Effect of Genotype and Sex on Chemical Composition, Physicochemical Properties, Texture and Microstructure of Spent Broiler Breeder Meat
Round 1
Reviewer 1 Report
The manuscript compared the carcass composition and meat quality characteristics of 2 broiler breeders considering effect of sex, which provided detailed data for introducing spent laying hens into the poultry meat supply chain. The linguistic articulation of this manuscript is notably coherent. However, the experimental design appears relatively straightforward, and further refinement is also recommended in the discourse of the results and discussions. There are some suggestions and errors as fellows:
1. In section of Introduction, the background of Cobb 500 and Ross 308 broiler breeders was not elaborated, and the difference of breeding objectives and production characteristics of the 2 stains were suggested to describe. And these are important for explaining the purpose and significance of choosing the 2 breeds.
2. In Materials and Methods section, the 2 breeds were purchased from a commercial slaughterhouse, could the origin of these 2 species can be traced back, whether they come from the same farm, the age and raising condition are the same? These are vital factors for the experimental design and result.
3. In table 1 to table 6, “*” was used to display statistical differences between males and females, and I have not seen this description style, please recheck if this is appropriated.
4. In line 178、208 et al., carcass composition and meat quality indicators in the current study were used to compared with previous study. Since ages, raising condition (environmental factors) and other potential factor are not consist, I do not think this would be objective and persuasive. Besides, in line 177-178, the difference in “Cobb 500” and “Hubbard Hi-Y” and “Ross 508”should not be described as “significantly higher”, since t-test and multiple comparisons were not performed.
Author Response
Reviewer 1
General comments:
The manuscript compared the carcass composition and meat quality characteristics of 2 broiler breeders considering effect of sex, which provided detailed data for introducing spent laying hens into the poultry meat supply chain. The linguistic articulation of this manuscript is notably coherent. However, the experimental design appears relatively straightforward, and further refinement is also recommended in the discourse of the results and discussions.
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Comments |
Answers |
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In section of Introduction, the background of Cobb 500 and Ross 308 broiler breeders was not elaborated, and the difference of breeding objectives and production characteristics of the 2 stains were suggested to describe. And these are important for explaining the purpose and significance of choosing the 2 breeds. |
As suggested by the Reviewer, a fragment of the text has been added – “Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle”
„. Ross was started in Scotland in Edinburgh when Chunky Chicks Limited opened and then Aviagen continued its genetic development and global expansion which has led to Ross being present in over 100 countries on six continents [5]. The Cobb breed, on the other hand, was selected and developed in England in the 1970s. Because retailers were looking for a product that would look good on fresh meat shelves. The Cobb 500 broiler is a modern commercial breed with rapid initial growth and high yields of breast meat at different processing ages compared to other commercial varieties currently grown worldwide |
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In Materials and Methods section, the 2 breeds were purchased from a commercial slaughterhouse, could the origin of these 2 species can be traced back, whether they come from the same farm, the age and raising condition are the same? These are vital factors for the experimental design and result. |
The carcasses of the birds obtained for the study were of the same age, 61 weeks - Line 98. During the reproductive period, they were fed complete feeds for parent flocks, according to the instructions for keeping the flock Ross 308 and Cobb 500 in regulated environmental conditions. |
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In table 1 to table 6, “*” was used to display statistical differences between males and females, and I have not seen this description style, please recheck if this is appropriated. |
In other works, the results were also presented in this way, for example: Kokoszyński, D. et al. 2016. Comparison of carcass composition, physicochemical and sensory traits of meat from spent broiler breeders with broilers. Europ. Poult. Sci., 80, 1-11.
Kokoszyński, D. et al. 2022. Effect of genotype and sex on carcass composition, physicochemical properties, texture and microstructure of meat from geese after four reproductive seasons. British Poultry Science, 63, 519-527. |
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In line 178、208 et al., carcass composition and meat quality indicators in the current study were used to compared with previous study. Since ages, raising condition (environmental factors) and other potential factor are not consist, I do not think this would be objective and persuasive. |
The parent flocks are kept in poultry houses with regulated environmental conditions and are managed in accordance with the Ross 308 parent flock management instructions. They are also fed with complete feed intended for reproductive flocks of a specific composition. Carcasses of birds of different ages were compared. |
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Besides, in line 177-178, the difference in “Cobb 500” and “Hubbard Hi-Y” and “Ross 508”should not be described as “significantly higher”, since t-test and multiple comparisons were not performed. |
As suggested by the Reviewer, “significantly higher” was removed and replaced with “higher” |
Author Response File: 
Author Response.docx
Reviewer 2 Report
Title:
Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat
General comments:
The aim of this study, “Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat” is not clear. Different aims are described in the different sections of the manuscripts. Firstly, the authors want to enhance the utilization of the spent breeders in the form of processed products. Then I think this study should be done on the processed products of the spent breeder rather than on raw thigh and breast meat. Secondly, what were the reasons to compare Ross and Cobb breeders? As the authors indicated over 85% of broiler breeder flocks maintained in Poland consist of Ross 308 breeders, then I think it was more convenient to explore the processing characteristics of Ross 308 only.
Abstract:
Line25: Replace cooking loss and lightness with “cooking loss and lightness values”.
Introduction:
Lines 48-49: What were the reasons to compare Ross and Cobb breeders? As the authors indicated over 85% of broiler breeder flocks maintained in Poland consist of Ross 308 breeders, then I think it was more convenient to explore the processing characteristics of Ross 308 only. Justify
Line 59: Replace “Aviagen and Cobb-Vantress” with “Aviagen and Cobb-Vantress, respectively”.
Materials and methods:
Line 102: Mention the specifications of the scale
Line 112: Maintain the uniformity, initial the authors mentioned the breast muscle and thigh muscle and now here at line 112, they replace the thigh muscle with drumstick muscle.
Line 119: Provide the reference of cooking time and temperature.
Line 135: How the temperature of the muscles was measured?
Results and discussion:
Provide the statistically significant differences between two muscles.
Describe the results and discussions of cooking loss and EC24 before the color parameters.
Conclusion:
The conclusion section is not depicting the findings of the study; therefore, revise it according to the findings of the study.
Minor editing of English language required
Author Response
Reviewer 2
General comments:
The aim of this study, “Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat” is not clear. Different aims are described in the different sections of the manuscripts.
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Comments |
Answers |
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Firstly, the authors want to enhance the utilization of the spent breeders in the form of processed products. Then I think this study should be done on the processed products of the spent breeder rather than on raw thigh and breast meat. |
The results presented in this manuscript are only part of research conducted with other research centers in Poland. The intention of the authors of this work was to show the differences in quality between the raw material obtained from different genetic lines. The authors also have results obtained during other experiments in which this meat was used for the production of, among others, cooked sausages. These results will be published after they are supplemented with additional analyzes and statistical analysis. |
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Secondly, what were the reasons to compare Ross and Cobb breeders? As the authors indicated over 85% of broiler breeder flocks maintained in Poland consist of Ross 308 breeders, then I think it was more convenient to explore the processing characteristics of Ross 308 only. |
In Poland, as indicated, approximately 85% of Ross 308 parent herds are kept, while in the world the difference between the two genotypes is much smaller. Therefore, these two sets of hybrids were selected for research. |
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Line25: Replace cooking loss and lightness with “cooking loss and lightness values”. |
Changed in accordance with the Reviewer's recommendation |
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Lines 48-49: What were the reasons to compare Ross and Cobb breeders? As the authors indicated over 85% of broiler breeder flocks maintained in Poland consist of Ross 308 breeders, then I think it was more convenient to explore the processing characteristics of Ross 308 only. Justify |
Two sets, Ross 308 and Cobb 500, were selected for research because they are the most popular sets used in the world. In Poland, Ross 308 has a much larger market share, while in the world the difference between these sets is much smaller. The conducted research provides information to the processing industry not only in Poland but also in the world. That is why we focused not only on Ross 308 hybrids, but also on Cobb 500, whose population is definitely smaller in Poland and oscillates around 5-10%. |
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Line 59: Replace “Aviagen and Cobb-Vantress” with “Aviagen and Cobb-Vantress, respectively”.
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It has been changed as recommended |
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Line 102: Mention the specifications of the scale
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As suggested, the text was added: “weighed on an electronic scale with accuracy to 1 g” |
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Line 112: Maintain the uniformity, initial the authors mentioned the breast muscle and thigh muscle and now here at line 112, they replace the thigh muscle with drumstick muscle. |
Changed to “the thigh muscle” |
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Line 119: Provide the reference of cooking time and temperature. |
Walczak, Z. (1959). Laboratoryjna metoda oznaczania zawartości galarety w konserwach mięsnych [Laboratory method for determining the content of jelly in canned meat]. Rocz. Nauk. Rol., 74-B-4, 619–621 [in Polish]. |
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Line 135: How the temperature of the muscles was measured? |
The breast and thigh muscle samples were placed separately in thermoresistant plastic bags, cooked in water at 85°C until the geometric center reached 68°C, and also cooled in cold water in the same bag. The temperature was controlled using a thermometer DT34 KERA (TERMOPRODUKT, Poland) inserted into the sample with the smallest and largest mass (separately for the breast and thigh muscles). The time needed to cook the breast muscles was about 10 minutes, and the thigh muscles 7-8 minutes. This text fragment has been corrected by removing unnecessary information about the cooking time. |
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Results and discussion: Provide the statistically significant differences between two muscles. Describe the results and discussions of cooking loss and EC24 before the color parameters. |
It was changed at manuscript as recommended. |
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Conclusion: The conclusion section is not depicting the findings of the study; therefore, revise it according to the findings of the study. |
This part of the text has been edited. |
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
1.Since Cobb 500 and Ross 308 are all commercial lines, and the excellent production performance comes from the hybrid advantage of terminal matriarchal and patriarchal broiler. And materials in the current study of spent broiler breeders actually refers the terminal matriarchal and patriarchal broiler, of which production performance characteristics are usually different with commercial lines. So the background of spent broiler breeder should be described.
2.”Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle” The expression of sentences is confusing, “4.3 percent higher” compared with which breed?
Author Response
Bydgoszcz, September 06, 2023
Dear Editor,
Enclosed please find our response to Reviewer’s comments on article: agriculture-2581132_R1 entitled ‘Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat” written by Marcin Wegner, Dariusz Kokoszyński, Joanna Żochowska-Kujawska, Marek Kotowicz.
All comments of both Reviewers have been included in the revised text. The authors would like to thank for all valuable comments that may contribute to improving the quality of this manuscript.
Sincerely yours,
Marcin Wegner
Dariusz Kokoszyński
Joanna Żochowska-Kujawska
Marek Kotowicz
Reviewer 1 comments and responses to comments
Open Review
( ) I would not like to sign my review report
(x) I would like to sign my review report
Quality of English Language
( ) I am not qualified to assess the quality of English in this paper
( ) English very difficult to understand/incomprehensible
( ) Extensive editing of English language required
( ) Moderate editing of English language required
( ) Minor editing of English language required
(x) English language fine. No issues detected
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Yes |
Can be improved |
Must be improved |
Not applicable |
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Does the introduction provide sufficient background and include all relevant references? |
(x) |
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Are all the cited references relevant to the research? |
(x) |
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( ) |
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Is the research design appropriate? |
(x) |
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( ) |
( ) |
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Are the methods adequately described? |
(x) |
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( ) |
( ) |
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Are the results clearly presented? |
(x) |
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( ) |
( ) |
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Are the conclusions supported by the results? |
(x) |
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Comments and Suggestions for Authors
Comment_1
1.Since Cobb 500 and Ross 308 are all commercial lines, and the excellent production performance comes from the hybrid advantage of terminal matriarchal and patriarchal broiler. And materials in the current study of spent broiler breeders actually refers the terminal matriarchal and patriarchal broiler, of which production performance characteristics are usually different with commercial lines. So the background of spent broiler breeder should be described.
Answer:
The origin of the compared broiler breeder flocks is described in lines 63-68, and their reproductive performance in lines 53-55. The authors of the manuscript do not have data on the name and performance of maternal or paternal lines selected on breeding farms, which are used to produce hybrid roosters and hens kept on parent farms. They are subject to trade secrets.
Text added in the Materials and Methods chapter
L112-115
According to the contracting department of the slaughterhouse during the reproductive period, they were kept on the same farm and fed complete feeds for parent flocks, according to the instructions for managing the flock Ross 308 and Cobb 500 in regulated environmental conditions.
Comment 2
”Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle” The expression of sentences is confusing, “4.3 percent higher” compared with which breed?
Answer
Sentence changed to:
Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs than Cobb 500 broiler breeder with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle [3].
Submission Date
11 August 2023
Date of this review
05 Sep 2023 04:08:21
Reviewer 2 comments and responses to comments
( ) I would not like to sign my review report
(x) I would like to sign my review report
Quality of English Language
( ) I am not qualified to assess the quality of English in this paper
( ) English very difficult to understand/incomprehensible
( ) Extensive editing of English language required
( ) Moderate editing of English language required
( ) Minor editing of English language required
(x) English language fine. No issues detected
Początek formularza
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Yes |
Can be improved |
Must be improved |
Not applicable |
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Does the introduction provide sufficient background and include all relevant references? |
(x) |
( ) |
( ) |
( ) |
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Are all the cited references relevant to the research? |
( ) |
(x) |
( ) |
( ) |
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Is the research design appropriate? |
(x) |
( ) |
( ) |
( ) |
|
Are the methods adequately described? |
(x) |
( ) |
( ) |
( ) |
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Are the results clearly presented? |
( ) |
(x) |
( ) |
( ) |
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Are the conclusions supported by the results? |
(x) |
( ) |
( ) |
( )
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Comments and Suggestions for AuthorsDół formularza
Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat
Comment 1
Provide the reference of cooking time and temperature at the appropriate place i.e. after geometric centre reached 68°C.
Answer
until the geometric center reached 68°C changed to "for 10 minutes" according to the methodology.
Comment 2
The revised version has not provided statistically significant differences between the two muscles.
Answer
Statistically significant differences are marked in tables 2-4. Text added to the Abstract chapter.
L35-38
Regardless of the origin and sex, significant differences were found between the breast and leg muscles in terms of the assessed physicochemical features (pH24, EC24, cooking loss), color attributes (Lab) and chemical composition (protein, intramuscular fat, and collagen contents).
Comment 3
Mention the statistical difference for BM and LM muscles by applying the superscripts in columns in Tables 2, 3, and 4.
Answer
Statistically significant differences were marked as superscript "x" in Tables 2-4.
Author Response File: Author Response.docx
Reviewer 2 Report
Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat
General comments:
Provide the reference of cooking time and temperature at the appropriate place i.e. after geometric centre reached 68°C.
The revised version has not provided statistically significant differences between the two muscles.
Mention the statistical difference for BM and LM muscles by applying the superscripts in columns in Tables 2, 3, and 4.
Author Response
Bydgoszcz, September 06, 2023
Dear Editor,
Enclosed please find our response to Reviewer’s comments on article: agriculture-2581132_R1 entitled ‘Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat” written by Marcin Wegner, Dariusz Kokoszyński, Joanna Żochowska-Kujawska, Marek Kotowicz.
All comments of both Reviewers have been included in the revised text. The authors would like to thank for all valuable comments that may contribute to improving the quality of this manuscript.
Sincerely yours,
Marcin Wegner
Dariusz Kokoszyński
Joanna Żochowska-Kujawska
Marek Kotowicz
Reviewer 1 comments and responses to comments
Open Review
( ) I would not like to sign my review report
(x) I would like to sign my review report
Quality of English Language
( ) I am not qualified to assess the quality of English in this paper
( ) English very difficult to understand/incomprehensible
( ) Extensive editing of English language required
( ) Moderate editing of English language required
( ) Minor editing of English language required
(x) English language fine. No issues detected
|
Yes |
Can be improved |
Must be improved |
Not applicable |
|
|
Does the introduction provide sufficient background and include all relevant references? |
(x) |
( ) |
( ) |
( ) |
|
Are all the cited references relevant to the research? |
(x) |
( ) |
( ) |
( ) |
|
Is the research design appropriate? |
(x) |
( ) |
( ) |
( ) |
|
Are the methods adequately described? |
(x) |
( ) |
( ) |
( ) |
|
Are the results clearly presented? |
(x) |
( ) |
( ) |
( ) |
|
Are the conclusions supported by the results? |
(x) |
( ) |
( ) |
( ) |
Comments and Suggestions for Authors
Comment_1
1.Since Cobb 500 and Ross 308 are all commercial lines, and the excellent production performance comes from the hybrid advantage of terminal matriarchal and patriarchal broiler. And materials in the current study of spent broiler breeders actually refers the terminal matriarchal and patriarchal broiler, of which production performance characteristics are usually different with commercial lines. So the background of spent broiler breeder should be described.
Answer:
The origin of the compared broiler breeder flocks is described in lines 63-68, and their reproductive performance in lines 53-55. The authors of the manuscript do not have data on the name and performance of maternal or paternal lines selected on breeding farms, which are used to produce hybrid roosters and hens kept on parent farms. They are subject to trade secrets.
Text added in the Materials and Methods chapter
L112-115
According to the contracting department of the slaughterhouse during the reproductive period, they were kept on the same farm and fed complete feeds for parent flocks, according to the instructions for managing the flock Ross 308 and Cobb 500 in regulated environmental conditions.
Comment 2
”Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle” The expression of sentences is confusing, “4.3 percent higher” compared with which breed?
Answer
Sentence changed to:
Broiler breeder Ross 308 was characterized by a 4.3 percent higher laying capacity and 6 more hatching eggs than Cobb 500 broiler breeder with an average fertilization rate of 90.5% were obtained from one hen in the entire reproductive cycle [3].
Submission Date
11 August 2023
Date of this review
05 Sep 2023 04:08:21
Reviewer 2 comments and responses to comments
( ) I would not like to sign my review report
(x) I would like to sign my review report
Quality of English Language
( ) I am not qualified to assess the quality of English in this paper
( ) English very difficult to understand/incomprehensible
( ) Extensive editing of English language required
( ) Moderate editing of English language required
( ) Minor editing of English language required
(x) English language fine. No issues detected
Początek formularza
|
Yes |
Can be improved |
Must be improved |
Not applicable |
|
|
Does the introduction provide sufficient background and include all relevant references? |
(x) |
( ) |
( ) |
( ) |
|
Are all the cited references relevant to the research? |
( ) |
(x) |
( ) |
( ) |
|
Is the research design appropriate? |
(x) |
( ) |
( ) |
( ) |
|
Are the methods adequately described? |
(x) |
( ) |
( ) |
( ) |
|
Are the results clearly presented? |
( ) |
(x) |
( ) |
( ) |
|
Are the conclusions supported by the results? |
(x) |
( ) |
( ) |
( )
|
Comments and Suggestions for AuthorsDół formularza
Effect of genotype and sex on chemical composition, physicochemical properties, texture and microstructure of spent broiler breeder meat
Comment 1
Provide the reference of cooking time and temperature at the appropriate place i.e. after geometric centre reached 68°C.
Answer
until the geometric center reached 68°C changed to "for 10 minutes" according to the methodology.
Comment 2
The revised version has not provided statistically significant differences between the two muscles.
Answer
Statistically significant differences are marked in tables 2-4. Text added to the Abstract chapter.
L35-38
Regardless of the origin and sex, significant differences were found between the breast and leg muscles in terms of the assessed physicochemical features (pH24, EC24, cooking loss), color attributes (Lab) and chemical composition (protein, intramuscular fat, and collagen contents).
Comment 3
Mention the statistical difference for BM and LM muscles by applying the superscripts in columns in Tables 2, 3, and 4.
Answer
Statistically significant differences were marked as superscript "x" in Tables 2-4.
Author Response File: Author Response.docx
