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Article

Loss-of-Function Mutations of BCOR Are an Independent Marker of Adverse Outcomes in Intensively Treated Patients with Acute Myeloid Leukemia

by
Jan-Niklas Eckardt
1,*,†,‡,
Sebastian Stasik
1,†,‡,
Michael Kramer
1,‡,
Christoph Röllig
1,‡,
Alwin Krämer
2,‡,
Sebastian Scholl
3,‡,
Andreas Hochhaus
3,‡,
Martina Crysandt
4,‡,
Tim H. Brümmendorf
4,‡,
Ralph Naumann
5,‡,
Björn Steffen
6,‡,
Volker Kunzmann
7,‡,
Hermann Einsele
7,‡,
Markus Schaich
8,‡,
Andreas Burchert
9,‡,
Andreas Neubauer
9,‡,
Kerstin Schäfer-Eckart
10,‡,
Christoph Schliemann
11,‡,
Stefan W. Krause
12,‡,
Regina Herbst
13,‡,
Mathias Hänel
13,‡,
Norbert Frickhofen
14,‡,
Richard Noppeney
15,‡,
Ulrich Kaiser
16,‡,
Claudia D. Baldus
17,‡,
Martin Kaufmann
18,‡,
Zdenek Rácil
19,‡,
Uwe Platzbecker
20,‡,
Wolfgang E. Berdel
11,‡,
Jiří Mayer
19,‡,
Hubert Serve
6,‡,
Carsten Müller-Tidow
2,‡,
Gerhard Ehninger
1,‡,
Friedrich Stölzel
1,‡,
Frank Kroschinsky
1,‡,
Johannes Schetelig
1,21,‡,
Martin Bornhäuser
1,22,‡,
Christian Thiede
1,‡,§ and
Jan Moritz Middeke
1,‡,§add Show full author list remove Hide full author list
1
Medizinische Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus, 01307 Dresden, Germany
2
Deutsches Krebsforschungszentrum (DKFZ) and Medizinische Klinik V, Universitätsklinikum Heidelberg, 69120 Heidelberg, Germany
3
Klinik für Innere Medizin II, Universitätsklinikum Jena, 07740 Jena, Germany
4
Klinik für Hämatologie, Onkologie, Hämostaseologie und Stammzelltransplantation, Uniklinik RWTH Aachen, 52074 Aachen, Germany
5
Medizinische Klinik III, St. Marien-Krankenhaus Siegen, 57072 Siegen, Germany
6
Medizinische Klinik II, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany
7
Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg, 97080 Würzburg, Germany
8
Klinik für Hämatologie, Onkologie und Palliativmedizin, Rems-Murr-Klinikum Winnenden, 71364 Winnenden, Germany
9
Klinik für Hämatologie, Onkologie, Immunologie, Philipps Universität, 35043 Marburg, Germany
10
Klinik für Innere Medizin V, Klinikum Nürnberg Nord, 90419 Nürnberg, Germany
11
Medizinische Klinik A, Universitätsklinikum Münster, 48149 Münster, Germany
12
Medizinische Klinik 5, Universitätsklinikum Erlangen, 91054 Erlangen, Germany
13
Medizinische Klinik III, Klinikum Chemnitz, 09116 Chemnitz, Germany
14
Innere Medizin III, HSK Wiesbaden, 65199 Wiesbaden, Germany
15
Klinik für Hämatologie, Universitätsklinikum Essen, 45147 Essen, Germany
16
Medizinische Klinik II, St. Bernward Krankenhaus, 31134 Hildesheim, Germany
17
Hämatologie und Onkologie, Charité-Universitätsmedizin, 10117 Berlin, Germany
18
Abteilung für Hämatologie, Onkologie und Palliativmedizin, Robert-Bosch-Krankenhaus, 70376 Stuttgart, Germany
19
Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital, 60177 Brno, Czech Republic
20
Medizinische Klinik und Poliklinik I, Hämatologie und Zelltherapie, Universitätsklinikum Leipzig, 04103 Leipzig, Germany
21
DKMS Clinical Trials Unit, 01309 Dresden, Germany
22
National Center for Tumor Diseases, 01307 Dresden, Germany
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
The authors belong to the Study Alliance Leukemia (SAL).
§
These authors contributed equally to this work.
Cancers 2021, 13(9), 2095; https://doi.org/10.3390/cancers13092095
Submission received: 18 March 2021 / Revised: 15 April 2021 / Accepted: 22 April 2021 / Published: 26 April 2021
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Abstract

:

Simple Summary

Acute myeloid leukemia (AML) is a genetically heterogeneous disease. Clinical phenotypes of frequent mutations and their impact on patient outcome are well established. However, the role of rare mutations often remains elusive. We retrospectively analyzed 1529 newly diagnosed and intensively treated AML patients for mutations of BCOR and BCORL1. We report a distinct co-mutational pattern that suggests a role in disease progression rather than initiation, especially affecting mechanisms of DNA-methylation. Further, we found loss-of-function mutations of BCOR to be independent markers of poor outcomes in multivariable analysis. Therefore, loss-of-function mutations of BCOR need to be considered for AML management, as they may influence risk stratification and subsequent treatment allocation.

Abstract

Acute myeloid leukemia (AML) is characterized by recurrent genetic events. The BCL6 corepressor (BCOR) and its homolog, the BCL6 corepressor-like 1 (BCORL1), have been reported to be rare but recurrent mutations in AML. Previously, smaller studies have reported conflicting results regarding impacts on outcomes. Here, we retrospectively analyzed a large cohort of 1529 patients with newly diagnosed and intensively treated AML. BCOR and BCORL1 mutations were found in 71 (4.6%) and 53 patients (3.5%), respectively. Frequently co-mutated genes were DNTM3A, TET2 and RUNX1. Mutated BCORL1 and loss-of-function mutations of BCOR were significantly more common in the ELN2017 intermediate-risk group. Patients harboring loss-of-function mutations of BCOR had a significantly reduced median event-free survival (HR = 1.464 (95%-Confidence Interval (CI): 1.005–2.134), p = 0.047), relapse-free survival (HR = 1.904 (95%-CI: 1.163–3.117), p = 0.01), and trend for reduced overall survival (HR = 1.495 (95%-CI: 0.990–2.258), p = 0.056) in multivariable analysis. Our study establishes a novel role for loss-of-function mutations of BCOR regarding risk stratification in AML, which may influence treatment allocation.

1. Introduction

Acute myeloid leukemia (AML) is a genetically heterogeneous disease [1]. In the last decade, next-generation sequencing (NGS) has been introduced in hematological practice, and with the use of myeloid gene panels, rare and recurrent mutations have been unveiled, with a majority of patients harboring more than one mutation even within defined AML entities [2]. The discovery of common recurrent mutations, such as NPM1 and FLT3-ITD, among others, has led to a better understanding of the molecular landscape of AML [1,3], with distinct consequences for prognostication and treatment allocation [4]. However, the role and potential impact of rare mutations on prognosis is not well understood, and warrants further studies to improve risk assessment, implying a more precise approach to AML treatment, as relapse and mortality rates are still unsatisfactory.
The BCL6 corepressor (BCOR) gene, and its homolog, the BCL6 corepressor-like 1 (BCORL1) gene, are located on chromosomes Xp11.4 and Xq26.1, respectively [5,6]. BCOR was originally described as a repressor of BCL6 [5], but also interacts with PCGF1, KDM2B, MLLT3, and IRF8, while BCORL1 interacts with PCGF1, KDM2B, CTBP1, and HDAC [7]. Both BCOR and BCORL1 are core proteins of the polycomb repressive complex PRC1.1. PRCs are essential for the maintenance of cell identity and cell differentiation, and their perturbance is an important factor in carcinogenesis [8]. PRC1.1 plays a role in epigenetic modification by adding an ubiquitin to histone H2A at lysine 119 [9]. This process (among others) leads to a silencing of Hox gene clusters [10], and mediates transcriptional repression [11]. BCOR is highly expressed in embryonic stem cells, where a role in maintaining the primed pluripotent state has been suggested [7,12]. As a mediator of stemness and differentiation, BCOR is involved in the development of B- and T-cells, as well as erythrocytes [13].
Since BCOR is vital in ectodermal and mesenchymal differentiation, germline loss-of-function mutations in BCOR lead to oculofaciocardiodental (OFCD) syndrome in heterozygous females, and prenatal death in hemizygous males [14]. Somatic mutations have been found in a variety of solid tumors, such as retinoblastoma, medulloblastoma, osteosarcoma, and hepatocellular carcinoma [15,16,17,18]. BCOR is an important factor in the regulation of myeloid cell proliferation and differentiation [12]. It has been described in hematologic entities such as aplastic anemia [19,20], chronic myelomonocytic leukemia [21], clonal hematopoiesis of indeterminate potential [22], myelodysplastic syndromes (MDS) [21,23,24,25], and AML [6,21,26,27,28]. In AML, mutations of BCOR (mBCOR) and BCORL1 (mBCORL1) both occur in 4–6% of patients, and an association with poor outcomes has been suggested [6,26,27,28]. However, given the rarity of these mutations, and the confinement of previous studies to smaller patient cohorts, further investigation of their impact on clinical phenotypes and outcomes in AML seems warranted. We here present the analysis on the impact of BCOR/BCORL1 mutational status on a large cohort of newly diagnosed and intensively treated AML patients.

2. Materials and Methods

2.1. Data Set

We retrospectively analyzed a multi-center cohort of 1529 AML patients. Eligibility criteria were newly diagnosed AML according to WHO definitions [29], age ≥ 18 years, and available biomaterial at diagnosis. All patients were treated with intensive regimens in the following clinical trials: AML96 [30], AML2003 [31], AML60+ [32], and SORAML [33] or were enrolled in the German Study Alliance Leukemia (SAL)’s AML registry (NCT03188874). Detailed information on treatment regimens is given in the respective references. All mentioned studies were carried out under the auspices of the SAL, and approved by the Institutional Review Board of the Dresden University of Technology (Dresden, Saxony, Germany). All participants gave their written, informed consent, in accordance with to the Declaration of Helsinki.

2.2. Definitions

AML was defined as de novo when neither previous malignancy nor previous treatment with chemo- and/or radiotherapy was reported. When myeloid neoplasms were documented prior to AML diagnosis, AML was defined as secondary (sAML). Prior exposure to chemo- and/or radiotherapy before the initial diagnosis defined therapy-associated AML (tMN). Early death was defined as death by any cause within 30 days of the initial diagnosis (ED30). Remission and survival criteria were defined according to ELN2017 recommendations [4].

2.3. Molecular Analysis

Next generation sequencing (NGS) using a TruSight Myeloid Sequencing Panel (Illumina, San Diego, CA, USA) was performed on pre-treatment bone marrow or peripheral blood, targeting 54 genes (Table S1) associated with myeloid neoplasms, including full coding exons of BCOR and BCORL1, as previously described in detail [34,35]. A DNeasy blood and tissue kit (Qiagen, Hilden, Germany) was used to extract DNA, and subsequent quantification was performed using a NanoDrop spectrophotometer. Pooled samples were sequenced paired-end (150 bp PE) using a NextSeq NGS instrument (Illumina). The SEQUENCE PILOT software package (JSI medical systems GmbH, Ettenheim, Germany) was used for sequence data alignment, variant calling, and filtering. A 5% variant allele frequency (VAF) cut-off was used. Genome-mapping algorithms were referenced to human genome build HG19. We compared VAFs of BCOR/BCORL1 mutations with VAFs of co-mutated drivers for dichotomization of dominant, subclonal, and secondary mutations. For putative subclonal mutations a minimum VAF difference of 10% was applied.

2.4. Statistical Analysis

We compared categorical variables between groups using the chi-squared test, while continuous variables were compared using the Kruskal–Wallis test. The Kaplan–Meier method was used to estimate survival probabilities. The logrank test was used to compare survival time distributions between groups. Cox regression was used to estimate univariate and adjusted hazard ratios. For the binary endpoint of complete remission, logistic regression models were fitted to estimate univariate and adjusted odds ratios. A significance level of 0.05 was used to determine statistical significance. Calculations were performed in R 4.0.3.

3. Results

In the entire cohort (n = 1529), we found mBCOR in 71 (4.6%) and mBCORL1 in 53 (3.5%) patients. Twelve patients (0.8%) concomitantly harbored both mBCOR and mBCORL1. The median age for the entire cohort was 55 years (Interquartile range (IQR): 44–64). Median variant allele frequency (VAF) for mBCOR and mBCORL1 was 48% (range: 7–100%) and 47% (range: 5–100%), respectively (Figure 1A and Figure 2A). Most patients harboring mBCOR (87%) and mBCORL1 (83%) carried two or more additional mutations in other driver genes, while only one patient in each group had no other co-mutations detected by the panel (Figure 1B and Figure 2B). Both mBCOR and mBCORL1 were more frequently found in the dominant AML clone (76% and 68%, respectively, Figure 1C and Figure 2C). Mutations of BCOR were found in several exons and functional domains, including the MLLT3, ANK, and PUFD domains (Figure 1D). The majority (62%) of the mutations were single-nucleotide variants (SNVs). Most mutations had a loss-of-function (LOF) effect (frameshift or non-sense mutations, 68%, Figure 1E), likely resulting in a premature stop of transcription and inactivation of the BCOR protein. The remaining 32% of BCOR mutations exclusively comprised missense SNVs, with a presumed damaging effect for the majority of variants, as suggested by the PolyPhen-2 classifier [36].
However, due to the unknown effect of protein function, these mutations were classified as UFs (unknown functions) for sub-analysis. The co-mutational landscape of mBCOR AML (Figure 1F) was characterized by high rates of mutation in DNMT3A (39%), RUNX1 (30%), TET2 (23%), NRAS (20%), BCORL1 (17%), and STAG2 (17%). Low mutation frequencies were detected for other genes frequently mutated in AML, such as FLT3 (ITD: 10%, TKD: 3%), NPM1 (11%), and TP53 (4%). Similar to mBCOR, in mBCORL1 AML the majority of mutations had a loss-of-function effect (60%, Figure 2D) and were mostly SNVs (62%). The most common co-mutations (Figure 2E) were DNMT3A (34%), RUNX1 (34%), FLT3-ITD (25%), BCOR (23%), and TET2 (23%).
Mutations of BCORL1 were more prevalent in females than in males (mBCORL1 female 62.3%, male: 37.7%; wild-type BCORL1 female: 47.2%, male: 52.8%; p = 0.044), while for mBCOR no such association was observed. For both mBCOR and mBCORL1 no statistically significant associations were detected for age at diagnosis, the presence of a complex karyotype, hemoglobin levels, or platelet counts. With respect to disease status, the rate of sAML was significantly higher amongst patients harboring mBCOR than amongst wtBCOR patients (21.7% vs. 11%, p = 0.023), while no specific association with mutation type (LOF or UF) or mBCORL1 was found regarding AML type. Outcomes did not differ for patients with sAML and mBCOR or mBCORL1 compared to patients with de novo AML. In the ELN2017 intermediate-risk group we found a higher proportion of LOF of mBCOR compared to UF mBCOR and wtBCOR (LOF mBCOR: 86.4% vs. UF mBCOR: 28.6% vs. wtBCOR: 38.8%, p < 0.001), as well as mBCORL1 compared to wtBCORL1 (70% vs. 39.1%, p < 0.001). Median white blood cell (WBC) counts in LOF mBCOR AML were significantly lower compared to UF mBCOR and wtBCOR (p < 0.001), while mBCORL1 showed no significant association with WBC. Table 1 and Table 2 summarize patient characteristics for mBCOR (LOF and UF) and mBCORL1, respectively.
With respect to clinical outcomes, mBCOR and mBCORL1 were not associated with the rate of complete remission (CR) (mBCOR: OR = 0.781 (95%-CI: 0.469–1.301), p = 0.342 and mBCORL1: OR = 0.795 (95%-CI: 0.442–1.432), p = 0.445) or with ED30 (mBCOR: OR = 0.679 (95%-CI: 0.209–2.199), p = 0.518 and mBCORL1: OR = 1.288 (95%-CI: 0.454–3.649), p = 0.634). In contrast, mBCOR was associated with lower median measures of event-free survival (EFS) (2.8 months (95%-CI: 1.7–8.5) vs. 7.6 months (95%-CI: 6.8–8.5), HR = 1.485 (95%-CI: 1.147–1.922), p = 0.003; Figure 3A), relapse-free survival (RFS) (9.9 months (95%-CI: 8.1–24.0) vs. 18.7 months (95%-CI: 16.3–23.3), HR = 1.452 (95%-CI: 1.047–2.014), p = 0.026; Figure 3B), and overall survival (OS) (13.4 months (95%-CI: 10.4–27.4) vs. 18.5 months (95%-CI: 16.8–21.4), HR = 1.452 (95%-CI: 0.959–1.677), p = 0.095; Figure 3C) in a univariate analysis. In a multivariable model adjusted for age, AML type (de novo, sAML, tMN), and ELN2017 risk, mBCOR in general was not independently associated with EFS (HR = 1.243 (95%-CI: 0.89–1.736), p = 0.202), RFS (HR = 1.407 (95%-CI: 0.93–2.129), p = 0.106), and OS (HR = 1.216 (95%-CI: 0.846–1.748), p = 0.292).
For patients harboring LOF mBCOR, compared to UF mBCOR and wtBCOR, we found in both univariate and multivariable analysis significantly reduced median EFS (LOF mBCOR: 1.9 months (95%-CI: 1.4–8.0) vs. UF variant of mBCOR: 4.9 months (95%-CI: 1.676–n.a.) vs. wild-type BCOR: 7.5 months (95%-CI: 6.8–8.5), multivariable HR of LOF compared to wtBCOR = 1.464 (95%-CI: 1.005–2.134), p = 0.047, Figure 4A) and RFS (LOF mBCOR: 8.8 months (95%-CI: 7.3–24.0) vs. UF variant of mBCOR: 17.7 months (95%-CI: 8.317–n.a.) vs. wild-type BCOR: 18.7 months (95%-CI: 16.3–23.3), multivariable HR of LOF compared to wtBCOR = 1.904 (95%-CI: 1.163–3.117), p = 0.01, Figure 4B). Regarding OS, we found significantly reduced median OS for patients with LOF mBCOR variants in univariate analysis (LOF mBCOR: 11.6 months (95%-CI: 8.5–33.2) vs. UF variant of mBCOR: 14.2 months (95%-CI: 11.045–n.a.) vs. wild-type BCOR: 18.4 months (95%-CI: 16.7–21.4), HR of LOF compared to wtBCOR = 1.409 (95%-CI: 1.010–1.965), p = 0.044), and a strong trend for reduced median OS in a multivariable analysis (HR = 1.495 (95%-CI: 0.990–2.258), p = 0.056, Figure 4C). Additionally, adjusting for co-mutations in DNMT3A, TET2, and RUNX1 did not significantly affect HR. However, in order to precisely account for multiple interactions, an even larger cohort is needed, due to the rarity of LOF BCOR.
In AML with mBCORL1 in general compared to wtBCORL1, median EFS (3.6 months (95%-CI: 1.9–9.8) vs. 7.5 months (95%-CI: 6.7–8.3), HR = 1.204 (95%-CI: 0.885–1.639), p = 0.236; Figure 5A), median RFS (14.8 months (95%-CI 7.6–66.6) vs. 18.5 months (95%-CI: 15.9–23.3), HR = 1.263 (95%-CI: 0.84–1.897), p = 0.263; Figure 5B) and median OS (14.9 months (95%-CI: 9.2–27.4) vs. 18.5 months (95%-CI: 16.7–21.4), HR = 1.105 (95%-CI: 0.714–1.71), p = 0.654; Figure 5C) were both lower, although none of these differences were statistically significant. Univariate analysis of LOF mBCORL1 showed significantly reduced EFS (LOF mBCORL1: 3.5 months (95%-CI: 1.1–10.1) vs. UF mBCORL1: 6.2 months (95%-CI: 1.9–not reached) vs. wtBCORL1: 7.5 months (95%-CI: 6.7–8.3), HR = 1.521 (95%-CI: 1.045–2.213), p = 0.028); however, in a multivariable analysis adjusting for age, AML type, and ELN2017 risk, this was not statistically significant.
We did not find statistically significant differences in mBCOR and mBCORL1 regarding outcomes in different ELN2017 risk groups. Since few patients with mBCOR and mBCORL1 were in the ELN2017 favorable (n = 9 and n = 6, respectively) or ELN2017 adverse-risk groups (n = 12 and n = 6, respectively), we focused on the ELN2017 intermediate-risk group (n = 44 and n = 35, respectively). In ELN2017 intermediate-risk AML with mBCOR, median EFS and OS did not differ compared to wtBCOR, while there was a trend of lower median RFS in a univariate model (11.9 months (95%-CI: 7.0–24.0) vs. 14.8 months (95%-CI: 12.5–20.3), HR = 1.446 (95%-CI: 0.977–2.139), p = 0.065), as well as in a multivariable model adjusted for age and AML type (HR = 1.637 (95%-CI: 0.995–2.693), p = 0.052). In ELN2017 intermediate-risk AML with mBCORL1, median EFS and RFS did not differ, but there was a trend of lower median OS in a univariate model (14.9 months (95%-CI: 7.3–24.4) vs. 17.0 months (95%-CI: 14.0–20.6), HR = 1.401 (95%-CI: 0.954–2.058), p = 0.086), while in a multivariable model adjusted for age and AML type this difference was not statistically significant. There was no significant association between LOF of mBCOR or mBCORL1 and ELN2017 risk groups regarding outcomes.

4. Discussion

We analyzed a large cohort of newly diagnosed and intensively treated AML patients according to their mutational status of BCOR and BCORL1. The respective proportions of mBCOR and mBCORL1 in the cohort were comparable to those reported in recent studies [6,21,26,27]. We found both mBCOR with LOF and mBCORL1 to be more prevalent in patients in the ELN2017 intermediate-risk group [4]. The proportion of sAML amongst patients harboring mBCOR was significantly higher than in their wild-type counterparts, confirming previous reports [37]. Mutations of BCORL1 were significantly more prevalent in females than in males, as has been previously suggested [27], and patients harboring mBCOR with LOF had significantly lower WBC, and a trend for lower peripheral blood blast counts. Mutations of BCOR frequently co-occurred with mutations of DNMT3A, RUNX1, TET2, NRAS, and BCORL1. Since both BCOR and DNMT3A function as epigenetic modifiers [7,38], a synergistic role in leukemogenesis has been reported recently [39,40]. In murine models, the co-occurrence of mBCOR and mutations of TET2, another epigenetic modifier [41] frequently associated with mBCOR in our cohort, has been reported to induce MDS [25,42]. This may further reinforce the notion that BCOR’s interaction with other DNA methylators plays a crucial role in leukemogenesis. RUNX1 and BCOR both play essential roles in the proliferation and differentiation of myeloid cells [10,43]. Therefore, the interplay of mBCOR and other essential regulators of normal myeloid development appears to be a factor that may promote leukemogenesis. Recent studies suggest, however, that perturbations of BCOR function alone do not suffice to induce malignant transformation [10,21,44], and thus co-mutations appear to be needed in order to drive leukemogenesis or, alternatively, sequential, secondary acquisition of LOF in BCOR following an oncogenic event, e.g., MDS driver mutation triggers disease progression towards AML, as suggested by the higher frequency of sAML among mBCOR patients. Accordingly, in our cohort of patients with mBCOR AML, the majority had at least two co-mutations, while only one patient harbored no other co-mutations targeted by our panel (Figure 1B). Similarly to mBCOR, co-mutations of mBCORL1 were RUNX1, DNMT3A, and TET2, as well as BCOR and FLT3-ITD, which were only rarely co-mutations of mBCOR. Again, the majority of patients harboring mBCORL1 had at least two other co-mutations, and only one patient had no other co-mutations revealed by our panel (Figure 2B). As for mBCOR, this suggests a potential interplay of impaired BCORL1 function with other dysfunctional mechanisms of DNA methylation, cell differentiation, and signal transduction in leukemogenesis.
Regarding outcomes, we found no statistically significant differences between patients with mBCOR and mBCORL1 regarding CR rate and ED30, compared to wild-type patients. Interestingly, while we observed lower median EFS, RFS, and OS for both mBCOR and mBCORL1 in general, only LOF mutations of mBCOR were associated with significantly reduced EFS and RFS and a trend of reduced OS in multivariable testing adjusted for age, AML type, and ELN2017 risk. In MDS, Abuhadra et al. [23] recently reported significantly reduced OS for patients with frameshift mutations of BCOR, while general mutation status did not affect OS. Previous studies of mBCOR in AML have reported poorer outcomes to often be associated with distinct co-mutations; however, a significant association of LOF mutations of BCOR as independent markers of poor outcomes has not yet been reported in AML. Terada et al. [27] reported reduced OS in AML patients who were younger than 65 years, wild-type FLT3, and had intermediate-risk cytogenetics. Grossmann et al. [26] reported a higher prevalence of mBCOR and a trend of reduced OS in normal-karyotype AML with no mutations in NPM1, FLT3-ITD, CEBPA, or MLL-PTD. However, in a validation cohort no association between mBCOR and OS was observed. Nevertheless, in both cohorts reduced EFS was detected [26]. Recently, Eisfeld et al. [45] reported reduced survival for patients in the ELN2017 favorable-risk group who harbored mutations in BCOR or SETBP1. Our findings underline the complexity of the mutational landscape of AML, where even mutational variants of rare mutations have to be considered in order to determine their clinical and prognostic effects. Future work needs to focus on the implementation of LOF mutations of BCOR in risk stratification tools for AML management.

5. Conclusions

In conclusion, both mBCOR and mBCORL1 are rare but recurrent mutations in AML. While previous studies suggested poor outcomes for mBCOR in AML, especially in the context of co-occurring mutations, we found loss-of-function mutations of mBCOR to be independent markers of poor outcomes in AML, while mBCORL1 was not significantly associated with outcomes in multivariable testing.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/cancers13092095/s1, Table S1: Summary of the 54 genes targeted by the TruSight Myeloid Sequencing Panel.

Author Contributions

Conceptualization, J.-N.E., S.S. (Sebastian Stasik), C.T. and J.M.M.; Data curation, M.K.; Formal analysis, J.-N.E., S.S. (Sebastian Stasik), M.K., C.R., A.K., S.S. (Sebastian Scholl), A.H., M.C., T.H.B., R.N. (Ralph Naumann), B.S., V.K., H.E., M.S., A.B., A.N., K.S.-E., C.S., S.W.K., R.H., M.H., N.F., R.N. (Richard Noppeney), U.K., C.D.B., M.K., Z.R., U.P., W.E.B., J.M., H.S., C.M.-T., G.E., F.S., F.K., J.S., M.B., C.T. and J.M.M.; Investigation, J.-N.E., S.S. (Sebastian Stasik), M.K., C.R., A.K., S.S. (Sebastian Scholl), A.H., M.C., T.H.B., R.N.(Ralph Naumann), B.S., V.K., H.E., M.S., A.B., A.N., K.S.E., C.S., S.W.K., R.H., M.H., N.F., R.N. (Richard Noppeney), U.K., C.D.B., M.K., Z.R., U.P., W.E.B., J.M., H.S., C.M.-T., G.E., F.S., F.K., J.S., M.B., C.T. and J.M.M.; Methodology, J.-N.E., S.S. (Sebastian Stasik), M.K., C.T. and J.M.M.; Project administration, M.B., C.T. and J.M.M.; Resources, J.-N.E., S.S. (Sebastian Stasik), M.K., C.R., A.K., S.S. (Sebastian Scholl), A.H., M.C., T.H.B., R.N. (Ralph Naumann), B.S., V.K., H.E., M.S., A.B., A.N., K.S.E., C.S., S.W.K., R.H., M.H., N.F., R.N. (Richard Noppeney), U.K., C.D.B., M.K., Z.R., U.P., W.E.B., J.M.., H.S., C.M.-T., G.E., F.S., F.K., J.S., M.B., C.T. and J.M.M.; Supervision, C.T. and J.M.M.; Validation, J.-N.E., S.S. (Sebastian Stasik), C.T. and J.M.M.; Visualization, J.-N.E. and S.S. (Sebastian Stasik); Writing—original draft, J.-N.E.; Writing—review & editing, S.S. (Sebastian Stasik), M.K., C.R., A.K., S.S. (Sebastian Scholl), A.H., M.C., T.H.B., R.N. (Ralph Naumann), B.S., V.K., H.E., M.S., A.B., A.N., K.S.E., C.S., S.W.K., R.H., M.H., N.F., R.N. (Richard Noppeney), U.K., C.D.B., M.K., Z.R., U.P., W.E.B., J.M., H.S., C.M.-T., G.E., F.S., F.K., J.S., M.B., C.T. and J.M.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The study was conducted in accordance with the guidelines of the Declaration of Helsinki. This study was carried out under the auspices of the German Study Alliance Leukemia (SAL). All mentioned studies were approved by the Institutional Review Board of the Dresden University of Technology (approval number: EK 98032010).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study. Written informed consent has been obtained from the patient(s) to publish this paper.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy issues.

Acknowledgments

This work was carried out under the auspices of the German SAL. We thank all involved patients, nurses, laboratory technicians, and physicians for their contributions. Open Access Funding by the Publication Fund of the TU Dresden.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Mutational spectrum of BCOR-mutated AML. (A) BCOR variant allele frequency (VAF) percentages. (B) Number of co-mutations in addition to mutated BCOR. (C) Clonal rank of BCOR mutations determined by comparing BCOR VAF to co-mutated somatic driver variants. (D) Mutated BCOR domain, undetermined (grey). (E) Effect of mutated BCOR, loss-of-function (transcriptional stop, green), or unknown (no transcriptional stop, grey). (F) Most common co-mutations. Mutations with a frequency of <10% not shown: ASXL1, GATA2, WT1, SF3B1, U2AF1, TP53, ZRSR2, IKZF1, KIT, ETV6, EZH2, FLT3-TKD, JAK2, PHF6, CBL, CBLB, CSF3R, NOTCH1, PDGFRA, SMC3.
Figure 1. Mutational spectrum of BCOR-mutated AML. (A) BCOR variant allele frequency (VAF) percentages. (B) Number of co-mutations in addition to mutated BCOR. (C) Clonal rank of BCOR mutations determined by comparing BCOR VAF to co-mutated somatic driver variants. (D) Mutated BCOR domain, undetermined (grey). (E) Effect of mutated BCOR, loss-of-function (transcriptional stop, green), or unknown (no transcriptional stop, grey). (F) Most common co-mutations. Mutations with a frequency of <10% not shown: ASXL1, GATA2, WT1, SF3B1, U2AF1, TP53, ZRSR2, IKZF1, KIT, ETV6, EZH2, FLT3-TKD, JAK2, PHF6, CBL, CBLB, CSF3R, NOTCH1, PDGFRA, SMC3.
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Cancers 13 02095 g002 550
Figure 2. Mutational spectrum of BCORL1-mutated AML. (A) BCORL1 variant allele frequency (VAF) percentages. (B) Number of co-mutations in addition to mutated BCORL1. (C) Clonal rank of BCORL1 mutations determined by comparing BCORL1 VAF to co-mutated somatic driver variants. (D) Effect of mutated BCORL1, loss-of-function (transcriptional stop, green), or unknown (no transcriptional stop, grey). (E) Most common co-mutations. Mutations with a frequency of <10% not shown: FLT3-TKD, IDH1, SRSF2, KIT, TP53, PTPN11, SF3B1, U2AF1, WT1, ZRSR2, STAG2, ETV6, EZH2, GATA2, KDM6A, ASXL1, CBL, KRAS, MYD88, SETBP1, JAK2, SMC3.
Figure 2. Mutational spectrum of BCORL1-mutated AML. (A) BCORL1 variant allele frequency (VAF) percentages. (B) Number of co-mutations in addition to mutated BCORL1. (C) Clonal rank of BCORL1 mutations determined by comparing BCORL1 VAF to co-mutated somatic driver variants. (D) Effect of mutated BCORL1, loss-of-function (transcriptional stop, green), or unknown (no transcriptional stop, grey). (E) Most common co-mutations. Mutations with a frequency of <10% not shown: FLT3-TKD, IDH1, SRSF2, KIT, TP53, PTPN11, SF3B1, U2AF1, WT1, ZRSR2, STAG2, ETV6, EZH2, GATA2, KDM6A, ASXL1, CBL, KRAS, MYD88, SETBP1, JAK2, SMC3.
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Cancers 13 02095 g003 550
Figure 3. Impact of BCOR mutations on outcomes. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with wild-type (orange) and mutated (turquoise) BCOR compared using the logrank test.
Figure 3. Impact of BCOR mutations on outcomes. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with wild-type (orange) and mutated (turquoise) BCOR compared using the logrank test.
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Cancers 13 02095 g004a 550Cancers 13 02095 g004b 550
Figure 4. Impact of BCOR loss-of-function mutations on outcomes. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with mutations of BCOR resulting in a transcriptional stop and loss-of-function (green), BCOR mutations without transcriptional stop resulting in unknown function (blue), and wild-type BCOR (orange) compared using the logrank test.
Figure 4. Impact of BCOR loss-of-function mutations on outcomes. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with mutations of BCOR resulting in a transcriptional stop and loss-of-function (green), BCOR mutations without transcriptional stop resulting in unknown function (blue), and wild-type BCOR (orange) compared using the logrank test.
Cancers 13 02095 g004aCancers 13 02095 g004b
Cancers 13 02095 g005 550
Figure 5. Impact of BCORL1 mutations on outcome. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with wild-type (orange) and mutated (turquoise) BCORL1 compared using the logrank test.
Figure 5. Impact of BCORL1 mutations on outcome. Kaplan–Meier plots for (A) event-free survival, (B) relapse-free survival, and (C) overall survival in AML, with wild-type (orange) and mutated (turquoise) BCORL1 compared using the logrank test.
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Table
Table 1. Patient characteristics for mBCOR.
Table 1. Patient characteristics for mBCOR.
ParameterwtBCORmBCOR-LOFmBCOR–UFp-Value
N. of patients14584823
Age, median (IQR)55 (44–64)53 (45–65)61 (51–69)0.165
Sex, n (%) 0.150
Female689 (47.3)26 (54.2)15 (65.2)
Male769 (52.7)22 (45.8)8 (34.8)
AML type, n (%) 0.065
De novo1235 (85.6)37 (78.7)15 (68.2)
sAML158 (11)9 (19.1)6 (27.3)
tMN49 (3.4)1 (2.1)1 (4.5)
ELN2017, n (%) <0.001
Favorable561 (41.7)1 (2.3)8 (38.1)
Intermediate522 (38.8)38 (86.4)6 (28.6)
Adverse261 (19.4)5 (11.4)7 (33.3)
Complex karyotype, n (%) 0.111
No1178 (87.8)44 (93.6)19 (82.6)
Yes164 (12.2)3 (6.4)4 (17.4)
FLT3-ITD, n (%) 0.022
wt1114 (76.9)44 (93.6)19 (82.6)
m334 (23.1)3 (6.4)4 (17.4)
NPM1, n (%) <0.001
wt961 (67)45 (97.8)16 (69.6)
m474 (33)1 (2.2)7 (30.4)
WBC, median (IQR) in GPt/L20.85 (4.96–56.4)4 (1.82–16.88)16.5 (6.46–34.65)<0.001
Hb, median (IQR) in mmol/L5.9 (5.0–7.0)6.15 (5.25–7.78)5.59 (5.06–6.01)0.211
Platelets, median (IQR) in GPt/L50 (27.0–92.0)54 (25.5–106)73 (41.5–118.5)0.176
LDH, median (IQR) in mmol/L457 (290.25–795.0)306 (223.65–458.3)407 (254.5–897)0.001
BM blasts (%), median (IQR)63 (44–79)58.75 (48.88–72.12)61 (45–72.73)0.874
PB blasts (%), median (IQR)42 (13–75)19 (6–59)39 (19.75–70)0.076
wt: wild-type; m-: mutated; LOF: loss-of-function effect (transcriptional stop); UF: unknown effect (no transcriptional stop); sAML: secondary acute myeloid leukemia; tMN: therapy-related acute myeloid leukemia; ELN2017: European Leukemia Net 2017; WBC: white blood cells; BM: bone marrow; PB: peripheral blood; n/N: number; IQR: interquartile range.
Table
Table 2. Patient characteristics for mBCORL1.
Table 2. Patient characteristics for mBCORL1.
ParameterwtBCORL1mBCORL1p-Value
N. of patients147653
Age, median (IQR)55 (44–65)52 (43–62)0.177
Sex, n (%) 0.044
Female697 (47.2)33 (62.3)
Male779 (52.8)20 (37.7)
AML type, n (%) 0.621
De novo1244 (85.3)43 (82.7)
sAML167 (11.4)6 (11.5)
tMN48 (3.3)3 (5.8)
ELN2017, n (%) <0.001
Favorable564 (41.5)6 (12)
Intermediate531 (39.1)35 (70)
Adverse264 (19.4)9 (18)
Complex karyotype, n (%) 0.975
No1192 (88)39 (86.7)
yes163 (12)6 (13.3)
FLT3-ITD, n (%) 0.782
wt1138 (77.6)39 (75)
m328 (22.4)13 (25)
NPM1, n (%) 0.006
wt977 (67.3)45 (86.5)
m475 (32.7)7 (13.5)
WBC, median (IQR) in GPt/L20.09 (4.8–55.27)14.35 (3.55–54.95)0.340
Hb, median (IQR) in mmol/L5.9 (5.0–7.0)6.2 (5.3–7.0)0.341
Platelets, median (IQR) in GPt/L51 (27.0–93.3)50.5 (30.0–90.5)0.727
LDH, median (IQR) in mmol/L452 (288.0–794.8)333 (254.0–621.0)0.053
BM blasts (%), median (IQR)63 (44–79)65.25 (48.88–82.75)0.299
PB blasts (%), median (IQR)40.5 (12–74)48.5 (15–78)0.419
wt: wild-type; m-: mutated; sAML: secondary acute myeloid leukemia; tMN: therapy-related acute myeloid leukemia; ELN2017: European Leukemia Net 2017; WBC: white blood cells; BM: bone marrow; PB: peripheral blood; n/N: number; IQR: interquartile range.
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MDPI and ACS Style

Eckardt, J.-N.; Stasik, S.; Kramer, M.; Röllig, C.; Krämer, A.; Scholl, S.; Hochhaus, A.; Crysandt, M.; Brümmendorf, T.H.; Naumann, R.; et al. Loss-of-Function Mutations of BCOR Are an Independent Marker of Adverse Outcomes in Intensively Treated Patients with Acute Myeloid Leukemia. Cancers 2021, 13, 2095. https://doi.org/10.3390/cancers13092095

AMA Style

Eckardt J-N, Stasik S, Kramer M, Röllig C, Krämer A, Scholl S, Hochhaus A, Crysandt M, Brümmendorf TH, Naumann R, et al. Loss-of-Function Mutations of BCOR Are an Independent Marker of Adverse Outcomes in Intensively Treated Patients with Acute Myeloid Leukemia. Cancers. 2021; 13(9):2095. https://doi.org/10.3390/cancers13092095

Chicago/Turabian Style

Eckardt, Jan-Niklas, Sebastian Stasik, Michael Kramer, Christoph Röllig, Alwin Krämer, Sebastian Scholl, Andreas Hochhaus, Martina Crysandt, Tim H. Brümmendorf, Ralph Naumann, and et al. 2021. "Loss-of-Function Mutations of BCOR Are an Independent Marker of Adverse Outcomes in Intensively Treated Patients with Acute Myeloid Leukemia" Cancers 13, no. 9: 2095. https://doi.org/10.3390/cancers13092095

APA Style

Eckardt, J. -N., Stasik, S., Kramer, M., Röllig, C., Krämer, A., Scholl, S., Hochhaus, A., Crysandt, M., Brümmendorf, T. H., Naumann, R., Steffen, B., Kunzmann, V., Einsele, H., Schaich, M., Burchert, A., Neubauer, A., Schäfer-Eckart, K., Schliemann, C., Krause, S. W., ... Middeke, J. M. (2021). Loss-of-Function Mutations of BCOR Are an Independent Marker of Adverse Outcomes in Intensively Treated Patients with Acute Myeloid Leukemia. Cancers, 13(9), 2095. https://doi.org/10.3390/cancers13092095

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