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Article

Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

by
Mohamad Fawzi Mahomoodally
1,2,
Kouadio Ibrahime Sinan
3,
Kouadio Bene
4,
Gokhan Zengin
3,*,
Giustino Orlando
5,*,
Luigi Menghini
5,
Serena Veschi
5,
Annalisa Chiavaroli
5,
Lucia Recinella
5,
Luigi Brunetti
5,
Sheila Leone
5,
Paola Angelini
6,
Vit Hubka
7,
Stefano Covino
6,
Roberto Venanzoni
6,
Marie Carene Nancy Picot-Allain
2,
Laura De Lellis
5,
Alessandro Cama
5,8,
Zoltán Cziáky
9,
József Jekő
9 and
Claudio Ferrante
5add Show full author list remove Hide full author list
1
Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
2
Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit 230, Mauritius
3
Department of Biology, Science Faculty, Selcuk Universtiy, Campus, 42130 Konya, Turkey
4
Laboratoire de Botanique et Phytothérapie, Unité de Formation et de Recherche Sciences de la Nature, 02 BP 801 Abidjan 02, Université Nangui Abrogoua, 00225 Abidjan, Cote D’Ivoire
5
Department of Pharmacy, “G. d’Annunzio” University Chieti-Pescara, 66100 Chieti, Italy
6
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06121 Perugia, Italy
7
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, 14220 Prague, Czech Republic
8
Centre on Aging Sciences and Translational Medicine (Ce.S.I-Me.T), G. d’Annunzio University of Chieti-Pescara, 66100 Chieti, Italy
9
Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, Hungary
*
Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(2), 128; https://doi.org/10.3390/antiox9020128
Submission received: 9 January 2020 / Revised: 26 January 2020 / Accepted: 31 January 2020 / Published: 2 February 2020
(This article belongs to the Special Issue Medicinal, Aromatic and Edible Plants: The Link Between Pharmacy, Food and Nutrition)
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Abstract

:
Bridelia species have been used in traditional African medicine for the management of diverse human ailments. In the current work, the detailed phytochemical profiles of the extracts of the stem bark of B. speciosa were evaluated and the antioxidant and enzyme inhibitory properties of the extracts were assessed. The anti-bacterial and anti-mycotic effects of the extracts were evaluated against selected pathogen strains. Additionally, the anti-proliferative effects were studied on the liver cancer HepG2 cell line. Finally, the putative protective effects were assessed on isolated rat liver that was challenged with lipopolysaccharide (LPS). The results revealed the presence of 36 compounds in the ethyl acetate extract, 44 in the methanol extract, and 38 in the water extract. Overall, the methanol extract showed the highest antioxidant activity, particularly in LPS-stimulated rat liver. Additionally, this extract exerted the highest antimycotic effect on C. albicans, whereas the water extract showed a promising anti-proliferative effect on liver cancer HepG2 cells. The methanol extract was also the most active as enzyme inhibitor, against acetylcholinesterase and butyrylcholinesterase. The current study appraises the antioxidant and enzyme inhibition properties of B. speciosa methanol extract and showed that this specie could be a promising source of biologically active phytochemicals, with potential health uses.

1. Introduction

Plants have played a pivotal role in the progress of mankind, being considered as a substantial source of food and medicine. Traditionally used, for their curative properties among different populations of the world, medicinal plants are still considered to provide outstanding curative effects and they remain the most accessible therapeutic approach to a number of ailments. In traditional medicine, herbal remedies are prepared according to “standardized formula” transmitted from elders or shamans. Some of the preparation methods include decoction, infusion, maceration, tinctures, among others which can be administered by different routes, including optical, dermal, oral, nasal, and anal [1]. The WHO has publicized the need for documentation of ethnomedicinal data on plants, being conscious of the wealth of traditional knowledge that is related to medicinal use represents. Ethnomedicinal records make scientific validation easier and also provide rational regarding the use of plants/herbal preparations for the management of specific ailments [2]. Global public interest for plants-derived products has undoubtedly increased and, today, one of the challenges is to provide scientific evidences of claimed biological activity, but also to unlock the potential of underexplored medicinal plants.
Recently, several endeavors have been made to probe for new sources of bioactive compounds from natural raw materials [3,4]. Among them, the bark of plants is one of the most important sources of bioactive compounds, including phenolics, flavonoids, and terpenes. In addition, extracts that were prepared from barks have been reported to possess broad biological activities, such as antioxidant, antimicrobial, or anti-cancer [3,4,5,6,7]. Based on these data, new studies on uninvestigated bark samples, particularly from Africa, might lead to the discovery of novel bioactive compounds for potential uses in the nutraceutical and pharmaceutical industries.
The Bridelia genus consists of approximately 60–70 species distributed in tropical and subtropical regions of the globe, particularly in Asia and Africa [8]. Several species of this genus have been used in traditional medicinal systems for the management of multiple diseases, including diabetes, urinary stones, lumbago, rheumatism, venereal diseases, bronchitis, gastrointestinal problems, cardiac pain, infertility, epilepsy, and diarrhoea, among others [9]. Keeping this in view, the biological efficacy of several Bridelia species has been claimed in several research pieces [10,11,12,13,14,15]. In earlier studies, the chemical profiles of the members of the Bridelia genus have been reported. For example, previous studies have reported the presence of phenolic acids (gallic acid and ellagic acid, etc.), tannins, and flavonoids in several Bridelia species, including B. ferruginea, B. micrhanta, and B. retusa. Such studies also highlighted the importance of the Bridelia genus, which could open avenues for new studies [16,17,18].
As far as our literature search could ascertain, little scientific information was available on B. speciosa. In this perspective, the current work aims at characterizing the stem bark extracts of B. speciosa investigating phytocompounds and elucidating the antioxidant, enzyme inhibitory properties, protective and anti-proliferative effects in experimental models of liver cancer and inflammation.

2. Materials and Methods

2.1. Plant Material and Preparation of Extracts

The plant samples were collected from wild areas in Gontougo region (Nioumassi) of Ivory Coast in 2018 and they were identified by Dr. Kouadio Bene, botanist at the Laboratoire de Botanique et Phytothérapie, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire. A voucher specimen of the plant material was deposited at Selcuk University, Science Faculty (KIS-1005). The stem barks samples were randomly collected from ten plants in a same population. The stem barks samples were taken stripped vertically while using a knife to the limit of the cambium layer. The stem barks were separated and then dried at room temperature for ten days.
One laboratory mill (Retsch Cutting Mill SM 200, Haan, Germany) was used to powder them (about 2 mm). The extraction procedure was conducted following traditional maceration (for ethyl acetate and methanol) and infusion (for water) methods. Briefly, for maceration, 5 g powdered plant samples was stirred with solvents (100 mL) overnight at the room temperature. Subsequently, the solvents were evaporated using a rotary-evaporator. For water extracts, 5 g powdered plants in boiled water (100 mL) was allowed to stand for 20 min. The aqueous extract was then lyophilized and all of the extracts were kept in +4 °C until use.

2.2. Chemicals

The chemicals were purchased from Sigma–Aldrich (Darmstadt, Germany). They were: 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, rutin, caffeic acid, electric eel acetylcholinesterase (AChE) (type-VI-S, EC 3.1.1.7), horse serum butyrylcholinesterase (BChE) (EC 3.1.1.8), galantamine, acetylthiocholine iodide (ATChI), butyrylthiocholine chloride (BTChI) 5,5-dithio-bis(2-nitrobenzoic) acid (DTNB), tyrosinase (EC1.14.18.1, mushroom), glucosidase (EC. 3.2.1.20, from Saccharomyces cerevisiae), amylase (EC. 3.2.1.1, from porcine pancreas), sodium molybdate, sodium nitrate, sodium carbonate, Folin-Ciocalteu reagent, hydrochloric acid, sodium hydroxide, trolox, EDTA, neocuproine, cupric chloride, ammonium acetate, ferric chloride, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), ammonium molybdate, ferrozine, ferrous sulphate hexahydrate, kojic acid and acarbose. All of the chemicals were of analytical grade.

2.3. Phytochemical Composition

The total bioactive compounds were determined colorimetrically, as described previously [19,20,21]. The results were expressed as mg of standard compounds (gallic acid for phenolic; and rutin for flavonoids; caffeic acid for total phenolic acid; catechin for total flavanol and tannins; quillaja for saponins) per g of dried extract. The bioactive profile of the B. speciosa extracts was determined while using a Dionex Ultimate 3000RS UHPLC instrument. All of the analytical and chromatographic details are given in Supplemental Materials. B. speciosa water and methanol extracts (5 µg/mL) were also analyzed for accurate phenolic quantitative determination of epicatechin, catechin, and gallic acid while using a reversed phase HPLC-fluorimetric in gradient elution mode, as recently described [22]. The experimental details are given in Supplemental Materials.

2.4. Determination of Antioxidant and Enzyme Inhibitory Effects

For antioxidant capacity, different test systems, including radical quenching, reducing power, phosphomolybdenum, and ferrous ion chelating, were used. The methods details were described in our earlier paper [23]. Standard trolox and EDTA equivalents were selected as standards to explain results. For enzyme inhibitory effects, key enzymes for global health problems were selected, namely α-amylase and α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase. Similar to the antioxidant assays, standard equivalent method (acarbose for amylase and glucosidase; galatamine for AChE and BChE; kojic acid for tyrosinase) was selected [23]. Experimental details are given in the Supplemental Materials.

2.5. Antimicrobial and Antimycotic Assays

Antibiotic and antimycotic assays were performed according to our previous studies [24,25]. The detailed description is reported in the Supplementary Material.

2.6. Cell lines and Treatments

Human hepatocellular carcinoma HepG2 cells were cultured in DMEM (Sigma, St. Louis, MO, USA) that was supplemented with 10% FBS at 37 °C, 5% CO2. B. speciosa methanol and water extracts were solubilized in phosphate-buffered saline (PBS) by sonication and then centrifuged at 1200 rpm for five minutes to remove the insoluble fraction. Supernatants were filtered through 0.2 μm pore diameter filters (Euroclone).

2.7. Cell Viability Assay

Cell viability was tested by MTT assay [3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Sigma, St. Louis, MO, USA]. Briefly, the HepG2 cells were seeded in 96-well plates (5 × 103 cells/well) and they were treated the following day for 24, 48, or 72 hours with B. speciosa methanol and water extracts at various concentrations as indicated, or with vehicle (control). The MTT assay was performed as previously described [26]. The IC50 values were calculated while using the CompuSyn software.

2.8. Ex Vivo Studies

Sprague–Dawley rats (200–250 g) were sacrificed by CO2 inhalation (100% CO2 at a flow rate of 20% of the chamber volume per min) and the liver specimens were immediately collected and then maintained at 37 °C for 4 h in RPMI that was supplemented with E. coli LPS (10 µg/mL). During the incubation period, liver specimens were treated with either the methanol and water extracts of B. speciosa (10–500 µg/mL). Afterwards, the tissues were homogenized in 50 mM perchloric acid solution for biochemical determinations, as following described. The liver dopamine (DA) and 3-hydroxy-kinurenine (3-HK) levels were analyzed through an HPLC apparatus (Jasco PU 2080-plus) coupled to electrochemical detection (ESA Coulochem III). All of the details were given in our earlier paper [27].

2.9. Statistical Analysis

The results are given as mean ± S.D. One-way analysis of variance (ANOVA) with post-hoc Tukey test was conducted to determine significant differences (in total bioactive compounds, antioxidant, and enzyme inhibitory assays) between the extracts. ANOVA coupled to Newman–Keuls post-hoc test was employed for statistical analysis of data in pharmacological in vitro and ex vivo assays. GraphPad Prism 5.01 software was used to perform all of the statistical analyses. The results were considered to be statistically significant at p < 0.05.

3. Results and Discussion

While the term “phenolic compounds” includes a broad group of molecules containing at least one phenol unit, different other subgroups, such as flavonoids, tannins, and phenolic acids, among others, can be defined based on their chemical structures [28]. In the current work, the content of major phytochemical groups from B. speciosa stem bark extracts was evaluated while using spectrophotometric methods and are summarized in Table 1. The best concentration of flavanols, tannins, and saponins was observed in the B. speciosa stem bark methanol extract, whereas the highest flavonoid content was obtained in ethyl acetate extract. Flavonoids encompass a group of secondary metabolites having a distinct polyphenolic structure that consists of 15-carbon skeleton (two phenyl rings and one heterocyclic ring) [29]. On the other hand, flavanols or flavan-3-ols, which represent a popular group of flavonoids, include epicatechin and catechin, and their polymerization products [30]. Phenolic acids, which are the most widely distributed plant non-flavonoid phenolic compounds [31], carboxylic acids derivatives of either benzoic or cinnamic acid skeletons [32], were not identified from the ethyl acetate extract. However, HPLC-MS/MS was employed to more accurately evaluate the phytocomposition of B. speciosa stem bark extracts while considering the limitations of spectrophotometric determination in the assessment of phytocompound composition of herbal extract. With regards to the different extracts, 36 compounds were identified from the ethyl acetate extract, 44 from the methanol and 38 from the water extract of B. speciosa stem bark (Table 2). Ellagic, quinic, shikimic, gallic, and ferulic acids were characterized in all of the extracts, albeit the spectrophotometric determination did not reveal the presence of phenolic acids. Corilagin, an ellagitannin, having [M − H]− at m/z 633 was also characterized from all of the B. speciosa extracts. Mallotinic acid or its isomer, a hydrolyzable tannin [32] having [M − H]− at m/z 801, was identified from the methanol and water extracts. Bruguierol A ([M + H]+ at m/z 191), a dammarane triterpene [33] and Prodelphinidin B ([M − H]− at m/z 609), a polymeric tannin composed of gallocatechin, were characterized from the ethyl acetate and methanol extracts of B. speciosa stem bark. Tryptamine, which is a monoamine alkaloid, having [M − H]− at m/z 161, was identified from the methanol extract of B. speciosa stem bark only. Four unidentified tannins were identified from B. speciosa stem bark methanol extract.
In the current work, radical scavenging, reducing power, and metal chelating assays were used to assess the antioxidant properties of B. speciosa stem bark extracts. This approach is believed to provide more accurate and comprehensive information regarding the antioxidant potential of herbal extracts [34]. The methanol extract was the most active in phosphomolybdenum assay, followed by the water and the ethyl acetate extracts (Table 3). A similar order was also obtained for total phenolic content (methanol extract >water extract >ethyl acetate extract). A positive correlation between concentration of phenols and antioxidant capacity was claimed in other studies, which suggests that high phenolic content could be an index of antioxidant capacity [35,36]. Deleterious effects of free radicals that are mainly caused by their instability and high reactivity lead to lipid, protein, and DNA alterations, thereby triggering diseases [37]. In line with the phosphomolybdenum assay, the methanol extract of B. speciosa stem bark was the most effective scavenger of DPPH (495.45 mg TE/g extract) and ABTS (902.33 mg TE/g extract). Apart from radical scavenging, the electron-donating capacity, as measured in terms of reducing power, is also regarded as an important antioxidant mechanism [38]. The CUPRAC and FRAP are the most common methods for measuring reducing power, in vitro. The FRAP evaluates the ability of the herbal extract to reduce ferric to ferrous, whereas the CUPRAC assay measures the conversion of cupric to cuprous [39]. Likewise, B. speciosa stem bark methanol extract showed the highest reducing activity (1325.89 and 952.68 mg TE/g extract, for CUPRAC and FRAP, respectively). However, the methanol extract (12.98 mg EDTAE/g) was the least active metal chelator. Indeed, transition metals, such as iron, can participate in Fenton reaction, converting hydrogen peroxide that is produced from mitochondrial oxidative respiration, into highly toxic hydroxyl free radical [40]. In the current study, the ethyl acetate extract, rich in flavonoids, showed the highest metal chelating properties (Table 3).
Most of the therapeutic drugs that are clinically available function by inhibiting a specific enzyme [41]. Today, the challenge is to find novel inhibitors that can effectively correct metabolic imbalances, without causing side effects. The ability of B. speciosa stem bark extracts to inhibit cholinesterases, tyrosinase, α-amylase, and α-glucosidase was assessed and is reported in Table 4. The inhibition of cholinesterases, namely acetylcholinesterase and butyrylcholinesterase, remains the main focus for the management of Alzheimer’s disease. Besides, an increasing number of publications and clinical studies substantiates that Alzheimer’s disease and type 2 diabetes might share some pathophysiological similarities [42,43]. As such, type 2 diabetes has been identified as a risk factor for Alzheimer’s disease based on multiple connections [44]. From Table 4, it was noted that B. speciosa stem bark methanol extract exhibited the highest inhibition against acetylcholinesterase (4.98 mg GALAE/g extract) and butyrylcholinesterase (5.14 mg GALAE/g extract). The extracts showed a relatively low inhibition against α-amylase (ranging from 0.59 to 1.20 mmol ACAE/g extract), whereas only the ethyl acetate extract (3.56 mmol ACAE/g extract) actively inhibited α-glucosidase. Tyrosinase inhibition is the main therapeutic strategy for the management of epidermal hyperpigmentation conditions. The methanol extract (157.25 mg KAE/g extract) of B. speciosa stem bark showed highest inhibitory activity against tyrosinase. The observed enzyme inhibitory activity of B. speciosa stem bark methanol extract might be related to more than one phytochemical that is present in the extracts. Interestingly, molecular docking studies have previously shown that mangiferin effectively binds to acetylcholinesterase and butyrylcholinesterase [45]. Ferulic acid was reported to hybridise with quinoline in a competitive manner. Carbazole was reported to be more potent than galantamine, which showed pronounced inhibition against cholinesterases [46,47]. On the other hand, it was also reported that corilagin (IC50 = 1.231 mM) was active against tyrosinase [48]. Mangiferin was reported to exert a non-competitive inhibition of α-glucosidase [49].
The methanol and water extracts were selected for further biological assays based on the results of colorimetric analyses indicating a more promising phytochemical profile in terms of total phenols and antiradical activity. A microbiological study was carried out to investigate the potential anti-fungal and anti-bacterial effects of selected pathogen strains, which are fully described in Supplementary Materials. The anti-microbial effects of both water and methanol extracts were compared with reference drugs and presented in Table 5 and Table 6. The results clearly demonstrated that the extracts were less effective when compared to the reference drugs, namely the anti-mycotics fluconazole and griseofulvin and the anti-bacterial ciprofloxacin. Nevertheless, the methanol extract of B. speciosa displayed anti-mycotic activity on C. albicans (YEPGA 6379) that deserves further investigation. This inhibitory effect is consistent with its major content in total phenolic compounds [24,25], as confirmed by both colorimetric assays (Table 1) and independent HPLC-fluorimetric analysis (Table 7). While considering both the incidence of C. albicans opportunistic infections occurring in liver disorders [50] and the traditional use of Bridelia genus [9], a pharmacological investigation was subsequently performed to explore anti-proliferative effects against the liver cancer HepG2 cell line and protective effects on isolated rat liver specimens challenged with the LPS pro-inflammatory stimulus. The HepG2 cell viability was evaluated through the MTT test, which revealed a stimulatory effect induced by methanol extract, up to 48 hours following treatment (Figure 1). Conversely, the water extract of B. speciosa was able to reduce cell viability in the concentration range (300–500 µg/mL) throughout the 72 hour treatment (Figure 1), thus indicating significant anti-proliferative effects that are consistent with more than one speculation. On one side, the concentration-dependent anti-proliferative effects that were exerted by the water extract could be related to epicatechins [51]. This was, at least in part, confirmed by the mild anti-proliferative effect that was exerted by the sole epicatechin (100 µg/mL) (CTR: 100; Epicatechin: 87.88 ± 4.35). On the other hand, we cannot exclude that the stimulating effect induced by methanol extract depends on its major content in phenolic compounds (Table 1 and Table 7), with particular regards to gallic acid, which could also exert putative pro-oxidant effects [22,52], thus potentially contributing to the maintenance of a microenvironment favorable to the proliferation of a cancer cell line [24]. Protective effects following extract treatment were also evaluated in a toxicological model constituted by isolated liver specimens that were stimulated with LPS, which increased the 3-HK levels in the liver tissue (Figure 2). 3-HK is a kinurenine-3-monooxygenase (KMO)-deriving kinurenine metabolite that is able to induce oxidative stress in multiple tissues, including the brain and pancreas [53,54]. Additionally, increased KMO activity was described in rodent models of acute pancreatitis [53], despite that there is still a lack of scientific evidence about KMO activity and 3-HK level in inflamed liver. Conversely, liver dopamine (DA) levels were reduced in the same experimental condition (Figure 3). Besides its crucial role as neurotransmitter in the central nervous system, recent findings suggest anti-inflammatory and protective effects that are induced by DA administration in experimental models of acute pancreatitis and hepatitis [55,56]. Consistent with the observed antiradical activity, both of the extracts reduced the LPS-induced levels of 3-HK (Figure 2). On the other hand, the water extract was completely ineffective against the LPS-induced levels of DA, thus ruling out the involvement of DA in mediating extract anti-oxidant effects, in the liver. By contrast, the methanol extract displayed a significant stimulating effect on liver DA concentration (Figure 3). This protective effect is consistent with both the antioxidant effect that is exerted by the extract and with literature [22,27]. Overall, the present pharmacological assays suggest that B. speciosa could be considered as a source of natural compounds with potential application in liver inflammation and cancer.

4. Conclusions

This is the first report regarding the biological and phytochemical profiles of B. speciosa stem bark extracts. In this respect, our findings can be considered as the first attempt to provide new scientific information on the Bridelia genus. Among the three extracts studied, the methanol extract showed antioxidant and inhibitory properties against enzymes that are related to Alzheimer’s disease and epidermal hyperpigmentation conditions. The antioxidant effects displayed by the methanol extract were also consistent with the observed protective effects in the liver and the anti-mycotic effect against the C. albicans (YEPGA 6379) strain. The protective effects on rat liver induced by methanol extract were also substantiated by the increased DA and reduced 3-HK levels. On the other hand, the water extract reduced the HepG2 cell viability, thus suggesting potential anti-proliferative effects. Several compounds identified and quantified in the B. speciosa stem bark water and methanol extracts, including gallic acid and catechins, might be responsible for the observed effects. Therefore, the isolation of compounds from the methanol and water extracts is required for the validation of the observed pharmacological investigations. To sum up, our findings suggest that B. speciosa barks may be a key bio-resource for the development of novel pharmaceuticals or cosmeceuticals. Further studies are strongly recommended for exploring more biological properties through in vivo animal studies.

Supplementary Materials

The following are available online at https://www.mdpi.com/2076-3921/9/2/128/s1. Supplementary Materials and Methods; CFF-strains.

Author Contributions

Conceptualization, C.F. and G.Z.; methodology, G.O.; L.M.; software, L.M.; validation,: C.F.; G.Z.; L.M.; G.O.; formal analysis, C.F.; G.Z.; investigation, K.I.S.; K.B.; S.L.; L.R.; A.C. (Annalisa Chiavaroli); S.V.; P.A.; V.H.; S.C.; R.V.; L.D.L.; A.C. (Alessandro Cama); M.C.N.P.-A.; Z.C.; J.J.; M.F.M.; resources, C.F.; G.O.; L.M.; data curation, C.F.; G.Z.; writing—original draft preparation, M.F.M.; writing—review and editing, C.F., G.Z.; G.O.; L.M.; visualization, L.B.; supervision, L.B.; project administration, C.F.; L.M.; G.O.; G.Z.; funding acquisition, C.F.; G.O.; L.M. All authors have read and agreed to the published version of the manuscript.

Funding

The study was supported by Italian Ministry of University funds (FAR 2019) granted to Prof. Claudio Ferrante. The contribution of V.H. was supported by the grant of the Czech Ministry of Health (AZV 17–31269A).

Acknowledgments

The experiments were approved by Local Ethical Committee (University “G. d’Annunzio” of Chieti-Pescara) and Italian Health Ministry (Italian Health Ministry authorization N. F4738.N.XTQ, delivered on 11 November 2018).

Conflicts of Interest

The authors declare no conflict of interest.

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Antioxidants 09 00128 g001 550
Figure 1. B. speciosa methanol and water extracts affect cell viability in human hepatocellular carcinoma HepG2. Cell viability was assessed by MTT assay after incubation for 24, 48, or 72 h, with the extracts at various concentrations as indicated, or with vehicle (control). Data shown are the means + SD of two independent experiments with quadruplicate determinations. Statistical analyses were performed using GraphPad Prism version 5.01 software (San Diego, CA). Comparisons of mean values between control and each drug concentration were performed by an unpaired Student’s t-test. A p-value ≤ 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001).
Figure 1. B. speciosa methanol and water extracts affect cell viability in human hepatocellular carcinoma HepG2. Cell viability was assessed by MTT assay after incubation for 24, 48, or 72 h, with the extracts at various concentrations as indicated, or with vehicle (control). Data shown are the means + SD of two independent experiments with quadruplicate determinations. Statistical analyses were performed using GraphPad Prism version 5.01 software (San Diego, CA). Comparisons of mean values between control and each drug concentration were performed by an unpaired Student’s t-test. A p-value ≤ 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001).
Antioxidants 09 00128 g001
Antioxidants 09 00128 g002 550
Figure 2. Effects of B. speciosa methanol and water extracts on LPS-induced 3-HK level in isolated rat liver specimens. ANOVA, p < 0.0001; ** p < 0.01, *** p < 0.001 vs. LPS control group.
Figure 2. Effects of B. speciosa methanol and water extracts on LPS-induced 3-HK level in isolated rat liver specimens. ANOVA, p < 0.0001; ** p < 0.01, *** p < 0.001 vs. LPS control group.
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Antioxidants 09 00128 g003 550
Figure 3. Effects of B. speciosa methanol and water extracts on LPS-induced DA level in isolated rat liver specimens. ANOVA, p < 0.001; * p < 0.05, ** p < 0.01 vs. LPS control group.
Figure 3. Effects of B. speciosa methanol and water extracts on LPS-induced DA level in isolated rat liver specimens. ANOVA, p < 0.001; * p < 0.05, ** p < 0.01 vs. LPS control group.
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Table
Table 1. Total bioactive components of the tested samples.
Table 1. Total bioactive components of the tested samples.
SamplesTotal phenolic Content (mg GAE/g Extract)Total Flavonoid Content (mg RE/g Extract)Total Phenolic Acid Content (mg CAE/g)Total Flavanol Content (mg CE/g)Total Tannin Content (mg CE/g)Total Saponin Content (mg QE/g)
EA38.42 ± 0.38 c5.85 ± 0.12 and3.61 ± 0.02c 3.28 ± 0.38 c177.82 ± 14.15 c
MeOH224.28 ± 1.08 a1.51 ± 0.04 b11.55 ± 1.31 b246.28 ± 10.63 a324.09 ± 10.99 a1031.45 ± 48.83 a
Water210.29 ± 0.71 b1.44 ± 0.17 b13.91 ± 0.42 a6.15 ± 0.18 b67.83 ± 3.64 b772.56 ± 56.39 b
Values expressed are means ± S.D. of three parallel measurements. GAE: Gallic acid equivalent; RE: Rutin equivalent; CE: catechin equivalent; CAE: caffeic acid equivalent; QE: Quillaja equivalent; EA: Ethyl acetate; MeOH: Methanol; nd: not detected. Different letters indicate significant differences in the extracts (p < 0.05).
Table
Table 2. Chemical composition of the tested extracts.
Table 2. Chemical composition of the tested extracts.
No.NameClass 3FormulaRt ± 0.03 min[M + H]+[M − H]−Fragment 1Fragment 2Fragment 3Fragment 4Fragment 5Detected in Extract 2
1Quinic acidaC7H12O61.23 191.05557173.0447127.0388111.043893.033185.0280A,B,C
2Shikimic acidaC7H10O51.31 173.04500155.0338137.0234111.043993.033173.0280A,B,C
3Citric acidaC6H8O71.57 191.01918173.0082129.0181111.007487.007385.0280B,C
4Prodelphinidin BbC30H26O141.73 609.12444441.083423.073305.0672177.0185125.0231B,C
5 1Gallic acid (3,4,5-Trihydroxybenzoic acid)cC7H6O52.29 169.0137125.023197.028281.033279.017569.0329A,B,C
6Gallocatechin (Casuarin, Gallocatechol)dC15H14O74.52 305.06613261.0767219.0651167.0341137.0234125.0232A,B,C
7 1TryptamineeC10H12N28.44161.107875 144.0810143.0732117.0703115.0546103.0547B,C
8Syringic acid-4-O-glucosidefC15H20O1010.57 359.09783197.0451182.0214153.0546138.031123.0073C
9 1Catechin (Catechol, Catechuic acid)dC15H14O613.28 289.07121245.082203.0711151.0389125.0233109.028B,C
10 1Epigallocatechin (Epigallocatechol)dC15H14O713.57 305.06613261.0767219.0658167.0339137.0234125.0232A,B,C
11 1Vanillin (4-Hydroxy-3-methoxybenzaldehyde)gC8H8O315.47153.05517 125.0601111.0445110.036793.034165.0393A,B,C
12 1Epigallocatechin-3-O-gallate (Teatannin II)dC22H18O1116.39 457.07709305.0661169.0131161.0238125.0231 A,B,C
13 1Gallocatechin-3-O-gallatedC22H18O1116.40 457.07709305.067169.0133161.0233125.0231 C
14Dihydrokaempferol-O-hexosidedC21H22O1117.02 449.10839287.0568269.0447259.0607125.023 A,B,C
15 1EpicatechindC15H14O617.04 289.07121245.0818203.0706151.0388125.0231109.028B,C
163,5-Dimethoxy-4-hydroxybenzaldehyde (Syringaldehyde)gC9H10O417.24183.06574 155.0705140.0469123.0444105.034195.0498A,B
17CorilaginhC27H22O1817.49 633.07279463.0526419.0627300.9995275.0205169.0134A,B,C
18Mangiferin (Aphloiol, Chinonin)iC19H18O1118.41 421.07709343.0459331.0464301.0358272.033259.0249A,B,C
19Unidentified tannin 1hC34H26O2218.96 785.08375633.0741300.9992275.0205125.0229 B
20Ferulic acidcC10H10O419.25 193.05009178.0259149.0594137.023134.0364121.028A,B,C
21Mallotinic acid or isomerhC34H26O2319.28 801.07867757.0872633.0753613.047463.0517300.9995B,C
22 1Epicatechin-3-O-gallatedC22H18O1019.37 441.08218289.0725271.0614245.0808169.0132125.023B,C
23LoliolidejC11H16O319.47197.11777 179.1071161.0963135.1172133.1016107.0861A,B,C
24Unidentified tannin 2hC41H30O2719.63 953.08963300.9994275.02249.0387 B,C
25Ellagic acid-4-O-glucosidekC20H16O1319.90 463.05127300.9995299.9915 A,B,C
264-Hydroxy-3-methoxycinnamaldehyde (Coniferyl aldehyde)gC10H10O319.97179.07082 161.0599147.0443133.0654119.049655.0187A,B,C
27Unidentified tannin 3hC34H26O2220.08 785.08375633.0734300.9994275.0207 B
28Isoferulic acidcC10H10O420.30 193.05009178.0264149.06137.0232134.0362121.0283A
29Unidentified tannin 4hC34H26O2221.25 785.08375300.9996275.0205249.0402125.0228 B,C
30Myricitrin (Myricetin-3-O-rhamnoside)dC21H20O1221.96 463.08765317.0292316.023287.0213271.0255178.9978B,C
31Di-O-methylellagic acid-O-hexosidekC22H20O1322.16 491.08257476.0599328.023312.9996297.9761 A,B,C
32Ellagic acid-O-pentosidekC19H14O1222.76 433.04071300.9994299.9916283.9974257.0082 A,B,C
33Eschweilenol C (Ellagic acid-4-O-rhamnoside)kC20H16O1223.09 447.05636300.9994299.9916 A,B,C
34Pentahydroxyflavone-C-hexosidedC21H20O1223.11465.10331 447.0935429.0806369.0611327.0503303.0504A
35Ellagic acidkC14H6O823.38 300.99845283.9967257.0094229.0138201.0187185.0237A,B,C
36Dimethoxy-trihydroxyflavone-O-hexosidedC23H24O1224.29 491.11895328.0586313.0352299.0195285.0397271.0252B,C
37Di-O-methylflavellagic acid O-hexosidekC21H18O1324.70 507.07749344.0187328.994313.97 A
38Ducheside A (3-O-Methylellagic acid-4′-O-xyloside)kC20H16O1224.74 447.05636315.0151314.0074299.9917298.983270.9886A,B,C
393,3′-Di-O-methylellagic acid-O-pentosidekC21H18O1225.32 461.07201446.0498328.0228312.9995297.9757 A,B,C
403,3′,4-Tri-O-methylflavellagic acid-4-O-glucosidekC23H22O1425.55 521.09314506.0705491.0473358.0327343.0098327.9864A,B,C
41Eschweilenol A or isomerkC20H10O1125.90 425.01449300.9993299.9917298.9837 B
42DihydroactinidiolidejC11H16O226.58181.12286 163.112145.1015135.1172121.1015107.0861A,B,C
43Di-O-methylellagic acid acetylhexosidekC24H22O1427.49 533.09313328.0231312.9999297.9756269.9827 A
443,3′-Di-O-methylellagic acidkC16H10O827.84 329.02975314.0073298.9837270.9887 A,B,C
45Sebacic acidaC10H18O427.96 201.11268183.102157.1229139.1117111.0801 A
463,3′,4-Tri-O-methylellagic acidkC17H12O830.18 343.0454328.0231312.9995297.9758285.0038 A
47Undecanedioic acidaC11H20O430.85 215.12834153.1273125.0956 A
483,3′,4-Tri-O-methylflavellagic acidkC17H12O931.21 359.04031344.0171328.9948313.9717300.9995 A,B,C
493,3′,4,4′-Tetra-O-methylellagic acidkC18H14O832.00359.0767 344.0533343.0448329.0295313.0347 A,B,C
50Dihydroxy-trimethoxyflavonedC18H16O733.10 343.08178328.0585313.0359298.0118 B
51Bruguierol AlC12H14O236.06191.10721 173.0965161.0966147.0801135.0807107.0496A,B
521Linoleic acidaC18H32O245.69 279.23241261.223159.0124 A,B
53Pheophytin AmC55H74N4O562.94871.57375 593.277533.2559460.2264 A,B
1 Confirmed by standard. 2 A: Ethyl acetate extract; B: Methanol extract; C: water extract. 3 a: carboxylic acid; b: polyflavonoid; c: phenolic acid; d: flavonoid; e: alkaloid; f: phenolic acid glucoside; g: phenolic aldehyde; h: tannin; i: xanthon; j: benzofuran; k: benzopyrane; l: phenolic heterocycle; m: porphyrin.
Table
Table 3. Antioxidant activities of the tested samples.
Table 3. Antioxidant activities of the tested samples.
SamplesPhosphomolybdenum
(mmol TE/g)		DPPH
(mg TE/g Extract)		ABTS
(mg TE/g Extract)		CUPRAC
(mg TE/g Extract)		FRAP
(mg TE/g Extract)		Metal Chelating Ability (mg EDTAE/g)
EA2.24 ± 0.07 c18.62 ± 0.39 c14.82 ± 0.45 c94.34 ± 0.82 c46.13 ± 0.58 c32.08 ± 1.60 a
MeOH5.89 ± 0.37 a495.45 ± 0.53 a902.33 ± 2.41 a1325.89 ± 30.05 a952.68 ± 23.61 a12.98 ± 0.10 b
Water5.17 ± 0.14 b463.86 ± 14.04 b581.14 ± 33.94 b1082.42 ± 3.72 b850.05 ± 5.35 b14.28 ± 2.15 b
Values expressed are means ± S.D. of three parallel measurements. TE: Trolox equivalent; EDTAE: EDTA equivalent; EA: Ethyl acetate; MeOH: Methanol. Different letters indicate significant differences in the extracts (p < 0.05).
Table
Table 4. Enzyme inhibitory properties of the tested extracts.
Table 4. Enzyme inhibitory properties of the tested extracts.
SamplesAChE
(mg GALAE/g Extract)		BChE
(mg GALAE/g Extract)		Tyrosinase
(mg KAE/g Extract)		α-Amylase
(mmol ACAE/g Extract)		α-Glucosidase (mmol ACAE/g Extract)
EA4.56 ± 0.20 b3.59 ± 0.05 b119.80 ± 1.30 c0.86 ± 0.03 b3.56 ± 0.03
MeOH4.98 ± 0.04 a5.14 ± 0.08 a157.25 ± 0.48 a1.20 ± 0.01 ana
Water3.60 ± 0.15 c2.61 ± 0.31 c137.49 ± 0.35 b0.59 ± 0.04 cna
Values expressed are means ± S.D. of three parallel measurements. AChE: acetylcholinesterase; BChE: butyrylcholinesterase; GALAE: Galantamine equivalent; KAE: Kojic acid equivalent; ACAE: Acarbose equivalent; na: not active; EA: Ethyl acetate; MeOH: Methanol. Different letters indicate significant differences in the extracts (p < 0.05).
Table
Table 5. Minimal inhibitory concentrations (MICs) of B. speciosa water and methanol extracts, fluconazole, and griseofulvin against clinical yeasts and dermatophytes.
Table 5. Minimal inhibitory concentrations (MICs) of B. speciosa water and methanol extracts, fluconazole, and griseofulvin against clinical yeasts and dermatophytes.
MIC (µg mL−1) *
Fungal Strains Methanol ExtractWater ExtractFluconazoleGriseofulvin
Candida albicans (YEPGA 6183) 396.85 (250–500)198.42 (125–250)2>8
Candida albicans (YEPGA 6379) 49.6 (31.25–62.5)78.74 (62.5–125)1>8
Candida tropicalis (YEPGA 6184) 629.96 (500–1000)396.85 (250–500)4>8
Candida parapsilosis (YEPGA 6551) 78.74 (62.5–125)99.21 (62.5–125)2>8
Arthroderma crocatum (IHEM 5251) 157.49 (125–250)78.74 (62.5–125)8>8
Arthroderma crocatum (CCF 5207) 99.21 (62.5–125)78.74 (62.5–125)>16>8
Arthroderma insingulare (CCF 5417) 157.49 (125–250)39.37 (31.25–62.5)>16>8
Arthroderma quadrifidum (CCF 5792) 198.42 (125–250)78.74 (62.5–125)>16>8
Trichophyton erinacei (CCF 5930) 314.98 (250–500)157.49 (125–250)>160.25
Trichophyton interdigitale (CCF 4823)99.21 (62.5–125)49.61 (31.25–62.5)>161
Trichophyton rubrum (CCF 4879) 78.74 (62.5–125)78.74 (62.5–125)82
Trichophyton tonsurans (CCF 4834) 157.49 (125–250)39.58 (31.25–62.5)20.125
* MIC values are reported as geometric means of three independent replicates (n = 3); MIC range concentrations are reported within brachets. CCF, Culture Collection of Fungi, Department of Botany, Charles University, Prague, Czech Republic; IHEM, Belgian Coordinated Collections of Micro-organisms (BCCM/IHEM), Brussels, Belgium; YEPGA, yeast extract-peptone-glucose agar.
Table
Table 6. Minimum inhibitory concentration (MIC) of B. speciosa extracts and ciprofloxacin towards selected bacterial strains.
Table 6. Minimum inhibitory concentration (MIC) of B. speciosa extracts and ciprofloxacin towards selected bacterial strains.
MIC (µg mL−1) *
Bacterial Strains Methanol ExtractWater ExtractCiprofloxacin
Escherichia coli (ATCC 10536)396.85 (250–500)629.96 (500–1000)<0.12
Pseudomonas aeruginosa (ATCC 15442)629.96 (500–1000)314.98 (250–500)1.23 (1.95–0.98)
Salmonella typhimurium (clinical isolate)793.70 (500–1000)793.70 (500–1000)0.40 (0.25–0.5)
Bacillus cereus (ATCC 12826)198.42 (125–250)157.49 (125–250)<0.12
Bacillus subtilis (environmental isolate)314.98 (250–500)793.70 (500–1000)0.01 (0.125–0.062)
Staphylococcus aureus (ATCC 6538)198.42 (125–250)396.85 (250–500)0.62 (0.98–0.49)
* MIC values are reported as geometric means of three independent replicates (n = 3); MIC range concentrations are reported within brachets.
Table
Table 7. Gallic acid, catechin and epicatechin level (µg/g dry extract) in methanol and water extracts of B. speciosa.
Table 7. Gallic acid, catechin and epicatechin level (µg/g dry extract) in methanol and water extracts of B. speciosa.
CompoundsMethanol ExtractWater Extract
Gallic acid7228.36 ± 650.55870.28 ± 36.81
Catechin20.84 ± 2.51n.d.
Epicatechin188.72 ± 11.32142.71 ± 7.75
n.d.—not determined.

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Mahomoodally, M.F.; Sinan, K.I.; Bene, K.; Zengin, G.; Orlando, G.; Menghini, L.; Veschi, S.; Chiavaroli, A.; Recinella, L.; Brunetti, L.; et al. Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf. Antioxidants 2020, 9, 128. https://doi.org/10.3390/antiox9020128

AMA Style

Mahomoodally MF, Sinan KI, Bene K, Zengin G, Orlando G, Menghini L, Veschi S, Chiavaroli A, Recinella L, Brunetti L, et al. Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf. Antioxidants. 2020; 9(2):128. https://doi.org/10.3390/antiox9020128

Chicago/Turabian Style

Mahomoodally, Mohamad Fawzi, Kouadio Ibrahime Sinan, Kouadio Bene, Gokhan Zengin, Giustino Orlando, Luigi Menghini, Serena Veschi, Annalisa Chiavaroli, Lucia Recinella, Luigi Brunetti, and et al. 2020. "Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf" Antioxidants 9, no. 2: 128. https://doi.org/10.3390/antiox9020128

APA Style

Mahomoodally, M. F., Sinan, K. I., Bene, K., Zengin, G., Orlando, G., Menghini, L., Veschi, S., Chiavaroli, A., Recinella, L., Brunetti, L., Leone, S., Angelini, P., Hubka, V., Covino, S., Venanzoni, R., Picot-Allain, M. C. N., De Lellis, L., Cama, A., Cziáky, Z., ... Ferrante, C. (2020). Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf. Antioxidants, 9(2), 128. https://doi.org/10.3390/antiox9020128

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