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Volume 17
Issue 2
10.3390/v17020165
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Immunogenic Potential of Selected Peptides from SARS-CoV-2 Proteins and Their Ability to Block S1/ACE-2 Binding

by
Lara Cristina da Silva Lima
1,2,†,
Thiago Demetrius Woiski
1,†,
Juliana Ferreira de Moura
3,
Roberto Rosati
1,2,
João Carlos Minozzo
4,
Emeline Huk da Silva
1,
Aline Castro Rodrigues Lucena
1,2,
Bruno Cezar Antunes
4,
Sérgio Caldas
5,
Myrian Morato Duarte
5,
Maurício Abreu Santos
5,
Rubens Luiz Ferreira Gusso
4,
Erickson Luiz de Moura
4,
Ana Paula Santos Silva
4,
Luciana Potzecki
4,
Daniele Maria Ferreira
1,2,
Elizabeth Soares Fernandes
1,2,
Bonald Cavalcante de Figueiredo
1,2 and
Lauro Mera de Souza
1,2,*
1
Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80035-000, PR, Brazil
2
Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil
3
Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba 81531-980, PR, Brazil
4
Centro de Produção e Pesquisa de Imunobiológicos, Piraquara 83302-200, PR, Brazil
5
Diretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte 30510-010, MG, Brazil
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2025, 17(2), 165; https://doi.org/10.3390/v17020165
Submission received: 30 September 2024 / Revised: 10 January 2025 / Accepted: 15 January 2025 / Published: 24 January 2025
(This article belongs to the Special Issue Basic Sciences for the Conquest of COVID-19)
Review Reports Versions Notes

Abstract

The first infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes the coronavirus disease 2019 (COVID-19), occurred in December 2019. Within a single month, the disease reached other countries, spreading in a rapid and generalized manner worldwide to cause the COVID-19 pandemic. In Brazil, the number of COVID-19 cases surpassed 38 million. This study was conducted to produce antibodies against SARS-CoV-2 and investigate the immunogenic potential of synthetic peptides containing partial sequences of the main proteins (spike, membrane, and nucleocapsid proteins). In addition, we evaluated the ability of the antibodies to impair the interaction between the spike S1 protein and human ACE-2 protein, which is the main route of entry of the virus into host cells. By immunizing horses with synthetic peptides, we obtained hyperimmune sera with specific anti-SARS-CoV-2 antibodies, which were fragmented to release the F(ab’)2 portion that binds to the different SARS-CoV-2 proteins as a recombinant S1-protein and proteins from a viral lysate. The other F(ab’)2 samples also impaired the interaction between S1 protein and ACE-2 proteins, showing high potential to prevent viral spreading.
Keywords: SARS-CoV-2; COVID-19; immunization; antibody; F(ab’)2; viral neutralizing SARS-CoV-2; COVID-19; immunization; antibody; F(ab’)2; viral neutralizing

Share and Cite

MDPI and ACS Style

da Silva Lima, L.C.; Woiski, T.D.; de Moura, J.F.; Rosati, R.; Minozzo, J.C.; da Silva, E.H.; Lucena, A.C.R.; Antunes, B.C.; Caldas, S.; Duarte, M.M.; et al. Immunogenic Potential of Selected Peptides from SARS-CoV-2 Proteins and Their Ability to Block S1/ACE-2 Binding. Viruses 2025, 17, 165. https://doi.org/10.3390/v17020165

AMA Style

da Silva Lima LC, Woiski TD, de Moura JF, Rosati R, Minozzo JC, da Silva EH, Lucena ACR, Antunes BC, Caldas S, Duarte MM, et al. Immunogenic Potential of Selected Peptides from SARS-CoV-2 Proteins and Their Ability to Block S1/ACE-2 Binding. Viruses. 2025; 17(2):165. https://doi.org/10.3390/v17020165

Chicago/Turabian Style

da Silva Lima, Lara Cristina, Thiago Demetrius Woiski, Juliana Ferreira de Moura, Roberto Rosati, João Carlos Minozzo, Emeline Huk da Silva, Aline Castro Rodrigues Lucena, Bruno Cezar Antunes, Sérgio Caldas, Myrian Morato Duarte, and et al. 2025. "Immunogenic Potential of Selected Peptides from SARS-CoV-2 Proteins and Their Ability to Block S1/ACE-2 Binding" Viruses 17, no. 2: 165. https://doi.org/10.3390/v17020165

APA Style

da Silva Lima, L. C., Woiski, T. D., de Moura, J. F., Rosati, R., Minozzo, J. C., da Silva, E. H., Lucena, A. C. R., Antunes, B. C., Caldas, S., Duarte, M. M., Santos, M. A., Gusso, R. L. F., de Moura, E. L., Silva, A. P. S., Potzecki, L., Ferreira, D. M., Fernandes, E. S., de Figueiredo, B. C., & de Souza, L. M. (2025). Immunogenic Potential of Selected Peptides from SARS-CoV-2 Proteins and Their Ability to Block S1/ACE-2 Binding. Viruses, 17(2), 165. https://doi.org/10.3390/v17020165

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