Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS

Matsuo, Yosuke; Takahara, Kentaro; Sago, Yuki; Kushiro, Masayo; Nagashima, Hitoshi; Nakagawa, Hiroyuki

doi:10.3390/toxins7093700

Open AccessArticle

Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS

by

Yosuke Matsuo

¹,

Kentaro Takahara

²,

Yuki Sago

¹,

Masayo Kushiro

¹,

Hitoshi Nagashima

¹ and

Hiroyuki Nakagawa

^1,*

¹

National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan

²

Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan

^*

Author to whom correspondence should be addressed.

Toxins 2015, 7(9), 3700-3714; https://doi.org/10.3390/toxins7093700

Submission received: 30 June 2015 / Revised: 3 September 2015 / Accepted: 7 September 2015 / Published: 16 September 2015

(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)

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Abstract

:

The existence of glucose conjugates of fumonisin B₂ (FB₂) and fumonisin B₃ (FB₃) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB₂ and FB₃ bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B₂ (NDfrc-FB₂) and N-(1-deoxy-d-fructos-1-yl) fumonisin B₃ (NDfrc-FB₃) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB₂ and NDfrc-FB₃ with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB₂ and FB₃ molecules occurred to their primary amine residues.

Keywords:

LC-Orbitrap MS; fumonisin; Fusarium; corn; N-(1-deoxy-d-fructos-1-yl) fumonisin

Graphical Abstract

1. Introduction

Fusarium fungi are known as plant pathogen infecting cereals such as wheat, barley, and corn, and some of these fungi produce mycotoxins (e.g., trichothecenes, zearalenone, and fumonisins) [1]. In Japan, Fusarium fungi infection is occasionally serious, as these crops are usually planted through the rainy season. Among Fusarium mycotoxins, fumonisins are a group of naturally-occurring mycotoxins which are typically produced by Fusarium verticillioides and F. proliferatum [2]. The most abundant fumonisin is fumonisin B₁ (FB₁), followed by fumonisin B₂ (FB₂), and fumonisin B₃ (FB₃). FB₁ is a causative compound of equine leukoencephalomalacia [3] and porcine pulmonary oedema syndrome [4], and has also been confirmed to be hepatotoxic and hepatocarcinogenic in rats and mice [5,6]. Fumonisins are widely distributed geographically, and their natural occurrence in maize has been reported in various regions throughout the world [6]. A particular concern regarding fumonisins involves the higher concentrations occasionally found in maize produced and consumed by some subpopulations, such as subsistence farmers [6]. Considerable annual variations in contamination have been noted. Fumonisins also occur infrequently in other foods, including sorghum, asparagus, rice beer, and mung beans. In 2002, the FAO/WHO Joint Expert Committee on Food Additives (JECFA) established a provisional maximum tolerable daily intake (PMTDI) as 2 μg kg⁻¹ bw day⁻¹ for FB₁, FB₂, and FB₃, either alone or in combination [6]. The European Committee concluded the establishment of a group PMTDI of 2 μg kg⁻¹ bw day⁻¹ for FB₁, FB₂, and FB₃, combined [7].

Recently, a glucosylated derivative of deoxynivalenol (DON), DON-3-glucoside (DON3Glc) was found in cereal grain and beer [8,9], and similar compounds have also been found for several other mycotoxins [10,11]. Because these glucosylated derivatives are not detected by conventional analytical methods due to their higher polarity [12,13], they are referred to as “masked (modified) mycotoxins”. Hydrolysis of masked mycotoxins to their aglycons has also been reported [14,15], and it has been suggested that they present an additional potential risk to consumers. In the case of fumonisins, the presence of “bound-fumonisin” has been suggested by several researchers [16,17]. For instance, N-(1-deoxy-d-fructos-1-yl) fumonisin B₁ (NDfrc-FB₁) was found in corn, and was reportedly formed through a d-glucose binding reaction to the primary amine residue of the FB₁ molecule [18]. Although proven under laboratory conditions, the NDfrc-FB₁ occurrence at significant levels in processed samples is still controversial [19]. In addition, in vivo stability of this conjugate has not yet been definitively proven [17]. In order to understand the total fumonisin in foods and feeds correctly, there is a need to clarify the presence of these series of fumonisin conjugates. In this paper, authors report the existence of new glucose conjugates derived from type B fumonisins (FB₂ and FB₃) (Figure 1). These species were detected and identified via high resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS), in combination with treatment using a specific reagent (o-phthalaldehyde, OPA).

Figure 1. Chemical structures of FBs and their glucose conjugates.

2. Materials and Methods

2.1. Chemicals

FB₁ and FB₂ were purchased from Wako pure chemical Industries Ltd. (Osaka, Japan). FB₃ was purchased from PROMEC (Tygerberg, South Africa). All other chemicals used were commercially available and of a chemically pure grade. OPA and D-glucose (>98% of chemically pure grade) were obtained from Wako. Acetonitrile (LCMS grade) was from Fisher Scientific (Waltham, MA, USA), and distilled water (LCMS grade) was obtained from Kanto Chemical (Tokyo, Japan). Ammonium acetate (chemically pure grade) was from Kanto, and acetic acid (>99.9% of chemically pure grade, not glacial) was from Wako.

2.2. Corn Powder Sample Contaminated with Fumonisins

Mycotoxin reference material of corn powder (batch number MTC-9999C) was purchased from Trilogy Co. Ltd (Washington, MO, USA). This material was contaminated with FB₁, FB₂, FB₃, aflatoxin B₁, aflatoxin B₂, aflatoxin G₁, DON, zearalenone, HT-2 toxin, and T-2 toxin. The origin of this corn was from the USA. This was a crop naturally contaminated with the above toxins. There was no inoculation of any of the toxins in this sample. The manufacturer-warranted concentrations of FB₁, FB₂, and FB₃ were 19.8 ± 4.8 mg·kg⁻¹ (FB₁), 6.6 ± 2.1 mg·kg⁻¹ (FB₂), and 2.2 ± 1.6 mg·kg⁻¹ (FB₃), respectively. This material was stored at −20 °C in the dark until analysis.

2.3. Preparation of Stock and Working Solutions

FB₁ and FB₂ obtained as powder were accurately weighed on an aluminum boat on a micro scale, placed in a 10 mL brown volumetric flask, and dissolved in acetonitrile/water (1:1, v/v). FB₃, obtained as a crystalline sample, was directly dissolved in acetonitrile/water (1:1, v/v). The concentration was normally adjusted to 100–200 mg·L⁻¹, and stored in brown glass containers at 4 °C as the stock solutions. For preparation of the working solutions, each stock solution was taken in brown volumetric flasks, diluted appropriately with acetonitrile/water/acetic acid (5:94:1, v/v/v), and stored at 4 °C.

2.4. Extraction and Purification of Mycotoxins

Corn powder (8 g), 40 mL of methanol/water (75:25, v/v), and 0.4 mL of acetic acid (>99.9%) were homogenized with a POLYTRON PT3100 homogenizer (Kinematica AG., Lucerne, Switzerland) at a rate of 7000 rpm for 5 min, and centrifuged at 2000× g for 10 min. A portion of the supernatant (5 mL) was loaded onto a strong anion exchange column (Sep-Pak Accell Plus QMA Short Cartridge (360 mg), Waters, Milford, MA, USA) with no conditioning. Then 5 mL methanol/water (3:1, v/v) and 5 mL methanol were successively loaded on the column for washing. The FBs were eluted with 5 mL of a solution methanol/acetic acid (98:2, v/v). All of the eluent was collected in a glass tube, and the solvent was evaporated under a nitrogen gas stream at 50 °C. The residue was dissolved in 0.25 mL of acetonitrile/water/acetic acid (5:94:1, v/v/v) and subjected to LC-MS analysis.

2.5. Synthesis of N-(1-deoxy-d-fructos-1-yl) Fumonisins (NDfrc-FBs)

NDfrc-FBs was chemically prepared essentially following the procedure reported by Poling et al. [18]. In a glassware amber vial (2 mL volume), 0.1 mg of each fumonisin (stock solution of each was appropriately taken and evaporated), 40 mg of d-glucose, five or six beads of molecular sieve (pore size; 0.4 nm) (Millipore, Darmstadt, Germany), and 2 mL of methanol were taken, mixed, and further heated in an incubator with shaking (120 rpm) at 60 °C overnight. After the reaction, the solvent was evaporated under a nitrogen gas stream at 40 °C. The residue was re-dissolved in 2.5 mL of acetonitrile/water/acetic acid (5/94/1, v/v/v), and cleaned using a solid phase extraction column according to the reported procedure, with a slight modification. The re-dissolved residue (2.5 mL) was loaded on an OASIS HLB (3 cc) column (Waters), that was conditioned in advance with 3 mL of methanol and 3 mL of acetonitrile/water/acetic acid (5/94/1, v/v/v), successively. The column was washed with 6 mL of acetonitrile/water/acetic acid (5/94/1, v/v/v), and further eluted with 3 mL of methanol. This eluate was collected and evaporated to dryness under nitrogen gas at 40 °C, re-dissolved in 0.25 mL of acetonitrile/water/acetic acid (5/94/1, v/v/v), and subjected to LC-MS analysis.

2.6. LC-MS Analysis

Detection and identification of FB-glucose conjugate was conducted in accordance with author’s previous studies with the LC-Orbitrap MS instrument, “Exactive” (Thermo Fisher Scientific) [20]. LC was performed by using 0.5 mM ammonium acetate and 0.1% acetic acid aqueous solution as solvent A and 0.1% acetic acid in acetonitrile as solvent B [11]. The gradient profile used was 10% B (0–3.0 min), 90% B (18.0–22.0 min), and 10% B (22.1–29.0 min). The flow rate was set to 0.3 mL/min and the column temperature was maintained at 40 °C. The chromatographic separation was carried out on a HyPURITY C18 column (250 × 3 mm i.d., 5 μm particle size) (Thermo Fisher) with an injection volume of 0.02 mL. The Exactive mass spectrometer was operated in negative mode with a heated electrospray ionization source (HESI-II) and a spray voltage of 4.50 kV. As a typical and common fragment ion of fumonisins, ketene form of tricarballylic acid (TCA) ion [TCA−H₂O−H]⁻ (TCAK ion [TCAK−H]⁻) was detected with higher sensitivity in negative mode than positive mode. The ion of [TCAK−H]⁻ is often selected as the primary fragment for the detection of fumonisins. The capillary and the heater temperature was 350 °C and 300 °C, respectively. The sheath gas and the auxiliary gas flow rate was adjusted as 40 and 5 (in arbitrary units), respectively. The system was operated in the range of 150–1100 m/z at a resolving power of 100,000 FWHM (full width at half maximum) (m/z 200) with an accurate mass/high resolution (AM/HR) full scan (scan event 1) and all ion fragmentation spectrum acquisition with collision energy in a single run. Fragmentation was achieved with optional CID (collision-induced dissociation) equipment, using a collision energy of 60 eV (scan event 2), that was optimized with the chemical standard of FB₁. The external mass axis calibration without the use of specific lock mass was employed. For the mass accuracy estimation, the mass value observed as an abundant ion extracted at the apex of the chromatographic peak was used. The exact mass values (calculated and observed) of the analysts’ ions are summarized in Table 1, Table 2 and Table 3. The mass deviation is expressed either in terms of millimass units (mmu) or parts per million (ppm). The latter is calculated with the equation: ppm = 10⁶ × Δm/m; where Δm is the difference between theoretical (calculated) and observed mass, and m is the mass. In accordance with the European Commission guideline [21], mass deviation < 5 ppm from the calculated value was used as the criterion for compound identification. LC-Orbitrap MS is a special type of ion trap [22], and achieves a mass resolving power of up to 100,000 FWHM (m/z 200) and maintains mass accuracy (<5 ppm) even without the use of continuous internal mass correction. Therefore, it can detect and identify the various chemical compounds based on their accurate mass values calculated from the corresponding compositional formula even if those chemical standards are not available.

Table 1. Exact mass values of fumonisin B₁ (FB₁) and fumonisin B₂(NDfrc-FB₁) and relative fragment ions (calculated and observed) at negative polarity.

**Table 1.** Exact mass values of fumonisin B₁ (FB₁) and fumonisin B₂(NDfrc-FB₁) and relative fragment ions (calculated and observed) at negative polarity.
Ion	FB₁ (RT: 15.09 min)				NDfrc-FB₁ (RT: 14.87 min)
Ion	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))
[TCA-H₂O−H]⁻ ([TCAK−H]⁻)	C₆H₆O₅	157.0142	157.0137 ^c	−0.59 (−3.76)	C₆H₆O₅	157.0142	157.0140 ^c	−0.28 (−1.81)
[TCA-H₂O−H]⁻ ([TCAK−H]⁻)	C₆H₆O₅	157.0142	157.0135	−0.70 (−4.44)	C₆H₆O₅	157.0142	157.0138	−0.41 (−2.59)
[M−Glc−2TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₅	404.3381	404.3378 ^c	−0.32 (−0.78)
[M−Glc−2TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₅	404.3381	404.3383	0.20 (0.50)
[M−2TCAK−H]⁻	C₂₂H₄₇NO₅	404.3381	404.3379 ^c	−0.29 (−0.71)	C₂₂H₅₁NO₅	408.3695	-	-
[M−2TCAK−H]⁻	C₂₂H₄₇NO₅	404.3381	404.3380	−0.16 (−0.41)	C₂₂H₅₁NO₅	408.3695	-	-
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₁₀	562.3597	562.3607 ^c	0.99 (1.67)
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₁₀	562.3597	562.3607	0.99 (1.67)
[M−TCAK−H]⁻	C₂₈H₅₃NO₁₀	562.3597	562.3597 ^c	0.01 (0.02)	C₃₄H₆₃NO₁₅	724.4125	724.4136 ^c	1.14 (1.58)
[M−TCAK−H]⁻	C₂₈H₅₃NO₁₀	562.3597	562.3600	0.32 (0.56)	C₃₄H₆₃NO₁₅	724.4125	724.4137	1.20 (1.66)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₅	720.3812	720.3824 ^c	1.19 (1.65)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₅	720.3812	720.3823	1.13 (1.57)
[M−H]⁻	C₃₄H₅₉NO₁₅	720.3812	720.3805 ^c	−0.70 (−0.97)	C₄₀H₆₉NO₂₀	882.4340	882.4349 ^c	0.92 (1.04)
[M−H]⁻	C₃₄H₅₉NO₁₅	720.3812	720.3813	0.09 (0.13)	C₄₀H₆₉NO₂₀	882.4340	882.4362	2.20 (2.50)

^a Mass values calculated based on elemental formulas; ^b Mass values detected with the all ions fragmentation with collision energy (scan event 2); ^c Mass values detected by full scan (scan event 1).

2.7. Treatment of Fumonisins and FBs-Glucose Conjugate by OPA Reagent

For confirmation of the D-glucose binding position in the fumonisin molecule structures, treatment with the OPA reagent was performed. Since OPA reacts specifically with primary amines, this reagent is often used for the derivatization of fumonisin molecules, when they are analyzed by the conventional method with HPLC-fluorescence detection [23]. The OPA reagent was composed of 8 mg of OPA, successively dissolved in 0.2 mL of methanol, 0.01 mL of 2-mercaptoethanol, and 1 mL of 100 mM sodium tetraborate aqueous solution. The reagent was freshly prepared each week and stored in brown glass containers at 4 °C for protection from light exposure. The corn powder extract (0.05 mL) was reacted with OPA reagent (0.05 mL) by mixing, and immediately subjected to LC-MS analysis.

Table 2. Exact mass values of FB₂ and NDfrc-FB₂ and relative fragment ions (calculated and observed) at negative polarity.

**Table 2.** Exact mass values of FB₂ and NDfrc-FB₂ and relative fragment ions (calculated and observed) at negative polarity.
Ion	FB₂ (RT: 16.78 min)				NDfrc-FB₂ (RT: 16.40 min)
Ion	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))
[TCAK−H]⁻	C₆H₆O₅	157.0142	157.0140 ^c	−0.28 (−1.81)	C₆H₆O₅	157.0142	157.0141 ^c	−0.16 (−1.04)
[TCAK−H]⁻	C₆H₆O₅	157.0142	157.0138	−0.44 (−2.79)	C₆H₆O₅	157.0142	157.0140	−0.27 (−1.72)
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₄	388.3432	388.3430 ^c	−0.22 (−0.55)
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₄	388.3432	388.3439	0.70 (1.80)
[M−2TCAK−H]⁻	C₂₂H₄₇NO₄	388.3432	-	-	C₂₂H₅₁NO₄	392.3745	-	-
[M−2TCAK−H]⁻	C₂₂H₄₇NO₄	388.3432	388.3434 ^c	0.18 (0.47)	C₂₂H₅₁NO₄	392.3745	-	-
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₉	546.3648	546.3652 ^c	0.48 (0.88)
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₉	546.3648	546.3655	0.78 (1.44)
[M−TCAK−H]⁻	C₂₈H₅₃NO₉	546.3648	546.3650 ^c	0.23 (0.43)	C₃₄H₆₃NO₁₄	708.4176	708.4188 ^c	1.24 (1.76)
[M−TCAK−H]⁻	C₂₈H₅₃NO₉	546.3648	546.3657	0.97 (1.77)	C₃₄H₆₃NO₁₄	708.4176	708.4183	0.70 (0.98)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₄	704.3863	704.3875 ^c	1.17 (1.66)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₄	704.3863	704.3879	1.60 (2.27)
[M−H]⁻	C₃₄H₅₉NO₁₄	704.3863	704.3865 ^c	0.20 (0.28)	C₄₀H₆₉NO₁₉	866.4391	866.4410 ^c	1.88 (2.17)
[M−H]⁻	C₃₄H₅₉NO₁₄	704.3863	704.3878	1.54 (2.18)	C₄₀H₆₉NO₁₉	866.4391	866.4411	2.00 (2.31)

^a Mass values calculated based on elemental formulas; ^b Mass values detected with the all ions fragmentation with collision energy (scan event 2); ^c Mass values detected by full scan (scan event 1).

Table 3. Exact mass values of FB₃ and NDfrc-FB₃ and relative fragment ions (calculated and observed) at negative polarity.

**Table 3.** Exact mass values of FB₃ and NDfrc-FB₃ and relative fragment ions (calculated and observed) at negative polarity.
Ion	FB₃ (RT: 16.09 min)				NDfrc-FB₃ (RT: 15.68 min)
Ion	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))	Formula	Cal. Mass (m/z) ^a	Obs. Mass (m/z) ^b	Error (mmu (ppm))
[TCAK−H]⁻	C₆H₆O₅	157.0142	157.0139 ^c	−0.33 (−2.11)	C₆H₆O₅	157.0142	157.0142 ^c	−0.03 (−0.16)
[TCAK−H]⁻	C₆H₆O₅	157.0142	157.0139	−0.38 (−2.40)	C₆H₆O₅	157.0142	157.0143	0.02 (0.13)
[M−Glc−2TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₄	388.3432	388.3437 ^c	0.49 (1.25)
[M−Glc−2TCAK−H]⁻	-	-	-	-	C₂₂H₄₇NO₄	388.3432	388.3444	1.13 (2.91)
[M−2TCAK−H]⁻	C₂₂H₄₇NO₄	388.3432	-	-	C₂₂H₅₁NO₄	392.3745	-	-
[M−2TCAK−H]⁻	C₂₂H₄₇NO₄	388.3432	388.3430 ^c	−0.21 (−0.55)	C₂₂H₅₁NO₄	392.3745	-	-
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₉	546.3648	546.3655 ^c	0.78 (1.44)
[M−Glc−TCAK−H]⁻	-	-	-	-	C₂₈H₅₃NO₉	546.3648	546.3652	0.42 (0.77)
[M−TCAK−H]⁻	C₂₈H₅₃NO₉	546.3648	546.3657 ^c	0.97 (1.77)	C₃₄H₆₃NO₁₄	708.4176	708.4200 ^c	2.40 (3.39)
[M−TCAK−H]⁻	C₂₈H₅₃NO₉	546.3648	546.3658	1.03 (1.88)	C₃₄H₆₃NO₁₄	708.4176	708.4192	1.61 (2.27)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₄	704.3863	704.3880 ^c	1.72 (2.44)
[M−Glc−H]⁻	-	-	-	-	C₃₄H₅₉NO₁₄	704.3863	704.3887	2.39 (3.40)
[M−H]⁻	C₃₄H₅₉NO₁₄	704.3863	704.3871 ^c	0.81 (1.14)	C₄₀H₆₉NO₁₉	866.4391	866.4417 ^c	2.55 (2.94)
[M−H]⁻	C₃₄H₅₉NO₁₄	704.3863	704.3879	1.66 (2.36)	C₄₀H₆₉NO₁₉	866.4391	866.4420	2.91 (3.36)

^a Mass values calculated based on elemental formulas; ^b Mass values detected with the all ions fragmentation with collision energy (scan event 2); ^c Mass values detected by full scan (scan event 1).

3. Results

3.1. Detection of FB₁ and NDfrc-FB₁

Authors first confirmed the existence of FB₁ and NDfrc-FB₁ in the corn powder extract, based on the full scan results using the calculated masses. In the full scan data (scan event 1), peaks corresponding to the monitor ions [FB₁−H]⁻ (720.3812) and [NDfrc-FB₁−H]⁻ (882.4340) were detected at 15.09 min and 14.87 min, respectively. The same peaks were observed when standard FB₁ and authentic NDfrc-FB₁ were injected into the LC-MS system. Regarding detection of NDfrc-FB₁, abundant [NDfrc-FB₁−H]⁻ (882.4349) ion was detected with a deviation of 0.92 mmu (1.04 ppm) (Figure 2B). In addition, the fragment ions [NDfrc-FB₁−TCAK−H]⁻ (724.4136), [NDfrc-FB₁−Glc−H]⁻ (720.3824), [NDfrc-FB₁−Glc−TCAK−H]⁻ (562.3607), and [NDfrc-FB₁−Glc−2TCAK−H]⁻ (404.3378) were observed, with deviations of 1.14 mmu (1.58 ppm), 1.19 mmu (1.65 ppm), 0.99 mmu (1.67 ppm) and −0.32 mmu (−0.78 ppm), respectively (Figure 2B,C). During the scan event 2, the latter two fragment ions, as well as [TCAK−H]⁻ (157.0138) provided dual peaks (at 15.11 min and 14.88 min) (Figure 2A, Table 1), indicating that similar fragmentation was occurring for FB₁ and NDfrc-FB₁. Although [NDfrc-FB₁−2TCAK−H]⁻ (408.3695) was suggested as a fragment of NDfrc-FB₁ (Table 1), a corresponding ion was not detected for NDfrc-FB₁ (chemically synthesized or contained in corn extract). The observed mass values and their respective mass deviations from the calculated values are summarized in Table 1.

3.2. Detection and Identification of NDfrc-FB₂ and NDfrc-FB₃

Figure 3 shows the results of screening for NDfrc-FB₂ and NDfrc-FB₃ in the corn powder extract. Using the same procedure as adopted for NDfrc-FB₁, the existence of FB₂ and FB₃ was first confirmed based on the full scan results (scan event 1) using the calculated mass of [FB₂−H]⁻ (704.3863). A major peak corresponding to [FB₂−H]⁻ was detected at 16.78 min, as shown at the top of Figure 3A, and the same peak was found for the FB₂ standard. As fragment ions of FB₂, [FB₂−TCAK−H]⁻, [FB₂−2TCAK−H]⁻, and [TCAK−H]⁻ were observed (Table 2). When the full-scan results (scan event 1) were scrutinised with the calculated mass of [NDfrc-FB₂−H]⁻ (866.4391), a major peak was detected for [NDfrc-FB₂−H]⁻ at 16.40 min (Figure 3A), and abundant [NDfrc-FB₂–H]⁻ (866.4410) was detected with a mass deviation of 1.88 mmu (2.17 ppm) (Figure 3B). In addition, the fragment ions [NDfrc-FB₂–TCAK−H]⁻ (708.4188), [NDfrc-FB₂−Glc−H]⁻ (704.3875), [NDfrc-FB₂−Glc−TCAK−H]⁻ (546.3652), [NDfrc-FB₂−Glc−2TCAK−H]⁻ (388.3430) were observed with deviations of 1.24 mmu (1.76 ppm), 1.17 mmu (1.66 ppm), 0.48 mmu (0.88 ppm), and −0.22 mmu (−0.55 ppm), respectively (Figure 3B, Table 2). Due to the low intensities of the signals, several fragment ions (Figure 3C) were observed only in the magnified spectra. Two peaks at 16.79 min and 16.39 min were detected for the monitor ions [NDfrc-FB₂−Glc−TCAK−H]⁻ (546.3648) and [NDfrc-FB₂−Glc−2TCAK−H]⁻ (388.3432) with scan event 2 (Figure 3A), suggesting that similar fragmentation was occurring for FB₂ and NDfrc-FB₂. During scan event 2, fragments corresponding to [NDfrc-FB₂−TCAK−H]⁻ and [NDfrc-FB₂−Glc−2TCAK−H]⁻ were observed (Table 2). The respective mass values of these fragments were 708.4183 mmu and 388.3439 mmu, with mass deviations from the calculated values of 0.70 mmu (0.98 ppm) and 0.70 mmu (1.80 ppm), respectively. In the case of screening for NDfrc-FB₃, a major peak for [NDfrc-FB₃−H]⁻ was observed at 15.68 min (scan event 1) (Figure 3A), and a fragmentation pattern similar to that of NDfrc-FB₂ was also confirmed (details shown in Table 3). There was no difference in the fragmentation patterns of FB₂ and FB₃, as [FB₃−TCAK−H]⁻, [FB₃−2TCAK−H]⁻, and [TCAK−H]⁻ were observed as the corresponding fragment ions. Based on the data described above, authors were convinced that both NDfrc-FB₂ and NDfrc-FB₃ were contained in the corn powder extract.

Figure 2. Detection and identification of NDfrc-FB₁. Mass chromatogram with scan results (scan events 1 and 2) (A); full mass spectrum obtained at 14.87 min (scan event 1) (B); and mass range magnification of full mass spectrum (m/z: 400–410) obtained at 14.87 min (scan event 1) (C).

Figure 3. Detection and identification of NDfrc-FB₂. Mass chromatogram with scan results (scan events 1 and 2) (A); full mass spectrum obtained at 16.40 min (scan event 1) (B); and mass range magnification of full mass spectrum (m/z: 385–395) obtained at 16.40 min (scan event 1) (C).

3.3. Structures of NDfrc-FB₂ and NDfrc-FB₃

Figure 4 shows the LC-MS chromatograms of the NDfrc-FB₂ (NDfrc-FB₃) and FB₂ (FB₃) detected in the corn powder extract before and after treatment with OPA reagent (scan event 1). If the structures of NDfrc-FB₂ and NDfrc-FB₃ were similar to that of NDfrc-FB₁ (glucose bound to the primary amine of the fumonisin molecule), it was considered that OPA would not react with these species. As shown in Figure 4, the signal intensities of FB₂ and FB₃ were decreased by the OPA treatment, whereas those of NDfrc-FB₂ and NDfrc-FB₃ were not. The peak area ratios of NDfrc-FB₂ (NDfrc-FB₃), before to after the OPA reaction, were 1.44–1.46. On the other hand, these ratios of FB₂ (FB₃), before to after the OPA reaction were 281.4–1372.1. These results indicate that the primary amine residue was occupied by glucose conjugation in the molecules of NDfrc-FB₂ (NDfrc-FB₃). The slight shift observed for the fumonisin peaks was attributed to the increase in methanol concentration in the samples following the OPA treatment.

Figure 4. Chromatograms of NDfrc-FB₂ (NDfrc-FB₃) and FB₂ (FB₃) in corn powder extract before and after the treatment with OPA reagent (scan event 1).

In order to confirm the retention time and fragmentation profiles of NDfrc-FB₂ and NDfrc-FB₃ during the LC-Orbitrap MS analysis, these compounds were chemically synthesized with the standards of FB₂ and FB₃ with reference to the Poling et al. [18] report. Figure 5 shows the chromatograms of NDfrc-FB₂ and NDfrc-FB₃ in the corn powder extract and for chemically synthesized species. The mass fragmentation profiles of the synthesized NDfrc-FB₂ (NDfrc-FB₃) were in agreement with those of NDfrc-FB₂ (NDfrc-FB₃) detected in the corn sample extract (Figure S1). These results indicate that NDfrc-FB2 (and NDfrc-FB₃) appear to be formed though a non-enzymatic reaction between FB₂ (and FB₃) and D-glucose. In addition, these synthesized NDfrc-FB₂ and NDfrc-FB₃ did not react with the OPA reagent (details not shown). Because the synthesized NDfrc-FB₂ (and NDfrc-FB₃) were not sufficiently pure, and contained remaining FB₂ (and FB₃), they could not be used for performing the quantitative analysis.

Figure 5. Chromatograms of NDfrc-FB₂ and NDfrc-FB₃ in corn powder extract in comparison with the chemically synthesized species (scan event 1).

4. Discussion

In 2002, Poling et al. [18] reported that NDfrc-FB₁ was formed through a non-enzymatic reaction between FB₁ and glucose. Hence, authors presumed that FB₂ and FB₃ could also react with d-glucose non-enzymatically to form glucose conjugates such as NDfrc-FB₁. In the current study, the existence of FB₂-glucose and FB₃-glucose conjugates (suggested to be NDfrc-FB₂ and NDfrc-FB₃) in corn powder extract was confirmed by LC-Orbitrap MS. In order to confirm that the glucose conjugation occurred at the amine residue of the FB₁ molecule, Lu et al. [24] treated the FB₁-glucose conjugate with OPA reagent. In the same manner, NDfrc-FB₂ (and NDfrc-FB₃) was treated with OPA in the current study. When analyzed by LC-Orbitrap MS, the peaks suggested to be NDfrc-FB₂ and NDfrc-FB₃ were scarcely reduced after OPA treatment, whereas those of FB₂ and FB₃ were reduced substantially. Based on these observations, accompanied with the high-resolution MS spectrum data described above, authors became convinced that the conjugates detected in this study should correctly correspond to NDfrc-FB₂ and NDfrc-FB₃. Additionally, NDfrc-FB₂ and NDfrc-FB₃ were observed at a different lot number of Trilogy mycotoxin reference materials (MTC-9999A and MTC-9999E) (Figure S2).

In our previous studies, several trichothecene glucosides (O-glucoside conjugates) were detected in a corn reference material sample of the same line from Trilogy Co. Ltd (although the batch number was different) [11,20,22]. Therefore, authors initially screened for the presence of O-glucoside conjugates of FB₁–FB₃. After treating the corn powder extract with the OPA reagent, the full MS scan data was scrutinized with the calculated mass values of C₅₀H₇₇NSO₂₁ (FB₁-O-glucoside-OPA) and C₅₀H₇₇NSO₂₀ (FB₂ (FB₃)-O-glucoside-OPA), respectively. However, no peaks were detected. Based on these results, it appears that fumonisins are not enzymatically glucosylated, but chemically bound to glucose in-plant, which differs from the case of trichothecenes.

Among the fumonisin isomers, there are several different groups, such as fumonisin A (FA) [25] and fumonisin C (FC) [26] in addition to fumonisin B (FB₁–FB₃). It is suggested that FA hardly reacts with d-glucose, because its amine residue is acetylated. In contrast, FC appears to react with d-glucose non-enzymatically via the free primary amine harbored in its structure. NDfrc-FB₁ was found from the cooked maize with heat [27], whereas NDfrc-FB₁, NDfrc-FB₂, and NDfrc-FB₃ were found in the corn powder (not cooked sample) used in this study. These conjugates appeared to be formed through a non-enzymatic reaction between fumonisins (FB₂ and FB₃) and glucose, as reported for FB₁ [18]. Therefore, it seems likely that high temperature (around the cooking conditions) is not indispensable for the formation of NDfrc-FBs. Once the corn grains are harvested, they are normally dried, stored at the keeping place, and ground if necessary. In the drying process, if the corn grains containing fumonisins were subjected to some heat (for promoting to eliminate the moisture), NDfrc-FB might be formed. From the standpoint of toxicity, NDfrc-FB₁ has been reported as less toxic, compared to FB₁ [28]. The toxicity of NDfrc-FB₂ and NDfrc-FB₃ is suggested to be lower than FB₂ and FB₃ with reference to NDfrc-FB₁. It was also reported that NDfrc-FB₁ was partly converted back to FB₁ in the gastrointestinal tract of rats [29]. On the other hand, Cirlini et al. [30] reported that NDfrc-FB₁ was not reduced to FB₁ in vitro digestion model [30]. As one possible factor reducing NDfrc-FB₁ to FB₁, the involvement of rat microbiota is inferred. Although crucial microbes reducing NDfrc-FB₁ are unknown, the microbiota in the gastrointestinal tract is greatly different amongst host species, area, and age [31,32]. Another important question concerns the amount of these fumonisin conjugates that are present. However, in the current study, it was not possible to estimate the amounts of these compounds due to a lack in the pure (purified) chemical standards.

5. Conclusions

In conclusion, new glucose conjugates of FB₂ and FB₃ (NDfrc-FB₂ and NDfrc-FB₃) were detected for the first time in the corn sample in this study. These conjugates appeared to be formed through a non-enzymatic reaction between fumonisins (FB₂ and FB₃) and glucose. Although these reactions are similar to Maillard reaction, NDfrc-FBs are not reduced to FBs in contrast to the conjugate reaction of amino acid and D-glucose [33] even if they are treated with alkali. Considering that NDfrc-FB seems to be less toxic than FB, some food processing procedures (for example, promotion of Maillard reaction) can be suggested to mitigate the fumonisin toxicity, as examined previously [19]. The existence of acyl-fumonisin B₁ [17] and fumonisins bound to starch (hidden fumonisins) [34] has also been reported by other researchers. Although the use of the hydrolyzed FBs (HFBs) obtained with either alkaline or enzymatic treatment has been proposed for quantitation of the total fumonisins in starch [17], NDfrc-FBs should not be determined with these methods. Since the PMTDI value was designated as 2 μg·kg⁻¹ bw·day⁻¹ in the JECFA [6], the existence of those fumonisin conjugates may be taken into account for the establishment of this value. In order to estimate the potential risk of fumonisins, further studies on the prevalence of these and other conjugates in foods, as well as their relevance for human health is needed.

Supplementary Materials

Supplementary materials can be accessed at: https://www.mdpi.com/2072-6651/7/9/3700/s1.

Acknowledgments

A part of this work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (research project for improving food safety and animal health).

Author Contributions

Yosuke Matsuo, Hiroyuki Nakagawa, and Masayo Kushiro conceived and designed the experiments. Yosuke Matsuo and Yuki Sago prepared all kinds of samples from the crop extract, and Masayo Kushiro administrated the OPA treatment experiments for the structure determination of fumonisin conjugates. Yosuke Matsuo, Hiroyuki Nakagawa, and Kentaro Takahara conducted the LC-Orbitrap MS experiments and analyzed the data. Yosuke Matsuo and Hiroyuki Nakagawa mainly constructed the manuscript under the support of all co-authors. Masayo Kushiro, Hitoshi Nagashima and Hiroyuki Nakagawa supervised the work and revised the manuscript for important intellectual content.

Conflicts of Interest

The authors declare no conflicts of interest.

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Matsuo, Y.; Takahara, K.; Sago, Y.; Kushiro, M.; Nagashima, H.; Nakagawa, H. Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS. Toxins 2015, 7, 3700-3714. https://doi.org/10.3390/toxins7093700

AMA Style

Matsuo Y, Takahara K, Sago Y, Kushiro M, Nagashima H, Nakagawa H. Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS. Toxins. 2015; 7(9):3700-3714. https://doi.org/10.3390/toxins7093700

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Matsuo, Yosuke, Kentaro Takahara, Yuki Sago, Masayo Kushiro, Hitoshi Nagashima, and Hiroyuki Nakagawa. 2015. "Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS" Toxins 7, no. 9: 3700-3714. https://doi.org/10.3390/toxins7093700

APA Style

Matsuo, Y., Takahara, K., Sago, Y., Kushiro, M., Nagashima, H., & Nakagawa, H. (2015). Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS. Toxins, 7(9), 3700-3714. https://doi.org/10.3390/toxins7093700

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Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS

Abstract

1. Introduction