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Review

Influence of Nitrate Supplementation on Endurance Cyclic Sports Performance: A Systematic Review

by
Jorge Lorenzo Calvo
1,*,
Francesca Alorda-Capo
2,
Helios Pareja-Galeano
2 and
Sergio L. Jiménez
2,*
1
Sport Department, Facultad de Ciencias de la Actividad Física y del Deporte, Universidad Politécnica de Madrid, 28040 Madrid, Spain
2
Faculty of Sport Sciences, Universidad Europea De Madrid, 28670 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Nutrients 2020, 12(6), 1796; https://doi.org/10.3390/nu12061796
Submission received: 28 March 2020 / Revised: 11 June 2020 / Accepted: 12 June 2020 / Published: 17 June 2020
(This article belongs to the Special Issue Nitrate Supplementation for Performance and Health)
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Abstract

:
Endurance can be defined as the capacity to maintain one’s velocity or power output for the longest possible time. Maintaining such activity can lead to the onset of fatigue. Dietary nitrate supplementation produces an ergogenic effect due to the improvement of mitochondrial oxygen efficiency through a reduction in the oxygen cost of exercise that increases vasodilation and blood flow to the skeletal muscle in recreationally active subjects. However, the effects of dietary nitrate supplementation on well-trained endurance athletes remain unclear; such supplementation could affect more performance areas. In the present study, a systematic review of the literature was conducted to clarify the use and effects of nitrate as a dietary supplement in endurance athletes trained in cyclic sports (repetitive movement sports). A systematic search was carried out following the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines in the databases of SCOPUS, Web of Science (WOS), Medline (PubMed), and Sport Discus from 1 January 2010 to 30 November 2019. Twenty-seven studies were included in the study. The methodological quality of the articles was assessed using the McMaster Critical Review Form. Statistically significant ergogenic results were obtained in 8 (29.63%) of the 27 studies investigated, with significant results obtained for cardiorespiratory parameters and performance measures. Improvement in exercise tolerance was obtained, which could help with exhaustion over time, while the improvement in exercise economics was not as clear. Additionally, the dose necessary for this ergogenic effect seems to have a direct relationship with the physical condition of the athlete. The acute dose is around 6–12.4 mmol/day of nitrate administered 2–3 h before the activity, with the same amount given as a chronic dose over 6–15 days. Further studies are required to understand the factors that affect the potential ergogenic impacts of nitrate on athletic performance among endurance athletes.

1. Introduction

Nitrate supplementation enhances nitric oxide (NO) bioavailability via the NO3–nitrite–NO pathway, which is involved in several physiological processes that could potentially improve skeletal muscle function [1,2]. NO endogenous synthesis occurs through at least two different physiological pathways: the NO synthase (NOS)-dependent and NOS-independent pathways [2]. L-arginine and L-citrulline are the main precursors of NO in the NOS-dependent pathway, while nitrate (NO3−) and nitrite (NO2−) are the main substrates that produce NO via the NOS-independent pathway (Figure 1).
In addition to the endogenous generation of NO through the NOS pathway, NO3− and NO2− reserves can also be increased exogenically through one’s diet. Approximately 80% of the nitrates in one’s diet come from the consumption of vegetables [3], mainly through green-leaf vegetables, such as lettuce, spinach, arugula, celery, watercress, and beet. Beet is one of the main nitrate sources since it contains 250 mg (4 mmol) of nitrate per 100 g of fresh weight (Table 1).
NO signaling affects multiple physiological mechanisms that could potentially enhance skeletal muscle. NO mediates smooth muscle relaxation, which promotes vasodilation and blood flow regulation and thereby improves oxygen delivery and mitochondrial respiration (Figure 1). This, in turn, enhances type II muscle fiber function [5,6,7]. This physiological facilitation suggests that supplementation with nitrates could have ergogenic effects on cardiorespiratory endurance [8].
The ergogenic effects of nitrate have been demonstrated in endurance and submaximal exercises to improve high-intensity endurance performance [8,9]. This has led the scientific community to increase the number of investigations that analyze the ergogenic potential of nitrates under different exercise conditions, including high to low intensity, long to short duration, continuous and intermittent, normoxia and hypoxia, and acute dose and chronic exposure, with different nitrate sources and doses [10,11,12,13]. Supplementation with nitrates, either with pure nitrate salts or foods rich in nitrates, has been proven to enhance the sporting performance of recreationally active young people [14,15,16,17], while nitrate supplementation has not yet been shown to improve performance among non-professional athletes [18].
Endurance cyclic sports require repetitive movements, usually lasting more than five minutes, through predominantly aerobic metabolism. When an endurance athlete takes part in high-intensity effort, various physiological factors limit their performance, such as the maximum oxygen uptake, first and second ventilatory threshold, and energy efficiency and economy [8]. At the same time, these factors can potentially be improved through NO signaling via nitrate supplementation.
The objective of this systematic review was to analyze the ergogenic effect of nitrate supplementation on well-trained cyclic sports athletes, as well as to study the optimal sources and doses of nitrates.

2. Materials and Methods

2.1. Eligibility Criteria

This systematic review focused on the effect of nitrate supplementation on endurance cyclic sports performance. This review followed the PICOS question model for framing the research question and defining the inclusion criteria [19]: P (population): “endurance and healthy trained athletes between 18 and 50 years old”; I (intervention): “supplementation with nitrate”; C (comparison): “same conditions with placebo or control group”; O (outcomes): “performance measures”; S (study design): “double- or single-blind design and randomized parallel or crossed.”

2.2. Literature Search

A structured search was carried out following the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines [20] in the SCOPUS, Web of Science (WOS), Sport Discus, and Medline (PubMed) databases. The search included original articles with randomized controlled crossover or parallel designs in which the intake of nitrate administered before and/or after the exercise was compared with an identical placebo situation. Due to the increasing number of investigations over the last 10 years interested in using nitrate as a supplement in athletes, the search was bounded from 1 January 2010 to 30 November 2019. Search terms were established matching the Medical Subject Headings (MeSH), as follows: “nitrate,” “nitric oxide,” “beetroot juice,” “performance exercise,” and “endurance.” The connectors OR and AND were also used to filter the search.

2.3. Study Selection

The following inclusion criteria were applied to select studies: (I) articles depicting a well-designed experiment that included the ingestion of a dose of nitrate, or nitric oxide or beetroot juice before and/or during exercise in healthy trained athletes; (II) the study investigated endurance cyclic sports; (III) there was an identical experimental situation with or without the ingestion of a placebo in a normoxic condition; (IV) there was a double- or single-blind design and randomized parallel or crossed design; (V) crossed over studies that did not allow for a clean-up period of over 24 h were excluded; (VI) there was clear information on the administration of nitrate; (VII) nitrate was administered in the form of a beverage, gum, or pills; (VIII) at least one of the measured performance variables was changes (effort shown, maximum power, average power, time, distance, oxygen consumption, saturated oxygen, total work, or number of completed sprints among others); and (IX) the language was restricted to English. The following exclusion criteria were applied: (I) animal studies, (II) uncontrolled trials, (III) studies using non-standardized turmeric extracts or extracts of unknown nitrate content, (IV) studies performed on non-trained or injured/sick subjects, (V) studies investigating children and the elderly, and (VI) studies in non-specific sports or non-endurance sports.

2.4. Data Extraction

The following information was extracted from the selected articles: study source (authors and year of publication), participant characteristics (level of activity or sports discipline, experience and training load described by the authors, number of participants and sex), age, VO2max, supplementation protocol (source of nitrates and duration), nitrate dose, time from the last dose of nitrate, exercise protocol, main outcome (performance measures), and results.

2.5. Quality Assessment and Risk of Bias

The methodological quality of the articles, evaluated using McMaster’s Critical Review Form [21], ranged between 13 and 15 points, representing a minimum methodological quality of 86.6% and a maximum of 100%. Of the 27 studies, 3 achieved 13 items, representing 11.11% of the total number of studies, 3 achieved 14 items, representing the same percentage of 11.11%, and the rest of the studies (21) achieved 15 items, representing 77.78% of the total number of studies. All studies achieved a “very good” quality. No study was excluded because it did not reach the minimum quality threshold. The main deficiencies found in methodological quality were associated with items 13 and 14 of the questionnaire and comprised the report of clinical importance and one study had drop-outs. The objective of this evaluation was to determine and to compare the quality between the different study designs (Table 2).

3. Results

3.1. Study Selection

The initial research obtained through database searches provided 4341 records. Of these records, 3102 duplicates were removed. Of the 1239 remaining records, a total of 1067 were excluded after screening the titles and abstracts for eligibility. Ultimately, 27 studies were included in this review. The PRISMA flow chart was used to summarize the systematic research and selection process (Figure 2).

3.2. Characteristics of the Studies

A total of 323 participants were included in these 27 studies. The average sample included 12 subjects but seven studies assessed the effects of nitrate in larger group subjects (up to 26 participants). The study mode was carried out using eight subjects. All subjects in the review were defined by the authors as either trained or well-trained using the various criteria listed in Table 3. Among the 323 experimental subjects, only 39 (12.07%) were women. Moreover, female athletes only took part in 5 of the 27 studies selected, with only one study exclusively on trained women. Most of the studies were performed with populations in their 20s and 30s. Most focused on cycling, with 9 studies (33.33%) and 101 participants (31.27%), followed by combined cycling and triathlon studies, with 97 participants (30%) and 6 studies (22.2%). We also found other less represented sports, such as swimming (3), athletics (2), rowing (1), and cross-country skiing (2). For the ergogenic effect of nitrate supplementation, statistically significant results were obtained in eight (29.63%) of the studies that investigated cyclic endurance sports performance [22,23,26,33,35,44,45].
The main form of supplementation was beetroot, except for three studies that used sodium nitrate [18,23,40] and one that used potassium nitrate [42]. Most of the studies used mmol as a measuring unit to determine the value of the dose of nitrate intake. The average dose of nitrate was 8.7 mmol/day (minimum 4 mmol/day and a 19.5 mmol/day maximum). Both acute and chronic supplementation protocols were found in the reviewed studies. Of the studies, 40.74% used chronic supplementation and 51.85% used acute supplementation, applying equally high and low doses for both acute and chronic treatments, and 7.4% used both types of supplementation.
Studies on chronic treatment were performed over an average of 6 days, taking into account the fact that some treatments lasted 3 days, and others, such as those described by Balsalobre et al. [22], applied supplementation for 15 days. The last dose of nitrate before physical performance testing was not given at the same moment in all the studies. The intervals used for administering the dose before the performance test was between 1.5 and 4 h, with the most common being 2–3 h (88.89%). Only the study of Balsalobre et al. [22], which featured the longest chronic supplementation (15 days), began the incremental test until exhaustion 24 h after the last dose.
Athletes in this systematic review had a moderate-to-high level of cardiorespiratory fitness. Most of the studies determined the VO2max of their athletes. The VO2max values ranged from 42 mL/kg/min to 85 mL/kg/min, with the lowest values found in studies using male master athletes in swimming [43]. Other measures of performance used to determine the effects of nitrate supplementation were time, distance average, and maximum power. Moreover, except for some gastrointestinal pain, secondary effects were not reported, and all doses of supplementation were tolerated. For example, in the study by Hoon et al. [33], two athletes described mild gastrointestinal pain after consuming a nitrate-rich drink.

3.3. Risk of Bias

Most of the studies did not present a risk of bias. They were carried out using healthy and uninjured athletes via a randomized double-blind control test, leaving a sufficiently long clean-up period lasting from 4 to 7 days (except for some that did not use a crossover). Approximately 10% were done using simple blind tests. The placebo drinks, in most cases, had the same packaging, color, smell, and taste as the nitrate-rich drinks to reduce the risk of bias.
Most studies gave clear instructions to participants to refrain from caffeine and alcohol intake for at least 24 h before performance testing. Further, identical diets were used in the experiments such that diet would not influence the results. Details of nitrate-rich foods (green vegetables, beets, strawberries, grapes, and tea) and the importance of eliminating them from one’s diet during the period of experimentation were given. Most participants maintained a moderate level of training during the previous days, in addition to refraining from physical exercise during the last 24 h before testing.

3.4. Group and Subgroup Effects

In all studies, the concentrations of nitrate and plasma nitrite increased significantly (p < 0.05) after nitrate supplementation compared to the placebo. Among the 27 studies reviewed, about 29.63% provided results that were statistically significant for nitrate intake. As shown in Table 4, of the eight articles, four showed significant results for both respiratory parameters and measures of performance [22,23,43,44], seven studies give results for measures of performance [22,26,33,35,43,44,45] of which three did not influence respiratory parameters [33,35,45], and five significant studies gave results on respiratory parameters [22,23,26,43,44], of which only one did not influence the measures of performance [23].
Boorsma et al. [24] used the highest acute (13 mmol) and chronic (19.5 mmol) nitrate doses of all the studies. Nevertheless, the two experimental tests (the incremental test and 1500 m timed trial) provided no significant results. Bescós et al. [23] demonstrated a significant improvement in oxygen consumption during their tests, but in neither case did this improvement increase sporting performance. The other studies showed that nitrate supplementation did not increase VO2 or improve performance.

4. Discussion

4.1. Forms of Nitrate Supplementation

This systematic review demonstrates that the ergogenic effects of nitrate supplementation on endurance cyclic sports performance occur mainly through the intake of beetroot juice. However, the consumption of beetroot juice allows for a substantial nitrate load to be ingested quickly and easily before training or completion. Beetroot juice is considered an ergogenic supplement with strong scientific evidence, according to the ABCD classification of the Australian Sports Institute (AIS) [8]. In the included investigations, a placebo drink with an insignificant nitrate content was used. In addition to nitrate, beetroot also contains several active compounds that can influence physiological responses to exercise, including betaine, antioxidants, and polyphenols [35]. The use of beetroot depleted in nitrate as a placebo allowed the effects of nitrate to be isolated from those of other potentially ergogenic components.
Other studies that applied different forms of nitrate supplementation, such as potassium nitrate or sodium nitrate, did not obtain significant results. Thus, this form of supplementation may be less effective than beetroot juice. For example, a study conducted in 2016 compared the effect of beetroot juice and sodium nitrate on performance in trained athletes and discovered that the same concentration of nitrate produced a larger reduction of oxygen uptake in the experimental group that used beet juice [3], but the study did not highlight any factors related to sports performance.
Balsalobre et al. [22], after supplementing runners with a dose of 6.5 mmol/day of nitrate for 15 days, performed an incremental test until exhaustion on a treadmill (from low to high intensity), showing significant results in time until exhaustion (S: 1269 ± 53.6 vs. PLA: 1230 ± 73.5 s), which means that the supplemented athletes performed better. The most intense part of the test was used to determine the speed associated with VO2max.
Similarly, the incremental test with a cycloergometer performed in a study on cyclists by Bescós et al. [23] demonstrated better results with an acute dose of 11.8 mmol of nitrate compared to a placebo, but these results were not statistically significant (S: 416 ± 32 vs. PLA: 409 ± 27 s). The main difference between both studies was the supplementation protocol (beetroot juice for 15 days and sodium nitrate for a single day). As previously noted, among the 27 studies, 40.74% used a chronic dose and the other 51.85% used an acute dose, making it difficult to determine the option with the best effect. Boorsma et al. [24] and Nyakayiru et al. [40] were the only studies that investigated both types of supplementation and obtained similar results for both acute and chronic supplementation (both negative results). Similarly, McQuillan et al. [38] performed four time trials using different days of supplementation (3, 4, 6, and 7 days), and all of them gave negative results. On the other hand, Cermak conducted two studies [26,27] with triathletes and cyclists with a similar VO2max, dose of nitrate, time between the last intake, and time trial. The only differences were the days of supplementation (6/1 day) and the total time taken for the time trial (16/60 min). Cermak et al. [26] found a significant improvement in performance, while the other study did not obtain better results for the test time. This could be due to the form of supplementation or the type of test done. Notably, the eight most significant studies [22,23,26,33,35,43,44,45] examined five different sports with different doses of supplementation (see Table 3 and Table 4); therefore, the best mode of supplementation to obtain superior performance among athletes remains uncertain.

4.2. Pharmacokinetics of Nitrate

In humans, before absorption through the stomach wall, approximately 25% of the nitrate consumed is absorbed by the salivary glands and reduced to nitrite via the action of nitrate reductase enzymes from the facultative anaerobic bacteria on the dorsal surface of the tongue. Next, this ingested nitrite is reduced to NO in the acidic environment of the stomach but a substantial amount undergoes systematic circulation as nitrite. Plasma nitrate levels increase rapidly within 30 min after supplementation, reaching the peaks of nitrate and nitrite at 1–2 h. Two to three hours later, nitrite and nitrate gradually fall, returning to the baseline after approximately 24 h. The average life of plasma nitrate in humans is approximately 5 h, with a considerable decrease 4 h after ingestion.
All studies that obtained favorable ergogenic effects from nitrate performed their experimental tests between 2 and 3 h after intake. The studies that started their tests before 2 h improved their exercise capacity, while those that started after 3 h failed to improve their exercise capacity [24,25,32,34,37,39,40]. Interestingly, only one study analyzed the effects of the supplement 24 h after intake, obtaining significant results [25].
The nitrate and nitrite levels in plasma when fasting are 20–50,000 mmol/L and 100–500 mmol/L, respectively. Additionally, estimations of the average consumption of nitrate and nitrite in Europe are 31–185 mg/day, with 100% bioavailability of nitrate in the diet [4]. Thus, the doses used in nitrate studies are 4 to 12 times greater than the typical daily intake of nitrate in Europe. Studies like that of Bescós et al. [23] used an acute intake of 10 mg per kg of body weight of sodium nitrate, representing an approximate nitrate dose of 11.8 mmol. With these values, the participants went from having similar nitrate concentrations before intake (S: 30 ± 12, PLA: 28 ± 10 µM) to having an increase in plasma nitrate levels three hours after intake in the experimental group (250 ± 80 µM), with no change observed in the placebo group (29 ± 8 µM).
Amongst the protocols used during the studies, those that decided to refrain from the use of mouthwash or chewing gum stand out. These hygienic products alter the bioavailability of nitrite by killing the oral bacteria necessary for nitrate’s bioactivation. A cross-study designed with seven healthy volunteers examined the effects of antibacterial mouthwash on the nitrite levels in saliva and plasma measured after the intake of sodium nitrate (10 mg/kg) [49]. In the control situation, the levels of nitrate and nitrite in saliva and plasma increased long after the intake of nitrate. Similarly, the use of mouthwash 15 min before the intake of sodium nitrate did not have an effect on the nitrate accumulation in saliva and plasma but prevented its conversion to nitrite in saliva and attenuated the increase in plasma nitrite. The removal of some oral bacteria with mouthwash alters the bioavailability of nitrite in plasma by limiting the bioactivation of nitrate in the mouth.
The eight studies that obtained a performance improvement via nitrate supplementation prohibited the use of mouthwash among the athletes until the experimental tests were carried out. Some tests prohibited the use of mouthwash during the entire period of supplementation [22], while others prohibited it during the last 24 h before the experimental test or from the last intake until the end of the test. On the other hand, studies such as Bescós et al. [18] and Lane et al. [34], which did not see a positive effect in the performance tests after the intake of sodium nitrate and beetroot juice, did not inform the athletes of the need to refrain from oral antibacterial products, which could have caused a smaller increase in NO.

4.3. Exercise Type and Ergogenic Response

Bescós et al. [23], through the administration of large amounts of dietetic nitrate to competitive cyclists and triathletes, determined that even with no improvement in cardiorespiratory adaptation to low and medium exercise intensity (submaximal workloads corresponding to 2.0, 2.5, 3.0, and 3.5 W·kg−1 of body mass), the consumption of oxygen was much more efficiently reduced under high-intensity exercise. This highlights the importance of high-intensity exercise in the performance of cyclic sports.
The ergogenic effect of the beetroot juice was mediated by the influence of NO in the improvement of the mitochondrial oxygen efficiency, which increased vasodilation and muscular blood flow, among other factors related to exercise performance [50]. One of the effects produced by these physiological mechanisms is an improvement of the running economy, which translates into being able to run at the same pace with less effort or being able to run faster with the same energy consumption. In this systematic review, a total of 27 trials were carried out to verify the improvement of endurance through nitrate supplementation. Among them, seven trials showed significant improvement in the time of distance traveled [26,33,35,44,45]. In most of the studies that measured cyclists and triathletes with cycloergometers, different tests of 1 km up to 80 km were employed. Two studies applied a 10 km test and obtained a significant improvement in the time performance and average power when the athletes ingested the supplement within 3 h before starting the test [26,44]. It should be noted that the study by Rokkedal et al. [44] applied the same distance with a shorter time and lower oxygen consumption, which means that the athletes performed at a higher intensity.
On the other hand, Nyakayiru et al. [40] and Shannon et al. [45] did not find a significant improvement in time with the 10 km test, an acute dose, or a chronic 6-day trial. This could be due to the expected time between intake and the start of the test (≥3 h) since the ingested doses and VO2max levels of the cyclists and triathletes in both studies were very similar. However, more studies are necessary to confirm this result.
Studies conducted with distances less than or greater than 10 km did not obtain any significant improvement in exercise capacity [18,25,27,28,31,32,37,38,39,40], except for a study where the yield was verified at 4 and 16.1 km with an acute dose of 6 mmol of beet juice and an improvement in time, and the average power was obtained [35]. This research, even using an acute intake, obtained highly beneficial results, with a time improvement of 2.7% in the 4 km race and 2.8% in the 16.1 km race. Meanwhile, acute doses of nitrate given to trained cyclists for cycling races of 80 km or more seemed to have no ergogenic effect [46]. These results suggest that, as distances become greater, the importance of intensity as a determining factor in performance decreases.
The only study on rowers showed a positive effect on the time required to complete the 2000 m test (via a remover gauge) when using a dose of 8.4 mmol of nitrate, while with a reduced dose (4.2 mmol of nitrate), this effect was not observed [33]. This study demonstrates the importance of applying a higher dose such that the NO level can have an ergogenic effect. Additionally, a 2000 m race is a test that rowers are highly accustomed to; they perform such a race monthly since it is part of the national rowing selection protocol.
The mechanism by which nitrates improve the efficiency of submaximal exercise remains contentious. It is believed that increasing NO levels after supplementation can reduce the cost of ATP production, thereby improving energy production efficiency [51]. This effect is believed to be related to a lower production cost of mitochondrial ATP [6] or muscle contraction ATP [52]. It is known that one of the costliest energy processes during skeletal muscle contractions is the pumping of calcium into the sarcoplasmic reticulum, which accounts for up to 50% of the total ATP turnover. In this way, the efficiency of oxidative phosphorylation is improved under a given relative intensity and the decomposition of phosphocreatine is reduced. In this systematic review, we found 10 investigations that evaluated whether an improvement in VO2 is observable using incremental tests at different levels of VO2max or maximum power (Wmax). Four studies that investigated incremental tests until exhaustion obtained significant results for sports performance [22,43], except for one study that found significant results in respiratory parameters but no differences in the duration of incremental exercise between the experimental group and the placebo group [23]. This was the only study that used an acute dose, which could be one of the attributable causes. These findings show that, in athletes with a high level of training, nitrate supplementation may lead to a greater tolerance of submaximal effort, while at the same time casting doubt on the decrease in oxygen consumption. Other physiological parameters, such as VCO2 or mean heart rate, showed no significant differences between groups; neither did the perceived exertion. Only the study by Balsalobre et al. [22] showed significant improvements in the rating of perceived exertion (RPE).
A study by Pinna et al. [43] with trained male swimmers is a clear example of improved exercise efficiency. The training frequency of swimmers ranged from 3 to 4 times/week, with a 3000–5000 m distance covered each workout. With supplementation of 5.5 mmol of nitrate for six days, an incremental test was carried out until exhaustion in the pool using an elastic cord connected to a digital dynamometer tied on a belt to the subject to study the workloads. The results showed that, through the intake of beet juice, a greater workload and lower aerobic energy consumption were achieved, together with the same VO2.

4.4. Performance Level and Ergogenic Response

While some studies reported that dietary supplementation with NO donors induced benefits in exercise performance, others found no positive effects. In this sense, the training status of the subjects seemed to be a determining factor. Studies with healthy untrained or moderately trained subjects showed that NO donors could improve their tolerance to aerobic and anaerobic exercise [14,15,16,17]. However, for high-level athletes, who are more adapted to training and exercise, nitrate supplementation seemed to have little to no effect, at least when the doses were equal to those used on recreational athletes [53]. Additionally, some studies found that the basal plasma nitrite levels of elite athletes were higher than those of recreational athletes, showing that plasma nitrite is directly proportional to exercise capacity [54]. Therefore, one’s aerobic level could affect the ergogenic benefits induced by dietary nitrate supplementation, and higher doses may be necessary to obtain a benefit for elite athletes. Additionally, the same doses of nitrate among elite and recreational athletes were shown to produce the same increases in plasma nitrate concentrations [55]. The ingestion of a moderate amount of inorganic nitrate might not lead to higher performance because the increase in free radicals of the nitrate–nitrite–NO pathway in well-trained subjects engaging in high-intensity exercise enhances the function of the endogenous antioxidant system of these athletes; consequently, there is a greater reduction in these free radicals. On the other hand, because they are not as heavily trained, recreationally active subjects lack adaptations and may be more exposed to oxidative damage when performing high-intensity sports. Thus, increasing the bioavailability of NO through the intake of nitrate could be a benefit for such athletes. Additionally, the benefits of nitrate supplementation may also depend on individual genetics or the effects of training on muscle oxygenation, mitochondrial function, and muscle fiber type composition. It has been suggested that nitrate supplementation may be particularly effective in improving the physiological and functional responses in type II muscle fibers [56].
It is assumed, in addition to the greater adaptation that athletes have to high-intensity sports, that some athletes will benefit more than others and that there may be responders and non-responders in a given group. In the study by Bescós et al. [18], seven subjects showed a small increase (<30%) in plasma nitrite levels after dietary supplementation with nitrate compared to placebo. These subjects were methodologically classified as “low responders” or “high responders.” Although no statistical differences were found in the performance parameters (distance and output power) between the two groups, during the 40-min time trial after treatment, there was a small reduction in VO2 (≈1.7%), VCO2 (≈3.4%), and the relationship between VO2 and power (≈1.1%) in the group of high responders. Additionally, blood lactate concentrations showed a tendency to decrease at 3 min after exercise (p = 0.076) in most responding patients. Similarly, even without being able to demonstrate a significant effect in the 1500 m race, two runners responded positively to acute supplementation of 19.5 mmol nitrate and chronic supplementation of 13 mmol of nitrate over 8 days [24]. These subjects improved their results by 5.8 and 5.0 s through acute intake and by 7.0 and 0.5 s through chronic intake. Additionally, in a submaximal incremental test among trained triathletes, an improvement in distance and maintenance was observed (albeit without obtaining significant results), meaning that “the need to individually analyze the positive or negative response to this supplementation in the case of athletes trained may be the best option” [30].

5. Conclusions

The variety of results makes it difficult to draw clear conclusions about nitrate supplementation among endurance cyclic sports performance athletes. Although most of the studies showed no significant differences in the performance of the endurance-trained athletes after nitrate supplementation, some studies indicated that with the same maximal and mean power, the VO2 values decrease after nitrate supplementation. Furthermore, the time to exhaustion was delayed with nitrate supplementation in a race time trial between 5 and 30 min. Both results suggest enhanced performance.
The dose necessary for a significant effect remains unclear since some results used acute doses of 12 mmol of nitrate and others applied smaller doses (up to 6 mmol/day of nitrate). Finally, the best period for ingestion is 2–3 h before competing. Further investigations are required to confirm the findings of the present study.

Author Contributions

J.L.C.: conceptualization, conceived and designed the investigation, analyzed and interpreted the data, drafted the paper, and approved the final version submitted for publication. F.A.-C.: investigation, analyzed and interpreted the data, and writing. H.P.-G.: critically reviewed the paper, approved the final version submitted for publication, and funding acquisition. S.L.J.: critically reviewed the paper, interpreted the data, writing, and funding acquisition. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Acknowledgments

The authors declare no funding sources.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Jones, A.M. Dietary nitrate supplementation and exercise performance. Sports Med. 2014, 44 (Suppl. 1), 35–45. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Bescos, R.; Sureda, A.; Tur, J.A.; Pons, A. The effect of nitric-oxide-related supplements on human performance. Sports Med. 2012, 42, 99–117. [Google Scholar] [CrossRef] [PubMed]
  3. Flueck, J.L.; Bogdanova, A.; Mettler, S.; Perret, C. Is beetroot juice more effective than sodium nitrate? The effects of equimolar nitrate dosages of nitrate-rich beetroot juice and sodium nitrate on oxygen consumption during exercise. Appl. Physiol. Nutr. Metab. 2016, 41, 421–429. [Google Scholar] [CrossRef]
  4. Hord, N.G.; Tang, Y.; Bryan, N.S. Food sources of nitrates and nitrites: The physiologic context for potential health benefits. Am. J. Clin. Nutr. 2009, 90, 1–10. [Google Scholar] [CrossRef] [PubMed]
  5. Campos, H.O.; Drummond, L.R.; Rodrigues, Q.T.; Machado, F.S.M.; Pires, W.; Wanner, S.P.; Coimbra, C.C. Nitrate supplementation improves physical performance specifically in non-athletes during prolonged open-ended tests: A systematic review and meta-analysis. Br. J. Nutr. 2018, 119, 636–657. [Google Scholar] [CrossRef]
  6. Bailey, S.J.; Fulford, J.; Vanhatalo, A.; Winyard, P.G.; Blackwell, J.R.; DiMenna, F.J.; Wilkerson, D.P.; Benjamin, N.; Jones, A.M. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J. Appl. Physiol. (1985) 2010, 109, 135–148. [Google Scholar] [CrossRef] [Green Version]
  7. Bailey, S.J.; Varnham, R.L.; DiMenna, F.J.; Breese, B.C.; Wylie, L.J.; Jones, A.M. Inorganic nitrate supplementation improves muscle oxygenation, O2 uptake kinetics, and exercise tolerance at high but not low pedal rates. J. Appl. Physiol. (1985) 2015, 118, 1396–1405. [Google Scholar] [CrossRef]
  8. Dominguez, R.; Cuenca, E.; Mate-Munoz, J.L.; Garcia-Fernandez, P.; Serra-Paya, N.; Estevan, M.C.; Herreros, P.V.; Garnacho-Castano, M.V. Effects of Beetroot Juice Supplementation on Cardiorespiratory Endurance in Athletes. A Systematic Review. Nutrients 2017, 9, 43. [Google Scholar] [CrossRef] [Green Version]
  9. McMahon, N.F.; Leveritt, M.D.; Pavey, T.G. The Effect of Dietary Nitrate Supplementation on Endurance Exercise Performance in Healthy Adults: A Systematic Review and Meta-Analysis. Sports Med. 2017, 47, 735–756. [Google Scholar] [CrossRef] [Green Version]
  10. Vanhatalo, A.; Bailey, S.J.; Blackwell, J.R.; DiMenna, F.J.; Pavey, T.G.; Wilkerson, D.P.; Benjamin, N.; Winyard, P.G.; Jones, A.M. Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2010, 299, 1121–1131. [Google Scholar] [CrossRef] [Green Version]
  11. Aucouturier, J.; Boissiere, J.; Pawlak-Chaouch, M.; Cuvelier, G.; Gamelin, F.X. Effect of dietary nitrate supplementation on tolerance to supramaximal intensity intermittent exercise. Nitric Oxide 2015, 49, 16–25. [Google Scholar] [CrossRef] [PubMed]
  12. Jones, A.M. Influence of dietary nitrate on the physiological determinants of exercise performance: A critical review. Appl. Physiol. Nutr. Metab. 2014, 39, 1019–1028. [Google Scholar] [CrossRef] [PubMed]
  13. Pawlak-Chaouch, M.; Boissiere, J.; Munyaneza, D.; Gamelin, F.X.; Cuvelier, G.; Berthoin, S.; Aucouturier, J. Beetroot Juice Does Not Enhance Supramaximal Intermittent Exercise Performance in Elite Endurance Athletes. J. Am. Coll. Nutr. 2019, 38, 729–738. [Google Scholar] [CrossRef] [PubMed]
  14. Bailey, S.J.; Winyard, P.; Vanhatalo, A.; Blackwell, J.R.; Dimenna, F.J.; Wilkerson, D.P.; Tarr, J.; Benjamin, N.; Jones, A.M. Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J. Appl. Physiol. (1985) 2009, 107, 1144–1155. [Google Scholar] [CrossRef] [Green Version]
  15. Vasconcellos, J.; Henrique Silvestre, D.; Dos Santos Baiao, D.; Werneck-de-Castro, J.P.; Silveira Alvares, T.; Paschoalin, V.M. A Single Dose of Beetroot Gel Rich in Nitrate Does Not Improve Performance but Lowers Blood Glucose in Physically Active Individuals. J. Nutr. Metab. 2017, 2017, 7853034. [Google Scholar] [CrossRef]
  16. Kelly, J.; Vanhatalo, A.; Wilkerson, D.P.; Wylie, L.J.; Jones, A.M. Effects of nitrate on the power-duration relationship for severe-intensity exercise. Med. Sci. Sports Exerc. 2013, 45, 1798–1806. [Google Scholar] [CrossRef] [Green Version]
  17. Haider, G.; Folland, J.P. Nitrate supplementation enhances the contractile properties of human skeletal muscle. Med. Sci. Sports Exerc. 2014, 46, 2234–2243. [Google Scholar] [CrossRef]
  18. Pollock, A.; Berge, E. How to do a systematic review. Int. J. Stroke 2018, 13, 138–156. [Google Scholar] [CrossRef]
  19. Moher, D.; Liberati, A.; Tetzlaff, J.; Altman, D.G.; Group, P. Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement. PLoS Med. 2009, 6, e1000097. [Google Scholar] [CrossRef] [Green Version]
  20. Law, M.; Stewart, D.; Pollock, N.; Letts, L.; Bosch, J.; Westmorland, M. Guidelines for Critical Review Form—Quantitative Studies 1998; McMaster University: Hamilton, ON, Canada, 2008. [Google Scholar]
  21. Balsalobre-Fernandez, C.; Romero-Moraleda, B.; Cupeiro, R.; Peinado, A.B.; Butragueno, J.; Benito, P.J. The effects of beetroot juice supplementation on exercise economy, rating of perceived exertion and running mechanics in elite distance runners: A double-blinded, randomized study. PLoS ONE 2018, 13, e0200517. [Google Scholar] [CrossRef]
  22. Bescos, R.; Ferrer-Roca, V.; Galilea, P.A.; Roig, A.; Drobnic, F.; Sureda, A.; Martorell, M.; Cordova, A.; Tur, J.A.; Pons, A. Sodium nitrate supplementation does not enhance performance of endurance athletes. Med. Sci. Sports Exerc. 2012, 44, 2400–2409. [Google Scholar] [CrossRef] [PubMed]
  23. Bescos, R.; Rodriguez, F.A.; Iglesias, X.; Ferrer, M.D.; Iborra, E.; Pons, A. Acute administration of inorganic nitrate reduces VO(2peak) in endurance athletes. Med. Sci. Sports Exerc. 2011, 43, 1979–1986. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Boorsma, R.K.; Whitfield, J.; Spriet, L.L. Beetroot juice supplementation does not improve performance of elite 1500-m runners. Med. Sci. Sports Exerc. 2014, 46, 2326–2334. [Google Scholar] [CrossRef] [PubMed]
  25. Callahan, M.J.; Parr, E.B.; Hawley, J.A.; Burke, L.M. Single and Combined Effects of Beetroot Crystals and Sodium Bicarbonate on 4-km Cycling Time Trial Performance. Int. J. Sport Nutr. Exerc. Metab. 2017, 27, 271–278. [Google Scholar] [CrossRef] [PubMed]
  26. Cermak, N.M.; Gibala, M.J.; van Loon, L.J. Nitrate supplementation’s improvement of 10-km time-trial performance in trained cyclists. Int. J. Sport Nutr. Exerc. Metab. 2012, 22, 64–71. [Google Scholar] [CrossRef]
  27. Cermak, N.M.; Res, P.; Stinkens, R.; Lundberg, J.O.; Gibala, M.J.; van Loon, L.J. No improvement in endurance performance after a single dose of beetroot juice. Int. J. Sport Nutr. Exerc. Metab. 2012, 22, 470–478. [Google Scholar] [CrossRef] [Green Version]
  28. Christensen, P.M.; Nyberg, M.; Bangsbo, J. Influence of nitrate supplementation on VO2 kinetics and endurance of elite cyclists. Scand. J. Med. Sci. Sports 2013, 23, 21–31. [Google Scholar] [CrossRef]
  29. Esen, O.; Nicholas, C.; Morris, M.; Bailey, S.J. No Effect of Beetroot Juice Supplementation on 100-m and 200-m Swimming Performance in Moderately Trained Swimmers. Int. J. Sports Physiol. Perform. 2019, 14, 706–710. [Google Scholar] [CrossRef]
  30. Garnacho-Castano, M.V.; Palau-Salva, G.; Cuenca, E.; Munoz-Gonzalez, A.; Garcia-Fernandez, P.; Del Carmen Lozano-Estevan, M.; Veiga-Herreros, P.; Mate-Munoz, J.L.; Dominguez, R. Effects of a single dose of beetroot juice on cycling time trial performance at ventilatory thresholds intensity in male triathletes. J. Int. Soc. Sports Nutr. 2018, 15, 49. [Google Scholar] [CrossRef] [Green Version]
  31. Glaister, M.; Pattison, J.R.; Muniz-Pumares, D.; Patterson, S.D.; Foley, P. Effects of dietary nitrate, caffeine, and their combination on 20-km cycling time trial performance. J. Strength Cond. Res. 2015, 29, 165–174. [Google Scholar] [CrossRef] [Green Version]
  32. Hoon, M.W.; Hopkins, W.G.; Jones, A.M.; Martin, D.T.; Halson, S.L.; West, N.P.; Johnson, N.A.; Burke, L.M. Nitrate supplementation and high-intensity performance in competitive cyclists. Appl. Physiol. Nutr. Metab. 2014, 39, 1043–1049. [Google Scholar] [CrossRef] [PubMed]
  33. Hoon, M.W.; Jones, A.M.; Johnson, N.A.; Blackwell, J.R.; Broad, E.M.; Lundy, B.; Rice, A.J.; Burke, L.M. The effect of variable doses of inorganic nitrate-rich beetroot juice on simulated 2000-m rowing performance in trained athletes. Int. J. Sports Physiol. Perform. 2014, 9, 615–620. [Google Scholar] [CrossRef] [PubMed]
  34. Lane, S.C.; Hawley, J.A.; Desbrow, B.; Jones, A.M.; Blackwell, J.R.; Ross, M.L.; Zemski, A.J.; Burke, L.M. Single and combined effects of beetroot juice and caffeine supplementation on cycling time trial performance. Appl. Physiol. Nutr. Metab. 2014, 39, 1050–1057. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Lansley, K.E.; Winyard, P.G.; Bailey, S.J.; Vanhatalo, A.; Wilkerson, D.P.; Blackwell, J.R.; Gilchrist, M.; Benjamin, N.; Jones, A.M. Acute dietary nitrate supplementation improves cycling time trial performance. Med. Sci. Sports Exerc. 2011, 43, 1125–1131. [Google Scholar] [CrossRef] [Green Version]
  36. Lowings, S.; Shannon, O.M.; Deighton, K.; Matu, J.; Barlow, M.J. Effect of Dietary Nitrate Supplementation on Swimming Performance in Trained Swimmers. Int. J. Sport Nutr. Exerc. Metab. 2017, 27, 377–384. [Google Scholar] [CrossRef]
  37. MacLeod, K.E.; Nugent, S.F.; Barr, S.I.; Koehle, M.S.; Sporer, B.C.; MacInnis, M.J. Acute Beetroot Juice Supplementation Does Not Improve Cycling Performance in Normoxia or Moderate Hypoxia. Int. J. Sport Nutr. Exerc. Metab. 2015, 25, 359–366. [Google Scholar] [CrossRef]
  38. McQuillan, J.A.; Dulson, D.K.; Laursen, P.B.; Kilding, A.E. Dietary Nitrate Fails to Improve 1 and 4 km Cycling Performance in Highly Trained Cyclists. Int. J. Sport. Nutr. Exerc. Metab. 2017, 27, 255–263. [Google Scholar] [CrossRef]
  39. McQuillan, J.A.; Dulson, D.K.; Laursen, P.B.; Kilding, A.E. The Effect of Dietary Nitrate Supplementation on Physiology and Performance in Trained Cyclists. Int. J. Sports Physiol. Perform. 2017, 12, 684–689. [Google Scholar] [CrossRef] [Green Version]
  40. Nyakayiru, J.M.; Jonvik, K.L.; Pinckaers, P.J.; Senden, J.; van Loon, L.J.; Verdijk, L.B. No Effect of Acute and 6-Day Nitrate Supplementation on VO2 and Time-Trial Performance in Highly Trained Cyclists. Int. J. Sport Nutr. Exerc. Metab. 2017, 27, 11–17. [Google Scholar] [CrossRef]
  41. Nyback, L.; Glannerud, C.; Larsson, G.; Weitzberg, E.; Shannon, O.M.; McGawley, K. Physiological and performance effects of nitrate supplementation during roller-skiing in normoxia and normobaric hypoxia. Nitric Oxide 2017, 70, 1–8. [Google Scholar] [CrossRef]
  42. Peacock, O.; Tjonna, A.E.; James, P.; Wisloff, U.; Welde, B.; Bohlke, N.; Smith, A.; Stokes, K.; Cook, C.; Sandbakk, O. Dietary nitrate does not enhance running performance in elite cross-country skiers. Med. Sci. Sports Exerc. 2012, 44, 2213–2219. [Google Scholar] [CrossRef] [PubMed]
  43. Pinna, M.; Roberto, S.; Milia, R.; Marongiu, E.; Olla, S.; Loi, A.; Migliaccio, G.M.; Padulo, J.; Orlandi, C.; Tocco, F.; et al. Effect of beetroot juice supplementation on aerobic response during swimming. Nutrients 2014, 6, 605–615. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Rokkedal-Lausch, T.; Franch, J.; Poulsen, M.K.; Thomsen, L.P.; Weitzberg, E.; Kamavuako, E.N.; Karbing, D.S.; Larsen, R.G. Chronic high-dose beetroot juice supplementation improves time trial performance of well-trained cyclists in normoxia and hypoxia. Nitric Oxide 2019, 85, 44–52. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Shannon, O.M.; Barlow, M.J.; Duckworth, L.; Williams, E.; Wort, G.; Woods, D.; Siervo, M.; O’Hara, J.P. Dietary nitrate supplementation enhances short but not longer duration running time-trial performance. Eur. J. Appl. Physiol. 2017, 117, 775–785. [Google Scholar] [CrossRef] [PubMed]
  46. Wilkerson, D.P.; Hayward, G.M.; Bailey, S.J.; Vanhatalo, A.; Blackwell, J.R.; Jones, A.M. Influence of acute dietary nitrate supplementation on 50 mile time trial performance in well-trained cyclists. Eur. J. Appl. Physiol. 2012, 112, 4127–4134. [Google Scholar] [CrossRef] [PubMed]
  47. Jeukendrup, A.E.; Craig, N.P.; Hawley, J.A. The bioenergetics of World Class Cycling. J. Sci. Med. Sport 2000, 3, 414–433. [Google Scholar] [CrossRef]
  48. De Pauw, K.; Roelands, B.; Cheung, S.S.; de Geus, B.; Rietjens, G.; Meeusen, R. Guidelines to classify subject groups in sport-science research. Int. J. Sports Physiol. Perform. 2013, 8, 111–122. [Google Scholar] [CrossRef] [Green Version]
  49. Govoni, M.; Jansson, E.A.; Weitzberg, E.; Lundberg, J.O. The increase in plasma nitrite after a dietary nitrate load is markedly attenuated by an antibacterial mouthwash. Nitric Oxide 2008, 19, 333–337. [Google Scholar] [CrossRef]
  50. Lansley, K.E.; Winyard, P.G.; Fulford, J.; Vanhatalo, A.; Bailey, S.J.; Blackwell, J.R.; DiMenna, F.J.; Gilchrist, M.; Benjamin, N.; Jones, A.M. Dietary nitrate supplementation reduces the O2 cost of walking and running: A placebo-controlled study. J. Appl. Physiol. (1985) 2011, 110, 591–600. [Google Scholar] [CrossRef] [Green Version]
  51. Ormsbee, M.J.; Bach, C.W.; Baur, D.A. Pre-exercise nutrition: The role of macronutrients, modified starches and supplements on metabolism and endurance performance. Nutrients 2014, 6, 1782–1808. [Google Scholar] [CrossRef] [Green Version]
  52. Larsen, F.J.; Schiffer, T.A.; Borniquel, S.; Sahlin, K.; Ekblom, B.; Lundberg, J.O.; Weitzberg, E. Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 2011, 13, 149–159. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Porcelli, S.; Ramaglia, M.; Bellistri, G.; Pavei, G.; Pugliese, L.; Montorsi, M.; Rasica, L.; Marzorati, M. Aerobic Fitness Affects the Exercise Performance Responses to Nitrate Supplementation. Med. Sci. Sports Exerc. 2015, 47, 1643–1651. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Totzeck, M.; Hendgen-Cotta, U.B.; Rammos, C.; Frommke, L.M.; Knackstedt, C.; Predel, H.G.; Kelm, M.; Rassaf, T. Higher endogenous nitrite levels are associated with superior exercise capacity in highly trained athletes. Nitric Oxide 2012, 27, 75–81. [Google Scholar] [CrossRef] [PubMed]
  55. Jonvik, K.L.; Nyakayiru, J.; Van Dijk, J.W.; Maase, K.; Ballak, S.B.; Senden, J.M.G.; Van Loon, L.J.C.; Verdijk, L.B. Repeated-sprint performance and plasma responses following beetroot juice supplementation do not differ between recreational, competitive and elite sprint athletes. Eur. J. Sport Sci. 2018, 18, 524–533. [Google Scholar] [CrossRef]
  56. Breese, B.C.; McNarry, M.A.; Marwood, S.; Blackwell, J.R.; Bailey, S.J.; Jones, A.M. Beetroot juice supplementation speeds O2 uptake kinetics and improves exercise tolerance during severe-intensity exercise initiated from an elevated metabolic rate. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2013, 305, 1441–1450. [Google Scholar] [CrossRef] [Green Version]
Nutrients 12 01796 g001 550
Figure 1. The main beneficial effects of nitric oxide on physical performance.
Figure 1. The main beneficial effects of nitric oxide on physical performance.
Nutrients 12 01796 g001
Nutrients 12 01796 g002 550
Figure 2. Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) flow. Summary of the systematic search and the study selection process.
Figure 2. Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) flow. Summary of the systematic search and the study selection process.
Nutrients 12 01796 g002
Table
Table 1. Classification of vegetables according to nitrate content *.
Table 1. Classification of vegetables according to nitrate content *.
Nitrate Content (mg/100 g Fresh Weight)Vegetable Varieties
Very low, <20Artichoke, asparagus, broad bean, eggplant, garlic, onion, green bean, mushroom, pea, pepper, potato, summer squash, sweet potato, tomato, watermelon
Low, 20 to <50Broccoli, carrot, cauliflower, cucumber, pumpkin, chicory
Middle, 50 to <100Cabbage, dill, turnip, savoy cabbage
High, 100 to <250Celeriac, Chinese cabbage, endive, fennel, kohlrabi, leek, parsley
Very high, >250Celery, cress, chervil, lettuce, red beetroot, spinach, rocket
* Information obtained from Hord et al. 2009 [4].
Table
Table 2. Methodological quality of the studies included in the systematic review.
Table 2. Methodological quality of the studies included in the systematic review.
Reference123456789101112131415Ts%MQ
Balsalobre et al. [22]11111111111111115100VG
Bescós et al. [18]1111111111111011493.3VG
Bescós et al. [23]1111111111111011493.3VG
Boorsma et al. [24]11111111111111115100VG
Callahan et al. [25]1111111111110011386.6VG
Cermak et al. [26]11111111111111115100VG
Cermak et al. [27]1111111111110011386.6VG
Christense et al. [28]11111111111111115100VG
Esen et al. [29]11111111111111115100VG
Garnacho et al. [30]11111111111111115100VG
Glaister et al. [31]11111111111111115100VG
Hoon et al. [32]11111111111111115100VG
Hoon et al. [33]11111111111111115100VG
Lane et al. [34]1111111111111011493.3VG
Lansley et al. [35]11111111111111115100VG
Lowings et al. [36]11111111111111115100VG
MacLeod et al. [37]11111111111111115100VG
McQuillan et al. [38]11111111111111115100VG
McQuillan et al. [39]11111111111111115100VG
Nyakayiru et al. [40]11111111111111115100VG
Nybäck et al. [41]11111111111111115100VG
Pawlak et al. [13]1110111101111111386.6VG
Peacock et al. [42]11111111111111115100VG
Pinna et al. [43]11111111111111115100VG
Rokkedal et al. [44]11111111111111115100VG
Shannon et al. [45]11111111111111115100VG
Wilkerson et al. [46]11111111111111115100VG
Ts—Total items fulfilled by study; 1—Criterion met; 0—Criterion not met; MQ—Methodological quality: P—poor ≤ 8 points, A—acceptable 9–10 points, G—good 11–12 points, VG—very good 13–15 points.
Table
Table 3. Summary of studies reviewed examining the effects of nitrate supplementation on trained athletes of cyclic endurance performance.
Table 3. Summary of studies reviewed examining the effects of nitrate supplementation on trained athletes of cyclic endurance performance.
ReferenceParticipantsAgeSports Experience and/or Training LoadVO2max (mL/kg/min)Supplementation ProtocolNitrate Dose (mmol/day)Last Dose (hours)Exercise ProtocolPrincipal Performance MeasuresResults
Balsalobre et al. [22]M, middle and long-distance runners (n = 12)26.3 ± 5.1International athletes with personal bests71.8 ± 5.2BJ 70 mL (15 days)6.524Incremental running test to exhaustionTime (s), RPE, SmO2 (%), VO2 (mL/kg/min)Time (S: 1269 ± 53.6 vs. PLA: 1230 ± 73.5, D = yes), RPE (S: 6 ± 1 vs. PLA: 7.7 ± 1, D = yes), SmO2 (S: 31 ± 6.9 vs. PLA: 27.7 ± 4.8, D = yes), VO2 (S: 69.5 ± 2.9 vs. PLA: 71.4 ± 4.8, D = no)
Bescós et al. [18]M, cyclists and triathletes (n = 13)32.6 ± 5.6Experience in endurance events: 8 ± 5 years,
training volume: 15.7 ± 5.0 h/wk		60 ± 7Sodium nitrate 250 mL (3 days)11.63Distance trial (40 min) in cycle ergometerDistance covered (km), Mean power output (W), VO2 (mL/kg/min)Distance covered (S: 26.4 ± 1.1 vs. PLA: 26.3 ± 1.2, D = no), mean power output (S: 258 ± 28 vs. PLA: 257 ± 28, D = no), VO2 (S: 51 ± 7.9 vs. PLA: 50.9 ± 6.6, D = no)
Bescós et al. [23]M, cyclists and triathletes (n = 11)34.3 ± 4.8Members of competitive squads65.1 ± 6.2Sodium nitrate 250 mL (1 day)11.83Submaximal cycling trial (4 × 6 min) (T1), incremental exercise test to exhaustion (T2)T1: VO2 (L/min) at 3.5 W/kg
T2: time (s), maximal power (W), VO2peak (L/min)		T1: VO2 (S: 0.5 ± 0.11 vs. 0.53 ± 0.06, D = no)
T2: time (S: 416 ± 32 vs. PLA: 409 ± 27, D = no), maximal power (S: 416 ± 29 vs. PLA: 410 ± 28, D = no), VO2peak (S: 4.64 ± 0.35 vs. PLA: 4.82 ± 0.33, D = yes)
Boorsma et al. [24]M, distance runners (n = 8)23.8 ± 5Elite distance;
experience: provincial, national, or international events;
training volume: 12.3 ± 4 h/wk		80 ± 5BJ 210 mL (1 day)19.51.5Submaximal treadmill run (T1), time trial 1500 m (T2)T1: VO2 (mL/min) T2: time (s)T1: VO2 (S: 4192 ± 113 vs. PLA: 4194 ± 90, D = no)
T2: time (S: 250 ± 7 vs. PLA: 250 ± 4, D = no)
BJ 210 mL (on the test day) and 140 (other days) (8 days)19.5 and 132.5Submaximal treadmill run (T1), time trial 1500 m (T2)T1: VO2 (mL/min) T2: time (s)T1: VO2 (S: 4299 ± 92 vs. PLA: 4216 ± 95, D = no)
T2: time (S: 250 ± 5 vs. PLA: 251 ± 4, D = no)
Callahan et al. [25]M, cyclists (n = 8)34 ± 7Well-trained cyclist65.2 ± 4.2Beetroot crystals 15 g (3 days)51Time trial 4 kmMean power output (W), time (s)Mean power output (S: 388 ± 54 vs. PLA: 386 ± 56, D = no), time (S: 337.4 ± 17.1 vs. PLA: 338.1 ± 18, D = no)
Cermak et al. [26]M, cyclists and triathletes (n = 12)31 ± 3Training history of ≈10 years,
training volume: ≈10 h/wk.		58 ± 2BJ 140 mL (6 days)82.5Submaximal cycling trail (2 × 30 min) (P1), time trial 10 km (T2)T1: VO2 (L/min)
T2: mean power output (W), Time (s)		T1: VO2 (S: 2.94 ± 0.1 vs. PLA: 3.1 ± 0.09, D = yes)
T2: mean power output (S: 294 ± 41.5 vs. PLA: 288 ± 41.5, D = yes), time (S: 953 ± 72.5 vs. PLA: 965 ± 72.5, D = yes)
Cermak et al. [27]M, cyclists and triathletes (n = 20)26 ± 1Training experience: >3 times/wk for several years60 ± 1BJ 140 mL (1 day)8.72.5Time trial ≈1 hMean power output (W), time (min)Mean power output (S: 275 ± 7 vs. PLA: 278 ± 7, D = no), time (S: 65.5 ± 1.1 vs. PLA: 65 ± 1.1, D = no)
Christensen et al. [28]M, cyclists (n = 10)29 ± 4Experience: elite cyclists competing at the highest domestic level72.1 ± 4.5BJ 500 mL (6 days)83Submaximal cycling trial at 70% Wmax (2 × 6 min) (T1), repeated sprint test (6 × 20 s) (T2), time trial 400 kcal (≈20 min) (T3)T1: VO2 (mL/min)
T2: mean power output (W)
T3: mean power output (W), time (min)		P1: VO2 (S: 792 ± 82 vs. PLA: 806 ± 82, D = no)
P2: mean power output (S: 630 ± 84 vs. PLA: 630 ± 92, D = no)
P3: mean power output (S: 290 ± 43 vs. PLA: 285 ± 44, D = no), time (S: 18.20 vs. PLA: 18.37, D = no)
Esen et al. [29]M (n = 5) and F (n = 5), swimmers (n = 10)22 ± 6Experience: ≥10 years competing at club standard and ≥5 years competing in regional and university-level.
Training volume: 3–4 times/wk and 6–8 h/wk		2212BJ 140 mL (3 days)83Time trial 200 m (T1),
time trial 100 m (T2)		T1: time (s)
T2: time (s)		T1: time (S: 152.6 ± 14.1 vs. PLA: 152.5 ± 14.1, D = no)
T2: time (S: 69.5 ± 7.2 vs. PLA: 69.4 ± 7.4, D = no)
Garnacho et al. [30]M, triathletes (n = 12)39.3 ± 7.5Experience: National (n = 8) and international (n = 4) level of competition; training volume: ≥4 times/wk with ≥1 h/session 54.8 ± 3.1BJ 70 mL (1 day)6.53Time trial at VT1 (30 min) and VT2 (15 min)VO2 (L/min), VCO2 (L/min), time (s) at VT2VO2 (S: 73.8 ± 8.9 vs. PLA: 72.2 ± 9.4, D = no), VCO2 (S: 3.1 ± 0.3 vs. PLA: 2.9 ± 0.4, D = yes), time VT2 (S: 933 vs. PLA: 882, D = no)
Glaister et al. [31]F, cyclists and triathletes (n = 14)31 ± 7Experience: ≈13 years actively in sport; training volume: 10.7 ± 2.2 h/wk at the time of the investigation52.3 ± 4.9BJ 70 mL (1 day)7.32.5Time trial 20 kmTime (min)Time (S: 35.33 ± 1.5 vs. PLA: 35.37 ± 1.7, D = no)
Hoon et al. [32]
M, cyclists (n = 26)20.3 ± 1.4Trained male cyclists that were involved in a 6 week training camp at the Australian Institute of Sport-BJ 70 mL (1 day)4.11.25Time trail 4 min ×2Mean power output (W)Mean power output (S: 403 ± 52 vs. PLA: 396 ± 57, D = no)
2.5Time trail 4 min ×2Mean power output (W)Mean power output (S: 402 ± 47 vs. PLA: 396 ± 57, D = no)
Hoon et al. [33]M, rowers (n = 10)20.6 ± 2.5Highly trained; 2000-m personal-best time: 6 min, 17 s ± 10 s; training volume: 16.9 ± 3.4 h/wk-BJ 70 mL (1 day)4.22Time trial 2000 mTime (s)Time (S: 383.4 ± 8.7 vs. PLA: 3835 ± 9, D = no)
BJ 140 mL (1 day)8.42Time trial 2000 mTime (s)Time (S: 381.9 ± 9 vs. PLA: 383.5 ± 9, D = yes)
Lane et al. [34]M (n = 12) and F (n = 12), cyclists and triathletes (n = 24)31 ± 7Competitive level71.6 ± 4.6BJ 140 mL (1 day)8.42Time trial 43.83 kmTime (min), power output (W)Time (S: 64 ± 2.8 vs. PLA: 63.5 ± 3.2, D = no), power output (S:298 ± 35 vs. PLA: 303 ± 41, D = no)
28 ± 6Competitive level59.9 ± 5.1BJ 140 mL (1 day)8.42Time trial 29.35 kmTime (min), power output (W)Time (p > 0.05), power output (S: 207 ± 31 vs. PLA: 207 ± 29, D = no)
Lansley et al. [35]M, cyclists (n = 9)21 ± 4Competitive level56 ± 5.7BJ 500 mL (1 day)6.22.5Time trial 4 kmTime (min), mean power output (W), VO2 (L/min)Time (S: 6.27 ± 0.35 vs. PLA: 6.45 ± 0.42, D = yes), mean power output (S: 292 ± 44 vs. PLA: 279 ± 51, D = yes), VO2 (S: 4.46 ± 0.5 vs. PLA: 4.36 ± 0.47, D = no)
Time trial 16.1 kmTime (min), mean power output (W), VO2 (L/min)Time (S: 26.9 ± 1.8 vs. PLA: 27.7 ± 2.1, D = yes), mean power output (S: 247 ± 44 vs. PLA: 233 ± 43, D = yes), VO2 (S: 4.32 ± 0.47 vs. PLA: 4.19 ± 0.56, D = no)
Lowings et al. [36]M (n = 5) and F (n = 5), swimmers (n = 10)20 ± 1Competitive level; training volume: ≥3 times/wk−BJ 140 mL (1 day)12.53Time trial 168 mTime (s)Time (p = 0.144), nitric oxide bioavailability +
MacLeod et al. [37]M, cyclists (n = 11)29.3 ± 5.1Trained cyclists that met the inclusion criterion VO2peak > 5 L/min67.5 ± 5.8BJ 70 mL (1 day)6.52Time trial 15 kmTime (s), mean power output (W/kg)Time (S: 961 ± 54 vs. PLA: 954 ± 47, D = no), mean power output (S: 3.7 ± 0.4 vs. PLA: 3.8 ± 0.3, D = no)
McQuillan et al. [38]M, cyclists (n = 9)27 ± 9Endurance trained cyclists and competing in cycle races in the 3 months preceding the study; training volume: regular 11.4 ± 2.6 hr/wk and 310.27 km/wk68 ± 3BJ 140 mL (3 days)82.5Time trial 4 kmTime (s), mean power output (W)Time (S: 341 ± 12 vs. PLA: 340 ± 10, D = no), mean power output (S: 390 ± 45 vs. PLA: 393 ± 37, D = no)
BJ 140 mL (4 days)82.5Time trial 1 kmTime (s), mean power output (W)Time (S: 79.6 ± 3.5 vs. PLA: 79.2 ± 2.9, D = no), mean power output (S: 8495 ± 61 vs. PLA: 503 ± 51, D = no)
BJ 140 mL (6 days)82.5Time trial 4 kmTime (s), mean power output (W)Time (S: 340 ± 10 vs. PLA: 340 ± 11, D = no), mean power output (S: 394 ± 38 vs. PLA: 393 ± 37, D = no)
BJ 140 mL (7 days)82.5Time trial 1 kmTime (s), mean power output (W)Time (S: 79.3 ± 3.3 vs. PLA: 79 ± 3, D = no), mean power output (S: 501 ± 59 vs. PLA: 505 ± 52, D = no)
McQuillan et al. [39]M, cyclists (n = 8)26 ± 8Well-trained endurance cyclists;
training volume: weekly training duration 9 ± 3 h/wk		63 ± 4BJ 70 mL (8 days)42Time trial 4 kmTime (s), mean power output (W)Time (S: 343.6 ± 14.3 vs. PLA: 344.8 ± 14, D = no), mean power output (S: 380 ± 41 vs. PLA: 375 ± 40, D = no)
Nyakayiru et al. [40]M, cyclists and triathletes (n = 17)25 ± 4Competitive cyclist;
experience: 9.6 ± 5.1 years; training volume: 9.7 ± 3.7 h/wk		65 ± 4Sodium nitrate 1097 mg (1 day)12.94Submaximal cycling trial at 45% Wmax (30 min) and 65% Wmax (30 min) (T1), time trial 10km (T2),T1: VO2
T2: time (s)		T1: VO2 (D = no)
T2: time (S: 1022 ± 72 vs. PLA: 1017 ± 71, D = no)
Sodium nitrate 1097 mg (6 days)12.94submaximal cycling trial at 45% Wmax (30 min) and 65% Wmax (30min) (T1), time trial 10km (T2)T1: VO2
T2: time (s)		T1: VO2 (D = no)
T2: time (S: 1004 ± 61 vs. PLA: 1017 ± 71, D = no)
Nybäck et al. [41]M (n = 5) and F (n = 3), cross-country skiers (n = 8)21.8 ± 2.8Well-trained, competing at a national level71.5 ± 4.7 (M)BJ 140 mL (1 day)132.5Submaximal test (2 × 6 min) (T1), time trial 1 km (T2)T1: VO2 (L/min) T2: time (s)T1: VO2 (S: 2.92 ± 0.48 vs. PLA: 2.9 ± 0.49, D = no)
T2: time (S: 297 ± 29 vs. PLA: 295 ± 29, D = no)
Pawlak et al. [13]M, triathletes and runners (n = 9)21.7 ± 3.7Runners from clubs engaged in intense endurance exercise training and competition; inclusion based on VO2max > 65 mL/kg/min71.1 ± 5.2BJ 500 mL (3 days)5.43Repeated sprint test to exhaustionNo. of sprints completed, mean power output (W), VO2 (mL/min)No. of sprints completed (S: 13.9 ± 4 vs. PLA: 14.2 ± 4.5, D = no), mean power output (S: 579.2 ± 57.7 vs. PLA: 578.9 ± 54.3, D = no), VO2 (S: 3378.5 ± 681.8 vs. PLA: 3466.1 ± 505.3, D = no)
Peacock et al. [42]M, cross-country skiers (n = 10)18Experience: national and international standard junior skiers, all among the 20 best in the 2010 Norwegian Cup Series; training history: 502 ± 45 h/year69.6 ± 5.1Potassium nitrate
1 g (1 day)		9.92.5Submaximal test at 55% VO2max (5 min) and 75% VO2max (5 min) (T1), time trial 5 km (T2)T1: VO2 (L/min)
T2: time (s)		T1: VO2 (S: 3.77 ± 0.62 vs. PLA: 3.89 ± 0.39, D = no)
T2: time (S: 1005 ± 53 vs. PLA: 996 ± 49, D = no)
Pinna et al. [43]M, swimmers (n = 14)34.7 ± 7.5Master athletes involved in regional and national competitions; training volume: average of 6.5 ± 0.8 h per week; training frequency ranged 3–4 times/wk, with 3000–5000 m distance covered each time42.7 ± 2.6BJ 500 mL (6 days)5.53Incremental swimming testWorkload (kg/min), VO2 (mL/min)Workload (S: 6.7 ± 1.1 vs. PLA: 6.35 ± 1 D = yes), VO2 (S: 2741 ± 454 vs. PLA: 2817 ± 545, D = no)
Rokkedal et al. [44]M, cyclists (n = 12)29.1 ± 7.7Well-trained in performance level 4 [47,48]66.4 ± 5.3BJ 140 mL (7 days)12.42.75Time trial 10 kmPower output (W), time (s), VO2 (mL/min)Power output (S: 315.8 ± 13.2 vs. PLA: 311.3 ± 13.2, D = yes), time (S: 884,5 ± 16 vs. PLA: 890,1 ± 16, D = yes), VO2 (D = yes)
Shannon et al. [45]M, runners and triathletes (n = 8)28.3 ± 5.8Experience in competing in running events62.3 ± 8.1BJ 140 mL (1 day)12.53Time trial 1500 mTime (s), VO2 (mL/kg/min)Time (S: 319.6 ± 36.2 vs. PLA: 325.7 ± 38.8, D = yes) VO2 (S: 53.4 ± 6.8 vs. PLA: 53.9 ± 6.9, D = no)
Time trial 10 kmTime (s), VO2 (mL/kg/min)Time (S: 2643.1 ± 324,1 vs. PLA: 2649.9 ± 319.8, D = no), VO2 (S: 49 ± 6 vs. PLA: 48.6 ± 6.3, D = no)
Wilkerson et al. [46]M, cyclists (n = 8)31 ± 11Well-trained subjects;
training volume: 5.8 ± 1.0 times per week and completed 11.1 ± 2.5 h training per week		63 ± 8BJ 500 mL (1 day)6.22.5Time trial 50 milesTime (min), mean power output (W)Time (S: 136.7 ± 5.6 vs. PLA: 137.9 ± 6.4, D = no), mean power output (S: 238 ± 22 vs. PLA: 235 ± 27, D = no)
M: male, F: female, VO2max: maximal oxygen uptake, VO2peak: peak oxygen uptake, BJ: beetroot juice, SmO2: vastus lateralis oxygen saturation, RPE: rating of perceived exertion, VCO2: expired carbon dioxide, D: statistical difference, T: trial, VT: ventilatory threshold, PLA: placebo, S: supplemented.
Table
Table 4. Summary table with studies on the cyclic endurance performance of trained athletes.
Table 4. Summary table with studies on the cyclic endurance performance of trained athletes.
SportnSexNumber of StudiesNumber of Significant StudiesResults on Respiratory and/or Performance Parameters
PerformanceRespiratory
Cycling101All M92 [35,44]Time [35,44], MPO [35,44]VO2max [44]
Cycling and Triathlon9785 M, 12 F62 [23,26]Time [26], MPO [26]VO2peak [23]
VO2max [26]
Athletics and Triathlon17All M21 [22]Time, RPESmO2
Triathlon2612 M, 14 F 2
Athletics20All M21 [45]Time
Cross-country skiing1815 M, 3 F2
Rowing10All M 11 [33]Time
Swimming3424 M, 10 F31 [43]TimeReduction of aerobic energy cost
Six different sports323Only one study exclusively with F [31] and four mixed studies [29,34,36,41]27875
M: male, F: female, VO2max: maximal oxygen uptake, VO2peak: peak oxygen uptake, SmO2: vastus lateralis oxygen saturation, RPE: rating of perceived exertion, MPO: mean power output.

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MDPI and ACS Style

Lorenzo Calvo, J.; Alorda-Capo, F.; Pareja-Galeano, H.; Jiménez, S.L. Influence of Nitrate Supplementation on Endurance Cyclic Sports Performance: A Systematic Review. Nutrients 2020, 12, 1796. https://doi.org/10.3390/nu12061796

AMA Style

Lorenzo Calvo J, Alorda-Capo F, Pareja-Galeano H, Jiménez SL. Influence of Nitrate Supplementation on Endurance Cyclic Sports Performance: A Systematic Review. Nutrients. 2020; 12(6):1796. https://doi.org/10.3390/nu12061796

Chicago/Turabian Style

Lorenzo Calvo, Jorge, Francesca Alorda-Capo, Helios Pareja-Galeano, and Sergio L. Jiménez. 2020. "Influence of Nitrate Supplementation on Endurance Cyclic Sports Performance: A Systematic Review" Nutrients 12, no. 6: 1796. https://doi.org/10.3390/nu12061796

APA Style

Lorenzo Calvo, J., Alorda-Capo, F., Pareja-Galeano, H., & Jiménez, S. L. (2020). Influence of Nitrate Supplementation on Endurance Cyclic Sports Performance: A Systematic Review. Nutrients, 12(6), 1796. https://doi.org/10.3390/nu12061796

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