Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice

Kim, Geebum; Lee, So Young; Oh, Seyeon; Jang, Jong-Won; Lee, Jehyuk; Kim, Hyun-Seok; Son, Kuk Hui; Byun, Kyunghee

doi:10.3390/ijms252312522

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice

by

Geebum Kim

^1,†,

So Young Lee

^2,†,

Seyeon Oh

³,

Jong-Won Jang

^3,4,

Jehyuk Lee

^5,6

,

Hyun-Seok Kim

⁷,

Kuk Hui Son

^2,*

and

Kyunghee Byun

^3,4,5,*

¹

Misogain Dermatology Clinic, Gimpo 10108, Republic of Korea

²

Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University, Incheon 21565, Republic of Korea

³

Functional Cellular Networks Laboratory, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea

⁴

Department of Health Sciences and Technology, Gachon Advanced Institute for Health & Sciences and Technology (GAIHST), Gachon University, Incheon 21999, Republic of Korea

⁵

Department of Anatomy & Cell Biology, College of Medicine, Gachon University, Incheon 21936, Republic of Korea

⁶

Doctorbom Clinic, Seoul 06614, Republic of Korea

⁷

Kim Hyun Seok Plastic Surgery Clinic, Seoul 06030, Republic of Korea

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2024, 25(23), 12522; https://doi.org/10.3390/ijms252312522

Submission received: 5 September 2024 / Revised: 15 November 2024 / Accepted: 18 November 2024 / Published: 21 November 2024

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

Download Versions Notes

Abstract

Steroids, which are often used to treat the inflammation associated with various skin diseases, have several negative side effects. As Ecklonia cava extract has anti-inflammatory effects in various diseases, we evaluated the efficacy of Ecklonia cava-derived extracellular vesicles (EVEs) in decreasing 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation. We determined the effect of the EVEs on the TLR4/NF-κB/NLRP3 inflammasome in human keratinocytes and mouse ear skin. TPA-treated human keratinocytes showed an increased expression of TLR4 and its ligands HMGB1 and S100A8. TPA also increased the expression of (1) NF-κB; (2) the NLRP3 inflammasome components NLRP3, ASC, and caspase 1; and (3) the pyroptosis-related factors GSDMD-NT, IL-18, and IL-1β. However, the expression of these molecules decreased in the TPA-treated human keratinocytes after EVE treatment. Similar to the in vitro results, TPA increased the expression of these molecules in mouse ear skin, and EVE treatment decreased their expression. The TPA treatment of skin increased edema, redness, neutrophil infiltration, and epidermal thickness, and EVE reduced these symptoms of inflammation. In conclusion, the EVEs decreased TPA-induced skin inflammation, which was associated with a decrease in the TLR4/NF-κB/NLRP3 inflammasome.

Keywords: Ecklonia cava extract; chronic skin inflammation; NLRP3 inflammasome

Share and Cite

MDPI and ACS Style

Kim, G.; Lee, S.Y.; Oh, S.; Jang, J.-W.; Lee, J.; Kim, H.-S.; Son, K.H.; Byun, K. Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice. Int. J. Mol. Sci. 2024, 25, 12522. https://doi.org/10.3390/ijms252312522

AMA Style

Kim G, Lee SY, Oh S, Jang J-W, Lee J, Kim H-S, Son KH, Byun K. Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice. International Journal of Molecular Sciences. 2024; 25(23):12522. https://doi.org/10.3390/ijms252312522

Chicago/Turabian Style

Kim, Geebum, So Young Lee, Seyeon Oh, Jong-Won Jang, Jehyuk Lee, Hyun-Seok Kim, Kuk Hui Son, and Kyunghee Byun. 2024. "Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice" International Journal of Molecular Sciences 25, no. 23: 12522. https://doi.org/10.3390/ijms252312522

APA Style

Kim, G., Lee, S. Y., Oh, S., Jang, J. -W., Lee, J., Kim, H. -S., Son, K. H., & Byun, K. (2024). Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice. International Journal of Molecular Sciences, 25(23), 12522. https://doi.org/10.3390/ijms252312522

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Anti-Inflammatory Effects of Extracellular Vesicles from Ecklonia cava on 12-O-Tetradecanoylphorbol-13-Acetate-Induced Skin Inflammation in Mice

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI