High-Level Production of Succinic Acid from Crude Glycerol by a Wild Type Organism
Abstract
:1. Introduction
2. Results
2.1. Reference Cultivation with Pure Glycerol
2.2. Cultivation with Crude Glycerol
2.2.1. Cultivation with Crude Glycerol in 100 mL Laboratory Glass Bottles
2.2.2. Cultivation with Crude Glycerol in the 350 mL Bioreactor System Compared to Pure Glycerol
2.3. Crystallization and Purity Determination
3. Discussion
4. Materials and Methods
4.1. Microorganism
4.2. Media composition and Chemicals
4.3. Cultivation
4.4. Analytical Methods
4.5. Crystallization and Purity Determination
Author Contributions
Funding
Conflicts of Interest
References
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Reference Cultivation in 100 mL Laboratory Glass Bottle | ||
---|---|---|
Initial 50 g L−1 Substrate | Initial 85 g L−1 Substrate | |
CEnd, SA / g L−1 | 49.4 | 88.8 |
CEnd, LA / g L−1 | 3.2 | 6.3 |
Pmax / g L−1 h−1 | 0.76 | 0.31 |
P / g L−1 h−1 | 0.37 | 0.22 |
Y1 / g g−1 | 0.9 | 0.94 |
Pure Glycerol | Crude Glycerol | |
---|---|---|
CEnd, SA / g L−1 | 49.4 | 46.8 |
CEnd, LA / g L−1 | 3.2 | 2.3 |
Pmax / g L−1 h−1 | 0.76 | 0.62 |
P / g L−1 h−1 | 0.37 | 0.35 |
Y1 / g g−1 | 0.9 | 0.88 |
Pure Glycerol | Crude Glycerol | |
---|---|---|
CEnd, SA / g L−1 | 117 | 86.9 |
CEnd, LA / g L−1 | 8.6 | 6 |
Pmax / g L−1 h−1 | 0.69 | 0.55 |
P / g L−1 h−1 | 0.34 | 0.33 |
Y1 / g g−1 | 1.3 | 0.9 |
Microorganism | Substrate | Succinic Acid | Yield | Productivity | Reference |
---|---|---|---|---|---|
(g L−1) | (g g−1) | (g L−1h−1) | |||
Actinobacillus succinogenes 130Z | Glucose | 106 | 0.8 | 1.34 | [32] |
Xylose1 | 29.4 | 0.68 | 3.41 | [33] | |
Basfia succiniciproducens DD1 | Glucose | 31.7 | 0.63 | 1.32 | [34] |
Basfia succiniciproducens DD1 | Crude glycerol | 8.4 | 1.2 | 0.9 | [35] |
Actinobacillus succinogenes ATCC 55618 | Glycerol | 29.3 | 1.23 | 0.27 | [36] |
Actinobacillus succinogenes GXAS 137 | Sugar cane molasses | 64.7 | 0.86 | 1.4 | [37] |
Isolate AKR177 | Glycerol | 117 | 1.3 | 0.34 | This study |
Isolate AKR177 | Crude glycerol | 86.9 | 0.9 | 0.33 | This study |
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Kuenz, A.; Hoffmann, L.; Goy, K.; Bromann, S.; Prüße, U. High-Level Production of Succinic Acid from Crude Glycerol by a Wild Type Organism. Catalysts 2020, 10, 470. https://doi.org/10.3390/catal10050470
Kuenz A, Hoffmann L, Goy K, Bromann S, Prüße U. High-Level Production of Succinic Acid from Crude Glycerol by a Wild Type Organism. Catalysts. 2020; 10(5):470. https://doi.org/10.3390/catal10050470
Chicago/Turabian StyleKuenz, Anja, Lisa Hoffmann, Katharina Goy, Sarah Bromann, and Ulf Prüße. 2020. "High-Level Production of Succinic Acid from Crude Glycerol by a Wild Type Organism" Catalysts 10, no. 5: 470. https://doi.org/10.3390/catal10050470
APA StyleKuenz, A., Hoffmann, L., Goy, K., Bromann, S., & Prüße, U. (2020). High-Level Production of Succinic Acid from Crude Glycerol by a Wild Type Organism. Catalysts, 10(5), 470. https://doi.org/10.3390/catal10050470