Leelamine Modulates STAT5 Pathway Causing Both Autophagy and Apoptosis in Chronic Myelogenous Leukemia Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Lines and Culture Conditions
2.3. MTT Assay
2.4. Western Blot Analysis
2.5. RT-PCR Analysis
2.6. Live/Dead Assay
2.7. Acridine Orange Assay
2.8. MDC Staining
2.9. TUNEL Assay
2.10. Immunocytochemistry for LC3 Expression
2.11. Knockdown of Beclin-1 and Atg7 Expression by siRNA Transfection
2.12. Blockage of STAT5 Expression by siRNA
2.13. Bcl-2 Overexpression by pEGFP Bcl-2 Transfection
2.14. Statistical Analysis
3. Results
3.1. LEE-Promoted Cell Death through Causing Apoptosis
3.2. LEE-Triggered Autophagy via Production of Acidic Vesicular Organelles
3.3. Modulation of Various Cell Survival Proteins by LEE
3.4. LEE-Induced Cell Death through Autophagy and Apoptosis Even in the Presence of Different Pharmacological Inhibitors
3.5. LEE-Induced Autophagy and Apoptosis via Driving STAT5 Inhibition
3.6. Overexpression of bcl-2 Reduced Autophagy and Apoptosis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jung, Y.Y.; Um, J.-Y.; Chinnathambi, A.; Govindasamy, C.; Sethi, G.; Ahn, K.S. Leelamine Modulates STAT5 Pathway Causing Both Autophagy and Apoptosis in Chronic Myelogenous Leukemia Cells. Biology 2022, 11, 366. https://doi.org/10.3390/biology11030366
Jung YY, Um J-Y, Chinnathambi A, Govindasamy C, Sethi G, Ahn KS. Leelamine Modulates STAT5 Pathway Causing Both Autophagy and Apoptosis in Chronic Myelogenous Leukemia Cells. Biology. 2022; 11(3):366. https://doi.org/10.3390/biology11030366
Chicago/Turabian StyleJung, Young Yun, Jae-Young Um, Arunachalam Chinnathambi, Chandramohan Govindasamy, Gautam Sethi, and Kwang Seok Ahn. 2022. "Leelamine Modulates STAT5 Pathway Causing Both Autophagy and Apoptosis in Chronic Myelogenous Leukemia Cells" Biology 11, no. 3: 366. https://doi.org/10.3390/biology11030366
APA StyleJung, Y. Y., Um, J. -Y., Chinnathambi, A., Govindasamy, C., Sethi, G., & Ahn, K. S. (2022). Leelamine Modulates STAT5 Pathway Causing Both Autophagy and Apoptosis in Chronic Myelogenous Leukemia Cells. Biology, 11(3), 366. https://doi.org/10.3390/biology11030366