Effects of the Dental Caries Preventive Procedure on the White Spot Lesions during Orthodontic Treatment—An Open Label Randomized Controlled Trial
, Toshiko Sekiya 1, Chihiro Arai 1, Ayako Okada 4, Kaoru Sogabe 2, Nobuhiro Hanada 3 and Hiroshi Tomonari 1Round 1
Reviewer 1 Report
Line 64, 65: Please recheck this sentence
Line 62: Please check the reference
Line 83: Please check English grammar in this sentence
Line83: Already mentioned abbreviations, no need to do it here
Line 94: among them
Line 96: Finally completed
Line 102: Can you explain why inclusion criteria was 6 weeks of not using antibiotics. Is it enough time after antibiotics to have accurate microbiological results?
Line 108, 109: Please check English grammar
Author Response
Reply to Reviewer 1
Thank you for your valuable comments to improve our manuscript. We reply to your comments point by point. The changes according to your comments were high lightened yellow in revised manuscript.　Please see attached PDF file.
- Line 64, 65: Please recheck this sentence
<Reply>
We revised the sentences.
<Previous version>
Among the antimicrobial agents available for dental use, CHX is the most commonly used to control cariogenic activity. CHX reduces the proportion of mutans streptococci, which is particularly sensitive to mutans streptococci.
<Revised sentences>
Among the antimicrobial agents available for dental use, CHX, which is particularly sensitive to mutans streptococci, is the most commonly used to control cariogenic activity.
- Line 62: Please check the reference
<Reply>
As the reference was old, following sentence was removed.
In compliant patients with good oral hygiene, orthodontic treatment suggested not progress dental caries [23].
- Line 83: Please check English grammar in this sentence.
The sentence was revised.
<Reply>
<Previous version>
Follow up periods was six months.
<Revised sentences>
Follow up period was six months.
- Line83: Already mentioned abbreviations, no need to do it here.
<Reply>
Abbreviation was removed.
- Line 94: among them
<Reply>
Thank you for pointing out our mistake. The word was revised.
Among them
- Line 96: Finally completed
<Reply>
The sentence was revised.
<Previous version>
A total of 22(8 males, 14 females) patients were finally completed this study.
<Revised sentences>
A total of 22(8 males, 14 females) patients were finally completed.
- Line 102: Can you explain why inclusion criteria was 6 weeks of not using antibiotics. Is it enough time after antibiotics to have accurate microbiological results?
<Reply>
The inclusion criteria of this study was based on the previous report.
References were included.
4) salivary levels of mutans streptococci over 0.2% against total streptococci, 5) not using antibiotics for at least 6 weeks before saliva sampling or during the experimental period [19,22,30,31,32].
- Line 108, 109: Please check English grammar
<Reply>
The sentence was revised.
<Previous version>
According to the difference of mean values and standard deviation of the previous studies [32], sample size to obtain the tooth level intragroup significant difference of C of were 22 to 105 teeth (α=0.05 and power=0.80).
<Revised sentences>
According to the mean values and standard deviations of the previous studies [34], sample size to obtain the tooth level intragroup significant difference of C of was 22 to 105 teeth (α=0.05 and power=0.80).
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Dear Authors,
thank you for your work which I found interesting. I suggest you to improve your discussion by stating the efficacy of biomimetic hydroxyapatite in remineralising the tooth. You can refer to the following article:
Butera A, Pascadopoli M, Gallo S, Lelli M, Tarterini F, Giglia F, Scribante A. SEM/EDS Evaluation of the Mineral Deposition on a Polymeric Composite Resin of a Toothpaste Containing Biomimetic Zn-Carbonate Hydroxyapatite (microRepair®) in Oral Environment: A Randomized Clinical Trial. Polymers (Basel). 2021 Aug 16;13(16):2740. doi: 10.3390/polym13162740. PMID: 34451279; PMCID: PMC8398018.
Yours faithfully.
The Reviewer
Author Response
Reply to Reviewer 2
Thank you for your valuable comments to improve our manuscript. We reply to your comments point by point. The changes according to your comments were high lightened yellow in revised manuscript.
- Thank you for your work which I found interesting. I suggest you to improve your discussion by stating the efficacy of biomimetic hydroxyapatite in remineralising the tooth. You can refer to the following article:
Butera A, Pascadopoli M, Gallo S, Lelli M, Tarterini F, Giglia F, Scribante A. SEM/EDS Evaluation of the Mineral Deposition on a Polymeric Composite Resin of a Toothpaste Containing Biomimetic Zn-Carbonate Hydroxyapatite (microRepair®) in Oral Environment: A Randomized Clinical Trial. Polymers (Basel). 2021 Aug 16;13(16):2740. doi: 10.3390/polym13162740. PMID: 34451279; PMCID: PMC8398018.
<Reply>
As the Discussion constructed by antimicrobial agents and microbiome, your suggestion was included in the limitation. Following sentences were included in the manuscript. Insertes sentence and references are highlighted sky blue.
<Inserted sentences>
In addition, more effective remineralization methods has been proposed[64-68]. For the QOL of patients, more effective remineralization method should be developed in combination of tooth surface disinfection and remineralization.
<Inserted references>
- Butera, A.; Pascadopoli, M.; Gallo, S.; Lelli, M.; Tarterini, F.; Giglia, F.; Scribante, A. SEM/EDS Evaluation of the Mineral Deposition on a Polymeric Composite Resin of a Toothpaste Containing Biomimetic Zn-Carbonate Hydroxyapatite (microRepair®) in Oral Environment: A Randomized Clinical Trial. Polymers (Basel). 2021, 13, 2740. DOI: 10.3390/polym13162740. PMID: 34451279
- Ionescu, A.C.; Cazzaniga, G.; Ottobelli, M.; Garcia-Godoy, F.; Brambilla, E. Substituted Nano-Hydroxyapatite Toothpastes Reduce Biofilm Formation on Enamel and Resin-Based Composite Surfaces. J Funct Biomater. 2020, 11, 36. DOI: 10.3390/jfb11020036. PMID: 32492906
- Bossù, M.; Saccucci, M.; Salucci, A.; Giorgio, D.G.; Bruni, E.; Uccelletti, D.; Sarto, M.S.; Familiari, G.; Relucenti, M.; Polimeni, A. Enamel remineralization and repair results of Biomimetic Hydroxyapatite toothpaste on deciduous teeth: an effective option to fluoride toothpaste. J Nanobiotechnology. 2019, 17, 17. DOI: 10.1186/s12951-019-0454-6. PMID: 30683113
- Lelli, M.; Putignano, A.; Marchetti, M.; Foltran, I.; Mangani, F.; Procaccini, M.; Roveri, N.; Orsini, G. Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing toothpaste: a comparative in vivo study. Front Physiol. 2014, 5, 333. DOI: 10.3389/fphys.2014.00333. PMID: 25249980
Author Response File: Author Response.pdf
