Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Heat Stress Assay
2.3. Relative Water Content Determination of L. barbarum Fresh Fruits
2.4. Identification and Chromosomal Location of LbAQP Genes
2.5. Classification of LbAQP Protein Members and Construction of a Phylogenetic Tree
2.6. Structure and Conserved Motif Analysis of LbAQPs
2.7. Promoter Cis-Element Analysis of LbAQPs
2.8. Quantitative Real-Time PCR (qRT-PCR)
2.9. Statistical Analysis
3. Results
3.1. Characterization of the LbAQP Gene Family
3.2. Physical Distribution of LbAQPs on L. barbarum Chromosomes
3.3. Phylogenetic Comparison of LbAQP Proteins of L. barbarum, Tomato, and Arabidopsis
3.4. Gene Structure, Conserved Motifs, and Phylogenetic-Tree-Based Classification of LbAQPs
3.5. Analyzing Cis-Elements in the LbAQP Promoters
3.6. Tissue-Specific Expression
3.7. Expression Profiles of LbAQPs during Fruit Ripening
3.8. Expression Profiles of LbAQPs in Response to Heat Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | CDS Length (bp) | Size (aa) | Size (aa) | PI | TMHMM | GRAVY |
---|---|---|---|---|---|---|---|
LbPIP1;1 | Lba07g01359 | 861 | 287 | 30.777 | 8.31 | 6 | 0.409 |
LbPIP1;2 | Lba06g02040 | 855 | 285 | 30.784 | 8.64 | 6 | 0.318 |
LbPIP1;3 | Lba04g00268 | 858 | 286 | 30.694 | 7.69 | 6 | 0.375 |
LbPIP1;4 | Lba09g02427 | 861 | 287 | 30.866 | 7.68 | 6 | 0.422 |
LbPIP1;5 | Lba09g02372 | 492 | 164 | 17.854 | 9.91 | 3 | 0.746 |
LbPIP1;6 | Lba01g01166 | 858 | 286 | 30.627 | 9.10 | 6 | 0.416 |
LbPIP2;1 | Lba03g00307 | 849 | 283 | 30.223 | 8.21 | 6 | 0.501 |
LbPIP2;2 | Lba03g00306 | 849 | 283 | 30.168 | 6.94 | 6 | 0.496 |
LbPIP2;4 | Lba08g00456 | 861 | 287 | 30.732 | 6.37 | 6 | 0.537 |
LbPIP2;5 | Lba08g01659 | 855 | 285 | 30.425 | 8.24 | 6 | 0.408 |
LbPIP2;6 | Lba06g00111 | 861 | 287 | 30.624 | 8.57 | 6 | 0.590 |
LbPIP2;7 | Lba01g02704 | 528 | 176 | 18.901 | 5.16 | 4 | 0.545 |
LbPIP2;8 | Lba06g03476 | 852 | 284 | 30.435 | 9.28 | 6 | 0.486 |
LbPIP2;9 | Lba08g01171 | 849 | 283 | 30.150 | 9.24 | 6 | 0.512 |
LbPIP2;10 | Lba10g01287 | 852 | 284 | 30.421 | 8.83 | 6 | 0.545 |
LbPIP2;11 | Lba12g01911 | 810 | 270 | 28.867 | 8.82 | 6 | 0.522 |
LbPIP2;12 | Lba05g00065 | 1029 | 343 | 37.897 | 6.40 | 5 | 0.285 |
LbTIP1;1 | Lba01g02671 | 753 | 251 | 25.712 | 5.16 | 6 | 0.740 |
LbTIP2;1 | Lba07g01176 | 744 | 248 | 25.043 | 6.15 | 7 | 0.988 |
LbTIP2;2 | Lba03g02891 | 1476 | 492 | 49.630 | 5.51 | 15 | 1.009 |
LbTIP2;3 | Lba01g01487 | 750 | 250 | 25.220 | 5.35 | 6 | 0.919 |
LbTIP2;4 | Lba01g02017 | 744 | 248 | 24.988 | 5.66 | 7 | 0.952 |
LbTIP3;1 | Lba01g02391 | 774 | 258 | 27.175 | 6.70 | 6 | 0.621 |
LbTIP3;2 | Lba03g01980 | 780 | 260 | 27.752 | 8.07 | 6 | 0.523 |
LbTIP4;1 | Lba04g01403 | 741 | 247 | 25.883 | 6.01 | 7 | 0.866 |
LbTIP5;1 | Lba03g01262 | 768 | 256 | 26.549 | 8.58 | 6 | 0.709 |
LbNIP1;2 | Lba12g01378 | 1614 | 538 | 57.114 | 8.43 | 11 | 0.431 |
LbNIP2;1 | Lba11g02541 | 753 | 251 | 26.644 | 9.20 | 5 | 0.381 |
LbNIP3;1 | Lba01g02539 | 1041 | 347 | 37.614 | 8.45 | 6 | 0.386 |
LbNIP3;2 | Lba07g01497 | 810 | 270 | 29.075 | 9.30 | 5 | 0.564 |
LbNIP4;1 | Lba12g02217 | 834 | 278 | 29.332 | 5.92 | 7 | 0.698 |
LbNIP4;2 | Lba05g02002 | 2859 | 953 | 105.105 | 7.34 | 5 | -0.546 |
LbNIP4;3 | Lba12g00430 | 441 | 147 | 15.815 | 9.61 | 3 | 0.678 |
LbNIP4;4 | Lba12g00428 | 837 | 279 | 29.702 | 7.55 | 6 | 0.702 |
LbNIP5;1 | Lba09g02501 | 1167 | 389 | 40.914 | 6.11 | 5 | 0.249 |
LbNIP6;1 | Lba03g02537 | 1866 | 622 | 64.687 | 8.48 | 12 | 0.421 |
LbXIP1;2 | Lba08g01139 | 975 | 325 | 34.597 | 7.66 | 7 | 0.698 |
LbXIP1;6 | Lba06g03416 | 972 | 324 | 34.684 | 7.04 | 6 | 0.737 |
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He, W.; Liu, M.; Qin, X.; Liang, A.; Chen, Y.; Yin, Y.; Qin, K.; Mu, Z. Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress. Curr. Issues Mol. Biol. 2022, 44, 5933-5948. https://doi.org/10.3390/cimb44120404
He W, Liu M, Qin X, Liang A, Chen Y, Yin Y, Qin K, Mu Z. Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress. Current Issues in Molecular Biology. 2022; 44(12):5933-5948. https://doi.org/10.3390/cimb44120404
Chicago/Turabian StyleHe, Wei, Mingyu Liu, Xiaoya Qin, Aihua Liang, Yan Chen, Yue Yin, Ken Qin, and Zixin Mu. 2022. "Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress" Current Issues in Molecular Biology 44, no. 12: 5933-5948. https://doi.org/10.3390/cimb44120404
APA StyleHe, W., Liu, M., Qin, X., Liang, A., Chen, Y., Yin, Y., Qin, K., & Mu, Z. (2022). Genome-Wide Identification and Expression Analysis of the Aquaporin Gene Family in Lycium barbarum during Fruit Ripening and Seedling Response to Heat Stress. Current Issues in Molecular Biology, 44(12), 5933-5948. https://doi.org/10.3390/cimb44120404