The High-Efficiency Degradation of Multiple Mycotoxins by Lac-W Laccase in the Presence of Mediators
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mycotoxins Degradation by LMSs
2.1.1. AFB1 Degradation by LMSs
2.1.2. ZEN Degradation by LMSs
2.1.3. Degradation of DON, T-2 Toxin, FB1, and OTA by LMSs
2.2. The Effects of Various Conditions on ZEN Degradation by Lac-W-AS
2.3. Cytotoxicity Assay of ZEN Degradation Products
2.4. ZEN Degradation Product by Lac-W-AS
2.5. Determination of the Redox Potential of Lac-W and Lac-W-AS
2.6. Independent and Simultaneous Degradation of Mycotoxins by Lac-W-AS-ABTS
2.7. Mycotoxin Degradation in Corn Husk by Lac-W-AS-ABTS
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Chemicals, Recombined Bacteria Strain, and Mycotoxins Standard
5.2. Mycotoxins Degradation by LMS
5.3. Mycotoxin Degradation in Corn Husk
5.4. Evaluation of Mycotoxin Degradation Rates by UHPLC-MS/MS
5.5. Luminescence Inhibition Assays
5.6. Evaluation of ZEN Degradation Product by LMS
5.7. Linear Sweep Voltammetry
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jia, M.; Yu, X.; Xu, K.; Gu, X.; Harmer, N.J.; Zhao, Y.; Xiang, Y.; Sheng, X.; Li, C.; Du, X.-D.; et al. The High-Efficiency Degradation of Multiple Mycotoxins by Lac-W Laccase in the Presence of Mediators. Toxins 2024, 16, 477. https://doi.org/10.3390/toxins16110477
Jia M, Yu X, Xu K, Gu X, Harmer NJ, Zhao Y, Xiang Y, Sheng X, Li C, Du X-D, et al. The High-Efficiency Degradation of Multiple Mycotoxins by Lac-W Laccase in the Presence of Mediators. Toxins. 2024; 16(11):477. https://doi.org/10.3390/toxins16110477
Chicago/Turabian StyleJia, Mengshuang, Xiaohu Yu, Kun Xu, Xiaodan Gu, Nicholas J. Harmer, Youbao Zhao, Yuqiang Xiang, Xia Sheng, Chenglong Li, Xiang-Dang Du, and et al. 2024. "The High-Efficiency Degradation of Multiple Mycotoxins by Lac-W Laccase in the Presence of Mediators" Toxins 16, no. 11: 477. https://doi.org/10.3390/toxins16110477
APA StyleJia, M., Yu, X., Xu, K., Gu, X., Harmer, N. J., Zhao, Y., Xiang, Y., Sheng, X., Li, C., Du, X. -D., Pan, J., & Hao, W. (2024). The High-Efficiency Degradation of Multiple Mycotoxins by Lac-W Laccase in the Presence of Mediators. Toxins, 16(11), 477. https://doi.org/10.3390/toxins16110477