A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models
Abstract
:1. Introduction
2. Case Presentation
3. Methods
3.1. Animals
3.2. Patient-Derived Xenograft (PDX)
3.3. Monolayer and 3D Cell Cultures
3.4. Chemoresistance Platform
3.5. Immunohistochemistry and Immunofluorescence
3.6. Exome Sequencing and Analysis
4. Results
5. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sousa, A.C.d.S.; Fernandes, B.L.N.C.; da Silva, J.P.A.; Stevanato Filho, P.R.; Coimbra, L.B.d.C.T.; de Oliveira Beserra, A.; Alvarenga, A.L.; Maida, G.; Guimaraes, C.T.; Nakamuta, I.M.; et al. A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models. Genes 2024, 15, 424. https://doi.org/10.3390/genes15040424
Sousa ACdS, Fernandes BLNC, da Silva JPA, Stevanato Filho PR, Coimbra LBdCT, de Oliveira Beserra A, Alvarenga AL, Maida G, Guimaraes CT, Nakamuta IM, et al. A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models. Genes. 2024; 15(4):424. https://doi.org/10.3390/genes15040424
Chicago/Turabian StyleSousa, Ariane Cavalcante dos Santos, Bruno Leonardo Nascimento Correa Fernandes, Jeronimo Paulo Assis da Silva, Paulo Roberto Stevanato Filho, Luiza Bitencourt de Carvalho Terci Coimbra, Adriano de Oliveira Beserra, Ana Luiza Alvarenga, Giovanna Maida, Camila Tokumoto Guimaraes, Ingrid Martinez Nakamuta, and et al. 2024. "A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models" Genes 15, no. 4: 424. https://doi.org/10.3390/genes15040424
APA StyleSousa, A. C. d. S., Fernandes, B. L. N. C., da Silva, J. P. A., Stevanato Filho, P. R., Coimbra, L. B. d. C. T., de Oliveira Beserra, A., Alvarenga, A. L., Maida, G., Guimaraes, C. T., Nakamuta, I. M., Marchi, F. A., Alves, C., Lichtenfels, M., de Farias, C. B., Kupper, B. E. C., Costa, F. D., de Mello, C. A. L., Carraro, D. M., Torrezan, G. T., ... dos Santos, T. G. (2024). A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models. Genes, 15(4), 424. https://doi.org/10.3390/genes15040424