trans-11-(3,4-Dimethoxyphenyl)-2,3,8,9-tetramethoxy-6-oxo-11,12-dihydro-6H-dibenzo[c,h]chromene-12-carboxylic Acid

Abstract

The title compound, trans-11-(3,4-Dimethoxyphenyl)-2,3,8,9-tetramethoxy-6-oxo-11,12-dihydro-6H-dibenzo[c,h]chromene-12-carboxylic acid (4), was synthesized for the first time via a two-step protocol from 3,4-dimethoxyhomophthalic anhydride (1) and 3,4-dimethoxybenzaldehyde (DMBA). In the first step, 1 reacts with DMBA to give trans-3-(3,4-dimethoxyphenyl)-6,7-dimethoxy-1-oxo-3,4-dihydro-1H-2-benzopyran-4-carboxylic acid (2), which further reacts with two additional equivalents of 1 to give 4. Compound 4 was characterized by means of spectral methods—¹H-, ¹³C-, DEPT-135-NMR, and HRMS.

1. Introduction

Homophthalic anhydride (HA) possesses both nucleophilic and electrophilic properties, allowing it to react specifically with various reagents [1,2,3]. Previous research on tandem reactions utilizing HA as a building block has shown that most proceed through detectable and stable intermediates [4,5]; consequently, exploring their reactivity allows us to study the scope and limitations of these domino reactions and the synthesis of novel complex compounds in a controlled manner. A previous article showed that a domino reaction between unsubstituted HA with aromatic aldehydes results in tetracyclic, steroid-like compounds containing dibenzo[c,h]chromene moiety [6]. Such compounds have been shown to possess important bactericidal properties [7,8,9,10] and potential antiestrogen activity [11].

The methoxy group is frequently presented as a fragment in various natural products, and medicinal chemists have increasingly incorporated this functional group into synthetic pharmaceuticals, acknowledging benefits such as its ligand–target binding, physicochemical characteristics, and essential ADME (Absorption, Distribution, Metabolism, and Excretion) properties [12]. In an attempt to find novel compounds with antiestrogenic activity, herein we present for the first time the synthesis of highly methoxy-substituted trans-11-(3,4-Dimethoxyphenyl)-2,3,8,9-tetramethoxy-6-oxo-11,12-dihydro-6H-dibenzo[c,h]chromene-12-carboxylic acid (4).

2. Results and Discussion

The synthesis of 4 was accomplished via a two-step synthetic procedure, as depicted in Scheme 1 and Scheme 2. In the first step (Scheme 1), we obtained trans-3-(3,4-dimethoxyphenyl)-6,7-dimethoxy-1-oxo-3,4-dihydro-1H-2-benzopyran-4-carboxylic acid (2) by reacting 6,7-dimethoxyhomophtalic anhydride (1) with 3,4-dimethoxybenzaldehyde in the presence of 4-N,N-dimethylaminopyridine (DMAP) [13]. This step proceeds smoothly, giving a diastereomeric mixture of cis- and trans-2 in a ratio of 1:4 in favor of trans-2.

After separating it from its cis isomer, we reacted trans-2 with two additional equivalents of 1 (Scheme 2). The initial investigation of this reaction showed that 1 reacts preferably with itself, giving the dimeric compound 3 [1,14]. However, the portion-wise addition of 1 (0.33 equiv. every 20 min) minimizes, to some extent, the self-dimerization reaction, thus allowing the formation of the target compound 4. Notably, the somewhat harsh reaction conditions (100 °C, pyridine) lead to partial demethylation, resulting in a 3–8% compound loss. This phenomenon can be partially elucidated through existing literature [15]. As a result, the yield of compound 4 is additionally reduced; however, it was successfully isolated in pure crystalline form following column chromatography, and its structure was unequivocally confirmed using various NMR techniques (¹H-, ¹³C-, DEPT-135) and mass spectrometry (HRMS), available as Supplementary Materials. The trans configuration of compound 4 was validated by the singlet signals for the methyne protons (ring C) observed in the ¹H-NMR spectrum. According to the Karplus equation [16], this observation suggests a torsion angle between the protons of approximately 80–90°, indicating an antiperiplanar (diaxial) conformation for the bulky substituents (aryl and carboxylate groups). This finding aligns with previously reported data [6].

To summarize, a steroid-like compound with a high degree of methoxy substitution—suggesting improved biological activity—was synthesized from readily available and inexpensive starting materials through a two-step protocol that involved two domino reactions. This research demonstrates the usefulness of homophthalic anhydride in constructing complex molecules with potential medicinal applications, particularly due to the advantageous methoxy group. Further studies on the biological activity of compound 4, especially its potential as an antiestrogen, are currently underway.

3. Materials and Methods

General: All NMR spectra were recorded in DMSO-d6 on a Bruker Avance III HD 500 (Bruker BioSpin GmbH, Rheinstetten, Germany) operating at 500.13 MHz for 1H and 125.76 MHz for 13C. Chemical shifts are reported in ppm. Reactions were monitored by thin-layer chromatography (TLC) on ALUGRAM SIL G/UV254 silica gel aluminum sheets using an Ethyl Acetate/Petroleum Ether (3:2 v/v) eluent. Column chromatography was carried out with a 1:1 Ethyl Acetate/n-heptane mobile phase on silica gel (0.04–0.063 Kieselgel 60) as a stationary phase. High-Resolution Mass Spectra (HRMS) were obtained on a Shimadzu LCMS-9050. All chemicals used in this study were purchased from Sigma-Aldrich (FOT, Sofia, Bulgaria). The organic solvents were of analytical grade. 6,7-Dimethoxy-4H-isochromene-1,3-dione (1) and trans-3-(3,4-dimethoxyphenyl)-6,7-dimethoxy-1-oxoisochroman-4-carboxylic acid (trans-2) were obtained as shown in Scheme 1.

trans-11-(3,4-Dimethoxyphenyl)-2,3,8,9-tetramethoxy-6-oxo-11,12-dihydro-6H-dibenzo[c,h]chromene-12-carboxylic Acid (4)

In total, 0.640 g (1.648 mmol) of trans-3-(3,4-dimethoxyphenyl)-6,7-dimethoxy-1-oxoisochroman-4-carboxylic acid (trans-2) was dissolved in 10 mL of pyridine and the reaction mixture was heated up to 100 °C. Then, 0.732 g (3.296 mmol) of 6,7-dimethoxy-4H-isochromene-1,3-dione (1) was added portion-wise within 2 h (0.33 equiv. per 20 min) and the reaction mixture was left overnight. After cooling, the mixture was diluted with ethyl acetate and washed consecutively with 10% HCl, water, and 5% NaHCO₃. The bicarbonate layer was then acidified (10% HCl) and extracted with ethyl acetate. The organic layer was washed with water to pH~7, dried over Na₂SO₄, and then subjected to column chromatography to yield 110 mg (14%) of trans-4. White solid, ¹H-NMR (500.13 MHz, DMSO) δ 3.54 (3H, s, OMe), 3.59 (3H, s, OMe), 3.68 (3H, s, OMe), 3.76 (3H, s, OMe), 3.80 (3H, s, OMe), 3.82 (3H, s, OMe), 3.85 (1H, s, 12-H), 4.90 (1H, s, 11-H), 6.40 (1H, ArH, dd, ³J_H,H = 8.3, ⁴J_H,H = 2.0), 6.60 (1H, d, ³J_H,H = 8.4 Hz, ArH), 6.89 (1H, s, ArH), 7.00 (1H, s, ArH), 7.00 (1H, d, ⁴J_H,H = 2.0 Hz, ArH), 7.23 (1H, s, ArH), 7,50 (1H, s, ArH), 12.54 (1H, s, COOH); ¹³C-NMR: δ 173.7, 161.0, 155.6, 150.1, 149.6, 149.2, 148.9, 148.2, 147.2, 133.6, 132.8, 125.6, 121.0, 119.3, 114.6, 113.4, 112.2, 112.1, 110.2, 110.1, 105.5, 104.6, 56.5, 56.3, 56.1, 56.1, 55.9, 55.7, 51.6, 39.7 m.p.: 135–137 °C; HRMS (ESI) m/z calculated for [M + H]⁺ C₃₀H₂₉O₁₀⁺: 549.1760, found; [M + H]⁺ 549.17722.