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Article

Biochemical Response of Oakleaf Lettuce Seedlings to Different Concentrations of Some Metal(oid) Oxide Nanoparticles

by
Rita Jurkow
1,*,
Agnieszka Sękara
1,
Robert Pokluda
2,
Sylwester Smoleń
3 and
Andrzej Kalisz
1
1
Department of Horticulture, University of Agriculture in Krakow, 31-425 Kraków, Poland
2
Department of Vegetable Sciences and Floriculture, Mendel University in Brno, 691 44 Lednice, Czech Republic
3
Department of Plant Biology and Biotechnology, University of Agriculture in Krakow, 31-425 Kraków, Poland
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(7), 997; https://doi.org/10.3390/agronomy10070997
Submission received: 5 June 2020 / Revised: 8 July 2020 / Accepted: 9 July 2020 / Published: 11 July 2020
(This article belongs to the Special Issue From Biofortification to Tailored Crops and Food Products)
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Versions Notes

Abstract

:
Nanoparticles (NPs) significantly modify the physiological functions and metabolome of plants. The purpose of the study was to investigate the effect of CeO2, Fe2O3, SnO2, TiO2, and SiO2 nanoparticles, applied in foliar spraying of oakleaf lettuce at concentrations 0.75% to 6%, on the antioxidant enzyme activity and content of non-enzymatic antioxidants, chlorophyll pigments, fresh weight (FW) and dry weight (DW). It was found that 3% Fe2O3-NPs caused a 27% decrease in fresh weight compared to control plants. Fe2O3-NPs caused an increase in dry weight (g 100 g−1 FW) when compared to the control for all concentrations, but total DW (g per plant) was similar for all NPs treatments. Significant increases in chlorophyll a + b content after treatment with 1.5% and 6% SiO2-NPs, 3% Fe2O3-NPs, and 3% TiO2-NPs were noted. Fe2O3-NPs caused a significant increase in the activity of ascorbate peroxidase, guaiacol peroxidase, and catalase (only for 3% Fe2O3-NPs). SnO2-NPs decreased ascorbate peroxidase (APX) and guaiacol peroxidase (GPOX) activity (for all tested concentrations) but increased catalase (CAT) activity when a 3% suspension of these NPs was applied. The level of glutathione (GSH) increased due to application of all metal/metalloid oxides, with the exception of SnO2-NPs. When all concentrations of TiO2-NPs were applied, L-ascorbic acid increased significantly, as well as increasing at higher concentrations of SiO2-NPs (3% and 6%) and at 0.75% and 3% Fe2O3-NPs. SiO2-NPs and TiO2-NPs significantly elevated the carotenoid and total phenolic content in treated plants compared to the control. The total antioxidant capacity of plants treated with 3% CeO2-NPs was almost twice as high as that of the control.

1. Introduction

Nanoparticles (NPs) are atomic or molecular aggregates with at least one dimension in the range from 1 to 100 nm [1]. Metal or metalloid-based NPs (nanometals or nanoscale metal/metalloid oxides) belong to the engineered type of NPs and not only have unique chemical and physical properties in comparison to their bulk counterparts but also different biological actions [2]. Because of the widespread use of nanomaterials in industry and in consumer products, it is expected that NPs can be transferred and accumulated in aquatic, terrestrial, and atmospheric environments [3], where their fate and behavior seems to be unknown. The reactiveness of metal-based NPs in plants is determined by their metal compound, size, high surface-to-volume ratio, shape, and, most importantly, the dose at which they are effective [4]. After entering plant cells, NPs might directly provoke either alterations of membranes and other cell structures and molecules, or activity of protective mechanisms [5]. The indirect effects of NPs are caused inter alia by the release of toxic ions (e.g., metal ions), enhancement of the bioavailability of some toxic compounds, or by causing overproduction of reactive oxygen species (ROS) [1,2,6].
It has been reported that the impact of NPs on plants can be diverse and depends on the type of NPs, their physicochemical properties, concentration, exposure time, and plant species [7]. Most studies have demonstrated that an excess of metal-based NPs can cause negative effects like reduced germination, dry weight, biomass, and transpiration, disturbances in the photosynthetic process, chlorophyll degradation, protein reduction, DNA damage, nutrient displacement, and others [8,9]. NPs trigger an oxidative burst by interfering with the electron transport chain and the production of ROS [1]. So it may be expected that the antioxidant defense mechanisms of plants based on ascorbate, glutathione, α-tocopherol, or several enzymatic scavengers of ROS such as superoxide dismutase, peroxidases, and catalase, might provide protection against such adverse effects of NPs [5,10]. Besides the toxicity of NPs, they may also have some positive effects on plants. For example, TiO2-NPs have been found to induce spinach seed germination; moreover, during growth, the plant dry weight is increased, as are chlorophyll synthesis, ribulosebisphosphate carboxylase/oxygenase activity, and photosynthetic rate [11]. Positive or inconsequential effects of non-organic NPs on food crops have been already presented [6,8], but there is no full explanation of the mechanisms of NPs action. Taken together, the literature presents differentiated data on the impact of particular NPs on plants due to different sizes, concentrations, application routes etc., so the influence of NPs on plants still requires further investigation and standardizing research procedures.
Interactions between NPs and plants are a problem that we have to face in relation to rapidly developing nanotechnology. In our study we focused on the exposure of plant leaves to different metal oxide and metalloid oxide nanoparticles (MO-NPs) with various physicochemical properties, applied at different concentrations. We chose five commercial nanoparticles of metal oxides/metalloid oxide, including four nanoparticles (CeO2, Fe2O3, TiO2, and SiO2) for which there are certain data on their impact (beneficial or negative) on plants and one nanoparticle (SnO2) with an indistinct role and impact on plants. Our assumption was that NPs treatment will cause abiotic stress followed by activation of antioxidative mechanisms. Moreover, the operation of these mechanisms and the intensity of the stress will be different depending on the type of MO-NPs applied and the concentration used.

2. Materials and Methods

2.1. Characteristics of Nanoparticles

The MO-NPs utilized in this study are commercially available from PlasmaChem GmbH (Berlin, Germany) as aqueous suspensions. Nanoparticles of cerium oxide (CeO2-NPs), iron (III) oxide (α-Fe2O3-NPs), silicon dioxide (SiO2-NPs), tin(IV) oxide (SnO2-NPs), and titanium(IV) dioxide (TiO2-NPs) were used in the experiment. The basic characteristics of NPs are: CeO2-NPs—average particle size: 4 ± 2 nm, specific surface area: ca. 20 m2 g−1; Fe2O3-NPs—hematite phase, average particle size: 6 ± 2 nm; SiO2-NPs—average particle size: ca. 10 nm, specific surface area: ca. 320 m2 g−1; SnO2-NPs—average particle size: 6 ± 2 nm; TiO2-NPs—anatase phase, average particle size: 6 ± 2 nm, specific surface area: ca. 140 m2 g−1. All MO-NPs were delivered as 5 wt% aqueous colloidal suspensions with the exception of SiO2-NPs (30 wt%). In this study, several concentrations of MO-NP suspensions were prepared (see next section) by adding deionized water to the stock suspension.

2.2. Plant Material and Nanoparticle Application

Seedlings of oakleaf lettuce (Lactuca sativa L. var. foliosa Bremer) cv. Kiribati (seeds from Rijk Zwaan Polska Sp. z o.o., Warszawa, Poland) were purchased from the Krasoń vegetable seedling producers group (Piaski, Poland). We chose this plant because of its fairly large importance as a salad crop, its rapid growth and delicate leaves of large leaf surface, without wax coating, making it a good model for testing effects of foliar exposure to nanoparticles. Lettuce is a plant often taken into account in studying response to different stresses, including studies of metabolism alterations caused by nanoparticles [12,13]. Production of oakleaf lettuce seedlings takes around 3 weeks from sowing to planting out.
Seeds were sown into cubic peat pots (dimensions 4 × 4 × 4 cm, total volume of 64 cm3) placed in plastic boxes (150 pots per box in total). During cultivation, 0.3% solution of Goëmar Goteo (Arysta LifeScience Polska Sp. z o.o., Warsaw, Poland) was applied once as a plant biostimulator. Goëmar Goteo contains 13% P2O5, 5% K2O and Ascophyllum nodosum filtrate. Two-week-old seedlings (4–5 leaves) were placed on a table in a greenhouse and the plants were irrigated by flooding the table. If necessary, flooded irrigation was used during the experiment. After 2 more days, the following concentrations of NPs were applied to the leaf surface, using a hand sprayer with a mist spray nozzle: 6%, 3%, and 1.5% of TiO2 and SiO2; 3% and 1.5% of CeO2, SnO2, and Fe2O3; 0.75% Fe2O3 was also used. Based on preliminary tests (the seedlings having 3–4 leaves were sprayed with different concentrations of nanoparticles), the highest applied NPs concentrations that did not cause severe leaf necroses and plant deformation (visual estimation) were selected for further experiments. Next, 1–2 lower concentrations were prepared and used to determine whether also nanoparticles in a more diluted suspension will affect metabolic processes in plants. For each treatment, there were three plastic seedling boxes with 150 plants per box (450 plants per treatment); particular suspensions were applied evenly on the leaves in a dose of 50 cm3 per box (ca. 0.33 cm3 per plant). Control plants were sprayed at the same time with deionized water. No additional fertilization was used during trial. Plant samples for laboratory analyses were taken after 7 days. Leaves were washed with tap water and rinsed with deionized water. Then all leaves from a treatment were mixed, fresh laboratory samples were taken from these leaves to determine dry weight, L-ascorbic acid and glutathione. The rest of the collected leaves were inserted into an ultra-deep freezer to a temperature of −40°C for further chemical analyses.

2.3. Fresh and Dry Weight

The above-ground plants were weighed with a Sartorius A120S balance (Sartorius AG, Göttingen, Germany) to determine fresh weight (FW) and oven-dried at 65 °C until constant weight to determine dry weight (DW). Then, the difference in weight was calculated and expressed as the FW percentage. DW content was also re-calculated and presented in grams per plant shoot.

2.4. Chlorophyll and Carotenoid Content

The chlorophyll (a and b) and carotenoid content was determined 7 days after NP treatment according to the procedure of Lichtenthaler and Wellburn [14]. Leaf samples (0.1 g) were ground with the addition of 3 mg of magnesium carbonate (MgCO3) as a pigment stabilizer, and chlorophyll (Chl a and Chl b) and carotenoids (Car) were extracted in 80% (v/v) aqueous acetone (25 cm3). After 0.5 h incubation in the dark, the suspension obtained was filtered through a filter paper (POCH SA, No. 978774513, Gliwice, Poland). Absorbance was determined at wavelengths of 646, 663, and 470 nm using a spectrophotometer (UV-VIS Helios Beta, Thermo Fisher Scientific Inc., Waltham, USA). Absorption measurements were used to quantify the chlorophyll a, chlorophyll b, and total carotenoid content, based on the equations reported by Lichtenthaler and Wellburn [14]. Additionally, the total chlorophyll (a + b) content and the ratios of chlorophyll a to chlorophyll b (Chl a:Chl b) and of carotenoids to total chlorophylls (Car:Chls) were also calculated.

2.5. Antioxidant Enzyme Assays

Catalase (CAT, EC 1.11.1.6) activity was determined according to Aebi [15]. Leaves (5 g) were ground in an ice bath (4 °C) with 10 cm3 of extraction buffer (0.05 M potassium phosphate buffer). The extract was centrifuged for 15 min at 3492 g and 4 °C. Test tubes contained 1.8 cm3 of 0.05 M phosphate buffer (pH 7.0) and 1.0 cm3 of 0.05% H2O2 solution in 0.05 M potassium phosphate buffer (pH 7.0). The supernatant (0.2 cm3) was used for enzymatic assays. CAT activity was estimated by the decrease in absorbance of H2O2 against a blank at wavelength of 240 nm (UV-VIS Helios Beta spectrophotometer) over a period of 5 min and expressed in µmol of H2O2 min−1 g−1 FW.
The procedure for determining ascorbate peroxidase and guaiacol peroxidase activity started with preparation of a mixture at 4 °C containing 4 g of leaf samples homogenized in 10 cm3 of 50 mM potassium phosphate buffer (pH 7.0) with 1 mM ethylenediaminetetraacetic acid (EDTA), 1% soluble polyvinyl pyrrolidone (PVP), and 1 mM phenylmethylsulfonyl fluoride (PMSF). The mixture was centrifuged at 13,968 g for 15 min at 4 °C, and the supernatant (0.4 cm3 for guaiacol peroxidase and 0.5 cm3 for ascorbate peroxidase assay) was used for enzyme activity assays. The activity of ascorbate peroxidase (APX, EC 1.11.1.11) was measured using the method of Nakano and Asada [16]. The reaction mixture consisted of 50 mM potassium phosphate buffer (pH 7.0) containing 0.5 mM ascorbate, 0.1 mM H2O2, and 0.15 cm3 of the enzyme extract. Oxidation of ascorbate detected as a decrease in absorbance at 290 nm (UV-VIS Helios Beta spectrophotometer) was followed 5 min after starting the reaction. The difference in absorbance was divided by the ascorbate molar extinction coefficient (ε = 2.8 mM−1 cm−1). The activity of the enzyme was expressed as µg ascorbate (AsA) min−1 g−1 FW. The activity of guaiacol peroxidase (GPOX, EC 1.11.1.7) was assayed according to Zhang et al. [17], based on the oxidation of guaiacol using hydrogen peroxide. The reaction mixture consisted of 50 mM phosphorus buffer (pH 7.0), hydrogen peroxide (1%), and guaiacol (4%). The reaction was started by adding 0.4 cm3 of enzyme extract to the reaction mixture at 25 °C; GPOX activity was measured by the increase in absorbance at 470 nm due to guaiacol oxidation using a UV-VIS Helios Beta spectrophotometer over a period of 3 min (ε = 26.6 mM−1 cm−1). The activity level was expressed as µmol tetraguaiacol min−1 g−1 FW.

2.6. Glutathione Content

The reduced form of glutathione (GSH) was determined according to the method described by Guri [18] with modifications. Fresh leaf tissue (2.5 g) was homogenized in an ice bath (4 °C) with the addition of 6 cm3 of 0.5 mM EDTA and 3% trichloroacetic acid (TCA) and the homogenate was centrifuged at 4 °C for 10 min at 6208 g. In order to bring the pH of the solution to ca. 7.0, K-phosphate buffer was used. The content of reduced GSH was assessed using Ellman’s reagent (5,5-dithiobis-2-nitrobenzoic acid, DTNB) on a UV-VIS Helios Beta spectrophotometer. The solution extinction was measured at the wavelength λ = 412 nm. The absorbance of a mixture of 2.0 cm3 of plant homogenate and 1.0 cm3 of 0.2 M K-phosphate buffer, which absorbed part of the radiation, was measured as a blind sample. The concentration of GSH was calculated from the standard curve and expressed as µg g−1 FW.

2.7. L-ascorbic Acid Content

The content of L-ascorbic acid was determined by Tillman’s titration method as described by Krełowska-Kułas [19]. Fresh plant leaves (12.5 g) were homogenized with 50 cm3 acetic acid applied as an acidity regulator. After 30 min, the mixture was titrated with Tillman’s reagent (2,6-dichlorophenol-indophenol). Excessive dye in an acidic environment gives a pink color and marks the end point of the titration. Then, the content of L-ascorbic acid in the sample was calculated based on the amount of the changed solution of 2,6-dichlorophenol-indophenol used for titration.

2.8. Total Phenolic Content

The concentration of total phenols in plant extracts was estimated by the Folin–Ciocalteu colorimetric procedure described by Djeridane et al. [20] with modifications. Two grams of plant material was mixed with 10 cm3 of 80% methanol and centrifuged for 10 min at 3492 g. Next, plant extracts (0.1 cm3) were mixed with 2 cm3 of 2% sodium carbonate (Na2CO3); after 2 min, 0.1 cm3 of Folin–Ciocalteu reagent, mixed with deionized water (1:1 v/v), was added to the test tubes. The final mixture was shaken and then incubated for 45 min in the dark at room temperature before measuring the absorbance at 750 nm using a UV-VIS Helios Beta spectrophotometer against a reference solution. The results were determined from a standard gallic acid curve and expressed as milligrams of gallic acid equivalents (GAE) per gram FW (mg GAE g−1 FW).

2.9. DPPH• Radical Scavenging Activity

The antioxidant activity (AA) was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) as a free radical [21]. The decrease in absorbance was measured at 517 nm with a UV-VIS Helios Beta spectrophotometer. Ground plant material (2.5 g) in 80% methanol was centrifuged (3492 g, 10 min at 4 °C). The test tubes contained 0.1 mL of supernatant and 4.9 mL of 0.1 mM DPPH• dissolved with 80% methanol. The reaction mixture was shaken and incubated in the dark at 20 °C for 15 min. The following formula was used to calculate DPPH• radical scavenging activity: AA [%] = [(A0 − A1)/A0] × 100; AA—antioxidant activity, A0—absorbance of the reference solution, A1—absorbance of the test solution. AA was expressed as the percentage of DPPH• free radical scavenging.

2.10. Content of Ce, Fe, Si, Sn, and Ti

Plant material (finely chopped lettuce leaves, sampled as described earlier) was oven-dried at 65 °C, to a constant weight, and ground to a fine powder using a Pulverisette 14 ball mill (Fritsch GmbH, Idar-Oberstein, Germany) with a 0.5 mm sieve. The total content of the elements Ce, Fe, Si, Sn, and Ti was analyzed in ground samples of lettuce leaves. Before determination of the elements by spectrometric technique, microwave mineralization of the samples was executed. A separate two-step digestion was used for mineralization of the samples for the determination of Si than for the digestion of samples for the analysis of the total content of Ce, Fe, Sn, and Ti. A Mars 5 Xpress microwave digestion system (CEM Corporation, Matthews, NC, USA) and 100 cm3 TFM vessels were used.
The Si analysis was performed using a Prodigy ICP-OES spectrometer (Teledyne Leeman Labs, Hudson, NH, USA). It was preceded by two-stage microwave mineralization of samples according to the method of Barros et al. [22]. In the first stage, 100 mg lettuce samples were placed in TFM vessels and mineralized in 5 mL of 1 M super-pure HNO3 (Merck no. 100443.2500) plus 5 mL of H2O2 (30% v/v) in a microwave digestion system. The following mineralization procedure was applied: 5 min to achieve a temperature of 120 °C which was maintained for 5 min; 5 min to reach 160 °C which was maintained for 5 min; 3 min to reach 230 °C which was maintained for 5 min. The second stage was after cooling, when the TFM vessels were open and 5 mL of 1.5 M NaOH was added to the mineralized sample in the TFM vessel. The following program was used for mineralization in the second stage of digestion: 5 min to reach 150 °C which was maintained for 5 min; 5 min to reach 230 °C which was maintained for 10 min. After cooling, the samples were quantitatively transferred to 50 mL graduated flasks with 14 M HNO3.
In order to determine the content of Ce, Fe, Sn, and Ti, plant samples (3 g) were placed into 100 cm3 TFM vessels and mineralized in 10 cm3 of 65% super-pure HNO3 (Merck no. 100443.2500) in a Mars 5 Xpress microwave digestion system according to the method described in a previous publication [23]. After cooling, the samples were quantitatively transferred to 25 cm3 graduated flasks with redistilled water. These four elements were analyzed using an iCAP TQ ICP-MS/MS triple quadrupole spectrometer (ThermoFisher Scientific, Bremen, Germany). Determination was carried out using the following measurement modes for individual isotopes of elements: S-SQ-KED for 56Fe, 118Sn, and 49Ti and S-TQ-O2 for 140Ce, and 16O.

2.11. Statistical Analysis

The data obtained from this study were analyzed using Statistica 13.3 (TIBCO Software Inc., Palo Alto, CA, USA). Each nanoparticle was treated as a separate part of the experiment and were not statistically compared to each other, only to control. Differences between particular treatments (specific nanoparticle and its concentrations) and untreated control were analyzed using one-way ANOVA and Fisher post-hoc test. A p-value of less or equal than 0.05 was considered to be statistically significant. Means for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05, comparisons were performed using the Fisher test. Data for each measurement represent the mean of three biological replicates ± standard deviation (SD). Based on the activity of antioxidant enzymes and the content of non-enzymatic antioxidants, principal component analysis (PCA) was performed using Statistica 13.3 software (data were standardized before starting the analysis procedure) and the first two components (PC1 and PC2) explaining the maximum variance in the datasets were used to make biplots.

3. Results

A comparison was made on fresh and dry weights (per plants) and dry weight (g 100 g−1 FW) in response to nanoparticle treatments. Treatment of oakleaf lettuce seedlings with MO-NPs at different concentrations usually did not have a significant effect on the fresh weight (FW) of the plants in comparison to the control (Table 1). Only plants sprayed with 3% Fe2O3-NPs responded with a 27.1% decrease in FW compared to control plants. Dry weight (DW) (g 100 g−1 FW) increased when compared to the control for all tested concentrations of Fe2O3-NPs, indicating higher plant dehydration, especially after treatment with 3% suspension (in that case, the difference amounted to 1.220 g 100 g−1 FW). On the other hand, total DW (g per plant) was similar for all NP treatments and control, which pointed to no disturbances in anabolic processes efficiency.
There were significant increases in chlorophyll a content after treatment with the following NPs: 3% Fe2O3-NPs, 3% TiO2-NPs, and SiO2-NPs (all concentrations tested: 1.5%, 3%, 6%). The differences between these treatments and the control were 0.063, 0.154, 0.161, 0.065, and 0.161 mg g−1 FW, respectively. SiO2-NPs applied in a concentration of 1.5% and 6% increased the chlorophyll a content more than with a concentration of 3% of these nanoparticles. Applying SiO2-NPs to plant leaves at 1.5% and 6% caused significant increase in chlorophyll b concentration (by 0.027 and 0.026, respectively) compared to the control. Fe2O3-NPs used in a concentration of 3% increase chlorophyll b more than using a suspension of 0.75%, Compared to control, changes in chlorophyll b content of lettuce sprayed with Fe2O3-NPs were, however, negligible. As a result of foliar spraying with 3% TiO2-NPs, 3% Fe2O3-NPs, and SiO2-NPs at concentrations of 1.5% and 6%, total chlorophyll content significantly increased from 0.078 mg g−1 FW (3% Fe2O3-NPs) to 0.188 mg g−1 FW (1.5% SiO2-NPs) in comparison to the control. The highest chlorophyll a:chlorophyll b ratio (Chl a:Chl b) as well as carotenoids to chlorophylls ratio (Car:Chls) was an effect of the treatment with all concentrations of SiO2-NPs and TiO2-NPs (for Chl a:Chl b ratio not for 1.5% TiO2-NPs).
A significant increase in ascorbate peroxidase (APX) activity was observed at all concentrations of Fe2O3-NPs (Figure 1A). The greatest difference in APX activity was observed between control plants and oakleaf lettuce treated with 3% Fe2O3-NPs, reaching a level of 158.1%. Foliar application of CeO2-NPs in the form of a 1.5% suspension decreased APX activity by 32.3%. Plants treated with 1.5% and 3% SnO2 showed a decrease in APX activity (by 25.8% and 40.3%, respectively). TiO2-NPs applied at 1.5% and 3% also decreased APX activity by 25.8% and 29.0%, respectively. SiO2-NPs used in a concentration of 1.5% increased the activity APX more than with a suspension having a concentration of 3%. Similar to that of APX, guaiacol peroxidase (GPOX) activity also increased in lettuce as a result of foliar spraying with all tested concentrations of Fe2O3-NPs (Figure 1B). Plants treated with 3% Fe2O3-NPs had 246.0% higher GPOX enzyme activity than control plants. Moreover, GPOX activity gradually increased with an increasing concentration of Fe2O3-NPs. Additionally, spraying plants with 6% SiO2-NPs and 6% TiO2-NPs caused a 74.2% and 25.3%, respectively, increase in GPOX activity, in comparison to the control. Treatment with all tested concentrations of SnO2-NPs (1.5% and 3%) significantly decreased the activity of this enzyme, by 61.1% and 38.4% when compared to control. The highest catalase (CAT) activity occurred in plants treated with Fe2O3-NPs (Figure 1C), when a 3% suspension was used (the difference reached 387.5% in comparison to the control). Additionally, 3% SnO2 caused significant increase (by 100.0%) in CAT activity compared to control.
The level of glutathione (GSH) increased after application of almost all tested MO-NPs (Figure 2A). The only exceptions were plants treated with SnO2-NPs and 1.5% Fe2O3-NPs, where the GSH concentration was similar to that of the control. The greatest increase in GSH content, 91.1%, was observed when foliar spraying with 6% TiO2-NPs was performed. The smallest increase in GSH (by 30.9%), but significant in comparison to the control, was observed for treatment with 1.5% CeO2. The most pronounced effect of NPs on the L-ascorbic acid (LAA) content was shown for 0.75% and 3% Fe2O3-NPs concentrations (Figure 2B), the increase in LAA reached 51.4% and 54.9%, respectively, in comparison to control plants. All tested concentrations of TiO2-NPs also increased the LAA content, by 16.7–26.4%. Higher concentrations of SiO2-NPs (3% and 6%) also stimulated LAA biosynthesis in oakleaf lettuce seedlings. All concentrations of SiO2-NPs and TiO2-NPs caused a strong increase in the carotenoid (Car) content in treated plants compared to the control (Figure 2C). This increase in Car was the highest in the case of 1.5% SiO2-NPs (by 104.4%). Spraying plants with 3% Fe2O3-NPs also increased carotenoid content by 25.7%. CeO2-NPs, 0.75% and 1.5% Fe2O3-NPs, and SnO2-NPs did not affect the Car concentration in the plants.
The application of SiO2-NPs and TiO2-NPs caused an increase in the total phenolic (TP) concentration in oakleaf lettuce plants (Figure 3A). The differences compared to the control were in the range from 24.1% to 49.3% for 1.5% and 6% suspensions of TiO2-NPs, respectively, and from 34.8% to 47.7% for 1.5% and 6% suspensions of SiO2-NPs, respectively. Additionally, the highest concentrations (3%) of CeO2-NPs and Fe2O3-NPs increased the TP content in plants in comparison to the control, by 25.7% and 16.6%, respectively. Changes in TP content only partially corresponded with the total antioxidant capacity (TAC) of treated lettuce plants (Figure 3B). An increase in TAC was noted for the plants treated with higher concentrations of CeO2-NPs, TiO2-NPs, and Fe2O3-NPs. Additionally, SiO2-NPs caused an increase in TAC, but was used only at 1.5% and 6% concentrations. The greatest difference between control and treated plants was found for plants treated with 3% CeO2-NPs, when the TAC was almost twice as high as in untreated plants.
The content of the tested metals/metalloid in plant tissues increased on increasing the concentration of MO-NPs in the treatment suspension (Table 2); however, significant differences were often found only for the highest NP concentrations applied, compared to the control. Only a trace content of cerium was found in the control lettuce, while foliar spraying with CeO2-NPs at concentrations of 1.5% and 3% increased its content significantly, ca. 3900- and 5500-fold, respectively. Such changes in Fe content were not so spectacular, although significant for the 1.5% and 3% Fe2O3-NPs: ca. 28-, 47-, and 170-fold higher for 0.75%, 1.5%, and 3%, respectively, compared to control plants. In the case of SiO2-NP treatment, the Si content in the plants was statistically similar to that of the control, even if suspension with 1.5%, 3%, and 6% of this NP was used (these slight increases were in the range 1.1- to 1.7-fold). The Sn content in plants increased significantly, ca. 215- and 333-fold compared to the control, when 1.5% and 3% suspensions of SnO2-NPs, respectively, were used. An increase in Ti content in oakleaf lettuce was found, ca. 144-, 320-, and 647-fold for 1.5%, 3%, and 6% suspensions of TiO2-NPs, respectively, in comparison to the control, although only differences for 3% and 6% suspensions were significant.
In order to substantiate the effects of MO-NPs applied at different concentrations, the entire dataset concerning antioxidants was analyzed using a PCA-based clustering approach (Figure 4). As a result of the PCA, 87.17% of the total variation was explained by the first two principal components (PC1 and PC2; 49.79% and 37.38%, respectively). The first principal component (PC1) represents mainly enzymes (APX, GPOX, CAT) and L-ascorbic acid content (values of principal components coefficients ranged from −0.805 to −0.941), and the second principal component (PC2) represents mainly non-enzymatic antioxidants (glutathione, carotenoids, phenolics; coefficients from 0.803 to 0.889). Weights of the seven variables with respect to the first two principal components of the PCA analysis are as follows (PC1 and PC2, respectively): APX—0.796 and 0.881; GPOX—0.886 and 0.922; CAT—0.648 and 0.916; L-ascorbic acid—0.751 and 0.760; glutathione—0.295 and 0.939; phenolics—0.106 and 0.897; carotenoids—0.003 and 0.785. All tested concentrations of Fe2O3-NPs were located on the bottom-left side of the plot with negative factor loadings. The control, CeO2-NPs, and SnO2-NPs had high positive factor loadings with the first component, but negative loadings for PC2, while the SiO2 (1.5% and 6%) and TiO2 (6%) factor loadings were negative for PC1 but positive for the second component. In the case of TiO2-NPs (1.5% and 3%) and SiO2-NPs (3%), both factor loadings were positive. The bottom-left pool with Fe2O3-NPs illustrates the experimental combinations that most significantly influenced the antioxidant status of the plants. The bottom-right pool consisted of treatments with a relatively small effect on antioxidant changes in oakleaf lettuce seedlings; it is worth noting that 3% CeO2-NPs had a greater influence than 1.5% CeO2-NPs. The next two pools contained SiO2-NPs and TiO2-NPs, with a clear demonstration that the greatest effect of these NPs was observed for the highest concentration.

4. Discussion

Several studies have shown that MO-NPs may have both positive and negative effects on plant fresh and dry biomass [24]. The authors mentioned a reduction in shoot biomass of wheat treated with CuO-NPs and ZnO-NPs, cucumber and alfalfa treated with ZnO-NPs, and cotton exposed to SiO2-NPs and CeO2-NPs; ZnO-NPs treatment caused a decrease in the dry weight (DW) of rapeseed and Indian mustard. On the other hand, SiO2-NPs or CeO2-NPs increased the DW of maize and barley plants. Zheng et al. [11] observed a significant increase in the fresh weight (FW) and DW of spinach seedlings treated with TiO2-NPs. In our study, the highest concentration of Fe2O3-NPs reduced the FW of the lettuce seedlings; all concentrations of these MO-NPs increased DW (g 100 g−1 FW). However, total DW (g per shoot) was unaffected by any NPs treatment. Feizi et al. [25] showed that the shoot weight of wheat seedlings was not affected by Fe2O3-NPs. In contrast, Jeyasubramanian et al. [26] noted a significant increase in spinach biomass (FW and DW) when Fe2O3-NPs (from 100 to 200 mg L−1) were applied to a hydroponic medium. These authors concluded that Fe-NPs could be a source of iron for plants when nanoparticles are used at low application rates. In our study, oakleaf lettuce sprayed with Fe2O3-NPs, especially when the highest concentration of these NPs was applied, suffered from stronger abiotic stress than plants of other treatments—there were a higher level of antioxidant mechanism performance as is shown in further parts of this paper, these plants had smaller leaves, with emerging necrosis spots and they had less water (higher content of dry weight per 100 g FW together with no affected total dry weight). All together this had to limit the increase in fresh weight and total dry weight of the plants.
We observed an increase in the total chlorophyll content in oakleaf lettuce treated with SiO2-NPs (1.5% and 6%), TiO2-NPs (3%), and Fe2O3-NPs (3%). Servin et al. [27] found an increase in total chlorophyll content in cucumber leaves when plants were cultivated in soil amended with 750 mg TiO2-NPs per kilogram of soil. Additionally, Zheng et al. [11] reported an increase in chlorophyll content in spinach treated with TiO2-NPs, but only up to a concentration of 2.5‰; above this concentration, the chlorophyll level was reduced. TiO2-NPs may promote photosynthesis due to activation of photochemical reactions in chloroplasts, protect chloroplasts from aging, and increase the formation of chlorophyll, Rubisco activity, and photosynthesis intensity [28]. Karimi and Mohsenzadeh [29] showed for wheat seedlings that at lower concentrations (50 and 100 mg L−1), SiO2-NPs had no negative effects on photosynthetic pigments; however, SiO2-NPs applied at high concentrations decreased the chlorophyll content. According to Sun et al. [30], Si is able to stimulate the expression of genes related to chlorophyll biosynthesis and improve the activity of photosystem II and the electron transfer rate, resulting in an increase in chlorophyll concentration. Results of our study confirm the positive role of SiO2-NPs and TiO2-NPs (in specific concentrations) on chlorophyll content, which is in agreement with most of the literature data. However, chlorophyll increase did not correlate with increasing concentration of SiO2-NPs, which is difficult to explain. We also observed higher chlorophyll content in plants treated with 3% Fe2O3-NPs. This was a surprise, because the high performance of antioxidant mechanism, lower fresh weight and some other symptoms showed by that plants pointed to Fe2O3-NPs as a stress stimulus. Perhaps we should look at the physiological functions of Fe in plants. Wang et al. [31] noted that nano-ferric oxide applied to the substrate on which watermelon seedlings grew, in lower doses (20–50 mg L−1), increased chlorophyll level, the authors explained this phenomenon by involvement Fe in the biosynthesis of chlorophyll and its precursors. Several Fe-dependent steps in the chlorophyll biosynthesis have been reported: the conversion of coproporphyrinogen to protoporphyrin IX, Mg-protoporphyrin IX to protochlorophyllide-monomethyl ester and 5-aminolevulinic acid (ALA, tetrapyrrole precursor) synthesis [32,33]. Wang et al. [34] described for muskmelon treated with iron nanoparticles a time-dependent trend of changes in chlorophyll content compared with control; these authors explained a decrease in chlorophyll concentration in first phase by oxidative stress that caused pigment degradation, and the increase in chlorophyll content in third week by absorption of Fe which is a micronutrient mobilized from applied γ-Fe2O3-NPs and Fe3O4-NPs and its involvement in various processes occurred in plants.
The ROS induced by NPs can cause oxidative stress in plants; to cope with this stress, plants may activate several antioxidant enzymes which are able to scavenge ROS [1,10]. In our experiment, the strongest plant reaction associated with an increase in the activity of antioxidant enzymes was observed for Fe2O3-NPs, usually with increasing concentration. In agreement with our results, Ghafari and Razmjoo [35] reported that foliar application of nano-iron oxide to wheat increases APX activity. Li et al. [9] showed an increase in peroxidase (POD) activity in corn leaves when plants were treated with 20 mg L−1 γ-Fe2O3-NPs, but at higher concentrations, POD activity was lower or similar to the control. These NPs also caused up-regulation of CAT enzyme activity in corn leaves; the relationship with concentration was similar to that for POD. In our study the highest activity of antioxidant enzymes were observed usually when the highest concentration of Fe2O3-NPs was applied to the lettuce plants. Siddiqi and Husen [36] presented in their review the antioxidant enzyme response to NPs treatment, and showed that CeO2-NPs increased APX activity in roots of coriander, but such a stimulatory effect of CeO2-NPs was not observed in our study, moreover, a concentration of 1.5% CeO2-NPs significantly reduced APX activity in lettuce leaves. We did not observe a significant effect of TiO2-NPs on the activity of CAT in lettuce, however, APX activity decreased at 1.5% and 3% TiO2-NPs, while GPOX activity increased when 6% TiO2-NPs was applied. As we can see in the next parts of this report, scavenging of ROS in lettuce plants treated with TiO2-NPs was mainly performed by non-enzymatic antioxidants. Mohammadi et al. [37] showed similar to control plants activity of ascorbate peroxidase and guaiacol peroxidase in the seedlings of one chickpea genotype while APX activity decreased in another tested genotype, both were treated with 5 mg L−1 TiO2-NPs. In cucumber, CAT activity increased for all TiO2-NPs treatments but APX decreased at high concentrations [27]. Our results also showed some modulation of GPOX activity due to SiO2-NPs, but it was strictly dependent on the concentration. An interesting observation was the reduction of APX and GPOX activities due to the treatment of plants with all SnO2-NPs concentrations, and the increase of CAT activity when 3% SnO2-NPs were applied; the mechanism of these changes remains unclear, it may result from the delicate balance between the action of these enzymes in the detoxification of ROS [38].
In almost all treatments of our experiment, with the exception of SnO2-NPs and 1.5% Fe2O3-NPs, content of glutathione, being important non-enzymatic ROS scavenger, increased in oakleaf lettuce plants. A significant difference in GSH concentration between control lettuce and plants exposed to TiO2-NPs was described by Larue et al. [13]; treated plants contained about two-fold more of this compound. This is in agreement with our findings which showed a significant increase in GSH content in plants treated with these nanoparticles. On the other hand, Jahani et al. [39] did not find any changes in GSH in marigold leaves up to a concentration of 200 mg L−1 CeO2-NPs; at higher CeO2-NPs doses, the GSH content gradually decreased, however, our results showed significant increase in GSH content in plants treated with CeO2-NPs. Our data confirm the role of glutathione in overcoming oxidative stress caused by TiO2-NPs and CeO2-NPs. In our study, the GSH level in oakleaf lettuce plants treated with 0.75% and 3% Fe2O3-NPs was higher than in the control; however, Yoganandham et al. [40] showed a substantial depletion of GSH level in Chlorella vulgaris exposed to different concentrations of Fe2O3-NPs, but only after 72 h, not after 24 h. They argued that the decrease in GSH may be attributed to strong oxidative stress caused by Fe2O3-NPs and the consumption of a large amount of GSH in a variety of detoxification processes. We observed an increase in GSH level due to SiO2-NPs treatment. Tripathi et al. [41] also found a higher GSH content in two maize cultivars treated with SiO2-NPs. These authors suggests an important role of SiO2-NPs in triggering up-regulation of antioxidant defense system in plants, but the exact mechanism of action of these nanoparticles is not much known.
Rico et al. [42] observed a similar level of ascorbate in rice seedlings exposed to CeO2-NPs in comparison to the control, as in our experiment; however, those authors reported a higher total ascorbate content for treatment with the highest concentration of CeO2-NPs (500 mg L−1). In the present experiment, higher concentrations of SiO2-NPs caused an increase in L-ascorbic acid (LAA) content; this is similar to the results of Tripathi et al. [41] for maize cultivars, where the plants had a higher total ascorbate content due to SiO2-NPs treatment. Again, silicon’s ability to regulate antioxidant mechanisms in plants is worth emphasizing [43]. We found a significant increase in LAA content due to TiO2-NP treatment. Silva et al. [44] documented total ascorbate changes in wheat leaves and found an increase in the level of this compound for all TiO2-NP treatments (5, 50, and 150 mg L−1). These authors pointed that biosynthesis of thiols and ascorbate as well as AsA-GSH cycle are favored in wheat leaves to face TiO2-NP toxicity. The large role of non-enzymatic antioxidants with cope the oxidative stress caused by TiO2-NPs has also been observed in our experiment. We also found an increase in L-ascorbic acid in plant treated with 0.75% and 3% Fe2O3-NPs. Tavallali [45] noted a higher ascorbic acid content in the aerial parts of purslane when plants were exposed to Fe(III)–aminolevulinic acid (Fe-ALA) nano-complex in comparison to the control.
In the present experiment, the carotenoid content in oakleaf lettuce increased for all concentrations of TiO2-NPs, which is consistent with the results obtained by Mohammadi et al. [37] who noticed that spraying with TiO2-NPs triggered an increase in the carotenoid level in both genotypes of chickpea seedlings compared to control plants. On the other hand, Larue et al. [13] did not find any effect of foliar exposure of lettuce plants to TiO2-NPs on carotenoid concentration. Our results showed an increase in these pigments in oakleaf lettuce due to leaf exposure to SiO2-NPs which is consistent with the data presented by Sharifi-Rad et al. [46] who found an increase in the carotenoid content in maize, common bean, hyssop, and black cumin plants treated with SiO2-NPs up to a concentration of 2000 mg L−1. High level of carotenoids in oakleaf lettuce plants treated with 3% Fe2O3-NPs correlates with overall activity of antioxidant enzymes and higher content of other non-enzymatic antioxidants in plants of that treatment.
The results presented proved that a higher concentration of CeO2-NPs applied to oakleaf lettuce caused an increase in total phenolic (TP) content, which is in agreement with the results of Ma et al. [47] who observed a significant increase in anthocyanin synthesis in Arabidopsis thaliana treated with 1000 and 2000 mg L−1 CeO2-NPs, while no pigment production was evident at 250 mg L−1 treatment. Jahani et al. [39] showed for marigold leaves that the TP content displayed no remarkable variation at 50, 100, and 200 mg L−1 CeO2-NPs but was enhanced at 400 mg L−1 and peaked at 1600 and 3200 mg L−1. In our experiment, all concentrations of SiO2-NPs up-regulated the synthesis of phenolics, which is similar to the results of Farhangi-Abriz and Torabian [48] who observed that phenolic compounds were enhanced in soybean leaves exposed to SiO2-NPs, regardless of dose. Vega et al. [49] point to silicon as an agent enhancing biosynthesis of phenolic compounds in barley. We observed that Fe2O3-NPs at the highest concentration elevated the TP level in oakleaf lettuce. As was shown by Tavallali [45], purslane plants supplied with an Fe-ALA nano-complex at different concentrations had a higher phenolics content than control plants. Foliar exposure of oakleaf lettuce to TiO2-NPs caused in general an increase in TP; similarly Ghorbanpour [50] found that TiO2-NPs elevated the level of these compounds (and flavonoids) in common sage at all tested concentrations.
Jahani et al. [39] detected remarkable differences in DPPH• scavenging activity in marigold at the higher CeO2-NPs concentrations tested (400 to 3200 mg L−1); this increment reached up to 18% compared to the control. Greater total antioxidant capacity in oakleaf lettuce plants treated with the highest concentration of CeO2-NPs was also observed in our experiment, moreover, 6% TiO2-NPs influenced DPPH radical scavenging activity similarly. Higher concentrations of Fe2O3-NPs (1.5% and 3%) caused an increase in antioxidant capacity of oakleaf lettuce. This is in agreement with the finding of Tavallali [45] who claimed higher antioxidant activity in purslane plants treated with Fe-ALA nano-complex, whereas the lowest was found in the control group. The effects of SiO2-NPs (1.5% and 6%, but not 3%) were noted in this respect which is partially in agreement with Torabzadeh et al. [51] who observed that the antioxidant capacity of chamomile extracts increased with an increasing concentration of SiO2-NPs. Explaining the absence of significant differences between control plants and those treated with a 3% SiO2-NPs suspension requires further study.
Zhang et al. [52] noticed that the Ce concentrations in cucumber root and shoot samples increased with increasing concentrations of CeO2-NPs in the nutrient solutions, especially when smaller ceria particles were used. The Fe content increases in a dose-dependent manner in plants treated with Fe2O3-NPs in spinach [26] or with Fe-ALA nano-complex in purslane [45]. Le et al. [53] claimed for Bt-transgenic cotton that the Si content in plant tissues increases with increasing SiO2-NP concentration, which was not observed in our experiment on oakleaf lettuce. A dose–response fashion of Ti accumulation in wheat shoots is evident under TiO2-NP treatment [54]. No such data are available for Sn.

5. Conclusions

In the present study, we evidenced the significant response of the antioxidant mechanism of oakleaf lettuce plants treated with NPs via leaves but it was dependent strongly on the concentration and type of NP applied. An increase in the activity of antioxidant enzymes or in the concentration of antioxidant compounds proved the efficiency of penetration of NPs into the leaves, which caused changes in plant metabolism associated with the occurrence of abiotic stress. The severity of stress depended, however, on the NPs used and their concentrations. It should be emphasized in particular that Fe2O3-NPs generally increased the activity of APX, GPOX, and CAT, as well as the GSH, LAA, carotenoid, and total phenolic content, and the total antioxidant activity of lettuce plants. The result of a significant effect of Fe on most internal parameters should be linked to the most important role of iron in plant physiology compared to the other elements tested. An increase in the content of non-enzymatic antioxidative compounds was also clearly visible after the use of TiO2-NPs and SiO2-NPs, or in the case of GSH and TP for CeO2-NPs. The smallest changes in non-enzymatic compounds in plants occurred due to the use of SnO2-NPs. However, SnO2-NPs decreased activity of APX and GPOX, while CAT activity was elevated. Tin showed a very interesting result which should help in understanding its role in plant stress physiology. The type of research undertaken allows us to better understand the fate of NPs in the environment and biological systems. However, more studies are still needed concerning the plant’s antioxidant system and overall stress response when exposed to various NPs, and such information will be useful for ecological and human health risk assessments.

Author Contributions

R.J. and A.K. performed the experiment and wrote the manuscript; S.S. carried out the analysis of metals/metalloid in plant samples; A.S. and S.S. helped in the writing of some parts of the manuscript; A.S. and R.P. critically evaluated the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work was financially supported by the Ministry of Science and Higher Education of the Republic of Poland. This paper was also supported by project CZ.02.1.01/0.0/0.0/16_017/0002334 of the Research Infrastructure for Young Scientists, co-financed from the Operational Program for Research, Development and Education, and the Ministry of Education of the Czech Republic.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Activity of ascorbate peroxidase (A), guaiacol peroxidase (B), and catalase (C) in oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
Figure 1. Activity of ascorbate peroxidase (A), guaiacol peroxidase (B), and catalase (C) in oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
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Figure 2. Content of glutathione (A), L-ascorbic acid (B), and carotenoids (C) in oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
Figure 2. Content of glutathione (A), L-ascorbic acid (B), and carotenoids (C) in oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
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Figure 3. Total phenolic content (A) and total antioxidant capacity (B) of oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
Figure 3. Total phenolic content (A) and total antioxidant capacity (B) of oakleaf lettuce seedlings treated with engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water. * Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means (n = 3) for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Bars represent standard deviations (± SD).
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Figure 4. Results of principal component analysis (PCA) analysis for experimental treatments based on the activity of antioxidant enzymes and content of non-enzymatic antioxidant compounds in oakleaf lettuce seedlings.
Figure 4. Results of principal component analysis (PCA) analysis for experimental treatments based on the activity of antioxidant enzymes and content of non-enzymatic antioxidant compounds in oakleaf lettuce seedlings.
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Table
Table 1. Fresh and dry weight of oakleaf lettuce seedlings, content of chlorophyll pigments in the plants, and Chl a:Chl b and Car:Chls ratios depending on engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water.
Table 1. Fresh and dry weight of oakleaf lettuce seedlings, content of chlorophyll pigments in the plants, and Chl a:Chl b and Car:Chls ratios depending on engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water.
MO-NPs and ConcentrationFresh Weight
(g per shoot)		Dry Weight
(g 100 g−1 FW)		Total Dry Weight
(g per shoot)		Chlorophyll a
(mg g−1 FW)		Chlorophyll b
(mg g−1 FW)		Chlorophyll a + b
(mg g−1 FW)		Chl a:Chl b
Ratio		Car:Chls Ratio
CeO21.5%2.850 ± 0.2623.309 ± 0.2260.094 ± 0.0110.322 ± 0.0360.134 ± 0.0110.456 ± 0.0412.403 ± 0.2900.149 ± 0.016
3%2.851 ± 0.2633.596 ± 0.0900.103 ± 0.0120.301 ± 0.0330.125 ± 0.0190.426 ± 0.0462.408 ± 0.2820.143 ± 0.017
Fe2O30.75%2.821 ± 0.3153.957 ± 0.196 *a0.111 ± 0.0080.272 ± 0.009 a0.105 ± 0.007 a0.377 ± 0.007 a2.591 ± 0.2310.152 ± 0.019
1.5%2.629 ± 0.4763.999 ± 0.073 *a0.105 ± 0.0170.290 ± 0.006 a0.117 ± 0.002 ab0.407 ± 0.005 a2.479 ± 0.0960.149 ± 0.005
3%2.208 ± 0.386 *4.567 ± 0.276 *b0.100 ± 0.0150.333 ± 0.029 *b0.125 ± 0.009 b0.458 ± 0.038 *b2.664 ± 0.0530.149 ± 0.004
SiO21.5%3.305 ± 0.4073.471 ± 0.0150.115 ± 0.0150.431 ± 0.025 *b0.137 ± 0.008 *b0.568 ± 0.034 *b3.146 ± 0.048 *0.195 ± 0.016 *b
3%3.325 ± 0.0573.603 ± 0.0450.120 ± 0.0010.335 ± 0.023 *a0.107 ± 0.006 a0.442 ± 0.029 a3.131 ± 0.076 *0.177 ± 0.002 *a
6%3.169 ± 0.3253.637 ± 0.2330.116 ± 0.0190.431 ± 0.043 *b0.136 ± 0.014 *b0.567 ± 0.057 *b3.169 ± 0.075 *0.178 ± 0.003 *a
SnO21.5%3.069 ± 0.1213.305 ± 0.0990.102 ± 0.0070.304 ± 0.0370.117 ± 0.0080.421 ± 0.0442.598 ± 0.1670.154 ± 0.009
3%2.993 ± 0.3253.229 ± 0.0080.097 ± 0.0080.285 ± 0.0460.113 ± 0.0130.398 ± 0.0592.522 ± 0.1180.154 ± 0.006
TiO21.5%3.294 ± 0.5583.530 ± 0.1040.116 ± 0.0170.384 ± 0.0020.135 ± 0.0200.519 ± 0.0182.844 ± 0.4070.172 ± 0.002 *
3%3.413 ± 0.5813.602 ± 0.1960.123 ± 0.0220.424 ± 0.064 *0.137 ± 0.0210.561 ± 0.084 *3.095 ± 0.087 *0.173 ± 0.010 *
6%3.131 ± 0.3703.314 ± 0.1940.104 ± 0.0160.380 ± 0.1170.125 ± 0.0420.505 ± 0.1593.040 ± 0.111 *0.167 ± 0.004 *
Control3.027 ± 0.2133.347 ± 0.2340.101 ± 0.0040.270 ± 0.0180.110 ± 0.0150.380 ± 0.0322.455 ± 0.1910.143 ± 0.007
* Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Each value represents the mean (n = 3) ± standard deviation. Chl a—chlorophyll a, Chl b—chlorophyll b, Chls—sum of chlorophyll a and b, Car—total carotenoids.
Table
Table 2. Content of Ce, Fe, Si, Sn, and Ti (ppb in extracts) in oakleaf lettuce seedlings depending on engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water.
Table 2. Content of Ce, Fe, Si, Sn, and Ti (ppb in extracts) in oakleaf lettuce seedlings depending on engineered nanoparticles (nano-metal/metalloid oxides; MO-NPs) applied to the leaves at different concentrations. Control plants were sprayed with deionized water.
MO-NPs and ConcentrationElement Content
Ce
Control0.13 ± 0.02
CeO21.5%512.74 ± 49.49 *a
CeO23%712.94 ± 128.10 *b
Fe
Control33.16 ± 28.52
Fe2O30.75%936.42 ± 117.34 a
Fe2O31.5%1566.10 ± 195.63 *a
Fe2O33%5642.71 ± 1390.65 *b
Si
Control59.13 ± 12.37
SiO21.5%62.42 ± 0.40
SiO23%92.09 ± 51.54
SiO26%97.68 ± 1.69
Sn
Control0.97 ± 0.13
SnO21.5%208.87 ± 2.50 *a
SnO23%323.08 ± 94.94 *b
Ti
Control1.44 ± 0.20
TiO21.5%208.44 ± 36.39 a
TiO23%460.80 ± 89.45 *a
TiO26%932.06 ± 445.18 *b
* Denotes significant differences between particular treatments and unexposed control, comparisons were performed by Fisher test (p ≤ 0.05). Means for concentrations of given MO-NPs followed by different letters are significantly different at p ≤ 0.05 according to Fisher test. Each value represents the mean (n = 3) ± standard deviation.

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Jurkow, R.; Sękara, A.; Pokluda, R.; Smoleń, S.; Kalisz, A. Biochemical Response of Oakleaf Lettuce Seedlings to Different Concentrations of Some Metal(oid) Oxide Nanoparticles. Agronomy 2020, 10, 997. https://doi.org/10.3390/agronomy10070997

AMA Style

Jurkow R, Sękara A, Pokluda R, Smoleń S, Kalisz A. Biochemical Response of Oakleaf Lettuce Seedlings to Different Concentrations of Some Metal(oid) Oxide Nanoparticles. Agronomy. 2020; 10(7):997. https://doi.org/10.3390/agronomy10070997

Chicago/Turabian Style

Jurkow, Rita, Agnieszka Sękara, Robert Pokluda, Sylwester Smoleń, and Andrzej Kalisz. 2020. "Biochemical Response of Oakleaf Lettuce Seedlings to Different Concentrations of Some Metal(oid) Oxide Nanoparticles" Agronomy 10, no. 7: 997. https://doi.org/10.3390/agronomy10070997

APA Style

Jurkow, R., Sękara, A., Pokluda, R., Smoleń, S., & Kalisz, A. (2020). Biochemical Response of Oakleaf Lettuce Seedlings to Different Concentrations of Some Metal(oid) Oxide Nanoparticles. Agronomy, 10(7), 997. https://doi.org/10.3390/agronomy10070997

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