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Article

A New Species of Lycodapus from the Emperor Seamount Chain, Northwestern Pacific Ocean (Teleostei: Zoarcidae) †

by
Artem M. Prokofiev
1,2,
Andrei A. Balanov
3,
Olga R. Emelianova
4,
Alexei M. Orlov
2,5,6,7,8,* and
Svetlana Yu. Orlova
4,9
1
Laboratory of Ecology of Aquatic Communities and Invasions, A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninsky Prospekt 33, 119071 Moscow, Russia
2
Laboratory of Oceanic Ichthyofauna, P.P. Shirshov Institute of Oceanology of the Russian Academy of Sciences, 117218 Moscow, Russia
3
Laboratory of Ichthyology, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia
4
Laboratory of Molecular Genetics, Russian Federal Research Institute of Fisheries and Oceanography, 107140 Moscow, Russia
5
Department of Ichthyology and Hydrobiology, Tomsk State University, 634050 Tomsk, Russia
6
Department of Ichthyology, Dagestan State University, 367000 Makhachkala, Russia
7
Laboratory of Marine Biology, Caspian Institute of Biological Resources of Dagestan Federal Research Center of the Russian Academy of Sciences, 367000 Makhachkala, Russia
8
Laboratory of Behavior of Lower Vertebrates, A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, 119071 Moscow, Russia
9
Laboratory of Ecology of Coastal Bottom Communities, P.P. Shirshov Institute of Oceanology of the Russian Academy of Sciences, 117218 Moscow, Russia
*
Author to whom correspondence should be addressed.
Publications: urn:lsid:zoobank.org:pub:FA3A9FAF-45E1-4553-8BF2-E7E6D22F7328.
Diversity 2022, 14(11), 972; https://doi.org/10.3390/d14110972
Submission received: 14 October 2022 / Revised: 9 November 2022 / Accepted: 10 November 2022 / Published: 11 November 2022

Abstract

:
A new species, Lycodapus imperatorius, is described from the seamounts of the Emperor Ridge, North Pacific Ocean. The new species can be identified by its stout gill rakers, single interorbital pore, four preopercular and four mandibular pores, 95–99 vertebrae, numerous vomerine and palatine teeth, and COI mtDNA sequences. Although the new species is most similar to L. endemoscotus and L. antarcticus in morphology, the closest match to already published sequences was Lycodapus fierasfer, which is fairly different from the new species in terms of morphology. The incongruence between molecular and morphological inferences might be explained by the homoplastic nature of the morphological characteristics used for species delimitation in Lycodapus. The percent of genetic identity between the closest species of Lycodapus ranges from 95.4 to 98.6% in comparison with 99.5–100.0% between individuals of the same valid species. A key of the genus is amended to include the new species.

1. Introduction

The zoarcid genus Lycodapus Gilbert, 1891 was reviewed in [1,2], where there were twelve valid and one unnamed species defined; the latter was subsequently described as L. antarcticus [3]. This genus is very peculiar in the family due to its compressed head and body with loose naked skin, terminal mouth with oblique mouth gape, large gill opening, and head pore pattern [1]. Eleven of the thirteen species are known to be from the North Pacific Ocean, ranging from the Bering Sea in the south to the Sea of Okhotsk in the western North Pacific and to Panama in the eastern Pacific [4]. A single specimen of this genus was reported from the Emperor Seamounts as Lycodapus sp. [5], which was later reiterated in [6,7]. In April 2019, during a research cruise onboard R/V “Professor Kaganovsky” [8], the second specimen of an unidentified Lycodapus species was found in a catch off the Emperor Seamount [9], where two other new deep-water species were recently discovered [10,11]. A thorough examination of these two specimens reveals their attribution to a new species, the description of which is an aim of the present paper.

2. Materials and Methods

The type specimens of the new species are housed in the Institute of Oceanology, Russian Academy of Sciences, Moscow (IORAS) and in the Museum of the A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences (MIMB). Catalog numbers and label data are presented under species description. The new species has been registered in ZooBank (urn:lsid:zoobank.org:act:321864B9-B477-48C1-8AAB-E3195562FF78).
Definitions of the morphological characteristics and measurements follow [1,12]. Rays in unpaired fins and vertebrae were counted from radiographs. In the description, the characteristics or values for the paratype, when different from the holotype, are enclosed in brackets. Counts that differed on opposite sides of the same specimen are separated by slash (/). Abbreviations used: R/V, research vessel; TL and SL, total and standard length, respectively.
The tissue samples used for genetic analysis are listed in Table 1. All tissue samples were fixed in volumes of 96% ethanol at least five times larger than the sample volume. Fixed samples were stored at −20 °C; ethanol was changed approximately one month after collection and again after one year. DNA was extracted using the Wizard SV 96 Genomic DNA Purification System (Promega Corporation, Madison, WI, USA) according to the manufacturer’s manual. All molecular genetic studies (DNA extraction, polymerase chain reaction (PCR), PCR product purification, and nucleotide sequencing) were performed using standard molecular genetic techniques [13]. Cytochrome oxidase subunit I (COI) fragment was amplified with a primer complex of VF2_t1, FishF2_t1, FishR2_t1, FR1d_t1 [13,14]. Amplification was conducted in a volume of 15 μL with 90 ng total DNA, buffer x1, 2.5 mM MgCl2, 0.2 mM dNTP, 0.5 mM of each primer, and 0.75 U μL−1 Color Taq polymerase. Cycling consisted of 5 min at 95 °C, followed by thirty-five cycles of 30 s each at 95 °C, 45 s at 52 °C, 60 s at 72 °C, and a final extension for 12 min at 72 °C. All resulting amplicons were purified by ethanol precipitation [15].
Purified fragments were sequenced from both strands by Applied Biosystems BigDye Terminator v3.1. kit (Applied Biosystems, Foster City, CA, USA) with capillary electrophoresis on an ABI3500 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA) in the VNIRO Laboratory of Molecular Genetics.
The resulting sequences were assembled in Geneious 6.5.0 (Biomatters, Auckland, New Zealand) [16] and aligned with the “ClustalW” built-in algorithm. The “postfix” was set to a maximum desired length after trimming of 565 bases, was trimmed more from the 3-end if necessary, and removed any leading and trailing ambiguous bases. Any sequences whose lengths were less than 565 bp were considered failures and were removed from the analysis. Next, the bidirectional sequences were assembled into contigs (with default settings: using dirty data algorithm, realigner, and prefer 3gap placement, as well as a 20-base minimum overlap and an 85% minimum match percentage). At this point, if any contig contained >2% ambiguities, those samples were also removed. Any remaining single-read sequences were used if their quality value was better than 98%.
The resulting sequences were subsequently translated into the necessary format to construct a haplotype network in the PopArt program (Allan Wilson Centre Imaging Evolution Initiative, Otago, New Zealand) [17]. The FaBox 1.41 converter was used to convert the fasta file to the format required for calculation [18]. A network of haplotypes was constructed based on the maximum parsimony method using TCS v.1.21 software (Computational Science Laboratory, Provo, UT, USA). DnaSP v. 5.10.01 software (University of Barcelona, Barcelona, Spain) was used for the analysis of the average number of nucleotide substitutions and the number of haplotypes in samples [19].
Data processing was performed, and genetic distances and percent identity were calculated using Geneious 6.0.5 software (Biomatters, Auckland, New Zealand) based on the Bayesian Inference method with Bathymaster signatus as the outgroup [5,6,7,20,21] with the use of a Substitution Model HKY85; 1,100,000 chain length; and 100,000 burn-in-length [22].
Data on the COI sequences of the outgroup and congeners of a new species (sister groups involved in an analysis for comparative purposes) were taken from the open NCBI (https://www.ncbi.nlm.nih.gov/, accessed on 3 November 2022) and BOLD Systems (https://www.boldsystems.org/, accessed on 3 November 2022) databases.

3. Results

Lycodapus imperatorius sp. nov.
urn:lsid:zoobank.org:act:321864B9-B477-48C1-8AAB-E3195562FF78
Holotype: IORAS 03641, female, TL 144 mm, SL 140 mm, R/V “Professor Kaganovsky”, 11.04.2019, bottom trawl, haul 107, Lira Seamount, 36°48′08′′–36°47′09′′ N, 171°23′00′′–171°23′05′′ E, depth 643–649 m, DNA-sample 89, collector A.M. Orlov.
Paratype: MIMB 43527, female, TL 132 mm, SL 128 mm, R/V “TINRO”, 06.04.2010, bottom trawl, haul 75, Jingu Seamount, 38°43′01′′ N, 171°07′05′′ E, depth 806–822 m, collector A.A. Balanov.
Diagnosis. Identified by the stout gill rakers (gill raker ratio 58–66%), single interorbital pore, four preopercular and four mandibular pores, 95 to 99 vertebrae, 16 vomerine and 24–29 palatine teeth in females, upper rim of eye below dorsal contour of head, upper end of gill slit level to just above upper end of pectoral-fin base, and snout relatively long (32.1–35.2% of head length).
Description. Measurements shown in Table 2. Vertebrae 99 (17 + 82) [95 (16 + 79)]. Body elongate, tapering posteriorly, compressed laterally, slightly humped at nape. Dorsal and anal fins continuous, confluent with small truncate caudal fin. Dorsal-, anal-, and caudal-fin rays 101 [89], 83 [80], and 10 (2 + 4 + 4) in number, respectively. Dorsal-fin origin situated slightly closer to the base than to the tip of pectoral fin adpressed to body. First dorsal-fin pterygiophore inserted between fourth and fifth [fifth and sixth] neural spines. Longest dorsal-fin rays about as long as pectoral rays; longest anal-fin rays about 1.5 times shorter than those of dorsal fin. Pectoral fin small, narrow-based, rounded at tip, 3.3 [2.8] times in head. Pectoral-fin rays 9 [8], covered with loose transparent skin. Pelvic fins absent. Head moderate, 5.3 [5.4] times in SL, with dorsal contour concave before eyes. Eye lateral in position, not entering dorsal profile of head, 6.2 [5.2] times in head length. Interorbital space convex, slightly (1.2 [1.1] times) exceeding eye diameter in width. Nostril with slightly raised rim [tubular]. Mouth gape moderately large, oblique; upper jaw reaching to mid-eye. Symphyseal knob of lower jaw small, aciculate; mandibular joint forming a conspicuous triangular protrusion. Gill slit originating at level of upper end of pectoral-fin base or just above it, extending forward to vertical of posterior third of eye. Gill rakers short and broad-based (length 1.5 times greater than width at base), with tips truncated, armed with small denticles (10–12 denticles per raker), 12 (2 + 10) [14–15 (2 + 12/2 + 13)] on first arch; relative gill raker ratio 58 [66]. Two pseudobranchial filaments.
Jaw dentition villiform, arranged in bands, teeth in outermost row near the symphyses slightly enlarged and curved outward, more conspicuously on premaxilla. Vomerine and palatine teeth uniserial, pointed; those on vomer slightly longer, somewhat greater than the outermost curved teeth on premaxilla, arranged in broadly V-shaped series and slightly increasing in length posteriad (but hindmost tooth on both sides small). Palatine teeth nearly uniform in size. There are 16 vomerine and about 27/29 [24/26] palatine teeth.
Pores in cephalic laterosensory system: supraorbital, 3 (nasal, preorbital, and postorbital pores); interorbital (coronal), 1; mandibular, 4; preopercular, 4. Infraorbital canal and pores absent. Free lateralis organs on side of body inconspicuous, discernible only in mediolateral line above gill opening and pectoral-fin base.
Skin naked, thin, movable. Anus close to anal-fin origin. Pyloric caeca absent.
Preserved specimens pale-colored with skin semitransparent, whole head and body densely peppered by black dotted melanophores on a diffuse brownish subcutaneous pigmentation becoming more pronounced caudally; dorsal and anal fins hyaline with rays peppered with small black melanophores becoming much denser caudally, where melanophores confluent to almost uniform blackish pigmentation along hindmost portions of dorsal and anal fins. Pectoral fin unpigmented. Branchiostegal membrane, orobranchial cavity and dorsal surface of tongue dotted with small blackish melanophores [uniformly dark in paratype]; gill arches and pseudobranchial filaments unpigmented. Peritoneum brownish to blackish, not solidly pigmented; stomach with longitudinal streaks of melanophores dorsally; intestine unpigmented. In fresh specimen, body was more brownish, darkened toward tail tip, and abdomen was bluish with partly translucent blackish peritoneum.
Etymology. The species name is given in reference to the type of locality of the species (Emperor Seamount Chain).
Genetic analysis. Molecular data indicate that the new species Lycodapus imperatorius sp. nov. seems genetically closer to L. fierasfer from the northeastern Pacific Ocean than to L. endemoscotus from the same region, to which it is more morphologically similar (Figure 2). Moreover, L. imperatorius and L. fierasfer form a separate clade on the phylogenetic tree, which is well-separated from the clade formed by L. mandibularis (northeastern Pacific Ocean), L. endemoscotus (northeastern Pacific Ocean), L. antarcticus (Southern Ocean), and the undescribed species Lycodapus sp. (records from Costa Rica to the Southern Ocean). The clade represented by L. pachysoma from the waters of British Columbia and the Southern Ocean is well-separated from these two clades.
Phylogenetic relationships (Figure 3) testify that the new species is genetically closest to L. fierasfer, with which it has 97.4–97.8% genetic identity. A little more (97.3–97.6% identity) L. imperatorius is separated from L. antarcticus. It should be noted that the percent of identity between closest species of the genus Lycodapus ranges from 95.4 to 98.6%, while that between individuals of the same valid species amounts to 99.5–100.0%. The maximum % identity (98.5–98.6%) was observed between L. antarcticus and L. endemoscotus, as well as between L. antarcticus and the undescribed L. sp. (97.4–98.6%). The minimum % identity (96.0–96.6%) was recorded between L. mandibularis and L. pachysoma.

4. Discussion

When comparing the new species with its congeners in external features, Lycodapus imperatorius is easily confused with L. endemoscotus from the eastern North Pacific between 27 and 50° N [1]. The two species are almost identical in morphology except for the higher vertebral count in the new species (95–99 vs. 87–95 in L. endemoscotus) and relative position of the upper ends of gill slit and pectoral-fin base (these are more closely spaced in the new species, with the distance between them equal to 0–5.0% of the head length instead of 4.2–12.1% in L. endemoscotus). However, the percent of identity between L. imperatorius and L. endemoscotus is 96.9–97.1%, and these species fall into different clades on the phylogenetic tree (Figure 2 and Figure 3).
Although L. imperatorius and L. fierasfer are the closest genetic relatives, these species are strikingly different from each other in morphology. The new species differs from L. fierasfer in the stout (vs. long) gill rakers (gill raker ratio 58–66% vs. 117–256% (usually 150% or more) in L. fierasfer), four (vs. three) mandibular and preopercular pores, single (vs. typically two) interorbital pore, and higher total and precaudal vertebral count (95–99 and 16 or 17 vs. 83–91 and 13–15). Such incongruence between the molecular and morphological inferences might be explained by the homoplastic nature of the morphological characteristics used for the species delimitation in Lycodapus.
The new species is also similar, both morphologically and genetically, to L. antarcticus known circumglobally from the Southern Ocean. However, the new species can be separated from L. antarcticus by the longer snout (32.1–35.2% vs. 24.7–31.5% of head length), more numerous palatine teeth (24–29 vs. 6–23 in L. antarcticus), less numerous vertebrae (95–99 vs. 98–105 in L. antarcticus) [3,23], and 97.3–97.6% genetic identity. The new species differs from L. leptus from the Bering Sea by the presence of four normally developed preopercular pores (vs. three pores, often secondarily closed in adults), numerous palatine teeth (24–29 vs. 0–5), and a longer head (19% vs. 13–15% SL) [2]. The new species can be easily distinguished from the other short and stout gill-raker species (L. mandibularis, L. microchir and L. pachysoma) by its higher vertebral count (95–99 vs. 81–96, increasing in more northern populations of L. mandibularis; 75–85 in L. microchir and L. pachysoma) and further differs from L. mandibularis by the more numerous vomerine and palatine teeth (16 and 24–29 vs. 0–5 and 3–13, respectively) and by the eye not entering the dorsal profile of the head (vs. eye interrupts the concave dorsal profile in L. mandibularis) [2].

5. Key to Species of Lycodapus

(Modified from [2])
  • 1A. Gill rakers on first gill arch very short and blunt; gill raker ratio less than 30% ……………………………………………………………………………… 2
  • 1B. Gill rakers stout to long; gill raker ratio more than 30% …………………………………………………………………………………………………………. 3
  • 2A. Four preopercular pores; vomerine plus inner row of dentary teeth enlarged and not sexually dimorphic in adults; gill slit not extending above pectoral base (Strait of Juan de Fuca to Aleutian Islands) ………………………………………………………………………………………………………………………………………Lycodapus parviceps
  • 2B. Three preopercular pores; vomerine and dentary teeth small; gill slit extending slightly above pectoral fin base (Bering Sea) ………………………………………………………………………………………………………………………………………………………Lycodapus psarostomatus
  • 3A. Gill rakers of first gill arch, blunt and stout (pointed in young L. mandibularis), gill raker ratio usually 30 to 150% (rarely 170% in juveniles); when rakers of first arch are pressed downward against the arch, their tips usually lie close to and not beyond base of the adjacent raker down the arch; one median interorbital pore, four mandibular pores, four preopercular pores (except L. leptus has less than four preopercular pores) ………………………………………………………………………………………………………..4
  • 3B. Gill rakers of first gill arch, long, slender and pointed; gill raker ratio usually between 150 and 270%, if less than 150% the gill rakers are pointed and there are three preopercular pores; when rakers pressed downward against the arch, their tips usually lie closer to base of second raker down the arch than base of adjacent raker; a median or paired interorbital pore; three or four mandibular pores ……………………………………………………………………………………………………………………………………………………10
  • 4A. Total number of vertebrae 75 to 82 (Antarctic and North Pacific Oceans) …………………………………………………………………………………………………………………………………………………………..Lycodapus pachysoma
  • 4B. Total number of vertebrae more than 82 ………………………… ………………………………………………………………………………………………..5
  • 5A. Total number of vertebrae 98 to 105; four preopercular pores, snout length less than 32% of head length (Antarctic waters) ……………………… …………………………………………………………………………………………………………………………………………………………Lycodapus antarcticus
  • 5B. Total number of vertebrae 99 or less; if more than 96, then three preopercular pores or snout length greater than 32% of head length …………………………….………….………… ………………………………………………………………………………………………………………………………………………………………………………..6
  • 6A. Three preopercular pores, often secondarily closed in adults; total number of vertebrae, 94 to 99; palatine teeth, 0–5; head length 13 to 15% of SL (Bering Sea) ……………………………………………………………………………………………………………………………………………………………….Lycodapus leptus
  • 6B. Four preopercular pores; total number of vertebrae, 79 to 99 (if more than 96 then palatine teeth numerous); head length 12 to 23% (usually more than 15%) of SL ………………………………………………………………………………………………………………………………………………………………………………7
  • 7A. Vomerine teeth 0 to 5; palatine teeth 3 to 13 (usually less than 10 on each side); upper rim of eye reaches level of dorsal profile of head; gill raker ratio about 65 to 165% (lower in adults) (total number of vertebrae 81 to 96; usually inhabits midwater at depths less than 700 m off western North America) …………Lycodapus mandibularis
  • 7B. Vomerine teeth 16 to 21 (reduced to 4 on some mature males); palatine teeth 8 to 29 on each side; upper rim of eye of adults below dorsal profile of head; gill raker ratio 49 to 106% ………………………………………………………………………………………………………………………………………………………………………………8
  • 8A. Total number of vertebrae 79 to 85 (Sea of Okhotsk and probably Bering Sea) …………………………….…………………………Lycodapus microchir
  • 8B. Total number of vertebrae 87 to 99 …………………………………………………………………………………………………………………………………9
  • 9A. Total number of vertebrae 95 to 99; upper end of gill slit level to upper end of pectoral-fin base or only slightly above it; known from near-bottom layer at depths 643–822 m over the Emperor Seamounts ……………………………………………………………………………………………………………………Lycodapus imperatorius sp. nov.
  • 9B. Total number of vertebrae 87 to 95; upper end of gill slit well above from upper end of pectoral-fin base; inhabits depths of 933 to 2225 m off western North America, south of 50° N ……………………….………………….………………………………………………………………………………………………………..Lycodapus endemoscotus
  • 10A. Vomerine teeth absent; total number of vertebrae 73 to 84; precaudal vertebrae 14 or 15; a median interorbital pore; three preopercular pores (Sea of Okhotsk and western Bering Sea) ………………………………………………………….……………………………………………………………………………………………..Lycodapus derjugini
  • 10B. Vomerine teeth present; total number of vertebrae 77 or more; precaudal vertebrae 13 to 18; three or four preopercular pores ……………………………………………………………………………………………………………………………………………………………………………..11
  • 11A. Usually two interorbital pores; three preopercular pores (sometime four in L. australis); three mandibular pores (sometimes four in L. australis); vertebrae 83 or more (if four preopercular or mandibular pores then 3 to 14 palatine teeth) …………….…….………………………………………………………………………………..12
  • 11B. One interorbital pore; three preopercular pores (frequently four in L. dermatinus); four mandibular pores (frequently three in L. dermatinus); total number of vertebrae 76 to 87 ……………………………………………………………………………………………………………………………………………………………………………..13
  • 12A. Lips with dark pigment; precaudal vertebrae 13 to 15; inhabits northeastern Pacific Ocean and Bering Sea) …………………………………………………………………………………………………………………………………………………………..Lycodapus fierasfer
  • 12B. Lips without dark pigment; precaudal vertebrae 15 to 18; inhabits areas around Straits of Magellan) ………………………………………………………………………………………………………………………………………………………….Lycodapus australis
  • 13A. Palatine teeth 2 to 26 (usually 6 to 18); vomerine teeth 3 to 19 (usually 5 to 12); total number of vertebrae 76 to 82; precaudal vertebrae 13 to 15 (northeast Pacific south of 56° N latitude) ……………………………………………………………………… ………………………………………………………………………………Lycodapus dermatinus
  • 13B. Palatine teeth 0–4 (usually 0–2); vomerine teeth 1–6 (usually 2–5); total number of vertebrae 80–87; precaudal vertebrae 15–17 (Bering Sea) …………………………………………………………………………………………………………………………………………………………… Lycodapus poecilus

6. Research Gaps and Future Directions

In conclusion, we outline some research gaps and possible directions for future taxonomic research of the studied species of the subfamily Lycodinae based on the conducted genetic analysis. Firstly, the presence of an undescribed Lycodapus species among the studied specimens is noteworthy, which is genetically closest to L. endemoscotus and L. antarcticus, with records in the waters of Costa Rica, Panama, and the Southern Ocean. The next step should be its morphological study, followed by a taxonomic description.
Genetic analysis revealed significant intraspecific variations in some Lycodapus species. For example, L. pachysoma has two clades on the phylogenetic tree, represented by specimens from the Southern Ocean (Nos. 18 and 19) and the waters of British Columbia (Nos. 20–27). Given such a disjunct distribution, it is possible to conclude with a high degree of probability that, in this case, we are dealing with two distinct species. At the same time, genetic analysis showed that there are also significant differences between specimens from the waters of British Columbia (Nos. 25 and 22 vs. the rest ones), which may indicate the presence of a cryptic species in this area. All of the above requires additional taxonomic studies.
Another interesting aspect is the high species diversity of the genus Lycodapus in the northeastern Pacific, which requires appropriate research aimed at identifying the center of speciation and ways of further dispersion of representatives of this genus.
So far, the records of the Lycodapus species in the northwestern Pacific are very rare, and they are limited to the waters of the southern Sea of Okhotsk and Eastern Kamchatka [4,24]. Since the taxonomic status of the captured specimens remains unclear, further morphological and genetic study of them seems quite relevant.
Among the genetically studied representatives of the genus Bothrocara, B. hollandi stands out, previously referred to the genus Allolepis. Genetically, this species is closer to Bothrocarina microcephala and Lycodapus mandibularis than to its congeners (95.4–95.7% vs. 93.3–95.0% identity, respectively). These results require additional studies aimed at revising the taxonomic status of B. hollandi.

Author Contributions

Conceptualization, A.M.P., A.A.B. and A.M.O.; methodology, A.M.P., A.A.B., O.R.E. and S.Y.O.; software, O.R.E., A.M.O. and S.Y.O.; validation, A.M.P., A.A.B., O.R.E., A.M.O. and S.Y.O.; formal analysis, A.M.P., A.A.B., O.R.E. and S.Y.O.; investigation, A.M.P., A.A.B., O.R.E., A.M.O. and S.Y.O.; resources, A.M.P., A.A.B., O.R.E., A.M.O. and S.Y.O.; data curation, A.M.P., A.A.B., O.R.E., A.M.O. and S.Y.O.; writing—original draft preparation, A.M.P. and A.M.O.; writing—review and editing, A.M.P., A.A.B., A.M.O. and S.Y.O.; supervision, A.M.O. All authors have read and agreed to the published version of the manuscript.

Funding

Preparation of this paper was funded by the Ministry of Science and Higher Education, Russian Federation (grant No. 13.1902.21.0012, contract No. 075-15-2020-796).

Institutional Review Board Statement

Not applicable.

Data Availability Statement

The specimens described in this study are available at Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow (IO RAS) and at the Museum of the A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences (MIMB). Voucher IDs: Lycodapus imperatorius sp. nov.: holotype IORAS 03641, paratype MIMB 43527. The COI sequence that supports the findings of this study has been deposited in NCBI GenBank with the ascension code (OP759467) for Lycodapus imperatorius. The new species registration of Lycodapus imperatorius in Zoobank with LSID: urn:lsid:zoobank.org:act:321864B9-B477-48C1-8AAB-E3195562FF78. The publication LSID: urn:lsid:zoobank.org:pub:FA3A9FAF-45E1-4553-8BF2-E7E6D22F7328.

Acknowledgments

The authors are grateful to their colleagues from TINRO (Pacific Branch of VNIRO, Vladivostok, Russia) who took part in the processing of catches from the cruise of the R/V “Professor Kaganovsky” in 2019, storing samples in TINRO, and transporting them to Moscow. They also thank Olga Radchenko (Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences, Magadan, Russia) for consultations regarding the genetic analysis of the paratype specimen of Lycodapus imperatorius sp. nov. The authors acknowledge the assistance of their colleagues James Orr and Katherine Pearson Maslenikov (University of Washington Fish Collection, School of Aquatic and Fishery Sciences and Burke Museum of Natural History and Culture, Seattle, WA, USA) and Gavin Hanke (Royal British Columbia Museum, Victoria, B.C., Canada) who provided images of L. endemoscotus used for comparative purposes. Special thanks to Kirill Kolonchin (VNIRO) and Aleksey Baitaliuk (TINRO), who approved the participation of A.M.O. and S.Y.O. in the cruise. The authors also thank the four anonymous reviewers for their valuable comments and suggestions that allowed for improvement of this manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

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Figure 1. Lycodapus imperatorius sp. nov., holotype, IORAS 03641, 140 mm SL: (A) fresh caught, (B) after thawing.
Figure 1. Lycodapus imperatorius sp. nov., holotype, IORAS 03641, 140 mm SL: (A) fresh caught, (B) after thawing.
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Figure 2. Tree topology reconstruction of specimens of Lycodapus imperatorius sp. nov, its congeners, sister group, and outgroup based on the Bayesian Inference Substitution Model HKY85; 1,100,000 chain length; and 100,000 burn-in-length for mitochondrial cytochrome oxidase subunit I (COI) genes. Numbers beside each branch indicate bootstrap values. Numbers to the right of the branches correspond to the number of specimens in Table 1.
Figure 2. Tree topology reconstruction of specimens of Lycodapus imperatorius sp. nov, its congeners, sister group, and outgroup based on the Bayesian Inference Substitution Model HKY85; 1,100,000 chain length; and 100,000 burn-in-length for mitochondrial cytochrome oxidase subunit I (COI) genes. Numbers beside each branch indicate bootstrap values. Numbers to the right of the branches correspond to the number of specimens in Table 1.
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Figure 3. Phylogenetic relationships of Lycodapus imperatorius sp. nov. and allied species by COI mtDNA sequences. Numbers indicate haplotypes (see Table 1 for explanation), numbers of substitutions are given in brackets, size of black circles correspond to number of specimens.
Figure 3. Phylogenetic relationships of Lycodapus imperatorius sp. nov. and allied species by COI mtDNA sequences. Numbers indicate haplotypes (see Table 1 for explanation), numbers of substitutions are given in brackets, size of black circles correspond to number of specimens.
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Table 1. Information about COI sequences and respective samples used for molecular analysis (NA—not available).
Table 1. Information about COI sequences and respective samples used for molecular analysis (NA—not available).
GroupSpecimen NumberSpeciesCatalog ID of Voucher SpecimensGenBank Ascension NumberCapture AreaVoucher Specimen Depository/Source of COI Sequences
New species1Lycodapus imperatorius sp. nov.IORAS 03641OP759467Emperor SeamountsInstitute of Oceanology, Moscow, Russia/NCBI
28Lycodapus imperatorius sp. nov.MIMB 43527ANGBF4848-12Emperor SeamountsInstitute of Marine Biology, Vladivostok, Russia/[5,6,7], NCBI
Congeners2Lycodapus antarcticusSC049627FOAG506-08Herd and McDonalds IslandsAustralian Antarctic Division, Hobart, Australia/BOLD Systems
3Lycodapus antarcticusSC049620FOAG507-08Herd and McDonalds IslandsAustralian Antarctic Division, Hobart, Australia/BOLD Systems
4Lycodapus antarcticusSC109846FOAG558-08Southern Ocean, Plateau NorthCSIRO, Australian National Fish Collection, Hobart, Australia/BOLD Systems
6Lycodapus endemoscotusNATZFPA064-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
7Lycodapus endemoscotusNATZFPA065-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
8Lycodapus fierasferNAANGBF4849-12No dataNA/Mined from GenBank, NCBI
9Lycodapus fierasferUW113553FMV276-08Oregon, USAUniversity of Washington, Fish Collection, Seattle, USA/BOLD Systems
10Lycodapus fierasferUW113567FMV284-08Oregon, USAUniversity of Washington, Fish Collection, Seattle, USA/BOLD Systems
11Lycodapus fierasferSIO 06-27MFC401-08Oregon, USAScripps Institution of Oceanography, San Diego, USA/BOLD Systems
12Lycodapus fierasferRBCM-006-028-018TZFPB306-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
13Lycodapus fierasferRBCM-006-028-019TZFPB307-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
15Lycodapus mandibularisNATZFPA199-07British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
16Lycodapus mandibularisNATZFPA201-07British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
18Lycodapus pachysoma2009-1380EATF164-10Southern OceanMuseum National d’Histoire Naturelle, Paris, France/BOLD Systems
19Lycodapus pachysoma2009-0026EATF403-10Southern OceanMuseum National d’Histoire Naturelle, Paris, France/BOLD Systems
20Lycodapus pachysomaNATZFPA017-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
21Lycodapus pachysomaNATZFPA018-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
22Lycodapus pachysomaNATZFPA019-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
23Lycodapus pachysomaNATZFPA020-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada /BOLD Systems
24Lycodapus pachysomaNATZFPA021-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
25Lycodapus pachysomaNATZFPA066-06British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
26Lycodapus pachysomaRBCM-006-034-021TZFPB356-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
27Lycodapus pachysomaRBCM-006-034-022TZFPB357-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
29Lycodapus sp.UW 150605FMV581-11Northeastern Pacific, USAUniversity of Washington, Fish Collection, Seattle, USA/BOLD Systems
30Lycodapus sp.NAGBMND68044-21Southern OceanNA/Mined from GenBank, NCBI
31Lycodapus sp.NAGBMND68045-21Southern OceanNA/Mined from GenBank, NCBI
32Lycodapus sp.USNM 422332MOP075-12Pacific Ocean, PanamaSmithsonian Institution, National Museum of Natural History, Washington, USA/BOLD Systems
33Lycodapus sp.USNM 421188MOP643-12Pacific Ocean, Costa RicaSmithsonian Institution, National Museum of Natural History, Washington, USA/BOLD Systems
Sister group34Bothrocara brunneumKU 28306UKFBI299-08California, USAUniversity of Kansas, Biodiversity Research Center, Lawrence, USA/BOLD Systems
35Bothrocara brunneumRBCM-006-030-006TZFPB324-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
36Bothrocara brunneumSIO 05-87MFC324-08California, USAScripps Institution of Oceanography, San Diego, USA/BOLD Systems
37Bothrocara brunneumUW 119872FMV471-11Washington, USAUniversity of Washington, Fish Collection, Seattle, USA/BOLD Systems
38Bothrocara hollandiNAGBGC6548-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
39Bothrocara hollandiKC748099GBGCA4726-13Sea of JapanDepartment of Marine Biology in Pukyong National University, Busan, Republic of Korea/Mined from GenBank, NCBI
40Bothrocara hollandiNAANGBF56896-19South KoreaNA/Mined from GenBank, NCBI
41Bothrocara hollandiNAANGBF56898-19South KoreaNA/Mined from GenBank, NCBI
42Bothrocara hollandiNAANGBF56909-19South KoreaNA/Mined from GenBank, NCBI
43Bothrocara molleSIO 05-165MFC333-08California, USAScripps Institution of Oceanography, San Diego, USA/BOLD Systems
44Bothrocara molleUSNM 422430MOP063-12Pacific Ocean, PanamaSmithsonian Institution, National Museum of Natural History, Washington, USA/BOLD Systems
45Bothrocara molleRBCM-006-034-003TZFPB338-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
46Bothrocara molleRBCM-006-034-002TZFPB337-05British ColumbiaRoyal British Columbia Museum, Victoria, Canada/BOLD Systems
47Bothrocara molleUSNM 423188MOP198-12Pacific Ocean, Costa RicaSmithsonian Institution, National Museum of Natural History, Washington, USA/BOLD Systems
48Bothrocara zestumMIMB 22383FERU090-13Western Bering SeaInstitute of Marine Biology, Vladivostok, Russia/ BOLD Systems
49Bothrocara zestumMIMB 22384FERU094-13Western Bering SeaInstitute of Marine Biology, Vladivostok, Russia/ BOLD Systems
50Bothrocara zestumMIMB 22386FERU092-13Western Bering SeaInstitute of Marine Biology, Vladivostok, Russia/BOLD Systems
51Bothrocara zestumMIMB 22384GBGCA3018-13Western Bering SeaNA/Mined from GenBank, NCBI
52Bothrocarina microcephalaNAGBGC6192-08Northern Sea of OkhotskNA/Mined from GenBank, NCBI
53Bothrocarina microcephalaNAGBGC6193-08Northern Sea of OkhotskNA/Mined from GenBank, NCBI
54Bothrocarina nigrocaudataNAGBGC6194-08Northern Sea of OkhotskNA/Mined from GenBank, NCBI
55Bothrocarina nigrocaudataNAGBGC6195-08Northern Sea of OkhotskNA/Mined from GenBank, NCBI
56Bothrocarina nigrocaudataNAGBGC6196-08Northern Sea of OkhotskNA/Mined from GenBank, NCBI
57Bothrocarina nigrocaudataNAGBGC6560-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
58Lycogrammoides schmidtiNAGBGC6555-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
59Lycogrammoides schmidtiNAGBGC6556-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
60Lycogrammoides schmidtiNAGBGC6557-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
61Lycogrammoides schmidtiNAGBGC6558-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
62Lycogrammoides schmidtiNAGBGC6559-09Northern Sea of OkhotskNA/Mined from GenBank, NCBI
Outgroup63Bathymaster signatusMIMB 22194FERU014-11Western Bering SeaInstitute of Marine Biology, Vladivostok, Russia/BOLD Systems
Table 2. Measurements of Lycodapus imperatorius sp. nov.
Table 2. Measurements of Lycodapus imperatorius sp. nov.
CharacterHolotypeParatype
TL, mm144132
SL, mm140128
In % of SL
Head length18.918.6
Body depth10.09.4
Body width5.73.9
Predorsal distance21.420.9
Preanal distance36.435.9
Longest dorsal-fin ray5.75.9
Longest anal-fin ray3.93.9
Pectoral-fin length5.76.6
Caudal-fin length2.92.9
Snout length6.16.6
Eye diameter3.13.6
Interorbital width3.63.9
Upper-jaw length8.69.0
Eye to pectoral-fin base9.39.9
Jaw to gill isthmus9.310.0
Gill slit length10.411.1
Upper end of gill slit to upper end of pectoral base00.9
Upper pectoral base to dorsal midline7.15.9
Length of fourth lower gill raker0.50.6
Distance between 4th and 5th lower gill rakers0.90.9
In % of head length
Snout length32.135.2
Eye diameter16.219.3
Interorbital width18.920.9
Upper-jaw length45.348.1
Eye to pectoral-fin base49.153.1
Jaw to gill isthmus49.153.6
Gill slit length54.759.4
Upper end of gill slit to upper end of pectoral base05.0
Upper pectoral base to dorsal midline37.731.4
Length of fourth lower gill raker2.83.4
Distance between 4th and 5th lower gill rakers4.95.0
Pectoral-fin length30.235.6
Caudal-fin length15.115.5
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Prokofiev, A.M.; Balanov, A.A.; Emelianova, O.R.; Orlov, A.M.; Orlova, S.Y. A New Species of Lycodapus from the Emperor Seamount Chain, Northwestern Pacific Ocean (Teleostei: Zoarcidae). Diversity 2022, 14, 972. https://doi.org/10.3390/d14110972

AMA Style

Prokofiev AM, Balanov AA, Emelianova OR, Orlov AM, Orlova SY. A New Species of Lycodapus from the Emperor Seamount Chain, Northwestern Pacific Ocean (Teleostei: Zoarcidae). Diversity. 2022; 14(11):972. https://doi.org/10.3390/d14110972

Chicago/Turabian Style

Prokofiev, Artem M., Andrei A. Balanov, Olga R. Emelianova, Alexei M. Orlov, and Svetlana Yu. Orlova. 2022. "A New Species of Lycodapus from the Emperor Seamount Chain, Northwestern Pacific Ocean (Teleostei: Zoarcidae)" Diversity 14, no. 11: 972. https://doi.org/10.3390/d14110972

APA Style

Prokofiev, A. M., Balanov, A. A., Emelianova, O. R., Orlov, A. M., & Orlova, S. Y. (2022). A New Species of Lycodapus from the Emperor Seamount Chain, Northwestern Pacific Ocean (Teleostei: Zoarcidae). Diversity, 14(11), 972. https://doi.org/10.3390/d14110972

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