LncR-133a Suppresses Myoblast Differentiation by Sponging miR-133a-3p to Activate the FGFR1/ERK1/2 Signaling Pathway in Goats
, Yang Zhang 2,†, Cuiting Yang 2, Dandan Li 2, Tao Zhong 1,2, Linjie Wang 1,2, Li Li 2 and Hongping Zhang 1,2,*Round 1
Reviewer 1 Report
Comments to the manuscript “LncR-133a suppresses myoblast differentiation by sponging miR-133a-3p to activate the FGFR1/ERK1/2 signaling pathway in goats”
The manuscript provides new and significant information to justify the publication: Here is described for the first time the role of LncR-133a in myoblast differentiation. It is also well written, the literature citation is adequate, the problem was significant and concisely stated, the used methods (qRT-PCR, western blotting, dual-luciferase activity assay, RNA immunoprecipitation, biotin-labeled probe, and RNA fluorescence in situ hybridization) were appropriate to meet the objectives of the research, the tables and figures are clearly illustrated, and the results provide strong evidence about the role of LncR-133a in myoblast differentiation suppression by interacting with miR-133a-3P, thus activating the FGFR1/ERK1/2 signaling pathway in goats.
I only have the following minor comments:
-Line 246: Please indicate the post-hoc test performed after ANOVA.
-Please consider describing in the discussion and conclusion sections the relevance of the study, why is it important to understand the mechanisms of skeletal muscle, the way it was mentioned in the introduction.
Author Response
The manuscript provides new and significant information to justify the publication: Here is described for the first time the role of LncR-133a in myoblast differentiation. It is also well written, the literature citation is adequate, the problem was significant and concisely stated, the used methods (qRT-PCR, western blotting, dual-luciferase activity assay, RNA immunoprecipitation, biotin-labeled probe, and RNA fluorescence in situ hybridization) were appropriate to meet the objectives of the research, the tables and figures are clearly illustrated, and the results provide strong evidence about the role of LncR-133a in myoblast differentiation suppression by interacting with miR-133a-3P, thus activating the FGFR1/ERK1/2 signaling pathway in goats.
Reply: Thanks for your positive comments.
I only have the following minor comments:
-Line 246: Please indicate the post-hoc test performed after ANOVA.
Reply: Thanks for your comments. We have added this information according to your suggestion. Details have been included in the updated manuscript.
-Please consider describing in the discussion and conclusion sections the relevance of the study, why is it important to understand the mechanisms of skeletal muscle, the way it was mentioned in the introduction.
Reply: Thanks for your comments. We rephrased this section according to your suggestion. More details have been included in the updated manuscript.
Reviewer 2 Report
Paper is well-written and explains all the experiments well. As available data on non-coding RNA is very limited so this information is very useful for the researchers working in this area. Hypothesis have been tested through series of relevant experiments and relevant associations have been made to draw meaningful conclusion.
Author Response
Paper is well-written and explains all the experiments well. As available data on non-coding RNA is very limited so this information is very useful for the researchers working in this area. Hypothesis have been tested through series of relevant experiments and relevant associations have been made to draw meaningful conclusion.
Reply: Thanks for your positive comments.
