Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling Sites
2.2. DNA Extraction and PCR Amplification
2.3. Sequencing and Phylogenetic Analysis
2.4. Statistical Analysis
3. Results
3.1. Prevalence of Blastocystis in Sheep and Pigs
3.2. Subtype Distribution of Blastocystis in Sheep and Pigs
3.3. Phylogenetic Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Factor | Category | No. Tested | No. Positive | Prevalence% (95% CI) | OR (95% CI) | p-Value |
---|---|---|---|---|---|---|
Region | Qi County | 97 | 32 | 32.99 (23.63–42.35) | 7.04 (3.68–13.46) | p < 0.001 |
Shanyin County | 135 | 31 | 22.96 (15.87–30.06) | 4.26 (2.26–8.04) | ||
Jishan County | 260 | 17 | 6.54 (3.53–9.54) | 1 | ||
Age | ≤6 M | 211 | 34 | 16.11 (11.15–21.07) | 1 | p = 0.939 |
>6 M | 281 | 46 | 16.37 (12.04–20.70) | 1.02 (0.63–1.65) | ||
Total | 492 | 80 | 16.26 (13.00–19.52) |
Factor | Category | No. Tested | No. Positive | Prevalence% (95% CI) | OR (95% CI) | p-Value |
---|---|---|---|---|---|---|
Region | Qi County | 68 | 12 | 17.65 (8.59–26.71) | 7.71 (2.09–28.47) | p < 0.001 |
Shanyin County | 111 | 3 | 2.70 (0–5.72) | 1 | ||
Jishan County | 183 | 36 | 19.67 (13.91–25.43) | 8.82 (2.65–29.38) | ||
Age | ≤6 M | 237 | 44 | 18.57 (13.62–23.52) | 3.84 (1.68–8.81) | p < 0.001 |
>6 M | 125 | 7 | 5.60 (1.57–9.63) | 1 | ||
Total | 362 | 51 | 14.09 (10.50–17.67) |
Host | Factor | Category | No. Positive/Tested | Prevalence (%) | Subtype (n) |
---|---|---|---|---|---|
Sheep | Region | Qi County | 32/97 | 32.99 | ST5 (31), ST10 (1) |
Shanyin County | 31/135 | 22.96 | ST5 (8), ST10 (11), ST14 (11), ST30 (1) | ||
Jishan County | 17/260 | 6.54 | ST5 (1), ST10 (13), ST14 (3) | ||
Subtotal | 80/492 | 16.26 | ST5 (40), ST10 (25), ST14 (14), ST30 (1) | ||
Pig | Region | Qi | 12/68 | 17.65 | ST5 (11), ST1 (1) |
Shanyin | 3/111 | 2.70 | ST5 (2), ST1 (1) | ||
Jishan | 36/183 | 19.67 | ST5 (34), ST1 (2) | ||
Subtotal | 51/362 | 14.09 | ST5(47), ST1(4) | ||
Total | 131/854 | 15.34 | ST5 (87), ST10 (25), ST14 (14), ST1(4), ST30 (1) |
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Wei, C.-N.; Qin, R.-L.; Zhang, Z.-H.; Zheng, W.-B.; Liu, Q.; Gao, W.-W.; Zhu, X.-Q.; Xie, S.-C. Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective. Animals 2023, 13, 2843. https://doi.org/10.3390/ani13182843
Wei C-N, Qin R-L, Zhang Z-H, Zheng W-B, Liu Q, Gao W-W, Zhu X-Q, Xie S-C. Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective. Animals. 2023; 13(18):2843. https://doi.org/10.3390/ani13182843
Chicago/Turabian StyleWei, Chang-Ning, Rui-Lin Qin, Zhen-Huan Zhang, Wen-Bin Zheng, Qing Liu, Wen-Wei Gao, Xing-Quan Zhu, and Shi-Chen Xie. 2023. "Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective" Animals 13, no. 18: 2843. https://doi.org/10.3390/ani13182843
APA StyleWei, C. -N., Qin, R. -L., Zhang, Z. -H., Zheng, W. -B., Liu, Q., Gao, W. -W., Zhu, X. -Q., & Xie, S. -C. (2023). Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective. Animals, 13(18), 2843. https://doi.org/10.3390/ani13182843