Oncological Applications of Photodynamic Therapy in Dogs and Cats
, Karla Menezes Cardoso 1,2,3,4,5,†, Carlos Miguel Marto 3,4,5,6,7, Ricardo Teixo 3,4,5,7, Beatriz Serambeque 3,4,5, Fernando Capela e Silva 2,8, Nuno Alexandre 2,9, Maria Filomena Botelho 3,4,5,6,7 and Mafalda Laranjo 3,4,5,7,*Round 1
Reviewer 1 Report
The manuscript is well written and described. I don't see much reference to BODIPY which are consider as excellent Photosensitises and are called little sister of porphyrin. The author need to cite most current and adequate literature.
Following paper must be cited.
Lucroy, M.D., Long, K.R., Blaik, M.A., Higbee, R.G. and Ridgway, T.D. (2003), Photodynamic Therapy for the Treatment of Intranasal Tumors in 3 Dogs and 1 Cat. Journal of Veterinary Internal Medicine, 17: 727-729. https://doi.org/10.1111/j.1939-1676.2003.tb02509.x
This review article describe the current advancement of photodynamic Therapy for veterinary medicine. Author have described the field with proper citation. The last such review in this field was published in 2013 by J Buchholz. Hence this review article is important to provide an precise and current informative review in this field.
The author talked about the different photosensitizer but mention of photosensitiser BODIPY was rare. BODIPY is a recent development in the field of Photodynamic Therapy. Author should include more reference on it.
The conclusion is appropriate. The shortcoming in photodynamic therapy is well illustrated. For example the protocols are different for the experiment conducted makes it hard to compare one data set to other. Also, the progress in photodynamic therapy in last decade is impressive.
The Authors have cited many articles older then 10 years. More recent reference are required.
Author Response
Thank you for reviewing our manuscript. Your suggestions were valuable and helped improve the manuscript.
The manuscript is well written and described. I don't see much reference to BODIPY which are consider as excellent Photosensitises and are called little sister of porphyrin. The author need to cite most current and adequate literature.
Current information and references to BODIPY were introduced in the Photosensitisers section, such as Kwon et al., 2022, Zhang et al., 2020, Mai et al., 2022 and Cheng et al., 2021.
Following paper must be cited.
Lucroy, M.D., Long, K.R., Blaik, M.A., Higbee, R.G. and Ridgway, T.D. (2003), Photodynamic Therapy for the Treatment of Intranasal Tumors in 3 Dogs and 1 Cat. Journal of Veterinary Internal Medicine, 17: 727-729. https://doi.org/10.1111/j.1939-1676.2003.tb02509.x
Reference is quoted. Corresponds to reference 112.
This review article describe the current advancement of photodynamic Therapy for veterinary medicine. Author have described the field with proper citation. The last such review in this field was published in 2013 by J Buchholz. Hence this review article is important to provide an precise and current informative review in this field.
The author talked about the different photosensitizer but mention of photosensitiser BODIPY was rare. BODIPY is a recent development in the field of Photodynamic Therapy. Author should include more reference on it.
Current references have been included as described above.
The conclusion is appropriate. The shortcoming in photodynamic therapy is well illustrated. For example the protocols are different for the experiment conducted makes it hard to compare one data set to other. Also, the progress in photodynamic therapy in last decade is impressive.
The Authors have cited many articles older then 10 years. More recent reference are required.
Yes. Many of the studies that report treating oncological diseases in dogs and cats with PDT are in this temporal scope. But our review broadly addresses the up-to-date studies that are available in the databases, we can cite, for example, references from the last 5 years: Maruo et al., 2021, Cramer et al., 2020, Rocha et al., 2019, Martano et al., 2019; Maruo et al., 2019, Osaki et al., 2019, Ishigaki et al., 2018, Flickinger et al., 2018 e Musani et al., 2018.
Reviewer 2 Report
The review of Guimarães et al reviews the advantages and potential applications of Photodynamic therapy. It aims to outline the applications of PDT in the possibility of veterinary medicine, from clinical applications to the most recent growths translationally useful to dogs and cats.
The topic is original and relevant to the field. It agreeably discusses its nominal invasiveness, low toxicity and most importantly the absence of carcinogenic or mutagenic effects.
This review can be useful to researchers and clinical veterinary medicine, especially vets working with small animals.
This research work is interesting, the assessment is well structured and clearly written and informative to support their conclusion.
The references are appropriate. The latest reference of 2022 may be included.
Moreira, Leonardo & Lyon, Juliana. (2022). Photodynamic Therapy in Veterinary Medicine: Applications in dogs and cats. Pubvet. 16. 1-4. 10.31533/pubvet. v16n06a1129.1-4.
Tables and figures are clear and informative. Authors have done a good job with the presentation of tables.
· Tumour word may be replaced with tumor throughout (American english)
· Line 202 no hyphen between appropriate.
· Line 206 no hyphen between associate
· Line 207 no hyphen between treatment
· Line 696 no hyphen between photoablation
Author Response
Thank you for revising our manuscript. Your suggestions were valuable and helped improve the work.
The review of Guimarães et al reviews the advantages and potential applications of Photodynamic therapy. It aims to outline the applications of PDT in the possibility of veterinary medicine, from clinical applications to the most recent growths translationally useful to dogs and cats.
The topic is original and relevant to the field. It agreeably discusses its nominal invasiveness, low toxicity and most importantly the absence of carcinogenic or mutagenic effects.
This review can be useful to researchers and clinical veterinary medicine, especially vets working with small animals.
This research work is interesting, the assessment is well structured and clearly written and informative to support their conclusion.
The references are appropriate. The latest reference of 2022 may be included.
Moreira, Leonardo & Lyon, Juliana. (2022). Photodynamic Therapy in Veterinary Medicine: Applications in dogs and cats. Pubvet. 16. 1-4. 10.31533/pubvet. v16n06a1129.1-4.
Thank you. The reference was included.
Tables and figures are clear and informative. Authors have done a good job with the presentation of tables.
- Tumour word may be replaced with tumor throughout (American english)
The word “tumour” was replaced by “tumor”.
- Line 202 no hyphen between appropriate.
It was corrected.
- Line 206 no hyphen between associate
It was corrected.
- Line 207 no hyphen between treatment
It was corrected.
- Line 696 no hyphen between photoablation
It was corrected.
Reviewer 3 Report
The manuscript is well-written and will provide a useful general overview of the field for people interested in this particular topic. It can be accepted after some minor revisions. I would suggest that an additional figure showing the chemical structures of the various photosensitizers used might be a useful addition for some readers. A Jablonski diagram to accompany the discussion of Type 1 and 2 reactions from lines 61-69, and a diagram showing the wavelength dependent absorbance by hemoglobin, water etc that determines the therapeutic window discussed on lines 114-117, are probably always useful inclusions in the context of any review of PDT.
Author Response
Thank you for reviewing our manuscript. Your suggestions were valuable and helped improve the manuscript.
The manuscript is well-written and will provide a useful general overview of the field for people interested in this particular topic. It can be accepted after some minor revisions. I would suggest that an additional figure showing the chemical structures of the various photosensitizers used might be a useful addition for some readers.
A figure was included with the main chemical structures of the photosensitizers used in the treatment of veterinary neoplasms
A Jablonski diagram to accompany the discussion of Type 1 and 2 reactions from lines 61-69,
A figure with the Jablonski diagram and type 1 and 2 reactions scheme was added.
and a diagram showing the wavelength dependent absorbance by hemoglobin, water etc that determines the therapeutic window discussed on lines 114-117, are probably always useful inclusions in the context of any review of PDT.
A figure containing light penetration into tissues and therapeutic window was added.
