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Article

Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria

by
Malika Semmani
1,
Damien Costa
2,3,
Nassima Achour
4,
Meriem Cherchar
1,
Hanifa Ziane
1,
Abdelmounaim Mouhajir
2,
Venceslas Villier
2,
Haiet Adjmi Hamoudi
5,
Loic Favennec
2,3 and
Romy Razakandrainibe
2,3,*
1
Unité de Parasitologie-Mycologie El Hadi Flici Ex.El Kettar Hospital, Alger 16000, Algeria
2
EA 7510 ESCAPE, Université de Rouen Normandie, 76000 Rouen, France
3
Centre National de Référence-Laboratoire Expert des Cryptosporidioses, Institut de Biologie Clinique, Centre Hospitalo-Universitaire C. Nicolle de Rouen, 76000 Rouen, France
4
Service d’Infectiologie B, EHS El Hadi Flici Ex.El Kettar Hospital, Alger 16000, Algeria
5
Service de Parasitologie-Mycologie Hopital Central De l’Armée, Alger 16000, Algeria
*
Author to whom correspondence should be addressed.
Viruses 2023, 15(2), 362; https://doi.org/10.3390/v15020362
Submission received: 23 November 2022 / Revised: 20 January 2023 / Accepted: 22 January 2023 / Published: 27 January 2023
(This article belongs to the Special Issue HIV and Co-infections: Updates and Insights)
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Abstract

:
The estimated prevalence rate of adults living with HIV infection in MENA is one of the lowest in the world. To date, no data on the genetic characteristics of Cryptosporidium isolates from HIV/AIDS patients in Algeria were available. This study aimed to identify Cryptosporidium species and subtype families prevalent in Algerian HIV-infected patients and contribute to the molecular epidemiology mapping of Cryptosporidium in the MENA region. A total of 350 faecal specimens from HIV/AIDS patients were analysed using microscopy, and a Cryptosporidium infection was identified from 33 samples, with 22 isolates successfully sequencing and confirming species and subtypes. Based on sequence analysis, 15 isolates were identified as C. parvum with family subtypes IIa (n = 7) and IId (n = 8), while five were identified as C. hominis (family subtypes Ia (n = 2) and Ib (n = 3)) and two as C. felis. The C. parvum subtype families IIa and IId predominated, suggesting potential zoonotic transmission. More extensive sampling of both humans and farm animals, especially sheep, goats and calves, as well as a collection of epidemiological data are needed for a better understanding of the sources of human C. parvum infections in Algeria.

1. Introduction

Diarrhoea is usually a symptom of bacterial, viral or parasitic infection and is a leading cause of death in children under five years old worldwide [1,2]. HIV/AIDS and diarrhoea are both major concerns in developing countries. The intestinal tract is one of the hardest-hit organs in HIV-infected individuals. An enterocytic–HIV infection results in enterocyte atrophy, destruction of gut immune cells and intestinal dysfunction leading to diarrhoea [3]. Diarrhoea is a significant cause of morbidity in HIV patients, and nearly 40% of those who die of AIDS experience diarrhoea [4,5,6]. People with HIV/AIDS are at increased risk of contracting diarrhoea. Cryptosporidium is a common cause of diarrhoea in people with HIV/AIDS [7]. This parasitic infection is an opportunistic infection indicator of full symptomatic AIDS. In people with weakened immune systems (HIV-infected people, cancer patients and organ recipients), cryptosporidiosis can be severe, long-lasting and sometimes fatal. Of the 40 currently recognised Cryptosporidium species, Cryptosporidium hominis and Cryptosporidium parvum are responsible for most human infections. However, other species have also been found in immunocompromised patients, including C. meleagridis, C. canis and C. felis [8]. Cryptosporidiosis remains a common cause of chronic diarrhoea in AIDS patients in developing countries, with up to 74% of AIDS patients with diarrhoea harbouring microorganisms in their stool [9].
The Middle East and North Africa (MENA) region encompasses approximately 22 countries that extend from Morocco to Iran. The estimated prevalence rate of adults (aged 15–49 years) living with HIV infection is one of the lowest in the world (less than 0.1%). In Morocco, although no data are available concerning the incidence of cryptosporidiosis in HIV-infected patients, two respiratory cryptosporidiosis cases were reported in this population under high active antiretroviral therapy (HAART) [10]. Algeria has a sustained low-level epidemic (prevalence in the general population in Algeria is less than 0.1%), and rates are higher among migrant and mobile populations, according to the national plan to combat sexually transmitted infections/HIV/AIDS for 2020–2024 issued by the Ministry of Health, Hospitals and Population Reform of Algeria. Available data indicate 11,000 to 14,000 people living with HIV, among whom 68–82% had access to antiretroviral therapy. However, in the country, the late detection of HIV infection and the non-adherence to treatments are individual, social and economic problems in the fight against HIV/AIDS. As with other developing countries, opportunistic intestinal parasites are poorly addressed [11]. With the development of molecular epidemiology, more and more data are available worldwide, enabling a better knowledge of the distribution of Cryptosporidium spp. In Algeria, even though the information is available on the distribution of Cryptosporidium species in animals [12,13,14], there is a lack of data on the prevalence and subtypes of Cryptosporidium spp. circulating in HIV/AIDS patients. This study aimed to characterise Cryptosporidium species and subtypes distribution in HIV-infected patients in Algeria. Not only will the findings generated from this study improve our understanding of the molecular epidemiology of cryptosporidiosis in Algeria, but it will also contribute to the mapping of the epidemiology of Cryptosporidium in the MENA region.

2. Materials and Methods

2.1. Patients, Faeces Sampling and Microscopy

A cross-sectional study was conducted. Faecal sampling was carried out from 2016 to 2018 and obtained from 350 patients with an HIV/AIDS positive status associated with diarrhoea attending inpatient (hospitalisation) and outpatient care units at El Hadi Flici (ex El- Kettar) hospital, Alger city, Algeria. After obtaining informed consent, patients completed a comprehensive questionnaire on age, sex, contact with animals (pets and farm animals) and drinking water sources. Clinical characteristics, including diarrhoea, weight loss, vomiting, abdominal pain and nausea, types of HAART drug regimens and laboratory characteristics, including blood CD4+ T-cell counts, were recorded by the physicians in charge. Cryptosporidium microscopy-based screening was performed in El Hadi Flici Ex El- Kettar hospital, Alger city, Algeria. All specimens were smeared onto glass slides, stained using the modified Ziehl Nielsen (mZN) and auramine techniques [15] and examined using light (1000×) and fluorescence (100× and 400×) microscopy, respectively (Figure 1). A sample was considered Cryptosporidium-positive if typical 4–6 μm diameter oocysts were visible. Positive samples were transferred to the Centre National de Référence–Laboratoire Expert crypyosporidioses (CNR-LE) (Rouen University Hospital, France) for molecular analysis.

2.2. Molecular Characterisation of Cryptosporidium spp.

2.2.1. Identification and Characterisation of Cryptosporidium Species

Cryptosporidium species were screened using 18S rRNA gene, genomic DNAs were subjected to PCR-based sequencing as described by Khoeler et al., 2013 [16]. A two-step nested PCR protocol was used to amplify the 18S rRNA gene (215 bp). For primary PCR, the cycling protocol was 94 °C for 5 min (initial denaturation), followed by 30 cycles of 94 °C for 45 s (denaturation), 45 °C for 2 min (annealing) and 72 °C for 1.5 min (extension), with a final extension of 72 °C for 10 min. For secondary PCR, the protocol was 94 °C for 5 min, followed by 35 cycles of 94 °C for 30 s, 55 °C for 30 s and 72 °C for 30 s, with a final extension of 72 °C for 10 min. C. hominis, C. parvum and no-template PCR controls were included in each run for each protocol.

2.2.2. Gp60 Sequence Amplification

Genotyping was performed by sequencing a fragment of the Gp60 gene. Primers AL3531 and AL3533 were used in the primary PCR and primers AL3532 and LX0029 in the secondary PCR leading to amplification of a fragment of approximately 364 bp [17]. Each PCR mixture (total volume, 50 μL) contained 5 μL of 10× DreamTaq Buffer, each deoxynucleoside triphosphate at a concentration of 0.2 mM, each primer at a concentration of 100 nM, 2.5 U of DreamTaq polymerase and 5µL of DNA template. Additionally, 1.25 µL of DMSO (100%) was added to the mixture. A total of 40 cycles, each consisting of 94 °C for 45 s, 55 °C for 45 s and 72 °C for 1 min, were performed. An initial hot start at 94 °C for 3 min and a final extension step at 72 °C for 7 min was also included. Each amplification run included a negative control (PCR water) and two positive controls (genomic DNA from C. parvum oocysts purchased from Waterborne Inc., and C. hominis genomic DNA from a faecal specimen collected in Rouen University Hospital). Products were visualised in 2% agarose gels using ethidium bromide staining, and identification was confirmed by sequencing. Positive samples were further genotyped by DNA sequencing of the Gp60 gene amplified by a nested PCR following the protocol described elsewhere [18].

2.2.3. DNA Sequence Analysis

Sequencing was used to confirm Cryptosporidium species/genotypes from second-round PCR products. PCR amplicons were purified using Exonuclease I/Shrimp Alkaline Phosphatase (Exo-SAP-IT) (USB Corporation, Cleveland, OH, USA). They were sequenced in both directions using the same PCR primers at 3.2 μM in 10 μL reactions, Big Dye™ chemistries, in ABI 3500 sequence analyser (Applied Biosystems, Foster City, CA, USA). Sequence chromatograms of each strand were examined with 4 peaks software and compared with published sequences in the GenBank database using BLAST (www.ncbi.nlm.nih.gov/BLAST).

2.3. Consent and Ethical Approval

The authors confirm that all the participants were apprised of the aims of the study protocol. For those aged <18 years, consent was obtained from parents or guardians. Participants were also informed of the right to refuse to participate or withdraw from the study at any time without giving any reason. This study was approved by the Ethical clearance committee of the El Hadi Flici Ex El- Kettar hospital.

2.4. Statistical Analysis

The obtained results were presented using tables and charts (descriptive statistics). R statistical software (version 3.6.3), Chi-squared and Fisher’s exact tests were used to check for an association between Cryptosporidium and factors studied. p-values < 0.05 were considered statistically significant.

3. Results

3.1. Clinical Characteristics of Patients

Of individual faecal samples from 350 HIV patients examined for the presence of Cryptosporidium oocysts, 33 (16 female and 17 male patients) were found positive. The median age of these patients was 40 years (range 7–82 years). Reported cases were highest among patients aged 20–50 years (Figure 2). The major clinical symptoms consisted of watery diarrhoea in all patients (chronic in 31, intermittently in two) which may be associated with nausea, vomiting or abdominal pain (n = 32). Moreover, fever, asthenia and weight loss were reported in 8, 16 and 23 patients, respectively. Less frequently, headache or cognitive impairment was associated with Cryptosporidium infection (n = 5). Mean and median values of CD4+ cell counts were 81.65 cells/mm3 and 50 cells/ mm3 (range 1–512 cells/mm3), respectively. Correlation of the Cryptosporidium infection in HIV patients with their CD4+ cell count proved that the patients with CD4 count of <200 cells/mm3 are at higher risk of cryptosporidiosis, with more cases reported below 50 cells/mm3 CD4 count (Table 1).

3.2. Cryptosporidium Species and gp60 Genotypes Distribution

A total of twenty-two of the thirty-three positive isolates were successfully amplified at the 18S rRNA and gp60 locus. Based on sequence analysis, C. parvum was identified in 15 samples with family subtypes IIa (n = 7) and IId (n = 8). C. hominis was detected in 5 cases (family subtypes Ia (n = 2) and Ib (n = 3)) and 2 patients were infected with C. felis. Heterogeneity of Cryptosporidium was observed, eleven subtypes were identified, including seven C. parvum subtypes (IIaA14G2R1, IIaA15G2R1, IIaA16G2R1, IIaA20G1R1, IIaA21G1R1, IIdA16G1 and IIdA19G1) and four C. hominis subtypes (IaA24, IaA22R2, IbA10G2 and IbA13G3). Among C. parvum subtypes, IIdA16G1 and IIdA19G1 had the highest occurrence followed by IIaA15G2R1. For C. hominis isolates, IbA13G3 was identified in 2 specimens and the other subtypes in one sample each (Table 2). Unique sequences generated in this study were deposited in GenBank under accession numbers MT084775-MT084794.

4. Discussion

In many world regions, microscopy-based identification provides a simple, cost-effective and vital method for diagnosing and screening hematologic and infectious diseases. In this study, only microscopically positive samples for Cryptosporidium infection were sequenced and further analysed; however, microscopy is less sensitive, which may underestimate the prevalence of Cryptosporidium infection and lead to some biases in the following results. Although the low rate of positive microscopy results in our study could result from the low sensitivity of microscopy, the slides were independently confirmed by two readers, with any discrepancies resolved by a third reading. We collected one stool sample from each participant, which may result in an underestimate of the true prevalence.
Cryptosporidiosis is a significant cause of chronic diarrhoea and death in HIV/AIDS patients. Diarrhoea occurs in 90% of HIV/AIDS patients in developing countries and about 30–60% in developed countries [19,20]. According to the World Health Organization, classic diarrhoea is generally differentiated into acute and chronic-based depending on its duration. Acute diarrhoea is described as having acute onset and duration of not more than 14 days. In contrast, chronic or persistent diarrhoea is defined as having an onset of more than 14 days. Here, 31 of 33 cryptosporidiosis cases in HIV/AIDS patients are associated with chronic diarrhoea. The gastrointestinal tract is a major site of HIV replication, which results in the massive depletion of lamina propria CD4 T cells during acute infection, which leaves affected individuals mortally susceptible to opportunistic infections. In this study, six patients died from cryptosporidiosis, and two were associated with pulmonary tuberculosis and visceral leishmaniasis. Immunostimulation induced by Leishmania promotes HIV replication and progression to AIDS while decreasing immunity through immune resource depletion [21,22]. Access to highly active antiretroviral therapy (HAART) has significantly reduced the morbidity and mortality caused by cryptosporidiosis [23].
In the literature, it has been shown that treatment with ritonavir results in a drastic reduction in C. parvum infection in vivo (neonatal Balb/c mice) and in vitro (human ileal adenocarcinoma cell line) models [24]. Algeria has provided HAART free of charge since 1998, standing out as one of the countries in the MENA region with the most advanced health responses. In our present findings, the overall prevalence rate of cryptosporidiosis was 9.42% (33/350) among HIV/AIDS patients. Among the 22 patients with GP60-characterised Cryptosporidium spp. infection, nine documented patients reported adherence to HAART and distributed as follows: (1) Four patients initiated first-line ART regimen consisting of a combination between nucleoside analogue reverse transcriptase inhibitors (NRTIs) and non-NRTIs. Lamivudine (3TC) and Efavirenz (EFV) were commonly used as the backbone in first-line therapy. HAART regimen was diverse: 3TC/EFV/Abacavir (ABC) (n = 2), 3TC/EFV/ Didanosine (ddI) (n = 1) and 3TC/EFV/Zidovudine (AZT) (n = 1). The results of the Gp60 subtyping showed one C. hominis Ib family (IbA13G3) and within C. parvum, two IIa family subtypes (IIaA15G2R1 and IIaA21G1R1). (2) Four individuals were using the second-line regimen. The favoured second-line therapy was a double-boosted protease inhibitor combination regimen consisting of Darunavir (DRV) boosted with Ritonavir (RTV) in association with 3TC. Subtypes IbA10G2, IIaA15G2R1, IIaA16G2R1 and IIdA16G1 were detected. (3) C. parvum IIaA15G2R1 was identified in a patient with virologic failure on second-line ART regimen. Virologic failure represents the definition of viral non-suppression (plasma HIV RNA > 1000 copies/mL) used by the WHO Public health approach for low-and middle-income countries. This patient died from dehydration associated with high parasite loads. As for whether it was the first or the second-line regimen, no significant association was found between Cryptosporidium infection and HAART treatment at the species and subtype level. In Tunisia, a neighbouring country, 42/526 included outpatients and inpatients who exhibited Cryptosporidium spp. oocysts in faeces. A total of six of the forty-two positive cases were encountered in HIV/AIDS patients [25]. Higher prevalence rates were reported in other countries, such as Ethiopia, with 43.6% [26]. Even though HIV-infected patients under HAART have a reduced risk of suffering from an opportunistic infection, opportunistic gastrointestinal infection still occurs.
Currently, nitazoxanide is the only proven anti-parasitic therapy for Cryptosporidium infections. Although it has previously been demonstrated that nitazoxanide can improve parasite clearance and shorten the duration of clinical symptoms in adults with AIDS-related cryptosporidiosis [27], other trials could not show any significant benefit [28]. Its limited efficacy in compromised hosts has raised important questions regarding how to manage these patients. Paromomycin and/or azithromycin in conjunction with nitazoxanide have been investigated in double-blind, randomised studies to treat cryptosporidiosis in immunocompromised patients, such as those with HIV/AIDS, and the results were found to be encouraging [29]. Be that as it may, improved medications for the treatment of cryptosporidiosis are desperately needed.
Cryptosporidium spp. infection was reported in patients with advanced immunodeficiency who are on HAART, which may explain their dyspeptic symptoms [30]. In HIV patients, CD4+T cell counts <100 cells/mm3 were associated with susceptibility to the Cryptosporidium infection. CD4+T cell counts help predict the course of Cryptosporidium infection, as in many other opportunistic infections. The lower the CD4 cell count (less than 50), the more severely will the disease occur. In this study, the patients’ mean CD4+ T-cell count was 81.66 ± 98.36 cells/mm3 while the median was 50, which is consistent with the previous reports [31,32] stating that HIV-infected patients can have Cryptosporidium infection even when they are on HAART. Therapeutic intervention leads to recovery of the CD4+T cell count in HIV/AIDS patients. Established C. parvum infection resolution in a murine model requires CD4+Tcells and gamma interferon [33]. The emergence of drug-resistant HIV variants, failure or discontinuation of HAART, and /or the re-emergence of Cryptosporidium spp. infection in these patients should be seriously considered and addressed [34,35].
To our knowledge, this is the first study of Cryptosporidium species and subtypes distribution in HIV/AIDS patients in Algeria. Cryptosporidium parvum was the most common species responsible for cryptosporidiosis. The high diversity of Cryptosporidium parvum subtypes was observed in this study, and the results showed that infections were marked by zoonotic isolates of C. parvum (IIa and IId) subtypes. The most prevalent subtype in the IIa subtype family corresponds to the IIaA15G2R1 (n = 3/15). This subtype is the most dominant subtype infecting especially dairy cattle and has been widely reported in zoonotic infections [18,36]. As a risk factor for human cryptosporidiosis, contact with cattle or consumption of raw milk was suggested to be implicated in neighbouring countries such as Tunisia [37]. Interestingly, this subtype has never been reported in Algeria in cattle or other animals. More investigations should be performed with larger and more representative cattle samples in the country. The IId family was generally considered a sheep and goat subtype, even if it was already encountered in humans [38,39]. The IIdA16G1 subtype (n = 4/15) identified during this study has been recently reported in Algerian sheep [40]. The subtype IIdA19G1 (n = 4) was also detected and reported in goats in Spain [41]; in both HIV-positive patients and pre-weaned dairy cattle in China [9,42] but had never been reported in goats or other animals in Algeria. Analysis of the questionnaire showed that three out of eight patients harbouring IId subtypes stated (i) contact with animals or their excreta (living in rural areas of farmed livestock and as sheep breeder) and (ii) consumption of well water. A truck driver infected with C. parvum IIdA19G1 also noted drinking water from wells as he travelled southward. Some cryptosporidiosis outbreaks have been linked to contaminated water. Water treatment system deficiencies are frequently recognised as a primary cause of outbreaks [43,44,45]. Even the finest systems can be overwhelmed by a large density of oocysts entering the source waters in a short period. The thick-walled oocyst is resistant to environmental conditions and most disinfection methods [46]. In this study, tap water is most drunk by HIV/AIDS patients. Aragon TJ et al., in 2003, showed that tap water is the most likely source of cryptosporidiosis. Indeed, people with AIDS who drank tap water in San Francisco were 23 times more likely to contract cryptosporidiosis than those who drank bottled water [47]. Although surface waters are more vulnerable to direct contamination from sewage discharges and runoff, Cryptosporidium may also be found in groundwater. Hancock et al. (1998) published the research results, including 199 groundwater samples screened for Cryptosporidium. They discovered Cryptosporidium oocysts in 5% of vertical wells, 20% of springs, 50% of infiltration galleries and 45% of horizontal wells.
In Algeria, Cryptosporidium parvum was largely predominant over C. hominis, which was reported as the most frequent species in immunocompromised patients [48,49]. Sequences analysis showed the presence of IaA14, IaA22R2, IbA10G2 and IbA13G3. The IbA13G3 subtype is rarely isolated in humans but has already been reported as imported cases of cryptosporidiosis in Spain [50] and encountered in Peruvian HIV-positive individuals, as well as in Nigeria and Cameroon [51,52].
Potential zoonotic transmission to C. felis (n = 2) was highlighted during this study. C. felis usually affects cats; a patient infected with this species reported, in the questionnaire, close contact with cats and birds and died from neuromeningeal cryptococcosis. In Africa, reports of human infection with C. felis are scarce. C. felis was reported in HIV patients in Ethiopia [26], HIV and non-infected patients in Nigeria [31] and children under 5 years old in Kenya [53]. Anthroponotic transmission of C. felis can occur, particularly in areas with a high incidence of cryptosporidiosis in HIV patients [54].

5. Conclusions

The present study documents the occurrence of Cryptosporidium infection in HIV/AIDS patients in Algeria and the characterisation of Cryptosporidium subtypes. Not only will the findings generated from this study improve our understanding of the molecular epidemiology of cryptosporidiosis in Algeria, but they will also contribute to the mapping of the epidemiology of Cryptosporidium in the MENA region. Future research may be directed by the findings in this study (the predominance of C. parvum subtype families IIa and IId) to compare samples from humans and animals to help investigate sources and routes of transmission. More extensive sampling of both humans and farm animals, especially sheep, goats and calves, as well as a collection of epidemiological data are needed for a better understanding of the sources of C. parvum infections in humans in Algeria.

Author Contributions

Conceived and designed the experiments: M.S., L.F., H.A.H. and R.R.; Performed the experiments: M.S., N.A., M.C., D.C., V.V., H.Z. and R.R.; Analysed the data: A.M., L.F. and R.R.; Contributed reagents/materials/analysis tools: M.S. and H.A.H.; Wrote the paper: M.S., L.F. and R.R. All authors have read and agreed to the published version of the manuscript.

Funding

This work was partly supported by a grant from the Région Normandie (GRR Chimie Biologie Santé, réseau sécurité sanitaire, Grant #16b06801GR). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The authors gratefully thank Santé Publique France for their funding of CNR-LE cryptosporidiosis activities.

Institutional Review Board Statement

According to the French law, verbal approval was required from the patient or their relatives. The study was conducted in accordance with the principles of the Declaration of Helsinki related to human research.

Informed Consent Statement

All the participants were apprised of the aims of the study protocol. For those aged <18 years, consents were obtained from parents or guardians. Participants were also informed of the right to refuse to participate or withdraw from the study at any time without giving any reason.

Data Availability Statement

The original contributions presented in the study are included in the article; further inquiries can be directed to the corresponding authors.

Acknowledgments

The authors are grateful to Nikki Sabourin-Gibbs, Rouen University Hospital, for her help in editing the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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Viruses 15 00362 g001 550
Figure 1. Cryptosporidium oocysts observed from stools (A) by direct wet mount (arrows); (B) mZN staining and (C) auramine–phenol staining.
Figure 1. Cryptosporidium oocysts observed from stools (A) by direct wet mount (arrows); (B) mZN staining and (C) auramine–phenol staining.
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Viruses 15 00362 g002 550
Figure 2. Stacked column chart depicting the age distribution of Cryptosporidium-infected HIV/AIDS patients. The red-coloured numbers represent the number of Cryptosporidium-positive patients, and the blue ones are the negatives. ND: Not disclosed.
Figure 2. Stacked column chart depicting the age distribution of Cryptosporidium-infected HIV/AIDS patients. The red-coloured numbers represent the number of Cryptosporidium-positive patients, and the blue ones are the negatives. ND: Not disclosed.
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Table
Table 1. Association of CD4 count with cryptosporidiosis.
Table 1. Association of CD4 count with cryptosporidiosis.
Cryptosporidiosis
PositiveNegativeTotalp-Value
CD4 count > 200 cells/mm32202204-
CD4 count [50–200] cells/mm3108595<0.001
CD4 count < 50 cells/mm3183048<0.001
CD4 count not available3-3
Total33317350
Table
Table 2. Cryptosporidium subtypes distribution in HIV/AIDS patients in Algeria.
Table 2. Cryptosporidium subtypes distribution in HIV/AIDS patients in Algeria.
SexAge (years)Contact with AnimalsWater ConsumptionDiarrhoeaCD4 (cells/mm3)Cryptosporidium Species Subtypes
F54CatsTap waterChronic26C. parvumIIaA15G2R1
M64Sheep and cattleWell waterChronic45C. parvumIIdA19G1
F30No Tap waterChronic20C. parvumIIdA19G1
M25No Well waterChronic57C. felis
M17Cats and pigeonsTap waterChronicNo sampling C.felis
M41No Well waterChronic1C. parvumIIdA19G1
M48No Tap waterChronic172C. parvumIIdA16G1
F82No responseTap waterIntermittent512C. parvumIIaA21G1R1
FNRNo Not specifiedChronicNo sampling C. parvumIIdA19G1
M60No responseTap waterChronic55C. parvumIIaA20G1R1
F44No responseNot specifiedChronic16C. parvumIIdA16G1
F58No responseTap waterChronic40C. parvumIIaA16G2R1
M38No responseTap waterChronic178C. parvumIIaA15G2R1
M41Sheep and cattle (Sheep breeder)Well waterChronic92C. parvumIIdA16G1
MNRNo responseNot specifiedChronic207C. parvumIIaA15G2R1
M66Living in rural areas of farmed livestockTap waterIntermittentNo sampling C.hominisIaA14
F37No Bottled waterChronic9C.hominisIbA10G2
F30No Tap waterChronic50C. parvumIIaA14G2R1
F28No Tap waterChronic109C.hominisIbA13G3
FNRNo Tap waterChronic47C.hominisIbA13G3
M7No Tap waterChronic7C.hominisIaA22R2
M42Sheep and cattle (Sheep breeder)Well waterChronic53C. parvumIIdA16G1
NR: not reported.
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Semmani, M.; Costa, D.; Achour, N.; Cherchar, M.; Ziane, H.; Mouhajir, A.; Villier, V.; Adjmi Hamoudi, H.; Favennec, L.; Razakandrainibe, R. Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria. Viruses 2023, 15, 362. https://doi.org/10.3390/v15020362

AMA Style

Semmani M, Costa D, Achour N, Cherchar M, Ziane H, Mouhajir A, Villier V, Adjmi Hamoudi H, Favennec L, Razakandrainibe R. Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria. Viruses. 2023; 15(2):362. https://doi.org/10.3390/v15020362

Chicago/Turabian Style

Semmani, Malika, Damien Costa, Nassima Achour, Meriem Cherchar, Hanifa Ziane, Abdelmounaim Mouhajir, Venceslas Villier, Haiet Adjmi Hamoudi, Loic Favennec, and Romy Razakandrainibe. 2023. "Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria" Viruses 15, no. 2: 362. https://doi.org/10.3390/v15020362

APA Style

Semmani, M., Costa, D., Achour, N., Cherchar, M., Ziane, H., Mouhajir, A., Villier, V., Adjmi Hamoudi, H., Favennec, L., & Razakandrainibe, R. (2023). Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria. Viruses, 15(2), 362. https://doi.org/10.3390/v15020362

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