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Volume 23
Issue 7
10.3390/ijms23073938
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Article
Peer-Review Record

Di-(2-ethylhexyl) Phthalate Triggers Proliferation, Migration, Stemness, and Epithelial–Mesenchymal Transition in Human Endometrial and Endometriotic Epithelial Cells via the Transforming Growth Factor-β/Smad Signaling Pathway

Int. J. Mol. Sci. 2022, 23(7), 3938; https://doi.org/10.3390/ijms23073938
by Hwi Gon Kim 1,†, Ye Seon Lim 2,3,†, Seonyeong Hwang 2,3, Hye-Yoon Kim 2,3, Yuseok Moon 3,4, Yong Jung Song 1, Yong-Jin Na 1 and Sik Yoon 2,3,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2022, 23(7), 3938; https://doi.org/10.3390/ijms23073938
Submission received: 2 March 2022 / Revised: 29 March 2022 / Accepted: 31 March 2022 / Published: 1 April 2022
(This article belongs to the Special Issue Environmental Determinants and Their Impact on Human Reproduction and Reproductive Immunology)

Round 1

Reviewer 1 Report

This is a very interesting piece if work where the authors report DEHP induced proliferation, migration, stemness and EMT in endometrial cells. The authors successfully demonstrated that the activation of TGF-beta/SMAD pathway is responsible for these changes. Overall the paper is scientifically sound and well presented. There are some comments which I believe the authors should address:

 

  1. Figure 1B- 24 and 48 h data. Not a good representation of what has been shown in figure 1A. The panel in 1B does not show any increased proliferation after 48 hour treatment. The figures should be replaced with one that is better representative.
  2. It is good practice to initially use more than 1 drug inhibitor at various concentrations (nm-um). (for information)
  3. Similarly it is important to use 2-3 house keeping genes/proteins in blots and gene expression experiments to ensure validity of the data (for information) 

  4. Very long sentence (52-62). It would be good to mention the type of products and cite a couple of references. There is no need to list each product.

    Some sentences such as 144-146 need to be rewritten. The structure is poor.

 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

I read with great interest the manuscript, which falls within the aim of this Journal. In my honest opinion, the topic is interesting enough to attract the readers’ attention.

Well done. A really well written paper. I only have a small suggestion:

- Introduction part, from line 75 to 81, references are missing.

- It would be appreciable if you can add some theories on etiopathogenesis, citing those about the altered intestinal absorption that can cause transposition of intestinal microbiota and the inflammation of the peritoneal fluid (PMID: 32401078), highlighting the relationships between endometriosis and the host microbiome (PMID: 33851803)

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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