5,5’-Selenobis(1-benzyl-2-oxo-1,2-dihydropyridine-4-carbaldehyde)

Abstract

Selenium compounds have garnered significant attention in the field of medicinal chemistry due to their unique biochemical properties and potential therapeutic applications for different pathologies. In this study, we report the synthesis of a new selenylated bis-pyridone compound using SeO₂ as the source of selenium. Detailed ¹H and ¹³C NMR characterizations and mass spectral analysis are given.

1. Introduction

Selenium compounds have garnered significant attention in the field of medicinal chemistry due to their unique biochemical properties and potential therapeutic applications [1,2]. Selenium, an essential trace element, is crucial for the proper functioning of several enzymes and proteins, particularly those involved in antioxidant defense and redox regulation [3,4]. The incorporation of selenium into organic molecules has led to the development of novel compounds with diverse pharmacological activities, including antifungal, anticancer, antiviral, antimicrobial, and anti-inflammatory effects [5,6,7,8]. These compounds often exhibit enhanced efficacy and selectivity compared to their sulfur or oxygen analogs. As our understanding of selenium’s role in human health deepens, the design and application of selenium-based therapeutics continue to expand, offering promising avenues for the treatment of various diseases.

The synthesis of organoselenium compounds has attracted a lot of interest in organic chemistry due to the unique chemical properties and biological activities of selenated compounds. Selenium, being chemically similar to sulfur, imparts distinct reactivity patterns that make its incorporation into organic molecules particularly valuable in synthetic strategies [9,10]. Gütlich et al. described a reaction between 3- and 3,5-substituted pyrazoles with selenium dioxide proceeds to afford bis(3R,5R0 -1H-pyrazol-4-yl)selenides in high yield (Figure 1) [11]. Sawant et al. showed that mono- and di-selenylated bis-pyrazole derivatives could be obtained from arylated pyrazole in the presence of SeO₂ and DMSO as solvent [12].

Continuing our research into the discovery of new antimycobacterials [13,14,15,16], we isolated an unusual selenium derivative as a by-product in an oxidation reaction. In this work, the synthesis and characterization of this compound is described.

2. Results and Discussion

The synthesis of compound 2 involved the initial preparation of compound 1 (Scheme 1) [17]. Then, compound 1 was reacted with selenium oxide in dioxane at 110 °C to produce aldehyde 2 in 31% yield. During purification, another product was isolated in 29% yield which, on the basis of previous work and by comparison with the NMR of compound 2, we attributed to the structure of the selenylated product 3 (Scheme 1) [11,12]. Unfortunately, attempts to obtain suitable crystals of compound 3 for an X-ray structure determination using different solvents were not successful. Consequently, the structure was elucidated on the basis of ¹H and ¹³C NMR analyses and mass spectrometry (See Supplementary Materials). The mass spectrum in the negative mode of compound 3 showed a signal at m/z 503.0524, matching the theoretical mass of the deprotonated molecule (M − H⁺, 503.0510), with an additional signal at 549.0565 corresponding to the formic acid (used for the mass analysis) adduct (M−H+/HCOOH, theoretical mass 549.0565). Both signals displayed the typical pattern (⁷⁶Se, 9.37%, ⁷⁷Se, 7.63%, ⁷⁸Se, 23.77%, ⁸⁰Se, 49.61%, ⁸²Se, 8.73%) of molecules containing one selenium atom. Heteronuclear Single Quantum Correlation (HSQC) spectroscopy was used to assign ¹³C signals to compound 3, as shown in

Table 1. ¹H and ¹³C chemical shifts (ppm) for compound 3.

Position	δH	δC
2	---	161.40
3	6.99	126.06
4	---	143.05
5	---	103.00
6	7.01	141.28
7	9.86	191.78
8	5.01	52.31
9	---	134.48
10	7.15	128.81
11	7.36	129.32
12	7.31	128.95

.

However, an ambiguity concerning the position of selenium on the heterocycle remained. On the ¹H-NMR spectrum, the absence of coupling between the two protons of the heterocycle, which appear as singlets at 6.99 and 7.01 ppm, shows that selenium is not in position 3 on the ring. A correlation observed in the Nuclear Overhauser Effect spectroscopy (NOESY) spectrum between a heterocyclic proton (i.e., H6 proton) and the benzylic protons (H8) led to the conclusion that selenium should be located at position 5 (Figure 2).

3. Materials and Methods

All reagents and solvents were purchased from commercial sources (Sigma Aldrich or Alfa Aesar) and were used without further purification. ¹H-NMR and ¹³C-NMR spectra were recorded on Bruker Advance 300 and 500 spectrometers, respectively, and the residual proton signals of deuterated CDCl₃ were used as an internal reference (δ = 7.26 ppm and 77.00 ppm, respectively). Proton coupling patterns are abbreviated as “s” for singlet, “dd” for doublet of doublets, and “m” for multiplet. The high-resolution mass spectrum (HRMS) was recorded on a Xevo G2 QTOF (Waters, Milford, CT, USA).

Synthesis of Compounds 2 and 3

Selenium oxide (0.3 mmol, 33.3 mg, 1.2 equiv.) under argon followed by anhydrous 1,4-dioxane (0.1 mL) were added to a 25 mL round-bottomed flask containing 1-benzyl-4-methylpyridin-2(1H)-one (0.25 mmol, 50 mg, 1 equiv.). The mixture was warmed up to reflux for 20 h, and then cooled down at room temperature. Ethyl acetate was added, the resulting mixture was dried over magnesium sulfate, and the solvent was concentrated under vacuum. The purification of the products was performed by using a 12 g prepacked column on a flash chromatography (25 mL/min, detector at 254 nm, 2 min at 40–60 petroleum ether–ethyl acetate, and then gradient from 40 to 60 to 10–90 petroleum ether–ethyl acetate in 20 min). Melting point (Mp) values were measured in open capillary tubes on a Metler Toledo MP50 melting point system and are uncorrected.

1-Benzyl-2-oxo-1,2-dihydropyridine-4-carbaldehyde (2) Light-yellow sticky solid (31%, 17 mg). ¹H-NMR (300 MHz, DMSO-D₆): δ ppm 9.87 (s, 1H); 7.40–7.28 (m, 6H); 7.04 (dd, J = 1.8 Hz, 0.5 Hz, 1H); 6.55 (dd, J = 7.0 Hz, 1.9 Hz, 1H); 5.16 (s, 2H); ¹³C-NMR (75 MHz, DMSO-D₆): δ ppm 191.12; 162.64; 144.87; 138.55; 135.56; 129.03; 128.35; 128.21; 126.63; 101.05; 52.27; HRMS calculated for C₁₃H₁₂NO₂ (ESI, M + H⁺): 214.0868. Found: 214.0873.

5,5’-Selenobis(1-benzyl-2-oxo-1,2-dihydropyridine-4-carbaldehyde) (3) Orange powder (18.5 mg, 29%). Mp = 81.5–82.5 °C. ¹H-NMR (300 MHz, DMSO-D₆): δ ppm 9.86 (s, 1H); 7.36 (m, 1H); 7.31 (m, 2H); 7.15 (m, 2H); 7.01 (s, 1H); 6.99 (s, 1H); 5.01 (s, 2H); ¹³C-NMR (150 MHz, DMSO-D₆): δ ppm 191.78; 161.40; 143.05; 141.28; 134.48; 129.32; 128.95; 128.81; 126.06; 103.00; 52.31; HRMS calculated for C₂₆H₁₉N₂O₄Se (ESI, M − H⁺ and M + HCOO⁻): 503.0510 and 549.0565. Found: 503.0524 and 549.0565.