Identification and Functional Characterization of Alfalfa (Medicago sativa L.) Expansins in Regulating Arabidopsis Shoot and Root Development
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification and Characterization of MsEXP Genes in Alfalfa
2.2. Analysis of Chromosomal Distribution and Duplication of MsEXP Genes
2.3. Analysis of Phylogenetic Relationship of MsEXP Proteins
2.4. Gene Structure and Cis-Acting Elements Analysis of MsEXP Genes
2.5. The Conserved Motifs of MsEXP Proteins
2.6. The Organizational Expression Pattern Analysis of MsEXP Genes
2.7. Plant Materials
2.8. RNA Extraction and RT-qPCR Analysis
2.9. Subcelluar Localization Assay
2.10. Scanning Electron Microscopy (SEM) Assay
2.11. Paraffin Section Assay
2.12. Data Processing Method
3. Results
3.1. Characterization, Phylogenetic Analysis and Distribution of Alfalfa Expansins
3.2. Structures and Protein Conserved Motifs of MsEXP Genes
3.3. Cis-Acting Element Analysis of MsEXP Promoters
3.4. The MsEXP Genes Are Differentially Expressed During the Growth and Development of Alfalfa
3.5. MsEXP Proteins Localized in the Cell Wall Implying Their Role in Lossening the Cell Wall
3.6. Overexpression of MsEXPA3 and MsEXPA4 in Arabidopsis Increased the Biomass of Plants by Affecting the Development of Leaf and Stem
3.7. Overexpression of MsEXPA3 and MsEXPA4 in Arabidopsis Also Affects Root Development
3.8. Cell Expansion Was Promoted in MsEXPA3 and MsEXPA4 Overexpression Transgenic Arabidopsis Plants
4. Discussion
4.1. Expansin’s Function in Developmental Progress of Plant Tissues/Organs
4.2. MsEXPA3 and MsEXPA4 May Regulate Organ Size by Increasing Cell Wall Extensibility
4.3. MsEXPA3 and MsEXPA4 Increase Plants’ Biomass by Increasing Cell Size
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Hu, L.; Zhao, M.; Hu, Y.; Xu, Q.; Lu, R. Identification and Functional Characterization of Alfalfa (Medicago sativa L.) Expansins in Regulating Arabidopsis Shoot and Root Development. Agronomy 2024, 14, 2492. https://doi.org/10.3390/agronomy14112492
Hu L, Zhao M, Hu Y, Xu Q, Lu R. Identification and Functional Characterization of Alfalfa (Medicago sativa L.) Expansins in Regulating Arabidopsis Shoot and Root Development. Agronomy. 2024; 14(11):2492. https://doi.org/10.3390/agronomy14112492
Chicago/Turabian StyleHu, Longxing, Mengran Zhao, Yu Hu, Qian Xu, and Rui Lu. 2024. "Identification and Functional Characterization of Alfalfa (Medicago sativa L.) Expansins in Regulating Arabidopsis Shoot and Root Development" Agronomy 14, no. 11: 2492. https://doi.org/10.3390/agronomy14112492
APA StyleHu, L., Zhao, M., Hu, Y., Xu, Q., & Lu, R. (2024). Identification and Functional Characterization of Alfalfa (Medicago sativa L.) Expansins in Regulating Arabidopsis Shoot and Root Development. Agronomy, 14(11), 2492. https://doi.org/10.3390/agronomy14112492