Next Article in Journal
Vitamin E Levels in Preterm and Full-Term Infants: A Systematic Review
Next Article in Special Issue
Preventive Effects of Ginkgo-Extract EGb 761® on Noise Trauma-Induced Cochlear Synaptopathy
Previous Article in Journal
The Association of Formula Protein Content and Growth in Early Infancy: A Systematic Review and Meta-Analysis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Nutrients
Volume 14
Issue 11
10.3390/nu14112256
nutrients-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Vascular–Metabolic Risk Factors and Psychological Stress in Patients with Chronic Tinnitus

by
Benjamin Boecking
1,
Sven Klasing
1,
Michael Walter
2,
Petra Brueggemann
1,
Amarjargal Nyamaa
1,
Matthias Rose
3 and
Birgit Mazurek
1,*
1
Tinnitus Center, Charité, Universitätsmedizin Berlin, 10117 Berlin, Germany
2
Institute of Clinical Chemistry and Laboratory Medicine, Universitätsmedizin Rostock, 18057 Rostock, Germany
3
Medical Department, Division of Psychosomatic Medicine, Charité, Universitätsmedizin Berlin, 10117 Berlin, Germany
*
Author to whom correspondence should be addressed.
Nutrients 2022, 14(11), 2256; https://doi.org/10.3390/nu14112256
Submission received: 1 April 2022 / Revised: 12 May 2022 / Accepted: 17 May 2022 / Published: 28 May 2022
(This article belongs to the Special Issue Hearing Loss and Tinnitus—Impact of Different Factors concerning Vulnerability-Lifestyle, Nutrition, Epidemiological Factors and Gender)
Versions Notes

Abstract

:
Little is known about molecular correlates of chronic tinnitus. We examined interrelationships between vascular–metabolic risk factors, perceived stress, and other routine blood values in patients with chronic tinnitus. Two-hundred patients (51% female) were screened for 49 blood parameters pertaining to vascular–metabolic risk, immune function, and redox processes. They further completed perceived stress- and tinnitus-related distress questionnaires. Following descriptive analyses, gender-specific sets of age- and tinnitus-severity-adjusted regression models investigated associations between perceived stress and blood parameters. Patients reported mildly elevated levels of perceived stress. Elevated levels of total cholesterol (65% and 61% of female and male patients, respectively), non-HDL-c (43/50%), LDL-c (56/59%), and lipoprotein_a (28/14%) were accompanied by high rates of overweight (99/100%) and smoking (28/31%). A low-level inflammatory state was accompanied by reduced reactive oxygen species (ROS)-neutralizing capacity (reduced co-enzyme Q10 and SOD1 levels). Most vascular risk factors were not correlated with perceived stress, except for fibrinogen (ß = −0.34) as well as C-reactive protein (ß = −0.31, p < 0.05) in men, and MCV (ß = −0.26, p < 0.05) in women. Interrelations between blood parameters and stress levels need to be investigated within psychobehavioural frameworks across varying distress levels. Alongside psychological interventions, a low-level inflammatory state may be a route for pharmacological therapeutics.

1. Introduction

Chronic tinnitus—a symptom of interrelated biopsychological contributions—denotes the conscious awareness of a tonal or composite noise without identifiable corresponding external acoustic source. Prevalence estimates vary widely and range from 5 to 43% [1,2]. Whilst the majority of people habituate to the symptom [3], a proportion of patients experience psychological distress, which appears to facilitate symptom chronification [4]—possibly through interactions of pre-existing psychological vulnerability and cognitive–affective reaction patterns following symptom onset [5,6,7].
Biomarker research for chronic tinnitus is still in its infancy [8,9,10,11,12], yet a role of vascular risk factors and inflammatory processes has been suggested [13,14]. Other studies investigated cytokine changes [15], mean platelet volumes (MPV), and neutrophil-to-lymphocyte ratios (NLR) with inconclusive results. Some studies reported increased MPV [16,17] or NLRs [17,18] in tinnitus patients, whereas other studies did not find any such differences [19] or reported lower MPV levels in tinnitus patients compared to controls [20]. A few studies further reported an association between zinc status and tinnitus severity [21,22].
‘Stress’ has been defined as “the quality of experience […] which, through either overarousal or underarousal, results in psychological or physiological distress” [23]. Tinnitus-correlated psychological distress has been described using a variety of terms, including tinnitus handicap [24], tinnitus severity [25], tinnitus-related distress [26], tinnitus disability [27], tinnitus annoyance [28], tinnitus bother [29], or tinnitus distress [30]. Additionally, “perceived stress”, i.e., the degree to which people appraise situations as stressful [31] considerably overlaps with tinnitus-related distress [5,15,32,33].
Emotional distress and tinnitus symptomatology are intricately connected: For instance, perceived stress can trigger sudden hearing loss and facilitate tinnitus onset or chronification [34,35,36]—likely against a backdrop of psychological vulnerability [37]. Moreover, some low-quality evidence suggests an etiological role of perceived stress in the development of some chronic pain conditions [38]—which may share pathophysiological variance with chronic tinnitus presentations [32,39,40]. Concomitant to the tinnitus-symptom, psychological distress may prevent habituation to the tinnitus sound—and thereby facilitate symptom chronification and maintain affective arousal [41]. Lastly, the tinnitus sound itself can act as a stressor—therein closing a vicious cycle between perceived stress and tinnitus exacerbation [35,42].
Regarding biological underpinnings of perceived stress, Juster et al. [40] proposed 26 putative biomarkers that included immune, vascular-metabolic and oxidative parameters that were also denoted as possible transdiagnostic markers across various psychological conditions [43,44,45]. In this vain, biomarker candidates that were identified in patients with chronic tinnitus, and that relate to cardiovascular, inflammatory, or immune-related processes [12], may or may not overlap with biomarkers of perceived stress.
In this work, we thus explored interrelationships between perceived stress, vascular-metabolic risk factors, and routine blood parameters in patients with chronic tinnitus. First, we describe the obtained blood parameters relative to their reference ranges in our patient sample. Second, we investigate associations between ‘outstanding’ biomarkers and patients’ perceived stress levels—considering potential gender differences [46,47].

2. Materials and Methods

2.1. Participants

The present study reports questionnaire and blood parameter data from n = 200 patients with chronic tinnitus (51% female; Mage = 54.68; SD = 8.44) who (a) self-referred to the Tinnitus Center at Charité Universitätsmedizin Berlin between April 2016 and August 2017; (b) suffered from chronic tinnitus (lasting for >3 months); (c) were 18 years of age or older; and (d) completed, amongst other measures, the German Tinnitus- and Perceived Stress Questionnaire. Exclusion criteria included an inability to consent due to severe psychiatric or physical limitations, as well as a participation in any other research study. Upon arrival at the Tinnitus Center, participants provided blood samples (obtained via 1 × 2 mL, 1 × 6 mL EDTA, 2 × 4.5 mL lithium heparin, 2 × 4.5 mL serum, and 1 × 2.7 mL citrate tubes), underwent audiological testing (the results of which are reported elsewhere), and completed the psychological questionnaires. Ethical approval was obtained from Charité Universitätsmedizin Berlin (No: EA1/115/15). All research was performed in accordance with the Declaration of Helsinki and informed consent was obtained from all participants.

2.2. Measures

2.2.1. Blood Index Values

The obtained blood samples were screened for a number of indices. The following (I) cellular immune response markers were obtained: leukocytes, lymphocytes (total), lymphocytes (%), monocytes (total), monocytes (%), neutrophils (total), neutrophils (%), immature granulocytes (total), immature granulocytes (%), eosinophils (total), eosinophils (%), basophils (total), and basophils (%). (II) Inflammatory response markers included cytokines (TNF-α, IL-6) and acute-phase Proteins (CRP, fibrinogen, ferritin, thrombocytes, MPV). (III) Hematological markers included hemoglobin, hematocrit, erythrocytes, MCV, RDW_CV, MCH, and MCHC. Measured (IV) (co-)enzymes comprised superoxide_dismutase_1, superoxide_dismutase_2, lipid_peroxidase, and ubiquinone (Q10). (V) Vascular-metabolic risk markers included total cholesterol, triglycerides, HDL-c, non-HDL-c, LDL-c, and lipoprotein_a. (VI) Liver function markers included albumin, GOT, GPT, and gamma_GT. (VII) Kidney function markers included GFR and creatinine. (VIII) Purine metabolism was indexed by uric acid. Lastly, (IX) vitamins, minerals, and trace elements included calcium, magnesium, zinc, selenium, and vitamin D3.

2.2.2. Perceived Stress

Perceived stress was measured using the Perceived Stress Questionnaire (PSQ; [48,49]). ‘Tension’ explores tense disquietude, exhaustion, and lack of relaxation. ‘Worries’ assesses anxious concern for the future, and feelings of desperation and frustration. ‘Joy’ assesses positive feelings of challenge, joy, energy, and security, and ‘Demands’ assesses perceived environmental demands, such as lack of time, pressure, and overload. The scale consists of 20 items that are rated on a 4-point scale (1 = almost never; 2 = sometimes; 3 = often; 4 = almost always). All indices are linearly transformed to range from 0 to 100 and summed up to a total score for which joy is recoded.

2.2.3. Tinnitus-Related Distress

The German version of the tinnitus questionnaire [50,51] assesses tinnitus-related psychological distress. It consists of 52 statements that are answered on a 3-point scale (0 = not true; 1 = partly true; 2 = true) across five subscales (cognitive and emotional burden, persistence of sound, hearing difficulties, sleep difficulties, and somatic complaints). Based on clinical and research considerations, we include only the total score in our analysis [52,53]. The total score includes 40 items, with two items being included twice, thus yielding a score from zero to 84. Biesinger et al. [54] suggested a cut-off of 46 points to distinguish high vs. low symptom burden, i.e., “decompensated” vs. “compensated” tinnitus. The scale’s test–retest reliability is good (total score: r = 0.94; [55]).

2.3. Statistical Analyses

Statistical analyses included descriptive and univariate regression analyses. All analyses were computed using IBM SPSS Statistics (v. 24). The significance level was set to α = 0.05.

2.4. Data Preparation

Following visual inspection of the data, “extreme outliers” (defined as featuring z-factor values of >3.29) were excluded for each blood parameter.

2.5. Descriptive Analyses

For the blood parameter data, patient values were categorized as ‘normal’, ‘increased’, or ‘decreased’ using gender-specific norm-reference values that were provided by two processing laboratories (Labor Berlin—Charité Vivantes GmbH, biovis Diagnostik MVZ GmbH). Next, frequency counts of women and men with ‘increased’ or ‘decreased’ values were computed for each blood parameter.

2.6. Univariate Regression Analyses

To examine the impact of perceived- but not tinnitus-related distress on the measured blood parameters, we investigated gender-separate univariate regression models, with PSQ scores as independent variables, blood parameters as dependent variables, and age as well as tinnitus-related distress as covariates. Due to the scarcity of blood-parameter research, expected small effect sizes, and the conservativeness of the Bonferroni correction [56,57], we conducted separate regression analyses—thereby tolerating increased type-I error rates. Thus, our findings necessitate replication.

3. Results

Table 1 features an overview of the sociodemographic information.

3.1. Descriptive Indices

Table 2 features descriptive results for the obtained psychological indices; Table 3 for vascular-metabolic risk factors, and Table 4 for vascular-metabolic blood parameters.
No gender differences emerged. Lifetime histories and last-year incidents of vascular events were negligible (lifetime: coronary heart disease (n = 5); stroke (n = 3); cardiac insufficiency (n = 8); last-year: coronary heart disease (n = 2); stroke (n = 2); cardiac insufficiency (n = 5)).
Table 5 features means, standard deviations and reference values for non-vascular-metabolic blood parameters.
High proportions of both female and male patients showed decreased levels of superoxide–dismutase 1, lipid-corrected ubiquinone (Q10), and GFR, as well as increased levels of total cholesterol, non-HDL-c, and LDL-c. High proportions of female patients showed increased levels of lipoprotein_a, whilst high proportions of male patients showed increased levels of monocytes and decreased levels of zinc.

3.2. Associations between Perceived Stress and Blood Parameters

Age- and tinnitus-related distress-adjusted univariate regression analyses revealed associations between perceived stress and oxidative stress markers predominantly in female—and inflammatory and immunological markers predominantly in male patients with chronic tinnitus.
Specifically, the observed findings included MCV (positive association (+) with perceived stress, i.e., PSQ-total) in women, and CRP (negative association (−) with PSQ-total), fibrinogen (−), selenium (+), GPT (−), and basophils (+) in men.
Investigating subdimensions of perceived stress, the red cell number and volume-increasing stress response values with a reduction in MCHC were confirmed for both women and men. PSQ-worries predicted ferritin (+), MCV (+), MCHC (−), hematocrit (+), magnesium (+), zinc (+), superoxide–dismutase 2 (+), and lipoprotein_a (+) in women; and fibrinogen (−), MCV (+), MCHC (−), basophils (+), leukocytes (−), and neutrophils (−) in men. PSQ-tension predicted ferritin (+), MCV (+), hematocrit (+), hemoglobin (+), RDW-CV (−), zinc (+), and superoxide–dismutase 2 (+) in women; and fibrinogen (−), uric acid (−), MCHC (−), lipid-corrected ubiquinone (Q10) (−), and basophils (+) in men. PSQ-joy predicted ferritin (+), MCV (+), hematocrit (+), hemoglobin (+), RDW-CV (−), and zinc (+) in women; and MCV (+), MCHC (−), and IL-6 (+) in men. Finally, PSQ-demands predicted ferritin (+), MCV (+), MCHC (−), hematocrit (+), hemoglobin (+), superoxide–dismutase 2 (+), selenium (+), magnesium (+), and zinc (+) in women; and ferritin (−), uric acid (−), lipid-corrected ubiquinone (Q10) (−), and basophils (+) in men.
See Table 6 for an overview. Supplementary Figure S1 provides a visual conspectus.

4. Discussion

The present study sought to investigate (1) vascular-metabolic risk factors and blood parameters in patients with chronic tinnitus and (b) their associations with perceived stress.

4.1. Vascular–Metabolic Risk Factors

Substantial proportions of patients showed elevated levels of metabolic–vascular risk factors, including total cholesterol (women/men) (64.7/61.2%), non-HDL-c (43.1/50.0%), and LDL-c (55.9/59.2%), frequently within a context of overweight (BMI ≥ 26 for 44.6%/47.6%). Plasma levels of lipoprotein (a) were increased in female patients only (27.5%). Out of keeping with previous findings in patients with chronic tinnitus, IL-6, IL-10, and TNF-α-levels were not elevated in our sample—nor did patients yield elevated rates of mean platelet volumes or neutrophil-to-lymphocyte ratios.
Considering both direct medical and indirect psychobehavioural pathways, these metabolic markers may reflect either (a) primary pathophysiological factors contributing to chronic tinnitus (i.e., through vascular or inflammatory processes that may affect otological processes [58,59,60,61]) or (b) secondary factors that might be associated with patients’ attempts to regulate distressing psychological states, such as ‘unhealthy’ dietary intake or sedentary behaviors [62,63,64,65,66].
Overall, our findings point to an unfavorable vascular-metabolic situation in these chronic tinnitus patients that may require special monitoring. Future studies will have to investigate whether pharmacological treatment of vascular risk, inflammation [67] or enzymatic dysregulation [68,69] might be helpful for some patients. Any such approaches, however, ought to consider wider emotional and psychobehavioural influences [70].

4.2. Oxidative Stress

Previous studies suggested possible interrelationships of oxidative stress- and mental-health-related processes, as well as a higher degree of oxidative stress and reduced antioxidative capacity in patients with chronic tinnitus [21]. Moreover, in patients with chronic tinnitus and hearing loss, oxidative stress has been suggested to potentially contribute to tinnitus onset through facilitating hair cell death or cochlear damage [71,72].
In the present study, redox parameters were frequently depleted in both female and male tinnitus patients (superoxide–dismutase 1 [98.0/91.8%] and ubiquinone [Q10] [43.1/33.7%]). Plasma levels of zinc were decreased in men only (30.6%). As a possible explanation for a loss of antioxidant factors, patients’ kidney function (GFR) was decreased in 50% of women and 51% of men. A higher relative proportion of monocytes was observed in 24.5% of men and in 10.8% of the women.
Superoxide-dismutase is a key antioxidant enzyme, which defends cells against oxidative stress. Ubiquinone (Q10) functions as an antioxidant co-enzyme by preventing lipid peroxidation in mitochondria or cell membranes [73,74]. Heightened oxidative stress and impaired defense processes might facilitate inflammatory responses or mitochondrial dysfunction, ultimately influencing neurotransmission and clinical symptom presentations [75,76].
Oxidative stress has been associated with a variety of psychological conditions, including depression [77,78,79], post-traumatic difficulties [80], and psychosis-spectrum conditions [81]. For the latter, the ‘oxidative stress hypothesis’ suggests that oxidative damage to lipids, proteins, or DNA might be associated with self-perpetuating changes in enzymatic and non-enzymatic antioxidant systems that may mediate “behavioral and molecular anomalies … associated with schizophrenia” [82]. Some evidence further suggests that superoxide–dismutase may be a trait- rather than a state marker for psychotic-spectrum experiences, as both acutely relapsed and stable outpatients were found to yield decreased levels of this enzyme [75]. Lower iron levels (e.g., zinc)—as found in men in the present sample—have further been shown to facilitate oxidative stress and potentially associated inflammatory processes [83], mood-related difficulties [84], or noise-triggered stress responses [85]. Seen from this perspective, previous studies that reported an association between zinc and tinnitus severity [21,22] may be interpreted within an oxidative stress framework that associates oxidative stress markers with audiologically triggered or general psychological distress in the experiences of chronic tinnitus or psychosis-spectrum conditions. Whilst previous research has attempted to delineate tinnitus from acoustic hallucinations by contrasting the former as ‘unorganized’ [86,87,88], other researchers have argued for a joint conceptualization [89].
Overall, the observed reductions in oxidative stress markers may reflect psychobiological states that underlie transdiagnostically relevant subjective experiences of perceived stress. Speculatively, oxidative stress might confer a trait vulnerability to psychoaudiological misperception or mood-mediated inflammatory epiphenomena of psychological distress in patients with chronic tinnitus or other psychological conditions.

4.3. Perceived Stress

Age- and tinnitus-related distress-adjusted linear regression analyses investigated the extent to which perceived stress influenced blood parameters in female and male patients with chronic tinnitus. As shown in Table 6 (Figure S1), we observed only marginal associations with the blood parameter conspicuities described before. Rather, associations with perceived stress were found for blood parameters within their respective reference ranges. Whilst somewhat unexpected, this finding is to be interpreted in the context of only mild overall elevation of perceived stress in the current sample.
Overall, women but not men showed positive associations between perceived stress and oxidative stress and anemic markers, whilst men but not women showed signs of worry-associated immunosuppressive processes, as reflected in inverse influences of perceived stress on fibrinogen and ferritin levels alongside positive associations with basophils [90]. Whilst preliminary, these data are in agreement with previous observations, according to which perceived stress may influence hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and red blood cell distribution width (RDW_CV; [91]) values. Moreover, these results are in keeping with previous research suggesting that chronic stress exposure might be associated with oxidative damage in women [92], particularly if associated with maladaptive lifestyle behaviors [93]. Positive associations between perceived stress and hematological markers were previously observed in healthy individuals [91,94,95], and have also been linked to acoustic trauma [96]—suggesting possibilities of direct or indirect (i.e., stress-mediated) effects of aversive noise exposure on hematological markers. Contrary to expectations, we observed negative associations between perceived stress levels and inflammatory markers in men but not women. Whilst the majority of published studies report positive associations between perceived stress and inflammatory markers [97,98], some small studies reported inverse relations in the context of heightened vascular risk [99]. The reasons for these gender differences remain mostly unknown [100], and future studies are needed to replicate and extend on these findings.
The present study has important limitations. First, the blood parameters were only measured once, and the study design disallowed for the investigation of time-related fluctuations, comparisons with healthy or non-tinnitus control groups, or effects of psychological or pharmacological interventions. Second, both the investigated index symptom (‘chronic tinnitus’) and the observed metabolic effects may be caused or confounded by a large number of unmeasured influences. Third, whilst we computed separate linear regression models, biomarkers are likely woven into complex interaction networks that need to be identified and investigated jointly in high-powered studies [101]. Therefore, the presented data must be interpreted as exploratory—offering preliminary pointers for possible candidate biomarkers of perceived stress in chronic tinnitus. Moreover, future studies ought to more carefully control sample characteristics, thereby allowing for in-detail differentiations of the effects of perceived stress on biomarkers across various levels of emotional distress and related constructs such as anxiety or depression. Notwithstanding, the current study features a relatively large clinical sample, and provides first indications of vascular–metabolic risk alongside immunosuppressive effects in patients with chronic tinnitus.

5. Conclusions

The results of the present study highlight possible interactions between vascular-metabolic risk factors and perceived stress, which may have a reinforcing role in facilitating or maintaining chronic tinnitus symptomatology. Respective pathways, however, are unclear. To prevent or treat perceived stress-related phenomena, including, but not limited to chronic tinnitus, treatment planning ought to apply a multidisciplinary view with psychological and medical professionals intervening at the intersection of vascular–metabolic risk factors, oxidative-stress-related influences, and psychological affective-behavioural (lifestyle-related) influences, whose functions need to be individually conceptualized [44,102,103,104].

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/nu14112256/s1: Figure S1: Perceived stress and blood parameters.

Author Contributions

Conceptualization, B.M., S.K., P.B.; methodology, B.B., M.W., P.B., B.M.; formal analysis, B.B., S.K., M.W.; data curation, S.K., A.N., P.B., M.W., B.B.; writing—original draft preparation, B.B.; writing—review and editing, B.B., M.W., P.B., M.R., B.M.; visualization, B.B.; supervision, B.M.; funding acquisition, B.M. All authors have read and agreed to the published version of the manuscript.

Funding

We wish to acknowledge the Heinz und Heide Duerr Foundation, whose generous support helped to conduct the present research and increase our knowledge of the field.

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki, and approved by the Ethics Committee of Charité Universitätsmedizin Berlin (No: EA1/115/15).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

As per Charité Universitaetsmedizin Berlin’s ethics committee, unfortunately we cannot make the data public without restrictions because we did not obtain patients’ consent to do so at the time. Nevertheless, interested researchers can contact the directorate of the Tinnitus Center Charité Universitaetsmedizin Berlin with data access requests ([email protected]) or the Charité’ s Open Data and Research Data Management Officer Dr. Evgeny Bobrov ([email protected]).

Acknowledgments

We extend our thanks to the HNO-Forschungslabor (ENT-Research Laboratory), Universitätsmedizin Berlin, 10117 Germany, for their reliable support and technical assistance.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Biswas, R.; Hall, D.A. Epidemiology of tinnitus. Otolaryngol. Clin. N. Am. 2020, 36, 239–248. [Google Scholar]
  2. McCormack, A.; Edmondson-Jones, M.; Somerset, S.; Hall, D. A systematic review of the reporting of tinnitus prevalence and severity. Hear. Res. 2016, 337, 70–79. [Google Scholar] [CrossRef] [PubMed]
  3. Phillips, J.S.; McFerran, D.J.; Hall, D.A.; Hoare, D.J. The natural history of subjective tinnitus in adults: A systematic review and meta-analysis of no-intervention periods in controlled trials. Laryngoscope 2018, 128, 217–227. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Wallhäusser-Franke, E.; D’Amelio, R.; Glauner, A.; Delb, W.; Servais, J.J.; Hörmann, K.; Repik, I. Transition from acute to chronic tinnitus: Predictors for the development of chronic distressing tinnitus. Front. Neurol. 2017, 8, 605. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Biehl, R.; Boecking, B.; Brueggemann, P.; Grosse, R.; Mazurek, B. Personality traits, perceived stress, and tinnitus-related distress in patients with chronic tinnitus: Support for a vulnerability-stress model. Front. Psychol. 2020, 10, 3093. [Google Scholar] [CrossRef]
  6. Hiller, W.; Goebel, G. Co-morbidity of psychological disorders in patients with complex chronic tinnitus. In Tinnitus–Psychosomatic Aspects of Complex Chronic Tinnitus; Quintessence Publishing Co Ltd.: London, UK, 1998; pp. 63–85. [Google Scholar]
  7. McKenna, L.; Handscomb, L.; Hoare, D.J.; Hall, D.A. A Scientific cognitive-behavioral model of tinnitus: Novel conceptualizations of tinnitus distress. Front. Neurol. 2014, 5, 196. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Haider, H.F.; Hoare, D.J.; Ribeiro, S.F.; Ribeiro, D.; Caria, H.; Trigueiros, N.; Borrego, L.M.; Szczepek, A.J.; Papoila, A.L.; Elarbed, A. Evidence for biological markers of tinnitus: A systematic review. Prog. Brain Res. 2021, 262, 345–398. [Google Scholar]
  9. Haider, H.F. Tinnitus, Biomarkers and Quality of Life in an Older Population; RUN: Lisboa, Portugal, 2019. [Google Scholar]
  10. Jackson, R.; Vijendren, A.; Phillips, J. Objective Measures of Tinnitus: A Systematic Review. Otol. Neurotol. 2019, 40, 154–163. [Google Scholar] [CrossRef]
  11. McFerran, D.J.; Stockdale, D.; Holme, R.; Large, C.H.; Baguley, D.M. Why is there no cure for tinnitus? Front. Neurosci. 2019, 13, 802. [Google Scholar] [CrossRef] [Green Version]
  12. Kang, D.-W.; Kim, S.-S.; Park, D.-C.; Kim, S.-H.; Yeo, S.-G. Objective and measurable biomarkers in chronic subjective tinnitus. Int. J. Mol. Sci. 2021, 22, 6619. [Google Scholar] [CrossRef] [PubMed]
  13. Kim, H.-J.; Lee, H.-J.; An, S.-Y.; Sim, S.; Park, B.; Kim, S.W.; Lee, J.S.; Hong, S.K.; Choi, H.G. Analysis of the prevalence and associated risk factors of tinnitus in adults. PLoS ONE 2015, 10, e0127578. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Pulec, J.L.; Pulec, M.B.; Ignacio, M.H. Progressive sensorineural hearing loss, subjective tinnitus and vertigo caused by elevated blood lipids. Ear. Nose. Throat, J. 1997, 76, 716–730. [Google Scholar] [CrossRef]
  15. Weber, C.; Arck, P.; Mazurek, B.; Klapp, B.F. Impact of a relaxation training on psychometric and immunologic parameters in tinnitus sufferers. J. Psychosom. Res. 2002, 52, 29–33. [Google Scholar] [CrossRef]
  16. Ulusoy, B.; Bozdemir, K.; Akyol, M.; Mise, H.I.; Kutluhan, A.; Korkmaz, M.H. Investigation of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and mean platelet volume in patients with tinnitus. J. Laryngol. Otol. 2018, 132, 129. [Google Scholar] [CrossRef]
  17. Yildiz, S.; Karaca, H.; Toros, S.Z. Mean platelet volume and neutrophil to lymphocyte ratio in patients with tinnitus: A case-control study. Braz. J. Otorhinolaryngol. 2020, 88, 155–160. [Google Scholar] [CrossRef]
  18. Ozbay, I.; Kahraman, C.; Balikci, H.H.; Kucur, C.; Kahraman, N.K.; Ozkaya, D.P.; Oghan, F. Neutrophil-to-lymphocyte ratio in patients with severe tinnitus: Prospective, controlled clinical study. J. Laryngol. Otol. 2015, 129, 544–547. [Google Scholar] [CrossRef]
  19. Bayram, A.; Yaşar, M.; Doğan, M.; Güneri, E.; Özcan, İ. Assessment of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and mean platelet volume in patients with tinnitus. ENT Updates 2015, 5, 129–132. [Google Scholar] [CrossRef]
  20. Yüksel, F.; Karatas, D. Can platelet indices be new biomarkers for subjective tinnitus? J. Craniofac. Surg. 2016, 27, e420–e424. [Google Scholar] [CrossRef]
  21. Coelho, C.B.; Tyler, R.; Hansen, M. Zinc as a possible treatment for tinnitus. Prog. Brain Res. 2007, 166, 279–285. [Google Scholar]
  22. Person, O.C.; Puga, M.E.; da Silva, E.M.; Torloni, M.R. Zinc supplementation for tinnitus. Cochrane Database Syst. Rev. 2016, 11, 1465–1858. [Google Scholar] [CrossRef]
  23. Aldwin, C.M. Stress, Coping, and Development: An Integrative Perspective; Guilford Press: New York, NY, USA, 2007. [Google Scholar]
  24. Newman, C.W.; Jacobson, G.P.; Spitzer, J.B. Development of the tinnitus handicap inventory. Arch. Otolaryngol. Neck Surg. 1996, 122, 143–148. [Google Scholar] [CrossRef] [PubMed]
  25. McCombe, A.; Baguley, D.; Coles, R.; McKenna, L.; McKinney, C.; Windle-Taylor, P. Guidelines for the Grading of Tinnitus Severity: The Results of a Working Group Commissioned by the British Association of Otolaryngologists, Head and Neck Surgeons, 1999. Clin. Otolaryngol. Allied Sci. 2001, 26, 388–393. [Google Scholar] [CrossRef] [PubMed]
  26. Malouff, J.M.; Schutte, N.S.; Zucker, L.A. Tinnitus-related distress: A review of recent findings. Curr. Psychiatry Rep. 2011, 13, 31–36. [Google Scholar] [CrossRef] [PubMed]
  27. Greimel, K.V.; Leibetseder, M.; Unterrainer, J.; Albegger, K. Can tinnitus be measured? Methods for assessment of tinnitus-specific disability and presentation of the tinnitus disability questionnaire. HNO 1999, 47, 196. [Google Scholar] [CrossRef] [PubMed]
  28. Hallam, R.S.; Jakes, S.C.; Hinchcliffe, R. Cognitive Variables in Tinnitus Annoyance. Br. J. Clin. Psychol. 1988, 27, 213–222. [Google Scholar] [CrossRef]
  29. Halford, J.B.; Anderson, S.D. Anxiety and Depression in Tinnitus Sufferers. J. Psychosom. Res. 1991, 35, 383–390. [Google Scholar] [CrossRef]
  30. Andersson, G.; Lyttkens, L.; Larsen, H.C. Distinguishing Levels of Tinnitus Distress. Clin. Otolaryngol. Allied Sci. 1999, 24, 404–410. [Google Scholar] [CrossRef]
  31. Cohen, S.; Kamarck, T.; Mermelstein, R. A Global Measure of Perceived Stress. J. Health Soc. Behav. 1983, 24, 385–396. [Google Scholar] [CrossRef]
  32. Boecking, B.; von Sass, J.; Sieveking, A.; Schaefer, C.; Brueggemann, P.; Rose, M.; Mazurek, B. Tinnitus-Related Distress and Pain Perceptions in Patients with Chronic Tinnitus–Do Psychological Factors Constitute a Link? PLoS ONE 2020, 15, e0234807. [Google Scholar] [CrossRef]
  33. Boecking, B.; Rose, M.; Brueggemann, P.; Mazurek, B. Two Birds with One Stone.–Addressing Depressive Symptoms, Emotional Tension and Worry Improves Tinnitus-Related Distress and Affective Pain Perceptions in Patients with Chronic Tinnitus. PLoS ONE 2021, 16, e0246747. [Google Scholar] [CrossRef]
  34. Schmitt, C.; Patak, M.; Kröner-Herwig, B. Stress and the Onset of Sudden Hearing Loss. Int. Tinnitus J. 2000, 6, 41–49. [Google Scholar] [PubMed]
  35. Heinecke, K.; Weise, C.; Schwarz, K.; Rief, W. Physiological and Psychological Stress Reactivity in Chronic Tinnitus. J. Behav. Med. 2008, 31, 179–188. [Google Scholar] [CrossRef] [PubMed]
  36. Budd, R.J.; Pugh, R. The relationship between locus of control, tinnitus severity, and emotional distress in a group of tinnitus sufferers. J. Psychosom. Res. 1995, 39, 1015–1018. [Google Scholar] [CrossRef]
  37. Van Munster, J.J.; van der Valk, W.H.; Stegeman, I.; Lieftink, A.F.; Smit, A.L. The relationship of tinnitus distress with personality traits: A systematic review. Front. Neurol. 2020, 11, 225. [Google Scholar] [CrossRef]
  38. Buscemi, V.; Chang, W.-J.; Liston, M.B.; McAuley, J.H.; Schabrun, S.M. The Role of Perceived Stress and Life Stressors in the Development of Chronic Musculoskeletal Pain Disorders: A Systematic Review. J. Pain 2019, 20, 1127–1139. [Google Scholar] [CrossRef]
  39. Møller, A.R. Similarities between Tinnitus and Pain. In Textbook of Tinnitus; Springer: New York, NY, USA, 2011; pp. 113–120. [Google Scholar]
  40. Tonndorf, J. The Analogy between Tinnitus and Pain: A Suggestion for a Physiological Basis of Chronic Tinnitus. Hear. Res. 1987, 28, 271–275. [Google Scholar] [CrossRef]
  41. Jastreboff, P.J.; Gray, W.C.; Gold, S.L. Neurophysiological Approach to Tinnitus Patients. Am. J. Otol. 1996, 17, 236–240. [Google Scholar]
  42. Scott, B.; Lindberg, P.; Melin, L.; Lyttkens, L. Predictors of Tinnitus Discomfort, Adaptation and Subjective Loudness. Br. J. Audiol. 1990, 24, 51–62. [Google Scholar] [CrossRef]
  43. Juster, R.-P.; McEwen, B.S.; Lupien, S.J. Allostatic load biomarkers of chronic stress and impact on health and cognition. Neurosci. Biobehav. Rev. 2010, 35, 2–16. [Google Scholar] [CrossRef]
  44. McEwen, B.S. Mood disorders and allostatic load. Biol. Psychiatry 2003, 54, 200–207. [Google Scholar] [CrossRef]
  45. Ng, F.; Berk, M.; Dean, O.; Bush, A.I. Oxidative stress in psychiatric disorders: Evidence base and therapeutic implications. Int. J. Neuropsychopharmacol. 2008, 11, 851–876. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Bartz, D.; Chitnis, T.; Kaiser, U.B.; Rich-Edwards, J.W.; Rexrode, K.M.; Pennell, P.B.; Goldstein, J.M.; O’Neal, M.A.; LeBoff, M.; Behn, M. Clinical advances in sex-and gender-informed medicine to improve the health of all: A review. JAMA Intern. Med. 2020, 180, 574–583. [Google Scholar] [CrossRef] [PubMed]
  47. Mauvais-Jarvis, F.; Merz, N.B.; Barnes, P.J.; Brinton, R.D.; Carrero, J.-J.; DeMeo, D.L.; De Vries, G.J.; Epperson, C.N.; Govindan, R.; Klein, S.L. Sex and Gender: Modifiers of Health, Disease, and Medicine. Lancet 2020, 396, 565–582. [Google Scholar] [CrossRef]
  48. Fliege, H.; Rose, M.; Arck, P.; Levenstein, S.; Klapp, B.F. Validierung Des “Perceived Stress Questionnaire” (PSQ) an Einer Deutschen Stichprobe. [Validation of the “Perceived Stress Questionnaire” (PSQ) in a German Sample]. Diagnostica 2001, 47, 142–152. [Google Scholar] [CrossRef]
  49. Fliege, H.; Rose, M.; Arck, P.; Walter, O.B.; Kocalevent, R.-D.; Weber, C.; Klapp, B.F. The Perceived Stress Questionnaire (PSQ) reconsidered: Validation and reference values from different clinical and healthy adult samples. Psychosom. Med. 2005, 67, 78–88. [Google Scholar] [CrossRef] [PubMed]
  50. Goebel, G.; Hiller, W. Tinnitus-Fragebogen: (TF); Ein Instrument Zur Erfassung von Belastung Und Schweregrad Bei Tinnitus; Handanweisung; Hogrefe, Verlag für Psychologie: Göttingen, Germany, 1998. [Google Scholar]
  51. Hallam, R.S. Manual of the Tinnitus Questionnaire (TQ); Psychological Corporation: London, UK, 1996. [Google Scholar]
  52. Gerhards, F.; Brehmer, D.; Etzkorn, M. Dimensionalität Des Tinnitus-Fragebogens. Verhaltenstherapie 2004, 14, 265–271. [Google Scholar] [CrossRef]
  53. Jacquemin, L.; Mertens, G.; Van de Heyning, P.; Vanderveken, O.M.; Topsakal, V.; De Hertogh, W.; Michiels, S.; Van Rompaey, V.; Gilles, A. Sensitivity to Change and Convergent Validity of the Tinnitus Functional Index (TFI) and the Tinnitus Questionnaire (TQ): Clinical and Research Perspectives. Hear. Res. 2019, 382, 107796. [Google Scholar] [CrossRef]
  54. Biesinger, E.; Heiden, C.; Greimel, V.; Lendle, T.; Höing, R.; Albegger, K. Strategien in Der Ambulanten Behandlung Des Tinnitus. HNO 1998, 46, 157–169. [Google Scholar] [CrossRef]
  55. Hiller, W.; Goebel, G.; Rief, W. Reliability of self-rated tinnitus distress and association with psychological symptom patterns. Br. J. Clin. Psychol. 1994, 33, 231–239. [Google Scholar] [CrossRef]
  56. Armstrong, R.A. When to use the bonferroni correction. Ophthalmic Physiol. Opt. 2014, 34, 502–508. [Google Scholar] [CrossRef]
  57. Drezner, Z.; Drezner, T.D. A Remedy for the overzealous bonferroni technique for multiple statistical tests. Bull. Ecol. Soc. Am. 2016, 97, 91–98. [Google Scholar] [CrossRef]
  58. Jalali, M.M.; Azgomi, M.N. Metabolic Syndrome Components and Sudden Sensorineural Hearing Loss: A Case–Control Study. Eur. Arch. Otorhinolaryngol. 2020, 277, 1023–1029. [Google Scholar] [CrossRef] [PubMed]
  59. Wang, S.; Ye, Q.; Pan, Y. Serum non-high-density lipoprotein cholesterol is associated with the risk of sudden sensorineural hearing loss. Medicine 2020, 99, e19175. [Google Scholar] [CrossRef] [PubMed]
  60. Doo, J.G.; Kim, D.; Kim, Y.; Yoo, M.C.; Kim, S.S.; Ryu, J.; Yeo, S.G. Biomarkers suggesting favorable prognostic outcomes in sudden sensorineural hearing loss. Int. J. Mol. Sci. 2020, 21, 7248. [Google Scholar] [CrossRef] [PubMed]
  61. Rudack, C.; Langer, C.; Stoll, W.; Rust, S.; Walter, M. Vascular risk factors in sudden hearing loss. Thromb. Haemost. 2006, 95, 454–461. [Google Scholar] [CrossRef]
  62. Forsyth, A.; Deane, F.P.; Williams, P. A Lifestyle intervention for primary care patients with depression and anxiety: A randomised controlled trial. Psychiatry Res. 2015, 230, 537–544. [Google Scholar] [CrossRef]
  63. Klatzkin, R.R.; Baldassaro, A.; Rashid, S. Physiological responses to acute stress and the drive to eat: The impact of perceived life stress. Appetite 2019, 133, 393–399. [Google Scholar] [CrossRef]
  64. Stubbs, B.; Vancampfort, D.; Firth, J.; Schuch, F.B.; Hallgren, M.; Smith, L.; Gardner, B.; Kahl, K.G.; Veronese, N.; Solmi, M. Relationship between sedentary behavior and depression: A mediation analysis of influential factors across the lifespan among 42,469 people in low-and middle-income countries. J. Affect. Disord. 2018, 229, 231–238. [Google Scholar] [CrossRef] [Green Version]
  65. Teasdale, S.B.; Ward, P.B.; Rosenbaum, S.; Samaras, K.; Stubbs, B. Solving a Weighty Problem: Systematic review and meta-analysis of nutrition interventions in severe mental illness. Br. J. Psychiatry 2017, 210, 110–118. [Google Scholar] [CrossRef] [Green Version]
  66. Epstein, L.H.; Leddy, J.J.; Temple, J.L.; Faith, M.S. Food Reinforcement and Eating: A Multilevel Analysis. Psychol. Bull. 2007, 133, 884–906. [Google Scholar] [CrossRef]
  67. Ridker, P.M.; Everett, B.M.; Thuren, T.; MacFadyen, J.G.; Chang, W.H.; Ballantyne, C.; Fonseca, F.; Nicolau, J.; Koenig, W.; Anker, S.D. Antiinflammatory therapy with canakinumab for atherosclerotic disease. N. Engl. J. Med. 2017, 377, 1119–1131. [Google Scholar] [CrossRef] [PubMed]
  68. Akbari, A.; Mobini, G.R.; Agah, S.; Morvaridzadeh, M.; Omidi, A.; Potter, E.; Fazelian, S.; Ardehali, S.H.; Daneshzad, E.; Dehghani, S. Coenzyme Q10 supplementation and oxidative stress parameters: A systematic review and meta-analysis of clinical trials. Eur. J. Clin. Pharmacol. 2020, 76, 1483–1499. [Google Scholar] [CrossRef] [PubMed]
  69. Ciorba, A.; Iannini, V.; Soliani, M.; Tosin, E.; Aimoni, C.; Mazzoli, M.; Pastore, A. Alfa-Lipoic Acid and Superoxide Dismutase for the Treatment of Subjective Chronic Idiopathic Tinnitus: A Pilot Study. Otorinolaringologia 2015, 65, 71–76. [Google Scholar]
  70. Gold, S.M.; Köhler-Forsberg, O.; Moss-Morris, R.; Mehnert, A.; Miranda, J.J.; Bullinger, M.; Steptoe, A.; Whooley, M.A.; Otte, C. Comorbid Depression in Medical Diseases. Nat. Rev. Dis. Primer 2020, 6, 69. [Google Scholar] [CrossRef]
  71. Baker, K.; Staecker, H. Low dose oxidative stress induces mitochondrial damage in hair cells. Anat. Rec. Adv. Integr. Anat. Evol. Biol. 2012, 295, 1868–1876. [Google Scholar] [CrossRef]
  72. Ciorba, A.; Bianchini, C.; Pastore, A.; Mazzoli, M. Pathogenesis of tinnitus: Any role for oxidative stress? J. Int. Adv. Otol. 2013, 9, 249. [Google Scholar]
  73. Ernster, L.; Dallner, G. Biochemical, physiological and medical aspects of ubiquinone function. Biochim. Biophys. Acta BBA Mol. Basis Dis. 1995, 1271, 195–204. [Google Scholar] [CrossRef] [Green Version]
  74. Nordberg, J.; Arnér, E.S. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic. Biol. Med. 2001, 31, 1287–1312. [Google Scholar] [CrossRef]
  75. Flatow, J.; Buckley, P.; Miller, B.J. Meta-analysis of oxidative stress in schizophrenia. Biol. Psychiatry 2013, 74, 400–409. [Google Scholar] [CrossRef] [Green Version]
  76. Yao, J.K.; Keshavan, M.S. Antioxidants, redox signaling, and pathophysiology in schizophrenia: An integrative view. Antioxid. Redox Signal. 2011, 15, 2011–2035. [Google Scholar] [CrossRef]
  77. Chung, C.P.; Schmidt, D.; Stein, C.M.; Morrow, J.D.; Salomon, R.M. Increased oxidative stress in patients with depression and its relationship to treatment. Psychiatry Res. 2013, 206, 213–216. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  78. Herken, H.; Gurel, A.; Selek, S.; Armutcu, F.; Ozen, M.E.; Bulut, M.; Kap, O.; Yumru, M.; Savas, H.A.; Akyol, O. Adenosine deaminase, nitric oxide, superoxide-dismutase, and xanthine oxidase in patients with major depression: Impact of antidepressant treatment. Arch. Med. Res. 2007, 38, 247–252. [Google Scholar] [CrossRef] [PubMed]
  79. Maes, M.; Galecki, P.; Chang, Y.S.; Berk, M. A Review on the Oxidative and Nitrosative Stress (O&NS) pathways in major depression and their possible contribution to the (neuro) degenerative processes in that illness. Prog. Neuropsychopharmacol. Biol. Psychiatry 2011, 35, 676–692. [Google Scholar] [PubMed]
  80. Borovac Štefanović, L.; Kalinić, D.; Mimica, N.; Beer Ljubić, B.; Aladrović, J.; Mandelsamen Perica, M.; Ćurić, M.; Grošić, P.F.; Delaš, I. Oxidative status and the severity of clinical symptoms in patients with post-traumatic stress disorder. Ann. Clin. Biochem. 2015, 52, 95–104. [Google Scholar] [CrossRef] [Green Version]
  81. Coughlin, J.M.; Ishizuka, K.; Kano, S.I.; Edwards, J.A.; Seifuddin, F.T.; Shimano, M.A.; Daley, E.L.; Zandi, P.P.; Leweke, F.M.; Cascella, N.G. Marked Reduction of Soluble Superoxide Dismutase-1 (SOD1) in cerebrospinal fluid of patients with recent-onset schizophrenia. Mol. Psychiatry 2013, 18, 10–11. [Google Scholar] [CrossRef] [Green Version]
  82. Bitanihirwe, B.K.; Woo, T.-U.W. Oxidative stress in schizophrenia: An integrated approach. Neurosci. Biobehav. Rev. 2011, 35, 878–893. [Google Scholar] [CrossRef] [Green Version]
  83. Hosseini, R.; Ferns, G.A.; Sahebkar, A.; Mirshekar, M.A.; Jalali, M. Zinc supplementation is associated with a reduction in serum markers of inflammation and oxidative stress in adults: A systematic review and meta-analysis of randomized controlled trials. Cytokine 2021, 138, 155396. [Google Scholar] [CrossRef]
  84. Yosaee, S.; Clark, C.C.; Keshtkaran, Z.; Ashourpour, M.; Keshani, P.; Soltani, S. Zinc in depression: From development to treatment: A comparative/dose response meta-analysis of observational studies and randomized controlled trials. Gen. Hosp. Psychiatry 2020, 74, 110–117. [Google Scholar] [CrossRef]
  85. Daiber, A.; Kröller-Schön, S.; Oelze, M.; Hahad, O.; Li, H.; Schulz, R.; Steven, S.; Münzel, T. Oxidative stress and inflammation contribute to traffic noise-induced vascular and cerebral dysfunction via uncoupling of nitric oxide synthases. Redox Biol. 2020, 34, 101506. [Google Scholar] [CrossRef]
  86. Kreuzer, P.M.; Vielsmeier, V.; Langguth, B. Chronic tinnitus: An interdisciplinary challenge. Dtsch. Ärztebl. Int. 2013, 110, 278. [Google Scholar]
  87. Langguth, B.; Kreuzer, P.M.; Kleinjung, T.; De Ridder, D. Tinnitus: Causes and clinical management. Lancet Neurol. 2013, 12, 920–930. [Google Scholar] [CrossRef]
  88. Nam, E.-C. Is it necessary to differentiate tinnitus from auditory hallucination in schizophrenic patients? J. Laryngol. Otol. 2005, 119, 352–355. [Google Scholar] [CrossRef] [PubMed]
  89. Sperling, W.; Mueller, H.; Kornhuber, J.; Biermann, T. Is tinnitus an acoasm? Med. Hypotheses 2011, 77, 216–219. [Google Scholar] [CrossRef] [PubMed]
  90. Klein, S.L.; Flanagan, K.L. Sex differences in immune responses. Nat. Rev. Immunol. 2016, 16, 626. [Google Scholar] [CrossRef]
  91. Maes, M.; Van Der Planken, M.; Van Gastel, A.; Bruyland, K.; Van Hunsel, F.; Neels, H.; Hendriks, D.; Wauters, A.; Demedts, P.; Janca, A. Influence of academic examination stress on hematological measurements in subjectively healthy volunteers. Psychiatry Res. 1998, 80, 201–212. [Google Scholar] [CrossRef]
  92. Aschbacher, K.; O’Donovan, A.; Wolkowitz, O.M.; Dhabhar, F.S.; Su, Y.; Epel, E. Good stress, bad stress and oxidative stress: Insights from anticipatory cortisol reactivity. Psychoneuroendocrinology 2013, 38, 1698–1708. [Google Scholar] [CrossRef] [Green Version]
  93. Gonçalves Mota, M.P.; Santos, Z.; Soares, J.; Pereira, A.; Fonseca, S.; Peixoto, F.; Gaivão, I.; Oliveira, M. Oxidative stress function in women over 40 years of age, considering their lifestyle. Front. Endocrinol. 2017, 8, 48. [Google Scholar] [CrossRef] [Green Version]
  94. Jern, C.; Wadenvik, H.; Mark, H.; Hallgren, J.; Jern, S. Haematological changes during acute mental stress. Br. J. Haematol. 1989, 71, 153–156. [Google Scholar] [CrossRef]
  95. Segerstrom, S.C.; Miller, G.E. Psychological stress and the human immune system: A meta-analytic study of 30 years of inquiry. Psychol. Bull. 2004, 130, 601. [Google Scholar] [CrossRef] [Green Version]
  96. Salihoğlu, M.; Kurt, O.; Altundağ, A.; Çayönü, M. The Biochemical and Hematological Parameters of Patients with Acoustic Trauma Caused by Gunshot Noise. Praxis of ORL. 2014, 2, 18–22. [Google Scholar] [CrossRef] [Green Version]
  97. Gouin, J.-P.; Glaser, R.; Malarkey, W.B.; Beversdorf, D.; Kiecolt-Glaser, J. Chronic stress, daily stressors, and circulating inflammatory markers. Health Psychol. 2012, 31, 264. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  98. Marsland, A.L.; Walsh, C.; Lockwood, K.; John-Henderson, N.A. The effects of acute psychological stress on circulating and stimulated inflammatory markers: A systematic review and meta-analysis. Brain. Behav. Immun. 2017, 64, 208–219. [Google Scholar] [CrossRef] [PubMed]
  99. Mattiasson, I.; Lindgärde, F. The effect of psychosocial stress and risk factors for ischaemic heart disease on the plasma fibrinogen concentration. J. Intern. Med. 1993, 234, 45–51. [Google Scholar] [CrossRef] [PubMed]
  100. Bekhbat, M.; Neigh, G.N. Sex differences in the neuro-immune consequences of stress: Focus on depression and anxiety. Brain. Behav. Immun. 2018, 67, 1–12. [Google Scholar] [CrossRef]
  101. Tai, A.M.; Albuquerque, A.; Carmona, N.E.; Subramanieapillai, M.; Cha, D.S.; Sheko, M.; Lee, Y.; Mansur, R.; McIntyre, R.S. Machine learning and big data: Implications for disease modeling and therapeutic discovery in psychiatry. Artif. Intell. Med. 2019, 99, 101704. [Google Scholar] [CrossRef]
  102. Fava, G.A.; McEwen, B.S.; Guidi, J.; Gostoli, S.; Offidani, E.; Sonino, N. Clinical characterization of allostatic overload. Psychoneuroendocrinology 2019, 108, 94–101. [Google Scholar] [CrossRef]
  103. Guidi, J.; Lucente, M.; Sonino, N.; Fava, G.A. Allostatic load and its impact on health: A systematic review. Psychother. Psychosom. 2020, 90, 11–27. [Google Scholar] [CrossRef]
  104. McEwen, B.S. Allostasis and allostatic load: Implications for neuropsychopharmacology. Neuropsychopharmacology 2000, 22, 108–124. [Google Scholar] [CrossRef]
Table
Table 1. Sociodemographic data and patient characteristics (n = 169).
Table 1. Sociodemographic data and patient characteristics (n = 169).
n%
Nationality
German15879.0
Other115.5
Education
Completed junior apprenticeship4422.0
Completed senior apprenticeship2814.0
University degree9045.0
Employment ‘yes’11959.5
Relationship status
Single3115.5
Married11457.0
Divorced168.0
Widowed73.5
Duration of tinnitus
<0.5 year2110.5
0.5–1 year2512.5
1–2 years126.0
2–5 years3115.5
>5 years6633.0
Past psychotherapy ‘yes’8643.0
Table
Table 2. Means and standard deviations for the psychological indices.
Table 2. Means and standard deviations for the psychological indices.
Total
(n = 200)		 Women
(n = 102)		 Men
(n = 98)
MeanSDMeanSDMeanSD
PSQ total50.2713.1951.4412.1649.0714.16
Worries13.629.3213.7910.0413.448.56
Tension19.1416.5019.9918.0318.2614.79
Joy *16.8015.6715.8913.3117.7417.83
Demands17.0814.5517.4615.3316.7013.76
TQ total43.8019.0145.2918.2742.2619.72
* Reversely coded (i.e., the higher the better). PSQ = Perceived Stress Questionnaire; TQ = Tinnitus Questionnaire (German version).
Table
Table 3. Means and standard deviations of vascular–metabolic risk factors and frequency rates of blood parameters with increased or decreased incidence rates of ≥25%. Relevant indices are highlighted for emphasis.
Table 3. Means and standard deviations of vascular–metabolic risk factors and frequency rates of blood parameters with increased or decreased incidence rates of ≥25%. Relevant indices are highlighted for emphasis.
Total Women Men
UnitMeanSDMeanSDMeanSD
§ Weightkg78.8615.7178.5916.0479.1215.50
§ BMIkg/m226.324.4425.944.7026.704.17
Frequency% (total)Frequency% (women)Frequency% (men)
† Current smoking ‘yes‘5945.43350.82640.0
†§ Regular drinking ‘yes‘2922.31421.51523.1
§ These risk factors were only available for a subset of n = 128 patients (65% of total sample; n = 65 women; n = 63 men).† Operationalized as drinking regularly “at least weekly”. BMI = Body Mass Index (underweight: <18.5; normal: 18.5–25; overweight: 25–30; obese: >30).
Table
Table 4. Frequency rates of participants with increased or decreased vascular-metabolic blood parameters. Indices with ‘outstanding’ rates of ≥ 25% are highlighted for emphasis.
Table 4. Frequency rates of participants with increased or decreased vascular-metabolic blood parameters. Indices with ‘outstanding’ rates of ≥ 25% are highlighted for emphasis.
UnitMeanSDReference ValuesFrequency Decreased Frequency Increased
MenWomenTotal(% Total)Women(% Women)Men(% Men)Total(% Total)Women(% Women)Men(% Men)
Total cholesterolmg/dL212.3138.24<200<200------126(63.0)66(64.7)60(61.2)
Triglyceridesmg/dL124.2562.24≤200≤200------23(11.5)7(6.9)16(16.3)
HDL-cmg/dL63.2318.34≥35≥456(3)3(2.9)3(3.1)------
Non-HDL-cmg/dL149.3439.75<150<150------93(46.5)44(43.1)49(50.0)
LDL-cmg/dL137.1034.56<130<130------115(57.5)57(55.9)58(59.2)
Lipoprotein_anmol/L45.8365.34<72.0<72.0------42(21.0)28(27.5)14(14.3)
HDL-c = high-density lipoprotein; LDL-c = low-density lipoprotein; non-HDL-c = non-high-density lipoprotein.
Table
Table 5. Means, standard deviations, reference values, and frequency rates of participants with increased or decreased non-vascular-metabolic blood parameters. Indices with ‘outstanding’ rates of ≥25% are highlighted for emphasis.
Table 5. Means, standard deviations, reference values, and frequency rates of participants with increased or decreased non-vascular-metabolic blood parameters. Indices with ‘outstanding’ rates of ≥25% are highlighted for emphasis.
UnitMeanSDReference ValuesFrequency DecreasedFrequency Increased
MenWomenTotal% TotalWomen% WomenMen% MenTotal% TotalWomen% WomenMen% Men
LeukocytesnL6.581.583.9–10.53.9–10.56(3)3(2.9)3(3.1)2(1.0)--2(2.0)
Lymphocytesabsolute/nL1.960.561.10–4.501.10–4.508(4)5(4.9)3(3.1)2(1.0)----
Lymphocytes%30.437.7620.0–44.020.0–44.017(8.5)9(8.8)8(8.2)12(6.0)9(8.8)3(3.1)
Monocytesabsolute/nL0.520.160.10–0.900.10–0.90------6(3.0)1(1.0)5(5.1)
Monocytes%7.941.922.0–9.52.0–9.5------35(17.5)11(10.8)24(24.5)
Neutrophilsabsolute/nL3.871.201.50–7.701.50–7.701(0.5)1(1.0)--------
Neutrophils%58.358.7442.0–77.042.0–77.09(4.5)6(5.9)3(3.1)5(2.5)4(3.9)1(1.0)
NLRcells/μL2.100.861–3 *1–3 *------------
Immature_granulocytesabsolute/nL0.020.02<0.050<0.050------11(5.5)1(1.0)10(10.2)
Immature_granulocytes%0.360.200.0–1.00.0–1.0------4(2.0)--4(4.1)
Eosinophilsabsolute/nL0.140.100.02–0.500.02–0.503(1.5)1(1.0)2(2.0)1(0.5)--1(1.0)
Eosinophils%2.071.320.5–5.50.5–5.511(5.5)8(7.8)3(3.1)6(3.0)3(2.9)3(3.1)
Basophilsabsolute/nL0.050.020.00–0.200.00–0.20------------
Basophils%0.740.320.0–1.80.0–1.8------------
TNF-αpg/mL0.320.05<8.1<8.1------------
IL6ng/L1.831.12≤7.0≤7.0------3(1.5)1(1.0)2(2.0)
CRPmg/L1.611.72<5.0<5.0------10(5.0)7(6.9)3(3.1)
Fibrinogeng/L2.720.571.60–4.001.60–4.004(2)4(3.9)--3(1.5)2(2.0)1(1.0)
Ferritinqg/L128.2993.0930.0–400.013.0–150.04(2.0)2(2.0)2(2.0)19(9.5)14(13.7)5(5.1)
ThrombocytesnL244.3354.29150–370150–3701(0.5)--1(1.0)5(2.5)4(3.9)1(1.0)
MPVfl10.681.007.0–12.07.0–12.0------19(9.5)7(6.9)12(12.2)
Hemoglobing/dL14.401.2413.5–17.012.0–15.65(2.5)2(2.0)3(3.1)5(2.5)--5(5.1)
Hematocritl/L0.430.040.395–0.5050.355–0.4556(3.0)3(2.9)3(3.1)6(3.0)3(2.9)3(3.1)
Erythrocytespl4.820.434.3–5.83.9–5.21(0.5)--1(1.0)5(2.5)--5(5.1)
MCVfl88.333.6080.0–99.080.0–99.02(1.0)1(1.0)1(1.0)------
RDW_CV%12.790.5811.5–15.011.5–15.0------------
MCHpg29.891.2727.0–33.527.0–33.53(1.5)2(2.0)1(1.0)------
MCHCg/dL33.830.9431.5–36.031.5–36.05(2.5)4(3.9)1(1.0)3(1.5)1(1.0)2(2.0)
Superoxide–Dismutase 1ng/mL63.894.5377–53177–531190(95.0)100(98.0)90(91.8)------
Superoxide–Dismutase 2ng/mL58.9315.52>40>4016(8.0)16(15.7)--------
Lipid_Peroxidaseμmol/L64.1079.66<200<200------11(5.5)10(9.8)1(1.0)
Q10 (lipid-corrected)μmol/mmol0.230.07>0.2>0.277(38.5)44(43.1)33(33.7)------
Albuming/L46.362.5035.0–52.035.0–52.0------3(1.5)--3(3.1)
GOTU/L24.846.46<50<35------4(2.0)2(2.0)2(2.0)
GPTU/L27.9212.22<41<31------38(19.0)15(14.7)23(23.5)
Gamma_GTU/L24.6413.298–615–36------12(6.0)9(8.8)3(3.1)
GFRmL/min84.438.46>90>90101(50.5)51(50.0)50(51.0)------
Uric acidmg/dL4.931.233.6–8.22.3–6.11(0.5)--1(1.0)5(2.5)3(2.9)2(2.0)
Creatininemg/dL0.850.150.70–1.200.50–0.901(0.5)1(1.0)--1(0.5)1(1.0)--
Calciummmol/L2.340.092.15–2.502.15–2.505(2.5)3(2.9)2(2.0)------
Magnesiummmol/L0.850.050.66–1.070.66–1.07------------
Zincqmol/L12.631.7712.0–26.09.0–22.031(15.5)1(1.0)30(30.6)------
Seleniumqmol/L1.000.210.60–1.500.60–1.504(2.0)2(2.0)2(2.0)4(2.0)3(2.9)1(1.0)
Vitamin D3nmol/L65.3021.0250.0–150.050.0–150.046(23.0)24(23.5)22(22.4)------
CRP = C-reactive_protein; GFR = glomerular filtration rate; GOT = glutamate–oxalacetate–transaminase; GPT = glutamate–pyruvate–transaminase; IL6 = interleukin-6; MCH = mean corpuscular/cellular hemoglobin; MCHC = mean corpuscular hemoglobin concentration; MCV = mean corpuscular/cell volume; MPV = mean platelet volume; NLR = neutrophil/lymphocyte ratio; RDW_CV = red blood cell distribution width; TNF-α = tumor necrosis factor alpha; * reference value obtained from www.mdcalc.com/neutrophil-lymphocyte-ratio-nlr-calculator#pearls-pitfalls (accessed on 2 April 2022).
Table
Table 6. Age- and tinnitus-related distress-adjusted univariate regression analyses with perceived stress indices being regressed on blood parameter values for the total sample, female patients, and male patients with chronic tinnitus. Only significant associations are reported (* = p < 0.05; ** = p < 0.01; *** = p < 0.001).
Table 6. Age- and tinnitus-related distress-adjusted univariate regression analyses with perceived stress indices being regressed on blood parameter values for the total sample, female patients, and male patients with chronic tinnitus. Only significant associations are reported (* = p < 0.05; ** = p < 0.01; *** = p < 0.001).
Total Sample PSQ_Total Worries Tension Joy Demands
βt (3.194)βt (3.194)βt (3.194)βt (3.194)βt (3.194)
Vascular risk markersLipoprotein_a 0.141.99 *
Inflammatory markersCRP−0.19−2.12 * −0.15−2.10 *
Fibrinogen −0.16−2.36 *
Ferritin−0.19−2.11 *
MCV 0.294.27 ***0.253.58 ***0.253.40 **0.273.96 ***
MCHC −0.23−3.27 **−0.202.84 **−0.20−2.59 *−0.21−2.96 **
Oxidative stress markersUbiquinone(Q10)_lipid-corrected −0.15−2.08 *−0.22−3.14 ** −0.22−3.09 **
Selenium 0.172.29 *
Magnesium 0.152.10 *
Zinc 0.273.78 ***0.253.52 **0.222.85 **0.243.40 **
Cellular immune reponseBasophils (abs) 0.172.35 *
Basophils (%) 0.162.25 * 0.182.52 *
Female Patients PSQ_total Worries Tension Joy Demands
βt (3.98)βt (3.98)βt (3.98)βt (3.98)βt (3.98)
Vascular risk markersLipoprotein_a 0.211.98 *
Inflammatory markersFerritin 0.252.50 *0.272.86 **0.212.18 *0.222.28 *
MCV0.262.06 *0.363.64 ***0.293.01 **0.212.05 *0.343.57 **
MCHC −0.24−2.30 * −0.21−2.12 *
Hematocrit 0.222.19 *0.282.91 **0.292.95 **0.303.20 **
Hemoglobin 0.242.42 *0.242.43 *0.242.44 *
RDW_CV −0.21−2.13 *−0.20−1.99 *
Oxidative stress markersSelenium 0.212.12 *
Magnesium 0.222.23 * 0.202.07 *
Zinc 0.343.35 **0.323.23 **0.222.17 *0.333.39 **
SOD-2 0.222.16 *0.212.19 * 0.202.07 *
Male Patients PSQ_Total Worries Tension Joy Demands
βt (3.94)βt (3.94)βt (3.94)βt (3.94)βt (3.94)
Inflammatory markersCRP−0.31−2.35 *
Fibrinogen−0.34−2.63 *−0.28−2.84 **−0.26−2.60 *
Ferritin −0.26−2.54 *
MCV 0.23−2.32 * 0.292.69 **
MCHC −0.242.34 *−0.23−2.32 *−0.24−2.16 *
IL-6 0.312.87 **
Uric acid −0.24−2.33 * −0.282.74 **
Oxidative stress markersUbiquinone(Q10)_lipid-corrected −0.24−2.43 * −0.24−2.71 **
Selenium0.302.23 *
Immunological markersBasophils (abs) 0.222.13 *0.201.98 *
Basophils (%)0.382.91 **0.353.53 **0.272.77 ** 0.272.68 **
Leukocytes −0.21−2.03 *
Neutrophils (abs) −0.24−2.32 *
Neutrophils (%) −0.22−2.17 *
Liver functionGPT−0.28−2.11 *
CRP = C-reactive protein; MCV = mean corpuscular/cell volume; MCHC = mean corpuscular hemoglobin concentration; PSQ = Perceived Stress Questionnaire; RDW_CV = red blood cell distribution width; SOD-2 = Superoxide Dismutase 2; GPT = glutamate–pyruvate–transaminase; IL-6 = interleukin-6.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Boecking, B.; Klasing, S.; Walter, M.; Brueggemann, P.; Nyamaa, A.; Rose, M.; Mazurek, B. Vascular–Metabolic Risk Factors and Psychological Stress in Patients with Chronic Tinnitus. Nutrients 2022, 14, 2256. https://doi.org/10.3390/nu14112256

AMA Style

Boecking B, Klasing S, Walter M, Brueggemann P, Nyamaa A, Rose M, Mazurek B. Vascular–Metabolic Risk Factors and Psychological Stress in Patients with Chronic Tinnitus. Nutrients. 2022; 14(11):2256. https://doi.org/10.3390/nu14112256

Chicago/Turabian Style

Boecking, Benjamin, Sven Klasing, Michael Walter, Petra Brueggemann, Amarjargal Nyamaa, Matthias Rose, and Birgit Mazurek. 2022. "Vascular–Metabolic Risk Factors and Psychological Stress in Patients with Chronic Tinnitus" Nutrients 14, no. 11: 2256. https://doi.org/10.3390/nu14112256

APA Style

Boecking, B., Klasing, S., Walter, M., Brueggemann, P., Nyamaa, A., Rose, M., & Mazurek, B. (2022). Vascular–Metabolic Risk Factors and Psychological Stress in Patients with Chronic Tinnitus. Nutrients, 14(11), 2256. https://doi.org/10.3390/nu14112256

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop