Differential Protein Expression Patterns of HOXA13 and HOXB13 Are Associated with Bladder Cancer Progression
, Huzlinda Hussin 2, De-Ming Chau 1, Teng-Aik Ong 3, Rosna Yunus 4, Azad Hassan Abdul Razack 3, Khatijah Yusoff 5,6, Soon-Choy Chan 6,7,* and Abhi Veerakumarasivam 1,6,8,*Round 1
Reviewer 1 Report
The manuscript entitled "Differential Protein Expression Patterns of HOXA13 and HOXB13 Are Associated with Bladder Cancer" Progressionpresents very interesting results. The work is prepared in a clear and logical way. However, as it concerns tumor markers and the relationship with prognosis, the authors should prepare it in accordance with the REportnig recommendations for tumour MARKer prognostic studies (REMARK) guidelines. I recommend adapting the paper to this guidelines, especially since the authors do not provide any limitations to their analyzes while the reference group is not ideally selected. It consisted of samples of other normal tissue and cancer tissue: "FFPE human skin tissue was used as the positive control for HOXA13 IHC analysis, while FFPE human prostate cancer tissue was used as the positive control for HOXB13 IHC analysis". The number of samples qualified to each of the control groups is also not specified. There is also no information on sensitivity, specificity, positive predictive value and negative predictive value. The tests were performed in the tissue and therefore it is necessary to undergo surgery to perform the tests.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
I consider the authors' explanations and the changes introduced to the paper to be sufficient.
