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Article

Contamination Status and Health Risk Assessment of 73 Mycotoxins in Four Edible and Medicinal Plants Using an Optimized QuEChERS Pretreatment Coupled with LC-MS/MS

by
Xiaojing Huang
,
Rui Feng
,
Qing Hu
,
Xiuhong Mao
and
Heng Zhou
*
Shanghai Institute for Food and Drug Control, Shanghai 201203, China
*
Author to whom correspondence should be addressed.
Toxins 2025, 17(2), 52; https://doi.org/10.3390/toxins17020052
Submission received: 17 December 2024 / Revised: 16 January 2025 / Accepted: 20 January 2025 / Published: 23 January 2025
(This article belongs to the Special Issue Biomonitoring of Mycotoxins the Silent Invaders, Their Subtle Assault on Human and Animal Health)
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Abstract

:
The current status of multi-mycotoxin contamination in edible and medicinal plants demands urgent development of high-throughput analytical methods for mycotoxin detection. In this study, a reliable and sensitive method for the simultaneous analysis of 73 mycotoxins was established and successfully applied to detect mycotoxins in 260 samples of four dual-purpose plants (lotus seed, coix seed, licorice root, and dried tangerine peel). Sample preparation involved optimized QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction combined with liquid–liquid extraction purification, and an enhanced ion pair library was established to reduce matrix interference and improve the method’s universality. Method validation demonstrated recovery rates ranging from 61.6% to 118.6% for all compounds, with relative standard deviations (RSDs) below 15%. The limits of detection (LODs) and quantification (LOQs) ranged from 0.25–12.25 μg/kg and 0.5–25 μg/kg, respectively. Based on the contamination analysis and health risk assessment using Margin of Exposure (MOE) and Hazard Index (HI) methods, we found that multi-mycotoxin contamination is highly prevalent in edible and medicinal plants, with different components being susceptible to invasion by distinct fungal genera. Seed-type plants showed high susceptibility to Aspergillus (53.3%) and Fusarium (22.2%) contamination, with MOE values below 10,000 for aflatoxins indicating potential health risks. Physical state and good storage conditions significantly influenced contamination levels, with fragmented samples showing substantially higher mycotoxin levels. Additionally, mycotoxins with associated biosynthetic metabolic pathways were frequently detected simultaneously in highly contaminated samples. Based on these findings, we recommend implementing strict moisture control during storage, maintaining intact product form where possible, and establishing comprehensive supplier qualification systems. This study provides valuable reference for monitoring mycotoxin contamination in similar plants.
Keywords:
mycotoxins; edible and medicinal plants; QuEChERS; UHPLC-MS/MS; contamination status; health risk assessment
Key Contribution: This study developed an optimized QuEChERS method and effectively applied it to four edible and medicinal plants, encompassing a comprehensive range of monitored and regulated mycotoxins, totaling 73 different types under surveillance. Multiple mycotoxin contamination is widespread in dual-purpose plants, and mycotoxins with associated biosynthetic metabolic pathways were frequently co-detected in highly contaminated samples. Seeds demonstrated higher health risks, showing susceptibility to aflatoxins and zearalenone contamination. The exceptionally high contamination rate of fumonisins in coix seeds suggests the need for continuous monitoring.

1. Introduction

Mycotoxins, as secondary metabolites produced by fungi, readily contaminate various food matrices, including foodstuffs, oils, and traditional Chinese medicines (TCMs) [1,2,3]. To date, over 400 mycotoxins have been identified and isolated, with several, such as aflatoxins and ochratoxins, being demonstrated to possess severe toxic effects, including the induction of hepatocellular carcinoma and various diseases affecting the urinary and gastrointestinal systems [1,4,5]. Furthermore, co-contamination by multiple mycotoxins may result in synergistic toxic effects. For instance, the combined toxicity of aflatoxin B1, zearalenone, and deoxynivalenol mixture exhibits enhanced hepatotoxicity in rat hepatocytes compared to their individual effects. Furthermore, certain matrices are susceptible to contamination by both masked and emerging mycotoxins [6,7,8]. These circumstances pose significant threats to public health [9,10,11]. Consequently, numerous countries and organizations have established limits for mycotoxins. For example, the European Commission recently issued Commission Regulation (EU) No 2023/915 of 25 April 2023 on the maximum levels for certain contaminants in food, repealing Regulation (EC) No 1881/2006, which now stipulates maximum levels for sixteen mycotoxins (including three newly added ones) in foodstuffs.
Globally, over two billion people rely on TCMs for their health benefits and, with the increasing emphasis on health and wellness, the consumption of edible TCMs has risen substantially [12,13,14,15]. However, regulatory standards for mycotoxin limits in plants lag behind those for food products, primarily due to insufficient detection methods, contamination data, and related risk assessments. Current high-throughput mycotoxin detection methods predominantly focus on food matrices, typically employing a QuEChERS or “dilute and shoot” method for sample preparation. For instance, Michael Sulyok et al. developed a method combining direct extraction with LC-MS/MS to determine 39 mycotoxins in wheat and corn [16,17]. Similarly, Ádám Tölgyesi et al. developed a novel LC-MS/MS multi-method for the simultaneous determination of 295 food contaminants in cereals, including 266 pesticides, 12 mycotoxins, 14 alkaloid toxins, and three Alternaria toxins [18,19,20].
Compared to food matrices, edible and medicinal plants present unique analytical challenges as dried materials containing numerous metabolites, some structurally similar to mycotoxins. This complexity creates significant matrix interference for trace mycotoxin detection, affecting methods’ sensitivity and accuracy. When applying food-based high-throughput detection methods to plants, several limitations emerge. For example, Zhao et al. observed significant matrix interference affecting mycotoxin recovery rates in nutmeg, galangal, and coix seeds using a “dilute and shoot” method [21]. When applying the QuEChERS methodology, certain aminopropyl (NH2), primary secondary amine (PSA) cleanup sorbents, while effective at removing fatty acids and organic acids, can inadvertently adsorb acid-sensitive toxins containing carboxyl groups, resulting in reduced recovery rates [21,22]. To minimize matrix interference and achieve higher recovery rates and sensitivity, combining different sample preparation methods to leverage their respective advantages presents a viable solution [23]. For instance, Nouri and Sereshti developed a rapid method combining SPE with DLLME for determining aflatoxins in soybeans [24].
Currently, research on mycotoxin contamination distribution patterns primarily focuses on food matrices and typically examines only a few specific mycotoxins or mycotoxin classes. For example, Abirami Ramu Ganesan et al. investigated the distribution patterns of Ochratoxin A and deoxynivalenol in agricultural products and related foods, while Sun et al. studied the contamination profiles of aflatoxins, ochratoxins, and fumonisins in Chinese rice [25,26]. However, there is limited research exploring the potential correlations between edible and medicinal plants and their specific mycotoxin contamination.
Chemical compounds with interactive effects may exhibit lower or higher toxic effects compared to individual substances, necessitating cumulative exposure risk assessment for multiple chemical compounds [27]. Consequently, cumulative exposure assessment methods are more appropriate for evaluating multi-mycotoxin contamination in matrices. The main cumulative exposure assessment methods include the Margin of Exposure (MOE), Hazard Index (HI), Relative Potency Factor (RPF), and Point of Departure (POD). The RPF requires similar toxicological targets, exposure routes, and duration among components in chemical mixtures, making it unsuitable for assessing contamination by diverse mycotoxin types. Additionally, there is no internationally standardized evaluation method for the POD approach. Therefore, the MOE and HI are currently the primary methods employed for cumulative mycotoxin exposure assessment. The European Food Safety Authority (EFSA) has specifically identified the MOE as the most suitable approach for evaluating genotoxic carcinogens [28]. For example, Zhang et al. applied both MOE and HI methods to assess different mycotoxins in dual-purpose plants such as coix seed and lotus seed based on their toxicity profiles [29]. Similarly, Lu et al. utilized the HI method to evaluate 31 mycotoxins in six edible and medicinal plants [30].
This study encompasses 73 mycotoxins produced by major toxigenic fungi, including Fusarium, Claviceps, Alternaria, and Penicillium species [2] (Table 1). The coverage extends to regulated mycotoxins, their associated masked forms, and emerging mycotoxins such as Enniatins and Beauvericin, aiming to provide comprehensive contamination data. Considering exposure levels, four commonly used edible and medicinal plants were selected as research subjects: lotus seed (LS), coix seed (CS) [14,31,32], licorice root (LR), commonly used as a sweetener [33], and dried tangerine peel, named “chenpi” in China (CP), often preserved as candied fruit [34]. CP can be stored for decades and its source material (tangerines) is particularly susceptible to fungal contamination.
This study developed a robust, high-throughput analytical method for these 73 mycotoxins by combining optimized QuEChERS with liquid–liquid extraction and establishing a more comprehensive ion pair library. This method was successfully applied to four edible and medicinal plants, enabling a detailed analysis of their contamination levels and characteristics. A risk assessment for Chinese populations was conducted using both the MOE and HI approaches. The findings provide valuable reference data for mycotoxin risk assessment in edible and medicinal plants and the development of relevant regulatory standards.

2. Results and Discussion

2.1. Method Optimization

2.1.1. Optimization of UHPLC-MS/MS Conditions

At the beginning of this study, mass spectrometric conditions from previous literature were referenced, including the detection of 191 mycotoxins reported by Elisabeth Varga et al. and 41 mycotoxins reported by Ann-Kristin Rausch et al. [35,36]. When applied to herbal medicine matrices, significant matrix interference was observed near some target peaks. However, this issue could be effectively resolved by modifying the MRM transitions. This demonstrates that differences in matrices require the consideration of ion pair specificity rather than merely ion response intensity. Subsequently, standard solutions of 73 mycotoxins (dissolved in 50% methanol at 500 ng/mL) were individually injected into the MS/MS system at a constant flow rate of 5 μL/min. The Analyst 1.5.1 software was used to compare and select the optimal precursor and product ions. For each mycotoxin, 3–5 ion pairs were optimized to enhance method applicability (Table 2). As shown in Figure 1, for AFB1 quantification in licorice ([M+H]+), the optimal MRM transitions were 313.0 > 241.0 and 313.0 > 269.0, while for AFB1 in tangerine peel, they were 313.0 > 241.0 and 313.0 > 285.1. Notably, although the product ion transition 313.0 > 285.1 exhibited higher intensity, undesirable interference peaks were observed near the AFB1 peak (m/z 313.0 > 285.1) in the LR. The establishment of a more comprehensive MRM transition library significantly improved the method’s versatility. To our knowledge, such an extensive ion pair spectral library for more than 70 mycotoxins has not been previously reported.
Due to the significant matrix effects in Chinese herbal medicines and the large number of target analytes, the optimized chromatographic conditions were designed to achieve maximum response intensity and optimal resolution for all analytes. Following Elisabeth Varga’s approach, chromatographic separation was performed in both positive and negative ionization modes [36]. Since more mycotoxins were separated in the positive mode, methanol (MeOH) was selected as the organic phase due to its relatively weaker elution strength, enabling better separation. Various modifiers, including formic acid, acetic acid, ammonium formate, and ammonium acetate, were evaluated to enhance ionization efficiency. The addition of 0.4% formic acid improved the response of many mycotoxins, particularly fumonisins and ochratoxins. Ammonium formate supported better peak shapes through the formation of [M+NH4]+ adducts. The optimal concentration was determined to be 2 mM, as higher concentrations (5 mM) led to ionization suppression (e.g., for ochratoxin A). In negative mode, with only 9 mycotoxins being detected, switching the organic phase from methanol to acetonitrile improved peak shapes and enhanced sensitivity without requiring modifiers. Additionally, the liquid chromatographic gradient, column temperature, and flow rate were optimized. The final mobile phases consisted of water–acetonitrile (A/B) with 0.4% formic acid and 2 mM ammonium formate for the positive mode and water–acetonitrile (A/B) for the negative mode. Based on previous research, a core-shell column (Poroshell EC-C18) was selected for its low column pressure and superior separation performance [37]. Although separating the positive and negative ionization modes sacrificed some analytical efficiency, this approach provided a better resolution when analyzing edible and medicinal plant samples, avoiding interference from matrix components and achieving a higher sensitivity. This method demonstrates broader applicability across similar matrix types.

2.1.2. Optimization of Sample Preparation

In 2006, Sulyok et al. first developed an LC-MS/MS method for multi-mycotoxin determination, using direct dilution to analyze 39 mycotoxins in cereals [17]. However, the applicability of this simplified method to plants remained uncertain. We selected LR as the model sample for preparation optimization due to its significant matrix interference. Accuracy evaluation of the “dilute and shoot” method was performed using spiked LR samples (mixed standard solution added to blank LR samples, left overnight at room temperature in a fume hood to better simulate actual mycotoxin contamination). Results indicated that the extraction solvent (acetonitrile/water/acetic acid, 79:20:1, v/v/v) was not compatible with all mycotoxins and matrix interference affected accurate quantification. Alternative extraction solvents were explored to enhance the selectivity and reduce interference, comparing extraction systems composed of formic acid, acetic acid, or citric acid buffer–acetonitrile. As no significant differences were observed among these systems, we maintained the “dilute and shoot” method’s extract solvent system for operational simplicity.
The salting-out step in QuEChERS is commonly used to remove some polar impurities, organic compounds, and proteins. To address the complexity of edible and medicinal plants, we introduced a simplified salting-out step to reduce matrix interference which proved effective in three tested plant matrices (Figure 2). Further comparison of sodium chloride, sodium acetate, and sodium citrate salt packets revealed that anhydrous sodium citrate stabilized solution pH, improving recovery rates of acid-sensitive mycotoxins by 5–8%, consistent with expectations (Figure 3). Conversely, sodium acetate decreased acidity, causing some losses of these mycotoxins.
Innovatively, unlike conventional QuEChERS, we separated the extract from the matrix before adding the aqueous solution for salting-out to minimize the co-extraction of interferents. The effects of water and 5% formic acid solution on mycotoxin recovery were investigated, with a 5% formic acid solution yielding satisfactory recovery rates (70–120%) for most of the mycotoxins.
A challenging issue arose with LS samples, which formed white precipitates during 4 °C storage after processing, affecting measurement accuracy and necessitating effective cleanup. Given the high content of starch, protein, and lipids in lotus seeds, various dispersive solid-phase extraction (d-SPE) sorbents were evaluated, including graphitized carbon black (GCB), enhanced matrix removal-lipid (EMR-Lipid), octadecyl silane (C18), aminopropyl (NH2), primary secondary amine (PSA), silica (Si), neutral aluminum oxide (Al-N), carboxymethyl (CBA), diethylaminopropyl (DEA), and cyanopropyl (CN-U). These sorbents were combined with MgSO4 (100 mg:900 mg), but none met the requirements due to their poor recovery of important mycotoxins or failure to resolve precipitation issues.
Inspired by Hyun-Deok Cho et al.’s work using hexane for preliminary lipid removal before immunoaffinity column cleanup [22], we modified the approach using cyclohexane. Adding 12.0 mL cyclohexane to 6.0 mL extract significantly improved the precipitation issue while minimally affecting mycotoxin recovery, with only 1–2 mycotoxins showing losses around 7.8% and others below 1.8%. Ultimately, liquid–liquid extraction with cyclohexane was adopted as the cleanup method.

2.2. Method Validation

Method validation was performed on three different edible and medicinal plants (LS, LR, and CP), evaluating key analytical parameters including the linearity, accuracy, limits of detection (LOD), limits of quantification (LOQ), and precision. The comprehensive validation data are summarized in Table A1, Table A2 and Table A3.

2.2.1. Linearity

Due to matrix effects exceeding ±20% for most mycotoxins, matrix-matched calibration curves were necessary for accurate quantification. Blank sample extracts after nitrogen evaporation were reconstituted with 0.5 mL acetonitrile, followed by the addition of varying amounts of mixed standard stock solutions. The solutions were then made up to 2 mL with solvent (acetonitrile/water/acetic acid, 20:79:1). Three concentration ranges were prepared: G1 (0.1, 0.5, 1, 5, 10, 20, and 50 ng/mL), G2 (0.5, 2.5, 5, 25, 50, 100, and 250 ng/mL), and G3 (2.5, 12.5, 25, 125, 250, 500, and 1250 ng/mL). Calibration curves were constructed using peak area versus concentration relationships. All mycotoxins demonstrated good linearity with correlation coefficients (r) greater than 0.998.

2.2.2. Method Limit of Quantification (LOQ) and Limit of Detection (LOD)

Spiking experiments were conducted to determine the method’s quantification limits (LOQs) for each matrix. At spiking levels of 0.5 μg/kg (calculated as AFB1), LS and CP samples met requirements for signal-to-noise ratio, recovery, and precision. However, LR samples required a higher LOQ of 1.0 μg/kg (calculated as AFB1), which better reflected actual sample conditions. The LOQs for the three matrices ranged from 0.5 to 25.0 µg/kg, as shown in Table A1, Table A2 and Table A3. Despite using generic extraction and cleanup procedures, the method achieved lower LOQs for several mycotoxins compared to existing reports [29,38]. The LOQs were significantly below the maximum residue limits (MRLs) set by Commission Regulation (EU) No 2023/915. For example, the LOQ for FB1 and FB2 was 2.5 µg/kg, well below the MRL of 200 µg/kg, demonstrating the method’s suitability for regulatory monitoring of these edible and medicinal plants. Limits of detection (LODs) were determined at spiking levels of 0.25 μg/kg (calculated as AFB1) for LS and 0.5 μg/kg (calculated as AFB1) for CP and LR.
During the method’s development, matrix interference for certain mycotoxins in several plants remained unresolved. Consequently, some mycotoxins, such as tenuazonic acid, were excluded from the final method and require further optimization.

2.2.3. Method Accuracy and Precision

In the absence of certified reference materials, the method’s accuracy was evaluated using recovery rates (obtained by spiking known amounts of analytes into blank matrices). Recovery studies were performed at three concentration levels in three blank matrices (n = 6): 1.0 μg/kg (Level 1), 5.0 μg/kg (Level 2), and 10.0 μg/kg (Level 3) (calculated as AFB1). The recovery rates for the 73 target analytes ranged from 61.6% to 116.4%, with RSDs less than 14.9%. These results largely comply with European Commission Regulation (EC) No 401/2006, indicating the satisfactory accuracy and precision of the method.

2.3. Mycotoxin Contamination of Edible and Medicinal Plants

The established analytical method was applied to analyze 260 batches of four different edible and medicinal plants to characterize their mycotoxin contamination patterns and summarize the distinct contamination characteristics across different plants.

2.3.1. Lotus Seed (LS)

Lotus seeds have a 7000-year history as a vegetable, functional food, and medicinal herb. China is the world’s largest lotus root cultivator and consumer, with a cultivation area of 200,000 hectares [31]. By 2017, Fujian Province’s annual lotus seed production reached 12,205 tons, contributing approximately 1.8 billion RMB to the country’s GDP [39].
This study analyzed twenty-nine LS samples, including nine special samples (LS28-36): fresh powder (LS31), moldy powder (LS35), discolored powder (LS36), three farm-cultivated powders (LS32-34), and three commercial medicinal samples (LS28-30). In total, 17 mycotoxins were detected in the samples (Table A4), with an 86.2% detection rate, primarily produced by Aspergillus species (Figure 4 and Figure 5). Notably, LS showed the highest aflatoxin contamination rate (41.4%) among the four studied plants, at 34.5%, exceeding the Chinese Pharmacopoeia (Ch.P) limits. Three samples—the moldy, discolored, and one farm-cultivated sample—contained AFB1 levels up to 4000 μg/kg (Figure 6), indicating rapid aflatoxin accumulation in deteriorated lotus seeds to alarming levels.
The data revealed that highly aflatoxin-contaminated samples frequently contained related metabolites such as AFM1, AFM2, Ster, and O-m-ster. Interestingly, AFM1 and AFM2, previously reported only in milk as AFB1 metabolites in animals, had never been detected in herbs and spices [40], suggesting possible non-animal AFB1 metabolism pathways worthy of further investigation. Additionally, CPA levels exceeded 10,000 μg/kg in these samples, confirming previous reports of AF-CPA co-occurrence and increased toxicity risks [41,42]. Research suggests CPA may serve as a fungal colonization signal molecule, related to its calcium ion-inhibitory activity [43]. Our finding of CPA as the sole mycotoxin in fresh lotus seeds partially supports this hypothesis.
The significance analysis of mycotoxin contamination in LS of different forms was conducted using the Mann–Whitney U test. Statistical analysis revealed significantly higher detection rates of AFB1, AFB2, AFM1, AFM2, CIT, CPA, and O-m-Ster in powder form compared to the original form (p < 0.05). No significant differences were observed in the contamination levels of other mycotoxins between the two forms (Figure 7 and Figure 8). The average increase in detection rates was calculated to be 24.7%. Additionally, only discolored lotus seeds contained CIT, a nephrotoxic mycotoxin produced by Aspergillus, Penicillium, or related fungi, possibly explaining the color change. Therefore, appearance may serve as an important quality indicator for lotus seeds.

2.3.2. Licorice Root (LR)

LR, derived from the dried roots and rhizomes of Glycyrrhiza uralensis Fisch, G. glabra L., or G. inflata Bat., appears in approximately 60% of TCM prescriptions due to its complementary properties [37]. As one of China’s most widely used herbs, LR is included in multiple pharmacopeias, including Chinese, Korean, European, and United States Pharmacopeias, due to its widespread global use for its sweet taste.
Among 77 samples, 18 mycotoxins were detected, with an overall detection rate of 59.7% (Table A5). The concerning OTA showed a low detection rate of 3.9%, with no samples exceeding the European Pharmacopoeia 11.0 limit (20 μg/kg), while ZEN was detected in 15.6% of samples, indicating potential risks. Contrary to previous reports of high OTA occurrence in licorice [44], 47 samples (LR31-77) from five Chinese regions (Xinjiang, Inner Mongolia, Gansu, Jilin, and Ningxia) showed no OTA contamination, suggesting a possible geographical difference between European and Chinese cultivation regions. Using the same Mann–Whitney U test, sliced LR showed higher contamination levels and detection rates of regulated mycotoxins (FB1, FB2, OTA, MPA, Pse A, CIT, and AME) compared to raw materials (p < 0.05), with increases of 3.7% to 42.1% (Figure 7 and Figure 8).
ENNs and BEA were the predominant contaminants in LR, typically co-occurring due to their similar chemical structures produced by Fusarium species. The four enniatins consistently showed a concentration pattern of ENN B > ENN B1 > ENN A1 > ENN A. Research suggests that different Fusarium species preferentially incorporate specific amino acids to biosynthesize certain ENNs, explaining why some ENNs can only be isolated from specific fungal strains [45]. The consistent concentration pattern observed in this study suggests contamination by a single Fusarium species.
This study analyzed 47 batches of GR samples (GR31-77) that were collected and processed between 2015 and 2020. All samples were maintained in a temperature-controlled storage facility (≤20 °C). Statistical analysis using the Kruskal–Wallis test (α = 0.05) revealed no significant temporal differences in mycotoxin levels, with the exception of beauvericin (BEA), enniatin A (ENN A), and enniatin A1 (ENN A1). This indicates that strict collection and storage management with controlled environmental conditions effectively reduces mycotoxin contamination in LR.

2.3.3. Dried Tangerine Peel (CP)

CP, derived from mature fruit peels of Citrus reticulata Blanco and its cultivars, is not only one of the most renowned TCMs but also serves as an ingredient in fermented foods [34]. Studies suggest that the quality improves with storage duration [46]. Given its extended storage requirements–typically over three years before use as a medicinal herb–CP faces potential mycotoxin contamination risks. However, multi-mycotoxin contamination in CP has not been extensively documented.
Surprisingly, CP showed the lowest contamination risk among the four matrices studied. Only five mycotoxins were detected in 131 samples, with a detection rate of 38.2%, primarily from Penicillium species (Figure 4) (Table A6). Consistent with previous research, MPA showed the highest detection rate (35.9%), mainly produced by Penicillium [47], confirming citrus fruits’ susceptibility to Penicillium contamination [48]. Similar to LR, BEA-positive samples showed concurrent ENN detection, following the same contamination pattern of ENN B > ENN B1 > ENN A1, suggesting possible contamination by the same Fusarium species. However, our findings differ from previous studies on fresh citrus peel fungal communities, which identified Erythrobasidium, Penicillium, Aspergillus, Rhodotorula, and Mycosphaerella as the dominant genera, with the rare detection of Fusarium [49]. The low levels of BEA and ENNs in CP suggest initial field contamination by multiple fungi including Fusarium, Penicillium, and Aspergillus, with Fusarium gradually being replaced by other dominant fungi.

2.3.4. Coix Seed (CS)

CS is a widely used medicinal and edible grain that has gained popularity as a health food, especially among women, for its properties in eliminating dampness and reducing swelling. It is increasingly consumed as a daily beverage alternative to coffee.
Among 47 samples, 27 mycotoxins were detected, with a total detection rate of 89.4% (Table A7). The analysis of mycotoxin-producing fungi revealed that CS was most susceptible to Aspergillus and Fusarium contamination (Figure 4). Fusarium mycotoxins showed the highest detection rates, with FBs (2.9–430.7 μg/kg) at 74.4% and ZEN (4.1–206.9 μg/kg) at 59.6%. Additionally, AFs showed a significant detection rate of 27.7%, validating the necessity of aflatoxin and zearalenone limits in coix seeds as specified in the Ch.P.
Consistent with previous literature reports, CS showed significant multi-mycotoxin contamination, including both parent mycotoxins and their modified forms [12]. The most severely contaminated sample contained 17 different mycotoxins, with over 50% of samples containing at least four mycotoxins (Figure 5). Samples with high levels of parent mycotoxins often showed a concurrent detection of their modified forms. For example, sample CS3 contained ZEN along with ZAN, α-ZEL, β-ZEL, and α-ZAL, while sample CS6 showed both DON and 3-Ac-DON. Modified mycotoxins showed lower detection rates and contamination levels compared to their parent compounds.
Interestingly, ENNs, which were common contaminants in the other three matrices and are produced by Fusarium, were not detected in CS samples. This might be related to differences in the dominant fungal species colonizing CS, suggesting possible competitive relationships among fungi.

2.4. Health Risk Assessment

A health risk assessment was performed according to the guidelines established by the International Agency for Research on Cancer (IARC) and the Joint FAO/WHO Expert Committee on Food Additives (JECFA), evaluating mycotoxins that showed detection frequencies above 20% in the studied matrices.

2.4.1. Estimation of Exposure

The exposure to mycotoxins was calculated using the average contamination levels from 260 batches of four matrices, average body weight, and daily intake doses. Daily intake doses were based on the maximum recommended dosages specified in the 2020 edition of the Ch.P, with maximum daily intakes set at 15 g for LS, 10 g for LR, 10 g for CP, and 30 g for CS. The average body weight of 64.4 kg was derived from the “Report on Nutrition and Chronic Diseases of Chinese Residents (2020)”, accounting for male-to-female population ratios. Mycotoxin exposure was calculated using the following formula, with detailed exposure levels presented in Table 3.
Exposure = (C × IR)/BW
C represents the average mycotoxin contamination level in medicinal materials (ng/g); IR represents the daily intake rate (g·day−1); and BW represents the average body weight (kg). Following the principles for handling non-detect data from the WHO Global Environment Monitoring System/Food Contamination Monitoring and Assessment Programme (GEMS/Food) Second Workshop on “Reliable Evaluation of Low-Level Contamination of Food” and the standards proposed in the European Commission’s Scientific Cooperation Task 3.2.10 (SCOOP) [50], the contamination level was calculated as the mean of all samples, with non-detect samples assigned a value of 1/2 LOD.

2.4.2. Risk Assessment of Mycotoxins Based on Margin of Exposure Margin of Exposure (MOE)

For non-threshold carcinogenic chemical hazards such as aflatoxins, risk assessment was conducted using the MOE approach, based on the Benchmark Dose Lower confidence limit of 10% extra risk (BMDL10) parameters published by EFSA (see Table 3) [51]. An MOE value greater than 10,000 indicates an acceptable health risk, while values below this threshold suggest potential health concerns.
MOE = BMDL10/Exposure
MOE values were calculated for seven mycotoxins (AFB1, AFB2, AFG1, AFG2, AFM1, AFM2, and Ster), as shown in Figure 9. CP and LR were excluded from the analysis due to the non-detection of the relevant mycotoxins. The analysis revealed that Ster posed minimal risks in both LS and CS for its lower toxicity. However, the MOE values for the remaining six aflatoxins were all below 10,000, indicating potential health concerns. While AFM2 showed values approaching 10,000, the actual risk might be higher considering that LS, as both medicinal and food items, may be consumed in larger quantities than the calculated dose. Additionally, children with lower body weights may face elevated risks. These findings highlight the necessity for monitoring aflatoxin risks in LS and CS.

2.4.3. Risk Assessment of Mycotoxins Based on Hazard Index (HI)

For threshold hazardous compounds such as DON and ZEN, a risk assessment was conducted using the HI method, based on the Provisional Maximum Tolerable Daily Intake (PMTDI) [52]. The HI is calculated as the sum of Hazard Quotients (HQ) for individual chemical compounds. An HI value less than 1 indicates an acceptable exposure risk level, while values exceeding 1 suggest potential adverse effects on human health. For certain mycotoxins with insufficient toxicological data and no official PMTDI values, a reference value of 2000 ng·kg−1 b.w.·day−1 was adopted (including BEA, CPA, ENNs, and MPA), based on fumonisin toxicity data.
HQ = Exposure/PMTDI
HI = ∑HQ
As shown in Figure 10, the overall HI values for all four matrices were relatively low, with ZEN and CIT being the primary risk contributors. Among these, CPA was the largest contributor to the HI in LS, with a value of 0.23. Although CS showed low overall risk, the high detection rate of fumonisins (74.4%) suggests potential exposure risks, indicating the need for expanded data collection to comprehensively evaluate the necessity of including these compounds in regulatory standards.

3. Conclusions

This study systematically revealed the mycotoxin contamination characteristics and potential risks in four dual-use medicinal and edible plants through contamination analysis and health risk assessment.
Contamination analysis demonstrated that different types of plants exhibited unique contamination profiles: seed-type plants (LS and CS) were susceptible to Aspergillus and Fusarium contamination, with detection rates of 53.3% and 22.2%, respectively. Despite low detection rates, the MOE values of six aflatoxins remained below 10,000 due to their high toxicity, indicating potential health risks. Peel-type materials (citrus peel) were primarily contaminated with MPA produced by Penicillium (35.9% detection rate), while root-type materials (licorice) were mainly affected by Fusarium species (52.9% detection rate).
Significant differences were observed in contamination levels between samples of different physical states, with fragmented samples showing more severe mycotoxin contamination. Powdered LS showed a 52.9% higher detection rate of AFB1 compared to whole seeds, while sliced LR demonstrated a 41.2% higher detection rate of FB1 than intact samples. Three highly positive lotus samples, including moldy samples, discolored samples, and farm-cultivated samples, although limited in number, suggested the importance of timely drying and standardized sourcing in preventing mycotoxin contamination. GR samples collected between 2015 and 2020 and stored under cool conditions showed no significant differences in mycotoxin contamination.
Furthermore, highly contaminated samples revealed the co-occurrence of metabolically related toxins, such as the simultaneous detection of AFM1 (2.1 μg/kg) and AFM2 (1.8 μg/kg) in lotus seeds, providing new directions for studies on the metabolic mechanisms of mycotoxins in plants. Interestingly, the CP samples included in this study showed very low exposure risk (HI = 0.07%), possibly attributed to their specific processing techniques (e.g., low-temperature drying) or natural antimicrobial components.
The multi-toxin detection method established in this study can be extended to other plants with similar compositions, providing important technical support and data references for establishing scientific quality control systems. Based on our findings, we propose the following recommendations for industrial applications: (1) Emphasize supplier qualification verification during raw material procurement and strictly control moisture content; (2) Process and store materials in a non-fragmented state when possible and use sealed packaging after drying to prevent moisture absorption; (3) Maintain storage temperatures below 20 °C during storage and transportation and utilize dehumidification equipment when possible.

4. Materials and Methods

4.1. Sample Collection

A total of 260 samples of four edible and medicinal plants were collected, including LR (77 batches), LS (29 batches), CS (47 batches), and CP (131 batches). The majority of samples were obtained through national or local quality surveillance programs from various provinces in China, with a few collected directly from local farmers. All samples were authenticated by Chief Pharmacist Yang Xinhua from the Traditional Chinese Medicine/Natural Medicine and Health Food Institute, Shanghai Institute of Food and Drug Control. For each matrix, 200 g of sample was collected, ground into fine powder, passed through a 50-mesh sieve, and stored at −20 °C.

4.2. Chemicals and Reagents

Acetonitrile and methanol were purchased from Merck (Darmstadt, Germany). LC-MS grade acetic acid and formic acid were supplied by Fisher Scientific (Somerville, USA). Ammonium formate and ammonium acetate were obtained from Sigma Aldrich (Zwijndrecht, The Netherlands). Analytically pure acetic acid, formic acid, ammonium formate, and ammonium acetate were purchased from Merck (Darmstadt, Germany). Deionized water was obtained using a Milli-Q Gradient Water System (Millipore, Bedford, MA, USA).
Anhydrous magnesium sulfate, sodium chloride, trisodium citrate dihydrate, disodium citrate hydrate, anhydrous sodium acetate, dispersed solid-phase extraction (d-SPE) sorbent octadecylsilane (C18), primary secondary amine (PSA), silica gel (Si), and propane sulfonic acid (PRS) were obtained from Bonna-Agela Technologies (Tianjin, China). Solid reagent anhydrous sodium acetate prepared for buffer solution was obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All other reagents were of analytical grade.
Solid standards or stock solutions were collected from various sources (Table A8) and the information on the 73 mycotoxins’ standards is listed in Table 1. The declared purities of all standards ranged from 92.87% to 99.9%.

4.3. Preparation of Standard Solution

Stock solutions of mycotoxins were prepared in acetonitrile at concentrations ranging from 10 to 250 μg/mL and stored in brown glass vials at −20 °C, respectively. Based on their mass spectrometric response intensities, the 73 mycotoxins were divided into three groups. Group 1 (G1) included the following mycotoxins: 7-dechloro griseofulvin, aflatoxin B1, B2, G1, G2, and M1, agroclavine, anisomycin, beauvericin, diacetoxyscirpenol, dihydrolysergamide, enniatin A, enniatin A1, enniatin B, enniatin B1, ergocornine, ergocorninine, ergocristine, ergocristinine, ergocryptine, ergocryptinine, ergosine, griseofulvin, lysergamide, meleagrin, mycophenolic acid, ochratoxin A, ochratoxin B, ochratoxin C, oxaline, puromycin, roquefortine C, and sterigmatocystin. Group 2 (G2) included 15-acetoxyscirpenol, aflatoxin M2, aflatoxin P1, apicidin, chaetocin, citrinin, cyclopiazonic acid, equisetin, fumonisin B1, fumonisin B2, fumonisin B3, gliotoxin, monocerin, neosolaniol, o-methylsterigmatocystin, pseurotin A, secalonic acid D, T-2 toxin, α-zearalanol, α-zearalenol, β-zearalanol, β-zearalenol, and zearalenone. Group 3 (G3) included 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, chetomin, citreoviridin, deepoxy-deoxynivalenol, deoxynivalenol, fumagillin, fusarenon X, HT-2 toxin, Ostreogrycin A, T-2-triol, tentoxin, wortmannin, patulin, alternariol-methylether, and alternariol.
Mixed standard stock solutions were prepared by combining individual stock solutions from each group to achieve the following concentrations: 100 ppb for G1, 500 ppb for G2, and 2500 ppb for G3. Working standard solutions at various concentration levels were subsequently prepared by appropriate dilution with suitable solvents as required by the analytical method.

4.4. Sample Preparation

An accurately weighed 2.0 g portion of homogenized sample was transferred into a 50 mL polypropylene centrifuge tube. Extraction was carried out with 20 mL of acetonitrile–water–acetic acid (80:19:1, v/v/v) using an orbital shaker (IKA, Guangzhou, China) for 90 min, followed by centrifugation (Eppendorf, Hamburg, Germany) at 3900 rpm for 5 min. Subsequently, 10 mL of the supernatant was transferred and combined with 10 mL of 5% formic acid solution. A QuEChERS salt mixture (sodium chloride, anhydrous magnesium sulfate, sodium citrate, and sodium citrate sesquihydrate; 1 g:4 g:1 g:0.5 g) was added immediately, followed by high-speed vortexing for 5 min (SPEX, New York, NY, USA) and centrifugation at 3900 rpm for 5 min. For further purification, 6.0 mL of the supernatant was subjected to liquid–liquid partitioning with 12 mL cyclohexane, followed by centrifugation at 3900 rpm. The lower phase (4 mL) was collected and concentrated to near dryness under a gentle nitrogen stream at 40 °C. The residue was reconstituted in 0.5 mL acetonitrile and diluted to 2 mL with water. The final extract was filtered through a 0.22 μm PTFE membrane filter (Agilent, Shanghai, China) prior to UHPLC-MS/MS analysis, with an injection volume of 5 μL.

4.5. UHPLC-MS/MS Analysis

Chromatographic separation was performed on a 1290 UHPLC system equipped with a quaternary solvent delivery system, degasser, autosampler, and column thermostat, coupled to a 5500 triple quadrupole mass spectrometer (AB SCIEX, Framingham, MA, USA) with an electrospray ionization (ESI) source operating in both positive and negative modes.
Chromatographic separation of the 73 mycotoxins was achieved on a Poroshell EC-C18 column (150 × 3.0 mm, 2.7 μm) (Agilent, Wilmington, DE, USA) at a flow rate of 450 μL/min. For the 64 mycotoxins analyzed in the positive mode (ESI+), mobile phase A consisted of 0.4% formic acid and 2.0 mM ammonium formate in water and mobile phase B consisted of 0.4% formic acid and 2.0 mM ammonium formate in methanol. The gradient program was 0–2 min, 20% B; 2–6 min, 20–50% B; 6–11 min, 50–55% B; 11–15 min, 55–100% B; 15–19 min, 100% B; 19–21 min, 100–20% B; and 21–25 min, 100% B. For the remaining mycotoxins analyzed in negative mode (ESI−), mobile phase A was water and mobile phase B was acetonitrile, with the following gradient: 0–2 min, 10% B; 2–8 min, 10–50% B; 8–13 min, 50–60% B; 13–15 min, 60–100% B; 15–16 min, 100% B; 16–18 min, 100–10% B; and 18–20 min, 10% B. The injection volume was 1.0 μL, the column temperature was maintained at 35 °C, and the sample tray temperature was set at 15 °C to enhance sample stability.
Mass spectrometric detection was performed under the following conditions: for the positive mode, the ion spray voltage was 5.5 kV, curtain gas was 30 psi, ion source gas 1 and gas 2 were both 50 psi, and source temperature was 450 °C; for the negative mode, the ion spray voltage was 4.5 kV, curtain gas was 30 psi, ion source gas 1 and gas 2 were both 50 psi, and source temperature was 400 °C.
A multiple reaction monitoring (MRM) mode was employed, with at least one precursor ion and two product ions monitored for each mycotoxin. The two most intense product ions free from matrix interference were selected for quantification and qualification, respectively. Declustering potentials (DPs) and collision energies (CEs) were optimized individually using standard solutions for each analyte.
Data acquisition and processing were performed using Analyst 1.5.1 and MultiQuant™ 2.1.1 software (AB SCIEX). Detailed information regarding the retention times (RTs), monitored precursor and product ions, and optimized DPs and CEs for each mycotoxin is presented in Table 1.

4.6. Statistical Analysis

All statistical analyses were performed using R software (version 4.2.0; R Core Team, 2023). Due to the non-normal distribution of data and presence of numerous null values, non-parametric analyses were conducted using the “stats” package. The wilcox.test() function was employed for Mann–Whitney U tests to evaluate differences in mycotoxin contamination between different forms of samples, while the kruskal.test() function was used for Kruskal–Wallis tests to analyze differences among different harvest years. Data visualization was accomplished using the “ggplot2” package. Statistical significance was defined as p < 0.05.

Author Contributions

Conceptualization, Investigation, Writing—Original draft preparation: X.H.; Validation, Data Curation: R.F.; Visualization: Q.H.; Reviewing and Editing: X.M.; Supervision, Project administration: H.Z.; All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Natural Science Foundation of Shanghai (23ZR1457200), Shanghai Science and Technology Commission R&D Platform Program (21DZ2290200), and Shanghai Municipal Administration of Market Regulation Technical Support Project (2024BZ20).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The original contributions presented in this study are included in the article. Further inquiries can be directed to the corresponding author(s).

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A

Table A1. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in LS.
Table A1. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in LS.
MycotoxinSpiked Levels (μg/kg)LOD
(μg/kg)		LOQ
(μg/kg)		Linear Range
(μg/kg)		Coefficient (r)
Level 1Level 2Level 3
R
(%)		RSD
(%)		R
(%)		RSD
(%)		R
(%)		RSD
(%)
15-Asp88.83.186.42.383.13.51.252.52~10000.99973
15-ADON81.95.282.24.279.84.16.2512.510~50000.99990
3-ADON90.82.789.12.288.03.56.2512.510~50000.99973
7-D-G94.91.097.02.294.93.70.250.50.4~2000.99959
AFB191.52.092.32.886.13.40.250.50.4~2000.99967
AFB292.41.492.52.288.32.20.250.50.4~2000.99995
AFG191.11.490.54.283.80.90.250.50.4~2000.99993
AFG287.21.983.43.882.24.80.250.50.4~2000.99985
AFM186.83.389.13.087.42.20.250.50.4~2000.99925
AFM289.22.189.83.985.63.61.252.52~10000.99929
AFP190.02.482.12.084.97.51.252.52~10000.99993
Agro82.82.587.63.986.22.40.250.50.4~2000.99964
Anis96.42.7102.52.7103.31.90.250.50.4~2000.99936
Apici80.43.281.92.879.23.51.252.52~10000.99988
BEA63.55.969.56.769.411.40.250.50.4~2000.99985
Chae82.99.691.36.888.98.46.2512.510~50000.99928
Che85.99.085.73.781.33.91.252.52~10000.99986
CIT75.62.672.93.073.44.41.252.52~10000.99991
CVD81.34.784.22.885.04.26.2512.510~50000.99987
CPA69.15.073.13.177.18.91.252.52~10000.99964
DAS84.34.688.23.089.93.40.250.50.4~2000.99928
DiLy72.76.585.01.688.23.70.250.50.4~2000.99942
DOM92.52.489.68.287.06.56.2512.510~50000.99977
DON89.01.489.33.689.32.56.2512.510~50000.99978
ENN A76.03.571.85.072.18.70.250.50.4~2000.99985
ENN A173.64.678.13.478.06.30.250.50.4~2000.99984
ENN B80.13.582.32.483.15.10.250.50.4~2000.99964
ENN B181.32.083.93.288.05.00.250.50.4~2000.99990
Equi71.42.071.51.777.26.91.252.52~10000.99957
EGCN82.02.887.91.885.03.00.250.50.4~2000.99957
EGCNN93.54.0100.03.291.83.00.250.50.4~2000.99984
EGST101.41.9103.73.9103.24.90.250.50.4~2000.99977
EGSTN81.42.282.73.487.23.00.250.50.4~2000.99983
EGPT79.51.879.73.679.93.90.250.50.4~2000.99955
EGPTN90.62.191.62.689.63.30.250.50.4~2000.99979
EGSN116.43.1113.23.2106.14.70.250.50.4~2000.99928
FB176.42.677.41.775.43.01.252.52~10000.99938
FB273.43.576.53.574.75.01.252.52~10000.99905
FB374.37.181.05.477.05.11.252.52~10000.99951
Fum84.012.875.65.879.12.66.2512.510~50000.99933
FuX87.82.386.35.182.72.86.2512.510~50000.99978
Glio83.91.682.33.981.93.41.252.52~10000.99920
Grise90.51.290.02.385.33.60.250.50.4~2000.99950
HT-288.31.784.92.582.53.46.2512.510~50000.99978
Lyser76.32.280.22.078.53.60.250.50.4~2000.99959
Melea83.81.983.73.980.62.80.250.50.4~2000.99945
Mono95.10.798.83.295.33.51.252.52~10000.99997
MPA114.73.0109.32.797.93.20.250.50.4~2000.99997
NEO78.53.877.52.576.13.91.252.52~10000.99979
O-m-Ster92.30.999.93.393.63.31.252.52~10000.99961
Ostre A95.51.8103.92.398.32.86.2512.510~50000.99941
OTA82.54.785.24.483.310.20.250.50.4~2000.99927
OTB88.21.995.01.790.94.00.250.50.4~2000.99972
OTC80.66.279.42.179.82.60.250.50.4~2000.99981
Oxa83.34.788.44.283.63.30.250.50.4~2000.99944
Pse A95.54.2101.03.697.72.11.252.52~10000.99927
Puro72.82.678.94.980.95.50.250.50.4~2000.99959
Rq C78.82.174.52.578.03.80.250.50.4~2000.99966
Secal A75.45.388.73.678.26.21.252.52~10000.99944
Ster90.06.388.70.481.04.10.250.50.4~2000.99963
T2-triol83.52.882.92.879.33.16.2512.510~50000.99946
T-293.93.994.73.792.73.11.252.52~10000.99930
Ten96.72.1110.52.1109.23.16.2512.510~50000.99913
Wor-man92.93.899.63.593.03.76.2512.510~50000.99999
α-ZAL88.82.787.02.288.03.01.252.52~4000.99939
α-ZEL89.23.583.71.281.81.91.252.52~4000.99955
β-ZAL94.04.288.22.779.63.11.252.52~10000.99978
β-ZEL87.24.684.52.883.42.61.252.52~10000.99970
PAT85.02.780.53.678.02.46.2512.510~50000.99946
ZAN92.63.283.64.472.32.51.252.52~10000.99948
ZEN94.94.880.82.183.54.21.252.52~10000.99985
AME91.91.794.32.494.52.96.2512.510~20000.99952
AOH87.05.777.74.781.63.76.2512.510~20000.99931
Table A2. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in GR.
Table A2. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in GR.
MycotoxinSpiked Levels (μg/kg)LOD
(μg/kg)		LOQ
(μg/kg)		Linear Range
(μg/kg)		Coefficient (r)
Level 1Level 2Level 3
R
(%)		RSD
(%)		R
(%)		RSD
(%)		R
(%)		RSD
(%)
15-Asp84.65.279.74.579.64.12.55.02~10000.99950
15-ADON83.66.183.62.481.76.912.525.010~50000.99956
3-ADON88.55.381.53.278.33.112.525.010~50000.99987
7-D-G84.02.781.23.279.92.40.51.00.4~2000.99916
AFB195.98.083.39.983.66.40.51.00.4~2000.99924
AFB2109.67.282.54.279.74.50.51.00.4~2000.99957
AFG193.75.975.84.577.37.60.51.00.4~2000.99946
AFG287.513.288.39.088.87.70.51.00.4~2000.99990
AFM194.46.184.35.587.03.50.51.00.4~2000.99969
AFM292.23.580.94.384.84.32.55.02~10000.99924
AFP194.77.174.04.572.76.12.55.02~10000.99959
Agro78.97.372.96.273.35.00.51.00.4~2000.99901
Anis86.04.487.44.787.63.30.51.00.4~2000.99962
Apici84.97.875.25.777.16.62.55.02~10000.99919
BEA91.25.484.31.781.64.30.51.00.4~2000.99920
Chae83.310.275.29.478.16.512.525.010~50000.99959
Che83.210.679.56.479.58.52.55.02~10000.99899
CIT70.63.572.44.374.15.32.55.02~10000.99963
CVD86.114.278.47.876.58.012.525.010~50000.99989
CPA73.611.867.68.864.34.72.55.02~10000.99982
DAS83.33.079.83.178.04.10.51.00.4~2000.99968
DiLy62.72.567.43.565.94.90.51.00.4~2000.99939
DOM85.22.682.13.276.82.312.525.010~50000.99948
DON75.44.676.73.474.93.412.525.010~50000.99981
ENN A86.35.082.02.779.32.70.51.00.4~2000.99920
ENN A187.53.682.93.879.34.10.51.00.4~2000.99943
ENN B92.14.983.63.180.34.30.51.00.4~2000.99976
ENN B185.94.181.54.379.92.80.51.00.4~2000.99930
Equi80.47.573.14.369.24.12.55.02~10000.99892
EGCN94.24.673.65.278.04.50.51.00.4~2000.99928
EGCNN88.43.280.63.177.45.20.51.00.4~2000.99980
EGST102.86.886.04.180.63.40.51.00.4~2000.99946
EGSTN87.54.675.13.071.03.20.51.00.4~2000.99912
EGPT95.86.377.53.970.62.90.51.00.4~2000.99936
EGPTN85.26.576.84.479.86.10.51.00.4~2000.99911
EGSN94.77.691.34.294.06.20.51.00.4~2000.99927
FB174.57.576.67.979.68.62.55.02~10000.99951
FB271.711.477.99.487.411.12.55.02~10000.99935
FB379.211.186.69.292.24.82.55.02~10000.99945
Fum81.67.982.29.0105.410.312.525.010~50000.99945
FuX84.64.880.32.777.02.712.525.010~50000.99958
Glio80.73.082.63.478.44.22.55.02~10000.99907
Grise69.87.072.29.280.54.40.51.00.4~2000.99917
HT-287.84.983.73.982.14.212.525.010~50000.99949
Lyser71.34.767.15.068.43.80.51.00.4~2000.99998
Melea87.92.280.83.876.12.90.51.00.4~2000.99923
Mono83.73.779.44.476.71.62.55.02~10000.99972
MPA82.75.487.46.684.23.10.51.00.4~2000.99894
NEO87.11.982.85.477.22.52.55.02~10000.99909
O-m-Ster77.94.870.37.472.914.42.55.02~10000.99947
Ostre A86.75.575.96.176.69.112.525.010~50000.99906
OTA83.26.877.76.380.57.30.51.00.4~2000.99904
OTB87.24.585.83.482.42.00.51.00.4~2000.99940
OTC98.56.180.54.277.55.20.51.00.4~2000.99984
Oxa85.12.881.84.578.83.50.51.00.4~2000.99952
Pse A86.85.483.84.282.45.02.55.02~10000.99925
Puro72.29.169.97.775.64.10.51.00.4~2000.99917
Rq C78.37.473.312.773.514.90.51.00.4~2000.99989
Secal A86.713.278.69.474.27.52.55.02~10000.99928
Ster80.910.870.84.870.514.60.51.00.4~2000.99966
T2-triol93.82.685.74.680.33.312.525.010~50000.99914
T-291.65.086.01.681.13.52.55.02~10000.99975
Ten80.87.286.04.088.12.112.525.010~50000.99995
Wor-man88.72.882.92.880.84.112.525.010~50000.99984
α-ZAL85.510.272.06.475.27.62.55.02~4000.99973
α-ZEL83.94.372.95.576.84.82.55.02~4000.99908
β-ZAL76.58.078.410.082.85.52.55.02~10000.99960
β-ZEL82.510.294.84.198.613.32.55.02~10000.99897
PAT77.98.376.77.678.99.812.525.010~50000.99957
ZAN83.47.678.34.677.62.52.55.02~10000.99998
ZEN90.26.980.93.476.54.72.55.02~10000.99965
AME98.84.193.42.189.72.512.525.010~10000.99869
AOH106.96.793.12.581.64.712.525.010~10000.99849
Table A3. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in CP.
Table A3. Overview of the extraction recovery (R), repeatability (RSD), limit of detection (LOD), and limit of quantification (LOQ) for each mycotoxin in CP.
MycotoxinSpiked Levels (μg/kg)LOD
(μg/kg)		LOQ
(μg/kg)		Linear Range
(μg/kg)		Coefficient (r)
Level 1Level 2Level 3
R
(%)		RSD
(%)		R
(%)		RSD
(%)		R
(%)		RSD
(%)
15-Asp82.66.382.91.778.53.01.252.52~10000.99917
15-ADON87.84.479.53.873.85.36.2512.510~50000.99981
3-ADON87.74.783.53.279.92.56.2512.510~50000.99934
7-D-G79.06.083.83.480.62.50.250.50.4~2000.99981
AFB181.65.484.23.278.52.60.250.50.4~2000.99911
AFB278.55.583.72.778.43.40.250.50.4~2000.99923
AFG181.76.683.63.080.63.00.250.50.4~2000.99980
AFG283.16.483.62.083.33.70.250.50.4~2000.99929
AFM181.94.083.44.578.93.70.250.50.4~2000.99979
AFM287.38.682.65.975.24.81.252.52~10000.99975
AFP180.36.581.94.777.44.01.252.52~10000.99912
Agro80.34.880.24.275.34.60.250.50.4~2000.99985
Anis82.37.081.34.077.64.60.250.50.4~2000.99902
Apici82.34.783.92.079.03.61.252.52~10000.99904
BEA79.26.881.72.479.32.90.250.50.4~2000.99926
Chae86.39.883.76.679.96.16.2512.510~50000.99957
Che82.08.682.34.680.44.81.252.52~10000.99928
CIT77.36.479.27.574.38.01.252.52~10000.99902
CVD81.96.392.55.082.83.66.2512.510~50000.99919
CPA81.27.977.35.177.65.01.252.52~10000.99903
DAS87.83.781.82.878.32.70.250.50.4~2000.99904
DiLy67.03.664.02.361.62.50.250.50.4~2000.99966
DOM84.83.671.64.878.27.46.2512.510~50000.99960
DON77.74.576.72.572.13.26.2512.510~50000.99983
ENN A74.13.979.71.477.02.70.250.50.4~2000.99978
ENN A176.03.382.73.382.23.30.250.50.4~2000.99990
ENN B82.03.784.23.882.73.80.250.50.4~2000.99996
ENN B180.14.384.24.283.72.90.250.50.4~2000.99981
Equi75.04.272.22.977.42.31.252.52~10000.99908
EGCN88.93.985.11.578.94.20.250.50.4~2000.99940
EGCNN78.75.580.74.076.43.80.250.50.4~2000.99916
EGST86.14.384.11.882.92.50.250.50.4~2000.99990
EGSTN80.94.481.03.076.03.00.250.50.4~2000.99936
EGPT86.83.881.74.778.83.00.250.50.4~2000.99919
EGPTN85.83.981.12.775.83.20.250.50.4~2000.99979
EGSN87.613.688.911.678.04.50.250.50.4~2000.99933
FB178.07.370.75.177.02.61.252.52~10000.99999
FB279.17.376.12.873.15.51.252.52~10000.99983
FB363.68.473.22.972.02.21.252.52~10000.99997
Fum71.26.281.83.876.96.76.2512.510~50000.99963
FuX89.34.481.13.576.22.66.2512.510~50000.99948
Glio80.45.481.74.179.74.31.252.52~10000.99983
Grise82.75.483.13.679.32.50.250.50.4~2000.99960
HT-278.97.581.57.380.43.46.2512.510~50000.99936
Lyser68.42.269.73.566.92.40.250.50.4~2000.99923
Melea82.04.380.03.978.23.20.250.50.4~2000.99926
Mono75.67.182.12.879.53.21.252.52~10000.99979
MPA79.110.587.74.484.72.10.250.50.4~2000.99907
NEO91.42.981.34.375.53.01.252.52~10000.99972
O-m-Ster81.04.483.32.581.12.81.252.52~10000.99904
Ostre A90.05.089.04.181.36.96.2512.510~50000.99941
OTA73.67.083.410.381.06.40.250.50.4~2000.99926
OTB70.47.085.83.188.53.60.250.50.4~800.99953
OTC77.25.679.63.378.71.90.250.50.4~2000.99980
Oxa85.33.679.93.476.64.30.250.50.4~2000.99956
Pse A89.54.082.54.379.34.31.252.52~10000.99977
Puro71.13.562.93.267.14.80.250.50.4~2000.99963
Rq C87.02.778.53.074.53.20.250.50.4~2000.99931
Secal A73.55.379.33.475.41.01.252.52~10000.99928
Ster77.810.682.34.080.52.90.250.50.4~2000.99910
T2-triol91.22.782.14.077.93.26.2512.510~50000.99984
T-282.13.383.72.382.34.01.252.52~10000.99971
Ten81.73.383.02.982.53.16.2512.510~50000.99926
Wor-man90.93.483.23.779.76.76.2512.510~50000.99907
α-ZAL86.09.892.65.675.95.11.252.52~10000.99934
α-ZEL106.511.185.24.584.35.61.252.52~10000.99921
β-ZAL92.49.3105.33.989.65.81.252.52~10000.99906
β-ZEL80.18.397.44.486.66.71.252.52~10000.99906
PAT88.73.575.97.673.09.96.2512.510~50000.99995
ZAN88.77.592.34.080.63.51.252.52~10000.99900
ZEN91.711.581.53.678.43.51.252.52~10000.99961
AME96.210.777.33.676.03.86.2512.510~10000.99912
AOH84.49.590.27.083.14.56.2512.510~10000.99868
Table A4. The muti-mycotoxin contamination situation of LS.
Table A4. The muti-mycotoxin contamination situation of LS.
SampleCommentContamination Level (μg/kg)
AFB1AFB2AFG1AFG2AFM1AFM2CITCPAMPA
LS1original shape3.021.4NDND1.0NDNDNDND
LS2original shapeNDNDNDNDNDNDNDNDND
LS3original shapeNDNDNDND1.8NDNDNDND
LS4original shape4.61.3NDNDNDNDNDNDND
LS5original shapeNDNDNDNDNDNDNDND1.3
LS6original shapeNDNDNDNDNDNDNDNDND
LS7original shape1.3NDNDNDNDNDNDNDND
LS8original shapeNDNDNDNDNDNDNDNDND
LS9original shape18.41.3NDNDNDNDNDNDND
LS10original shapeNDNDNDNDNDNDNDNDND
LS11original shapeNDNDNDNDNDNDNDNDND
LS12original shapeNDNDNDNDNDNDNDNDND
LS13original shapeNDNDNDNDNDNDNDNDND
LS14original shape31.61.3NDNDNDNDNDNDND
LS15original shape21.5NDNDNDNDNDNDND27.8
LS16original shape16.41.3NDNDNDNDNDND75.0
LS17original shapeNDNDNDNDNDNDNDNDND
LS18original shapeNDNDNDNDNDNDNDNDND
LS19original shapeNDNDNDNDNDNDNDNDND
LS20original shapeNDNDNDNDNDNDNDNDND
LS21powderNDNDNDNDNDNDND406.9ND
LS22powder12.6NDNDNDNDNDND1043.78.6
LS23powder14.9NDNDNDNDNDND1563.7ND
LS24powder, fresh3638.6160.8NDND47.5NDND12199.8ND
LS25powder, farm-grown4445.4351.917ND8.8189.619.5376.819520.6ND
LS26powder, farm-grown4700.6396.5NDND155.129.8ND23574.6ND
LS27powder, farm-grownNDNDNDNDNDNDND14.6ND
LS28Powder, moldyNDNDNDNDNDNDND5.0ND
LS29Powder, discoloredNDNDNDNDNDNDND2.6ND
Contamination rate (%)41.427.63.43.417.26.93.431.013.8
SampleCommentContamination Level (μg/kg)
ZENBEAENN A1GlioO-m-SterPse ASterTen
LS1original shapeNDNDNDNDNDNDND1.3
LS2original shapeNDNDNDNDNDND6.5ND
LS3original shapeNDNDNDND11.7NDNDND
LS4original shape1.3NDNDNDNDND1.3ND
LS5original shapeND11.11.3NDNDND1.3ND
LS6original shapeNDND1.3ND3.8NDNDND
LS7original shapeNDNDNDNDNDNDNDND
LS8original shapeNDNDNDNDNDND8.2ND
LS9original shapeND14.01.8NDNDNDND9.3
LS10original shapeNDNDNDNDNDNDNDND
LS11original shapeNDNDNDNDNDNDNDND
LS12original shapeNDNDNDNDNDNDNDND
LS13original shapeNDNDNDNDNDNDNDND
LS14original shapeND10.5NDNDNDNDND16.6
LS15original shapeND14.3NDNDNDNDND15.1
LS16original shape34.135.516.2NDNDNDND21.1
LS17original shapeND11.6ND70.7NDNDND3.7
LS18original shape1.310.630.412.5NDNDNDND
LS19original shape1.327.33.38.6NDNDNDND
LS20original shapeND15.91.5NDNDNDNDND
LS21powderNDNDNDNDNDNDNDND
LS22powderNDNDNDNDNDNDNDND
LS23powderNDNDNDNDND6.4NDND
LS24powder, freshNDNDNDND11.4ND7.0ND
LS25powder, farm-grownNDNDNDND158.8ND5.0ND
LS26powder, farm-grownNDNDNDND118.8NDNDND
LS27powder, farm-grownNDNDNDNDNDNDNDND
LS28Powder, moldyNDNDNDNDNDNDNDND
LS29Powder, discoloredNDNDNDNDNDNDNDND
Contamination rate (%)13.831.024.110.317.23.420.720.7
Table A5. The muti-mycotoxin contamination situation of LR.
Table A5. The muti-mycotoxin contamination situation of LR.
SampleCommentVintage YearContamination Level (μg/kg)
ZENDOMDONBEAENN AENN A1ENN BENN B1FB1
LR1slice/NDNDNDNDNDND1.31.38.3
LR2slice/NDNDNDNDNDND13.81.313
LR3slice/2.2NDNDNDNDNDNDND1.3
LR4slice/NDNDNDNDNDNDNDNDND
LR5slice/NDNDNDNDNDNDNDNDND
LR6slice/26.915.115.11.3ND32.377.2104.8ND
LR7slice/NDNDNDNDNDNDNDNDND
LR8slice/NDNDNDNDNDNDNDNDND
LR9slice/NDNDND1.3ND1.31.3ND4.8
LR10slice/NDNDNDNDNDNDNDND1.3
LR11slice/NDNDNDNDNDNDNDNDND
LR12slice/NDNDND2.5ND1.3NDND14.6
LR13slice/NDNDNDNDNDNDNDNDND
LR14slice/NDNDNDNDNDNDNDNDND
LR15slice/NDNDNDNDNDNDNDNDND
LR16slice/NDNDNDNDNDNDNDNDND
LR17slice/NDNDND1.3ND17.761.285.434.9
LR18slice/NDNDNDNDNDNDNDNDND
LR19slice/NDNDNDNDNDNDNDNDND
LR20slice/1.3NDNDNDNDNDNDND1.3
LR21slice/94.6NDND21.4ND27.223.294.810.2
LR22slice/53.1NDNDNDNDNDNDND1.3
LR23slice/NDNDNDNDNDNDNDND1.3
LR24slice/NDNDNDNDNDNDNDND20.7
LR25slice/NDNDNDNDNDNDNDNDND
LR26slice/NDNDNDNDNDNDNDNDND
LR27slice/NDNDNDNDNDNDNDNDND
LR28slice/NDNDNDNDNDNDNDNDND
LR29slice/NDNDNDNDNDNDNDND1.3
LR30slice/NDNDND1.3NDNDNDND8
LR31original shape, a *2018NDNDNDNDNDNDNDNDND
LR32original shape, b201839.5NDNDNDNDNDNDNDND
LR33original shape, c2018NDNDNDNDNDNDNDNDND
LR34original shape, d2018NDNDNDNDNDNDNDNDND
LR35original shape, d201842NDNDNDNDNDNDNDND
LR36original shape, d2018NDNDNDNDNDNDNDNDND
LR37original shape, d2018NDNDNDNDNDNDNDNDND
LR38original shape2019NDNDNDNDNDNDNDNDND
LR39original shape, d2019NDNDNDNDNDNDNDNDND
LR40original shape, d2019NDNDNDNDNDNDNDNDND
LR41original shape, d2019NDNDNDNDNDNDNDNDND
LR42original shape, d2019NDNDNDNDNDNDNDNDND
LR43original shape, d2019NDNDNDNDNDNDNDNDND
LR44original shape, d2019NDNDNDNDNDNDNDNDND
LR45original shape, d2019NDNDNDNDNDNDNDNDND
LR46slice, d2019NDNDNDNDNDNDNDNDND
LR47slice, d2019NDNDNDNDNDNDNDNDND
LR48slice, d2019NDNDNDNDNDNDNDNDND
LR49slice, d201940.4NDNDNDNDNDNDNDND
LR50original shape, d2016NDNDNDNDNDNDNDNDND
LR51original shape, d2016NDNDNDNDNDNDNDNDND
LR52original shape, d2018NDNDNDNDNDND1.6NDND
LR53original shape, d2018NDNDNDNDNDNDNDNDND
LR54original shape, d2015NDNDNDNDNDND1.1NDND
LR55original shape, d2015NDNDND1.1NDNDNDNDND
LR56original shape, d2015NDNDND2NDNDNDNDND
LR57original shape, d2015NDNDND1NDNDNDNDND
LR58original shape, d2017NDNDNDNDNDND1.7NDND
LR59original shape, d2017NDNDNDNDNDNDNDNDND
LR60original shape, c201934.1NDND1.8NDND1.3NDND
LR61original shape, c2019NDNDND5.9NDND1.4NDND
LR62original shape, c20155.3NDND90.215.938.7119.790.6ND
LR63original shape, e2018NDNDND7.25.21453.231.0ND
LR64original shape, e2019NDNDND483.22.318.2166.884.5ND
LR65original shape, e2020NDNDNDND5.931.2188.2100.8ND
LR66original shape, a2018NDNDND21.8NDND3.21.9ND
LR67original shape, a2019NDNDNDNDNDND3.51.9ND
LR68original shape, a20205.8NDNDNDNDND1.1NDND
LR69original shape, c2019NDNDND3.2NDNDNDNDND
LR70original shape, c2020NDNDND3.2NDND3.11.8ND
LR71original shape, c2019NDNDNDNDNDND1.4NDND
LR72original shape, c2019NDNDNDNDNDNDNDNDND
LR73original shape, c2020NDNDNDNDNDND1.71.1ND
LR74original shape, b201717.4NDND4.9NDND1NDND
LR75original shape, b2020NDNDND10.8NDND2.11.5ND
LR76original shape, b2018NDNDNDNDNDNDNDNDND
LR77original shape, b2019NDNDND2.1NDND1.9NDND
Contamination rate (%)15.61.31.326.05.211.731.218.218.2
SampleCommentVintage yearContamination level (μg/kg)
FB2MeleaOTAMPAPse ACITAOHAMEDiLy
LR1slice/NDND3.2ND15.34.6NDNDND
LR2slice/NDNDNDNDND1.3NDNDND
LR3slice/NDNDND1.3NDNDND1.3ND
LR4slice/NDNDNDNDNDNDNDNDND
LR5slice/NDNDNDNDNDNDNDNDND
LR6slice/NDNDND1.3NDNDNDNDND
LR7slice/NDNDNDNDNDNDNDNDND
LR8slice/NDNDNDNDNDNDND14.5ND
LR9slice/14.4NDNDNDNDNDNDNDND
LR10slice/NDND2.4ND4.6NDNDNDND
LR11slice/NDNDNDNDNDNDNDNDND
LR12slice/6.3NDNDNDNDNDNDNDND
LR13slice/NDNDNDNDNDNDNDNDND
LR14slice/NDNDNDNDNDNDND19.2ND
LR15slice/NDNDNDNDNDNDNDNDND
LR16slice/NDNDND107.0NDNDNDNDND
LR17slice/10.2NDND99.8NDNDNDNDND
LR18slice/NDNDNDNDNDNDNDNDND
LR19slice/NDNDNDNDNDNDNDNDND
LR20slice/12.4NDNDNDNDNDND1.3ND
LR21slice/NDNDND1.3NDNDNDNDND
LR22slice/NDND6.4NDNDNDNDNDND
LR23slice/1.3NDNDNDNDNDNDNDND
LR24slice/8.95.7ND1.3NDNDNDNDND
LR25slice/NDNDNDNDNDNDNDNDND
LR26slice/NDNDNDNDNDNDNDNDND
LR27slice/NDNDNDNDNDNDNDNDND
LR28slice/1.3NDNDNDNDNDNDNDND
LR29slice/ND24.8NDNDNDNDND15.9ND
LR30slice/1.31.3NDNDNDNDNDNDND
LR31original shape, a *2018NDNDNDNDNDNDNDNDND
LR32original shape, b2018NDNDNDNDNDNDNDNDND
LR33original shape, c2018NDNDNDNDNDNDNDNDND
LR34original shape, d2018NDNDNDNDNDNDNDNDND
LR35original shape, d2018NDNDNDNDNDNDNDNDND
LR36original shape, d2018NDNDNDNDNDNDNDNDND
LR37original shape, d2018NDNDNDNDNDNDNDNDND
LR38original shape2019NDNDNDNDNDNDNDNDND
LR39original shape, d2019NDNDNDNDNDNDNDNDND
LR40original shape, d2019NDNDNDNDNDNDNDNDND
LR41original shape, d2019NDNDNDNDNDNDNDNDND
LR42original shape, d2019NDNDNDNDNDNDNDNDND
LR43original shape, d2019NDNDNDNDNDNDNDNDND
LR44original shape, d2019NDNDNDNDNDNDNDNDND
LR45original shape, d2019NDNDNDNDNDNDNDNDND
LR46slice, d2019NDNDNDNDNDNDNDNDND
LR47slice, d2019NDNDNDNDNDNDNDNDND
LR48slice, d2019NDNDNDNDNDNDNDNDND
LR49slice, d2019NDNDNDNDNDNDNDNDND
LR50original shape, d2016NDNDNDNDNDNDNDNDND
LR51original shape, d2016NDNDNDNDNDNDNDNDND
LR52original shape, d2018NDNDNDNDNDNDNDNDND
LR53original shape, d2018NDNDNDNDNDNDNDNDND
LR54original shape, d2015NDNDNDNDNDNDNDND2.5
LR55original shape, d2015NDNDNDNDNDNDNDNDND
LR56original shape, d2015NDNDNDNDNDNDNDNDND
LR57original shape, d2015NDNDNDNDNDNDNDNDND
LR58original shape, d2017NDNDNDNDNDNDNDNDND
LR59original shape, d2017NDNDNDNDNDNDNDNDND
LR60original shape, c2019NDNDNDNDNDNDNDNDND
LR61original shape, c2019NDNDNDNDNDNDNDNDND
LR62original shape, c2015NDNDNDNDNDNDNDNDND
LR63original shape, e2018ND5.1NDNDNDNDNDNDND
LR64original shape, e2019NDNDNDNDNDND85.5NDND
LR65original shape, e2020NDNDNDNDNDNDNDNDND
LR66original shape, a2018NDNDNDNDNDNDNDNDND
LR67original shape, a2019NDNDNDNDNDNDNDNDND
LR68original shape, a2020NDNDNDNDNDNDNDNDND
LR69original shape, c2019NDNDNDNDNDNDNDNDND
LR70original shape, c2020NDNDNDNDNDNDNDNDND
LR71original shape, c2019NDNDNDNDNDNDNDNDND
LR72original shape, c2019NDNDNDNDNDNDNDNDND
LR73original shape, c2020NDNDNDNDNDNDNDNDND
LR74original shape, b2017NDNDNDNDNDNDNDNDND
LR75original shape, b2020NDNDNDNDNDNDNDNDND
LR76original shape, b2018ND1.1NDNDNDNDNDNDND
LR77original shape, b2019NDNDNDNDNDNDNDNDND
Contamination rate (%)10.46.53.97.82.62.61.36.51.3
*: a: Jilin, b: Ningxia, c: Neimenggu, d: Xinjiang, e: Gansu.
Table A6. The muti-mycotoxin contamination situation of CP.
Table A6. The muti-mycotoxin contamination situation of CP.
SampleCommentContamination Level (μg/kg)
BEAENN A1ENN BENN B1MPA
CP1original shapeNDNDNDND6.7
CP2original shapeNDNDNDNDND
CP3original shapeNDNDNDND9.8
CP4original shapeNDNDNDND14
CP5original shapeNDNDNDNDND
CP6original shapeNDNDNDNDND
CP7original shapeNDNDNDND25.7
CP8original shapeNDNDNDNDND
CP9original shapeNDNDNDND24.1
CP10original shapeNDNDNDNDND
CP11original shapeNDNDNDNDND
CP12original shapeNDNDNDNDND
CP13original shapeNDNDNDND15.2
CP14original shapeNDNDNDND16
CP15original shape6.9319.36.1ND
CP16original shapeNDNDNDND17.9
CP17original shapeNDNDNDNDND
CP18original shapeNDNDNDND14.2
CP19original shapeNDNDNDNDND
CP20original shape142.715.84.711.4
CP21original shapeNDNDNDNDND
CP22original shapeNDNDNDND15.8
CP23original shapeNDNDNDNDND
CP24original shapeNDNDNDNDND
CP25original shapeNDNDNDNDND
CP26original shapeNDNDNDND20.5
CP27original shapeNDNDNDND18.3
CP28original shapeNDNDNDNDND
CP29original shapeNDNDNDND16
CP30original shapeNDNDNDNDND
CP31original shapeNDNDNDND22.3
CP32original shapeNDNDNDNDND
CP33original shapeNDNDNDND12
CP34original shape8.72.730.513.222.8
CP35original shapeNDNDNDNDND
CP36original shapeNDNDNDNDND
CP37original shapeNDNDNDND16.8
CP38original shapeNDNDNDND34.4
CP39original shapeNDNDNDNDND
CP40original shapeNDNDNDND14.2
CP41original shapeNDNDNDNDND
CP42original shapeNDNDNDNDND
CP43original shapeNDNDNDND21
CP44original shapeNDNDNDNDND
CP45original shapeNDNDNDND15.8
CP46original shapeNDNDNDNDND
CP47original shapeNDNDNDND15
CP48original shape12.11.216.43.7ND
CP49original shape10.51.622.75.122.0
CP50original shapeNDNDNDNDND
CP51original shapeNDNDNDND16.3
CP52original shapeNDNDNDNDND
CP53original shapeNDNDNDND10.5
CP54original shapeNDNDNDNDND
CP55original shapeNDNDNDND11.9
CP56original shapeNDNDNDND21.4
CP57original shapeNDNDNDND16.5
CP58original shapeNDNDNDNDND
CP59original shapeNDNDNDNDND
CP60original shapeNDNDNDNDND
CP61original shapeNDNDNDNDND
CP62original shapeNDNDNDND18.3
CP63original shapeNDNDNDNDND
CP64original shape5.57.365.820.016.8
CP65original shapeNDNDNDNDND
CP66original shapeNDNDNDND22.8
CP67original shapeNDNDNDNDND
CP68original shapeNDNDNDND12.1
CP69original shapeNDNDNDND22.4
CP70original shapeNDNDNDNDND
CP71original shapeNDNDNDNDND
CP72original shapeNDNDNDND16.7
CP73original shapeNDNDNDNDND
CP74original shapeNDNDNDND16.8
CP75original shapeNDNDNDNDND
CP76original shapeNDNDNDNDND
CP77original shapeNDNDNDND20.4
CP78original shapeNDNDNDNDND
CP79original shapeNDNDNDND15.6
CP80original shapeNDNDNDNDND
CP81original shapeNDNDNDND19.9
CP82original shapeNDNDNDNDND
CP83original shapeNDNDNDNDND
CP84original shapeNDNDNDND26.7
CP85original shapeNDNDNDNDND
CP86original shapeNDNDNDNDND
CP87original shapeNDNDNDNDND
CP88original shapeNDNDNDNDND
CP89original shapeNDNDNDND32.3
CP90original shapeNDNDNDNDND
CP91original shapeNDNDNDNDND
CP92original shapeNDNDNDND11.8
CP93original shape6.215.4167.234.612.4
CP94original shape5.17.381.320.214.5
CP95original shape8.83.735.91121.8
CP96original shapeNDNDNDNDND
CP97original shapeNDNDNDNDND
CP98original shapeNDNDNDNDND
CP99original shapeNDNDNDND33
CP100original shape11.511.7100.425.621.7
CP101original shapeNDNDNDNDND
CP102original shapeNDNDNDNDND
CP103original shapeNDNDNDNDND
CP104original shapeNDNDNDNDND
CP105original shapeNDNDNDNDND
CP106original shapeNDNDNDNDND
CP107original shapeNDNDNDNDND
CP108original shapeNDNDNDNDND
CP109original shapeNDNDNDNDND
CP110original shapeNDNDNDNDND
CP111original shapeNDNDNDNDND
CP112original shape10.3ND10.73.3ND
CP113powderNDNDNDNDND
CP114powderNDNDNDNDND
CP115powderNDNDNDNDND
CP116original shapeNDNDNDNDND
CP117original shapeNDNDNDNDND
CP118original shapeNDNDNDNDND
CP119original shapeNDNDNDNDND
CP120original shapeNDNDNDNDND
CP121original shapeNDNDNDNDND
CP122original shapeNDNDNDNDND
CP123original shapeNDNDNDNDND
CP124original shapeNDNDNDNDND
CP125original shapeNDNDNDNDND
CP126original shapeNDNDNDNDND
CP127original shapeNDNDNDNDND
CP128original shapeNDNDNDNDND
CP129original shapeNDNDNDNDND
CP130original shapeNDNDNDNDND
CP131original shapeNDNDNDNDND
Contamination rate (%)8.47.68.48.435.9
Table A7. The muti-mycotoxin contamination situation of CS.
Table A7. The muti-mycotoxin contamination situation of CS.
SampleCommentContamination Level (μg/kg)
15-Asp3-ADONApiciBEADONDASEquiFB1FB2
CS1original shape21.3NDNDNDND37.1ND24.310.5
CS2original shape2.2NDNDNDND2.3ND13.23.0
CS3original shape6.8NDND0.2100.47.3ND353.050.5
CS4original shape1.0NDNDNDND1.9ND91.59.0
CS5original shape1.0NDNDND41.72.6ND98.05.7
CS6original shape1.620.6NDND32.32.8NDNDND
CS7original shape1.0NDND2.01.02.71.0NDND
CS8original shape1.0NDNDND1.01.5ND5.0ND
CS9original shape1.0NDNDND1.00.7ND96.35.5
CS10original shape5.2ND0.6ND12.33.8ND36.4ND
CS11original shape4.6NDNDND422.33.0NDNDND
CS12original shape2.1NDNDNDND9.4NDNDND
CS13original shapeNDNDNDND21.51.2ND18.32.3
CS14original shape8.8NDNDNDND4.4ND4.1ND
CS15original shape10.5NDNDNDND9.6ND56.410.7
CS16original shapeNDNDNDNDNDNDND5.6ND
CS17original shapeNDNDND3.1NDNDNDND7.1
CS18original shapeNDNDND11.1NDNDND18.119.6
CS19original shapeNDNDNDNDNDNDNDND3.9
CS20original shapeNDNDNDNDNDNDND11.18.4
CS21original shapeNDNDND5.1NDNDND3.21.1
CS22original shapeNDNDND46.2NDNDND2.9ND
CS23original shapeNDNDND4.5NDNDND23.52.4
CS24original shapeNDNDND18.6NDNDND10.11.1
CS25original shapeNDNDND13.7NDNDND54.623.4
CS26original shapeNDNDND13.9NDNDND26.623.0
CS27original shapeNDNDNDNDNDNDND3.9ND
CS28original shapeNDNDND14.2NDNDND172.838.1
CS29original shapeNDNDND50.0NDNDND20.43.0
CS30original shapeNDNDND5.8NDNDND5.27.4
CS31original shapeNDNDNDNDNDNDNDNDND
CS32original shapeNDNDNDNDNDNDNDNDND
CS33original shapeNDNDNDNDNDNDNDNDND
CS34original shapeNDNDND11.7NDNDND21.910.3
CS35original shapeNDNDNDNDNDNDNDNDND
CS36original shapeNDNDND2.1NDNDND73.413.9
CS37original shapeNDNDNDNDNDNDNDNDND
CS38original shapeNDNDNDNDNDNDNDNDND
CS39original shapeNDNDNDNDNDNDND4.1ND
CS40original shapeNDNDNDNDNDNDND16.25.5
CS41original shapeNDNDND4.4NDNDND43.012.6
CS42original shapeNDNDND109.9NDNDND69.817.5
CS43original shapeNDNDND56.2NDNDND55.753.9
CS44original shapeNDNDNDNDNDNDNDNDND
CS45original shapeNDNDNDNDNDNDND12.42.2
CS46original shapeNDNDND111.1NDNDND54.413.2
CS47original shapeNDNDNDNDNDNDNDNDND
Contamination rate (%)29.82.12.140.419.131.92.170.259.6
SamplecommentContamination level (μg/kg)
FB3FuXMPAZENZANα-ZELβ-ZALβ-ZELCVD
CS1original shape2.4NDNDNDNDNDNDNDND
CS2original shape6.4NDNDNDNDNDNDNDND
CS3original shape27.250.8ND59.123.021.722.522.5ND
CS4original shapeNDNDND53.437.235.935.835.6ND
CS5original shape8.3ND24.562.027.151.271.827.0ND
CS6original shapeND1.0ND10.0NDNDNDNDND
CS7original shapeNDNDNDNDNDNDNDNDND
CS8original shapeNDNDND8.5NDNDNDNDND
CS9original shapeND36.7ND9.6NDNDNDNDND
CS10original shapeND21.0ND11.2NDNDNDNDND
CS11original shapeND52.2ND9.4NDNDNDNDND
CS12original shapeND29.3ND96.7NDNDNDNDND
CS13original shapeND13.6ND9.8NDNDNDNDND
CS14original shapeNDNDND10.8NDNDNDNDND
CS15original shape10.0NDND29.1NDNDNDND25.7
CS16original shapeNDNDND12.9NDNDNDNDND
CS17original shapeNDNDNDNDNDNDNDNDND
CS18original shapeNDNDNDNDNDNDNDNDND
CS19original shapeNDNDND69.13.8NDNDNDND
CS20original shapeNDNDND96.82.3NDNDNDND
CS21original shapeNDNDNDNDNDNDNDNDND
CS22original shapeNDNDNDNDNDNDNDNDND
CS23original shapeNDNDND5.6NDNDNDNDND
CS24original shapeNDNDNDNDNDNDNDNDND
CS25original shapeNDNDNDNDNDNDNDNDND
CS26original shapeNDNDNDNDNDNDNDNDND
CS27original shapeNDNDND14.5NDNDNDNDND
CS28original shapeNDNDND77.52.0NDNDNDND
CS29original shapeNDNDNDNDNDNDNDNDND
CS30original shapeNDNDND6.6NDNDNDNDND
CS31original shapeNDNDND105.11.2NDNDNDND
CS32original shapeNDNDND28.81.0NDNDNDND
CS33original shapeNDNDNDNDNDNDNDNDND
CS34original shapeNDNDND81.43.8NDNDNDND
CS35original shapeNDNDND65.42.1NDNDNDND
CS36original shapeNDNDNDNDNDNDNDNDND
CS37original shapeNDNDNDNDNDNDNDNDND
CS38original shapeNDNDNDNDNDNDNDNDND
CS39original shapeNDNDND13.7NDNDNDNDND
CS40original shapeNDNDND49.5NDNDNDNDND
CS41original shapeNDNDND206.96.0NDNDNDND
CS42original shapeNDNDND4.1NDNDNDNDND
CS43original shapeNDNDNDNDNDNDNDNDND
CS44original shapeNDNDNDNDNDNDNDNDND
CS45original shapeNDNDND13.2NDNDNDNDND
CS46original shapeNDNDNDNDNDNDNDNDND
CS47original shapeNDNDNDNDNDNDNDNDND
Contamination rate (%)10.614.92.159.623.46.46.46.42.1
SamplecommentContamination level (μg/kg)
GriseCPA7-D-GAFB1AFB2OTASterGlioTen
CS1original shapeND13.3ND0.2NDNDNDND1.7
CS2original shapeND3.1NDNDNDNDNDNDND
CS3original shapeNDNDND0.6ND3.2NDND0.5
CS4original shapeNDNDND0.4NDNDNDND0.2
CS5original shapeNDNDND5.12.0ND0.8ND0.2
CS6original shapeNDNDNDNDNDNDNDNDND
CS7original shapeNDNDNDNDNDNDNDNDND
CS8original shapeNDNDNDNDNDNDNDNDND
CS9original shapeNDNDND0.4NDNDND1.0ND
CS10original shapeNDND54.4NDNDNDND57.8ND
CS11original shape1.8NDND4.90.3NDNDNDND
CS12original shapeNDNDNDNDNDNDNDNDND
CS13original shapeNDNDNDNDNDNDNDNDND
CS14original shapeNDNDND0.1NDNDNDNDND
CS15original shapeNDNDNDNDNDNDNDNDND
CS16original shapeNDNDND0.3NDNDNDNDND
CS17original shapeNDNDNDNDNDNDNDNDND
CS18original shapeNDNDNDNDNDNDNDNDND
CS19original shapeNDNDNDNDNDNDNDNDND
CS20original shapeNDNDND1.0NDNDNDNDND
CS21original shapeNDNDNDNDNDNDNDNDND
CS22original shapeNDNDNDNDNDNDNDNDND
CS23original shapeNDNDNDNDNDNDNDNDND
CS24original shapeNDNDNDNDNDNDNDNDND
CS25original shapeNDNDNDNDNDNDNDNDND
CS26original shapeNDNDNDNDNDNDNDNDND
CS27original shapeNDNDNDNDNDNDNDNDND
CS28original shapeNDNDNDNDNDNDNDNDND
CS29original shapeNDNDNDNDNDNDNDNDND
CS30original shapeNDNDNDNDNDNDNDNDND
CS31original shapeNDNDNDNDNDNDNDNDND
CS32original shapeNDNDNDNDNDNDNDNDND
CS33original shapeNDNDNDNDNDNDNDNDND
CS34original shapeNDNDNDNDNDNDNDNDND
CS35original shapeNDNDNDNDNDNDNDNDND
CS36original shapeNDNDNDNDNDNDNDNDND
CS37original shapeNDNDNDNDNDNDNDNDND
CS38original shapeNDNDNDNDNDNDNDNDND
CS39original shapeNDNDNDNDNDNDNDNDND
CS40original shapeNDNDND3.31.2NDNDNDND
CS41original shapeNDNDNDNDNDNDNDNDND
CS42original shapeNDNDND2.4NDNDNDNDND
CS43original shapeNDNDND1.1NDNDNDNDND
CS44original shapeNDNDNDNDNDNDNDNDND
CS45original shapeNDNDNDND0.7NDNDNDND
CS46original shapeNDNDNDNDNDNDNDNDND
CS47original shapeNDNDNDNDNDNDNDNDND
Contamination rate (%)2.14.32.125.58.52.12.14.38.5
Table A8. Source and purity of 73 reference standards.
Table A8. Source and purity of 73 reference standards.
No.MycotoxinLot.PurityCompany
115-AcetoxyscirpenolAS46621699%Apollo Scientific, Stockport, UK
215-AcetyldeoxynivalenolL14261A99.9%Romer Labs, Tullin, Austria
33-AcetyldeoxynivalenolSZBD036XV98.3%Sigma-Aldrich, Laramie, USA
47-Dechloro Griseofulvin10-KPA-158-498.85%Toronto Research Chemicals, Toronto, Canada
5Aflatoxin B1LKB0P7598%J&K Scientific, Shanghai, China
6Aflatoxin B2L260Q6398%J&K Scientific, Shanghai, China
7Aflatoxin G128209598%J&K Scientific, Shanghai, China
8Aflatoxin G2LQ10Q7698.72%J&K Scientific, Shanghai, China
9Aflatoxin M12-AJK-95-195%Toronto Research Chemicals, Toronto, Canada
10Aflatoxin M2LB40Q5695%J&K Scientific, Shanghai, China
11Aflatoxin P12-BSR-92-398%Toronto Research Chemicals, Toronto, Canada
12AgroclavineL13212A98.2%Chiron AS, Trondheim, Norway
13AnisomycinLDC0Q8599.04%J&K Scientific, Shanghai, China
14Apicidin3-PQY-12-196%Toronto Research Chemicals, Toronto, Canada
15BeauvericinLHB0P0799%J&K Scientific, Shanghai, China
16Chaetocin124M4012V98%Sigma-Aldrich, Laramie, USA
17Chetomin36782097.17%International Laboratory USA, San Bruno, USA
18CitrininLH40O3398.5J&K Scientific, Shanghai, China
19CitreoviridinL280Q4797.17%J&K Scientific, Shanghai, China
20Cyclopiazonic acid085M4081V98%Sigma-Aldrich, Laramie, USA
21DiacetoxyscirpenolL280Q3599%J&K Scientific, Shanghai, China
22Dihydrolysergamide3-NAV-62-198%Toronto Research Chemicals, Toronto, Canada
23Deepoxy-deoxynivalenolL16103A98%Chiron AS, Trondheim, Norway
24DeoxynivalenolLR10Q10399.83%J&K Scientific, Shanghai, China
25Enniatin AAL26.12999%BioAustralis, New South Wales, Australia
26Enniatin A1AL26.2199%BioAustralis, New South Wales, Australia
27Enniatin BAL26.13599%BioAustralis, New South Wales, Australia
28Enniatin B1AL23.11599%BioAustralis, New South Wales, Australia
29Equisetin2-LWJ-95-193.06%Toronto Research Chemicals, Toronto, Canada
30ErgocornineL14331F97.8%Chiron AS, Trondheim, Norway
31ErgocorninineL15212A97.8%Chiron AS, Trondheim, Norway
32ErgocristineLK70P8399%Chiron AS, Trondheim, Norway
33ErgocristinineL15071E99%Chiron AS, Trondheim, Norway
34ErgocryptineL14331E97.6%Chiron AS, Trondheim, Norway
35ErgocryptinineL15071D97.6%Chiron AS, Trondheim, Norway
36ErgosineL15282E99.9%Chiron AS, Trondheim, Norway
37Fumonisin B1SZBF083XV98.5%Sigma-Aldrich, Laramie, USA
38Fumonisin B2SZBF089XV98.5%Sigma-Aldrich, Laramie, USA
39Fumonisin B3SZBE295XV98.5%Sigma-Aldrich, Laramie, USA
40FumagillinLK70P6798%J&K Scientific, Shanghai, China
41Fusarenon XL14141F99.9%Romer Labs, Tullin, Austria
42GliotoxinLJ50Q7799%J&K Scientific, Shanghai, China
43GriseofulvinL5C0O2799.6%J&K Scientific, Shanghai, China
44HT-2 toxinSZBA287X99.9%Sigma-Aldrich, Laramie, USA
45Lysergamide1-GAC-130-299.6%Toronto Research Chemicals, Toronto, Canada
46Meleagrin2-LXM-37-197%Toronto Research Chemicals, Toronto, Canada
47MonocerinAS46736399.9%Apollo Scientific, Stockport, UK
48Mycophenolic AcidLJ20O3998%J&K Scientific, Shanghai, China
49NeosolaniolSZBD144XV99.3%Sigma-Aldrich, Laramie, USA
50O-methylsterigmatocystin00013645-20199%ChromaDex Standards, Irvine, USA
51Ostreogrycin A36104799%International Laboratory USA, San Bruno, USA
52Ochratoxin A5-JKS-45-398%Toronto Research Chemicals, Toronto, Canada
53Ochratoxin BLAC0P3099%J&K Scientific, Shanghai, China
54Ochratoxin C5-UKS-34-198%Toronto Research Chemicals, Toronto, Canada
55OxalineAS46371492.87%Apollo Scientific, Stockport, UK
56Pseurotin A3-PQY-14-195%Toronto Research Chemicals, Toronto, Canada
57Puromycin00016435-93999%ChromaDex Standards, Irvine, USA
58Roquefortine CAS46798598%Apollo Scientific, Stockport, UK
59Secalonic acid DL15471S99%Chiron AS, Trondheim, Norway
60SterigmatocystinSZBF020XV99.3%Sigma-Aldrich, Laramie, USA
61T-2-triolL15354A98.2%Chiron AS, Trondheim, Norway
62T-2 toxin2J0A0299%Pribolab, Qingdao, China
63Tentoxin1I1J2899%Pribolab, Qingdao, China
64WortmanninL6B0P6898%J&K Scientific, Shanghai, China
65α-zearalanol086K402497%Sigma-Aldrich, Laramie, USA
66α-zearalenol056W4021V97%Sigma-Aldrich, Laramie, USA
67β-zearalanol115K403798%Sigma-Aldrich, Laramie, USA
68β-zearalenol115M4019V98%Sigma-Aldrich, Laramie, USA
69PatulinLAS46798699%Apollo Scientific, Stockport, UK
70Zearalanone4-RNP-61-196%Toronto Research Chemicals, Toronto, Canada
71ZearalenoneSZBC355XV99.3%Sigma-Aldrich, Laramie, USA
72Alternariol-methylether045M4017V96%Sigma-Aldrich, Laramie, USA
73Alternariol084M4167V96%Sigma-Aldrich, Laramie, USA

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Toxins 17 00052 g001
Figure 1. The optimal ion pairs in different plants for certain mycotoxin.
Figure 1. The optimal ion pairs in different plants for certain mycotoxin.
Toxins 17 00052 g001
Toxins 17 00052 g002
Figure 2. Comparation of chromatograms for the salt-out procedure. (a) Chromatography of quantitative (Q) and qualitative (q) ion pairs for sample without salt-out procedure; (b) chromatography of quantitative (Q) and qualitative (q) ion pairs for sample with salt-out procedure.
Figure 2. Comparation of chromatograms for the salt-out procedure. (a) Chromatography of quantitative (Q) and qualitative (q) ion pairs for sample without salt-out procedure; (b) chromatography of quantitative (Q) and qualitative (q) ion pairs for sample with salt-out procedure.
Toxins 17 00052 g002
Toxins 17 00052 g003
Figure 3. Recoveries of representative mycotoxins in different salt package.
Figure 3. Recoveries of representative mycotoxins in different salt package.
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Toxins 17 00052 g004
Figure 4. Distribution of susceptible fungi types of four plants.
Figure 4. Distribution of susceptible fungi types of four plants.
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Toxins 17 00052 g005
Figure 5. Content percentage of co-occurrence number of mycotoxins in four matrices.
Figure 5. Content percentage of co-occurrence number of mycotoxins in four matrices.
Toxins 17 00052 g005
Toxins 17 00052 g006
Figure 6. Comparison of the concentration of the regulated mycotoxins in lotus seed and coix seed.
Figure 6. Comparison of the concentration of the regulated mycotoxins in lotus seed and coix seed.
Toxins 17 00052 g006
Toxins 17 00052 g007
Figure 7. Comparison of mycotoxin levels (mean with 95% CI) between intact and processed forms of LS (A) and LR (B).
Figure 7. Comparison of mycotoxin levels (mean with 95% CI) between intact and processed forms of LS (A) and LR (B).
Toxins 17 00052 g007
Toxins 17 00052 g008
Figure 8. Comparison of mycotoxin detection rates between intact and processed forms of LS (A) and LR (B).
Figure 8. Comparison of mycotoxin detection rates between intact and processed forms of LS (A) and LR (B).
Toxins 17 00052 g008
Toxins 17 00052 g009
Figure 9. MOE values from lotus seed and coix seed consumption for Chinese people.
Figure 9. MOE values from lotus seed and coix seed consumption for Chinese people.
Toxins 17 00052 g009
Toxins 17 00052 g010
Figure 10. HQ values from lotus seed and coix seed consumption for Chinese people.
Figure 10. HQ values from lotus seed and coix seed consumption for Chinese people.
Toxins 17 00052 g010
Table 1. Chemical Information of 73 mycotoxins.
Table 1. Chemical Information of 73 mycotoxins.
No.MycotoxinAbbreviationFormula
115-Acetoxyscirpenol15-AspC17H24O6
215-Acetyldeoxynivalenol15-ADONC17H22O7
33-Acetyldeoxynivalenol3-ADONC17H22O7
47-Dechloro Griseofulvin7-D-GC17H18O6
5Aflatoxin B1AFB1C17H12O6
6Aflatoxin B2AFB2C17H14O6
7Aflatoxin G1AFG1C17H12O7
8Aflatoxin G2AFG2C17H14O7
9Aflatoxin M1AFM1C17H12O7
10Aflatoxin M2AFM2C17H14O7
11Aflatoxin P1AFP1C16H10O6
12AgroclavineAgroC16H18N2
13AnisomycinAnisC14H19NO4
14ApicidinApiciC34H50N5O6
15BeauvericinBEAC45H57N3O9
16ChaetocinChaeC30H28N6O6S4
17ChetominCheC31H30N6O6S4
18CitrininCITC13H14O5
19CitreoviridinCVDC23H30O6
20Cyclopiazonic acidCPAC20H20N2O3
21DiacetoxyscirpenolDASC19H26O7
22DihydrolysergamideDiLyC16H19N3O
23Deepoxy-deoxynivalenolDOMC15H20O5
24DeoxynivalenolDONC15H20O6
25Enniatin AENN AC36H63N3O9
26Enniatin A1ENN A1C35H61N3O9
27Enniatin BENN BC33H57N3O9
28Enniatin B1ENN B1C34H59N3O9
29EquisetinEquiC22H31NO4
30ErgocornineEGCNC31H39N5O5
31ErgocorninineEGCNNC31H39N5O5
32ErgocristineEGSTC35H39N5O5
33ErgocristinineEGSTNC35H39N5O5
34ErgocryptineEGPTC32H41N5O5
35ErgocryptinineEGPTNC32H41N5O5
36ErgosineEGSNC30H37N5O5
37Fumonisin B1FB1C34H59NO15
38Fumonisin B2FB2C34H59NO14
39Fumonisin B3FB3C34H59NO14
40FumagillinFumC26H34O7
41Fusarenon XFuXC17H22O8
42GliotoxinGlioC13H14N2O4S2
43GriseofulvinGriseC17H17ClO6
44HT-2 toxinHT-2C22H32O8
45LysergamideLyserC16H17N3O
46MeleagrinMeleaC23H23N5O4
47MonocerinMONOC16H20O6
48Mycophenolic AcidMPAC17H20O6
49NeosolaniolNEOC19H26O8
50O-methylsterigmatocystinO-m-SterC19H14O6
51Ostreogrycin AOstre AC28H35N3O7
52Ochratoxin AOTAC20H18ClNO6
53Ochratoxin BOTBC20H19NO6
54Ochratoxin COTCC22H22ClNO6
55OxalineOxaC24H25N5O4
56Pseurotin APse AC22H25NO8
57PuromycinPuroC22H29N7O5
58Roquefortine CRq CC22H23N5O2
59Secalonic acid DSecal Acid DC32H30O14
60SterigmatocystinSterC18H12O6
61T-2-triolT2-triC20H30O7
62T-2 toxinT-2C24H34O9
63TentoxinTenC22H30N4O4
64WortmanninWor-manC23H24O8
65α-zearalanolα-ZALC18H26O5
66α-zearalenolα-ZELC18H24O5
67β-zearalanolβ-ZALC18H26O5
68β-zearalenolβ-ZELC18H24O5
69PatulinPATC7H6O4
70ZearalanoneZANC18H24O5
71ZearalenoneZENC18H22O5
72Alternariol-methyletherAMEC15H12O5
73AlternariolAOHC14H10O5
Table 2. Optimized MS/MS parameters for the analytes studied.
Table 2. Optimized MS/MS parameters for the analytes studied.
MycotoxinMSRT (min)Precursor IonDP (V)Product Ion
12345
IonCE (V)IonCE (V)IonCE (V)IonCE (V)IonCE (V)
15-Asp[M+NH4]+8.6342.010265.313307.212
15-ADON[M+H]+7.6339.1130137.123261.116321.119261.016304.419
3-ADON[M+H]+7.6339.1130231.117203.120304.119181.224
7-D-G[M+H]+11.8319.122181.224251.124
AFB1[M+H]+10.5313.0200241.050285.133269.042269.042214.040
AFB2[M+H]+9.7315.1200287.135259.140243.152203.050271.046
AFG1[M+H]+9.0329.1200243.135215.145311.130283.033
AFG2[M+H]+8.4331.1200313.133245.140217.147257.042189.055
AFM1[M+H]+8.5329.160273.133259.233
AFM2[M+H]+7.9331.1140273.147259.347285.047
AFP1[M+H]+8.9299.0180271.133215.139187.143201.139
Agro[M+H]+7.2239.120183.133168.236198.235207.935
Anis[M+H]+6.2266.220206.122121.035
Apici[M+H]+15.6624.3190464.325592.321
BEA[M+NH4]+16.4801.410244.240262.040
Chae[M+H]+15.0697.190348.026284.336
Che[M+H]+15.3711.2150644.917647.217348.028
CIT[M+H]+12.3251.290233.125205.135
CVD[M+H]+15.1403.2220139.030297.120
CPA[M+H]+15.7337.2140182.025196.047319.135
DAS[M+H]+10.4384.240307.315229.120247.119
DiLy[M+H]+4.1270.140225.130168.130
DOM[M+H]+6.3281.1130215.118233.116
DON[M+H]+4.8297.1120249.117231.118
ENN A[M+NH4]+16.6699.55210.241228.242
ENN A1[M+NH4]+16.5685.55210.238228.239
ENN B[M+NH4]+16.3657.35196.139214.241
ENN B1[M+NH4]+16.4671.55196.140210.138
Equi[M+H]+16.7374.121175.123200.023
EGCN[M+H]+10.2562.310268.236305.234
EGCNN[M+H]+11.8562.340544.126277.238
EGST[M+H]+12.5610.340268.140223.140
EGSTN[M+H]+13.2610.35592.322268.233
EGPT[M+H]+12.0576.420223.040304.940
EGPTN[M+H]+13.0576.430558.221305.338223.049
EGSN[M+H]+9.7548.230530.020268.120223.220277.420
FB1[M+H]+14.3722.4150334.355352.349
FB2[M+H]+15.0706.4150336.349318.352
FB3[M+H]+14.7706.6140336.347688.547
Fum[M+H]+15.7459.2140131.042177.025
FuX[M+H]+6.2355.160247.030229.120
Glio[M+H]+10.7327.15263.215244.823
Grise[M+H]+13.6353.150215.025163.025285.125
HT-2[M+H]+13.7442.250263.117215.119
Lyser[M+H]+4.0268.464208.131223.227
Melea[M+H]+10.7434.25403.322334.130289.140
Mono[M+H]+13.8309.147223.1z22291.121273.321
MPA[M+H]+14.5321.347207.125302.912
NEO[M+NH4]+6.4400.110185.026215.025
O-m-Ster[M+H]+14.6339.010278.142295.038306.138324.132
Ostre A[M+NH4]+14.0543.3140508.224355.332337.336
OTA[M+H]+15.2404.1100239.034102.193358.016221.043193.050
OTB[M+H]+14.5370.060205.247324.418103.070
OTC[M+H]+15.9432.110239.137358.023386.016341.130
Oxa[M+H]+10.8448.15348.132332.138
Pse A[M+H]+9.9432.420316.214348.17
Puro[M+H]+7.4472.310309.327164.036150.136
Rq C[M+H]+13.7390.140193.235322.235
Secal A[M+H]+15.8639.2180561.228589.028579.230
Ster[M+H]+15.2325.140281.148310.135254.047
T2-triol[M+NH4]+6.4400.240214.920233.312281.312263.015
T-2[M+NH4]+14.5484.350305.119245.118215.126
Ten[M+H]+14.0415.2175312.329256.145
Wor-man[M+H]+15.5429.545355.114295.231
α-ZAL[M−H]−10.3321.1−180259.1−30303.1−28161.0−37
α-ZEL[M−H]−10.5319.1−145173.8−34160.0−42130.0−42188.1−36
β-ZAL[M−H]−9.6321.1−138303.1−31259.1−32160.9−38189.1−37
β-ZEL[M−H]−9.7319.1−134187.9−35160.1−40174.0−33
PAT[M−H]−4.6153.0−5109.0−1081.0−1683.0−19125.0−13
ZAN[M−H]−12.3319.1−148205.3−29161.1−37137.1−36177.1−36187.1−30
ZEN[M−H]−12.4317.1−90187.0−36174.9−32131.2−38
AME[M−H]−12.5271.0−160255.9−30228.0−38213.0−48183.1−54
AOH[M−H]−9.5257.0−180213.0−31215.0−32147.0−42212.0−38
Table 3. Exposure of detected mycotoxins in four matrices for Chinese people.
Table 3. Exposure of detected mycotoxins in four matrices for Chinese people.
MycotoxinExposure (ng·kg−1 b.w.day−1)PMTDI a
(ng·kg−1 b.w.day−1)		BDML10 b
(ng·kg−1 b.w.day−1)
LSLRCPCS
AFB1103.70000.23/400
AFB27.55000.09/400
AFG11.40000/400
AFG20.09000/400
AFM13.19000/4000
AFM20.54000/4000
Ster0.26000.05/160,000
OTA00.0600.0916/
DON00.9907.451000/
FB100.40015.002000/
FB200.2803.732000/
FB30000.792000/
ZEN0.420.90012.20500/
ZAN0001.30500/
BEA1.231.380.144.842000/
CIT3.170.2000200/
CPA468.61000.422000/
ENNs1.120.810.3102000/
MPA0.930.471.010.282000/
a Provisional maximum tolerable daily intake (PMTDI) established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). b BMDL10 was the benchmark dose lower confidence limit of 10% extra risk.
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Huang, X.; Feng, R.; Hu, Q.; Mao, X.; Zhou, H. Contamination Status and Health Risk Assessment of 73 Mycotoxins in Four Edible and Medicinal Plants Using an Optimized QuEChERS Pretreatment Coupled with LC-MS/MS. Toxins 2025, 17, 52. https://doi.org/10.3390/toxins17020052

AMA Style

Huang X, Feng R, Hu Q, Mao X, Zhou H. Contamination Status and Health Risk Assessment of 73 Mycotoxins in Four Edible and Medicinal Plants Using an Optimized QuEChERS Pretreatment Coupled with LC-MS/MS. Toxins. 2025; 17(2):52. https://doi.org/10.3390/toxins17020052

Chicago/Turabian Style

Huang, Xiaojing, Rui Feng, Qing Hu, Xiuhong Mao, and Heng Zhou. 2025. "Contamination Status and Health Risk Assessment of 73 Mycotoxins in Four Edible and Medicinal Plants Using an Optimized QuEChERS Pretreatment Coupled with LC-MS/MS" Toxins 17, no. 2: 52. https://doi.org/10.3390/toxins17020052

APA Style

Huang, X., Feng, R., Hu, Q., Mao, X., & Zhou, H. (2025). Contamination Status and Health Risk Assessment of 73 Mycotoxins in Four Edible and Medicinal Plants Using an Optimized QuEChERS Pretreatment Coupled with LC-MS/MS. Toxins, 17(2), 52. https://doi.org/10.3390/toxins17020052

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