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Review

Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient

by
Iris Catiana Zampini
1,2,
Ana Lia Salas
1,2,
Luis M. Maldonado
3,
Mario J. Simirgiotis
4 and
María Inés Isla
1,2,*
1
Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV, CONICET-UNT), San Miguel de Tucumán 4000, Argentina
2
Facultad de Ciencias Naturales, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán 4000, Argentina
3
Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Famaillá, Ruta Provincial 301–km 32, Famaillá, San Miguel de Tucumán 4000, Argentina
4
Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5090000, Chile
*
Author to whom correspondence should be addressed.
Metabolites 2021, 11(2), 76; https://doi.org/10.3390/metabo11020076
Submission received: 15 December 2020 / Revised: 22 January 2021 / Accepted: 22 January 2021 / Published: 28 January 2021
(This article belongs to the Special Issue Plant and Derived Natural Product Metabolomics)
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Abstract

:
The aim of this review is to provide overall information on Argentine propolis and to shed light on its potential, especially the one from the Monte region so as to support future research in the field. Around 1999, the Argentine propolis began to be chemically and functionally characterized to give it greater added value. Because Argentina has a wide plant biodiversity, it is expected that its propolis will have various botanical origins, and consequently, a different chemical composition. To date, five types have been defined. Based on their functionality, several products have been developed for use in human and veterinary medicine and in animal and human food. Because the Argentine propolis with the greatest potential is that of the Monte eco-region, this review will describe the findings of the last 20 years on this propolis, its botanical source (Zuccagnia punctata Cav.), its chemical composition, and a description of markers of chemical quality (chalcones) and functionality. Propolis can regulate the activity of various pro-inflammatory enzymes and carbohydrate and lipid metabolism enzymes, as well as remove reactive oxygen and nitrogen species. Consequently, it can modulate metabolic syndrome and could be used as a functional ingredient in food. Furthermore, hydroalcoholic extracts can act against human and animal pathogenic bacteria and human yeast, and mycelial pathogenic fungi. The ability to stop the growth of post-harvest pathogenic bacteria and fungi was also demonstrated. For this reason, Argentine propolis are natural products capable of protecting crops and increasing the lifespan of harvested fruit and vegetables. Several reports indicate the potential of Argentine propolis to be used in innovative products to improve health, food preservation, and packaging. However, there is still much to learn about these natural products to make a wholesome use of them.

Graphical Abstract

1. Introduction

Propolis (bee glue) is a natural product produced by Apis mellifera from resins collected from different parts of plants, namely, buds, young leaves, stems, and cracks in the bark, by mixing with wax and saliva. Bees use it to block cracks and to cover the internal walls of the hive as a defense system against microbial infections, parasites, and insects [1].
Propolis was widely used as phytomedicine for its anti-inflammatory, immunomodulatory, antioxidant, antimicrobial, antiparasitic, antiviral, antiaging, anesthetic, cytotoxic, antitumoral, hypolipidemic, and hypoglycemic activity, among others [2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20]. The antimicrobial and antioxidant properties are valuable in the food industry because of its positive effect on food-product stability and shelf life. Propolis has a potential as a natural food additive and functional food ingredient [21,22,23,24,25,26]. By the beginning of this century, Marcucci [3] and Bankova et al. [27] reported more than 300 constituents in propolis, and in the last twenty years, at least 550 new compounds have been isolated from it [28]. The vegetation around the hives and the preference of honeybees toward specific available botanical sources, determines its chemical diversity; hence, different propolis types exist. In a given geographic region, bees usually show preference for one or two plants. Although it is clear that they select specific sources, the cues used for finding a resin source are virtually unknown [1]. Some studies suggest common plant sources and similar chemical profiles for large geographical areas. Propolis from temperate regions in North America, Europe, and non-tropical regions of Asia derive from poplars (Populus spp.) and birches (Betula spp.) [27,28,29,30,31]. Clusia minor was described as the plant source of Venezuela propolis [32]. A brown propolis from Clusia rosea resin and a red propolis from Dalbergia spp. were described in Cuba [33]. The most popular propolis from Brazil are both green or alecrim propolis, originating from Baccharis dracunculifolia, and red propolis, originating from Dalbergia ecastaphyllum. Propolis of Brazil also come from Hyptis divaricata and Populus nigra [29]. According to Koenig (1995) [34] and Montenegro et al. (2001) [35], the most frequent botanical sources of propolis from central Chile are Salix humboldtiana and Eucalyptus globulus.
The aim of this review is to provide overall information on Argentine propolis and to shed light on their potential, placing special emphasis on propolis from the Monte region in order to both promote their use and support future research.

2. Research on Argentine Propolis

Beekeeping is one of the main activities in Argentine economy. Around 1999, the Argentine propolis began to be chemically and functionally characterized to enhance its value. Because Argentina has a wide plant biodiversity with several phytogeographical regions (Monte region, Gran Chaco region, Puna and Prepuna regions, and Yunga region), it is expected that its propolis will have various botanical origins and, consequently, a different chemical composition according to the place where the hives are placed. Because of the chemical variability of propolis, the study was not limited to a single specimen in each area. In addition, the studies on biological activities were also performed by using different experimental models and samples from different years. The propolis was classified according to collection sites in (a) propolis from Northwestern Argentina, (b) propolis from northeastern Argentina, (c) propolis from Cuyo or Andean region, (d) propolis from the central region, and (e) propolis from southern Argentina [36]. The chemical composition of northwestern Argentine propolis (Provinces of Tucumán, Santiago del Estero, Salta, Chaco, and Catamarca) was reported for the first time in 2005 [37]. Since then, more than 60 chemical components in propolis from this region have been identified [37,38,39,40,41,42,43,44,45,46,47,48], with the propolis from the Monte region being the most widely studied. Thirteen propolis components from the northeast (Provinces of Chaco and Misiones) were recorded [37]; whereas around eleven components were detected in the Cuyo region (Provinces of San Juan and Mendoza) [49,50,51,52]; five were identified in the central region, in Santa Fe [53]; ten phenolic compounds in propolis from Buenos Aires [54]; three in La Pampa; and thirteen in Entre Rios [52], Table 1. The chemical components of propolis from the south of the country were also studied, specifically those from Rio Negro, where eleven components were found [52], Table 1. Various propolis types have also been reported in Argentina, depending on their botanical origin. The species Larrea nitida and Baccharis are sources of propolis from the Andean region [55,56] and Zuccagnia punctata is a source of propolis from the Monte ecoregion in northwestern Argentina [38,40,44,45,46,47,48,57,58,59,60]. The Argentine propolis has several functional properties such as antibacterial, antifungal, anti-inflammatory, antioxidant, nematicidic, and cytotoxic, among others, apart from being an inhibitor of enzymes linked to metabolic syndrome [36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,61,62,63,64]. Based on their properties, several products have been developed to date for use in human and veterinary medicine and in the food industry. Furthermore, Argentina has made progress in terms of quality control regulations of crude propolis and propolis extracts (IRAM-INTA15935-1 and -2 normative), and the propolis was included in the Argentine Food Code as a dietary supplement in May 2008 [26].

2.1. Propolis from the Monte Region in Argentina

The Monte ecoregion is exclusive to Argentina. It extends from the province of Jujuy (Quebrada de Humahuaca) to the northeast of Chubut. The Monte region in northern Argentina is a temperate and arid zone, where the predominant vegetation is xerophytic and halophytic shrub-steppe. The climax community of the Monte is the “jarillal”, an association of Zuccagnia punctata, Larrea divaricata, and Larrea cuneifolia (Figure 1).

2.1.1. Chemical Characterization of Propolis from the Monte Region

Phenolic acid, flavone, flavanone, and chalcones were isolated and identified in propolis from the Monte region by using different technologies (Table 2). H NMR and UV spectra were used to isolate and identify 12 phenolic compounds in propolis from Catamarca in the Monte region, namely, flavanone (7-hydroxy-8-methoxyflavanone; 7,4′-dihydroxy-5-methoxyflavanone; 3β, 7-dihydroxy-5-methoxyflavanone; 7-dihydroxy-5,8-dimethoxyflavanone), flavones (4′, 5-dihydroxy-3,7,8-trimethoxyflavone; 5-hydroxy-4′,7-dimethoxyflavone; 3,7-dihydroxy-8-methoxyflavone; 3,5-dihydroxy-7,8-dimethoxyflavone; 7-hydroxy-5,8 dimethoxyflavone), and chalcones (2′,4′-dihydroxychalcone; 2′,4′-dihydroxy-3′-methoxychalcone; 2′,4′,4-trihydroxy-6′-methoxychalcone) [41]. UV-spectrum, mass spectra and fragmentation patterns were used to identify nine compounds in propolis samples from another site in Catamarca in the Monte region, namely, flavanone (3,5,7-trihydroxyflavanone (pinobanksin); 7-hydroxyflavanone; 5-hydroxy 7-methoxyflavanone; 7-hydroxy 8-methoxyflavanone), flavone (3,7-dihydroxy 8-methoxyflavone; 5,7-dihydroxyflavona (Chrysin); 3,5-dihydroxy 7,8-dimethoxy-flavone), and chalcones (2′,4′-dihydroxychalcone and 2′,4′-dihydroxy-3′-methoxychalcone) [45]. Various phenolic compounds were identified in propolis from the Monte region in Tucuman, namely, phenolic acids and their ester (cinnamic acid; caffeic acid prenyl ester; caffeoyl dihydrocaffeate; 3,4-dihydroxy-β-phenylethyl caffeate or teucrol; 1-methyl-3-(4-hydroxyphenyl)-propyl caffeic acid; 1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl caffeic acid ester; 1-methyl-3-(4 -hydroxyphenyl)-propyl p-coumaric acid ester; 4′-terbutyloxyphenyl p-coumaric acid ester; 1-methyl-3-(4′-hydroxyphenyl)-propyl p-coumaric acid ester; 3,7-dimethyl-2,6-octadienyl caffeic acid ester (geranyl caffeate); 1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl ferulic acid ester; 2-methyl-3-(3′-hydroxy-4′-methoxyphenyl)-propyl caffeic acid ester; flavanones (7-hydroxyflavanone and 7-hydroxy-8-methoxyflavanone; 7,8-dihydroxyflavanone; 3,7-dihydroxyflavanone; pinobanksin-5-methyl ether (3,7-dihydroxy-5-methoxyflavanone); 4′,7-dihydroxyflavanone (liquiritigenin); 5,7-dihydroxyflavanone (pinocembrin); 5-hydroxy-7-methoxyflavanone (pinostrobin)); flavones such as 7-O-methylgalangin (izalpinin); 3,5,7 trihydroxyflavone (galangin); 3,4′,5-trihydroxy-7-methoxyflavone (rhamnocitrin); and 3-hydroxy-7,8-dimethoxyflavone; and two chalcones named as 2′,4′-dihydroxychalcone and 2′,4′-dihydroxy-3′-methoxychalcone [40,44,46,47]. The two latter compounds were identified for the first time in Argentine propolis, and they were considered as chemical markers of propolis samples previously analyzed from the Monte region [38,40,44]. Then, two dihydrochalcones (4′-hydroxy-2′-methoxydihydrochalcone and 2′,4′-dihydroxydihydrochalcone) were also identified [47]. Chalcones are not very common compounds in propolis in other parts of the world. They were only identified in red propolis obtained from hives in the northern region of Brazil [65] and in propolis from apiaries located in the central southern region of Kangaroo Island [66].
Solorzano et al. (2019) [47] reported also minor compounds such as geranyl, pentenyl, and benzyl caffeate and cinnamyl caffeate. The major volatile compounds reported in Zuccagnia-type propolis was trans-linalool oxide (furanoid), cis-linalool oxide (furanoid), linalool, chrysanthenone, p-cymen-8-ol, and 2,3,6-trimethylbenzaldehyde p-mentha-1,5-dien-8-ol, (E)-anethole, α-terpineol, and cis-linalyl oxide (pyranoid) [48].

2.1.2. Botanical Origin by Microscopic Analyses and Chemical Analysis

Determining the plant source is most important as it can help beekeepers select the place to place the hives so as to increase the production of propolis and achieve its standardization. The botanical source of propolis from the Monte region was determined by two methods, one by microscopic analysis of a propolis sample in order to identify fragments of leaves [57,58] of plant species that grow in this region. Z. punctata, L. divaricata and L. cuneifolia are the most abundant species in the Monte region (Figure 1A). The former was identified by the presence of compound leaf primordia with sub-opposite nanophyll leaflets with acuminate apex, rounded, symmetrical base and entire margin, free leaflet fragments with spherical to oval sunken capitate multicellular glandular trichomes and unicellular non-glandular trichomes arranged on the adaxial base of the foliar surface and on the foliar margins, leaflet epidermal cells with straight anticlinal walls, cyclocytic stoma, rarely paracytic or anomocytic on both epidermal surfaces (Figure 1B). These features respond to those described for Z. punctata in previous work [59,60]. Microscopic analyses of propolis samples revealed the presence of Z. punctata leaf as a major plant species.
Although Larrea leaves [56] were not found, pollen of different plants from the region was found, including pollen from Zuccagnia and Larrea [58]. To confirm the botanical origin of propolis samples coming from the ecoregion of Monte of Sierras and Bolsones, a chemical characterization by HPLC-DAD of resin extracts from three jarilla species (Z. punctata, L. divaricata, and L. cuneifolia) was carried out and compared with propolis extracts [40,57,58]. Major chemical components of the propolis and Z. punctata resin were 2′,4′-dihydroxychalcone (DHC) and 2′,4′-dihydroxy-3′-methoxychalcone (DHMC) and were considered as chemical markers of Zuccagnia-type propolis samples [44]. Chemical components, such as nordihydroguaiaretic acid, the major chemical compound of both Larrea species, were not found in the propolis extracts.
According to the chemical results, the botanical origin of propolis samples from the Monte region could be Z. punctata. Then, by using a liquid chromatography–diode array detector–quadrupole time-of-flight system (LC-DAD-QTOF), Solorzano et al. (2017) [46] identified some chemical components of the Monte region propolis and Z. punctata extracts, such as flavanones (7-hydroxyflavanone (HF) and 7-hydroxy-8-methoxyflavanone; 7,8-dihydroxyflavanone; 3,7-dihydroxyflavanone (DHF); pinobanksin-5-methyl ether (3,7-dihydroxy-5-methoxyflavanone); 3,7,8-trihydroxy dihydroflavanone); chalcones (2′,4′-dihydroxychalcone and 2′,4′-dihydroxy-3′-methoxychalcone), which were previously reported; and two new dihydrochalcones (4′-hydroxy-2′-methoxy dihydrochalcone and 2′,4′-dihydroxydihydrochalcone) and phenolic acid and esters: 1-methyl-3-(4-hydroxyphenyl)-propyl caffeic acid; 1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl caffeic acid ester; 1-methyl-3-(4-hydroxyphenyl)-propyl p-coumaric acid ester; 4′-terbutyloxyphenyl p-coumaric acid ester; 1-methyl-3-(4′-hydroxyphenyl)-propyl p-coumaric acid ester; 3,7-dimethyl-2,6-octadienyl caffeic acid ester (geranyl caffeate); 1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl ferulic acid ester; 2-methyl-3-(3′-hydroxy-4′-methoxyphenyl)-propyl caffeic acid ester, see Table 2.

2.1.3. Pharmacological Activities

Argentine propolis from the Monte ecoregion has multiple pharmacological properties (Figure 2), which have been reported in the last 20 years. These properties have made significant progress.

2.1.3.1. Antibacterial Activity

The prevalence of multidrug resistant bacteria versus commercial antibiotics has left healthcare systems with few treatment options, which are generally expensive therapies. In recent years, alternative and more specific antimicrobials that complement conventional therapy have been studied. Phenolic compounds of plant origin exhibit antibacterial activity by mechanisms different from conventional drugs, thus rendering bacteria unable to develop resistance [67]. Propolis from hives in the Monte region showed antibacterial activity against antibiotic resistant Gram-positive and Gram-negative bacteria isolated from skin and soft tissue infections [41,43,44,45,68]. These propolis showed greater activity against Gram-positive bacteria than against Gram-negative bacteria with minimal inhibitory concentration (MIC) values between 10 and 100 µg/mL and between 400 and 1600 µg/mL, respectively.
The propolis also showed antibacterial activity against microorganism isolates from canine otitis such as Staphylococcus haemolyticus, S. aureus, and S. intermedius with MIC values between 75 and 150 μg/mL, and minimal bactericidal concentration (MBC) values between of 200 and 600 μg/mL. According to the results of the in situ bioautographic tests, DHC and DHMC would be responsible for the inhibition of the growth of Gram-positive bacteria, principally Staphylococcus aureus isolated from human and animal infections [43,45,62].
The antibacterial activity exerted by the propolis extracts against common human and animal pathogenic strains suggests their potential application in the treatment of infectious processes.

2.1.3.2. Antifungal Effect

Fungal infections are very difficult to treat and long-term treatments with commercial antifungal products have side effects; therefore, it is necessary to avoid adverse effects. Zuccagnia-type propolis extracts were inhibitors of the growth of dermatophytes (Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrum) with MIC values between 16 and 125 μg/mL [40]. The main identified antifungal compounds were two chalcones, DHC and DHMC with MIC and minimal fungicidal concentration (MFC) values between 1.9 and 2.9 μg/mL.
The anti-Candida activity was also demonstrated (MIC of 125–500 μg/mL and MFC of 375–750 μg/mL) [44]. The anti-Candida activity of propolis was similar to that of dry extracts of Z. punctata [69] and could be attributed to DHC and DHMC [69,70]. Both chalcones could moderate fungal colonization and suppress the invasive mechanism of Candida, for example, by acting as an inhibitor of germ tube formation as well as biofilm formation and acting on exoenzyme activity [69]. According to the MIC obtained and considering the Tangarife-Castaño et al. classification, the extracts of the Zuccagnia-type propolis can be considered strong antifungals [71].

2.1.3.3. Nematicidal Activities

The Zuccagnia-type propolis showed an effect on adult Caenorhabditis elegans [44]. The LC50, defined as the concentration required for killing half of the C. elegans population within 24 h, was 70 μg/mL close to what the drug levamisole, a known anthelmintic drug (LC50 4.7 μg/mL), required.

2.1.3.4. Antioxidant Capacity

The reactive species of oxygen and nitrogen such as hydroxyl radical (HO•), hydrogen peroxide (H2O2), superoxide radical (O2•-), and nitric oxide (NO•) are a health hazard, since they can oxidize proteins, sugars, nucleic acids, and lipids. These free radicals contribute to several pathologies associated with oxidative stress such as inflammatory process, carcinogenesis, and metabolic syndrome.
Zuccagnia-type propolis extracts from Catamarca were active as HO• scavengers (Free radicals half scavenging concentration, SC50 values around 25 ± 5 µg/mL), showed high potential as an H2O2 scavenger and as O2•- scavengers with SC50 values of 115 and 205 µg/mL, and showed a remarkable capacity to scavenge nitrogen-reactive species such as NO• [58].
Zuccagnia-type propolis from Catamarca and Tucuman showed scavenging activity on ABTS•+ with SC50 values between 14 and 33 µg/mL [44,58], similar to the antioxidant capacity of Z. punctata extracts [72,73,74,75]. Other authors also evidenced the free-radical-scavenging activity of Monte region propolis extract on DPPH• (SC50 values between 10 and 43 µg/mL) [41,45,63,64].
The Zuccagnia-type propolis protected lipids from oxidation (inhibitory concentration of lipid oxidation in 50%, IC50 between 2 and 29 μg/mL) [44,64]. Avila et al. and Morán Vieyra et al. [76,77] reported the antioxidant properties and mechanisms of three structurally related flavonoids present in Zuccagnia-type propolis, 7-HF, DHC, and 3,7-DHF. The ABTS•+ and DPPH• scavenging reactivity trend was DHF > DHC > HF, which correlated with the electron donor capacity of the flavonoids. However, the O2 scavenging in aqueous buffered solution was significantly controlled by the fraction of neutral flavonoids through concerted proton-coupled electron transfer. The radical-scavenging reactivity trend was DHC > DHF > HF.

2.1.3.5. Effects on Pro-Inflammatory Mediators

The arachidonic acid (AA) pathway is involved in the inflammatory reactions. The best treatment for the inflammatory process would be to inhibit the pathway at several levels [78], for instance, phospholipase (PLA2), cyclooxygenases (COX2), and lipoxygenases (LOX), reducing the concentrations of prostanoids and leukotrienes. Specific inhibitors of the proinflammatory enzymes have side effects that prevent their consumption for long periods of time [79]. Several phenolic compounds were reported as inhibitors of COX2 and LOX [80,81]. In the present review, the findings of anti-inflammatory activity research of propolis from the Monte region have been summarized. The bioassays are focused on two most important topics: the effect on AA metabolizing enzymes and NOS, and the effect on the expression of proinflammatory enzymes.
The Zuccagnia-type propolis were effective as LOX activity inhibitors, with IC50 values of 70 ± 10 μg/mL), and COX inhibitors, with IC50 values around 100 ± 4 μg/mL [44]. These results are very promising; they inhibit LOX in percentages and concentrations similar to commercial anti-inflammatories, though at two levels of the AA pathway. The effect of a major component, 2′,4′ dihydroxy chalcone, present in propolis extract on COX2, was assayed. DHC was a potent inhibitor on COX2 and showed a dose-dependent response between 4 and 190 μM [82]. The inhibition of pro-inflammatory enzymes by DHC and its antioxidant capacity support the potential use of propolis as a medicine, which could be used to prevent the development of chronic inflammatory pathologies. Cell stimulation with bacterial lipopolysaccharide (LPS) induces pro-inflammatory cytokine production and iNOS protein expression in macrophages; consequently, it caused significant increase in NO production. Pretreatment of cells with Zuccagnia-type propolis extract inhibited NO overproduction in a dose-dependent manner (IC50 around 10 ± 0.5 μg/mL) and the iNOS expression with IC50 values around 30 μg/mL, while the COX2 expression was not affected [44]. This is the only report on anti-inflammatory activity for Argentine propolis; this activity was also reported to Brazilian, Chilean, Korean and Chinese propolis through other mechanisms [83].

2.1.3.6. Inhibitory Capacity of Enzymes Related to Metabolic Syndrome

Metabolic syndrome (MetS) is a clinical state that gathers several metabolic risk factors, including central obesity, i.e., excess of visceral adiposity, insulin resistance, hyperglycemia, hypertension, or dyslipidemia, characterized by high triglycerides level. Therapeutics for MetS are mainly based on lifestyle changes, often accompanied by pharmacological treatments. The modulation of inflammatory process and oxidative status in MetS is necessary to control this disorder, as well as the inhibition of enzymes involved in sugar and lipid metabolism [84]. Zuccagnia-type propolis extract showed strong antioxidant and anti-inflammatory activity (see Section 2.1.3.4 and Section 2.1.3.5), which can help reduce oxidative stress in MetS. It also showed inhibitory activity for α-glucosidase and lipase, followed by α-amylase [58]. Two chalcones, DHC and DHMC, chemical markers of Zuccagnia-type propolis and Z. punctata resin extract, were active, inhibiting lipase and α-glucosidase enzymes. Other authors reported that chalcones are potent α-glucosidase, α-amylase, and lipase inhibitors [85,86,87,88]. Oral administration of Z. punctata extract (plant source of Zuccagnia-type propolis) improves lipidic profile, reduces oxidative process and avoids vascular dysfunction in hypercholesterolemic rabbits [89,90].

2.1.4. Crop Protection and Post Harvesting Use

Antibacterial Activity

The Zuccagnia-type propolis were also active on phytopathogenic bacteria (Pseudomonas syringae, Pseudomonas corrugata, Xanthomonas campestris, and Erwinia carotovora), which are serious problems regarding crops or processed and fresh market tomatoes (Lycopersicon esculentum L.) [91] with MIC values between 5 and 40 μg gallic acid equivalent per milliliter GAE/mL. The main isolated antibacterial compound from this propolis was identified as DHC. Propolis-aqueous solutions, sprayed on tomato fruits, reduce the symptoms of disease. The effect of a propolis solution sprayed prior to or after inoculation with P. syringae pvar. tomato was similar, showing both curative and preventive effects. The bactericidal effect of propolis in vivo leads us to consider potential uses in agribusiness.

Antifungal Activity

The antifungal activity of the Zuccagnia-type propolis and the films containing ethanolic extract of this propolis against Penicillium digitatum, Penicillium expansum, Penicillium italicum, Alternaria alternata, Aspergillus carbonarius and Botrytis cinerea was assayed [92]. All the tested fungal pathogens were sensitive toward the propolis extract. The most striking effect could be seen on the growth of P. digitatum and B. cinerea, with MIC values of 0.14 and 0.17 mg/mL, respectively followed. A moderate effect was observed for Alternaria alternata, P. italicum, and A. carbonarius, with MIC values of around 0.40 mg/mL. The lowest sensitivity was exhibited by P. expansum (MIC values of 0.58 mg/mL). The sporulation of all fungal pathogens tested was affected by using 0.05 mg/mL of propolis extract [92].

2.1.5. Toxicity of Propolis from the Monte Region

Propolis did not show toxicity to model organisms such as Artemia salina and Allium cepa at concentrations where they showed biological activities. Genotoxicity was not found against the Salmonella typhimurium strains with and without metabolic activator, but they could inhibit the mutagenesis produced by two mutagens, isoquinoline and 4-nitro o-phenylenediamine. Similar results were reported for DHC, the main component of propolis from the Monte region in Argentina, evincing the potential of these bee products as anticancer agents [38]. Propolis extracts were not toxic against RAW 254.7 cell lines [44].
No toxicity studies were performed for Zuccagnia-type propolis extracts on experimentation animals; however, they were done for extracts of aerial parts from Zuccagnia punctata, from whose resins this propolis derives. The toxic effect on the liver and kidney of Z. punctata extract (1 mg/mice) was analyzed in mice. The activities of alanine transaminase and aspartate transaminase hepatic enzymes, as well as the levels of creatinine and urea in blood, were not changed with the administration of Z. punctata extract as compared with that of the control mice. Therefore, the report showed that the intake once or twice a day of 1 mg of plant extract for seven days did not result in toxicity [93]. Oral administration of Zuccagnia punctata extract at a dose of 2.5 mg/day to a hypercholesterolemic rabbit was not toxic as regards the kidney, and both the hepatic function and the hematological parameters did not change in relation with rabbit controls [89,90].

2.1.6. Fields of Application of Propolis from the Monte Region

Several reports indicate the great value of propolis to be used in the development of innovative products to improve health, functional food, food preservation, food packaging, and textile materials for biomedical application, to name but a few [6,94,95]. Several products containing propolis, such as medical devices, health foods, beverages, and cosmetics, among others, have been developed and commercialized. This is largely due to the numerous beneficial pharmacological properties of bee glue, i.e., its anti-inflammatory, anti-obesity, antitumor, antimicrobial, and antioxidant properties. A pharmaceutical product, ear drops containing Zuccagnia-type propolis extract as a bioactive, was developed for use in otitis [62]. The ear drops were standardized chemically, functionally, and microbiologically. The formulation showed inhibitory activity on pro-inflammatory enzymes, such as LOX (IC50 values 90 and 100 μg/mL), free-radical-scavenging effect (SC50 values 23 and 30 μg/mL), anthelmintic (LC50 values 70 and 71 μg/mL), anti-Candida (400 μg/mL), and antimicrobial activity against Gram-positive bacteria (200 μg/mL) during six month-storage. The content of chalcones, chemical markers of Zuccagnia-type propolis was quantified (DHC 3.54 mg/mL and DHMC 4.54 mg/mL) and its level was kept while stored at room temperature. The ear drops were not toxic. The results are noteworthy since the Zuccagnia-type propolis extract and ear drops developed could be an option for use in alternative medicine as an antibacterial, anti-Candida, anthelmintic, anti-inflammatory, and antioxidant.
In the last few years, propolis extracts were used in the conservation of food during storage. The propolis extracts can be added directly to foods or are administered superficially or in the form of edible films enriched in propolis extracts. These procedures reduce or eliminate pathogens or saprophytic microorganism from fish, fruit, vegetables, fruit juice, and milk. The propolis can contribute to keeping the quality of fruit, vegetables, meat, and fish during storage [24].
Edible, gelatin-based films containing Zuccagnia-type propolis were recently developed. They revealed remarkable antifungal activity against P. digitatum and B. cinerea and reduced the incidence of infection in raspberries stored at refrigerated temperatures for a long period of time [93].

3. Concluding Remarks and Future Trends

In this review, the wide potential of Argentine propolis is shown, especially the one whose botanical origin is Zuccagnia punctata, an endemic medicinal species of Argentina with unique characteristics. Additionally, substantial work has been done not only in determining its functional properties but also in its standardization from the chemical perspective. However, it is necessary to deepen the knowledge of chemical and functional properties, as well as the development of pharmaceutical, food, and cosmetic products derived from Argentine propolis.

4. Materials and Methods

This biographic research started in 1970 and ended at the end of November 2020; electronic databases such as http://www.scopus.com; http://www.scirus.com; http://scholar.google.com.ar; http://www.ncbi.nlm.nih.gov/pubmed; and http://www.sciencedirect.com were used. Searches were made by using key word combinations: propolis, Zuccagnia-type propolis, Argentine propolis, Zuccagnia punctata, Larrea, jarilla, biological activities, phytochemicals, toxicity, 2′,4′-dihydroxy chalcone, 2′,4′-dihydroxy-3′-methoxychalcone, antibacterial, antifungal, antioxidant, antibacterial, anti-inflammatory, chemo-preventive, antiulcer, and anti-biofilms, among others. The data were selected from systematic reviews and articles published in English. The bibliography was categorized according to its scope, namely botanical source, geographical origin, chemical composition, biological activity, or field of application. Data extraction was performed by all researchers, and the reported conclusions were achieved by consensus.

Author Contributions

Conceptualization, I.C.Z. and M.I.I.; methodology, A.L.S. and L.M.M.; resources, M.J.S. and M.I.I.; writing—original draft preparation, M.I.I. and I.C.Z.; writing—review and editing, I.C.Z., M.J.S., and M.I.I. All authors have read and agreed to the published version of the manuscript.

Funding

The authors would like to thank the following for their financial support: Secretaría de Ciencia, Arte e Innovación Tecnológica (SCAIT, PIUNT ), Argentina, Agencia Nacional de Promoción Científica y Técnica (ANPCyT) PICT 2017 Number 4436 and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PUE 2018- 0011), Argentina. M.J.S. acknowledges funds from FONDECYT 1180059.

Acknowledgments

Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV) and Facultad de Ciencias Naturales e IML are gratefully acknowledged.

Conflicts of Interest

The authors declare no conflict of interest.

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Metabolites 11 00076 g001 550
Figure 1. (A) Jarillal in the Monte region, (B) Zuccagnia punctata. Leaflet anatomy. Adaxial epidermis. Abaxial epidermis. Tg, capitate glandular trichome; e, epidermis.
Figure 1. (A) Jarillal in the Monte region, (B) Zuccagnia punctata. Leaflet anatomy. Adaxial epidermis. Abaxial epidermis. Tg, capitate glandular trichome; e, epidermis.
Metabolites 11 00076 g001
Metabolites 11 00076 g002 550
Figure 2. Functional properties of propolis from the Monte region.
Figure 2. Functional properties of propolis from the Monte region.
Metabolites 11 00076 g002
Table
Table 1. Chemical composition of Argentine propolis (province distribution).
Table 1. Chemical composition of Argentine propolis (province distribution).
Phenolic ComponentsSETCHSJCRNLPERSJMSFBA
Phenolic acid and derivates
Coumaric acidXXXXXXXNDX XXX
Caffeic acidNDNDNDNDNDNDXNDND XXX
Ferulic acidXXXXNDXXNDX XNDX
Cinnamic acidXXXNDNDXNDXX NDNDND
CAPEXXXNDNDXXNDX XNDND
Flavanones
PinobanksinXXXNDNDXXNDXXXNDND
PinocembrinXXXNDNDXXNDXXXNDX
Pinocembrin derivateNDNDNDNDNDNDNDNDNDNDNDNDX
NaringeninNDNDNDNDNDNDNDNDNDNDNDNDX
7-hydroxy-8-methoxyflavanoneNDNDNDNDNDNDNDNDNDXNDNDND
Flavone
ApigeninXXXXXXXXX XNDX
ChrysinXXXNDNDXXXXXXXX
TectochrysinXXXNDNDXXNDXXXNDND
Flavonol
GalanginXXXNDNDXXNDXXXXX
QuercetinXXXNDNDXNDNDX NDXX
KaempferolXXXNDNDXNDNDX NDNDND
KaempferideXXXNDNDXXNDX XNDND
Lignans
3′methyl-nordihydroguaiaretic acid (MNDGA)NDNDNDNDNDNDNDNDNDXNDNDND
nordihydroguaiaretic acid(NDGA)NDNDNDNDNDNDNDNDNDXNDNDND
3 (4-[4-(4-hydroxy-phenyl)-2,3-dimethyl-butyl]-benzene-1,2-diol)NDNDNDNDNDNDNDNDNDXNDNDND
meso-(rel 7S,8S,7′R,8′R)-3,4,3′,4′-tetrahydroxy7,7′-epoxylignanNDNDNDNDNDNDNDNDNDXNDNDND
5 (7S,8S,7′S,8′S)-3,3′,4′-trihy
droxy-4-methoxy-7,7′-epoxylignan		NDNDNDNDNDNDNDNDNDXNDNDND
SE: Santiago del Estero; T: Tucuman; C: Catamarca; CH: Chaco; J: Jujuy; S: Salta, RN: Rio Negro; SF: Santa Fe; LP: L Pampa; SJ: San Juan; M: Mendoza; ER: Entre Rios; BA: Buenos Aires; ND: non detected.
Table
Table 2. Compounds occurring in propolis from the Argentine Monte region.
Table 2. Compounds occurring in propolis from the Argentine Monte region.
CompoundsGeographical SitesReferences
Flavanones
7-hydroxyflavanone Tucumán, CatamarcaAgüero et al., 2010; Solorzano et al., 2012; Solórzano et al., 2017
7,8-dihydroxyflavanoneCatamarca. Tucumán Solórzano et al., 2017
3,7-dihydroxyflavanoneCatamarca. Tucumán Solórzano et al., 2017
4′,7-dihydroxyflavanone (liquiritigenin)TucumánSalas et al., 2016a
5,7-dihydroxyflavanone (pinocembrin)Catamarca, Tucumán Agüero et al., 2010, Solórzano et al., 2017
3,5,7-trihydroxyflavanone (pinobanksin)CatamarcaSolorzano et al., 2012
3,7,8-trihydroxydihydroflavanoneCatamarca, Tucumán Solórzano et al., 2017
5-hydroxy- 7- methoxyflavanone (pinostrobin)Tucumán, CatamarcaAgüero et al., 2010, Solorzano et al., 2012
7-hydroxy- 8-methoxyflavanoneCatamarca, Tucumán Vera et al., 2011; Agüero et al., 2010; Solorzano et al., 2012; Solórzano et al., 2017
7,4′-dihydroxy-5-methoxyflavanoneCatamarca, TucumánVera et al., 2011, Agüero et al., 2010; Solorzano et al., 2017
3β, 7-dihydroxy-5-methoxyflavanoneCatamarcaVera et al., 2011
7-hydroxy-5,8-dimethoxyflavanoneCatamarcaVera et al., 2011
pinobanksin-5-methyl ether (3,7-dihydroxy-5-methoxyflavanone)Catamarca, Tucumán Solórzano et al., 2017
Flavones
5,7-dihydroxyflavone (chrysin)Catamarca, Tucumán Solorzano et al., 2012; Solórzano et al., 2017
3,7-dihydroxyflavoneCatamarca, Tucumán Solórzano et al., 2017
3,5,7-trihydroxyflavone (galangin)Catamarca, Tucumán Agüero et al., 2010, Vera et al., 2011; Solórzano et al., 2017
3-hydroxy-7,8-dimethoxyflavoneTucumánAgüero et al., 2010, Vera et al., 2011
7-hydroxy-5,8 dimethoxyflavoneCatamarcaVera et al., 2011
5-hydroxy-4′,7-dimethoxyflavoneCatamarcaVera et al., 2011
3,7-dihydroxy-8-methoxyflavoneCatamarca, Tucumán Vera et al., 2011; Solorzano et al., 2012, Solórzano et al., 2017
3,5-dihydroxy-7-methoxyflavone (izalpinin)TucumánAgüero et al., 2010
3,5-dihydroxy-7,8-dimethoxyflavoneCatamarca, Tucumán Vera et al., 2011; Solorzano et al., 2012; Solórzano et al., 2017
4′, 5-dihydroxy-3,7,8-trimethoxyflavoneCatamarcaVera et al., 2011
3,4′,5-trihydroxy-7-methoxyflavone (rhamnocitrin)Catamarca, Tucumán Agüero et al., 2010; Solórzano et al., 2017
Chalcones
2′,4′-dihydroxychalcone (DHC)Catamarca, Tucumán Vera et al., 2011; Agüero et al., 2010; Solorzano et al., 2012, Salas et al., 2016a,b; Solórzano et al., 2017
2′,4′-dihydroxydihydrochalconeCatamarca, Tucumán Solórzano et al., 2017
4′-hydroxy-2′-methoxydihydrochalconeCatamarca, Tucumán Solórzano et al., 2017
2′,4′-dihydroxy-3′-methoxychalcone (DHMC)Catamarca, Tucumán Vera et al., 2011; Agüero et al., 2010; Solorzano et al., 2012; Salas et al., 2016a,b; Solórzano et al., 2017
2′,4′,4-trihydroxy-6′-methoxychalconeCatamarca, TucumánVera et al., 2011, Solórzano et al., 2017
Phenolic acids and esters
cinnamic acidTucumánSalas et al., 2016b
1,1-dimethylallyl caffeic acidCatamarca, Tucumán Solórzano et al., 2017
caffeoyl dihydrocaffeateTucumánSalas et al., 2016b
geranyl caffeateCatamarca, Tucumán Solórzano et al., 2019
pentenyl caffeateCatamarca, Tucumán Solórzano et al., 2019
benzyl caffeateCatamarca, Tucumán Solórzano et al., 2019
cinnamyl caffeateCatamarca, Tucumán Solórzano et al., 2019
methyl caffeateCatamarcaSolórzano et al., 2019
caffeic acid prenyl esterTucumánSalas et al., 2016b
3,4-dihydroxyphenethyl caffeic acid ester (teucrol)TucumánSalas et al., 2016b
1-methyl-3-(4-hydroxyphenyl)-propyl caffeic acid esterCatamarcaSolórzano et al., 2017
1-methyl-3-(4 -hydroxyphenyl)-propyl p-coumaric acid esterCatamarcaSolórzano et al., 2017
1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl caffeic acid esterCatamarcaSolórzano et al., 2017
4′-terbutyloxyphenyl p-coumaric acid esterCatamarca, Tucumán Solórzano et al., 2017
1-methyl-3-(4′-hydroxyphenyl)-propyl p-coumaric acid esterCatamarca, Tucumán Solórzano et al., 2017
3,7-dimethyl-2,6-octadienyl caffeic acid ester (geranyl caffeate)Catamarca, Tucumán Solórzano et al., 2017
1-methyl-3-(3′,4′-dihydroxyphenyl)-propyl ferulic acid esterCatamarca, Tucumán Solórzano et al., 2017
2-methyl-3-(3′-hydroxy-4′-methoxyphenyl)-propyl caffeic acid esterCatamarca, Tucumán Solórzano et al., 2017
Volatile compounds
trans-linalool oxide (furanoid)TucumánGonzalez et al., 2019
cis-linalool oxide (furanoid)TucumánGonzalez et al., 2019
(E)-anetholeTucumánGonzalez et al., 2019
LinaloolTucumánGonzalez et al., 2019
cis-linalyl oxide (pyranoid)TucumánGonzalez et al., 2019
p-cymen-8-olTucumánGonzalez et al., 2019
2,3,6-trimethylbenzaldehydeTucumánGonzalez et al., 2019
ChrysanthenoneTucumánGonzalez et al., 2019
p-mentha-1,5-dien-8-olTucumánGonzalez et al., 2019
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Zampini, I.C.; Salas, A.L.; Maldonado, L.M.; Simirgiotis, M.J.; Isla, M.I. Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient. Metabolites 2021, 11, 76. https://doi.org/10.3390/metabo11020076

AMA Style

Zampini IC, Salas AL, Maldonado LM, Simirgiotis MJ, Isla MI. Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient. Metabolites. 2021; 11(2):76. https://doi.org/10.3390/metabo11020076

Chicago/Turabian Style

Zampini, Iris Catiana, Ana Lia Salas, Luis M. Maldonado, Mario J. Simirgiotis, and María Inés Isla. 2021. "Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient" Metabolites 11, no. 2: 76. https://doi.org/10.3390/metabo11020076

APA Style

Zampini, I. C., Salas, A. L., Maldonado, L. M., Simirgiotis, M. J., & Isla, M. I. (2021). Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient. Metabolites, 11(2), 76. https://doi.org/10.3390/metabo11020076

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