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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Oncogenic Role of VWA8-AS1, a Long Non-Coding RNA, in Epstein–Barr Virus-Associated Oral Squamous Cell Carcinoma: An Integrative Transcriptome and Functional Analysis

by
Sawarot Srisathaporn
1,2,
Chamsai Pientong
1,2,*,
Chukkris Heawchaiyaphum
1,2,
Thawaree Nukpook
1,2,
Sirinart Aromseree
1,2 and
Tipaya Ekalaksananan
1,2,*
1
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
2
HPV & EBV and Carcinogenesis Research Group, Khon Kaen University, Khon Kaen 40002, Thailand
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(23), 12565; https://doi.org/10.3390/ijms252312565
Submission received: 24 October 2024 / Revised: 16 November 2024 / Accepted: 21 November 2024 / Published: 22 November 2024
(This article belongs to the Section Molecular Oncology)
Versions Notes

Abstract

Dysregulated long non-coding RNA (lncRNA) expression is linked to various cancers and may be influenced by oncogenic Epstein–Barr virus (EBV) infection, a known and detectable risk factor in oral squamous cell carcinoma (OSCC) patients. However, research on the oncogenic role of EBV-induced lncRNAs in OSCC is limited. To identify lncRNA-associated EBV infection and OSCC carcinogenesis, the differential expression of RNA-seq datasets from paired normal adjacent and OSCC tissues, and microarray data from EBV-negative and EBV-positive SCC25 cells, were identified and selected, respectively, for interaction, functional analysis, and CCK-8 cell proliferation, wound healing, and invasion Transwell assays. In OSCC tissues, 6731 differentially expressed lncRNAs were identified when compared to normal tissues from RNA-seq datasets, with 295 linked to EBV-induced OSCC carcinogenesis from microarray datasets. The EBV-induced lncRNA VWA8-AS1 showed significant upregulation in EBV-positive SCC25 cells and EBV-infected adjacent and OSCC tissue samples. VWA8-AS1 potentially promotes OSCC via the lncRNA–miRNA–mRNA axis or direct protein interactions, affecting various cellular processes. Studies in OSCC cell lines revealed that elevated VWA8-AS1 levels enhanced cell migration and invasion. This study demonstrates VWA8-AS1’s contribution to tumor progression and possible interactions with its targets in OSCC, offering insights for future research on functional mechanisms and therapeutic targets in EBV-associated OSCC.
Keywords: long non-coding RNA; oral squamous cell carcinoma; Epstein–Barr virus; VWA8-AS1 long non-coding RNA; oral squamous cell carcinoma; Epstein–Barr virus; VWA8-AS1

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MDPI and ACS Style

Srisathaporn, S.; Pientong, C.; Heawchaiyaphum, C.; Nukpook, T.; Aromseree, S.; Ekalaksananan, T. The Oncogenic Role of VWA8-AS1, a Long Non-Coding RNA, in Epstein–Barr Virus-Associated Oral Squamous Cell Carcinoma: An Integrative Transcriptome and Functional Analysis. Int. J. Mol. Sci. 2024, 25, 12565. https://doi.org/10.3390/ijms252312565

AMA Style

Srisathaporn S, Pientong C, Heawchaiyaphum C, Nukpook T, Aromseree S, Ekalaksananan T. The Oncogenic Role of VWA8-AS1, a Long Non-Coding RNA, in Epstein–Barr Virus-Associated Oral Squamous Cell Carcinoma: An Integrative Transcriptome and Functional Analysis. International Journal of Molecular Sciences. 2024; 25(23):12565. https://doi.org/10.3390/ijms252312565

Chicago/Turabian Style

Srisathaporn, Sawarot, Chamsai Pientong, Chukkris Heawchaiyaphum, Thawaree Nukpook, Sirinart Aromseree, and Tipaya Ekalaksananan. 2024. "The Oncogenic Role of VWA8-AS1, a Long Non-Coding RNA, in Epstein–Barr Virus-Associated Oral Squamous Cell Carcinoma: An Integrative Transcriptome and Functional Analysis" International Journal of Molecular Sciences 25, no. 23: 12565. https://doi.org/10.3390/ijms252312565

APA Style

Srisathaporn, S., Pientong, C., Heawchaiyaphum, C., Nukpook, T., Aromseree, S., & Ekalaksananan, T. (2024). The Oncogenic Role of VWA8-AS1, a Long Non-Coding RNA, in Epstein–Barr Virus-Associated Oral Squamous Cell Carcinoma: An Integrative Transcriptome and Functional Analysis. International Journal of Molecular Sciences, 25(23), 12565. https://doi.org/10.3390/ijms252312565

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