative Ct method. OsActin was used as an internal control to determine gene expression.

4.3. Phytohormone and Chemical Treatments

Plants were treated with ABA, SA, and FLU as previously described [29]. Briefly, the ABA (Sigma, St. Louis, MO, USA) concentration was adjusted to 100 µM in 0.02% (v/v) Tween 20, while the FLU (Sigma, St. Louis, MO, USA) concentration was adjusted to 10 µM. The ABA or FLU solution was then sprayed onto 4-week-old rice plants. Mock-treated plants were sprayed with 0.02% (v/v) Tween 20. For phytohormone crosstalk experiments, rice leaf segments were incubated for 8 h in the solution containing 500 µM SA (Sigma, St. Louis, MO, USA) with or without 50 µM ABA.

4.4. Pathogen Inoculation and Disease Assay

Pathogen inoculation and disease assay were performed as previously described with slight modifications [62]. Briefly, rice plants were grown on soil for 4 weeks, and then sprayed with ABA or mock solution. Three days later, the sprayed plants were inoculated with the compatible Xoo strain KACC10859 by the leaf-clipping method. The length of disease lesions was measured at 14 days post-inoculation. The length of the ABA-induced lesions was calculated according to the following equation: ABA-induced lesion length (%) = ([Lesion length of ABA-pretreated plants] − [Lesion length of mock-pretreated plants]) × 100.

4.5. Transient Gene Expression Assay in Protoplasts

pEarleyGate104/OsWRKY114 and pB7GWIWG(II)/OsWRKY114-RNAi constructs were previously generated [41]. Transient protoplast transfection and phytohormone treatment were performed as previously described [63] with a slight modification. Briefly, rice seedlings were grown on half-strength MS medium for 2 weeks. Protoplasts were isolated and the constructs were individually transfected by the polyethylene glycol (PEG)-mediated transfection method. The transfected protoplasts were incubated in WI solution at 28 °C. For phytohormone treatment, 500 mM SA with or without 50 µM ABA was added to the solution after 1 h of incubation. After 8 h incubation, the protoplasts were collected for total RNA extraction.

4.6. Analysis of ABA Contents

ABA contents were determined as previously described [64]. Briefly, around 5 g of 4-week-old rice leaves were ground to powder with a mortar and pestle in liquid nitrogen. The powder was homogenized with 20 mL of 80% (v/v) methanol for 30 min on ice. The samples were filtered onto two layers of Miracloth and the solution evaporated under vacuum. The residue was dissolved in 0.5 M phosphate buffer (pH 8.0) with gentle stirring for 30 min. After centrifugation, the supernatant was discarded and the pellet was washed with 20 mL of mineral spirit. The pH was adjusted to 2.8 with 12 M hydrochloric acid. The samples were centrifuged again and resuspended in 10 mL ethyl acetate. This step was repeated three more times and the supernatants pooled. The extracts were lyophilized and dissolved in 5 mL of 0.5 M phosphate buffer (pH 8.0). The samples were purified on a Sephadex® G-10 column (Sigma, St. Louis, MO, USA). The eluates were lyophilized and dissolved in 1 mL acetonitrile and analyzed by HPLC (Chromaster, Hitachi, Japan) (+)-ABA was purchased from Sigma (Sigma, St. Louis, MO, USA) and used as standard.

4.7. Statistical Analysis

All experiments were independently conducted at least three times, and the average values from the independent experiments were presented. The data were analyzed by t-test or ANOVA. Asterisks denote significant differences (* p < 0.05, ** p < 0.01) and different letters indicate statistical differences (p < 0.05).

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ijms23158825/s1.

Author Contributions

Conceptualization, S.S. and J.H.I.; methodology, S.S. and J.H.I.; software, S.S. and J.H.I.; validation, S.S. and J.H.I.; formal analysis, S.S. and J.H.I.; investigation, S.S., J.H.I., G.S., and S.N.; resources, S.S. and J.H.I.; data curation, S.S. and J.H.I.; writing—original draft preparation, S.S.; writing—review and editing, S.S.; visualization, S.S.; supervision, S.R.P.; project administration, S.R.P.; funding acquisition, S.R.P. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Research Program for Agricultural Science and Technology Development (Project No. PJ01570601) and supported by the 2022 Fellowship Program (Project No. PJ01661001 and PJ01570601) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in the article or the Supplementary Materials.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. ABA modulates the transcription of OsWRKY114 during Xoo inoculation. (A) Expression profile of OsWRKY114 under various stress conditions. Gene expression data were obtained from Genevestigator. (B) Relative OsWRKY114 transcript level after ABA treatment, as determined by RT-qPCR. Four-week-old rice plants were sprayed with 100 µM ABA and incubated for the indicated times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± standard deviation (SD). * p < 0.05 by t-test relative to the 0 h sample. (C) Relative OsWRKY114 transcript level in ABA-treated plants after Xanthomonas oryzae pv. oryzae inoculation. Four-week-old rice plants were pretreated with 100 µM ABA or mock (0.2% [v/v] Tween 20) solution. Three days later, leaves were inoculated with Xoo and collected after the indicated incubation times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to the 0 h mock-treated sample and between the indicated comparing samples.
Figure 1. ABA modulates the transcription of OsWRKY114 during Xoo inoculation. (A) Expression profile of OsWRKY114 under various stress conditions. Gene expression data were obtained from Genevestigator. (B) Relative OsWRKY114 transcript level after ABA treatment, as determined by RT-qPCR. Four-week-old rice plants were sprayed with 100 µM ABA and incubated for the indicated times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± standard deviation (SD). * p < 0.05 by t-test relative to the 0 h sample. (C) Relative OsWRKY114 transcript level in ABA-treated plants after Xanthomonas oryzae pv. oryzae inoculation. Four-week-old rice plants were pretreated with 100 µM ABA or mock (0.2% [v/v] Tween 20) solution. Three days later, leaves were inoculated with Xoo and collected after the indicated incubation times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to the 0 h mock-treated sample and between the indicated comparing samples.
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Figure 2. The expression levels of PR genes regulated by OsWRKY114 are not suppressed by ABA in OsWRKY114-overexpressing plants during Xanthomonas oryzae pv. oryzae infection. (A,B) Relative transcript levels for OsPR1a (A) and chitinase (B), as determined by RT-qPCR. Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xoo and collected after the indicated incubation times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to the 0 h mock-treated wild-type sample and between the indicated comparing samples.
Figure 2. The expression levels of PR genes regulated by OsWRKY114 are not suppressed by ABA in OsWRKY114-overexpressing plants during Xanthomonas oryzae pv. oryzae infection. (A,B) Relative transcript levels for OsPR1a (A) and chitinase (B), as determined by RT-qPCR. Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xoo and collected after the indicated incubation times. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to the 0 h mock-treated wild-type sample and between the indicated comparing samples.
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Figure 3. Overexpression of OsWRKY114 suppresses ABA-induced susceptibility to Xanthomonas oryzae pv. oryzae. (A–C) Bacterial blight disease of ABA- or mock-treated rice plants. Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xoo. (A) Representative images of lesions captured 14 days after inoculation. (B) Lesion length across genotypes and treatments. (C) ABA-induced lesion length across genotypes according to the following equation: ABA-induced lesion length (%) = ([Lesion length of ABA-pretreated plants] − [Lesion length of mock-pretreated plants]) × 100. The red arrow indicates the end of the lesion length. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05). (D,E) Bacterial blight disease of FLU- or mock-treated rice plants. Four-week-old rice plants were pretreated with 10 µM FLU or mock solution. Three days later, leaves were inoculated with Xoo. (D) Representative images of lesions were captured 14 days after inoculation. (E) Lesion length across genotypes and treatments. The red arrow indicates the end of the lesion length. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05).
Figure 3. Overexpression of OsWRKY114 suppresses ABA-induced susceptibility to Xanthomonas oryzae pv. oryzae. (A–C) Bacterial blight disease of ABA- or mock-treated rice plants. Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xoo. (A) Representative images of lesions captured 14 days after inoculation. (B) Lesion length across genotypes and treatments. (C) ABA-induced lesion length across genotypes according to the following equation: ABA-induced lesion length (%) = ([Lesion length of ABA-pretreated plants] − [Lesion length of mock-pretreated plants]) × 100. The red arrow indicates the end of the lesion length. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05). (D,E) Bacterial blight disease of FLU- or mock-treated rice plants. Four-week-old rice plants were pretreated with 10 µM FLU or mock solution. Three days later, leaves were inoculated with Xoo. (D) Representative images of lesions were captured 14 days after inoculation. (E) Lesion length across genotypes and treatments. The red arrow indicates the end of the lesion length. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05).
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Figure 4. ABA-induced SA signaling suppression is alleviated by OsWRKY114. (A,B) Relative transcript levels for OsNPR1 (A) and OsWRKY45 (B). Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xanthomonas oryzae pv. oryzae. Samples were collected 4 days later for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to mock-treated wild-type sample and between the indicated comparing samples. (C) Gene expression OsWRKY45 in SA- or SA+ABA-treated OsWRKY114OX and wild-type plants. A total of 500 µM SA was applied to leaf segments of OsWRKY114OX and wild-type plants alone or with 50 µM ABA. After 8 h of incubation, total RNA was isolated for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to non-treated wild-type sample and between the indicated comparing samples. (D) Gene expression of OsWRKY45 in SA- or SA+ABA-treated protoplasts. Rice protoplasts were transfected with or without constructs pEarleyGate104/OsWRKY114 and pB7GWIWG(II)/OsWRKY114-RNAi, respectively, and then 500 µM SA was treated to protoplasts alone or with 50 µM ABA. After 8 h of incubation, total RNA was isolated for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to non-treated control sample and between the indicated comparing samples.
Figure 4. ABA-induced SA signaling suppression is alleviated by OsWRKY114. (A,B) Relative transcript levels for OsNPR1 (A) and OsWRKY45 (B). Four-week-old rice plants were pretreated with 100 µM ABA or mock solution. Three days later, leaves were inoculated with Xanthomonas oryzae pv. oryzae. Samples were collected 4 days later for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to mock-treated wild-type sample and between the indicated comparing samples. (C) Gene expression OsWRKY45 in SA- or SA+ABA-treated OsWRKY114OX and wild-type plants. A total of 500 µM SA was applied to leaf segments of OsWRKY114OX and wild-type plants alone or with 50 µM ABA. After 8 h of incubation, total RNA was isolated for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to non-treated wild-type sample and between the indicated comparing samples. (D) Gene expression of OsWRKY45 in SA- or SA+ABA-treated protoplasts. Rice protoplasts were transfected with or without constructs pEarleyGate104/OsWRKY114 and pB7GWIWG(II)/OsWRKY114-RNAi, respectively, and then 500 µM SA was treated to protoplasts alone or with 50 µM ABA. After 8 h of incubation, total RNA was isolated for RT-qPCR analysis. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to non-treated control sample and between the indicated comparing samples.
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Figure 5. The expressions of various ABA-associated genes are downregulated in OsWRKY114-overexpressing plants. (A) Relative transcript levels of ABA-response genes in 4-week-old rice plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to wild-type sample. (B) Relative transcript levels of ABA-related genes in 4-week-old rice plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to wild-type sample.
Figure 5. The expressions of various ABA-associated genes are downregulated in OsWRKY114-overexpressing plants. (A) Relative transcript levels of ABA-response genes in 4-week-old rice plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to wild-type sample. (B) Relative transcript levels of ABA-related genes in 4-week-old rice plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, determined by t-test relative to wild-type sample.
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Figure 6. ABA biosynthesis is attenuated in OsWRKY114-overexpressing plants after Xanthomonas oryzae pv. oryzae inoculation. (A) Relative OsNCEDs transcript levels in 4-week-old OsWRKY114-overexpressing and wild-type plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. ** p < 0.01, determined by t-test relative to wild-type sample. (B) Analysis of ABA contents in 4-week-old OsWRKY114-overexpressing and wild-type plants. Leaves of 4-week-old rice plants were collected or treated with Xoo or mock solution. After 8 days, leaves of rice plants treated with Xoo- or mock solution were collected to determine endogenous ABA contents. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05).
Figure 6. ABA biosynthesis is attenuated in OsWRKY114-overexpressing plants after Xanthomonas oryzae pv. oryzae inoculation. (A) Relative OsNCEDs transcript levels in 4-week-old OsWRKY114-overexpressing and wild-type plants, as determined by RT-qPCR. cDNA was synthesized from total RNA in rice leaves. OsActin was used as an internal control. Data are shown as means ± SD. ** p < 0.01, determined by t-test relative to wild-type sample. (B) Analysis of ABA contents in 4-week-old OsWRKY114-overexpressing and wild-type plants. Leaves of 4-week-old rice plants were collected or treated with Xoo or mock solution. After 8 days, leaves of rice plants treated with Xoo- or mock solution were collected to determine endogenous ABA contents. Data are shown as means ± SD. Different letters indicate statistical differences according to ANOVA (p < 0.05).
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Figure 7. A working model of OsWRKY114 in innate immunity to Xanthomonas oryzae pv. oryzae. OsWRKY114 enhances disease resistance to Xoo through both upregulation of PR genes and downregulation of the negative effect of ABA on SA-dependent immunity in rice. However, since ABA is a major phytohormone associated with abiotic stresses, the inhibition of ABA signaling by OsWRKY114 can reduce drought tolerance.
Figure 7. A working model of OsWRKY114 in innate immunity to Xanthomonas oryzae pv. oryzae. OsWRKY114 enhances disease resistance to Xoo through both upregulation of PR genes and downregulation of the negative effect of ABA on SA-dependent immunity in rice. However, since ABA is a major phytohormone associated with abiotic stresses, the inhibition of ABA signaling by OsWRKY114 can reduce drought tolerance.
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Son, S.; Im, J.H.; Song, G.; Nam, S.; Park, S.R. OsWRKY114 Inhibits ABA-Induced Susceptibility to Xanthomonas oryzae pv. oryzae in Rice. Int. J. Mol. Sci. 2022, 23, 8825. https://doi.org/10.3390/ijms23158825

AMA Style

Son S, Im JH, Song G, Nam S, Park SR. OsWRKY114 Inhibits ABA-Induced Susceptibility to Xanthomonas oryzae pv. oryzae in Rice. International Journal of Molecular Sciences. 2022; 23(15):8825. https://doi.org/10.3390/ijms23158825

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Son, Seungmin, Jong Hee Im, Giha Song, Suhyeon Nam, and Sang Ryeol Park. 2022. "OsWRKY114 Inhibits ABA-Induced Susceptibility to Xanthomonas oryzae pv. oryzae in Rice" International Journal of Molecular Sciences 23, no. 15: 8825. https://doi.org/10.3390/ijms23158825

APA Style

Son, S., Im, J. H., Song, G., Nam, S., & Park, S. R. (2022). OsWRKY114 Inhibits ABA-Induced Susceptibility to Xanthomonas oryzae pv. oryzae in Rice. International Journal of Molecular Sciences, 23(15), 8825. https://doi.org/10.3390/ijms23158825

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